Publications by authors named "Ning Li"

4,988 Publications

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Effects of homoeologous exchange on gene expression and alternative splicing in a newly formed allotetraploid wheat.

Plant J 2022 Jun 28. Epub 2022 Jun 28.

Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024, China.

Homoeologous exchange (HE) is a major mechanism generating post-polyploidization genetic variation with important evolutionary consequences. However, the direct impacts of HE on gene expression and transcript diversity in allopolyploids without the intertwined evolutionary processes remains to be fully understood. Here, we analyzed high-throughput RNA-seq data of young leaves from plant groups of a synthetic allotetraploid wheat (AADD), which contained variable numbers of HEs. We aimed to investigate if and to which extent HE directly impacts gene expression and alternative splicing (AS). We found that HE impacts expression of genes located within HE regions primarily via cis-acting dosage effect, which led to significant changes in the total expression level of homoeologous gene pairs, especially for homoeologs whose original expression was biased. In parallel, HE also influences expression of a large number of genes residing in non-HE regions by trans-regulation leading to convergent expression of homoeologs. Intriguingly, when taking into account of the original relative homoeolog expression states, homoeolog pairs under trans-effect are more prone to manifesting convergent response to the HEs whereas those under cis-regulation trended to show further exacerbated subgenome-biased expression. Moreover, HE-induced quantitative, largely individual-specific, changes of alternative splicing (AS) events were detected. Similar to homoeologous expression, homoeo-AS events under trans effect were more responsive to HE. HE therefore exerts multifaceted immediate effects on gene expression and, to a less extent, on individualized transcript diversity in nascent allopolyploidy.
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http://dx.doi.org/10.1111/tpj.15886DOI Listing
June 2022

Remote Manipulation of ROS-Sensitive Calcium Channel Using Near-Infrared-Responsive Conjugated Oligomer Nanoparticles for Enhanced Tumor Therapy .

Nano Lett 2022 Jun 27. Epub 2022 Jun 27.

School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300401, PR China.

The regulation of reactive oxygen species (ROS)-sensitive calcium (Ca) channels is of great significance in the treatment of tumors. Here, a simple ROS generation system is developed to activate ROS-sensitive ion channels for enhancing calcium-cascade-mediated tumor cell death under near-infrared (NIR) light irradiation. Upon irradiation with an 808 nm laser, a low-lethality amount of ROS facilitates plasmid transient potential receptor melastatin-2 (pTRPM2) gene release via cleavage of the Se-Se bonds, which contributed to enhancing the expression of TRPM2 in tumor cells. Meanwhile, ROS could potently activate TRPM2 for Ca influx to inhibit early autophagy and to further induce intracellular ROS production, which ultimately led to cell death in TRPM2 expressing tumor cells. Both and data show that nanoparticles have an excellent therapeutic effect on cancer upon NIR light. This work presents a simple modality based on NIR light to remotely control the ROS-sensitive ion channel for cancer therapy.
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http://dx.doi.org/10.1021/acs.nanolett.2c01472DOI Listing
June 2022

Optimal control and cost-effectiveness analysis for a COVID-19 model with individual protection awareness.

Authors:
Yiran Yuan Ning Li

Physica A 2022 Jun 22:127804. Epub 2022 Jun 22.

College of Science, Northeastern University, Shenyang 110819, Liaoning, China.

This paper is focused on the design of optimal control strategies for COVID-19 and the model containing susceptible individuals with awareness of protection and susceptible individuals without awareness of protection is established. The goal of this paper is to minimize the number of infected people and susceptible individuals without protection awareness, and to increase the willingness of susceptible individuals to take protection measures. We conduct a qualitative analysis of this mathematical model. Based on the sensitivity analysis, the optimal control method is proposed, namely personal protective measures, vaccination and awareness raising programs. It is found that combining the three methods can minimize the number of infected people. Moreover, the introduction of awareness raising program in society will greatly reduce the existence of susceptible individuals without protection awareness. To evaluate the most cost-effective strategy we performed a cost-effectiveness analysis using the ICER method.
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http://dx.doi.org/10.1016/j.physa.2022.127804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9216683PMC
June 2022

Interfacially Locked Metal Aerogel Inside Porous Polymer Composite for Sensitive and Durable Flexible Piezoresistive Sensors.

Adv Sci (Weinh) 2022 Jun 24:e2201912. Epub 2022 Jun 24.

The Key Laboratory of Low-Carbon Chemistry & Energy Conservation of Guangdong Province, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, State Key Laboratory of Optoelectronic Materials and Technologies, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou, 510006, P. R. China.

Flexible pressure sensors play significant roles in wearable devices, electronic skins, and human-machine interface (HMI). However, it remains challenging to develop flexible piezoresistive sensors with outstanding comprehensive performances, especially with excellent long-term durability. Herein, a facile "interfacial locking strategy" has been developed to fabricate metal aerogel-based pressure sensors with excellent sensitivity and prominent stability. The strategy broke the bottleneck of the intrinsically poor mechanical properties of metal aerogels by grafting them on highly elastic melamine sponge with the help of a thin polydimethylsiloxane (PDMS) layer as the interface-reinforcing media. The hierarchically porous conductive structure of the ensemble offered the as-prepared flexible piezoresistive sensor with a sensitivity as high as 12 kPa , a response time as fast as 85 ms, and a prominent durability over 23 000 compression cycles. The excellent comprehensive performance enables the successful application of the flexible piezoresistive sensor as two-dimensional (2D) array device as well as three-dimensional (3D) force-detecting device for real-time monitoring of HMI activities.
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http://dx.doi.org/10.1002/advs.202201912DOI Listing
June 2022

Approaches to Safety Evaluation of Baby Wipes.

Glob Pediatr Health 2022 17;9:2333794X221105261. Epub 2022 Jun 17.

Winton Hill Business Center, Cincinnati, OH, USA.

Disposable baby wipes manufactured by Procter & Gamble, soft sheets bearing lotion that is balanced to maintain natural skin pH, are convenient for cleaning the diaper area and a quick cleanup on baby's face and hands.

Objective: Develop a rigorous safety assessment process to ensure that baby wipes are safe and gentle to skin. This process is built-in from the start of product research and development.

Methods: A systematic, iterative approach that includes (1) exposure-based safety assessment of all raw materials for systemic and local effects, which are consistent with established risk assessment paradigms; (2) when needed, testing of finished wipes in and/or clinical studies for skin and eye irritation, contact sensitization, or mechanical effects on skin in comparison to benchmark products that have a long history of wipe safety in marketplace; (3) prospective and randomized in-use studies in babies; and (4) in-market monitoring that is an integral part of the on-going product safety assessment.

Results: Individual approaches and/or their combination show mildness of the test wipes. Further, in-market monitoring is testimony to baby skin compatibility of baby wipes.

Conclusion: The approaches that have been developed demonstrate the skin compatibility and/or benefits of baby wipes relative to other modes of cleaning if needed. This article describes our safety assurance program, exposure-based safety assessment, and the range of robust testing strategies and approaches that can be employed to assure the safety of baby wipes.
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http://dx.doi.org/10.1177/2333794X221105261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9210097PMC
June 2022

The Identification of ECG Signals Using Wavelet Transform and WOA-PNN.

Sensors (Basel) 2022 Jun 8;22(12). Epub 2022 Jun 8.

Department of Electronic, Electrical, and Systems Engineering, School of Engineering, University of Birmingham, Birmingham B15 2TT, UK.

Electrocardiogram (ECG) signal identification technology is rapidly replacing traditional fingerprint, face, iris and other recognition technologies, avoiding the vulnerability of traditional recognition technologies. This paper proposes an ECG signal identification method based on the wavelet transform algorithm and the probabilistic neural network by whale optimization algorithm (WOA-PNN). Firstly, Q, R and S waves are detected by wavelet transform, and the P and T waves are detected by local windowed wavelet transform. The characteristic values are constructed by the detected time points, and the ECG data dimension is smaller than that of the non-reference detection. Secondly, combined with the probabilistic neural network, the mean impact value algorithm is used to screen the characteristic values, the characteristic values with low influence are eliminated, and the input and complexity of the model are simplified. Finally, a WOA-PNN combined classification method is proposed to intelligently optimize the hyper parameters in the probabilistic neural network algorithm to improve the model accuracy. According to the simulation verification on three databases, ECG-ID, MIT-BIH Normal Sinus Rhythm and MIT-BIH Arrhythmia, the identification accuracy of a single ECG cycle is 96.97%, and the identification accuracy of three ECG cycles is 99.43%.
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http://dx.doi.org/10.3390/s22124343DOI Listing
June 2022

Vegetation Responses to Climate Change and Anthropogenic Activity in China, 1982 to 2018.

Int J Environ Res Public Health 2022 Jun 16;19(12). Epub 2022 Jun 16.

College of Geography and Environmental Science, Henan University, Kaifeng 475004, China.

Climate change and human activities significantly affect vegetation growth in terrestrial ecosystems. Here, data reconstruction was performed to obtain a time series of the normalized difference vegetation index (NDVI) for China (1982-2018) based on Savitzky-Golay filtered GIMMS NDVI3g and MOD13A2 datasets. Combining surface temperature and precipitation observations from more than 2000 meteorological stations in China, Theil-Sen trend analysis, Mann-Kendall significance tests, Pearson correlation analysis, and residual trend analysis were used to quantitatively analyze the long-term trends of vegetation changes and their sources of uncertainty. Significant spatial and temporal heterogeneity was observed in vegetation changes in the study area. From 1982 to 2018, the vegetation showed a gradually increasing trend, at a rate of 0.5%·10 a, significantly improving (37.15%, < 0.05) more than the significant degradation (7.46%, < 0.05). Broadleaf (0.66) and coniferous forests (0.62) had higher NDVI, and farmland had the fastest rate of increase (1.02%/10 a). Temperature significantly affected the vegetation growth in spring (R > 0; < 0.05); however, the increase in summer temperatures significantly inhibited (R < 0; < 0.05) the growth in North China (R = -0.379) and the Qinghai-Tibetan Plateau (R = -0.051). Climate change has highly promoted the growth of vegetation in the plain region of the Changjiang (Yangtze) River (3.24%), Northwest China (1.07%). Affected by human activities only, 49.89% of the vegetation showed an increasing trend, of which 22.91% increased significantly ( < 0.05) and 9.97% decreased significantly ( < 0.05). Emergency mitigation actions are required in Northeast China, Xinjiang, Northwest China, and the Qinghai-Tibetan Plateau. Therefore, monitoring vegetation changes is important for ecological environment construction and promoting regional ecological protection.
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http://dx.doi.org/10.3390/ijerph19127391DOI Listing
June 2022

Analysis of Physiological and Transcriptomic Differences between a Premature Senescence Mutant () and Its Wild-Type in Common Wheat ( L.).

Biology (Basel) 2022 Jun 12;11(6). Epub 2022 Jun 12.

State Key Laboratory of Sustainable Dryland Agriculture, College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, China.

Premature leaf senescence has a profound influence on crop yield and quality. Here, a stable premature senescence mutant () was obtained from the common wheat ( L.) cultivar Chang 6878 by mutagenesis with ethyl methanesulfonate. The differences between the mutant and its wild-type (WT) were analyzed in terms of yield characteristics, photosynthetic fluorescence indices, and senescence-related physiological parameters. RNA sequencing was used to reveal gene expression differences between and WT. The results showed that the yield of was considerably lower than that of WT. The net photosynthetic rate, transpiration rate, maximum quantum yield, non-photochemical quenching coefficient, photosynthetic electron transport rate, soluble protein, peroxidase activity, and catalase activity all remarkably decreased in flag leaves of , whereas malondialdehyde content distinctively increased compared with those of WT. The analysis of differentially expressed genes indicated blockade of chlorophyll and carotenoid biosynthesis, accelerated degradation of chlorophyll, and diminished photosynthetic capacity in mutant leaves; brassinolide might facilitate chlorophyll breakdown and consequently accelerate leaf senescence. genes positively regulated the senescence process. Compared with genes, expression of and genes was induced earlier in the mutant possibly due to increased levels of reactive oxygen species and plant hormones (e.g., brassinolide, salicylic acid, and jasmonic acid), thereby accelerating leaf senescence. Furthermore, the antioxidant system played a role in minimizing oxidative damage in the mutant. These results provides novel insight into the molecular mechanisms of premature leaf senescence in crops.
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http://dx.doi.org/10.3390/biology11060904DOI Listing
June 2022

MicroRNA-23a-3p promotes macrophage M1 polarization and aggravates lipopolysaccharide-induced acute lung injury by regulating PLK1/STAT1/STAT3 signalling.

Int J Exp Pathol 2022 Jun 23. Epub 2022 Jun 23.

Department of Emergency, Tiantai Hospital of Hangzhou Medical College, Tiantai, China.

Macrophage polarization is an important effectorprocess in acute lung injury (ALI) induced by sepsis. MicroRNAs (miRNAs) have emerged as important players in regulating ALI process. Here, we showed that elevated microRNA-23a-3p (miR-23a-3p) promoted LPS-induced macrophage polarization and ALI in mice, while inhibition of miR-23a-3p led to reduced macrophage response and ameliorated ALI inflammation. Mechanically, miR-23a-3p regulated macrophage M1 polarization through targeting polo-like kinase 1 (PLK1). PLK1 was downregulated in LPS-treated macrophages and ALI mouse lung tissues. Knockdown of PLK1 increased macrophage M1 polarization through promoting STAT1/STAT3 activation, while overexpression of PLK1 reduced macrophage immune response. Collectively, our results reveal a key miRNA regulon that regulates macrophage polarization for LPS-induced immune response.
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http://dx.doi.org/10.1111/iep.12445DOI Listing
June 2022

Association of clinic setting with quality indicator performance in systemic lupus erythematosus: a cross-sectional study.

Arthritis Res Ther 2022 Jun 22;24(1):150. Epub 2022 Jun 22.

Monash University, Melbourne, Australia.

Background: Healthcare quality for systemic lupus erythematosus (SLE) is a modifiable target for improving patient outcomes. We aimed to assess the quality of care processes in different clinic settings, comparing a subspecialty lupus clinic with hospital-based and private general rheumatology clinics.

Methods: Patients with SLE (n = 258) were recruited in 2016 from a subspecialty lupus clinic (n = 147), two hospital general rheumatology clinics (n = 56) and two private rheumatology clinics (n = 55). Data were collected from medical records and patient questionnaires. Quality of care was assessed using 31 validated SLE quality indicators (QI) encompassing diagnostic work-up, disease and comorbidity assessments, drug monitoring, preventative care and reproductive health. Per-QI performance was measured as a percentage of patients that met the QI relative to the number of patients eligible. Per-patient QI performance was calculated as a percentage of QIs met relative to the number of eligible QIs for each patient. Per-QI and per-patient QI performance were compared between the three clinic settings, and multiple regression performed to adjust for sociodemographic, disease and healthcare factors.

Results: Per-QI performance was generally high across all clinic settings for diagnostic work-up, comorbidity assessment, lupus nephritis, drug monitoring, prednisolone taper, osteoporosis and pregnancy care. Median [IQR] per-patient performance on eligible QIs was higher in the subspeciality lupus clinic (66.7% [57.1-74.1]) than the hospital general rheumatology (52.7% [47.5-58.1]) and private rheumatology (50.0% [42.9-60.9]) clinics (p <0.001) and the difference remained significant after multivariable adjustment. The subspecialty lupus clinic recorded higher per-QI performance for documentation of disease activity, disease damage, cardiovascular risk factor and drug toxicity assessments, pre-immunosuppression hepatitis and tuberculosis screening, new medication counselling, vaccinations, sun avoidance education and contraception counselling.

Conclusions: SLE patients managed in a subspecialty lupus clinic recorded higher per-patient QI performance compared to hospital general rheumatology and private rheumatology clinics, in part related to better documentation on certain QIs.
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http://dx.doi.org/10.1186/s13075-022-02823-9DOI Listing
June 2022

[The soluble expression and application of human papillomavirus type 58 (HPV58) major capsid protein L1].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2022 Jun;38(6):553-558

School of Life Science, Zhengzhou University, Zhengzhou 450001, China. *Corresponding author, E-mail:

Objective The major capsid protein L1 of human papillomavirus type 58 (HPV58 L1) was obtained and identified by prokaryotic expression. Methods The recombinant expression strain pE-SUMO-58 L1 (BL21) was induced by IPTG. The recombinant protein SUMO-58 L1 was expressed in E.coli and identified by SDS-PAGE and Western blot analysis. Then the recombinant protein SUMO-58 L1 was purified by Ni-column and the SUMO-tag was removed by ubiquitin-like protease 1 (ULP1) digestion. Subsequently, the bioactivity of recombinant protein HPV58 L1 was verified by hemagglutination assay (HA). BALB/c mice were immunized with HPV58 L1, and the antibody titers in sera of the immunized mice were detected by ELISA. And then the reaction between the immune serum and the HPV58 L1 protein transiently expressed by HEK293T cells was detected by indirect immunofluorescence assay (IFA). Results The soluble expression of the recombinant protein SUMO-58 L1 was identified by SDS-PAGE and Western blot analysis, with yields of soluble protein SUMO-58 L1 being about 50% of total soluble bacterial proteins. The relative molecular mass (M) of SUMO-58 L1 was about 72 000. After Ni-NTA affinity was purified and the SUMO-tag was removed by ULP1 digestion, M of recombinant protein HPV58 L1 reached about 58 000. The recombinant protein HPV58 L1 showed hemagglutination activity similar to that of natural HPV, with hemagglutination value of 1:16. After immunizing BALB/c mice, the titer of immune serum observed was about 1:10 240 by ELISA; and the sera of the immunized mice reacted specifically with HPV58 L1 proteins which were transiently expressed in HEK293T cells by IFA. Conclusion The recombinant protein HPV58 L1 also has hemagglutination activity, which can be successfully obtained from E. coli. The sera of the HPV58 L1 protein immunized mice can be used for immunocytochemical detection of HPV58 L1 protein expressed in eukaryotic cells.
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June 2022

Biomass ash pyrolyzed from municipal sludge and its adsorption performance toward tetracycline: effect of pyrolysis temperature and KOH activation.

Environ Sci Pollut Res Int 2022 Jun 22. Epub 2022 Jun 22.

College of Environmental and Chemical Engineering, Foshan University, Foshan, 528000, China.

Large amount of municipal sludge is difficult to handle; its resource utilization is an effective measure. In this study, the municipal sludge from sewage treatment plant was pyrolyzed without gas protection at different temperatures and potassium hydroxide (KOH) concentrations for activation. The pyrolysis products, named biomass ash, with higher surface area and enriched pore structures could be obtained at the pyrolysis temperature of 773 K. Moreover, the KOH activation for raw municipal sludge could further increase the surface area of the pyrolysis biomass ash. The maximum specific surface area was 44.71 m/g, which was obtained under 2 mol/L KOH activation before pyrolysis at 773 K. And in this situation, the obtained pyrolysis biomass ash as adsorbent showed the maximum adsorption capacity of 50.75 mg/g toward tetracycline (TC). Moreover, the TC adsorption onto pyrolysis biomass ash obtained under various conditions followed the pseudo-second-order kinetic model. Adsorption thermodynamics analysis suggested the TC adsorption onto the pyrolysis biomass ash with no pre-activation was mainly due to the multi-molecule heterogeneous adsorption, while the TC adsorption onto pyrolysis biomass ash pretreated through the activation of KOH followed the monomer adsorption mechanism. This different adsorption mechanism was largely related to the pore structure, polarity, and aromaticity of the adsorbent.
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http://dx.doi.org/10.1007/s11356-022-21366-9DOI Listing
June 2022

Assessment of hepatic arterial hemodynamics with 4D flow MRI: in vitro analysis of motion and spatial resolution related error and in vivo feasibility study in 20 volunteers.

Eur Radiol 2022 Jun 22. Epub 2022 Jun 22.

Laboratory of Clinical Image Processing (LCTI), Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), 900, rue Saint-Denis, Pavillon R, Montreal, QC, H2X 0A9, Canada.

Objectives: To assess the ability of four-dimensional (4D) flow MRI to measure hepatic arterial hemodynamics by determining the effects of spatial resolution and respiratory motion suppression in vitro and its applicability in vivo with comparison to two-dimensional (2D) phase-contrast MRI.

Methods: A dynamic hepatic artery phantom and 20 consecutive volunteers were scanned. The accuracies of Cartesian 4D flow sequences with k-space reordering and navigator gating at four spatial resolutions (0.5- to 1-mm isotropic) and navigator acceptance windows (± 8 to ± 2 mm) and one 2D phase-contrast sequence (0.5-mm in -plane) were assessed in vitro at 3 T. Two sequences centered on gastroduodenal and hepatic artery branches were assessed in vivo for intra - and interobserver agreement and compared to 2D phase-contrast.

Results: In vitro, higher spatial resolution led to a greater decrease in error than narrower navigator window (30.5 to -4.67% vs -6.64 to -4.67% for flow). In vivo, hepatic and gastroduodenal arteries were more often visualized with the higher resolution sequence (90 vs 71%). Despite similar interobserver agreement (κ = 0.660 and 0.704), the higher resolution sequence had lower variability for area (CV = 20.04 vs 30.67%), flow (CV = 34.92 vs 51.99%), and average velocity (CV = 26.47 vs 44.76%). 4D flow had lower differences between inflow and outflow at the hepatic artery bifurcation (11.03 ± 5.05% and 15.69 ± 6.14%) than 2D phase-contrast (28.77 ± 21.01%).

Conclusion: High-resolution 4D flow can assess hepatic artery anatomy and hemodynamics with improved accuracy, greater vessel visibility, better interobserver reliability, and internal consistency.

Key Points: • Motion-suppressed Cartesian four-dimensional (4D) flow MRI with higher spatial resolution provides more accurate measurements even when accepted respiratory motion exceeds voxel size. • 4D flow MRI with higher spatial resolution provides substantial interobserver agreement for visualization of hepatic artery branches. • Lower peak and average velocities and a trend toward better internal consistency were observed with 4D flow MRI as compared to 2D phase-contrast.
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http://dx.doi.org/10.1007/s00330-022-08890-5DOI Listing
June 2022

Modifiable risk factors associated with cardiovascular disease and mortality in China: a PURE substudy.

Eur Heart J 2022 Jun 22. Epub 2022 Jun 22.

The Red Cross hospital of Shenyang, Shenyang, China.

Aims: To examine the incidence of cardiovascular disease (CVD) and mortality in China and in key subpopulations, and to estimate the population-level risks attributable to 12 common modifiable risk factors for each outcome.

Methods And Results: In this prospective cohort of 47 262 middle-aged participants from 115 urban and rural communities in 12 provinces of China, it was examined how CVD incidence and mortality rates varied by sex, by urban-rural area, and by region. In participants without prior CVD, population-attributable fractions (PAFs) for CVD and for death related to 12 common modifiable risk factors were assessed: four metabolic risk factors (hypertension, diabetes, abdominal obesity, and lipids), four behavioural risk factors (tobacco, alcohol, diet quality, and physical activity), education, depression, grip strength, and household air pollution. The mean age of the cohort was 51.1 years. 58.2% were female, 49.2% were from urban areas, and 59.6% were from the eastern region of China. The median follow-up duration was 11.9 years. The CVD was the leading cause of death in China (36%). The rates of CVD and death were 8.35 and 5.33 per 1000 person-years, respectively, with higher rates in men compared with women and in rural compared with urban areas. Death rates were higher in the central and western regions of China compared with the eastern region. The modifiable risk factors studied collectively contributed to 59% of the PAF for CVD and 56% of the PAF for death in China. Metabolic risk factors accounted for the largest proportion of CVD (PAF of 41.7%), and hypertension was the most important risk factor (25.0%), followed by low education (10.2%), high non-high-density lipoprotein cholesterol (7.8%), and abdominal obesity (6.9%). The largest risk factors for death were hypertension (10.8%), low education (10.5%), poor diet (8.3%), tobacco use (7.5%), and household air pollution (6.1%).

Conclusion: Both CVD and mortality are higher in men compared with women, and in rural compared with urban areas. Large reductions in CVD could potentially be achieved by controlling metabolic risk factors and improving education. Lowering mortality rates will require strategies addressing a broader range of risk factors.
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http://dx.doi.org/10.1093/eurheartj/ehac268DOI Listing
June 2022

Immune checkpoint inhibitors-associated cardiotoxicity in immunotherapy trials on gastrointestinal cancer patients.

Chin Med J (Engl) 2022 04 20;135(8):988-990. Epub 2022 Apr 20.

Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

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http://dx.doi.org/10.1097/CM9.0000000000002054DOI Listing
April 2022

Visible-Light-Induced Copper-Catalyzed Asymmetric C(sp)-C(sp)-H Glycosylation: Access to C-Glycopeptides.

Org Lett 2022 Jun 21. Epub 2022 Jun 21.

State Key Laboratory of Applied Organic Chemistry, School of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P.R. China.

Herein, a practical and highly efficient method for visible-light-induced copper-catalyzed -aminoquinoline-directed asymmetric C(sp)-C(sp)-H glycosylation was reported. At the same time, C(sp)-C(sp)-H glycosylation of nondeoxysugars with amino acids to construct C-glycopeptides was achieved. This approach promoted the synthesis of various C-glycopeptides and provided a new model for the synthesis of C-glycoamino acids.
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http://dx.doi.org/10.1021/acs.orglett.2c01501DOI Listing
June 2022

Association between noise exposure and atrial fibrillation: a meta-analysis of cohort studies.

Environ Sci Pollut Res Int 2022 Jun 21. Epub 2022 Jun 21.

Department of Epidemiology and Health Statistics, School of Public Health, Anhui Medical University, No. 81 Meishan Road, Hefei, 230032, Anhui, China.

Noise has become an important environmental risk factor. Some studies have shown that exposure to noise can cause coronary artery disease, high blood pressure, and stroke. At present, the relationship between noise exposure and the risk of atrial fibrillation (AF) is inconsistent. Based on previous studies, we proposed the hypothesis that noise exposure is associated with a higher risk of AF. Eight databases, such as PubMed, Embase, Cochrane Library, and Web of Science, were searched from inception until January 5, 2022. The pooled relative risk (RR) with its 95% confidence interval (CI) was used to estimate the association between AF and highest noise level and per 10 dB (A) increment of noise. According to the size of heterogeneity, the random or fixed effects model was adopted as the pooling method. A total of 5 articles comprising 3,866,986 participants were identified, providing 7 estimates of highest noise level and 6 estimates of per 10 dB (A) increment of noise exposure. For the highest noise level, there was a statistically significant association between noise exposure and the risk of AF (RR = 1.05; 95% CI: 1.02-1.09; I = 44.1%). In addition, we found the risk of AF for per 10 dB (A) increment of noise exposure was 1.01 (95% CI: 1.00-1.02; I = 81.3%). In summary, our study found that noise exposure was associated with a higher risk of AF. More high-quality studies are needed in the future to confirm these conclusions given the limitations of study.
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http://dx.doi.org/10.1007/s11356-022-21456-8DOI Listing
June 2022

Polydatin attenuates chronic alcohol consumption-induced cardiomyopathy through a SIRT6-dependent mechanism.

Food Funct 2022 Jun 21. Epub 2022 Jun 21.

Department of Cardiovascular Surgery, General Hospital of Northern Theater Command, 83 Wenhua Road, Shenyang, Liaoning 110016, P. R. China.

Polydatin has attracted much attention as a potential cardioprotective agent against ischemic heart disease and diabetic cardiomyopathy. However, the effect and mechanism of polydatin supplementation on alcoholic cardiomyopathy (ACM) are still unknown. This study aimed to determine the therapeutic effect of polydatin against ACM and to explore the molecular mechanisms with a focus on SIRT6-AMP-activated protein kinase (AMPK) signaling and mitochondrial function. The ACM model was established by feeding C57/BL6 mice with an ethanol Lieber-DeCarli diet for 12 weeks. The mice received polydatin (20 mg kg) or vehicle treatment. We showed that polydatin treatment not only improved cardiac function but also reduced myocardial fibrosis and dynamin-related protein 1 (Drp-1)-mediated mitochondrial fission, and enhanced PTEN-induced putative kinase 1 (PINK1)-Parkin-dependent mitophagy in alcohol-treated myocardium. Importantly, these beneficial effects were mimicked by SIRT6 overexpression but abolished by the infection of recombinant serotype 9 adeno-associated virus (AAV9) carrying SIRT6-specific small hairpin RNA. Mechanistically, alcohol consumption induced a gradual decrease in the myocardial SIRT6 level, while polydatin effectively activated SIRT6-AMPK signaling and modulated mitochondrial dynamics and mitophagy, thus reducing oxidative stress damage and preserving mitochondrial function. In summary, these data present new information regarding the therapeutic actions of polydatin, suggesting that the activation of SIRT6 signaling may represent a new approach for tackling ACM-related cardiac dysfunction.
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http://dx.doi.org/10.1039/d2fo00966hDOI Listing
June 2022

Serum IMA and LP-PLA2 Levels in Patients with Coronary Heart Disease and Their Correlation with the Degree of Myocardial Ischaemia and Their Diagnostic Value.

Emerg Med Int 2022 11;2022:1698315. Epub 2022 Jun 11.

Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Purpose: To measure serum levels of ischaemia-modified albumin (IMA) and lipoprotein-associated phospholipase A2 (LP-PLA2) in patients with coronary heart disease (CHD) and to analyse their correlation with the degree of myocardial ischaemia and their diagnostic value.

Methods: A sample of 150 patients diagnosed with CHD by coronary angiography in our hospital from March 2019 to September 2021 was taken as the CHD group. The patients were divided into acute myocardial infarction (AMI) group ( = 52), unstable angina pectoris (UAP) group ( = 54), and stable angina pectoris (SAP) group ( = 44) according to the degree of myocardial ischaemia, and then 50 healthy physical examination patients were selected as the health group during the same period. Serum C-reactive protein (CRP), interleukin-6 (IL-6), IMA, and LP-PLA2 levels were measured in each group separately. Multiple ordered logistic regression was used to analyse the factors influencing the degree of myocardial ischaemia in patients with CHD. Pearson correlation was used to analyse the correlation between serum IMA, LP-PLA2 levels and serum CRP, IL-6 levels in CHD patients. The diagnostic value of IMA alone, LP-PLA2 alone, and in combination for CHD was analysed using receiver operating characteristic (ROC) curves.

Results: In terms of serum CRP, IL-6, IMA, and LP-PLA2 levels, the CHD group was higher than the health group, the AMI and UAP groups were higher than the SAP and health groups, and the AMI group was higher than the UAP group ( < 0.05). Multiple ordered logistic regression analysis showed that serum CRP, IL-6, IMA, and LP-PLA2 levels were all independent influences on the degree of myocardial ischaemia in patients with CHD ( < 0.05). Pearson correlation analysis showed a positive correlation between serum IMA, LP-PLA2 levels and serum CRP, IL-6 levels in CHD patients ( < 0.001). The area under curve (AUC) for serum IMA levels to predict myocardial ischaemia in patients with CHD was 0.754 (95% CI: 0.684-0.825), with a sensitivity of 61.3% and specificity of 84.0% when the best cut-off value was 0.453; the AUC for serum LP-PLA2 levels to predict myocardial ischaemia in patients with CHD was 0.747 (95% CI: 0.681-0.813), with a sensitivity of 62.0% and specificity of 82.0% when the optimal cut-off value was 0.440; and the AUC of IMA + LP-PLA2 for predicting myocardial ischaemia in patients with CHD was 0.892 (95% CI: 0.847-0.938), with a sensitivity of 86.7% and specificity of 80.0% when the optimal cut-off value was 0.667. The specificity was 80.0%.

Conclusions: Serum IMA and LP-PLA2 levels are elevated in patients with CHD. Serum IMA and LP-PLA2 levels are closely related to the degree of myocardial ischaemia and its inflammatory level, and the combination of IMA + LP-PLA2 can improve the diagnosis efficacy of myocardial ischaemia in CHD patients.
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http://dx.doi.org/10.1155/2022/1698315DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206582PMC
June 2022

Estrogen Receptor- And Progesterone Receptor-Positive Thresholds in Predicting the Recurrence of Early Low-Risk Endometrial Cancer.

Clin Med Insights Oncol 2022 15;16:11795549221103200. Epub 2022 Jun 15.

Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Estrogen receptors (ER) and progesterone receptors (PR) have important prognostic value in endometrial cancer, but there is no recognized positive immunohistochemical threshold for predicting the recurrence of early low-risk endometrial cancer. The purpose of this study was to clarify the optimal positive thresholds of the immunohistochemical parameters the ER and PR in early low-risk endometrial cancer.

Methods: A total of 332 patients with stage IA endometrial cancer were enrolled from the First Affiliated Hospital of Chongqing Medical University and People's Hospital of Sha ping ba District in Chongqing between January 2013 and December 2018. First, univariate and multivariate Cox regression analyses were used to analyze the correlation between various clinical factors and the prognosis of early low-risk endometrial cancer. Then, the receiver operating characteristic curve (ROC curve) and Youden index were used to determine the positive thresholds of ER and PR.

Results: The positive thresholds of ER and PR for predicting the recurrence of early low-risk endometrial cancer were 12% and 8%, respectively. Multivariate analysis showed that ER ( = 0.004), PR ( = 0.026), and p53 ( = 0.021) were risk factors for the prognosis of patients with early low-risk endometrial cancer. The recurrence-free survival and the overall survival in the low ER group and PR group were much lower than those in the high ER group and PR group ( < 0.001 of all).

Conclusions: ER and PR positive thresholds of 12% and 8%, respectively, are the most suitable for predicting the recurrence of early low-risk endometrial cancer.
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http://dx.doi.org/10.1177/11795549221103200DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203725PMC
June 2022

Appropriateness and Associated Factors of Stress Ulcer Prophylaxis for Surgical Inpatients of Orthopedics Department in a Tertiary Hospital: A Cross-Sectional Study.

Front Pharmacol 2022 2;13:881063. Epub 2022 Jun 2.

Department of Pharmacy, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

Stress ulcer prophylaxis (SUP) prescribed in patients admitted to surgical wards with a low risk of stress-related mucosal disease (SRMD) accounted for a considerable proportion of improper use of proton pump inhibitors (PPIs). This study aimed to analyze the appropriateness of SUP prescribing patterns and identify its associated factors in the orthopedics department of a tertiary hospital in the Northwestern China. In this cross-sectional study, information regarding the demographic and clinical characteristics of 1,200 fracture inpatients who underwent surgical operations from January 2020 to August 2021 were collected from medical records. Established criteria were used to assess the appropriateness of the prescribing pattern for SUP, and the incidence of inappropriate SUP medication was calculated. Logistic regression analyses were used to identify factors associated with inappropriate SUP medication. Approximately, 42.4% of the study population was interpreted as inappropriate prescription of SUP. A total of 397 (33.1%) patients received SUP without a proper indication (overprescription), and the incidence of inappropriate SUP medication was calculated to be 43.11 per 100 patient-days. In addition, 112 (9.3%) inpatients for whom SUP was indicated did not receive SUP (underprescription). PPIs were prescribed in 96.1% of the inpatients who used acid suppression therapy (AST), and intravenous PPIs accounted for 95.3% thereof. In a multivariate logistic regression analysis, age above 65 years and prolonged hospitalization were associated with overprescription of SUP. Increased number of drugs excluding PPIs, the concurrent use of systemic corticosteroids, comorbidity of hypertension, and unemployed or retired status in inpatients were associated with a reduced likelihood of overprescription for SUP. Conversely, prolonged hospitalization, the concurrent use of systemic corticosteroids or anticoagulants, and unemployed status in inpatients were positively associated with underprescription of SUP. There was a high prevalence of inappropriate SUP prescription among noncritically ill inpatients of fracture who underwent surgical operations. We delineated the associated factors with inappropriate SUP medication, which indicated that more information was required for clinicians about rationality and efficiency of their prescribing practices. Effective intervention strategies should be executed by clinical pharmacists to reduce improper SUP medication.
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http://dx.doi.org/10.3389/fphar.2022.881063DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203048PMC
June 2022

Efficacy of the Oral Administration of Maltodextrin Fructose Before Major Abdominal Surgery: A Prospective, Multicenter Clinical Study.

World J Surg 2022 Jun 19. Epub 2022 Jun 19.

Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.

Background: To study the efficacy of the oral administration of maltodextrin and fructose before major abdominal surgery (MAS).

Methods: This prospective, multicenter, parallel-controlled, double-blind study included patients aged 45-70 years who underwent elective gastrectomy, colorectal resection, or duodenopancreatectomy. The intervention group (IG) was given 800 mL and 400 mL of a maltodextrin and fructose beverage at 10 h and 2 h before MAS, respectively, and the control group (CG) received water under the same experimental conditions. The primary endpoint was insulin resistance index (IRI), and the secondary endpoints were fasting blood glucose, fasting insulin, insulin secretion index, insulin sensitivity index, intraoperative blood glucose, subjective comfort score, and clinical outcome indicators.

Results: A total of 240 cases were screened, of which 231 cases were randomly divided into two groups: 114 in the IG and 117 in the CG. No time-treatment effect was detected for any endpoint. The IRI and fasting insulin were significantly lower in the IG than CG after MAS (p = 0.02 & P = 0.03). The scores for anxiety, appetite, and nausea were significantly lower in the IG than CG at 1 h before MAS. Compared with baseline, the scores for appetite and nausea decreased in the IG but increased in the CG.

Conclusion: The oral administration of maltodextrin and fructose before MAS can improve preoperative subjective well-being and reduce postoperative insulin resistance without increasing the risk of gastrointestinal discomfort.
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http://dx.doi.org/10.1007/s00268-022-06455-7DOI Listing
June 2022

Polyisocyanide hydrogels with tunable nonlinear elasticity mediate liver carcinoma cell functional response.

Acta Biomater 2022 Jun 16. Epub 2022 Jun 16.

Key Laboratory of Hebei Province for Molecular Biophysics, Institute of Biophysics, School of Science, Hebei University of Technology, Tianjin 300401, PR China. Electronic address:

Hepatocellular carcinoma development is closely related to the changes in tissue mechanics induced by excess collagen deposition and crosslinking, which leads to liver fibrosis and malignant progression. The role of matrix stiffness has been widely assessed using various linearly elastic materials. However, the liver, like many soft tissues, also exhibits nonlinear elasticity by strain-stiffening, allowing cells to mechanically interact with their micromilieus which has attracted much attention in cellular processes recently. Here, we use a biomimetic hydrogel grafting of GRGDS peptide with tunable nonlinear mechanical properties, polyisocyanides (PIC), to investigate the influence of strain-stiffening on HepG2 liver cancer cell behavior by tuning PIC polymer length. Compared to short PIC polymer with lower critical stress, PIC hydrogels composed of long polymer with higher critical stress promote the motility and invasiveness of HepG2 cells, and induce more actin stress fibers and higher expression level of mechanotransducer YAP and its nuclear translocation. Strikingly, the expression of calcium-activated potassium channel KCa3.1, an important biomarker in hepatocellular carcinoma, is also affected by the mechanical property of PIC hydrogels. It was also shown that downregulating the KCa3.1 channel can be achieved by inhibiting the formation of actin fibers. Our findings imply that the strain-stiffening property of PIC hydrogels affects the expression of KCa3.1 potassium channel via mediating cytoskeletal stress fiber formation, and ultimately influences the liver carcinoma cell functional response. STATEMENT OF SIGNIFICANCE: The effect of nonlinear elasticity by strain-stiffening, is assessed in HepG2 liver cancer cell behavior by using a biomimetic hydrogel with tunable mechanical properties, polyisocyanides (PIC). PIC gels with higher critical stress promote the motility and invasiveness of HepG2 cells and induce upregulated expression levels of KCa3.1 potassium channel and YAP, but which can be suppressed by inhibiting the formation of actin fibers. Our findings imply that the strain-stiffening property of PIC gels influences the expression of KCa3.1 potassium channel via mediating cytoskeletal stress fiber formation and, ultimately affects the liver carcinoma cell functional response.
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http://dx.doi.org/10.1016/j.actbio.2022.06.022DOI Listing
June 2022

[Comparative study of microscope assisted minimally invasive anterior fusion and mobile microendoscopic discectomy assisted fusion for lumbar degenerative diseases].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Jun;36(6):672-680

Department of Minimally Invasive Spine Surgery, Tianjin Hospital, Tianjin, 300211, P. R. China.

Objective: To investigate the effectiveness of microscope assisted anterior lumbar discectomy and fusion (ALDF) and mobile microendoscopic discectomy assisted lumbar interbody fusion (MMED-LIF) for lumbar degenerative diseases.

Methods: A clinical data of 163 patients with lumbar degenerative diseases who met the criteria between January 2018 and December 2020 was retrospectively analyzed. Fifty-three cases were treated with microscope assisted ALDF (ALDF group) and 110 cases with MMED-LIF (MMED-LIF group). There was no significant difference between the two groups in terms of gender, age, disease type, surgical segments, preoperative visual analogue scale (VAS) scores of low back pain and leg pain, Oswestry disability index (ODI), intervertebral space height, lordosis angle, and spondylolisthesis rate of the patients with lumbar spondylolisthesis ( >0.05). The operation time, intraoperative blood loss, and hospital stay of the two groups were recorded. The effectiveness was evaluated by VAS scores of low back pain and leg pain and ODI. Postoperative lumbar X-ray films were taken to observe the position of Cage and measure the intervertebral space height, lordosis angle, and spondylolisthesis rate of the patients with lumbar spondylolisthesis.

Results: The operations were successfully completed in both groups. The operation time, intraoperative blood loss, and hospital stay in ALDF group were less than those in MMED-LIF group ( <0.05). The patients in both groups were followed up 12-36 months, with an average of 24 months. The VAS scores of low back pain and leg pain and ODI after operation were lower than those before operation in the two groups, and showed a continuous downward trend, with significant differences between different time points ( <0.05). There were significant differences between two groups in VAS score of low back pain and ODI ( <0.05) and no significant difference in VAS score of leg pain ( >0.05) at each time point. The improvement rates of VAS score of low back pain and ODI in ALDF group were significantly higher than those in MMED-LIF group ( =7.187, =0.000; =2.716, =0.007), but there was no significant difference in the improvement rate of VAS score of leg pain ( =0.556, =0.579). The postoperative lumbar X-ray films showed the significant recovery of the intervertebral space height, lordosis angle, and spondylolisthesis rate at 2 days after operation when compared with preoperation ( <0.05), and the improvements were maintained until last follow-up ( >0.05). The improvement rates of intervertebral space height and lordosis angle in ALDF group were significantly higher than those in MMED-LIF group ( <0.05). There was no significant difference in the reduction rate of spondylolisthesis between the two groups ( =1.396, =0.167). During follow-up, there was no loosening or breakage of the implant and no displacement or sinking of the Cage.

Conclusion: Under appropriate indications, microscope assisted ALDF and MMED-LIF both can achieve good results for lumbar degenerative diseases. Microscope assisted ALDF was superior to MMED-LIF in the improvement of low back pain and function and the recovery of intervertebral space height and lordosis angle.
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http://dx.doi.org/10.7507/1002-1892.202202039DOI Listing
June 2022

Identification of an inflammatory response signature associated with prognostic stratification and drug sensitivity in lung adenocarcinoma.

Sci Rep 2022 Jun 16;12(1):10110. Epub 2022 Jun 16.

Department of Thoracic Surgery, Renmin Hospital of Wuhan University, No.238 Jiefang Road, Wuchang District, Wuhan, 430060, China.

Increasing evidence has confirmed the close connection between inflammatory response and tumorigenesis. However, the relationship between inflammatory response genes (IRGs) and the prognosis of lung adenocarcinoma (LUAD) as well as the response to drug therapy remains poorly investigated. Here, we comprehensively analyzed IRGs RNA expression profiling and clinical features of over 2000 LUAD patients from 12 public datasets. The Cox regression method and LASSO analysis were combined to develop a novel IRG signature for risk stratification and drug efficacy prediction in LUAD patients. Enriched pathways, tumor microenvironment (TME), genomic and somatic mutation landscape in different subgroups were evaluated and compared with each other. This established IRG signature including 11 IRGs (ADM, GPC3, IL7R, NMI, NMURI, PSEN1, PTPRE, PVR, SEMA4D, SERPINE1, SPHK1), could well categorize patients into significantly different prognostic subgroups, and have better predictive in independently assessing survival as compared to a single clinical factor. High IRG scores (IRGS) patients might benefit more from immunotherapy and chemotherapy. Comprehensive analysis uncovered significant differences in enriched pathways, TME, genomic and somatic mutation landscape between the two subgroups. Additionally, integrating the IRGS and TNM stage, a reliable prognostic nomogram was developed to optimize survival prediction, and validated in an independent external dataset for clinical application. Take together, the proposed IRG signature in this study is a promising biomarker for risk stratification and drug efficacy prediction in LUAD patients. This study may be meaningful for explaining the responses of clinical therapeutic drugs and providing new strategies for administrating sufferer of LUAD.
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http://dx.doi.org/10.1038/s41598-022-14323-6DOI Listing
June 2022

Long-term safety of faecal microbiota transplantation for gastrointestinal diseases in China.

Lancet Gastroenterol Hepatol 2022 Jun 13. Epub 2022 Jun 13.

Department of Colorectal Disease, Intestinal Microenvironment Treatment Center, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China; Clinical Research Center for Digestive Diseases of Tongji University, Shanghai, China; Shanghai Institution of Gut Microbiota Research and Engineering Development, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/S2468-1253(22)00170-4DOI Listing
June 2022

High expression of predict poor prognosis of colorectal cancer and influence the immune infiltrating level.

Transl Cancer Res 2022 May;11(5):981-992

Department of Hematology and Oncology, Hangzhou Normal University Affiliated Hospital, Hangzhou, China.

Background: Colorectal cancer (CRC) is the third most common malignancy. Immunoinvasion of tumor microenvironment was positively correlated with overall cancer survival. is a little-reported gene in tumors, and its mechanism of action remains unclear. Our study used The Cancer Genome Atlas (TCGA) data to evaluate the role of in CRC.

Methods: Software R3.6.3 analyzed the expression of in CRC. We assessed the impact of on the survival of CRC patients through a survival module. The CRC data set was then downloaded from TCGA. Logistic regression was used to analyze the correlation between clinical information and expression. Cox regression analysis showed that clinicopathological characteristics of TCGA patients were correlated with overall survival. In addition, we used ssGSEA to explore the correlation between and tumor immune invasion.

Results: High expression of is significantly correlated with advanced pathological stage and poor overall survival. In multivariate analysis, the up-regulated expression, high tumor stage, and distant metastasis are independent prognostic factors of poor prognosis. Moreover, a negative correlation between increased expression and immune infiltrating T cells and neutrophils cells was established.

Conclusions: High expression of was associated with poor prognosis of CRC and affected the proportion of immune cells such as T cells, neutrophils, and NK cells. These results suggest that can be used as a potential biomarker to evaluate the prognosis and level of immune invasion in CRC.
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http://dx.doi.org/10.21037/tcr-21-2674DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189158PMC
May 2022

Synthesis of enol phosphates directly from ketones a modified one-pot Perkow reaction.

RSC Adv 2022 May 17;12(23):14844-14848. Epub 2022 May 17.

State Key Laboratory of NBC Protection for Civilian Beijing 102205 P. R. China

A modified Perkow reaction, named Perkow-Shi reaction, was developed based on the one-pot α-tosyloxylation of ketones following by addition of P(iii)-reagents and 4 Å molecular sieves. Diversity of enol phosphates, as well as enol phosphonates, enol phosphinates, and enol phosphoramidates were synthesized in high yields directly from the ubiquitously available ketones instead of the unfavourable α-chloroketones under a mild and environmental friendly condition.
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http://dx.doi.org/10.1039/d2ra02340gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9112406PMC
May 2022

HSA-MnO-I Combined Imaging and Treatment of Anaplastic Thyroid Carcinoma.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221106557

Department of Nuclear Medicine, 117865Tianjin Medical University General Hospital, Tianjin, P. R. China.

Compelling evidence suggests that nanoparticles (NPs) play a crucial role in cancer therapy. NPs templated with human serum albumin (HSA) has good retention in tumors. Manganese dioxide (MnO) has been used to enhance the effect of radiotherapy. In this study, synthesized NPs using HSA-MnO labeled I to perform both imaging and therapy for anaplastic thyroid carcinoma (ATC). HSA-MnO was synthesized via HSA using a simple biomineralization method, and then labeled with NaI by the chloramine T method. The cytotoxicity and biosafety of HSA-MnO were evaluated by the MTT test. The proliferation-inhibiting effect of HSA-MnO-I was evaluated in papillary thyroid cancer cell lines (K1, BCPAP, and KTC) and anaplastic thyroid carcinoma cell lines (Cal62, THJ16T, and ARO). For further translational application in medicine, we established a model of transplantable subcutaneously tumors in BALB\c-nu mice to assess the anti-tumor effect of HSA-MnO-I. The imaging effects of NPs were evaluated by MRI and SPECT/CT. The MTT test proved that the HSA-MnO had low toxicity. HSA-MnO-I significantly inhibited the proliferation of PTC and ATC cell lines. In addition, the results unveiled that HSA-MnO-I exhibited dual-modality MR/SPECT imaging for thyroid cancer visualization. In particular, HSA-MnO-I had an enhanced T1 signal in MR. Using SPECT/CT, we observed that HSA-MnO-I had good retention in tumor tissue, which was helpful for the diagnosis and treatment of tumor. assays indicated that the NPs led to a reduction in radioresistance in the tumor hypoxic microenvironment. The nanomaterial had a simple synthesis method, good water solubility and biosafety, and good retention in tumor tissue. Hence, it could be used for SPECT/CT and MR dual mode imaging and therapy with radioiodine of tumor cells. The experimental results provided a feasible solution for combining radiotherapy and dual-model imaging by NPs for cancer diagnosis and treatment.
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http://dx.doi.org/10.1177/15330338221106557DOI Listing
June 2022

Anti-HIV Tigliane-Type Diterpenoids from the Aerial Parts of .

J Nat Prod 2022 Jun 14;85(6):1658-1664. Epub 2022 Jun 14.

School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang110016, People's Republic of China.

Tigliane-type diterpenoids have attracted much attention in drug discovery since they have been reported to exhibit remarkable biological effects, such as tumor-promoting, antineoplastic, and anti-HIV activities. In continuing our efforts to discover novel biologically important diterpenoids from species, was investigated phytochemically for the first time. As a result, four new (-) and one known () tigliane-type diterpenoid were isolated, and their structures were elucidated by spectroscopic data analysis. Tiglianes (-) showed potent anti-HIV activity against HIV-1 infection of MT4 lymphocytes with IC values of 1.1-65.4 nM.
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http://dx.doi.org/10.1021/acs.jnatprod.1c01195DOI Listing
June 2022
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