Publications by authors named "Nicolás Farnetano"

2 Publications

  • Page 1 of 1

Eicosapentaenoic acid supplemented to in vitro maturation medium results in lesser lipid content and intracellular reactive oxygen species in blastocysts of cattle.

Anim Reprod Sci 2021 Jun 9;229:106765. Epub 2021 May 9.

IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N Dulout"(UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP, Calles 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina. Electronic address:

Sub-optimal cattle embryo development to the blastocyst stage still is a problem when conducting in vitro production (IVP) procedures. Supplementation of in vitro maturation (IVM) medium with omega 3-polyunsaturated eicosapentaenoic acid (EPA) is an approach that might have positive effects on lipid metabolism of cattle oocytes, potentially improving subsequent embryo development. The aim of this study was to evaluate effects of EPA addition to serum-free IVM medium on pronuclear formation after in vitro fertilization, cleavage, and blastocyst rates. Effects of EPA on lipid accumulation and intracellular reactive oxygen species (ROS) generation with IVP of cattle embryos was also investigated. In all experiments, cumulus-oocyte complexes were matured in IVM medium supplemented with 0 nM, 1 nM, or 1 μM EPA for 24 h. Pronuclear formation, cleavage, and blastocyst rates were similar for embryos when there was supplementation of EPA at all concentrations to those of the control group (P > 0.05). The inclusion of 1 nM EPA in medium resulted in a greater lipid content and less intracellular ROS in day 8-embryos compared with those of the Control group (P < 0.05). There were no differences, however, when there was inclusion of 1 μM EPA compared to embryos of the Control group at the day 8 developmental stage (P > 0.05). In conclusion, supplementation with IVM medium with the 1 nM EPA concentration resulted in a lesser blastocyst lipid and intracellular ROS concentration, without modifying embryo development, therefore, EPA could be a desirable supplement to improve embryo quality in cattle.
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http://dx.doi.org/10.1016/j.anireprosci.2021.106765DOI Listing
June 2021

Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes.

Biol Trace Elem Res 2020 Sep 8;197(1):149-158. Epub 2019 Nov 8.

IGEVET - Instituto de Genética Veterinaria "Ing. Fernando N. Dulout" (UNLP-CONICET LA PLATA) Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118s/n, CP 1900, La Plata, Buenos Aires, Argentina.

Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus-oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.
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http://dx.doi.org/10.1007/s12011-019-01966-2DOI Listing
September 2020