Publications by authors named "Ning Fan"

74 Publications

Lentiviral vector-mediated expression of C3 transferase attenuates retinal ischemia and reperfusion injury in rats.

Life Sci 2021 May 22;272:119269. Epub 2021 Feb 22.

Xiamen Eye Center, Xiamen University, Xiamen 361006, China; Department of Ophthalmology, the 2nd Clinical Medical College, Jinan University, Shenzhen 518020, China. Electronic address:

Aims: Our previous study showed that intravitreal delivery of self-complementary AAV2 (scAAV2)-mediated exoenzyme C3 transferase (C3) can attenuate retinal ischemia/reperfusion (I/R) injury. The current study investigated the neuroprotective effects of lentivirus (LV)-mediated C3 transgene expression on rat retinal I/R injury.

Main Methods: The LV encoding C3 and green fluorescent protein (GFP) together (LV-C3-GFP) or GFP only (LV-GFP) was intravitreally injected to SPRAGUE-DAWLEY rats. On day 5 post-intravitreal injection, eyes were evaluated by slit-lamp examination. The GFP expression on retina was confirmed by in vivo and ex vivo assessments. RhoA GTPase expression in retina was examined by western blot. Retinal I/R injury was generated by transiently increasing intraocular pressure (110 mmHg, 90 min). Eyes were then enucleated, and retinas processed for morphological analysis and TdT-dUTP terminal nick-end labeling (TUNEL) assay.

Key Findings: No obvious inflammatory reactions or surgical complications were observed after intravitreal injection of LV vectors. There was a significant decrease of total RhoA GTPase level in the retina treated with LV-C3-GFP. Compared to the blank control group, LV-C3-GFP and LV-GFP did not affect the retinal thickness, cell density in ganglion cell layer (GCL), or numbers of apoptotic cells in retinal flat-mounts. In the LV-GFP-treated retinas, I/R decreased the retinal thickness and GCL cell density and increased apoptotic retinal cell numbers. LV-C3-GFP significantly protected against all these degenerative effects of I/R.

Significance: This study indicated that LV-mediated C3 transgene expression exhibits neuroprotective effects on the retinal I/R injury and holds potential as a novel neuroprotective approach targeting certain retinopathies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.lfs.2021.119269DOI Listing
May 2021

Mutations Were Associated with the Early Recurrence of Patients with HCC Underwent Hepatectomy.

Pharmgenomics Pers Med 2021 16;14:269-278. Epub 2021 Feb 16.

Liver Disease Center, The Affiliated Hospital of Qingdao University, Qingdao, 266003, People's Republic of China.

Purpose: To explore the value of Tuberous sclerosis complex 2 () mutations in evaluating the early recurrence of hepatocellular carcinoma (HCC) patients underwent hepatectomy.

Patients And Methods: A total of 183 HCC patients were enrolled. Next-generation sequencing was performed on tumor tissues to analyze genomic alterations, tumor mutational burden and variant allele fraction (VAF). The associations between mutations and recurrence rate within 1 year, RFS and OS after hepatectomy were analyzed.

Results: Our results showed that mutation frequency in HCC was 12.6%. Compared to patients without mutation, the proportion of microvascular invasion (MVI) and Edmondson grade III-IV was significantly higher in patients with a mutation (p<0.05). The VAF of mutated was higher in patients with maximum diameter of tumor >5cm or MVI than that of other patients (p<0.05). The frequency of mutation was significantly higher in patients with a mutation than those without mutation (p=0.003). Follow-up analysis showed that patients with a mutation had significantly higher recurrence rate within 1 year (=0.015) and poorer median recurrence-free survival (RFS) (=0.010) than patients without mutation. TSC2 mutations did not significantly affect overall survival of patients (=0.480). The multivariate analysis results showed that the Barcelona Clinic Liver Cancer (BCLC) B-C stage, mutations and preoperative serum alpha-fetoprotein level ≥400μg/L were independently associated with recurrence within 1 year after hepatectomy (HR=8.628, 95% CI: 3.836-19.405, =0.000; HR=3.885, 95% CI: 1.295-11.653, =0.015; HR=2.327, 95% CI: 1.018-5.323, =0.045; respectively), and poorer RFS after hepatectomy (HR=3.070, 95% CI: 1.971-4.783, =0.000; HR=1.861, 95% CI: 1.061-3.267, =0.030; HR=1.715, 95% CI: 1.093-2.693, =0.019; respectively).

Conclusion: mutations were significantly associated with MVI in liver para-carcinoma tissue and Edmondson grade III-IV in patients with HCC and were independently associated with recurrence within 1 year and poorer RFS after hepatectomy. The TSC2 mutation may be a potential predictor for early recurrence in HCC patients underwent hepatectomy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/PGPM.S294307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7896791PMC
February 2021

Clinically significant genomic alterations in the Chinese and Western patients with intrahepatic cholangiocarcinoma.

BMC Cancer 2021 Feb 12;21(1):152. Epub 2021 Feb 12.

Organ Transplant Center, The Affiliated Hospital of Qingdao University, Haier Road No. 59, Qingdao, 266000, Laoshan District, China.

Background: The goal of this study is to disclose the clinically significant genomic alterations in the Chinese and Western patients with intrahepatic cholangiocarcinoma.

Methods: A total of 86 Chinese patients were enrolled in this study. A panel of 579 pan-cancer genes was sequenced for the qualified samples from these patients. Driver genes, actionability, and tumor mutational burden were inferred and compared to a cohort of Western patients.

Results: Totally, 36 and 12 driver genes were identified in the Chinese and Western cohorts, respectively. Of them, seven driver genes (IDH1, KRAS, TP53, BAP1, PBRM1, ARID1A, and NRAS) were shared by the two cohorts. Four driver genes (SPTA1, ARID2, TP53, and GATA1) were found significantly correlated with the tumor mutational burden. For both cohorts, half of the patients had actionable mutations. The two cohorts shared the most actionable genes but differed much in their frequency. Though KRAS mutations were at the first and second actionable rank respectively for the Chinese and Western populations, they were still at a relatively low level of actionable evidence.

Conclusions: The study on the clinical significance of genomic alterations directs the future development of precision medicine for intrahepatic cholangiocarcinoma treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12885-021-07792-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879680PMC
February 2021

What is the impact of knee morphology on posterior cruciate ligament avulsion fracture in men and women: a case control study.

BMC Musculoskelet Disord 2021 Jan 21;22(1):100. Epub 2021 Jan 21.

Department of Orthopedics, Beijing Shunyi District Hospital, Beijing, China.

Background: Several studies on the relationship between morphological parameters and traumatic diseases of the knee have already been conducted. However, few studies focused on the association between knee morphology and posterior cruciate ligament (PCL) avulsion fracture in adults. The objective of this study was to evaluate the impact of knee morphology on PCL avulsion fracture.

Methods: 76 patients (comprised 40 men and 36 women) with PCL avulsion fracture and 76 age- and sex-matched controls without PCL avulsion fracture were studied from 2012 to 2020. MRI measurements of the knee were acquired in the sagittal, coronal, and axial planes. The assessed measurements including intercondylar notch width index, coronal tibial slope, and medial/lateral posterior tibial slopes were compared between men and women, and between case and control groups respectively using independent sample t-tests. In addition, binary logistic regression analyses were used to identify independent risk factors of PCL avulsion fracture.

Results: Except notch width index (coronal) (p = 0.003) in the case groups, there was no statistical difference in the assessed measurements including notch width index (axial), coronal tibial slope, medial posterior tibial slope, and lateral posterior tibial slope between men and women in the case and control groups (p > 0.05). When female patients were analyzed, the notch width index (coronal) was significantly smaller (p = 0.0004), the medial posterior tibial slope (p = 0.018) and the lateral posterior tibial slope (p = 0.033) were significantly higher in the case group. The binary logistic regression analysis showed that the notch width index (coronal) (B = -0.347, OR = 0.707, p = 0.003) was found to be an independent factor of PCL avulsion fracture. However, none of the assessed measurements was found to have a statistical difference between the case and control groups in men (p > 0.05).

Conclusions: Notch width index (coronal), medial posterior tibial slope, and lateral posterior tibial slope were found to affect PCL avulsion fracture in women, but no such measurements affected the PCL avulsion fracture in men. Furthermore, a smaller notch width index (coronal) in women was found to be a risk factor in PCL avulsion fracture.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12891-021-03984-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7819342PMC
January 2021

Liquid Transport in Fibrillar Channels of Ion-Associated Cellular Nanowood Foams.

ACS Appl Mater Interfaces 2020 Dec 15;12(52):58212-58222. Epub 2020 Dec 15.

Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environment Science & Engineering, Nanjing University of Information Science & Technology, Nanjing 210044, China.

A mechanical disintegration of waste wood biomass and freeze-induced assembly of colloidal nanowood were effectively deployed to explore ion-associated cellular foams (NWFs) with unidirectional channels. Under the assistance of inorganic ions, the as-fabricated foams were significantly enhanced in physical stability, compressive strength, flame retardancy, and thermal barrier, accounting for the tuning effects of pores and channels, surface charges, and microphase interaction by ion effects and freeze orientation. As a result, the vascular-like ion-doped channels benefited from quick capillary liquid transport. Under 1 sun illumination, NWF-V as a 3-D evaporator exhibited a high evaporation rate of 1.50 kg m h and a conversion efficiency of up to 88.9% for seawater desalination. Dramatically, an average of 12.5 kg m of fresh water could be generated on each sunny day by outdoor NWFs for durability beyond 15 days. Under the drive of fuel combustion, an efficient conveying of ethanol or pump oil could be at rates of 0.44 and 0.26 mL min, respectively. Moreover, combustion flame with variable color was generated according to the doping cations in NWFs. Therefore, sustainable, green, facile, and multifunctional wood-based cellular foams could be tailored, scaled-up, and applied as color flame burners or desalination evaporators under combustion or solar drive in the energy and environment fields.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c17034DOI Listing
December 2020

Compositional and drug-resistance profiling of pathogens in patients with severe acute pancreatitis: a retrospective study.

BMC Gastroenterol 2020 Dec 1;20(1):405. Epub 2020 Dec 1.

Tianjin Nankai Hospital, Nankai Clinical School of Medicine, Tianjin Medical University, Tianjin, China.

Background: Infection is one of the important causes of death in patients with severe acute pancreatitis (SAP), but the bacterial spectrum and antibiotic resistance are constantly changing. Making good use of antibiotics and controlling multi-drug-resistant (MDR) bacterial infections are of vital importance in improving the cure rate of SAP. We conducted a retrospective study in the hope of providing references for antibiotic selection and control of drug-resistant bacteria.

Methods: Retrospective analysis was performed on the data of patients hospitalized in our hospital due to acute pancreatitis (AP) in the past 5 years. General data were classified and statistically analyzed. Subsequently, the bacterial spectrum characteristics and the data related to drug-resistant bacterial infection of 569 AP patients were analyzed. Finally, unconditional logistic regression analysis was conducted to analyze the risk factors of MDR infection.

Results: A total of 398 patients were enrolled in this study and the hospitalization data and associated results were analyzed. A total of 461 strains of pathogenic bacteria were detected, including 223 (48.4%) gram-negative bacterial strains, 190 (41.2%) gram-positive bacterial strains and 48 (10.4%) fungal strains. The detection rates of resistance in gram-negative and gram-positive bacterial strains were 48.0% (107/223) and 25.3% (48/190), respectively. There were significant differences between the MDR group and the non-MDR group for the factors of precautionary antibiotic use, kinds of antibiotics used, receipt of carbapenem, tracheal intubation, hemofiltration and number of hospitalization days in the intensive care unit. Unconditional logistic regression revealed 2 risk factors for MDR bacterial infection.

Conclusions: Our results illustrate that gram-negative bacteria were the most common pathogens in SAP infection, and the proportion of gram-positive bacteria increased notably. The rate of antibiotic resistance was higher than previously reported. Unconditional logistic regression analysis showed that using more types of antibiotics and the number of hospitalization days in the ICU were the risk factors associated with MDR bacterial infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12876-020-01563-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709241PMC
December 2020

Compositional and drug-resistance profiling of pathogens in patients with severe acute pancreatitis: a retrospective study.

BMC Gastroenterol 2020 Dec 1;20(1):405. Epub 2020 Dec 1.

Tianjin Nankai Hospital, Nankai Clinical School of Medicine, Tianjin Medical University, Tianjin, China.

Background: Infection is one of the important causes of death in patients with severe acute pancreatitis (SAP), but the bacterial spectrum and antibiotic resistance are constantly changing. Making good use of antibiotics and controlling multi-drug-resistant (MDR) bacterial infections are of vital importance in improving the cure rate of SAP. We conducted a retrospective study in the hope of providing references for antibiotic selection and control of drug-resistant bacteria.

Methods: Retrospective analysis was performed on the data of patients hospitalized in our hospital due to acute pancreatitis (AP) in the past 5 years. General data were classified and statistically analyzed. Subsequently, the bacterial spectrum characteristics and the data related to drug-resistant bacterial infection of 569 AP patients were analyzed. Finally, unconditional logistic regression analysis was conducted to analyze the risk factors of MDR infection.

Results: A total of 398 patients were enrolled in this study and the hospitalization data and associated results were analyzed. A total of 461 strains of pathogenic bacteria were detected, including 223 (48.4%) gram-negative bacterial strains, 190 (41.2%) gram-positive bacterial strains and 48 (10.4%) fungal strains. The detection rates of resistance in gram-negative and gram-positive bacterial strains were 48.0% (107/223) and 25.3% (48/190), respectively. There were significant differences between the MDR group and the non-MDR group for the factors of precautionary antibiotic use, kinds of antibiotics used, receipt of carbapenem, tracheal intubation, hemofiltration and number of hospitalization days in the intensive care unit. Unconditional logistic regression revealed 2 risk factors for MDR bacterial infection.

Conclusions: Our results illustrate that gram-negative bacteria were the most common pathogens in SAP infection, and the proportion of gram-positive bacteria increased notably. The rate of antibiotic resistance was higher than previously reported. Unconditional logistic regression analysis showed that using more types of antibiotics and the number of hospitalization days in the ICU were the risk factors associated with MDR bacterial infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12876-020-01563-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7709241PMC
December 2020

Trimodal Therapy vs. Radical Cystectomy for Muscle-Invasive Bladder Cancer: A Meta-Analysis.

Front Oncol 2020 14;10:564779. Epub 2020 Oct 14.

The Second Clinical College of Lanzhou University, Lanzhou, China.

To compare the difference between trimodal therapy (TMT) and radical cystectomy (RC) in treating muscle-invasive bladder cancer, we performed a meta-analysis for data from the following database. We searched PubMed, Chinese biomedicine literature database, the Cochrane Library, China National Knowledge Internet databases, Wanfang databases, and Google Scholar up to December 2019. The main outcome measures assessed were overall survival (OS), cancer-specific survival (CSS), mortality, and Charlson comorbidity score (CCS). Two authors independently evaluated the study quality and extracted data. All data were analyzed using Review Manager (version 5.3). After database retrieval, article selection, data extraction, and quality assessment, nine articles comprising 5,721 cases from the TMT group and 48,262 cases from the RC group were included in this study. The data showed that there was no statistical difference between TMT and RC at <10 years OS [pooled hazard ratio (HR) = 1.26, 95% confidence interval (CI): 0.92-1.73, = 1.46, = 0.14], while OS of the RC group was higher than that of the TMT group at more than 10 years (pooled HR = 1.34, 95% CI: 1.18-1.54, = 4.33, < 0.0001). As for CSS, compared with the TMT group, the patients in the RC group had longer CSS (pooled HR = 1.50, 95% CI: 1.29-1.76, = 5.15, < 0.00001). Compared with RC, TMT is linked to an obvious increase in all-cause mortality and bladder-specific cancer mortality (pooled HR = 1.30, 95% CI: 1.16-1.46, = 4.55, < 0.00001; pooled HR = 1.32, 95% CI: 1.15-1.51, = 3.92, < 0.0001). The bladder cancer patients belonging to CCS "0" score preferred RC [pooled relative risk (OR) = 0.94, 95% CI: 0.89-0.98, = 2.79, = 0.005], while CCS "2" score's patients were prone to TMT (pooled OR = 1.40, 95% CI: 1.29-1.53, = 7.73, < 0.00001). Overall, this meta-analysis suggests that the efficacy of TMT is non-inferior to that of RC at <10-year OS, and RC is superior to TMT at more than 10-year OS. Therefore, TMT may be a reasonable treatment option in well-selected patients who are unsuitable for surgery or are not willing to experience surgery. In the future, more high-quality, large-sample randomized controlled trials (RCTs) are needed to verify the results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.564779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591759PMC
October 2020

Design of a robot-assisted system for transforaminal percutaneous endoscopic lumbar surgeries: study protocol.

J Orthop Surg Res 2020 Oct 19;15(1):479. Epub 2020 Oct 19.

Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.

Background: Transforaminal percutaneous endoscopic lumbar surgeries (PELS) for lumbar disc herniation and spinal stenosis are growing in popularity. However, there are some problems in the establishment of the working channel and foraminoplasty such as nerve and blood vessel injuries, more radiation exposure, and steeper learning curve. Rapid technological advancements have allowed robotic technology to assist surgeons in improving the accuracy and safety of surgeries. Therefore, the purpose of this study is to develop a robot-assisted system for transforaminal PELS, which can provide navigation and foraminoplasty.

Methods: The robot-assisted system consists of three systems: preoperative planning system, navigation system, and foraminoplasty system. In the preoperative planning system, 3D visualization of the surgical segment and surrounding tissues are realized using the multimodal image fusion technique of computed tomography and magnetic resonance imaging, and the working channel planning is carried out to reduce the risk for injury to vital blood vessels and nerves. In the navigation system, the robot can obtain visual perception ability from a visual receptor and automatically adjust the robotic platform and robot arm to the appropriate positions according to the patient's position and preoperative plan. In addition, the robot can automatically register the surgical levels through intraoperative fluoroscopy. After that, the robot will provide navigation using the 6 degree-of-freedom (DOF) robot arm according to the preoperative planning system and guide the surgeon to complete the establishment of the working channel. In the foraminoplasty system, according to the foraminoplasty planning in the preoperative planning system, the robot performs foraminoplasty automatically using the high speed burr at the end of the robot arm. The system can provide real-time feedback on the working status of the bur through multi-mode sensors such as multidimensional force, position, and acceleration. Finally, a prototype of the system is constructed and performance tests are conducted.

Discussion: Our study will develop a robot-assisted system to perform transforaminal PELS, and this robot-assisted system can also be used for other percutaneous endoscopic spinal surgeries such as interlaminar PELS and percutaneous endoscopic cervical and thoracic surgeries through further research. The development of this robot-assisted system can be of great significance. First, the robot can improve the accuracy and efficiency of endoscopic spinal surgeries. In addition, it can avoid multiple intraoperative fluoroscopies, minimize exposure to both patients and the surgical staff, shorten the operative time, and improve the learning curve of beginners, which is beneficial to the popularization of percutaneous endoscopic spinal surgeries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13018-020-02003-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7569762PMC
October 2020

Somatic Mutation Profiling of Intrahepatic Cholangiocarcinoma: Comparison between Primary and Metastasis Tumor Tissues.

J Oncol 2020 17;2020:5675020. Epub 2020 Sep 17.

Organ Transplant Center, The Affiliated Hospital of Qingdao University, Qingdao, China.

Introduction: Intrahepatic cholangiocarcinoma (ICC) exhibited increasing incidence and mortality around the world, with a 35% five-year survival rate. In this study, the genetic alteration of primary ICC and metastasis ICC was exhibited to discover novel personalized treatment strategies to improve the clinical prognosis.

Methods: Based on 153 primary and 49 metastasis formalin-fixed paraffin-embedded ICC samples, comprehensive genomic profiling was carried out.

Results: In primary tumor samples (PSs) and metastasis tumor samples (MSs), the top alteration genes were TP53 (41.8% vs 36.7%), KRAS (30.7% vs 36.7%), and ARID1A (22.2% vs 14.2%). In the top 20 most frequent alteration genes, BRAF showed lower mutation frequency in MSs as compared to PSs (0 vs 11.1%, =0.015), while LRP1B exhibited opposed trend (22.4% vs 10.4%, =0.032). In PSs, patients with MSI-H showed all PDL1 negative, and patients with PDL1 positive exhibited MSS both in PSs and MSs. It was found that the Notch pathway had more alteration genes in MSI-H patients (=0.027). Furthermore, the patients with mutated immune genes in PSs were more than that in MSs (28.8% vs 8.2%, =0.003, odd ratio = 0.2). Interestingly, the platinum drug resistance pathway was only enriched by mutated genes of MSs.

Conclusions: In this study, the identification of two meaningful mutated genes, BRAF and LRP1B, highly mutated immune gene harbored by primary ICC patients. Both in PSs and MSs, no patients with MSI-H showed PDL1 positive. The Notch pathway had more alteration genes in patients with MSI-H. And the enrichment of the platinum drug resistance pathway in MSs might offer reference for the novel therapeutic strategy of ICC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/5675020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7519439PMC
September 2020

Spatial mapping of the collagen distribution in human and mouse tissues by force volume atomic force microscopy.

Sci Rep 2020 09 24;10(1):15664. Epub 2020 Sep 24.

Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, 417E 68th St, New York, NY, 10065, USA.

Changes in the elastic properties of living tissues during normal development and in pathological processes are often due to modifications of the collagen component of the extracellular matrix at various length scales. Force volume AFM can precisely capture the mechanical properties of biological samples with force sensitivity and spatial resolution. The integration of AFM data with data of the molecular composition contributes to understanding the interplay between tissue biochemistry, organization and function. The detection of micrometer-size, heterogeneous domains at different elastic moduli in tissue sections by AFM has remained elusive so far, due to the lack of correlations with histological, optical and biochemical assessments. In this work, force volume AFM is used to identify collagen-enriched domains, naturally present in human and mouse tissues, by their elastic modulus. Collagen identification is obtained in a robust way and affordable timescales, through an optimal design of the sample preparation method and AFM parameters for faster scan with micrometer resolution. The choice of a separate reference sample stained for collagen allows correlating elastic modulus with collagen amount and position with high statistical significance. The proposed preparation method ensures safe handling of the tissue sections guarantees the preservation of their micromechanical characteristics over time and makes it much easier to perform correlation experiments with different biomarkers independently.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-72564-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518416PMC
September 2020

Intermediate-phase-assisted low-temperature formation of γ-CsPbI films for high-efficiency deep-red light-emitting devices.

Nat Commun 2020 Sep 21;11(1):4736. Epub 2020 Sep 21.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, China.

Black phase CsPbI is attractive for optoelectronic devices, while usually it has a high formation energy and requires an annealing temperature of above 300 °C. The formation energy can be significantly reduced by adding HI in the precursor. However, the resulting films are not suitable for light-emitting applications due to the high trap densities and low photoluminescence quantum efficiencies, and the low temperature formation mechanism is not well understood yet. Here, we demonstrate a general approach for deposition of γ-CsPbI films at 100 °C with high photoluminescence quantum efficiencies by adding organic ammonium cations, and the resulting light-emitting diode exhibits an external quantum efficiency of 10.4% with suppressed efficiency roll-off. We reveal that the low-temperature crystallization process is due to the formation of low-dimensional intermediate states, and followed by interionic exchange. This work provides perspectives to tune phase transition pathway at low temperature for CsPbI device applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-020-18380-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505955PMC
September 2020

gC1qR/HABP1/p32 Is a Potential New Therapeutic Target Against Mesothelioma.

Front Oncol 2020 12;10:1413. Epub 2020 Aug 12.

Department of Surgery, Thoracic Service, Memorial Sloan Kettering Cancer Center, New York, NY, United States.

Mesothelioma is an aggressive cancer of the serous membranes with poor prognosis despite combination therapy consisting of surgery, radiotherapy, and platinum-based chemotherapy. Targeted therapies, including immunotherapies, have reported limited success, suggesting the need for additional therapeutic targets. This study investigates a potential new therapeutic target, gC1qR/HABP1/p32 (gC1qR), which is overexpressed in all morphologic subtypes of mesothelioma. gC1qR is a complement receptor that is associated with several cellular functions, including cell proliferation and angiogenesis. and experiments were conducted to test the hypothesis that targeting gC1qR with a specific gC1qR monoclonal antibody 60.11 reduces mesothelioma tumor growth, using the biphasic mesothelioma cell line MSTO-211H (MSTO). studies demonstrate cell surface and extracellular gC1qR expression by MSTO cells, and a modest 25.3 ± 1.8% ( = 4) reduction in cell proliferation by the gC1qR blocking 60.11 antibody. This inhibition was specific for targeting the C1q binding domain of gC1qR at aa 76-93, as a separate monoclonal antibody 74.5.2, directed against amino acids 204-218, had no discernable effect. studies, using a murine orthotopic xenotransplant model, demonstrated an even greater reduction in MSTO tumor growth (50% inhibition) in mice treated with the 60.11 antibody compared to controls. Immunohistochemical studies of resected tumors revealed increased cellular apoptosis by caspase 3 and TUNEL staining, in 60.11 treated tumors compared to controls, as well as impaired angiogenesis by decreased CD31 staining. Taken together, these data identify gC1qR as a potential new therapeutic target against mesothelioma with both antiproliferative and antiangiogenic properties.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2020.01413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7435067PMC
August 2020

Novel strategy to monitor fluid absorption and blood loss during urological endoscopic surgery.

Transl Androl Urol 2020 Jun;9(3):1192-1200

Department of Urology, The Second Hospital of Lanzhou University, Lanzhou, China.

Background: There is paucity of an optimal method to detect fluid absorption and hemorrhage during urological endoscopic surgery. We designed an endoscopic surgical monitoring system (ESMS) and estimated its performance to establish a practical instrument that can monitor the blood loss and fluid absorption accurately and non-invasively during urological endoscopic surgery.

Methods: Our system employed the strain gauge transducers to detect the inflows and outflows of the irrigating solutions and the photoelectric sensor to determine the hemoglobin concentration of the collected irrigating fluid. The amount of blood lost and the volume of fluid absorbed during endoscopic surgery could be calculated by computer program. The accuracy and validity of this system were validated in simulated experiment and clinical study of 200 patients who underwent transurethral resection of the prostate (TURP).

Results: The relative errors for fluid absorption detection were between 0.07% and 1.00% and the coefficient of variation in serial analysis ranged from 0.78% to 3.86%. Furthermore, the relative errors for blood loss detection were between 0.06% and 1.33% and the coefficient of variation in serial analysis ranged from 0.86% to 3.94%. In clinical study for TURP, the mean fluid absorption was 644 mL and blood loss was 238 mL.

Conclusions: We provide the accuracy and validity of ESMS. It provides an early and real time detection and warning of irrigation fluid absorption and blood loss to make endoscopic surgical procedure safer for the patient.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/tau-19-780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7354347PMC
June 2020

The effect of ursodeoxycholic acid on the relative expression of the lipid metabolism genes in mouse cholesterol gallstone models.

Lipids Health Dis 2020 Jul 2;19(1):158. Epub 2020 Jul 2.

Department of Surgery, Tianjin Nankai Hospital, Nankai Clinical School of Medicine, Tianjin Medical University, 122 Sanwei Road Nankai District, Tianjin, 300100, China.

Background: Many studies indicate that gallstone formation has genetic components. The abnormal expression of lipid-related genes could be the basis for particular forms of cholesterol gallstone disease. The aim of this study was to obtain insight into lipid metabolism disorder during cholesterol gallstone formation and to evaluate the effect of ursodeoxycholic acid (UDCA) on the improvement of bile lithogenicity and its potential influence on the transcription of lipid-related genes.

Methods: Gallstone-susceptible mouse models were induced by feeding with a lithogenic diet (LD) for 8 weeks. Bile and liver tissues were obtained from these mouse models after 0, 4 and 8 weeks. Bile lipids were measured enzymatically, and the cholesterol saturation index (CSI) was calculated to evaluate the bile lithogenicity by using Carey's critical tables. Real-time polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of farnesoid X receptor (FXR), liver X receptor (LXR), adenosine triphosphate-binding cassette subfamily G member 5/8 (ABCG5/8), cholesterol 7-α hydroxylase (CYP7A1), oxysterol 7-α hydroxylase (CYP7B1), sterol 27-α hydroxylase (CYP27A1), peroxisome proliferator-activated receptor alpha (PPAR-α) and adenosine triphosphate-binding cassette subfamily B member 11 (ABCB11).

Results: The rate of gallstone formation was 100% in the 4-week group but only 30% in the UDCA-treated group. The UDCA-treated group had a significantly lower CSI compared with other groups. Of special note, the data on the effects of UDCA showed higher expression levels of ABCG8, ABCB11 and CYP27A1, as well as lower expression levels of LXR and PPAR-α, compared to the model control group.

Conclusions: UDCA exhibits tremendously potent activity in restraining lipid accumulation, thus reversing the lithogenic effect and protecting hepatocytes from serious pathological damage. The abnormal expression of ABCG8, CYP7A1, CYP27A1, LXR and PPAR-α might lead to high lithogenicity of bile. These results are helpful in exploring new lipid metabolism pathways and potential targets for the treatment of cholesterol stones and for providing some basis for the study of the pathogenesis and genetic characteristics of cholelithiasis. Research on the mechanism of UDCA in improving lipid metabolism and bile lithogenicity may be helpful for clinical treatment and for reducing the incidence of gallstones.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12944-020-01334-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333299PMC
July 2020

scAAV2-Mediated C3 Transferase Gene Therapy in a Rat Model with Retinal Ischemia/Reperfusion Injuries.

Mol Ther Methods Clin Dev 2020 Jun 25;17:894-903. Epub 2020 Apr 25.

Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, School of Optometry, Shenzhen University, Shenzhen 518000, China.

Glaucoma is characterized by retinal ganglion cell (RGC) death and axonal loss. Therefore, neuroprotection is important in treating glaucoma. In this study, we explored whether exoenzyme C3 transferase (C3)-based gene therapy could protect retinas in an ischemia/reperfusion (I/R) injury rat model. Self-complementary adeno-associated virus 2 (scAAV2) vectors encoding either C3 protein (scAAV2-C3) or enhanced green fluorescence protein (scAAV2-EGFP) were intravitreally delivered into both eyes of rats, and I/R models (acute ocular hypertension) were made in one eye of each rat at day 7 after the injection. The rats were divided into six groups: scAAV2-C3, scAAV2-C3 with I/R, scAAV2-EGFP, scAAV2-EGFP with I/R, blank control, and blank control with I/R. TUNEL (terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick end labeling), immunohistochemistry of cleaved caspase-3, NeuN and Brn-3a, and H&E staining were used to detect apoptotic cells and other changes in the retina. The results showed that scAAV2-C3 significantly reduced the number of apoptotic RGCs and decreased cell loss in the ganglion cell layer after I/R injury, and the I/R-injured retinas treated with scAAV2-C3 were the thickest in all I/R groups. These results suggest that scAAV2-mediated C3 gene therapy is able to protect the rat retina from I/R injury and has potential in the treatment of glaucoma in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.omtm.2020.04.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7200613PMC
June 2020

SAP97 polymorphisms associated with early onset Parkinson's disease.

Neurosci Lett 2020 05 26;728:134931. Epub 2020 Mar 26.

Maternal and Children's Health Research Institute, Shunde Maternal and Children's Hospital, Guangdong Medical University, Foshan, 528300, China; Clinical Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, China. Electronic address:

Objective: To investigate the relationship between SAP97 genetic polymorphisms and sporadic Parkinson's disease (PD) in Han Chinese population with the expectation of offering genetic data for the early prevention and treatment of the disease.

Methods: In this study, we genotyped single-nucleotide polymorphisms (SNPs) (rs3915512 and rs9843659) in theSAP97 gene in 317 patients with PD and 317 healthy-matched controls in a Han Chinese population through the improved multiplex ligation detection reaction (imLDR) technique. Then, we analyzed the association of each SNP, alone or in combination, with risk or age of onset of PD.

Results: The SAP97 rs3915512 and rs9843659 polymorphisms were not associated with the risk of PD. However, the minor allele of the rs3915512 and rs9843659 were significantly more common in PD patients with an early age of onset. Additionally, significant differences in the distribution of the onset age of the PD among different genotypes of the rs9843659 polymorphism. The CA haplotype was significantly related to early onset PD.

Conclusions: Our data are the first to suggest that the SAP97 SNPs rs3915512 and rs9843659 and the CA haplotype may be significantly associated with early onset PD in China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neulet.2020.134931DOI Listing
May 2020

Effect of different high altitudes on vascular endothelial function in healthy people.

Medicine (Baltimore) 2020 Mar;99(11):e19292

The Affiliated Hospital of Qing Hai University, Xi Ning, Qing Hai, China.

Background: The aim of the study was to provide a theoretical basis for the early diagnosis and prediction of acute altitude sickness, to provide a better entry mode for healthy people from plain areas to plateau areas, and to preliminarily clarify the possible mechanism of this approach.

Methods: We measured endothelin-1 (ET-1), asymmetric dimethylarginine (ADMA), vascular endothelial growth factor (VEGF), nitric oxide (NO), and hypoxia-inducible factor 1 (HIF-1) levels in each sample and determined flow-mediated dilation (FMD) values using a portable OMRON color Doppler with a 7.0- to 12.0-MHz linear array probe. We used the Lewis Lake score to diagnose acute mountain sickness (AMS) and to stratify the disease severity.

Results: We found no cases of AMS at any of the studied elevation gradients. We found significant differences in FMD values between individuals when at 400 m above sea level and when at 2200, 3200, and 4200 m above sea level (P < .05) but found no significant differences among those at 2200, 3200, and 4200 m. Our variance analysis showed that serum ET-1, VEGF, ADMA, NO, and HIF-1 levels in individuals at ≥3000 m and those at subplateau and plain areas (<3000 m) significantly differed (P < .05). The level of these factors also significantly differed between individuals at elevation gradients of plateau areas (3260 m vs 4270 m) (P < .05). We found no significant differences in serum ET-1, VEGF, and ADMA levels between individuals at the plateau (2260 m) and plain (400 m) areas (P > .05). NO and HIF-1 levels were significantly different in serum samples from individuals between the plateau (2260 m) and plain (400 m) areas (P < .05). However, with increasing altitude, the NO level gradually increased, whereas ET-1, ADMA, VEGF, and HIF-1 levels showed a decreasing trend. With the increase of altitude, there is no correlation between the trend of FMD and hematologic-related factors such as VEGF, NO, and HIF-1.

Conclusion: A healthy young male population ascending to a high-altitude area experiences a low incidence of AMS. Entering an acute plateau exposure environment from different altitude gradients may weaken the effect of acute highland exposure on vascular endothelial dysfunction in healthy individuals. Changes in serum ET-1, VEGF, ADMA, NO, and HIF-1 levels in healthy young men may be related to the body's self-regulation and protect healthy individuals from AMS. A short stay in a subplateau region may initiate an oxygen-free preconditioning process in healthy individuals, thereby protecting them from AMS. Noninvasive brachial artery endothelial function test instead of the detection of invasive hematologic-related factors for early diagnosis and prediction of the occurrence and severity of acute high-altitude disease is still lack of sufficient theoretical basis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000019292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220113PMC
March 2020

C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey.

Mol Ther Methods Clin Dev 2020 Jun 29;17:143-155. Epub 2019 Nov 29.

Xiamen Eye Center, Xiamen University, Xiamen, China.

Glaucoma is a lifelong disease with elevated intraocular pressure (IOP) as the main risk factor, and reduction of IOP remains the major treatment for this disease. However, current IOP-lowering therapies are far from being satisfactory. We have demonstrated that the lentivirus-mediated exoenzyme C3 transferase (C3) expression in rat and monkey eyes induced relatively long-term IOP reduction. We now show that intracameral injection of self-complementary AAV2 containing a C3 gene into mouse and monkey eyes resulted in morphological changes in trabecular meshwork and IOP reduction. The vector-transduced corneal endothelium and the C3 transgene expression, not vector itself, induced corneal edema as a result of actin-associated endothelial barrier disruption. There was a positive (quadratic) correlation between measured IOP and grade of corneal edema. This is the first report of using an AAV to transduce the trabecular meshwork of monkeys with a gene capable of altering cellular structure and physiology, indicating a potential gene therapy for glaucoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.omtm.2019.11.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938898PMC
June 2020

Is "normal tension glaucoma" glaucoma?

Med Hypotheses 2019 Dec 24;133:109405. Epub 2019 Sep 24.

Xiamen Eye Center, Xiamen University, China. Electronic address:

Primary open angle glaucoma (POAG) represents a distinct disease entity with elevated intraocular pressure (IOP) as the main risk factor, even though the reasons for why the IOP is elevated remains to be elucidated. It is considered that normal tension glaucoma (NTG) is a subtype of POAG, comprising a special form of glaucomatous neurodegeneration or glaucomatous optic neuropathy (GON) almost exactly the same as that seen in POAG, but the IOP, as named, remains in the statistically normal range. Actually the disease entity of NTG has been a profound confusion and it is difficult to be accurately conceptualized. One of the reasons is that the IOP is closely linked to the occurrence of GON in POAG but not in NTG, and for the latter, it seems that GON is secondary to a number of local or systemic disorders. In recent years, increasing evidences suggest that NTG or IOP independent GON is a non-glaucomatous disease with different disease entities from POAG and with more diverse and complex etiologies. Here we hypothesized that NTG, at least for those with recognizable primary diseases, is not a glaucomatous disease; instead, it represents a group of disorders with GON as a characteristic clinical feature or phenotype.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.mehy.2019.109405DOI Listing
December 2019

Cognitive mechanism of intimate interpersonal relationships and loneliness in internet-addicts: An ERP study.

Addict Behav Rep 2019 Dec 24;10:100209. Epub 2019 Jul 24.

Institute of Developmental Psychology, Beijing Normal University, Beijing, China.

Interpersonal relationship and loneliness are important factors affecting internet addictive behavior of individuals. In the present study, we investigated intimate interpersonal relationships and loneliness in internet-addicts. We recorded event-related potentials (ERPs) of 32 internet addicts and 32 non internet-addicts. Participants viewed intimate-/conflict-relationship, happy/lonely, and neutral images. Results concerning attention probes showed that the accuracy rate of attention probes of internet-addicts was significantly lower than that of non internet-addicts; whereas, there was no significant difference in the reaction time of attention probes. Moreover, the differences in the mean amplitude and latency of P1, N1, N2P3, and LPP between internet-addicts and non internet-addicts were insignificant. Then, we found that the P1 amplitude of images was significantly higher than that of images among non internet-addicts; whereas internet-addicts indicated an insignificant difference between the two types of images. The P1 amplitude of images was significantly higher than that of images among internet-addicts, but non internet-addicts were insignificant. The questionnaire data also obtained similar conclusions based on the EEG data. Finally, internet-addicts reported significantly higher loneliness scores than those of non internet-addicts. These results suggested that the social cognitive function of internet-addicts was probably impaired, especially in the cognition of interpersonal conflict. Furthermore, internet-addicts are likely to keep poor interpersonal relationships, which may induce more loneliness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.abrep.2019.100209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6706635PMC
December 2019

Numerical Analysis and Experimental Studies on the Residual Stress of W/2024Al Composites.

Materials (Basel) 2019 Aug 27;12(17). Epub 2019 Aug 27.

Institute of advanced electronic materials, General Research Institute for Nonferrous Metals, Beijing 100088, China.

W/2024Al composites can be used for radiation shielding with desirable mechanical properties such as high strength, excellent corrosion resistance, and low density. The quench-induced residual stresses in W/2024Al composites were studied by experimental measurements and numerical analysis using ABAQUS software. Due to the accurate calculation of heat transfer coefficients and the established constitutive equation for description of the variation of yield stress at elevated temperature with different strain rates, the prediction of residual stresses in as-quenched composite blocks achieved by finite element method (FEM) is reliable. Moreover, X-ray diffraction and crack-compliance method were carried out to measure the stresses that developed at the surface and interior of the composites to validate the simulation results. Quenching residual stresses of composite blocks were investigated by taking the influence of quenching medium temperature into consideration. In addition, a comparative study on residual stress magnitudes of as-quenched 2024Al and W/2024Al composites was conducted, and the results show that stress magnitudes of W/2024Al composites are lower than that of 2024Al due to lower thermal gradients during the quenching process.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma12172746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747812PMC
August 2019

Lentiviral Vector-Mediated Expression of Exoenzyme C3 Transferase Lowers Intraocular Pressure in Monkeys.

Mol Ther 2019 07 9;27(7):1327-1338. Epub 2019 May 9.

Xiamen Eye Center, Xiamen University, Xiamen, China; Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, School of Optometry, Shenzhen University, Shenzhen, China. Electronic address:

Primary open-angle glaucoma (POAG) is considered a lifelong disease characterized by optic nerve deterioration and visual field damage. Although the disease progression can usually be controlled by lowering the intraocular pressure (IOP), therapeutic effects of current approaches do not last long. Gene therapy could be a promising method for persistent treatment of the disease. Our previous study demonstrated that gene transfer of exoenzyme C3 transferase (C3) to the trabecular meshwork (TM) to inhibit Rho GTPase (Rho), the upstream signal molecule of Rho-associated kinase (ROCK), resulted in lowered IOP in normal rodent eyes. In the present study, we show that the lentiviral vector (LV)-mediated C3 expression inactivates RhoA in human TM cells by ADP ribosylation, resulting in disruption of the actin cytoskeleton and altered cell morphology. In addition, intracameral delivery of the C3 vector to monkey eyes leads to persistently lowered IOP without obvious signs of inflammation. This is the first report of using a vector to transduce the TM of an alive non-human primate with a gene that alters cellular machinery and physiology. Our results in non-human primates support that LV-mediated C3 expression in the TM may have therapeutic potential for glaucoma, the leading cause of irreversible blindness in humans.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ymthe.2019.04.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6612778PMC
July 2019

Tin-Based Multiple Quantum Well Perovskites for Light-Emitting Diodes with Improved Stability.

J Phys Chem Lett 2019 Feb 16;10(3):453-459. Epub 2019 Jan 16.

Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) , Nanjing Tech University (NanjingTech) , 30 South Puzhu Road , Nanjing 211816 , China.

Tin-based halide perovskites have attracted considerable attention for nontoxic perovskite light-emitting diodes (PeLEDs), but the easy oxidation of Sn and nonuniform film morphology cause poor device stability and reproducibility. Herein, we report a facile approach to achieve efficient and stable lead-free PeLEDs by using tin-based perovskite multiple quantum wells (MQWs) for the first time. On the basis of various spectroscopic investigations, we find that the MQW structure not only facilitates the formation of uniform and highly emissive perovskite films but also suppresses the oxidation of Sn cations. The tin-based MQW PeLED exhibits a peak external quantum efficiency of 3% and a high radiance of 40 W sr m with good reproducibility. Significantly, these devices show excellent operational stability with over a 2 h lifetime under a constant current density of 10 mA cm, which is comparable to that of lead-based PeLEDs. These results suggest that perovskite MQWs can provide a promising platform for achieving high-performance lead-free PeLEDs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.8b03700DOI Listing
February 2019

Effects of Lentivirus-Mediated C3 Expression on Trabecular Meshwork Cells and Intraocular Pressure.

Invest Ophthalmol Vis Sci 2018 10;59(12):4937-4944

Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, School of Optometry, Shenzhen University, Shenzhen, China.

Purpose: We evaluated the effects of lentivirus-mediated exoenzyme C3 transferase (C3) expression on cultured primary human trabecular meshwork (HTM) cells in vitro, and on rat intraocular pressure (IOP).

Methods: HTM cells were cultured and treated with lentivirus vectors expressing either green fluorescent protein (GFP) only (LV-GFP) or GFP and C3 together (LV-C3-GFP). Changes in cell morphology and actin stress fibers were assessed. The vectors were also injected into the anterior chamber of rats, and GFP expression was visualized by a Micron III Retinal Imaging Microscope in vivo and a fluorescence microscope ex vivo. Changes in rat IOP were monitored by using a rebound tonometer and the eyes were evaluated by slit lamp.

Results: LV-mediated C3 expression induced morphologic changes in HTM cells. The cells became retracted and rounded. GFP expression in the anterior chamber angle of rats was observed in vivo from 8 days to 48 days after injection of LV-C3-GFP or LV-GFP. IOP was significantly decreased in the LV-C3-GFP group starting 3 days post injection, and lasting for at least 40 days, when compared to either the contralateral control eyes (the LV-GFP group) or the ipsilateral baseline before injection (P < 0.05). No obvious inflammatory signs were observed in either the LV-C3-GFP or LV-GFP groups.

Conclusions: LV-mediated C3 expression induced changes in morphology of cultured HTM cells. Intracameral injection of LV-C3-GFP lowered rat IOP for at least 40 days. No significant inflammatory reactions were observed in either the LV-C3-GFP or LV-GFP groups. This study supports the possible use of C3 gene therapy for the treatment of glaucoma.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1167/iovs.18-24978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6187949PMC
October 2018

A case of type II Mirizzi syndrome treated by simple endoscopic means.

J Surg Case Rep 2018 Oct 8;2018(10):rjy257. Epub 2018 Oct 8.

Department of Surgery, Yan Chai Hospital, Hong Kong, China.

Mirizzi syndrome is an uncommon complication of chronic cholelithiasis. Advancement in radiological modalities and minimally invasive surgery has led to improved pre-operative diagnoses and more laparoscopic cholecystectomies. But for unsuitable surgical candidates, endoscopy can be the definitive treatment. In this case, we present a 67-year-old man with type II Mirizzi syndrome treated by simple endoscopic means.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jscr/rjy257DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6174627PMC
October 2018

[Progression on finite element modeling method in scoliosis].

Zhongguo Gu Shang 2018 Apr;31(4):391-394

Department of Orthopaedics, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing 100020, China;

Scoliosis is a complex spinal three-dimensional malformation with complicated pathogenesis, often associated with complications as thoracic deformity and shoulder imbalance. Because the acquisition of specimen or animal models are difficult, the biomechanical study of scoliosis is limited. In recent years, along with the development of the computer technology, software and image, the technology of establishing a finite element model of human spine is maturing and it has been providing strong support for the research of pathogenesis of scoliosis, the design and application of brace, and the selection of surgical methods. The finite element model method is gradually becoming an important tool in the biomechanical study of scoliosis. Establishing a high quality finite element model is the basis of analysis and future study. However, the finite element modeling process can be complex and modeling methods are greatly varied. Choosing the appropriate modeling method according to research objectives has become researchers' primary task. In this paper, the author reviews the national and international literature in recent years and concludes the finite element modeling methods in scoliosis, including data acquisition, establishment of the geometric model, the material properties, parameters setting, the validity of the finite element model validation and so on.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3969/j.issn.1003-0034.2018.04.018DOI Listing
April 2018

An In Vivo Murine Sciatic Nerve Model of Perineural Invasion.

J Vis Exp 2018 04 23(134). Epub 2018 Apr 23.

Department of Surgery, Memorial Sloan Kettering Cancer Center.

Cancer cells invade nerves through a process termed perineural invasion (PNI), in which cancer cells proliferate and migrate in the nerve microenvironment. This type of invasion is exhibited by a variety of cancer types, and very frequently is found in pancreatic cancer. The microscopic size of nerve fibers within mouse pancreas renders the study of PNI difficult in orthotopic murine models. Here, we describe a heterotopic in vivo model of PNI, where we inject syngeneic pancreatic cancer cell line Panc02-H7 into the murine sciatic nerve. In this model, sciatic nerves of anesthetized mice are exposed and injected with cancer cells. The cancer cells invade in the nerves proximally toward the spinal cord from the point of injection. The invaded sciatic nerves are then extracted and processed with OCT for frozen sectioning. H&E and immunofluorescence staining of these sections allow quantification of both the degree of invasion and changes in protein expression. This model can be applied to a variety of studies on PNI given its versatility. Using mice with different genetic modifications and/or different types of cancer cells allows for investigation of the cellular and molecular mechanisms of PNI and for different cancer types. Furthermore, the effects of therapeutic agents on nerve invasion can be studied by applying treatment to these mice.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3791/56857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965264PMC
April 2018

Systematic evaluation of a targeted gene capture sequencing panel for molecular diagnosis of retinitis pigmentosa.

PLoS One 2018 11;13(4):e0185237. Epub 2018 Apr 11.

BGI-Shenzhen, Shenzhen, China.

Background: Inherited eye diseases are major causes of vision loss in both children and adults. Inherited eye diseases are characterized by clinical variability and pronounced genetic heterogeneity. Genetic testing may provide an accurate diagnosis for ophthalmic genetic disorders and allow gene therapy for specific diseases.

Methods: A targeted gene capture panel was designed to capture exons of 283 inherited eye disease genes including 58 known causative retinitis pigmentosa (RP) genes. 180 samples were tested with this panel, 68 were previously tested by Sanger sequencing. Systematic evaluation of our method and comprehensive molecular diagnosis were carried on 99 RP patients.

Results: 96.85% targeted regions were covered by at least 20 folds, the accuracy of variants detection was 99.994%. In 4 of the 68 samples previously tested by Sanger sequencing, mutations of other diseases not consisting with the clinical diagnosis were detected by next-generation sequencing (NGS) not Sanger. Among the 99 RP patients, 64 (64.6%) were detected with pathogenic mutations, while in 3 patients, it was inconsistent between molecular diagnosis and their initial clinical diagnosis. After revisiting, one patient's clinical diagnosis was reclassified. In addition, 3 patients were found carrying large deletions.

Conclusions: We have systematically evaluated our method and compared it with Sanger sequencing, and have identified a large number of novel mutations in a cohort of 99 RP patients. The results showed a sufficient accuracy of our method and suggested the importance of molecular diagnosis in clinical diagnosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185237PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894961PMC
July 2018

gene involved in pathogenesis of a Chinese family with Bardet-Biedl syndrome.

Oncotarget 2017 Oct 30;8(49):86718-86725. Epub 2017 Sep 30.

Shenzhen Key Laboratory of Ophthalmology, Shenzhen Eye Hospital, Shenzhen University, Shenzhen, China.

Purpose: This study was designed to evaluate the molecular genetics of a Chinese family with Bardet-Biedl syndrome (BBS).

Methods: All the family members underwent medical history evaluation, ophthalmologic and physical examinations. Whole exome sequencing was performed on two affected individuals and their parents. All variants were verified in all family members by PCR amplification and Sanger sequencing.

Results: Patients in this family were diagnosed as Bardet-Biedl syndrome, with an inheritance pattern of autosomal recessive. Compound heterozygous mutations of the gene (c.3616G>A and c.6037C>T) were identified by whole exome sequencing. Results from Sanger sequencing showed co-segregation of these compound heterozygous mutations in the gene with BBS disease in the family.

Conclusion: Novel compound heterozygous mutations c.3616G>A and c.6037C>T of were identified in all affected individuals but not in the unaffected family members. This is the first time to the best of our knowledge, that the gene is involved in the pathogenesis of BBS. This study will expand our understanding about the gene spectrum related to this genetically heterogeneous disorder.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.18632/oncotarget.21415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689720PMC
October 2017