Publications by authors named "Ning An"

218 Publications

ER expression associates with poor prognosis in male lung squamous carcinoma after radical resection.

BMC Cancer 2021 Sep 21;21(1):1043. Epub 2021 Sep 21.

Department of Radiation Oncology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, Shandong, China.

Background: Clinical options for lung squamous carcinoma (LUSC) are still quite limited. Carcinogenesis is an exceedingly complicated process involving multi-level dysregulations. Therefore, only looking into one layer of genomic dysregulation is far from sufficient.

Methods: We identified differentially expressed genes with consistent upstream genetic or epigenetic dysregulations in LUSC. Random walk was adopted to identify genes significantly affected by upstream abnormalities. Expression differentiation and survival analysis were conducted for these significant genes, respectively. Prognostic power of selected gene was also tested in 102 male LUSC samples through immunohistochemistry assay.

Results: Twelve genes were successfully retrieved from biological network, including ERα (ESRS1), EGFR, AR, ATXN1, MAPK3, PRKACA, PRKCA, SMAD4, TP53, TRAF2, UBQLN4 and YWHAG, which were closely related to sex hormone signaling pathway. Survival analysis in public datasets indicated ERα was significantly associated with a poor overall survival (OS) in male LUSC. The result of our immunohistochemistry assay also demonstrated this correlation using R0 resected tumors (n = 102, HR: 2.152, 95% CI: 1.089-4.255, p = 0.024). Although disease-free survival (DFS) difference was non-significant (n = 102, p = 0.12), the tendency of distinction was straight-forward. Cox analysis indicated ERα was the only independent prognostic factor for male patients' OS after R0 resection (HR = 2.152, p = 0.037).

Conclusion: ERα was significantly related to a poor prognosis in LUSC, especially for male patients after radical surgery, confirmed by our immunohistochemistry data.
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http://dx.doi.org/10.1186/s12885-021-08777-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456567PMC
September 2021

bFGF-mediated phosphorylation of δ-catenin increases its protein stability and the ability to induce the nuclear redistribution of β-catenin.

Am J Cancer Res 2021 15;11(8):3877-3892. Epub 2021 Aug 15.

College of Pharmacy, Chonnam National University Gwangju 61186, Korea.

Recently, we have shown that δ-catenin strengthened the epidermal growth factor receptor (EGFR)/Erk1/2 signaling pathway through the association between EGFR and δ-catenin. Now, we further analyzed the correlation between basic fibroblast growth factor (bFGF)/fibroblast growth factor receptor 1 (FGFR1) and δ-catenin in prostate cancer and investigated the molecular mechanism underlying the role of bFGF/FGFR1 modulation in CWR22Rv-1 (Rv-1) cells. Here, we demonstrated that bFGF phosphorylated the tyrosine residues of δ-catenin in Rv-1 cells and further proved that the bFGF mediated FGFR1/δ-catenin tyrosine phosphorylation was time dependent. Furthermore, we demonstrated that bFGF stabilized the expression of δ-catenin through weakening its association with GSK3β and enhancing its stability to induce β-catenin into the nuclear by strengthening the processing of E-cadherin. In a word, these results indicated that bFGF/FGFR1 signaling pathway could enhance the tumor progression of prostate cancer via δ-catenin.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414378PMC
August 2021

Predictors of Seizure Freedom in Patients Undergoing Surgery for Central Nervous System Infection-Related Epilepsy: A Systematic Review and Meta-Analysis.

Front Neurol 2021 18;12:668439. Epub 2021 Aug 18.

Department of Neurosurgery, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing, China.

Central nervous system infections (CNSIs), especially viral encephalitis and meningitis, are well-recognized causes of medically refractory epilepsy. Although surgery is an effective and durable intervention against these infections, the seizure control outcomes described in previous surgical series have been variable. Accordingly, it is not clear which variables are most valuable in predicting seizure control following surgery for CNSI. The aim of this meta-analysis was to identify the predictors of favorable surgical outcomes in CNSI-related epilepsy. The PubMed, EMBASE, Cochrane Library, WANGFANG, VIP, CBM, and CNKI databases were searched for studies according to the inclusion criteria. Prognostic factors, surgical outcomes, and patient characteristics were extracted. Heterogeneity was detected by the I and Q statistics. Seventeen studies were included in our meta-analysis. Eight predictors of favorable outcomes (Engel Class I/II) were determined, including abnormal MRI findings, meningitis, temporal location only, regional ictal pattern, unilateral ictal pattern, older age at epilepsy, longer silent period, and longer time from infection, as follows: OR = 3.34 (95% CI 1.44-7.74), OR = 0.31 (95% CI 0.13-0.70), OR = 0.34 (95% CI 0.16-0.74), OR = 5.65 (95% CI 1.75-18.30), and OR = 9.53 (95% CI 2.36-38.48), respectively, and MD = 2.15 (95% CI 0.20-4.11), MD = 2.40 (95% CI 0.09-4.70), and MD = 8.49 (95% CI 1.50-15.48), respectively. A subgroup analysis found the following associations: regional and unilateral ictal patterns in viral encephalitis, a younger age at infection in parasitic encephalopathy, an older age at surgery, a longer time from onset, and a longer time from infection in unexplained meningitis. A sensitivity analysis restricted to studies that included each variable yielded robust results. Little evidence of publication bias was observed. This meta-analysis suggests that abnormal MRI findings, meningitis, temporal location only, regional and unilateral ictal patterns, older age at epilepsy, longer silent period, and longer time from infection are predictive factors in patients with favorable surgical outcomes in CNSI-related epilepsy. In addition, different infective agents influenced the results in regional and unilateral ictal patterns in ictal electroencephalography, as well as the relationship between age at infection and surgery and the time from epilepsy onset and infection.
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http://dx.doi.org/10.3389/fneur.2021.668439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416488PMC
August 2021

[Overexpression of mitofusion 2 inhibits acute respiratory distress syndrome pulmonary fibrosis and its mechanism].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2021 Jul;33(7):861-865

Department of Intensive Care Unit, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology (Wuhan Fourth Hospital), Wuhan 430034, Hubei, China. Corresponding author: Xu Meixia, Email:

Objective: To study the inhibitory effect of overexpression of mitofusion 2 (Mfn2) protein on acute respiratory distress syndrome (ARDS) pulmonary fibrosis and its mechanism.

Methods: Human embryo lung fibroblasts (HELF) were cultured in vitro, and digested and passaged when the adherent rate of HELF reached 80%, and then the cells in good condition were selected for experiment. The ARDS cell model was reproduced by 5 mg/L of lipopolysaccharide (LPS, LPS group); 75 mol/L adenovirus vector carrying mitofusion 2 (Adv-Mfn2) was transfected into HELF (Adv-Mfn2+LPS group); at the same time, blank control group (complete medium culture) and Adv-vector+LPS group were set as controls. The cell proliferation was observed by sulforhodamine B (SRB) method at 0, 12, 24, 36 and 48 hours. After Hoechst 33342 staining, the morphological changes were observed under confocal microscope. Western blotting was used to detect the protein expressions of Bcl-2 and caspase-3. Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the gene expressions of Bcl-2 and caspase-3.

Results: After LPS stimulation for 12-48 hours, the cell proliferation rates in the LPS group increased gradually, which were significantly higher than those in the blank control group [12 hours: (10.75±1.51)% vs. (0.73±1.22)%, 24 hours: (20.09±1.71)% vs. (1.15±1.12)%, 36 hours: (20.58±1.55)% vs. (1.20±1.12)%, 48 hours: (21.30±1.51)% vs. (1.23±1.10)%, all P < 0.01]. There was no statistically significant difference in the cell proliferation rate between the LPS group and the Adv-vector+LPS group. After overexpression of Mfn2, the cell proliferation rates at 12, 24, 36, 48 hours in the Adv-Mfn2+LPS group were (8.93±1.14)%, (10.52±1.24)%, (10.72±1.30)%, and (10.91±1.20)%, which were significantly lower than those in the LPS group (all P < 0.05). Confocal microscopy showed that some cells in the blank control group had nuclei of different sizes, and some nuclei fragmented or shrank to form apoptotic bodies. The nuclei of the cells in the LPS and Adv-vector+LPS groups were round or oval in size, and only a few apoptotic cells appeared. When Mfn2 was overexpressed, there were more apoptotic cells in the visual field in the Adv-Mfn2+LPS group than LPS group. Western blotting and RT-qPCR results showed that Bcl-2 expressions increased significantly after LPS stimulation in the LPS group as compared with the blank control group [Bcl-2 protein (Bcl-2/GAPDH): 0.68±0.01 vs. 0.29±0.01, Bcl-2 mRNA (2): 2.23±0.34 vs. 1.00±0.00, both P < 0.01], and caspase-3 expressions decreased significantly [caspase-3 protein (caspase-3/GAPDH): 0.37±0.02 vs. 0.66±0.02, caspase-3 mRNA (2): 0.31±0.05 vs. 1.00±0.00, both P < 0.01]. Compared with LPS group, the expressions of Bcl-2 after overexpression of Mfn2 in the Adv-Mfn2+LPS group were down-regulated [Bcl-2 protein (Bcl-2/GAPDH): 0.46±0.01 vs. 0.68±0.01, Bcl-2 mRNA (2): 1.45±0.14 vs. 2.23±0.34, both P < 0.01], and the expressions of caspase-3 were up-regulated [caspase-3 protein (caspase-3/GAPDH): 0.54±0.02 vs. 0.37±0.02, caspase-3 mRNA (2): 0.88±0.10 vs. 0.31±0.05, both P < 0.01].

Conclusions: Mfn2 protein is involved in ARDS pulmonary fibrosis, which may be related to mitochondrial mediated inhibition of cell proliferation.
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http://dx.doi.org/10.3760/cma.j.cn121430-20201222-00768DOI Listing
July 2021

Atomically dispersed Fe atoms anchored on S and N-codoped carbon for efficient electrochemical denitrification.

Proc Natl Acad Sci U S A 2021 Aug;118(33)

School of Environment, Tsinghua University, Beijing 100084, China.

Nitrate, a widespread contaminant in natural water, is a threat to ecological safety and human health. Although direct nitrate removal by electrochemical methods is efficient, the development of low-cost electrocatalysts with high reactivity remains challenging. Herein, bifunctional single-atom catalysts (SACs) were prepared with Cu or Fe active centers on an N-doped or S, N-codoped carbon basal plane for N or NH production. The maximum nitrate removal capacity was 7,822 mg N ⋅ g Fe, which was the highest among previous studies. A high ammonia Faradic efficiency (78.4%) was achieved at a low potential (-0.57 versus reversible hydrogen electrode), and the nitrogen selectivity was 100% on S-modified Fe SACs. Theoretical and experimental investigations of the S-doping charge-transfer effect revealed that strong metal-support interactions were beneficial for anchoring single atoms and enhancing cyclability. S-doping altered the coordination environment of single-atom centers and created numerous defects with higher conductivity, which played a key role in improving the catalyst activity. Moreover, interactions between defects and single-atom sites improved the catalytic performance. Thus, these findings offer an avenue for high active SAC design.
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http://dx.doi.org/10.1073/pnas.2105628118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8379959PMC
August 2021

Correction to: The germline/somatic DNA damage repair gene mutations modulate the therapeutic response in Chinese patients with advanced pancreatic ductal adenocarcinoma.

J Transl Med 2021 Aug 12;19(1):345. Epub 2021 Aug 12.

Department of Abdominal Oncology, Cancer Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

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http://dx.doi.org/10.1186/s12967-021-02997-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361738PMC
August 2021

Catalytic sulfate formation mechanism influenced by important constituents of cloud water the reaction of SO oxidized by hypobromic acid in marine areas.

Phys Chem Chem Phys 2021 Aug;23(30):15935-15949

Key Laboratory of Cluster Science, Ministry of Education of China, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Comprehensive investigations of the possible formation pathways of sulfate, the main composition of atmospheric aerosol in marine areas, continue to challenge atmospheric chemists. As one of the most important oxidation routes of S(iv) contributing to sulfate formation, the reaction process of S(iv) oxidized by hypobromic acid, which is ubiquitous with the gas-phase mixing ratios of ∼310 ppt and has a well-known oxidative capacity, has attracted wide attention. However, little information is available about the detailed reaction mechanism. Especially, due to the abundant species in cloud water, the potential effect of these compositions on these reaction processes and the corresponding effect mechanism are also uncertain. Using high-level quantum chemical calculations, we theoretically elucidate the two-step mechanism of Br+ transfer proposed by experiment through the verification of the key BrSO3- intermediate formation and subsequent hydrolysis reaction or the uncovered reaction of BrSO3- intermediate with OH-. Further, the novel and more competitive mechanisms (OH+ or O atom transfer pathways) that have not been considered in previous studies, leading to sulfate formation directly, have been found. Furthermore, it should be mentioned that we revealed the effect mechanism of constituents catalyzed in cloud water, especially the important H2O-catalyzed mechanism. In addition, all the above pathways follow this catalytic mechanism. This finding indicates a linkage between the complex nature of the atmospheric constituents and related atmospheric reaction, as well as the enhanced occurrence of atmospheric secondary sulfate formation in the atmosphere. Hence, this exploration of sulfate formation related to hypobromic acid could provide a better understanding about the sources of sulfate in marine areas.
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http://dx.doi.org/10.1039/d1cp01981cDOI Listing
August 2021

Application of constructed wetlands in treating rural sewage from source separation with high-influent nitrogen load: a review.

World J Microbiol Biotechnol 2021 Jul 19;37(8):138. Epub 2021 Jul 19.

National & Local Joint Engineering Research Center for Ecological Treatment Technology of Urban Water Pollution, Wenzhou University, Wenzhou, 325600, China.

Constructed wetlands (CWs) are characterized by low construction cost, convenient maintenance and management, and environmentally friendly features. They have emerged as promising technologies for decentralized sewage treatment across rural areas. Source separation of black water and gray water can facilitate sewage recycling and reuse of reclaimed water, reduce the size of treatment facilities, and lower infrastructure investment and operating cost. This is consistent with the concept of sustainable development. However, black water contains high concentrations of ammonia nitrogen, and the denitrification capacity of CWs is not excellent due to insufficient carbon source. Therefore, application of CWs for black water treatment faces challenges. This article provides a review on the progress in CWs for treatment of the sewage with high-influent nitrogen load, with emphasis on the commonly used strengthening means and the role of plants in nitrogen removal via CWs. The current issues of rural sewage treatment with high-influent nitrogen load by CWs are also assessed. Finally, the challenges and perspectives are discussed for the optimization of CWs-enhanced denitrification strategies.
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http://dx.doi.org/10.1007/s11274-021-03105-3DOI Listing
July 2021

Extracapsular subcutaneous endoscopic treatment for refractory lateral epicondylalgia: technique, retrospective results, and prognostic factors.

Ann Transl Med 2021 May;9(9):782

Institute of Sports Medicine, Peking University Third Hospital, Beijing, China.

Background: The extracapsular subcutaneous endoscopic treatment is a new and under-researched approach to the endoscopic treatment of refractory lateral epicondylalgia. We aimed to introduce the techniques of the method and the mid-long term clinical effects. Furthermore, we intended to identify demographic and surgical-related factors correlated with prognosis of extracapsular subcutaneous method.

Methods: Patients with a minimum of 6 months of conservative treatment for lateral epicondylalgia were recruited from March 2012 to July 2018. Key surgical techniques including microfracture and endoscopic suture were used. Visual analog scale (VAS), Mayo Elbow Performance Score (MEPS), and Disability of Arm (Shoulder and Hand, DASH) scores were used to evaluate clinical results. Univariable analysis and multivariable logistic regression were used to analyze effects of demographic characteristics and treatment techniques on prognosis.

Results: Seventy-eight patients were treated with modified subcutaneous extracapsular surgery. No serious complications such as neurovascular injury or infection occurred. Postoperative VAS, MEPS, and DASH scores significantly improved (P<0.001). Age was associated with prognosis regarding VAS score (P=0.023). Older age was an independent relevant factor for poor prognosis regarding VAS score (OR =0.914, 95% CI: 0.842-0.993, P=0.033), and microfracture during surgery related to poor prognosis for DASH score (OR =0.056, 95% CI: 0.004-0.783, P=0.032). Plaster fixation was an independent factor related to good prognosis regarding DASH score (OR =11.156, 95% CI: 1.009-123.363, P=0.049).

Conclusions: Extracapsular subcutaneous method of refractory lateral epicondylalgia has satisfactory and stable clinical results with high safety. Techniques of extracapsular method include debridement, microfracture, and tendon suture under endoscope. Young age, no microfracture, and postoperative plaster fixation were associated with good long-term prognosis.
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http://dx.doi.org/10.21037/atm-20-6799DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246197PMC
May 2021

The germline/somatic DNA damage repair gene mutations modulate the therapeutic response in Chinese patients with advanced pancreatic ductal adenocarcinoma.

J Transl Med 2021 07 12;19(1):301. Epub 2021 Jul 12.

Department of Abdominal Oncology, Cancer Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

Background: Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with molecular heterogeneity, inducing differences in biological behavior, and therapeutic strategy. NGS profiles of pathogenic alterations in the Chinese PDAC population are limited. We conducted a retrospective study to investigate the predictive role of DNA damage repair (DDR) mutations in precision medicine.

Methods: The NGS profiles were performed on resected tissues from 195 Chinese PDAC patients. Baseline clinical or genetic characteristics and survival status were collected. The Kaplan-Meier survival analyses were performed by the R version 3.6.1.

Results: The main driver genes were KRAS, TP53, CDKN2A, and SMAD4. Advanced patients with KRAS mutation showed a worse OS than KRAS wild-type (p = 0.048). DDR pathogenic deficiency was identified in 30 (15.38%) of overall patients, mainly involving BRCA2 (n = 9, 4.62%), ATM (n = 8, 4.10%) and RAD50 genes (n = 3, 1.54%). No significance of OS between patients with or without DDR mutations (p = 0.88). But DDR mutation was an independent prognostic factor for survival analysis of advanced PDAC patients (p = 0.032). For DDR mutant patients, treatment with platinum-based chemotherapy (p = 0.0096) or olaparib (p = 0.018) respectively improved the overall survival. No statistical difference between tumor mutation burden (TMB) and DDR mutations was identified. Treatment of PD-1 blockades did not bring significantly improved OS to DDR-mutated patients than the naive DDR group (p = 0.14).

Conclusions: In this retrospective study, we showed the role of germline and somatic DDR mutation in predicting the efficacy of olaparib and platinum-based chemotherapy in Chinese patients. However, the value of DDR mutation in the prediction of hypermutation status and the sensitivity to the PD-1 blockade needed further investigation.
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http://dx.doi.org/10.1186/s12967-021-02972-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273977PMC
July 2021

Rewiring the microbial metabolic network for efficient utilization of mixed carbon sources.

J Ind Microbiol Biotechnol 2021 Jul 2. Epub 2021 Jul 2.

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.

Carbon sources represent the most dominant cost factor in the industrial biomanufacturing of products. Thus, it has attracted much attention to seek cheap and renewable feedstocks, such as lignocellulose, crude glycerol, methanol and carbon dioxide, for biosynthesis of value-added compounds. Co-utilization of those carbon sources by microorganisms not only can reduce the production cost but also serves as a promising approach to improve the carbon yield. However, co-utilization of mixed carbon sources usually suffers from a low utilization rate. In the past few years, the development of metabolic engineering strategies to enhance carbon source co-utilization efficiency by inactivation of carbon catabolite repression has made significant progress. In this article, we provide informative and comprehensive insights into the co-utilization of two or more carbon sources including glucose, xylose, arabinose, glycerol and C1 compounds, and we put our focus on parallel utilization, synergetic utilization and complementary utilization of different carbon sources. Our goal is not only to summarize strategies of co-utilization of carbon sources, but also to discuss how to improve the carbon yield and the titer of target products.
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http://dx.doi.org/10.1093/jimb/kuab040DOI Listing
July 2021

Beijing Friendship Hospital Osteoporosis Self-Assessment Tool for Elderly Male (BFH-OSTM) vs Fracture Risk Assessment Tool (FRAX) for identifying painful new osteoporotic vertebral fractures in older Chinese men: a cross-sectional study.

BMC Musculoskelet Disord 2021 Jun 28;22(1):596. Epub 2021 Jun 28.

Department of Orthopedics, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.

Background: To compare the validation of four tools for identifying painful new osteoporotic vertebral compression fractures (PNOVCFs) in older Chinese men: bone mineral density (BMD), Asian osteoporosis self-assessment tool (OSTA), World Health Organization fracture risk assessment tool (FRAX) (without BMD) and Beijing Friendship Hospital Osteoporosis Self-Assessment Tool (BFH-OSTM).

Methods: A cross sectional study was conducted from 2013 to 2019. A total of 846 men aged ≥50 were included and were divided into two groups: Fracture Group (patients with PNOVCFs underwent percutaneous vertebroplasty surgery) and Non-Fracture Group (community dwelled subjects for healthy examination). All subjects accepted a dual-energy X-ray BMD test and a structured questionnaire. The results of BMD, OSTA, FRAX and BFH-OSTM scores were assessed and receiver-operating characteristic (ROC) curves were generated to compare the validity of four tools for identifying PNOVCFs. Optimal cutoff points, sensitivity, specificity, and areas under the ROC curves (AUCs) were determined.

Results: There were significant differences including BMD T score (femoral neck, total hip and L1-L4), OSTA, FRAX and BFH-OSTM scores between Fracture group and Non-fracture group. Compared to BMD and OSTA, BFH-OSTM and FRAX had better predictive value, the sensitivity, specificity and AUC value are 0.841, 81.29%, 70.67% and 0.796, 74.85%, 78.52%, respectively. Compared with FRAX, the BFH-OSTM has a better AUC value.

Conclusions: Both BFH-OSTM and FRAX can be used to identify POVCFs, However, BFH-OSTM model may be a more simple and effective tool to identify the risk of POVCFs in Chinese elderly men.
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http://dx.doi.org/10.1186/s12891-021-04476-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240203PMC
June 2021

Team Science in Precision Medicine: Study of Coleadership and Coauthorship Across Health Organizations.

J Med Internet Res 2021 06 14;23(6):e17137. Epub 2021 Jun 14.

Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States.

Background: Interdisciplinary collaborations bring lots of benefits to researchers in multiple areas, including precision medicine.

Objective: This viewpoint aims at studying how cross-institution team science would affect the development of precision medicine.

Methods: Publications of organizations on the eHealth Catalogue of Activities were collected in 2015 and 2017. The significance of the correlation between coleadership and coauthorship among different organizations was calculated using the Pearson chi-square test of independence. Other nonparametric tests examined whether organizations with coleaders publish more and better papers than organizations without coleaders.

Results: A total of 374 publications from 69 organizations were analyzed in 2015, and 7064 papers from 87 organizations were analyzed in 2017. Organizations with coleadership published more papers (P<.001, 2015 and 2017), which received higher citations (Z=-13.547, P<.001, 2017), compared to those without coleadership. Organizations with coleaders tended to publish papers together (P<.001, 2015 and 2017).

Conclusions: Our findings suggest that organizations in the field of precision medicine could greatly benefit from institutional-level team science. As a result, stronger collaboration is recommended.
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http://dx.doi.org/10.2196/17137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240795PMC
June 2021

LINC00943 is correlated with gastric cancer and regulates cancer cell proliferation and chemosensitivity via hsa-miR-101-3p.

Int J Clin Oncol 2021 Sep 4;26(9):1650-1660. Epub 2021 Jun 4.

Department of Endocrinology, Rizhao People's Hospital, Rizhao, 276800, Shandong Province, China.

Background: Non-coding RNAs have emerged as important regulators in human cancers. In this work, we investigated the role of long intergenic non-protein-coding RNA 943 (LINC00943) in gastric cancer (GC).

Methods: LINC00943 expression was evaluated in GC patient tissues and cell lines. In SNU-5 and MKN-45 cells, LINC00943 was knocked down to investigate its roles in regulating GC cell proliferation, 5-FU chemosensitivity and in vivo explant growth. Possible downstream target of LINC00943, human mature microRNA-101-3p (hsa-miR-101-3p) was also evaluated.

Results: LINC00943 was aberrantly overexpressed in in situ GC tumors and immortal GC cell lines. LINC00943 overexpression was associated with GC patients' poor prognosis. LINC00943 knockdown reduced GC cell proliferation, 5-FU resistance and in vivo explant growth. Hsa-miR-101-3p was found to be regulated by LINC00943 in GC. Hsa-miR-101-3p downregulation reversed the tumor-suppressing functions of LINC00943 knockdown in GC cells.

Conclusion: In summary, our results indicated that LINC00943 was correlated with gastric cancer and regulates cancer cell proliferation and chemosensitivity via hsa-miR-101-3p.
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http://dx.doi.org/10.1007/s10147-021-01945-5DOI Listing
September 2021

Enhanced visible-light-driven photocatalytic activity of bismuth oxide via the decoration of titanium carbide quantum dots.

J Colloid Interface Sci 2021 Oct 8;600:161-173. Epub 2021 May 8.

College of Environmental Science and Engineering, Hunan University, Changsha, Hunan 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, China.

In TiC quantum dots (TiC QDs)/BiO photocatalysts system, TiC QDs can act as a co-catalyst to greatly boost the photocatalytic performance of BiO. TiC QDs with excellent light adsorption ability can improve the light response of the system, and the fascinating electronic property can function as a channel for electron transfer. Moreover, TiC QDs possess larger specific area and more active edge atoms thanks to the size effect. The best TiC QDs/BiO composite with the loading amounts of 75 mL of TiC QDs solution showed much higher photocatalytic performance (nearly 5.85 times) for tetracycline (TC) degradation than that of pristine BiO under visible light irradiation. These different photocatalytic performances shed light on the key role of TiC QDs in stimulating the photocatalytic activity of BiO. Moreover, TiC QDs/BiO composites exhibited excellent stability in recycling experiments and actual water sample treatment.
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http://dx.doi.org/10.1016/j.jcis.2021.05.022DOI Listing
October 2021

A pH dependent sulfate formation mechanism caused by hypochlorous acid in the marine atmosphere.

Sci Total Environ 2021 Sep 14;787:147551. Epub 2021 May 14.

Key Laboratory of Cluster Science, Ministry of Education of China, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China. Electronic address:

Secondary sulfate plays a crucial role in forming marine aerosol, which in turn is an important source of natural aerosol at a global level. Recent experimental studies suggest that oxidation of S(IV) compounds, in practice dissolved sulfur dioxide, to sulfate (S(VI)) by hypochloric acid could be one of the most significant pathways for sulfate formation in marine areas. However, the exact mechanism responsible for this process remains unknown. Using high-level quantum chemical calculations, we studied the reaction between dissolved sulfur dioxide and hypochloric acid. We account for the dominant protonation states of reactants in the pH range 3.0-9.0. We also consider possible catalytic effects of species such as HO. Our results show that sulfate formation in HOCl+HOSO and HOCl+SO reactions relevant to acidic and nearly neutral conditions can occur either through previously proposed Cl transfer or through a novel HO transfer mechanism. In alkaline conditions, where the dominant reactants are OCl and SO, an O atom transfer mechanism proposed in previous experimental studies may be more important than Cl transfer. Catalysis by common cloud-water species is found to lower barriers of Cl transfer mechanisms substantially. Nevertheless, we find that the dominant S(IV) + HOCl reaction mechanism for the full studied pH range is HO transfer from HOCl to SO, which leads directly to sulfate formation without ClSO intermediates. The rate-limiting barrier of this reaction is low, leading to an essentially diffusion-controlled reaction rate. S(IV) lifetimes due to this reaction decrease with increasing pH due to the increasing fractional population of SO. Especially in neutral and alkaline conditions, depletion of HOCl by the reaction is so rapid that S(IV) oxidation will be controlled mainly by mass transfer of gas-phase HOCl to the liquid phase. The mechanism proposed here may help to explain marine sulfate sources missing from current atmospheric models.
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http://dx.doi.org/10.1016/j.scitotenv.2021.147551DOI Listing
September 2021

Polyploidy underlies co-option and diversification of biosynthetic triterpene pathways in the apple tribe.

Proc Natl Acad Sci U S A 2021 May;118(20)

Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;

Whole-genome duplication (WGD) plays important roles in plant evolution and function, yet little is known about how WGD underlies metabolic diversification of natural products that bear significant medicinal properties, especially in nonmodel trees. Here, we reveal how WGD laid the foundation for co-option and differentiation of medicinally important ursane triterpene pathway duplicates, generating distinct chemotypes between species and between developmental stages in the apple tribe. After generating chromosome-level assemblies of a widely cultivated loquat variety and we define differentially evolved, duplicated gene pathways and date the WGD in the apple tribe at 13.5 to 27.1 Mya, much more recent than previously thought. We then functionally characterize contrasting metabolic pathways responsible for major triterpene biosynthesis in and loquat, which pre- and postdate the Maleae WGD, respectively. Our work mechanistically details the metabolic diversity that arose post-WGD and provides insights into the genomic basis of medicinal properties of loquat, which has been used in both traditional and modern medicines.
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http://dx.doi.org/10.1073/pnas.2101767118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157987PMC
May 2021

Cyclosporine A blocks autophagic flux in tubular epithelial cells by impairing TFEB-mediated lysosomal function.

J Cell Mol Med 2021 06 4;25(12):5729-5743. Epub 2021 May 4.

Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City, Institute of Nephrology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.

Cyclosporine A (CsA) is an immunosuppressor widely used for the prevention of acute rejection during solid organ transplantation. However, severe nephrotoxicity has substantially limited its long-term usage. Recently, an impaired autophagy pathway was suggested to be involved in the pathogenesis of chronic CsA nephrotoxicity. However, the underlying mechanisms of CsA-induced autophagy blockade in tubular cells remain unclear. In the present study, we observed that CsA suppressed the activation and expression of transcription factor EB (TFEB) by increasing the activation of mTOR, in turn promoting lysosomal dysfunction and autophagy flux blockade in tubular epithelial cells (TECs) in vivo and in vitro. Restoration of TFEB activation by Torin1-mediated mTOR inhibition significantly improved lysosomal function and rescued autophagy pathway activity, suppressing TEC injury. In summary, targeting TFEB-mediated autophagy flux represents a potential therapeutic strategy for CsA-induced nephrotoxicity.
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http://dx.doi.org/10.1111/jcmm.16593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184677PMC
June 2021

Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration.

Bioengineered 2021 12;12(1):1299-1310

Department of Gynecology, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Differentiated thyroid carcinoma (DTC) is one of the most common malignant tumors. Increasing evidence indicates that centromere protein K(CENPK) may play a key role in promoting carcinogenesis. The expression, biological functions, and clinical significance of CENPK in DTC are still unclear. The CENPK expression in the DTC specimen was confirmed using quantitative real-time PCR and Western blot. The expression of CENPK was silenced and promoted by lentivirus-mediated transfection with shRNA sequences or CENPK plasmid targeting CENPK in TPC1 and FTC-133 cells, respectively. Colony formation, Cell Counting Kit-8 (CCK-8), Transwell invasion, and scratch assays were performed to assess the malignant biological properties of FTC-133 and TPC1 cells. Tumorigenicity assay was performed using C57BL/6 mice to explore the influence of CENPK on the growth of TPC1. The present work suggested that the expression of CENPK remarkably increased in follicular thyroid cancer and papillary thyroid cancer  tissue samples at the mRNA level. Immunohistochemical staining also showed consistent results at the protein level. In addition, CENPK mRNA expression level showed great value in diagnosis of DTC. Knockdown of CENPK significantly inhibited the invasion and migration of TPC1 and FTC-133 cells. In contrast, CENPK overexpression promoted invasion and migration of TPC1 and FTC-133 cells. Knockdown and overexpression of CENPK showed consistent effect on DTC tumor growth and expression of Ki-67 invivo. Our results indicated that CENPK was evidently upregulated in DTC. Knocking down CENPK suppressed TPC1 cell proliferation, invasion and migration. Targeting the CENPK may be anovel therapeutic method for DTC.
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http://dx.doi.org/10.1080/21655979.2021.1911533DOI Listing
December 2021

Predicting successful stellate ganglion block using laser speckle contrast imaging.

Chin Med J (Engl) 2021 Apr 7;134(12):1486-1488. Epub 2021 Apr 7.

Department of Anesthesiology, Institute of Anesthesiology and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.

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http://dx.doi.org/10.1097/CM9.0000000000001398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213298PMC
April 2021

Kilometers Long Graphene-Coated Optical Fibers for Fast Thermal Sensing.

Research (Wash D C) 2021 18;2021:5612850. Epub 2021 Mar 18.

Key Laboratory of Optical Fiber Sensing and Communications (Education Ministry of China), University of Electronic Science and Technology of China, Chengdu 611731, China.

The combination of optical fiber with graphene has greatly expanded the application regimes of fiber optics, from dynamic optical control and ultrafast pulse generation to high precision sensing. However, limited by fabrication, previous graphene-fiber samples are typically limited in the micrometer to centimeter scale, which cannot take the inherent advantage of optical fibers-long-distance optical transmission. Here, we demonstrate kilometers long graphene-coated optical fiber (GCF) based on industrial graphene nanosheets and coating technique. The GCF shows unusually high thermal diffusivity of 24.99 mm s in the axial direction, measured by a thermal imager directly. This enables rapid thermooptical response both in optical fiber Bragg grating sensors at one point (18-fold faster than conventional fiber) and in long-distance distributed fiber sensing systems based on backward Rayleigh scattering in optical fiber (15-fold faster than conventional fiber). This work realizes the industrial-level graphene-fiber production and provides a novel platform for two-dimensional material-based optical fiber sensing applications.
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http://dx.doi.org/10.34133/2021/5612850DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8000361PMC
March 2021

NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.

Clin Transl Med 2021 03;11(3):e373

Department of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.

Background: Blood transfusion, a common basic supporting therapy, can lead to acute hemolytic transfusion reaction (AHTR). AHTR poses a great risk to patients through kidney function damage in a short time. Previous reports found that heme from destroyed red blood cells impaired kidney function, and NLR family pyrin domain containing 3 (NLRP3) inflammasome was augmented in case of kidney injury. However, the detailed mechanism regarding whether NLRP3 inflammasome is involved in kidney function injury in AHTR is not fully understood yet.

Methods: Hemolysis models were established by vein injection with human blood plasma or mouse heme from destroyed red blood cells. The injured renal tubular epithelial cells (RTECs) were evaluated by tubular damage markers staining in hemolysis models and in primary RTECs in vitro. The activation of NLRP3 inflammasome in RTECs by hemes was investigated by Western blot, ELISA, scanning electron microscopy, immunofluorescent staining, flow cytometry, and hemolysis models. NLRP3 gene knockout mice were employed to confirm these observations in vitro and in vivo. The binding between a novel inhibitor (66PR) and NLRP3 was affirmed by molecule docking and co-immunoprecipitation. The rescue of 66PR on kidney function impairment was explored in murine hemolysis models.

Results: We found that heme could activate NLRP3 inflammasome in RTECs to induce kidney function injury. NLRP3 gene knockout could prevent the damage of RTECs caused by hemes and recover kidney function in AHTR. Moreover, NLRP3 inflammasome chemical inhibitor, 66PR, could bind to NLRP3 protein and inhibit inflammasome activation in RTECs, which consequently relieved the injury of RTECs caused by hemes, and alleviated kidney function damage in the AHTR model.

Conclusions: Hemes could activate NLRP3 inflammasome in RTECs, and a novel NLRP3 inflammasome inhibitor named 66PR relieved kidney function damage in AHTR. Our findings provided a new possible strategy to treat kidney function failure in AHTR.
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http://dx.doi.org/10.1002/ctm2.373DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009139PMC
March 2021

Association between preoperative serum TSH and tumor status in patients with papillary thyroid microcarcinoma.

Endocrine 2021 09 23;73(3):617-624. Epub 2021 Mar 23.

Department of Head and Neck Surgery, Gansu Provincial Cancer Hospital, Lanzhou, 730050, China.

Thyroid-stimulating hormone (TSH) is a growth factor affecting the initiation or progression of papillary thyroid cancer (PTC). However, the relationship between preoperative serum TSH and papillary thyroid microcarcinoma (PTMC) remains controversial. To investigate the relationship between preoperative serum TSH and tumor status of PTMC, a multicentered retrospective study was performed from January 2014 to December 2016. The cohort of this study consisted of 1997 patients who underwent thyroid surgery. Serum TSH concentrations were measured and PTMC was diagnosed based on the post-operation pathological report. Results showed that the preoperative serum TSH concentration was not related to age and gender but was positively associated with tumor size. Furthermore, higher TSH level was associated with extra-thyroidal extension and lymph node metastasis (LNM). These results indicated that TSH might not be involved in the development of PTMC but may be associated with PTMC progression. Preoperative serum TSH concentration should be considered as risk predictor for tumor progression in patients with PTMC.
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http://dx.doi.org/10.1007/s12020-021-02690-5DOI Listing
September 2021

Building the mental health management system for children post COVID-19 pandemic: an urgent focus in China.

Eur Child Adolesc Psychiatry 2021 Mar 23. Epub 2021 Mar 23.

Department of Psychiatry, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

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http://dx.doi.org/10.1007/s00787-021-01763-0DOI Listing
March 2021

Adsorption and visible-light photocatalytic degradation of organic pollutants by functionalized biochar: Role of iodine doping and reactive species.

Environ Res 2021 06 17;197:111026. Epub 2021 Mar 17.

School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China; City University of Hong Kong Shenzhen Research Institute, 8 Yuexing 1st Road, Shenzhen Hi-Tech Industrial Park, Nanshan District, Shenzhen, 518060, China. Electronic address:

Here we developed the functionalized biochar as low-cost and heavy metal-free photocatalysts via a facile iodine doping method, which exhibit efficient adsorption and visible-light-driven photocatalytic degradation of representative organic pollutants, phenol and tetracycline. On one hand, iodine doping elevates the adsorption via creating extra pores, e.g., the adsorbed amounts of phenol by iodine-doped WSP and OSR biochar are increased by 161.8% and 146.3%, respectively, which in turn facilitates the photocatalytic oxidation of the adsorbed pollutants. On the other hand, iodine doping leads to the strong photo-induced excitation and remarkably reduced charge carrier transfer resistance, boosting the photocatalytic activity of iodine-doped biochar by more than 20 orders towards organic pollutants (e.g., phenol) degradation. The systematic analysis of reactive species reveals the active roles of O, HO, O, OH, electrons, and holes in photocatalytic process and identifies O to be the major contributor. This work affords a facile approach to generating porous and visible-light-driven photocatalyst from biomass for efficient adsorbing and degrading organic pollutants, opening up an avenue to turn biowaste into biomaterials for sustainable environmental remediation.
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http://dx.doi.org/10.1016/j.envres.2021.111026DOI Listing
June 2021

bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway.

Redox Biol 2021 05 20;41:101904. Epub 2021 Feb 20.

School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, 325000, PR China. Electronic address:

Protein S-nitrosylation is a reversible protein modification implicated in both physiological and pathophysiological regulation of protein function. However, the relationship between dysregulated S-nitrosylation homeostasis and diabetic vascular complications remains incompletely understood. Here, we demonstrate that basic fibroblast growth factor (bFGF) is a key regulatory link between S-nitrosylation homeostasis and inflammation, and alleviated endothelial dysfunction and angiogenic defects in diabetes. Subjecting human umbilical vein endothelial cells (HUVECs) to hyperglycemia and hyperlipidemia significantly decreased endogenous S-nitrosylated proteins, including S-nitrosylation of inhibitor kappa B kinase β (IKKβ) and transcription factor p65 (p65), which was alleviated by bFGF co-treatment. Pretreatment with carboxy-PTIO (c-PTIO), a nitric oxide scavenger, abolished bFGF-mediated S-nitrosylation increase and endothelial protection. Meanwhile, nitrosylation-resistant IKKβ and p65 mutants exacerbated endothelial dysfunction in db/db mice, and in cultured HUVECs subjected to hyperglycemia and hyperlipidemia. Mechanistically, bFGF-mediated increase of S-nitrosylated IKKβ and p65 was attributed to synergistic effects of increased endothelial nitric oxide synthase (eNOS) and thioredoxin (Trx) activity. Taken together, the endothelial protective effect of bFGF under hyperglycemia and hyperlipidemia can be partially attributed to its role in suppressing inflammation via the S-nitrosylation pathway.
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http://dx.doi.org/10.1016/j.redox.2021.101904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972985PMC
May 2021

Using Machine Learning Technologies in Pressure Injury Management: Systematic Review.

JMIR Med Inform 2021 Mar 10;9(3):e25704. Epub 2021 Mar 10.

Key Laboratory of Knowledge Engineering with Big Data of the Ministry of Education, School of Computer Science and Information Engineering, Hefei University of Technology, Hefei, China.

Background: Pressure injury (PI) is a common and preventable problem, yet it is a challenge for at least two reasons. First, the nurse shortage is a worldwide phenomenon. Second, the majority of nurses have insufficient PI-related knowledge. Machine learning (ML) technologies can contribute to lessening the burden on medical staff by improving the prognosis and diagnostic accuracy of PI. To the best of our knowledge, there is no existing systematic review that evaluates how the current ML technologies are being used in PI management.

Objective: The objective of this review was to synthesize and evaluate the literature regarding the use of ML technologies in PI management, and identify their strengths and weaknesses, as well as to identify improvement opportunities for future research and practice.

Methods: We conducted an extensive search on PubMed, EMBASE, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Cochrane Library, China National Knowledge Infrastructure (CNKI), the Wanfang database, the VIP database, and the China Biomedical Literature Database (CBM) to identify relevant articles. Searches were performed in June 2020. Two independent investigators conducted study selection, data extraction, and quality appraisal. Risk of bias was assessed using the Prediction model Risk Of Bias ASsessment Tool (PROBAST).

Results: A total of 32 articles met the inclusion criteria. Twelve of those articles (38%) reported using ML technologies to develop predictive models to identify risk factors, 11 (34%) reported using them in posture detection and recognition, and 9 (28%) reported using them in image analysis for tissue classification and measurement of PI wounds. These articles presented various algorithms and measured outcomes. The overall risk of bias was judged as high.

Conclusions: There is an array of emerging ML technologies being used in PI management, and their results in the laboratory show great promise. Future research should apply these technologies on a large scale with clinical data to further verify and improve their effectiveness, as well as to improve the methodological quality.
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http://dx.doi.org/10.2196/25704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991995PMC
March 2021

Atypical enhanced computed tomography signs of pancreatic cancer and its differential diagnosis from autoimmune pancreatitis.

Gland Surg 2021 Jan;10(1):347-354

Department of Radiology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.

Background: To analyze the atypical enhanced computed tomography (CT) signs of pancreatic cancer (PC) and compare them with those of autoimmune pancreatitis (AIP) to explore the differential diagnosis value of CT.

Methods: The clinical data of 36 AIP (AIP group) and 38 PC patients (PC group), who were admitted to our hospital from January 2013 to June 2020 and confirmed by surgical biopsy or hormone therapy, were retrospectively analyzed. Participants in both groups were examined by CT, the imaging signs of the 2 groups were analyzed, and the results of CT examination were compared.

Results: In the PC group, the density of the lesions on the CT scan was mostly reduced, the pancreas was not swollen, and the kidneys were not involved. The bile duct wall was thickened with a sausage-like appearance, enveloped edges were rare, blood vessels were invaded, lymph nodes were enlarged, and the pancreatic duct was truncated. The findings of the AIP group were the opposite. The difference in the proportion of participants with the above-mentioned CT features between the 2 groups was statistically significant (P<0.05). The shape of the lesions in the AIP group was mainly elongated, of uneven density, and the density of enhanced scanning was medium to high. The predominant shape of the lesions in PC participants was spherical, and the density was uniform. The enhanced scan was mainly low-density. The difference in shape and density between the 2 groups was also statistically significant (P<0.05). The CT values of the plain scan, intravenous phase, and delayed phase in the AIP group were significantly higher than those in the PC group (P<0.05).

Conclusions: The imaging signs of AIP and PC overlap. Examination with CT is of great value in the differential diagnosis between AIP and PC. Familiarity with and mastery of the CT signs of AIP and PC can help to improve the accuracy of clinical diagnosis and provide a reliable basis for patients' follow-up treatment.
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http://dx.doi.org/10.21037/gs-20-821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882330PMC
January 2021

Enhancing iron redox cycling for promoting heterogeneous Fenton performance: A review.

Sci Total Environ 2021 Jun 14;775:145850. Epub 2021 Feb 14.

College of Environmental Science and Engineering, Hunan University, Changsha, Hunan 410082, China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, China.

Conventional water treatment methods are difficult to remove stubborn pollutants emerging from surface water. Advanced oxidation processes (AOPs) can achieve a higher level of mineralization of stubborn pollutants. In recent years, the Fenton process for the degradation of pollutants as one of the most efficient ways has received more and more attention. While homogeneous catalysis is easy to produce sludge and the catalyst cannot be cycled. In contrast, heterogeneous Fenton-like reaction can get over these drawbacks and be used in a wider range. However, the reduction of Fe (III) to Fe(II) by hydrogen peroxide (HO) is still the speed limit step when generating reactive oxygen species (ROS) in heterogeneous Fenton system, which restricts the efficiency of the catalyst to degrade pollutants. Based on previous research, this article reviews the strategies to improve the iron redox cycle in heterogeneous Fenton system catalyzed by iron materials. Including introducing semiconductor, the modification with other elements, the application of carbon materials as carriers, the introduction of metal sulfides as co-catalysts, and the direct reduction with reducing substances. In addition, we also pay special attention to the influence of the inherent properties of iron materials on accelerating the iron redox cycle. We look forward that the strategy outlined in this article can provide readers with inspiration for constructing an efficient heterogeneous Fenton system.
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http://dx.doi.org/10.1016/j.scitotenv.2021.145850DOI Listing
June 2021

Effect of Long-Term Fertilization on Ammonia-Oxidizing Microorganisms and Nitrification in Brown Soil of Northeast China.

Front Microbiol 2020 4;11:622454. Epub 2021 Feb 4.

College of Land and Environment, Shenyang Agricultural University, Shenyang, China.

The objective of this study was to find out changes in ammonia oxidation microorganisms with respect to fertilizer as investigated by real-time polymerase chain reaction and high-throughput sequencing. The treatments included control (CK); chemical fertilizer nitrogen low (N) and high (N); nitrogen and phosphorus (NP); nitrogen phosphorus and potassium (NPK) and organic manure fertilizer (M); MN; MN; MNPK. The results showed that long-term fertilization influenced soil fertility and affected the abundance and community of ammonia-oxidizing microorganisms by changing the physical and chemical properties of the soil. The abundance and community structure of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) was influenced by soil organic carbon, total nitrogen, total soil phosphorus, available phosphorus, available potassium, and soil nitrate. Soil environmental factors affected the nitrification potential by affecting the structure of ammonia-oxidizing microorganisms; specific and rare AOA and AOB rather than the whole AOA or AOB community played dominant role in nitrification.
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http://dx.doi.org/10.3389/fmicb.2020.622454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890093PMC
February 2021
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