Publications by authors named "Naoki Noda"

17 Publications

  • Page 1 of 1

Ultra-Fine Bubble Distributions in a Plant Factory Observed by Transmission Electron Microscope with a Freeze-Fracture Replica Technique.

Nanomaterials (Basel) 2018 Mar 8;8(3). Epub 2018 Mar 8.

Minamata Research Center, JNC Co., Minamata, Kumamoto 867-8501, Japan.

Water containing ultra-fine bubbles (UFB) may promote plant growth. But, as UFBs are too small to distinguish from other impurities in a nutrient solution, it is not known if UFBs survive transport from the water source to the rhizosphere. Here we use the freeze-fracture replica method and a transmission electron microscope (TEM) to observe UFBs in the nutrient solutions used in a crop-growing system known as a plant factory. In this factory, TEM images taken from various points in the supply line indicate that the concentration of UFBs in the nutrient solution is conserved, starting from their addition to the nutrient solution in the buffer tank, through the peat-moss layer, all the way to the rhizosphere. Measurements also show that a thin film formed on the surface of UFBs in the nutrient solution, with greater film thickness at the rhizosphere. This film is considered to be made from the accumulation of impurities coming from solute and the peat-moss layer.
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http://dx.doi.org/10.3390/nano8030152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869643PMC
March 2018

Lithocytes are transported along the ciliary surface to build the statolith of ctenophores.

Curr Biol 2014 Oct;24(19):R951-2

Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA; Biology Department, Boston University, Boston, MA 02215, USA. Electronic address:

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http://dx.doi.org/10.1016/j.cub.2014.08.045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265569PMC
October 2014

Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction.

Curr Biol 2011 Oct 13;21(20):1727-31. Epub 2011 Oct 13.

Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA.

Crawling movement in eukaryotic cells requires coordination of leading-edge protrusion with cell body retraction [1-3]. Protrusion is driven by actin polymerization along the leading edge [4]. The mechanism of retraction is less clear; myosin contractility may be involved in some cells [5] but is not essential in others [6-9]. In Ascaris sperm, protrusion and retraction are powered by the major sperm protein (MSP) motility system instead of the conventional actin apparatus [10, 11]. These cells lack motor proteins [12] and so are well suited to explore motor-independent mechanisms of retraction. We reconstituted protrusion and retraction simultaneously in MSP filament meshworks, called fibers, that assemble behind plasma membrane-derived vesicles. Retraction is triggered by depolymerization of complete filaments in the rear of the fiber [13]. The surviving filaments reorganize to maintain their packing density. By packing fewer filaments into a smaller volume, the depolymerizing network shrinks and thereby generates sufficient force to move an attached load. Our work provides direct evidence for motor-independent retraction in the reconstituted MSP motility system of nematode sperm. This mechanism could also apply to actin-based cells and may explain reports of cells that crawl even when their myosin activity is compromised.
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http://dx.doi.org/10.1016/j.cub.2011.08.047DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3210573PMC
October 2011

Rapid and quantitative imaging of excitation polarized fluorescence reveals ordered septin dynamics in live yeast.

Biophys J 2011 Aug;101(4):985-94

Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, USA.

We report an imaging method for fast, sensitive analysis of the orientation of fluorescent molecules by employing a liquid-crystal based universal polarizer in the optical path of a wide-field light microscope. We developed specific acquisition and processing algorithms for measuring the anisotropy and for correcting artifacts caused by fluorescence bleaching, background light, and differential transmission of optical components. We call this approach the Fluorescence LC-PolScope and we used it to analyze the architectural dynamics of septin-green fluorescent protein (septin-GFP) constructs in the neck region of budding yeast. We describe three different states of highly anisotropic septin arrays in which the prevailing orientation of GFP dipoles was either parallel or perpendicular to the mother-bud axis. The transitions between these ordered states were characterized by transient isotropic states. To analyze the patterns of polarized fluorescence, we modeled the alignment of septin-GFP constructs in different stages of septin ring formation. Based on our model, our experimental data are consistent with the formation of paired rather than single filaments and the axis of the α-helical septin terminus linked to a GFP molecule is likely oriented normal to the cell surface. The Fluorescence LC-PolScope combines the molecular specificity of fluorescence tagging with the structural specificity of polarized light analysis.
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http://dx.doi.org/10.1016/j.bpj.2011.07.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175061PMC
August 2011

The absolute configurations of hydroxy fatty acids from the royal jelly of honeybees (Apis mellifera).

Lipids 2011 Mar 17;46(3):263-70. Epub 2010 Nov 17.

Faculty of Pharmaceutical Sciences, Setsunan University, 45-1, Nagaotoge-cho, Hirakata, Osaka, 573-0101, Japan.

9-Hydroxy-2E-decenoic acid (9-HDA) is a precursor of the queen-produced substance, 9-oxo-2E-decenoic acid (9-ODA), which has various important functions and roles for caste maintenance in honeybee colonies (Apis mellifera). 9-HDA in royal jelly is considered to be a metabolite of 9-ODA produced by worker bees, and it is fed back to the queen who then transforms it into 9-ODA. Recently we found that 9-HDA is present in royal jelly as a mixture of optical isomers (R:S, 2:1). The finding leads us to suspect that chiral fatty acids in royal jelly are precursors of semiochemicals. Rather than looking for semiochemicals in the mandibular glands of the queen bee, this study involves the search for precursors of pheromones from large quantities of royal jelly. Seven chiral hydroxy fatty acids, 9,10-dihydroxy-2E-decenoic, 4,10-dihydroxy-2E-decenoic, 4,9-dihydroxy-2E-decenoic, 3-hydroxydecanoic, 3,9-dihydroxydecanoic, 3,11-dihydroxydodecanoic, and 3,10-dihydroxydecanoic acids were isolated. The absolute configurations of these acids were determined using the modified Mosher's method, and it was revealed that, similar to 9-HDA, five acids are present in royal jelly as mixtures of optical isomers.
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http://dx.doi.org/10.1007/s11745-010-3497-xDOI Listing
March 2011

Acylated glycosides of hydroxy fatty acid methyl esters generated from the crude resin glycoside (pharbitin) of seeds of Pharbitis nil by treatment with indium(III) chloride in methanol.

J Nat Prod 2010 Nov 12;73(11):1846-52. Epub 2010 Oct 12.

School of Agriculture, Tokai University, 5435 Minamiaso, Aso, Kumamoto 869-1404, Japan.

Treatment of the crude ether-insoluble resin glycoside (convolvulin) from seeds of Pharbitis nil (Pharbitis Semen), called pharbitin, with indium(III) chloride in methanol provided seven oligoglycosides of hydroxy fatty acid methyl esters partially acylated by 2-methyl-3-hydroxybutyric (nilic) and 2S-methylbutyric acids. Their structures were elucidated on the basis of NMR and MS data and chemical conversions.
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http://dx.doi.org/10.1021/np1004888DOI Listing
November 2010

Assembly of the fluorescent acrosomal matrix and its fate in fertilization in the water strider, Aquarius remigis.

J Cell Physiol 2011 Apr;226(4):999-1006

Graduate Program in Evolution, Ecology, and Organismal Biology, Department of Biology, University of California, Riverside, California 92521, USA.

Animal sperm show remarkable diversity in both morphology and molecular composition. Here we provide the first report of intense intrinsic fluorescence in an animal sperm. The sperm from a semi-aquatic insect, the water strider, Aquarius remigis, contains an intrinsically fluorescent molecule with properties consistent with those of flavin adenine dinucleotide (FAD), which appears first in the acrosomal vesicle of round spermatids and persists in the acrosome throughout spermiogenesis. Fluorescence recovery after photobleaching reveals that the fluorescent molecule exhibits unrestricted mobility in the acrosomal vesicle of round spermatids but is completely immobile in the acrosome of mature sperm. Fluorescence polarization microscopy shows a net alignment of the fluorescent molecules in the acrosome of the mature sperm but not in the acrosomal vesicle of round spermatids. These results suggest that acrosomal molecules are rearranged in the elongating acrosome and FAD is incorporated into the acrosomal matrix during its formation. Further, we followed the fate of the acrosomal matrix in fertilization utilizing the intrinsic fluorescence. The fluorescent acrosomal matrix was observed inside the fertilized egg and remained structurally intact even after gastrulation started. This observation suggests that FAD is not released from the acrosomal matrix during the fertilization process or early development and supports an idea that FAD is involved in the formation of the acrosomal matrix. The intrinsic fluorescence of the A. remigis acrosome will be a useful marker for following spermatogenesis and fertilization.
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http://dx.doi.org/10.1002/jcp.22413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3155764PMC
April 2011

Components of ether-insoluble resin glycoside (convolvulin) from seeds of Quamoclit pennata.

Chem Pharm Bull (Tokyo) 2010 May;58(5):666-72

School of Agriculture, Tokai University, 5435 Minamiaso, Aso, Kumamoto 869-1404, Japan.

Alkaline hydrolysis of the ether-insoluble resin glycoside (convolvulin) fraction of the seeds of Quamoclit pennata BOJER (Convolvulaceae) provided five new glycosidic acids, quamoclinic acids B, C, D, E, and F, along with six organic acids, isobutyric, 2S-methylbutyric, tiglic, 2R,3R-nilic, 7S-hydroxydecanoic, and 7S-hydroxydodecanoic acids. These new compounds were characterized on the basis of spectroscopic data as well as chemical evidence. Quamoclinic acids E and F are the first examples of heptaglycosides of glycosidic acid.
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http://dx.doi.org/10.1248/cpb.58.666DOI Listing
May 2010

Novel cycloartane-type triterpenoid from the fruits of Nandina domestica.

J Nat Med 2010 Apr 30;64(2):216-8. Epub 2010 Jan 30.

Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka, Japan.

A novel cycloartane-type triterpenoid was isolated from the fruits of Nandina domestica (Berberidaceae). The structure was characterized as 24-methylene-3-oxocycloartane 13-carboxylic acid on the basis of NMR spectroscopic data.
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http://dx.doi.org/10.1007/s11418-010-0389-6DOI Listing
April 2010

The resin glycosides from the sweet potato (Ipomoea batatas L. LAM.).

Chem Pharm Bull (Tokyo) 2008 Nov;56(11):1607-10

Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.

Four new and two known ether-soluble resin glycosides were isolated from popular sweet potato (the roots of Ipomoea batatas L. LAM., Kokei 14 go, Convolvulaceae) in Japan. Unlike ester-type dimers, batatins I and II, obtained from other sweet potato (Ipomoea batabas var. batatas), the glycosides were tetra or pentasaccharide monomers in which the sugar moieties are partially acylated by organic acids and combine with the aglycone, jalapinolic acid, to form a macrocyclic ester.
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http://dx.doi.org/10.1248/cpb.56.1607DOI Listing
November 2008

A new microscope optics for laser dark-field illumination applied to high precision two dimensional measurement of specimen displacement.

Rev Sci Instrum 2008 Feb;79(2 Pt 1):023704

Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan.

With conventional light microscopy, precision in the measurement of the displacement of a specimen depends on the signal-to-noise ratio when we measure the light intensity of magnified images. This implies that, for the improvement of precision, getting brighter images and reducing background light noise are both inevitably required. For this purpose, we developed a new optics for laser dark-field illumination. For the microscopy, we used a laser beam and a pair of axicons (conical lenses) to get an optimal condition for dark-field observations. The optics was applied to measuring two dimensional microbead displacements with subnanometer precision. The bandwidth of our detection system overall was 10 kHz. Over most of this bandwidth, the observed noise level was as small as 0.1 nm/radicalHz.
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http://dx.doi.org/10.1063/1.2839914DOI Listing
February 2008

Compositions of royal jelly II. Organic acid glycosides and sterols of the royal jelly of honeybees (Apis mellifera).

Chem Pharm Bull (Tokyo) 2007 Oct;55(10):1528-31

Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan.

Two organic acid glycosides (1, 2) and 16 sterols were isolated from the royal jelly of honeybees (Apis mellifera). The former two were monoglucosides of 10-hydroxy-2E-decenoic and 10-hydroxydecanoic acids. They are the first examples of glycosides isolated from royal jelly. The latter 16 were sterols mainly composed of 28 or 29 carbons. Among them, four compounds were new isofucosterol derivatives, and their structures were characterized as (24Z)-stigmasta-5,24(28)-dien-3beta-ol-7-one (3), (24Z)-stigmasta-5,24(28)-diene-3beta,7beta-diol (4), (24Z)-stigmasta-5,24(28)-diene-3beta,7alpha-diol (5), and (24Z)-stigmast-24(28)-ene-3beta,5alpha,6beta-triol (6) on the basis of various NMR spectroscopic data.
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http://dx.doi.org/10.1248/cpb.55.1528DOI Listing
October 2007

Cantharimide dimers from the Chinese blister beetle, Mylabris phalerate PALLAS.

Chem Pharm Bull (Tokyo) 2007 Jan;55(1):92-4

Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka 573-0101, Japan.

Five cantharidin-related compounds were isolated from the Chinese blister beetle, Mylabris phalerate PALLAS (Meloidae). Their structures were determined based on spectroscopic and chemical evidence. Three of them were identified as cantharimide dimers, which consist of two units of cantharimide combined with a tri-, tetra-, or penta-methylene group.
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http://dx.doi.org/10.1248/cpb.55.92DOI Listing
January 2007

Two isoindoline alkaloids from the crude drug, the ant lion (the larvae of Myrmeleontidae species).

J Nat Med 2006 Jul 24;60(3):261-263. Epub 2006 May 24.

Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka, 573-0101, Japan.

Two compounds having an isoindoline skeleton were isolated from the ant lion (the larvae of Myrmeleontidae species). They were characterized as 4-hydroxyisoindolin-1-one and 2-(2-hydroxyethyl)-4-hydroxyisoindolin-1-one on the basis of spectroscopic data.
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http://dx.doi.org/10.1007/s11418-006-0049-zDOI Listing
July 2006

Isolation and characterization of some hydroxy fatty and phosphoric acid esters of 10-hydroxy-2-decenoic acid from the royal jelly of honeybees (Apis mellifera).

Lipids 2005 Aug;40(8):833-8

Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.

The present work characterizes novel FA in the royal jelly of honeybees (Apis mellifera). TLC analysis showed that the chloroform/methanol extract obtained from royal jelly consists mainly of FA. The FABMS spectrum of this extract gave several ion peaks due to compounds with higher M.W. than those of the FA so far reported. The methanol extract was found to contain unknown phospholipids. By means of reversed-phase HPLC with various solvent systems, 13 compounds were obtained in pure state. Their structures including absolute configurations were determined by chemical, NMR, and MS spectral analysis. Six compounds were identified as novel mono- or di-esters of 10-hydroxy-2E-decenoic acid in which the hydroxyl group was esterified by another FA unit, and one was hydroxy-2E-decenoic acid 10-phosphate. In addition, we demonstrated that 9-hydroxy-2E-decenoic acid exists as a mixture of optical isomers.
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http://dx.doi.org/10.1007/s11745-005-1445-6DOI Listing
August 2005

Fluctuation of actin sliding over myosin thick filaments .

Biophysics (Nagoya-shi) 2005 16;1:45-53. Epub 2005 Jun 16.

Department of Biology, Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan.

It is customarily thought that myosin motors act as independent force-generators in both isotonic unloaded shortening as well as isometric contraction of muscle. We tested this assumption regarding unloaded shortening, by analyzing the fluctuation of the actin sliding movement over long native thick filaments from molluscan smooth muscle . This analysis is based on the prediction that the effective diffusion coefficient of actin, a measure of the fluctuation, is proportional to the inverse of the number of myosin motors generating the sliding movement of an actin filament, hence proportional to the inverse of the actin length, when the actions of the motors are stochastic and statistically independent. Contrary to this prediction, we found the effective diffusion coefficient to be virtually independent of, and thus not proportional to, the inverse of the actin length. This result shows that the myosin motors are not independent force-generators when generating the continuous sliding movement of actin and that the sliding motion is a macroscopic manifestation of the cooperative actions of the microscopic ensemble motors.
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http://dx.doi.org/10.2142/biophysics.1.45DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036633PMC
June 2005

Three novel cantharidin-related compounds from the Chinese blister beetle, Mylabris phalerata Pall.

Chem Pharm Bull (Tokyo) 2004 Jul;52(7):807-9

Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.

Three novel cantharidin analogues were isolated from the Chinese blister beetle, Mylabris phalerata PALL. (Meloidae), which has been used in traditional Chinese medicine for the treatment of cancer. Their structures were determined on the basis of heteronuclear multiple-bond connectivity and nuclear Overhauser effect spectroscopy experiments, and chemical data confirmed them to be so-called cantharimides, in which the anhydride oxygen atoms are replaced by the basic amino acid L-lysine, L-ornithine, and L-arginine moieties.
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http://dx.doi.org/10.1248/cpb.52.807DOI Listing
July 2004
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