Publications by authors named "Nazli Pinar Arslan"

5 Publications

  • Page 1 of 1

Effect of Titanium Dioxide and Silver Nanoparticles on Mitochondrial Dynamics in Mouse Testis Tissue.

Biol Trace Elem Res 2021 Jun 8. Epub 2021 Jun 8.

Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.

This study was performed to investigate whether the toxicity of nanoparticles (Ag NPs or TiO NPs) affected mitochondrial dynamics (mitochondrial fusion and fission mechanisms) in testicular cells of mice. Animals were assigned into three groups (ten mice per group): control group (distilled water), TiO NP group (5 mg/kg per dose), and Ag NP group (5 mg/kg per dose). NPs were administered intravenously (via tail vein) to mice with 3-day intervals. To determine the possible toxic effect of NPs on mitochondrial dynamics, the expression levels of mitochondrial fission (Drp1)- and fusion (Mfn1, Mfn2, OPA1)-related genes were analyzed. The results showed that both Ag NPs and TiO NPs entered the testis via the blood-testis barier and accumulated in mouse testis tissue. Experiments showed that administration of Ag NPs neither alters testicular weight and testicular index nor causes significant toxic effect on sperm parameters. RT-PCR analysis demonstrated that Ag NP treatment did not disrupt mitochondrial dynamics in testicular cells. Conversely, administration of TiO NPs (anatase, < 25 nm) decreased the sperm motility and the percentages of sperms with swollen tail. Furthermore, RT-PCR and western blot analyses showed that TiO NPs disrupted mitochondrial dynamics by causing excess mitochondrial fission (excess expression of Drp1 gene and DRP1 protein). This is the first report on the toxicity of nanoparticles on mitochondrial dynamics (fusion and fission mechanisms) in testicular cells.
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http://dx.doi.org/10.1007/s12011-021-02763-6DOI Listing
June 2021

Evaluation of tyrosol and farnesol as inducer in pigment production by Monascus purpureus ATCC16365.

J Basic Microbiol 2020 Aug 25;60(8):669-678. Epub 2020 May 25.

Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.

This study focused on investigating the effect of exogenously applied two quorum sensing molecules (tyrosol and farnesol) on the synthesis of bioactive metabolites (pigments, lactic acid, ethanol, and citric acid) in Monascus purpureus ATCC16365. None of the tested concentrations (62.5, 125, 250, and 500 µl/L) of farnesol affected the synthesis of metabolites as well as cell growth. As with farnesol application, none of the tested concentrations (3.45, 6.9, 13.8, and 27.6 mg/L) of tyrosol caused a significant change in the synthesis of lactic acid and citric acid as well as cell growth. Conversely, all of the tested concentrations of tyrosol increased pigment synthesis but reduced ethanol synthesis, compared with the control. Maximum increases (3.16-, 2.68-, and 2.87-fold increase, respectively) in yellow, orange, and red pigment production were achieved, especially when 6.9-mg/L tyrosol was added to the culture on day 3. On the contrary, 6.9-mg/L tyrosol reduced the content of citrinin by approximately 51.5%. This is the first report on the effect of tyrosol and farnesol on the synthesis of Monascus metabolites. Due to potential properties, such as low price, nonhuman toxicity, promotion of pigment synthesis, and reduction in citrinin synthesis, tyrosol can be used as a novel inducer in the fermentative production of Monascus pigments.
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http://dx.doi.org/10.1002/jobm.202000037DOI Listing
August 2020

Waste frying oil hydrolysis and lipase production by cold-adapted LP2 under non-sterile culture conditions.

Environ Technol 2020 Mar 30:1-9. Epub 2020 Mar 30.

Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.

Non-sterile culture technique is currently used in some microbial processes. However, there is no study on the use of this technique in the production of microbial lipases and hydrolysis of waste frying oils. This study was conducted to hydrolyse waste frying oils and produce lipase under non-sterile culture conditions using locally isolated cold-adapted bacteria. Of 75 bacterial isolates, the psychrotolerant LP2 (Genbank number: KU711080) was determined to have the highest lipase activity. It was found that a combination of restricted nutrient availability, low temperature and high inoculum volume prevented microbial contaminants under non-sterile conditions. The most favourable parameters for lipase production under both sterile and non-sterile conditions were 15°C temperature, pH 8, 30 mL/L inoculum volume, 40 mL/L waste frying oil concentration, 10 mL/L Tween-80 and 72 h incubation time. The maximum lipase activities in sterile and non-sterile media were determined as 93.3 and 96.8 U/L, respectively. The present process designed for enzyme production and waste oil hydrolysis can reduce the cost of cultivation medium as well as energy consumption and workload. The potential of cold-adapted bacteria to produce lipase and hydrolyse waste oils under non-sterile culture conditions was first tested in the current study.
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http://dx.doi.org/10.1080/09593330.2020.1745297DOI Listing
March 2020

Citric acid production from partly deproteinized whey under non-sterile culture conditions using immobilized cells of lactose-positive and cold-adapted Yarrowia lipolytica B9.

J Biotechnol 2016 Aug 24;231:32-39. Epub 2016 May 24.

Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, 25240 Erzurum, Turkey. Electronic address:

The present study was performed to produce citric acid (CA) from partly deproteinized cheese whey (DPCW) under non-sterile culture conditions using immobilized cells of the cold-adapted and lactose-positive yeast Yarrowia lipolytica B9. DPCW was prepared using the temperature treatment of 90°C for 15min. Sodium alginate was used as entrapping agent for cell immobilization. Optimum conditions for the maximum CA production (33.3g/L) in non-sterile DPCW medium were the temperature of 20°C, pH 5.5, additional lactose concentration of 20g/L, sodium alginate concentration of 2%, number of 150 beads/100mL and incubation time of 120h. Similarly, maximum citric acid/isocitric acid (CA/ICA) ratio (6.79) could be reached under these optimal conditions. Additional nitrogen and phosphorus sources decreased CA concentration and CA/ICA ratio. Immobilized cells were reused in three continuous reaction cycles without any loss in the maximum CA concentration. The unique combination of low pH and temperature values as well as cell immobilization procedure could prevent undesired microbial contaminants during CA production. This is the first work on CA production by cold-adapted microorganisms under non-sterile culture conditions. Besides, CA production using a lactose-positive strain of the yeast Y. lipolytica was investigated for the first time in the present study.
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http://dx.doi.org/10.1016/j.jbiotec.2016.05.033DOI Listing
August 2016

Tris-sucrose buffer system: a new specially designed medium for extracellular invertase production by immobilized cells of isolated yeast Cryptococcus laurentii MT-61.

Folia Microbiol (Praha) 2014 Jan 31;59(1):9-16. Epub 2013 May 31.

Department of Biology, Science Faculty, Ataturk University, Erzurum, Turkey.

The aims of the present study were to isolate new yeasts with high extracellular (exo) invertase activity and to investigate the usability of buffer systems as invertase production media by immobilized yeast cells. Among 70 yeast isolates, Cryptococcus laurentii MT-61 had the highest exo-invertase activity. Immobilization of yeast cells was performed using sodium alginate. Higher exo-invertase activity for immobilized cells was achieved in tris-sucrose buffer system (TSBS) compared to sodium acetate buffer system and potassium phosphate buffer system. TSBS was prepared by dissolving 30 g of sucrose in 1 L of tris buffer solution. The optimum pH, temperature, and incubation time for invertase production with immobilized cells were determined as 8.0, 35 °C and 36 h in TSBS, respectively. Under optimized conditions, maximum exo-invertase activity was found to be 28.4 U/mL in sterile and nonsterile TSBS. Immobilized cells could be reused in 14 and 12 successive cycles in sterile and nonsterile TSBS without any loss in the maximum invertase activity, respectively. This is the first report which showed that immobilized microbial cells could be used as a biocatalyst for exo-invertase production in buffer system. As an additional contribution, a new yeast strain with high invertase activity was isolated.
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http://dx.doi.org/10.1007/s12223-013-0258-2DOI Listing
January 2014
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