Publications by authors named "Nicholas Richwagen"

2 Publications

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Transmission frequency of COVID-19 through pre-symptomatic and asymptomatic patients in AJK: a report of 201 cases.

Virol J 2021 07 3;18(1):138. Epub 2021 Jul 3.

College of Medicine, University of Central Florida, Florida, USA.

Background: The COVID-19 pandemic is a catastrophic global phenomenon, affecting human life in a way unseen since the 1918 influenza pandemic. Effective management of this threat requires halting transmission, a strategy requiring accurate knowledge of SARS-CoV-2 transmission patterns.

Methods: This was a retrospective contact study aiming to estimate the transmission rate of COVID-19 by tracing contacts in symptomatic, pre-symptomatic, and asymptomatic patients. History of patients' contacts during 24 h before appearance of symptoms or infection confirmation was traced for disease transmission.

Results: Overall, a total of 201 COVID-19 patients had contact with 7168 people in 24 h with an average of 35.66 contacts per patient, ranging from a minimum of 4 to maximum of 87 contacts (meetings). Out of 7168 persons met, infection was detected in 64 (0.89%). For the 155 symptomatic patients, a total of 5611 contacted persons were traced before appearance of symptoms (pre-symptomatic) in last 24 h with an average of 36.20 meetings per patient. The infection was transmitted in 63 (1.12%) people with 5548 (98.88%) remaining uninfected. Out of the 63 transmissions, 62 (98.4%) were traced within 6 h before symptom onset, while only 1 was identified in the 6-12 h timeframe before symptoms. A total of 1557 persons were traced having meeting/contacts with asymptomatic cases in last 24 h before infection confirmation. Out of these 1557 persons, only 1 was found to be infected and the infection rate was calculated to be 0.06%. Statistically, the transmission rate by pre-symptomatic patients was found to be significantly higher than the transmission rate by asymptomatic individuals (P < 0.05).

Conclusion: In the studied population, the risk of pre-symptomatic and asymptomatic transmission of COVID-19 was low, with transmission risks of 1.12% and 0.06% respectively. Pre-symptomatic infection becomes very rare in contacts made longer than 6 h before onset of symptoms. The infection transmission is traced as long as about 9 h before the appearance of clear symptoms in the patients, but the incidence rate was as low as about 0.02% of the total contacts in that period.
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http://dx.doi.org/10.1186/s12985-021-01609-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254450PMC
July 2021

Antibacterial Activity of and Against ESKAPE Pathogens.

Front Pharmacol 2019 6;10:67. Epub 2019 Feb 6.

Center for the Study of Human Health, Emory College of Arts and Sciences, Atlanta, GA, United States.

Plants in the genus (Family: Crassulaceae) are used in traditional medicine throughout the tropics for treating a variety of conditions. Two species, and , have established ethnobotanical usage but have been neglected in previous research concerning their potential bioactivity. Here, we provide a thorough review of the reported antimicrobial activities of genus and evaluate the antibacterial effects of two previously unexplored species against a panel of multidrug-resistant bacteria, the ESKAPE pathogens (, and ). Plant specimens were collected and voucher specimens deposited in the Emory University Herbarium. Dried plant material was ground into a powder and extracted as ethanolic macerations or as aqueous decoctions. Extracts were tested against the ESKAPE pathogens for growth inhibitory activity. Cytotoxicity to human cells was assessed via a lactate dehydrogenase assay of treated human keratinocytes (HaCaTs). extracts demonstrated growth inhibitory effects against two Gram-negative species, (strain CDC-33) and (AH-71), as well as (UAMS-1). In these cases, growth inhibition greater than 50% (IC) was generally observed at concentrations of 256 μg mL, though one extract (1465, prepared from stems) exhibited an IC against at 128 μg mL. All extracts were well tolerated by HaCaTs (LD ≥ 256 μg mL). Chemical characterization using HPLC and chemical standards established the presence of caffeic acid and quercetin in both plant species, as well as kaempferol in These results reveal to be a plant of medicinal interest, and future research should aim to characterize the bioactivity of this species and its active constituents through bioassay-guide fractionation. Effects on bacterial biofilm formation and quorum-sensing are also research topics of interest for this genus.
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http://dx.doi.org/10.3389/fphar.2019.00067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374630PMC
February 2019
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