Publications by authors named "Nanis G Allam"

5 Publications

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A new trend in the medication of hepatocyte cytoxicity in mice: protective role of probiotic bacteria.

Environ Sci Pollut Res Int 2021 Jan 26;28(2):1555-1564. Epub 2020 Aug 26.

Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Liver toxicity is affected by several factors, including certain medications, fumes emission from factories, materials used in industries, and exposure to chemicals such as carbon tetrachloride (CCl). Some preselected probiotic bacteria strains have been widely employed in different medical researches due to their antioxidant, anticancer, antimicrobial, anti-inflammatory characters, and hepatoprotective factor. The present study was aimed to evaluate the protective role of probiotic bacteria (Lactobacillus plantarum DSMZ 20174) and their ameliorative effects against CCl-induced hepatotoxicity in mice. The cell cycle of hepatocytes and the expression of transforming growth factor-β (TGF-β) were assessed by flow cytometry as indicators for apoptosis. The antioxidant activity of probiotic bacteria was estimated by measuring lipid peroxidation (LPO) and scavenging 2,2-diphenyl-1-picryl-hydrazyl (DPPH). The results showed that the treatment of CCl-administered mice by supernatant from Lactobacillus plantarum DSMZ 20174 induced an amelioration in CCl-induced increases in serum activity of the liver enzymes and decreases in LPO and DPPH. After treatment with probiotics, the liver histopathological studies showed abundant infiltration and accumulation of mononuclear cells and fibroblast, indicating a positive effect ameliorating the damage previously induced by CCl. In sum, the results of the present work indicate the protective effects of Lactobacillus plantarum against hepatotoxicity through antioxidant effects.
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http://dx.doi.org/10.1007/s11356-020-10420-zDOI Listing
January 2021

The role of silver nanoparticles biosynthesized by and cyanobacteria for malachite green removal from wastewater.

Environ Technol 2020 May 23:1-15. Epub 2020 May 23.

Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.

In this study, biosynthesis of silver nanoparticles (AgNPs) using two species of cyanobacteria, (Kütz) and (Gomont) was investigated and evaluated for dye removing capacity. The formation of AgNPs was detected by the change in colour using UV-Vis spectroscopy and further characterised by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy FTIR). The obtained AgNPs were spherical to oval with average particle size of 17.9 and 26.4 nm for and respectively. These AgNPs were applied as bio-sorbent for the removal of malachite green (MG) dye released into wastewater. Within the tested initial concentration range of MG, the reaction exhibited first order kinetics model as monitored via UV spectroscopy. As the dye concentration decreased, the removal efficiency increased to reach 93% for and 82% for AgNPs. The results also indicated that increased AgNPs concentration enhanced the MG removal with an efficiency up to 88% and 81% for and AgNPs, respectively. The smaller particle diameter and larger specific surface area of AgNPs enabled boosted catalytic activity for dye removal than those of . After the treatment with AgNPs, the resultant dye- AgNPs-free effluent proved non-toxic to L (Giza 171) seedlings, implying their safety for cultivation practices.
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http://dx.doi.org/10.1080/09593330.2020.1766576DOI Listing
May 2020

Biosynthesis of silver nanoparticles by cell-free extracts from some bacteria species for dye removal from wastewater.

Biotechnol Lett 2019 Mar 28;41(3):379-389. Epub 2019 Jan 28.

Chemistry Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.

Objectives: To investigate the biosynthesis of silver nanoparticles (AgNPs) using extracts of some bacterial isolates Bacillus pumilus, Bacillus paralicheniformis and Sphingomonas paucimobilis. The formation of AgNPs was detected by the change in color into yellow and confirmed by the UV-Vis spectroscopy. The nanoparticles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR).

Results: The obtained AgNPs were spherical to oval in shape with particle size ranged from 4 to 20 nm and surface area 118 m/g. The AgNPs have been used as nanocatalyst for the removal of malachite green dye (MG) from aqueous solution. The dye was chosen as a model dye released in wastewater. The AgNPs showed excellent nanocatalyst for the removal of MG. The dye removal process was observed by the continuous decrease in dye absorbance at 617 nm until it vanished over 160 min. The removal kinetics followed closely the pseudo-first-order kinetic model.

Conclusion: The B. paralicheniformis strain KJ-16 was the most effective isolated bacteria to give extract for biosynthesis of AgNPs and dye removal. This method may be considered easy and eco-friendly, and could be applicable for large-scale decontamination of wastewater from harmful dyes.
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http://dx.doi.org/10.1007/s10529-019-02652-yDOI Listing
March 2019

Protective Efficacy of  Against Acute Cadmium Toxicity in Mice.

Iran J Pharm Res 2018 ;17(2):695-707

Microbiology unit, Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.

Cadmium (Cd) is a highly toxic heavy metal, wide occupational and an environmental pollutant, affecting human health. Probiotics especially lactic acid bacteria (LAB) have the capacity to bind, remove and to decrease tissue cadmium levels. The objective was to evaluate the potency of Cd binding capacity, antioxidative properties of probiotic bacteria against cadmium and its probable detoxification effect against Cd-induced toxicity in mice. To asses this objective, resistance against cadmium and antioxidative properties (via DPPH radical scavenging and inhibition of lipid peroxidation) were estimated for thirteen probiotic bacteria. was selected among investigated bacteria as it had the highest MIC against cadmium and remarkable antioxidant activities for treatment of Cd toxicity in Swiss albino mice by preventive and therapeutic protocols. Blood cadmium levels, reduced glutathione (GSH), malondialdehyde (MDA) and histopathological changes in the liver of mice were estimated at 6, 24 and 48 h post to acute Cd exposure (oral dose with 50 mg/kg body weight). On exposure to Cd a significant increase in blood Cd, MDA and reducing in GSH levels were observed. offered a significant protective effect against Cd toxicity by decreasing the cadmium levels in blood and attenuation alterations in the levels of GSH and MDA and improved hepatic histopathological changes caused by Cd toxicity. These results indicated the protective action of against acute cadmium toxicity as well as their beneficial health effects and suggested its use as a safe and efficacious nutritional dietary supplement to reduce cadmium toxicity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5985187PMC
January 2018

Screening of microbial contamination and antimicrobial activity of sea cucumber Holothuria polii.

Toxicol Ind Health 2013 Nov 31;29(10):944-54. Epub 2012 May 31.

1Invertebrate Unit, Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.

Microbiological studies were carried out on microbial contamination and antimicrobial activity of sea cucumber Holothuria polii collected from Mediterranean Sea at Abu-kir shore of Alexandria, Egypt. The obtained results revealed the presence of isolates of five human Gram-negative pathogenic bacteria, representing five genera were identified to species level, including, Esherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella sp. and Shigella sp. In addition, an yeast Candida albicans was isolated. The pathogenic bacteria were identified using API 20E strip system (BioMereux). All collected H. polii specimens were healthy with no external signs of infection. Histopathological study of the tegument, intestine and gonads showed no abnormal changes. The antimicrobial activity of two tegumental ethanol extracts (A and B, differ in the method of dehydration) were tested against wide range of pathogenic bacteria and fungi, including intestinal, skin and nosocomial pathogens and one plant fungal pathogen. The results revealed a remarkable antifungal activity of the extract B at 2.5 mg/ml MIC90, especially on Aspergillus niger, Scloretium sp, C. albicans, Aspergillus flavus and Malassezia furfur, and limited antibacterial activity against Gram-negative bacteria (Salmonella choleraesuis ATCC 14028 and Aeromonas hydrophila). The domain of bacterial and limited fungal contamination confirms the results that showed strong antifungal activity of investigated extract.
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http://dx.doi.org/10.1177/0748233712448116DOI Listing
November 2013