Publications by authors named "Ning Ma"

1,046 Publications

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Intraplaque Enhancement Is Associated With Artery-to-Artery Embolism in Symptomatic Vertebrobasilar Atherosclerotic Diseases.

Front Neurol 2021 1;12:680827. Epub 2021 Sep 1.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

There are limited data regarding the characteristics of intracranial plaques according to stroke mechanism in the posterior circulation. This study aims to compare whether the plaque characteristics and baseline features are different in patients with artery-to-artery (A-to-A) embolism and those with parent artery disease in the intracranial vertebrobasilar atherosclerotic disease. From September 2014 to January 2017, patients with recent posterior circulation stroke due to intracranial vertebrobasilar atherosclerotic disease were retrospectively analyzed. Patients with the following eligibility criteria were included: (1) age ≥18 years old, (2) ischemic stroke in the vertebrobasilar territory, (3) 70-99% stenosis of the intracranial vertebral artery or basilar artery, and (4) two or more atherosclerotic risk factors. Patients with concomitant ipsilateral or bilateral extracranial vertebral artery >50% stenosis, cardio-embolism, or non-atherosclerotic stenosis were excluded. The plaque characteristics, including intraplaque compositions (intraplaque hemorrhage and intraplaque calcification), intraplaque enhancement, and remodeling index, were evaluated by using 3T high-resolution magnetic resonance imaging (HRMRI). The baseline features including vascular risk factors and the involved artery were collected. Patients were divided into A-to-A embolism and parent artery disease groups based on the diffusion-weighted images, T2-weighted images, or computed tomography. The plaque characteristics and baseline features were compared between the two groups. Among consecutive 298 patients, 51 patients were included. Twenty-nine patients had A-to-A embolism and 22 patients had parent artery disease. Compared with parent artery disease, the occurrence rates of intraplaque enhancement and intracranial vertebral involvement were higher in the A-to-A embolism group (79.3 vs. 36.4%; = 0.002 and 62.1 vs. 18.2%; = 0.002, respectively). Multivariable logistic regression analysis showed that intraplaque enhancement and intracranial vertebral artery plaques were also associated with A-to-A embolism (adjusted OR, 7.31; 95% CI 1.58-33.77; = 0.011 and adjusted OR, 9.42; 95% CI 1.91-46.50; = 0.006, respectively). Intraplaque enhancement and intracranial vertebral artery plaques seem to be more closely associated with A-to-A embolism than parent artery disease in patients with symptomatic intracranial vertebrobasilar disease. http://www.clinicaltrials.gov, identifier: NCT02705599.
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http://dx.doi.org/10.3389/fneur.2021.680827DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440987PMC
September 2021

NIR light-responsive bacteria with live bio-glue coatings for precise colonization in the gut.

Cell Rep 2021 Sep;36(11):109690

School of Life Sciences, Tianjin University, Tianjin 300072, China. Electronic address:

Recombinant bacterial colonization plays an indispensable role in disease prevention, alleviation, and treatment. Successful application mainly depends on whether bacteria can efficiently spatiotemporally colonize the host gut. However, a primary limitation of existing methods is the lack of precise spatiotemporal regulation, resulting in uncontrolled methods that are less effective. Herein, we design upconversion microgels (UCMs) to convert near-infrared light (NIR) into blue light to activate recombinant light-responsive bacteria (Lresb) in vivo, where autocrine "functional cellular glues" made of adhesive proteins assist Lresb inefficiently colonizing the gut. The programmable engineering platform is further developed for the controlled and effective colonization of Escherichia coli Nissle 1917 (EcN) in the gut. The colonizing bacteria effectively alleviate DSS-induced colitis in mice. We anticipate that this approach could facilitate the clinical application of engineered microbial therapeutics to accurately and effectively regulate host health.
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http://dx.doi.org/10.1016/j.celrep.2021.109690DOI Listing
September 2021

Mesenchymal stem cell conditioned medium attenuates oxidative stress injury in hepatocytes partly by regulating the miR-486-5p/PIM1 axis and the TGF-β/Smad pathway.

Bioengineered 2021 Dec;12(1):6434-6447

Department of Hepatobiliary-Pancreatic Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.

This study investigated the role of microRNA (miRNA) miR-486-5p in oxidative stress injury in hepatocytes under the treatment of mesenchymal stem cell conditioned medium (MSC-CM). The oxidative stress injury in hepatocytes (L02) was induced by HO. Human umbilical cord blood MSC-CM (UCB-MSC-CM) was prepared. The effects of UCB-MSC-CM on the proliferation, apoptosis, and inflammatory response in L02 cells were detected by Cell Counting Kit-8 (CCK-8) assay, flow cytometry analysis, and enzyme-linked immunosorbent assay (ELISA). Subsequently, the target of miR-486-5p was predicted using bioinformatics analysis, and the possible signaling pathway addressed by miR-486-5p was explored using western blot. We found that miR-486-5p expression was elevated following oxidative stress injury and was reduced after UCB-MSC-CM treatment. UCB-MSC-CM protected L02 cells against HO-induced injury by downregulation of miR-486-5p. Proviral integration site for Moloney murine leukemia virus 1 (PIM1) was verified to be targeted by miR-486-5p. UCB-MSC-CM upregulated the expression of PIM1 reduced by HO in L02 cells. Additionally, silencing PIM1 attenuated the protective effects of miR-486-5p downregulation against oxidative stress injury. We further demonstrated that UCB-MSC-CM inhibited the TGF-β/Smad signaling in HOtreated L02 cells by the miR-486-5p/PIM1 axis. Overall, UCB-MSC-CM attenuates oxidative stress injury in hepatocytes by downregulating miR-486-5p and upregulating PIM1, which may be related to the inhibition of TGF-β/Smad pathway.
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http://dx.doi.org/10.1080/21655979.2021.1972196DOI Listing
December 2021

Altered functional connectivity is related to impaired cognition in left unilateral asymptomatic carotid artery stenosis patients.

BMC Neurol 2021 Sep 13;21(1):350. Epub 2021 Sep 13.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 10070, China.

Background: Asymptomatic carotid artery stenosis (aCAS) impairs haemodynamic and cognitive functions; however, the relationship between these changes and brain network connectivity remains largely unknown. This study aimed to determine the relationship between functional connectivity and neurocognition in patients with aCAS.

Methods: We compared functional status in 14 patients with aCAS and 15 healthy controls using resting state functional magnetic resonance imaging sequences. The subjects underwent a full range of neuropsychological tests and a graphical theoretical analysis of their brain networks.

Results: Compared with controls, patients with aCAS showed significant decline in neuropsychological functions, particularly short-term memory (word-memory, p = .046 and picture-memory, p = .014). Brain network connectivity was lower in patients with aCAS than in the controls, and the decline of functional connectivity in aCAS patients was mainly concentrated in the left and right inferior frontal gyri, temporal lobe, left cingulate gyrus, and hippocampus. Decreased connectivity between various brain regions was significantly correlated with impaired short-term memory. Patients with aCAS showed cognitive impairment independent of known vascular risk factors for vascular cognitive impairment. The cognitive defects were mainly manifested in the short-term memory of words and pictures.

Conclusions: This study is the first of its kind to identify an association between disruption of functional connections in left carotid stenosis and impairment of short-term memory. The findings suggest that alterations in network connectivity may be an essential mechanism underlying cognitive decline in aCAS patients.

Clinical Trial Registration-url: Unique identifier: 04/06/2019, ChiCTR1900023610 .
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http://dx.doi.org/10.1186/s12883-021-02385-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436468PMC
September 2021

Echocardiographic characteristics of pediatric preexcitation cardiomyopathy.

Echocardiography 2021 Sep 9. Epub 2021 Sep 9.

Department of Ultrasound, Beijing Children's Hospital, Capital Medical University, National Center for children's Health, Beijing, China.

Preexcitation cardiomyopathy is a rare cardiac anomaly but it can be life threatening. It may occur in type B preexcitation syndrome. A patients with this condition typically has an enlarged left ventricle and reduced systolic function without tachycardia-associated cardiomyopathy. Echocardiography plays an important role in diagnosis in identifying this condition by revealing the "rebounce" movement of the inter-ventricular septum (IVS). We report echocardiographic findings of five pediatric patients with preexcitation cardiomyopathy. All of them had a radiofrequency catheter ablation (RFCA) and recovered during the follow-up.
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http://dx.doi.org/10.1111/echo.15181DOI Listing
September 2021

Beneficial Effect of Immune-Enhanced Enteral Nutrition on Immune Function in Patients With Severe Neurological Diseases: A Single-Center Randomized Controlled Trial.

Front Nutr 2021 23;8:685422. Epub 2021 Aug 23.

Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Undernutrition is the main reason for the use of artificial nutrition in patients with severe neurological diseases. However, the clinical and immunological outcomes of enteral nutrition supplemented with immunomodulatory nutrients in these patients remain unclear. In this single-center, randomized controlled study, 57 patients with severe neurological diseases were randomly divided into the following two groups according to the type of enteral nutrition they would receive: immune-enhancing (IE) ( = 27) and non-IE (NIE) ( = 30). The IE and NIE groups received enteral nutrition supplemented with immunomodulatory nutrients and standard enteral nutrition, respectively. We compared the nutritional status and the state of cellular immunity between the patients of the two groups. Clinical and immunological variables were evaluated following nutritional treatment. Feeding intolerance was lower in the IE than that in the NIE group ( = 0.04). However, there were no significant differences between the results of the two groups in terms of length of stay in the intensive care unit or hospital, extubation time, or body mass index ( > 0.05). The CD4+ T-lymphocyte count and CD4/CD8+ ratio in the peripheral blood increased significantly in the IE group. The expression of CD28 activated cell surface markers was higher in the IE than in the NIE group. In addition, increased plasma interferon-γ levels were recorded in the IE group, whereas the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-8, and IL-10 decreased. Immune-enhanced enteral nutrition could improve the immune status and feeding tolerance in patients with severe neurological diseases. www.chictr.org.cn/index.aspx, identifier: ChiCTR-IPR-17013909.
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http://dx.doi.org/10.3389/fnut.2021.685422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8419436PMC
August 2021

Humic acid mediated toxicity of faceted TiO nanocrystals to Daphnia magna.

J Hazard Mater 2021 08 15;416:126112. Epub 2021 May 15.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China. Electronic address:

Nano-bio interface is of great importance in dictating the interaction between the nanomaterials and biological system and thus the toxicity to aquatic organisms. Herein, two specific faceted TiO nanocrystals, {101} and {001} facet, were exposed to Daphnia magna to explore facet-dependent toxicological responses in aquatic environment. Due to the different influences on oxidative stress process, the half-maximal effective concentration (EC) value of {001} TiO (1.27 g L) to D. magna was less than that of {101} TiO (1.68 g L). Suwannee river humic acid (SRHA) could significantly reduce the oxidative stress responses of TiO nanocrystals and thus alleviate their toxicities to D. magna in aquatic environment. The protective effect of SRHA against TiO toxicity exhibited a facet-dependent manner. Compared to {101} TiO, a more obvious detoxification effect was observed for {001} TiO. The high SRHA concentration could endow both faceted TiO nanocrystals with a similar toxicity due to the formation of SRHA-corona on TiO surface. This facet-affected toxicity of nanomaterials in aquatic environment would provide us new insights in predicting the exposure risk of nanomaterials in nature waters.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126112DOI Listing
August 2021

Whole-blood magnesium and blood lipids are individually and jointly associated with an elevated likelihood of youngsters being overweight or obese: A matched case-control study using the propensity score.

Nutrition 2021 Jul 28;93:111425. Epub 2021 Jul 28.

Department of Endocrinology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address:

Objectives: Youngsters who are overweight or obese (YOO) have become an important global health concern. Some micronutrients may be modifiable influential factors. This study aimed to investigate the individual and joint association of whole-blood magnesium (WBMg) and total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), or high-density lipoprotein cholesterol (HDL-C) in YOO.

Methods: This is a propensity score matching-based case-control study. YOO was defined depending on age- and sex-specific body mass index z-score, calculated with SAS macros (%group_standard and %WHO2007) from the World Health Organization website. WBMg, blood lipids, and covariates were carefully measured by trained technicians using a whole-blood, five-element, basic analyzer and atomic absorption spectrometer or automatic biochemical analyzer. Locally weighted scattered plot smoothing and multivariable conditional logistic regression models were applied to estimate the associations of WBMg and blood lipids in YOO.

Results: WBMg was positively associated with YOO. The adjusted likelihood of YOO significantly increased by 21% (odds ratio: 1.21; 95% confidence interval [CI], 1.10-1.33) with per-interquartile range elevation of WBMg. Compared with the 1st quartile, adjusted odds ratios among youngsters in the 2, 3, and 4 quartiles of WBMg were 1.11 (95% CI, 0.92-1.35), 1.29 (95% CI, 1.06-1.57), and 1.47 (95% CI, 1.18-1.83), respectively. Furthermore, the relationship between WBMg and YOO was moderated by lipid profiles. Compared with those having lower (< median) WBMg and TC, TG, LDL-C, or higher (≥ median) HDL-C, youngsters with both higher WBMg and TC, TG, LDL-C, or lower HDL-C had higher YOO odds, which averagely increased by 188%, 250%, 339%, and 369%, respectively.

Conclusions: WBMg was an independent risk factor of YOO, and the associations were stronger among those with unhealthy blood lipids. Our findings can help to guide clinical and public health policies on the relevance of magnesium nutritional status.
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http://dx.doi.org/10.1016/j.nut.2021.111425DOI Listing
July 2021

Mechanistic process understanding of the self-assembling behaviour of asymmetric bolaamphiphilic short-peptides and their templating for silica and titania nanomaterials.

Nanoscale 2021 Aug 27;13(31):13318-13327. Epub 2021 Jul 27.

Centre for Bioengineering and Biotechnology, China University of Petroleum, Qingdao 266580, China.

Investigation of the self-assembly of peptides is critically important to clarify certain biophysical phenomena, fulfill some biological functions, and construct functional materials. However, it is still a challenge to precisely predict the self-assembled structures of peptides because of their complicated driving forces and various assembling pathways. In this work, to elucidate the effects of noncovalent interactions including hydrogen bonding, molecular geometry, and hydrophobic and electrostatic interactions on the peptide self-assembly, a series of asymmetric bolaamphiphilic short peptides consisting of Ac-EIK-NH (EIK), Ac-EIK-NH (EIK), Ac-KIE-NH (KIE) and Ac-KIE-NH (KIE) were designed and their self-assembling behaviors at different solution pH values were investigated systematically. The peptides self-assembled into twisted nanofibers under most conditions except for EIK in a strongly alkaline solution and KIE under a strongly acidic condition, in which they self-assembled into nanotubes via helical monolayer nanosheet intermediates. In particular, KIE nanotubes are formed under acidic conditions, and its diameters are ∼500 nm much greater than most of the self-assembled structures from bolaamphiphilic peptides. Moreover, reversible morphological transition between the nanotubes and twisted nanofibers was observed with the change in solution pH. Such tunable self-assembled structures and switchable surface properties of the asymmetric bolaamphiphilic short-peptides allow them to be used as templates to construct advanced materials. Silica and titania nanomaterials faithful to the peptide templates in morphology were prepared at ambient temperature. This work clearly elucidates the effects of noncovalent interactions on the peptide self-assembly and also provides new insights into the design and preparation of complicated inorganic materials from tunable organic templates.
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http://dx.doi.org/10.1039/d1nr01661jDOI Listing
August 2021

Association of residual stenosis after balloon angioplasty with vessel wall geometries in intracranial atherosclerosis.

J Neurointerv Surg 2021 Sep 1. Epub 2021 Sep 1.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China

Background: Percutaneous transluminal balloon angioplasty (PTBA) may be an alternative treatment for patients with symptomatic intracranial atherosclerotic disease (ICAD) refractory to medical treatment. This study aimed to investigate the association of vessel wall geometric characteristics on high-resolution magnetic resonance vessel wall imaging (MR-VWI) with low residual stenosis in patients with ICAD after PTBA.

Methods: Patients with symptomatic ICAD who underwent PTBA were prospectively and consecutively enrolled. High-resolution MR-VWI was performed before the PTBA. Vessel wall geometries of the target artery, including normalized wall index (NWI: wall area/vessel area × 100%), normalized wall thickness index (NWTI: mean wall thickness/vessel radius × 100%), and remodeling index (RI) were evaluated. Low residual stenosis was defined as postprocedural stenosis degree ≤50%. Perioperative complications including symptomatic ischemic stroke/intracranial hemorrhage, death, and arterial dissection were recorded. The baseline characteristics, vessel wall geometries, and perioperative complications were compared between the patients with low residual stenosis and high residual stenosis.

Results: Among 60 patients prospectively enrolled, low residual stenosis was achieved in 46 participants (77%). Three patients (5%) suffered from symptomatic ischemic stroke within 30 days. Multivariable logistic regression showed that a lower NWI and lower NWTI were associated with low residual stenosis after PTBA (adjusted OR 0.57, 95% CI 0.35 to 0.94, p=0.027; and adjusted OR 0.88, 95% CI 0.80 to 0.98, p=0.015).

Conclusions: Lower NWI and NWTI of the target artery on high-resolution MR-VWI were associated with low residual stenosis in patients with ICAD after PTBA.
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http://dx.doi.org/10.1136/neurintsurg-2021-017997DOI Listing
September 2021

Novel Diffusion-Weighted Imaging Score Showed Good Prognostic Value for Acute Basilar Artery Occlusion Following Endovascular Treatment: The Pons-Midbrain and Thalamus Score.

Stroke 2021 Aug 30:STROKEAHA120032314. Epub 2021 Aug 30.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China (L.L., Y.D., G.L., X.S., L.S., X.H., F.G., N.M., D.M., Z.M.).

Background And Purpose: Prognostic factors for outcome of endovascular treatment remains to be investigated in patients with acute basilar artery occlusion. We aimed to assess the prognostic value of a novel pretreatment diffusion-weighted imaging score: The Pons-Midbrain and Thalamus (PMT) score.

Methods: Eligible patients who underwent endovascular treatment due to acute basilar artery occlusion were reviewed. The PMT score was a diffusion-weighted imaging-based semiquantitative scale in which the infarctions of pons, midbrain, and thalamus were fully considered. The PMT score was assessed as well as the posterior circulation Acute Stroke Prognosis Early Computed Tomography Score and Brain Stem Score. Good outcomes were defined as a modified Rankin Scale score of ≤3 at 90-day and successful reperfusion as Thrombolysis in Cerebral Infarction grades 2b/3. The associations between baseline clinical parameters and good outcomes were evaluated with logistic regression.

Results: A total of 107 patients with pretreatment magnetic resonance imaging were included in this cohort. The baseline PMT score (median [interquartile range], 3 [1-5] versus 7 [5-9]; <0.001) and Brain Stem Score (median [interquartile range], 2 [1-4] versus 3 [2-5]; =0.001) were significantly lower in good outcome group; the posterior circulation Acute Stroke Prognosis Early Computed Tomography Score was higher in good outcome group without statistical significance. As a result of receiver operating characteristic curve analyses, the posterior circulation Acute Stroke Prognosis Early Computed Tomography Score showed poor prognostic accuracy for good outcome (area under the curve, 0.60 [95% CI, 0.49-0.71]; =0.081); The baseline PMT score showed significantly better prognostic accuracy for 90-day good outcome than the Brain Stem Score and National Institutes of Health Stroke Scale (area under the curve, 0.80 versus 0.68 versus 0.78, =0.003). In addition, favorable PMT score <7 (odds ratio, 22.0 [95% CI, 6.0-80.8], <0.001), Brain Stem Score <3 (odds ratio, 4.65 [95% CI, 2.05-10.55], <0.001) and baseline National Institutes of Health Stroke Scale <23 (odds ratio, 8.0 [95% CI, 2.5-25.6], <0.001) were associated with improved good outcome.

Conclusions: In patients with acute basilar artery occlusion following endovascular treatment, the pretreatment diffusion-weighted imaging based PMT score showed good prognostic value for clinical outcome.
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http://dx.doi.org/10.1161/STROKEAHA.120.032314DOI Listing
August 2021

Multi-proxy approaches to investigate cyanobacteria invasion from a eutrophic lake into the circumjacent groundwater.

Water Res 2021 Aug 19;204:117578. Epub 2021 Aug 19.

College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, PR China. Electronic address:

To verify whether cyanobacteria can travel from eutrophic lakes into the surrounding groundwater, a large-scale field investigation, laboratorial incubations, and quartz column penetration tests were carried out in Lake Taihu (China). High-throughput sequencing of 16S rRNA gene amplicons indicated that cyanobacteria operational taxonomic units (OTUs) were present at fifteen out of forty total wells in four cardinal directions at varying distances from the shore of Lake Taihu, up to a maximum of forty-three kilometers. Six cyanobacteria genera were detected including Microcystis, Dolichospermum, Phormidium, Leptolyngbya, Pseudanabaena and Synechococcus. The proportions of Phormidium, Microcystis and Synechococcus OTUs in the total cyanobacterial community were 45.2%, 32.2% and 19.4%, respectively. The qRT-PCR results showed that cyanobacterial abundance decreased with increasing distance from the shore of Lake Taihu. Based on the microscopic analysis of cultures inoculated with groundwater, we found Microcystis, Dolichospermum and Phormidium. Five cyanobacterial genera were able to penetrate columns filled with quartz particles ranging from 100∼200 μm. Finer layers of quartz sands were found to be impenetrable. The rating of infiltration capabilities was Microcystis > Synechococcus > Nostoc > Phormidium > Cylindrospermopsis. Deficient concentrations of microcystins were found (< 1 µg L) in the groundwater samples. Based on the consideration of different factors (cyanobacterial composition in Lake Taihu, peripheral groundwater, and algal soil crusts), it was deduced that Microcystis likely originated from the lake. Still, Phormidium was probably originated from the soil infiltration. These results suggest that cyanobacteria and their toxins could travel in the groundwater, but this is a size-dependent mechanism.
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http://dx.doi.org/10.1016/j.watres.2021.117578DOI Listing
August 2021

Physiological Functions of Threonine in Animals: Beyond Nutrition Metabolism.

Nutrients 2021 Jul 28;13(8). Epub 2021 Jul 28.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Threonine (Thr), an essential amino acid for animals and the limiting amino acid in swine and poultry diets, which plays a vital role in the modulation of nutritional metabolism, macromolecular biosynthesis, and gut homeostasis. Current evidence supports that the supplementation of Thr leads to benefits in terms of energy metabolism. Threonine is not only an important component of gastrointestinal mucin, but also acts as a nutritional modulator that influences the intestinal immune system via complex signaling networks, particularly mitogen-activated protein kinase (MAPK) and the target of the rapamycin (TOR) signal pathway. Threonine is also recognized as an indispensable nutrient for cell growth and proliferation. Hence, optimization of Thr requirement may exert a favorable impact on the factors linked to health and diseases in animals. This review focuses on the latest reports of Thr in metabolic pathways and nutritional regulation, as well as the relationship between Thr and relevant physiological functions.
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http://dx.doi.org/10.3390/nu13082592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8399342PMC
July 2021

Amino acids metabolism by rumen microorganisms: Nutrition and ecology strategies to reduce nitrogen emissions from the inside to the outside.

Sci Total Environ 2021 Aug 11;800:149596. Epub 2021 Aug 11.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, China. Electronic address:

For the ruminant animal industry, the emission of nitrogenous substances, such as nitrous oxide (NO) and ammonia (NH), not only challenges environmental sustainability but also restricts its development. The metabolism of proteins and amino acids by rumen microorganisms is a key factor affecting nitrogen (N) excretion in ruminant animals. Rumen microorganisms that affect N excretion mainly include three types: proteolytic and peptidolytic bacteria (PPB), ureolytic bacteria (UB), and hyper-ammonia-producing bacteria (HAB). Microbes residing in the rumen, however, are influenced by several complex factors, such as diet, which results in fluctuations in the rumen metabolism of proteins and amino acids and ultimately affects N emission. Combining feed nutrition strategies (including ingredient adjustment and feed additives) and ecological mitigation strategies of NO and NH in industrial practice can reduce the emission of nitrogenous pollutants from the ruminant breeding industry. In this review, the characteristics of the rumen microbial community related to N metabolism in ruminants were used as the metabolic basis. Furthermore, an effective strategy to increase N utilisation efficiency in combination with nutrition and ecology was reviewed to provide an inside-out approach to reduce N emissions from ruminants.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149596DOI Listing
August 2021

Apatinib inhibits cell proliferation and migration of osteosarcoma via activating LINC00261/miR-620/PTEN axis.

Cell Cycle 2021 Aug 23:1-14. Epub 2021 Aug 23.

Department of Orthopedics, The First Medical Center of General Hospital of PLA, Beijing, China.

Apatinib has been recently identified as a potential treatment option for osteosarcoma (OS). Nonetheless, the molecular mechanism of Apatinib in regulating OS progression remains unclear. To explore the downstream molecules that mediated the tumor-suppressive effect of Apatinib on OS. Expression levels of genes were detected by RT-qPCR and western blot assays. Functional assays including Transwell assay were applied to detect the proliferation, apoptosis and migration of OS cells. Molecular interactions were detected by luciferase reporter assay and RIP assay. Apatinib inhibited the proliferation and migration of OS cells. LINC00261 was down-regulated in OS cells but then up-regulated after the treatment by Apatinib. Silencing LINC00261 abrogated the suppressive effect of Apatinib on OS cell proliferation and migration. MicroRNA-620 (miR-620) could be sponged by LINC00261. Besides, miR-620 was up-regulated in OS cells and Apatinib treatment reduced miR-620 expression. Furthermore, LINC00261 acted as a competitive endogenous RNA (ceRNA) by sequestering miR-620 to up-regulate the expression of phosphatase and tensin homolog (PTEN). Moreover, Apatinib hindered cell proliferation and migration as well as the tumorigenesis of OS through LINC00261/miR-620/PTEN axis. Apatinib-enhanced LINC00261 restrained OS via miR-620/PTEN axis, indicating LINC00261 might promote the efficacy of Apatinib on OS.
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http://dx.doi.org/10.1080/15384101.2021.1949132DOI Listing
August 2021

4210 Da and 1866 Da polypeptides as potential biomarkers of liver disease progression in hepatitis B virus patients.

Sci Rep 2021 Aug 20;11(1):16982. Epub 2021 Aug 20.

Hebei Key Laboratory of Environment and Human Health, Department of Epidemiology and Statistics, School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China.

HBV infection is recognized as a serious global health problem, and hepatitis B virus infection is a complicated chronic disease leading to liver cirrhosis (LC) and hepatocellular carcinoma (HCC). New biochemical serum markers could be used to advance the diagnosis and prognosis of HBV-associated liver diseases during the progression of chronic hepatitis B into cirrhosis and HCC. We determined whether the 4210 Da and 1866 Da polypeptides are serum metabolite biomarkers of hepatopathy with hepatitis B virus. A total of 570 subjects were divided into five groups: healthy controls, those with natural clearance, and patients with CHB, LC, and HCC. The 1866 Da and 4210 Da polypeptides were measured by Clin-ToF II MALDI-TOF-MS. There were significant differences in 4210 Da and 1866 Da levels among the five groups (P < 0.001). For the differential diagnosis of CHB from normal liver, the areas under the receiver operating characteristic (ROC) curve of 4210 Da and 1866 Da and their combination via logistic regression were 0.961, 0.849 and 0.967. For the differential diagnosis of LC from CHB, the areas under the ROC curve were 0.695, 0.841 and 0.826. For the differential diagnosis of HCC from CHB, the areas under the ROC curve were 0.744, 0.710 and 0.761, respectively. For the differential diagnosis of HCC from LC, the areas under the ROC curve of 4210 Da and 1866 Da were 0.580 and 0.654. The positive rate of 1866 Da was 45.5% and 69.0% in AFP-negative HCC patients and that of 4210 Da was 60.6% 58.6% in AFP-negative HCC patients of the study HCC vs. CHB and HCC vs. LC. The 4210 Da and 1866 Da polypeptide levels were positively correlated with HBV DNA levels (P < 0.001, r = 0.269; P < 0.001, r = 0.285). The 4210 Da and 1866 Da polypeptides had good diagnostic value for the occurrence and progression of HBV-related chronic hepatitis, liver cirrhosis and hepatocellular carcinoma and could serve to accurately guide treatment management and predict clinical outcomes.
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http://dx.doi.org/10.1038/s41598-021-96581-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379215PMC
August 2021

Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients.

Medicine (Baltimore) 2021 Aug;100(33):e26879

Department of Endocrinology and Metabolism, First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, Jiangsu, China.

Abstract: The current study aimed to investigate circulating glucose-regulated protein 78 (GRP78) as well as CCAAT/enhancer-binding protein homologous protein (CHOP) concentrations in Chinese type 2 diabetes mellitus (T2DM) patients, especially those with microalbuminuria. We recruited 67 patients with T2DM and 63 control subjects. We determined circulating GRP78 and CHOP concentrations by ELISA, collected anthropometric data, and measured biochemical parameters in a clinical laboratory. Compared with control groups, patients with T2DM showed decreased circulating levels of GRP78 (0.21 [0.16-0.24] vs 0.16 [0.16-0.19] ng/mL, P < .01) and CHOP ([0.29 ± 0.02] vs [0.27 ± 0.03]ng/mL, P < .01). Reduction in circulating GRP78 and CHOP levels was more pronounced in patients with more severe categories of albuminuria. Amounts of circulating GRP78 correlated directly with serum fasting c-peptide, cystatin-c (Cys-c), creatinine (Cr), blood urea nitrogen (BUN), and uric acid, and inversely with glomerular filtration rates. Circulating CHOP level was positively correlated with age, Cr, BUN, Cys-c, and urinary microalbumin/creatinine (UmALB/Cr). Circulating GRP78 was predicted independently by Cr, BUN, serum uric acid, estimated glomerular filtration rate, and Cys-c, while CHOP depended on age, Cr, BUN, estimated glomerular filtration rate, UmALB/Cr, and Cys-c. After controlling for confounding factors, circulating GRP78 and CHOP expression were significantly associated with diabetic kidney disease (binary logistic regression, P < .01). Patients with T2DM showed increased circulating GRP78 and CHOP concentrations. Receiver operating characteristic areas under the curve for predicting diabetic kidney disease based on GRP78 and CHOP were 0.686 (95% CI: 0.558-0.813) and 0.670 (0.524-0.816), respectively.
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http://dx.doi.org/10.1097/MD.0000000000026879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376381PMC
August 2021

Prognostic significance of excision repair cross complementation group 1 rs2298881 in patients with gastric cancer receiving platinum-based chemotherapy: A PRISMA-compliant meta-analysis.

Medicine (Baltimore) 2021 Aug;100(33):e26850

Department of Oncological Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Gastric cancer (GC) is a strong cause of global cancer mortality. Nucleotide excision repair (NER) can modulate platinum-based chemotherapeutic efficacy by removing drug-produced DNA damage. Some studies have found a link between excision repair cross complementation group 1 (ERCC1) rs2298881, one gene in NER pathway, and response to chemotherapy. However, the results have been disputed.

Methods: We conducted a meta-analysis to reevaluate the association between polymorphisms of NER gene (ERCC1 rs2298881) and the clinical outcomes in gastric cancer patients receiving platinum-based chemotherapy. Searching PubMed, Web of Science, EMBASE, Google Scholar, and China National Knowledge Infrastructure, 2 independent searchers found all pertinent literatures up to May 1, 2021. We enrolled studies according to consistent selection criteria, extracted and vitrified data. Crude odds ratios (ORs) and hazard ratios (HRs) with 95% confidence interval (CI) were applied to evaluate the effect of ERCC1 rs2298881 on patients treated by platinum-based chemotherapy.

Results: By the data gathered from 6 independent studies, 1940 cases diagnosed with gastric cancer and treated with chemotherapy were included, containing 1208 Good-Responders and 732 Poor-Responders. With a comprehensive meta-analysis, we found that the patients with ERCC1 rs2298881A allele had a worse response to chemotherapy than those who with rs2298881C allele under allelic model (A vs C), with the pooled OR of 0.780 (95% CI: 0.611-0.996, P = .046). And our analysis indicated that AA genotype was associated with unfavorable overall survival (HR = 1.540, 95% CI = 1.106-2.144, P = .011) compared with CC genotype.

Conclusions: ERCC1 rs2298881 is suggested as a marker of clinical outcome in gastric cancer patients treated by platinum-based chemotherapy.
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http://dx.doi.org/10.1097/MD.0000000000026850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8376342PMC
August 2021

Magnetic resonance imaging for non-invasive clinical evaluation of normal and regenerated cartilage.

Regen Biomater 2021 Oct 17;8(5):rbab038. Epub 2021 Aug 17.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, No. 2005 Songhu Road, Yangpu District, Shanghai 200438, China.

With the development of tissue engineering and regenerative medicine, it is much desired to establish bioimaging techniques to monitor the real-time regeneration efficacy in a non-invasive way. Herein, we tried magnetic resonance imaging (MRI) to evaluate knee cartilage regeneration after implanting a biomaterial scaffold seeded with chondrocytes, namely, matrix-induced autologous chondrocyte implantation (MACI). After summary of the T2 mapping and the T1-related delayed gadolinium-enhanced MRI imaging of cartilage (dGEMRIC) and in the literature, these two MRI techniques were tried clinically. In this study, 18 patients were followed up for 1 year. It was found that there was a significant difference between the regeneration site and the neighboring normal site (control), and the difference gradually diminished with regeneration time up to 1 year according to both the quantitative T1 and T2 MRI methods. We further established the correlation between the quantitative evaluation of MRI and the clinical Lysholm scores for the first time. Hence, the MRI technique was confirmed to be a feasible semi-quantitative yet non-invasive way to evaluate the regeneration of knee articular cartilage.
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http://dx.doi.org/10.1093/rb/rbab038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369076PMC
October 2021

Influence of Epstein-Barr virus and human papillomavirus infection on macrophage migration inhibitory factor and macrophage polarization in nasopharyngeal carcinoma.

BMC Cancer 2021 Aug 18;21(1):929. Epub 2021 Aug 18.

Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Tsu, 514-8507, Japan.

Background: To assess the effects of Epstein-Barr virus (EBV) and human papillomavirus (HPV) infection on the tumor microenvironment, we examined the relationship between viral infection status, macrophage migration inhibitory factor (MIF), and tumor-associated macrophages in nasopharyngeal carcinoma (NPC).

Methods: A tissue microarray containing 150 cores from 90 patients with NPC and six with chronic inflammation was used. EBV and HPV status were detected using in situ hybridization with commercial EBER1 and HPV16/18 probes. Immunofluorescence double staining of MIF, pan-macrophage marker CD68, M1 macrophage marker CD11c, and M2 macrophage marker CD163 were analyzed using the same tissue microarray. The levels of these markers between NPC and inflammation cases and between tumor nests and stroma were compared. Correlations among these markers were analyzed.

Results: We found EBER1(+) cases in 90% of NPC patients, including 10% EBV/HPV co-infection. M1 macrophages mainly infiltrated the tumor nest, while M2 macrophages infiltrated the tumor stroma. We found a significant positive correlation between EBER1 levels and MIF levels in tumor nests and a significant positive correlation between HPV16/18 and CD11c(+) cell levels in NPC tissues.

Conclusions: It is suggested that MIF is associated with EBV, and M1 macrophage infiltration is affected by HPV status in NPC.
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http://dx.doi.org/10.1186/s12885-021-08675-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371777PMC
August 2021

Microflora and umami alterations of different packaging material preserved mushroom (Flammulina filiformis) during cold storage.

Food Res Int 2021 09 1;147:110481. Epub 2021 Jun 1.

College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address:

In order to clarify the effect of nanocomposite-based packaging (NP) on umami and microflora characteristics of F. filiformis during cold storage, the contents of umami amino acids and 5'-nucleotides, equivalent umami concentration (EUC), and microflora succession were investigated. Results showed that NP could delay the degradation of umami components and inhibit bacterial growth in F. filiformis. At the initial stage, the dominant bacteria were Lactobacillus, Thermus and Acinetobacter. After 15 days of storage, the bacteria count in NP reached 7.63 lg cfu/g, which was significantly (P < 0.05) lower than that in control, and the major bacterial communities of packaged F. filiformis were Ewingella, Serratia and Pseudomonas. Moreover, the correlation analysis showed that Lactobacillus, Brevibacillus and Okibacterium were negatively correlated with AMP and IMP 5-nucleotides. Present work suggested that NP could enhance the umami flavor formation and improve the microbial community structure of F. filiformis, resulting in a better commercial quality. The results provided theoretical basis for large-scale applications of NP.
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http://dx.doi.org/10.1016/j.foodres.2021.110481DOI Listing
September 2021

Acupuncture Treatment for Social Defeat Stress.

Front Behav Neurosci 2021 28;15:685433. Epub 2021 Jul 28.

Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka, Japan.

Depression is a mood disorder characterized by disordered affect, thoughts, cognition, and behavior. Antidepressant therapy is often the primary treatment for depression. However, antidepressant therapy may cause unwanted side effects, and its effects are slow. Therefore, some patients are seeking alternative treatments for depression, such as acupuncture. However, there are many unclear points regarding the mechanism of the effect of acupuncture on depression. In recent years, we have reported that acupuncture improves the symptoms of mild depression induced by water-immersion stress in a rat model and depression induced by forced swimming in a mouse model. In this study, we examined the effect of acupuncture on the symptoms of social defeat stress (SDS)-induced depression in mice that most closely resemble human symptoms. In this study, we investigated the preventive and therapeutic effects of acupuncture as part of GV20 "Bai-Hui" and Ex-HN3 "Yintang" on model mice with depression induced by SDS. To examine the mechanism of the preventive and therapeutic effects of acupuncture on depression model mice, we examined the expression of neurotrophic factors in the brains of SDS mice. Two weeks of simultaneous acupuncture stimulation as part of GV20 and Ex-HN3 restored SDS-reduced brain-derived neurotrophic factor (BDNF), neurotrophin (NT)-3, and NT-4/5 expression, which was not observed with antidepressants. In contrast, acupuncture stimulation suppressed nerve growth factor (NGF) expression induced by SDS. These results suggest that acupuncture treatment could be effective in correcting the imbalance in the expression of neurotrophic factors. Furthermore, the effects of acupuncture on the expression of neurotrophic factors appear earlier than those of antidepressants, suggesting that it may be a useful treatment for depression.
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http://dx.doi.org/10.3389/fnbeh.2021.685433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355549PMC
July 2021

Integrating basic and applied researches of SARS-CoV-2 into the medical molecular biology teaching.

Biochem Mol Biol Educ 2021 Aug 12. Epub 2021 Aug 12.

The Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.

Molecular biology is an important course for medical postgraduates to understand the practice of modern molecular medicine. At present, molecular biology has been widely used in the prevention, diagnosis, and treatment of clinical medicine. The COVID-19 pandemic forced all universities suspending academic activities and online teaching all over the world, which is a kind of crisis and has become people's memory of the times. The efforts from all sectors of society, include government, research institutions, and enterprises, have gave medical students a profound impact. The integration of memory of the times into molecular biology teaching will enhance students' learning interest and understanding effect.
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http://dx.doi.org/10.1002/bmb.21573DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8426797PMC
August 2021

Clinical characteristics, outcome and prognostic factors in critically ill patients with lupus nephritis.

Clin Exp Rheumatol 2021 Jul 24. Epub 2021 Jul 24.

Department of Infectious Disease, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Objectives: To describe the clinical characteristics and outcome of patients with lupus nephritis (LN) in intensive care unit (ICU), identify prognostic factors and construct a predictive model of in-ICU survival.

Methods: A total of 505 ICU admissions of lupus patients were screened and LN patients confirmed by renal biopsy were enrolled. Clinical characteristics and outcome of patients in ICU were collected. A logistic regression analysis was performed to identify independent prognostic factors and a nomogram was plotted to construct a predictive model.

Results: A total of 70 patients with LN were enrolled. The median age of the patients was 28.5 years, and the median course of LN was two months. Renal pathology classes indicated that 38 patients were class IV, 11 were class IV+V, and 10 were class III. The most common primary cause of ICU admission was infection in 40 patients, followed by LN in 11 patients. Forty-one patients died in ICU. The multivariate analyses revealed that lactic acid (OR 1.682 [2.130-17.944], p=0.001), gamma-glutamyl transpeptidase (OR 1.057 [1.009-1.107], p=0.020), APACHE II (OR 3.852 [1.176-12.618], p=0.026), vasopressor (OR 10.571 [1.615-69.199], p=0.014) and platelet count (OR 0.967 [0.941-0.993], p=0.013) were independently associated with ICU survival of critical LN patients. A predictive model was constructed and validated.

Conclusions: This study is the first to elucidate the features and identify prognostic factors in critically ill patients with LN. These findings could help clinicians to early identify high-risk patients of mortality, which consequently may reduce the mortality of critically ill patients with LN.
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July 2021

Development of a Novel Simple Model to Predict Mortality in Patients With Systemic Lupus Erythematosus Admitted to the Intensive Care Unit.

Front Med (Lausanne) 2021 22;8:689871. Epub 2021 Jul 22.

Department of Infectious Disease, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Patients with systemic lupus erythematosus (SLE) may sometimes require admission to the intensive care unit (ICU), and the outcome is poor. The aim of this study was to explore the clinical features of patients with SLE in the ICU, identify prognostic factors, and develop and evaluate a prognostic model to predict in-ICU mortality of patients with SLE. This was a single center retrospective study in a tertiary medical institution in China. A total of 480 SLE patients with 505 ICU admissions from 2010 to 2019 were screened, and 391 patients were enrolled. The clinical feature and outcomes of the patients were analyzed. According to the random number table, patients were divided into two mutually exclusively groups named derivation ( = 293) and validation ( = 98). Prognostic factors were identified by a Cox model with Markov Chain Monte Carlo simulation and evaluated by latent analysis. The risk score was developed based on the derivation group and evaluated using the validation group. Among the 391 patients, 348 (89.0%) patients were females. The median age of patients was 34 years, and the median course of SLE was 6 months. The median APACHE II and SLEDAI were 17 and 10, respectively. The average in-ICU mortality was 53.4% (95% CI, 48.5-58.4%). A total of 186 patients were admitted to the ICU due to infection. Pneumonia (320/391, 81.8%) was the most common clinical manifestation, followed by renal disease (246/391, 62.9%). Nine prognostic factors were identified. The model had C statistic of 0.912 (95% CI, 0.889-0.948) and 0.807 (95% CI 0.703-0.889), with predictive range of 5.2-98.3% and 6.3-94.7% for the derivation and validation groups, respectively. Based on distribution of the risk score, 25.3, 49.5, and 25.2% of patients were stratified into the high, average, and low-risk groups, with corresponding in-ICU mortality of 0.937, 0.593, and 0.118, respectively. Nine prognostic factors including age, white blood cell count, alanine transaminase, uric acid, intracranial infection, shock, intracranial hemorrhage, respiratory failure, and cyclosporin A/tacrolimus usage were identified. A prognostic model was developed and evaluated to predict in-ICU mortality of patients with SLE. These findings may help clinicians to prognostically stratify patients into different risk groups of in-ICU mortality, and provide patients with intensive and targeted management.
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http://dx.doi.org/10.3389/fmed.2021.689871DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339434PMC
July 2021

The bile acid-activated retinoic acid response in dendritic cells is involved in food allergen sensitization.

Allergy 2021 Aug 8. Epub 2021 Aug 8.

Inflammation & Allergic Diseases Research Unit, Affiliated Hospital of Southwest Medical University, Sichuan, China.

Background: Alteration of commensal microbiota is highly correlated with the prevalence of allergic reactions to food in the gastrointestinal tract. The mechanisms by which microbiota modulate food allergen sensitization in the mucosal site are not fully understood.

Methods: We generate DCs specific knockout of retinoic acid receptor α (Rara) gene mice (DC KO Rara) to evaluate food sensitization. The bile acid-activated retinoic acid response was evaluated by flow cytometry, real-time RT-PCR and Illumina transcriptome sequencing. The global effect of Abx treatment on BA profiles in the mucosal lymph tissue mLN in mice was examined by UPLC-MS analysis.

Results: In this study, we demonstrate that depletion of commensal gut bacteria leads to enhanced retinoic acid (RA) signaling in mucosal dendritic cells (DCs). RA signaling in DCs is required for the production of food allergen-specific IgE and IgG1. Antibiotics induced an enlarged bile acid (BA) pool, and dysregulated BA profiles contributed to enhanced RA signaling in mucosal DCs. BA-activated RA signaling promoted DC upregulation of interferon I signature, RA signature, OX40L, and PDL2, which may lead to T helper 2 differentiation of CD4+ T cells. BA-activated RA signaling involved the farnesoid X receptor and RA receptor α (RARa) interaction. Depletion of bile acid reduces food allergen specific IgE and IgG1 levels in mice.

Conclusion: Our research unveils a mechanism of food sensitization modulated by BA-RA signaling in DCs, which suggests a potential new approach for the intervention of food allergic reactions.
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http://dx.doi.org/10.1111/all.15039DOI Listing
August 2021

Logic Analysis of How the Emergency Management Legal System Used to Deal with Public Emerging Infectious Diseases under Balancing of Competing Interests-The Case of COVID-19.

Healthcare (Basel) 2021 Jul 6;9(7). Epub 2021 Jul 6.

Beijing Key Laboratory of Environment and Viral Oncology, College of Life Science and Chemistry, Faculty of Environment and Life, Beijing University of Technology, Beijing 100124, China.

Development of measures for mitigating public emerging infectious diseases is now a focal point for emergency management legal systems. COVID-19 prevention and containment policies can be considered under the core goal of social and individual interests. In this study we analyzed the complexity between individual and public interests as they conflict when implementing disease preventative measures on an epidemic scale. The analysis was used to explore this complex landscape of conflicting social, public, and legal interests to quantify the potential benefits of public acceptance. Here we use the large-scale COVID-19 epidemic backdrop to examine legal norms of the emergency management legal framework. We find that the implementation of emergency management legal system measures involves the resolution of both direct and indirect conflicts of interest among public groups, individual groups, and various subsets of each. When competing interests are not balanced, optimal policies cannot be achieved to serve and safeguard shared social and community stability, whereas effective social outcomes are obtainable through the development of targeted policies as defined within the emergency management legal system. A balanced legal framework in regards to emergency management legal norms can more effectively serve to mitigate and prevent the continued spread of emerging infectious diseases. Further developing innovative procedural mechanisms as a means to ensure emergency response intervention should take into account the weighted interest of the different social parties to determine priorities and aims to protect legitimate public interests.
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http://dx.doi.org/10.3390/healthcare9070857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306266PMC
July 2021

Safety and Efficacy of Direct Angioplasty in Acute Basilar Artery Occlusion Due to Atherosclerosis.

Front Neurol 2021 19;12:651653. Epub 2021 Jul 19.

Department of Interventional Neuroradiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Endovascular treatment (EVT) is one of the promising treatment options in patients with intracranial atherosclerotic disease (ICAD)-related basilar artery occlusion (BAO). In this study, we compared the safety and efficacy of direct angioplasty (DA) with stent-retriever thrombectomy (SRT) with or without rescue treatment in ICAD-related BAO. We retrospectively evaluated 187 patients who underwent EVT for BAO from January 2012 to July 2018. We identified patients who underwent EVT due to ICAD-related BAO. Patients who accepted SRT with or without rescue treatment were classified into the SRT group. Patients treated with DA with or without stent placement were classified into DA group. Clinical and laboratory findings and outcomes were compared between groups. A total of 108 patients were enrolled, among them 77 underwent SRT and 31 underwent DA; 61 (79.2%) SRT group patients underwent angioplasty with or without stent placement. Compared with patients in the SRT group, those in the DA group experienced a significantly shorter procedure time [60 min (60-120 min) vs. 120 min (60-120 min); = 0.038] and a lower number of device passes [2 passes (1-2 passes) vs. 3 passes (2-4 passes); < 0.001]. No significant differences in balloon angioplasty (35.5 vs. 22.1%; = 0.150), emergent stent placement (64.5 vs. 57.1%; = 0.481), successful recanalization (93.5 vs. 85.7%; = 0.340), embolization in distal or new territory (3.2 vs. 9.1%, = 0.314), and reocclusion (22.6 vs. 9.1%; = 0.109) among DA and SRT groups were found. Additionally, no differences in symptomatic intracranial hemorrhage incidence [adjusted odds ratio (OR), 0.74; 95% CI, 0.06-9.44; = 0.815], functional independence (adjusted OR, 1.44; 95% CI, 0.50-4.16; = 0.497), and mortality rate (adjusted OR, 0.36; 95% CI, 0.06-2.04; = 0.247) were noted among groups. In certain patients with ICAD-related BAO, DA may shorten procedure time and reduce required device passes compared to SRT. In this study, DA was retrospectively found to be of similar safety and efficacy as SRT.
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http://dx.doi.org/10.3389/fneur.2021.651653DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326335PMC
July 2021

Continuing Cetuximab Bevacizumab plus chemotherapy after first progression in wild-type , and metastatic colorectal cancer: a randomized phase II trial.

J Cancer 2021 26;12(17):5268-5274. Epub 2021 Jun 26.

Department of Oncology, Affiliated cancer hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, China 450008.

To evaluate the clinical efficacy of continuing cetuximab vs bevacizumab plus chemotherapy crossover after first progression to cetuximab regimen in wild-type , and mCRC, we conducted this prospective, open-label and randomized phase 2 trial in three cancer centers from Oct 1, 2016 to July 1, 2020. Eligibility criteria included documented progressive disease during or after first-line treatment with cetuximab regimen; second biopsy confirmed as , and wild-type mCRC. Patients were randomized to arm A (cetuximab+chemo) or arm B (bevacizumab+chemo) with second-line chemotherapy crossover. The primary end point was progression free survival (PFS). Secondary end points included objective response rate (ORR), overall survival (OS) and toxicity. Tissue VEGFA, ERBB2 and MET mRNA were examined by real time RT-PCR. A total of 104 patients (53 in arm A and 51 in arm B) were enrolled. Median PFS was 7.7 months (95% CI: 6.5-8.9) for arm A and 6.3 months (95% CI: 4.5-8.1) for arm B (p=0.931). Median OS was 18.2 months (95% CI: 14.5-21.9) for arm A and 16.4 months (95% CI: 14.2-18.6) for arm B (p=0.339). The ORR was 28.3% and 19.6% in arm A and arm B (p=0.31), respectively. MET mRNA was highly expressed in the cetuximab-progressed tumors, but treatment responsiveness to cetuximab or bevacizumab in each arm was not correlated with the MET expression level. The results showed no significant difference in PFS, OS and ORR between the two arms, but a trend in favor of the cetuximab continuation plus chemotherapy crossover was examined in all end points. High expression of MET in cetuximab-progressed tumors may indicate an existence of MET-dependent tumor cell population.
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http://dx.doi.org/10.7150/jca.60014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8317523PMC
June 2021

A Digital Case-Finding Algorithm for Diagnosed but Untreated Hep C: A Tool for Increasing Linkage to Treatment and Cure.

Hepatology 2021 Aug 1. Epub 2021 Aug 1.

Division of Liver Medicine, Icahn School of Medicine Mount Sinai, New York, NY, USA.

Background And Aims: Although chronic hepatitis C virus (HCV) infection increases mortality, thousands of patients remain diagnosed-but-untreated (DBU). We aimed to a) develop a DBU phenotyping algorithm, b) use it to facilitate case finding and linkage to care, and c) identify barriers to successful treatment.

Approach And Results: We developed a phenotyping algorithm using JAVA and SQL and applied it to ~2.5 million EPIC electronic medical records (EMRs) (data entered 1/2003-12/2017). Approximately 72,000 EMRs contained an HCV international classification of diseases code and/or diagnostic test. The algorithm classified 10,614 cases as DBU (HCV RNA-positive and alive). Its positive and negative predictive values were 88% and 97%, respectively, as determined by manual-review of 500 EMRs randomly selected from the ~72,000. Navigators reviewed the charts of 6,187 algorithm-defined DBUs and they attempted to contact potential treatment candidates by phone. By 6/2020, 30% (n=1,862) had completed an HCV-related appointment. Outcomes analysis revealed that DBU patients enrolled in our care coordination program were more likely to complete treatment-72% (n=219) vs. 54% (n=256), p<0.001-and to have a verified sustained virological response-67% vs. 46%, p<0.001-than other patients. Forty-eight percent (n=2,992) of DBU patients could not be reached by phone, which was a major barrier to engagement. Nearly half of these patients had fibrosis-4 scores ≥2.67, indicating significant fibrosis. Multivariable logistic regression showed that DBUs who could not be contacted were less likely to have private insurance than those who could: 18% vs. 50%, p<0.001.

Conclusions: The digital DBU case-finding algorithm efficiently identified potential HCV treatment candidates, freeing resources for navigation and coordination. The algorithm is portable and accelerated HCV elimination when incorporated in our comprehensive program.
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http://dx.doi.org/10.1002/hep.32086DOI Listing
August 2021
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