Publications by authors named "Nezafat Firizi Maryam"

3 Publications

  • Page 1 of 1

Cytokine profile and nitric oxide levels in macrophages exposed to Leishmania infantum FML.

Exp Parasitol 2019 Aug 22;203:1-7. Epub 2019 May 22.

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran; Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.

Fucose-mannose ligand (FML) is a soluble antigen purified from Leishmania donovani complex and used for diagnosis, prognosis, and vaccine development against visceral leishmaniasis (VL). We aimed to explore the effects of FML on the production of cytokines, chemokines and nitric oxide (NO) by macrophages in vitro. Peritoneal macrophages from BALB/c mice were treated with various concentrations of FML purified from Leishmania infantum in the absence or presence of LPS Peritoneal macrophages. After 48hr, cell culture supernatants were recovered and the levels of TNF-α, IL-10, IL-12p70 and IP-10 measured by Sandwich ELISA and NO concentration by Griess reaction. We found that FML significantly increase NO, IL-12p70 and IP-10 production in both LPS-treated and untreated macrophages and increase IL-10 levels only in LPS-treated macrophages. However, FML could not alert TNF-α levels in both LPS-treated and untreated macrophages. Further analysis revealed that FML can also increase IL-12p70/IL-10 ratio in LPS-treated macrophages. We concluded that FML can polarize macrophages to an appropriate phenotype similar to M1 phenotype against Leishmania donovani complex, although IL10 and TNF results are controversial.
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http://dx.doi.org/10.1016/j.exppara.2019.05.004DOI Listing
August 2019

The protective effect of berberine against lipopolysaccharide-induced abortion by modulation of inflammatory/immune responses.

Immunopharmacol Immunotoxicol 2018 Aug 13;40(4):333-337. Epub 2018 Jul 13.

d Department of Pathobiology and Laboratory Sciences , North Khorasan University of Medical Sciences , Bojnurd , Iran.

Objective: Berberine is an isoquinoline derivative alkaloid with anti-inflammatory activity. In this study, we investigated the protective effects of berberine in prevention of LPS-induced abortion.

Materials And Methods: On the gestation day (GD) 9.5, the pregnant mice were injected with low, medium, and high doses of berberine or with PBS. After 4 h, berberine or PBS-pretreated mice were injected with LPS. On GD 11.5, blood samples and uterine tissues were collected from treated mice and percentage of abortion and serum levels of NO, TNF-α, IL-10, and IL12p70 were measured by macroscopic examination and sandwich ELISA, respectively.

Results: Our findings show that mice injected with berberine were resistant to LPS-induced abortion. We also found that this treatment prevents the reduction of IL-10 and the enhancement of NO, TNF-α, and IL-12p70 in LPS-treated pregnant mice.

Conclusions: Taken together, our results suggest that berberine as an anti-inflammatory agent has protective effects on LPS-induced abortion by modulation of inflammatory/immune responses.
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http://dx.doi.org/10.1080/08923973.2018.1485155DOI Listing
August 2018

Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy.

Clin Exp Reprod Med 2016 Mar 31;43(1):15-25. Epub 2016 Mar 31.

Department of Immunology, School of Medicine, Tarbiat Modares University, Tehran, Iran.

Objective: In the present study, we aimed to evaluate the effects of high doses of dexamethasone (DEX) in early pregnancy on pregnancy outcomes.

Methods: Pregnant BALB/c mice were treated with high-dose DEX in the experimental group or saline in the control group on gestational days (GDs) 0.5 to 4.5. Pregnant mice were sacrificed on GDs 7.5, 13.5, or 18.5 and their peripheral blood, placentas, fetuses, and uterine tissue were collected. Decidual and placenta cell supernatants were examined to evaluate the effect of DEX on the proliferation of mononuclear cells, the quantity of uterine macrophages and uterine natural killer (uNK) cells, and levels of progesterone and 17β-estradiol, as determined by an 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay, immunohistochemistry, and enzyme-linked immunosorbent assay, respectively. We also were measured fetal and placental growth parameters on GD 18.5.

Results: We found that high doses of DEX were associated with an increased abortion rate, enhancement of the immunosuppressive effect of the decidua, alterations in placental growth parameters, decreased progesterone and 17β-estradiol levels, and a reduced frequency of macrophages and uNK cells.

Conclusion: Our data suggest that the high-dose administration of DEX during early pregnancy negatively affected pregnancy outcomes.
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http://dx.doi.org/10.5653/cerm.2016.43.1.15DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838577PMC
March 2016