Publications by authors named "Nana Zhang"

107 Publications

Characteristic thermal denaturation profile of myosin in the longitudinal retractor muscle of sea cucumber (Stichoupus japonicas).

Food Chem 2021 Mar 18;357:129606. Epub 2021 Mar 18.

National Engineering Research Center of Seafood, Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address:

This study elucidated thermal denaturation profile of myosin in sea cucumber longitudinal muscle. Sea cucumber myosin structure was different from fish at its head/tail junction which could not be cleaved by EDTA. However, sea cucumber myosin in salt-dissolved form could be cleaved into heavy meromyosin (HMM) and light meromyosin (LMM) segments. Although sea cucumbers lived in cold water, its myosin stability was comparable to tropical tilapia, more stable than rainbow trout (a cold water fish). Upon heating, the sea cucumber myosin lost its salt-solubility rapidly, even before losing its ATPase activity. The quick loss of salt-solubility suggested a quick denaturation at light meromyosin region as revealed by chymotryptic digestion. These results suggested that sea cucumber myosin is consisted of very stable head region and unstable tail region, which is important for choosing proper heating conditions for sea cucumber processing.
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http://dx.doi.org/10.1016/j.foodchem.2021.129606DOI Listing
March 2021

Donor plasma mitochondrial DNA is associated with antibody-mediated rejection in renal allograft recipients.

Aging (Albany NY) 2021 03 10;13(6):8440-8453. Epub 2021 Mar 10.

Organ Transplantation Research Institution, Division of Kidney Transplantation, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.

We previously showed that donor plasma mitochondrial DNA (dmtDNA) levels were correlated with renal allograft function. The aim of the current study was to determine whether dmtDNA levels are associated with the occurrence of antibody-mediated rejection (ABMR). This is a retrospective open cohort study comprised of 167 donors and 323 recipients enrolled from January 2015 to December 2017. We quantified the mtDNA level present in donor plasma using quantitative real-time polymerase chain reaction. The average plasma dmtDNA level in the acute rejection (AR) group was higher than that of the control group (0.156 versus 0.075, p<0.001). Multivariate logistic regression analysis showed that dmtDNA levels were also significantly associated with AR (OR=1.588, 95% CI 1.337-4.561, p<0.001). When the dmtDNA level was >0.156, the probability of AR was 62.9%. The plasma dmtDNA level in the ABMR group was significantly higher than that of the T cell-mediated rejection group (0.185 versus 0.099, p=0.032). The area under the receiver operating characteristic curve of dmtDNA for prediction of ABMR was as high as 0.910 (95% CI 0.843-0.977). We demonstrated that plasma dmtDNA was an independent risk factor for ABMR, which is valuable in organ evaluation. dmtDNA level is a possible first predictive marker for ABMR.
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http://dx.doi.org/10.18632/aging.202654DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034952PMC
March 2021

Oncolytic adenoviral H101 synergizes with radiation in cervical cancer cells.

Curr Cancer Drug Targets 2021 Mar 7. Epub 2021 Mar 7.

Institute of Molecular Radiobiology of Cancer, Department of Radiation Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xian. China.

Background: A major challenge in cervical cancer radiotherapy is to tailor the radiation doses efficiently to both eliminate malignant cells and to reduce the side effects to normal tissue. Oncolytic adenoviral drug H101 is recently tested and approved for topical adjuvant treatment of several malignancies.

Objective: This study is to evaluate the potential neoadjuvant radiotherapy benefits of H101 by testing the inhibitory function of H101 combined with radiation in different cervical cancer cells.

Methods: Human cervical cancer cells C33a, SiHa, CaSki, and Hela were treated with varying concentrations of H101 alone or combined with radiation (2Gy or 4Gy). Cell viability and apoptosis were measured at indicated time intervals. HPV16 E6 and cellular p53 mRNA expression alteration were measured by qRT-PCR. RNA scope in-situ detect HPV E6 status. P53 protein alteration are detected by Western blot.

Results: Cell viability and apoptosis show the combination of a high dose of H101 (MOI=1000, 10000) with radiation yielded a synergistic anti-cancer effect in all tested cervical cancer cell lines (P<0.05), with the greatest effect achieved in HPV negative C33a cells (P<0.05). Low HPV16 viral load SiHa cell was more sensitive to combination therapy than high HPV16 viral load CaSki cell (P<0.05). The combined treatment could reduce HPV16 E6 expression and increase cellular P53 level compared to radiation alone in SiHa and CaSki (P<0.05).

Conclusions: Oncolytic adenoviral H101 effectively enhances the antitumor efficacy of radiation in cervical cancer cells and may serve as a novel combination therapy for cervical cancer.
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http://dx.doi.org/10.2174/1568009621666210308103541DOI Listing
March 2021

CTSB Knockdown Inhibits Proliferation and Tumorigenesis in HL-60 Cells.

Int J Med Sci 2021 29;18(6):1484-1491. Epub 2021 Jan 29.

Department of Pathology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou510000, P. R. China.

Cathepsin B (CTSB) was well documented in solid tumors, up-regulated of CTSB expression is linked with progression of tumors. However, the study of CTSB in adult leukemia has not been reported. Total RNA was isolated from PBMC (peripheral blood mononuclear cell) of AML patients and healthy donors. qRT-PCR was performed to detect the expression of CTSB. The association of CTSB expression with the patients' overall survival (OS) and disease-free survival (DFS) were analyzed. Stable HL-60 CTSB-shRNA cell lines were established by retrovirus infection and puromycin selection. Cell proliferation was detected by CCK-8 analysis. Tumorigenesis ability was analyzed by soft agar and xenograft nude mice model. Western blot was performed to detect the expression of CTSB and the proteins of cell signaling pathway. The mRNA expression level of CTSB was up-regulated in AML patients compared to healthy control (<0.001), and CTSB expression was significantly higher in M1, M2, M4 and M5 AML samples than healthy control. The CTSB expression in AML was associated with WBC count (=0.037). Patients with high CTSB expression had a relatively poor OS (=0.007) and a shorter DFS (=0.018). Moreover, the expression level of CTSB may act as an independent prognostic factor for both OS (=0.011) and DFS (=0.004). Knockdown CTSB expression in HL-60 cells could inhibit the cells' proliferation and tumorigeneses and . Further study showed knockdown CTSB expression in HL-60 cells could inactive the AKT signaling pathway. CTSB mRNA was upregulated in AML patients. CTSB overexpression was correlated with poor prognosis and may serve as an independent prognostic factor for both OS and DFS in AML patients. Knockdown CTSB expression in HL-60 cells could inhibit the cells' proliferation and tumorigenesis. The underlying mechanism may be the inhibition of the AKT signaling pathway.
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http://dx.doi.org/10.7150/ijms.54206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893552PMC
January 2021

Fecal microbiota transplantation for rheumatoid arthritis: A case report.

Clin Case Rep 2021 Feb 23;9(2):906-909. Epub 2020 Dec 23.

Department of Gastroenterology and Hepatology The First Medical Center Chinese PLA General Hospital Beijing China.

No previous case of using fecal microbiota transplantation (FMT) to treat rheumatoid arthritis (RA) has been reported. We report a case of a patient with refractory RA successfully treated with FMT indicating that FMT may have a good therapeutic effect on RA.
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http://dx.doi.org/10.1002/ccr3.3677DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869316PMC
February 2021

Metabolomics-based multidimensional network biomarkers for diabetic retinopathy identification in patients with type 2 diabetes mellitus.

BMJ Open Diabetes Res Care 2021 Feb;9(1)

Eye Hospital and School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China

Introduction: Despite advances in diabetic retinopathy (DR) medications, early identification is vitally important for DR administration and remains a major challenge. This study aims to develop a novel system of multidimensional network biomarkers (MDNBs) based on a widely targeted metabolomics approach to detect DR among patients with type 2 diabetes mellitus (T2DM) efficiently.

Research Design And Methods: In this propensity score matching-based case-control study, we used ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry system for serum metabolites assessment of 69 pairs of patients with T2DM with DR (cases) and without DR (controls). Comprehensive analysis, including principal component analysis, orthogonal partial least squares discriminant analysis, generalized linear regression models and a 1000-times permutation test on metabolomics characteristics were conducted to detect candidate MDNBs depending on the discovery set. Receiver operating characteristic analysis was applied for the validation of capability and feasibility of MDNBs based on a separate validation set.

Results: We detected 613 features (318 in positive and 295 in negative ESI modes) in which 63 metabolites were highly relevant to the presence of DR. A panel of MDNBs containing linoleic acid, nicotinuric acid, ornithine and phenylacetylglutamine was determined based on the discovery set. Depending on the separate validation set, the area under the curve (95% CI), sensitivity and specificity of this MDNBs system were 0.92 (0.84 to 1.0), 96% and 78%, respectively.

Conclusions: This study demonstrates that metabolomics-based MDNBs are associated with the presence of DR and capable of distinguishing DR from T2DM efficiently. Our data also provide new insights into the mechanisms of DR and the potential value for new treatment targets development. Additional studies are needed to confirm our findings.
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http://dx.doi.org/10.1136/bmjdrc-2020-001443DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888330PMC
February 2021

Fenton reaction-mediated dual-attenuation of signal for ultrasensitive amperometric immunoassay.

Biosens Bioelectron 2021 Apr 21;178:113009. Epub 2021 Jan 21.

Department of Chemistry, Capital Normal University, Beijing, 100048, China. Electronic address:

In order to alter the complexion of immunoprobe with large impedance as negative factor in sensitivity of amperometric immunosensor, a strategy of Fenton reaction-mediated dual-attenuation of signal was proposed. Herein, metal-polydopamine-Fe composite with the ability of Fenton reaction was initially prepared as immunoprobe for an ultrasensitive immunoassay. The polymerization of dopamine occurred on the surface of ZIF-67 to gain the metal-polydopamine shell, which possessed rich functional groups, negative charge and high specific surface. Then the prepared functional shell was further used to absorb Fe and immobilize labeling antibody as immunoprobe, which was used to construct a sandwich type immunosensor. With addition of HO and aniline, Fenton reaction was triggered to produce hydroxyl radicals, which can not only decrease the current value by degrading methylene blue molecules, but also further initiate aniline to polymerize into non-conductive polyaniline for successive abatement of signal intensity. Therefore, the dual-attenuation of signal model rendered the immunoprobe into a favorable factor and synchronously enhance sensitivity. Expectedly, the detection performance with a linear range from 1.0 × 10-100 ng mL and ultralow detection limit of 9.07 × 10 ng mL toward neuron-specific enolase was obtained under optimal conditions. This work offered a novel tactic for enhancing sensitivity of immunosensor through the preparation of functional immunoprobe and its rational utilization as signal enhancer.
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http://dx.doi.org/10.1016/j.bios.2021.113009DOI Listing
April 2021

Low- versus High-Chloride Content Intravenous Solutions for Perioperative Patients: A Systematic Review and Meta-Analysis.

Biomed Res Int 2021 2;2021:3571397. Epub 2021 Jan 2.

ICU, DongE Hospital Affiliated to Shandong First Medical University, Liaocheng, China.

Background: Studies have shown complications of normal saline infusion because of its high-chloride content. Therefore, in the present study, we aimed to explore whether the use of low- versus high-chloride solutions benefited the unselected and specifically perioperative patients and was associated with different outcomes.

Methods: Studies on the use of low- versus high-chloride content intravenous solutions for perioperative patients, published up to July 15, 2019, were systematically reviewed, and primary and secondary outcomes were quantitatively summarized.

Results: A total of 14 eligible randomized controlled trials with 943 perioperative patients were included. Five studies reported all-cause mortality, and eight studies provided detailed data on renal replacement therapy (RRT). The pooled result suggested no statistically significant difference in the effect of low- versus high-chloride solutions on all-cause mortality (risk ratio (RR) = 1.39; 95%confidence interval (CI) = 0.23-8.26) and RRT (RR = 1.05; 95%CI = 0.63-1.76). The pooled results on acute kidney injury (AKI) and the use of allogenic blood transfusion ( > 0.05) were similar.

Conclusion: Among specific perioperative patients, the use of low- versus high-chloride content intravenous solutions did not reduce the all-cause mortality, risk of severe AKI, or rate of RRT use. Further large randomized clinical trials are needed to confirm or refute this finding.
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http://dx.doi.org/10.1155/2021/3571397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7801087PMC
January 2021

Estimating freshness of ice storage rainbow trout using bioelectrical impedance analysis.

Food Sci Nutr 2021 Jan 14;9(1):154-163. Epub 2020 Nov 14.

National Engineering Research Center of Seafood Collaborative Innovation Center of Provincial and Ministerial Co-construction for Seafood Deep Processing Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing School of Food Science and Technology Dalian Polytechnic University Dalian 116034 China.

This study aimed to evaluate the freshness of ice stored rainbow trout by bioelectrical impedance measurements. Rigor mortis, ATP-related components, K-value, and hardness of rainbow trout muscle during storage were monitored along with impedance. The results showed that the progress of rigor mortis was accompanied by an increase in impedance. Impedance kept decreasing even in rigor state, and during the gradual resolution of rigor mortis with impedance change upon storage of fish was biphasic ( = -0.944,  < .01). Thus, when impedance decreased close to the lowest value, K-value was only about 61.57 ± 0.52%, but still exhibited a high pertinence ( = -0.959,  < .01). A gradual decrease of the hardness of fish muscle upon storage of fish showed a close correlation ( = 0.981,  < .01) with impedance decrease. These results suggested that the impedance measurement has a great potential for predicting the freshness of the rainbow trout during ice storage.
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http://dx.doi.org/10.1002/fsn3.1974DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802552PMC
January 2021

Effect of Cover Plate on the Ballistic Performance of Ceramic Armor.

Materials (Basel) 2020 Dec 22;14(1). Epub 2020 Dec 22.

School of Astronautics, Harbin Institute of Technology, Harbin 150080, China.

The interface defeat and dwell can effectively improve the ballistic performance of ceramic armors under high velocity impact of long rod projectiles. Confinement conditions along both axial and radial directions of ceramic armors can affect these behaviors. With the aim of giving an insight into the effect of cover plate thickness and connection mode of cover plates with confining tubes on these behaviors, numerical simulations were performed in which the confined silicon carbide (SiC) targets with cover plates were impacted by tungsten rods. The pressure on the surfaces of SiC targets with fixed cover plates are compared to that with free cover plates, showing that the plates fixed with the confining tubes can produce higher pressure by way of wedging. With the increase in cover plate thickness, the dwell duration of the tungsten rods on the ceramic interface gradually grows. In addition, the upper and lower limits of transition impact velocities for the SiC targets with cover plates in different connection modes (i.e., free or fixed) were obtained and analyzed. The results show that the increase rate of the transition velocity region for the cover plate with the fixed-mode is relatively stable and lower than with the free-mode. On this basis, the fixed cover plate contributes higher ballistic performances to the SiC target than the free cover plate. It is also noteworthy that the size of transition velocity region does not enlarge linearly with the increase in cover plate thickness due to the slow growth of the upper limit. Accordingly, thickness thresholds exist, which are 5 mm and 6 mm for the fixed and free cover plates, respectively. Considering the ballistic performance and economy, the cover plate with the thickness ranging from 3 mm to 5 mm, i.e., 1.5~2.5 times of the tungsten rod diameter, is ideal for the structural dimensions in this paper.
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http://dx.doi.org/10.3390/ma14010001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792607PMC
December 2020

Galectin-9 regulates follicular helper T cells to inhibit humoral autoimmunity-induced pulmonary fibrosis.

Biochem Biophys Res Commun 2021 01 11;534:99-106. Epub 2020 Dec 11.

Department of Respiratory, Honghui Hospital, Xi'an Jiaotong University, PR China. Electronic address:

Interstitial pneumonia with autoimmune features (IPAF) is an unexplained disease state characterized by autoimmunity and pulmonary fibrosis. Exploring the pathogenesis of IPAF is helpful for the treatment of interstitial pneumonia and idiopathic pulmonary fibrosis. In this study, we observed that the lung Galectin-9 (Gal-9) of IPAF patients was significantly reduced, which was significantly related to lung dysfunction and abnormal humoral immunity. Moreover, an overreactive germinal center (GC) reaction in the lung lymph nodes (LNs) of Gal-9-deficient mice was found to be related to abnormally active follicular helper T cells (Tfh) cells. The lack of Gal-9 ligand in Tfh cells can lead to excessive transcriptional programming and differentiation and help GC B cells. Gal-9 deficiency caused an abnormal humoral immune response in mice, leading to excessive deposition of nonspecific autoantibodies in mice and chronic lung fibrosis. Our research reveals the important regulatory role of gal-9 in Tfh cells and a possible target for the treatment of IPAF.
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http://dx.doi.org/10.1016/j.bbrc.2020.11.097DOI Listing
January 2021

ppGalNAc-T4-catalyzed O-Glycosylation of TGF-β type Ⅱ receptor regulates breast cancer cells metastasis potential.

J Biol Chem 2020 Dec 3;296:100119. Epub 2020 Dec 3.

School of Life Science & Pharmacy, Dalian University of Technology, Panjin, China. Electronic address:

GalNAc-type O-glycosylation, initially catalyzed by polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts), is one of the most abundant and complex posttranslational modifications of proteins. Emerging evidence has proven that aberrant ppGalNAc-Ts are involved in malignant tumor transformation. However, the exact molecular functions of ppGalNAc-Ts are still unclear. Here, the role of one isoform, ppGalNAc-T4, in breast cancer cell lines was investigated. The expression of ppGalNAc-T4 was found to be negatively associated with migration of breast cancer cells. Loss-of-function studies revealed that ppGalNAc-T4 attenuated the migration and invasion of breast cancer cells by inhibiting the epithelial-mesenchymal transition (EMT) process. Correspondingly, transforming growth factor beta (TGF-β) signaling, which is the upstream pathway of EMT, was impaired by ppGalNAc-T4 expression. ppGalNAc-T4 knockout decreased O-GalNAc modification of TGF-β type Ⅰ and Ⅱ receptor (TβR Ⅰ and Ⅱ) and led to the elevation of TGF-β receptor dimerization and activity. Importantly, a peptide from TβR Ⅱ was identified as a naked peptide substrate of ppGalNAc-T4 with a higher affinity than ppGalNAc-T2. Further, Ser31, corresponding to the extracellular domain of TβR Ⅱ, was identified as the O-GalNAcylation site upon in vitro glycosylation by ppGalNAc-T4. The O-GalNAc-deficient S31 A mutation enhanced TGF-β signaling activity and EMT in breast cancer cells. Together, these results identified a novel mechanism of ppGalNAc-T4-catalyzed TGF-β receptors O-GalNAcylation that suppresses breast cancer cell migration and invasion via the EMT process. Targeting ppGalNAc-T4 may be a potential therapeutic strategy for breast cancer treatment.
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http://dx.doi.org/10.1074/jbc.RA120.016345DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7948473PMC
December 2020

Proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins reveal an O-GlcNAc-regulated genotoxic stress response.

Nat Commun 2020 11 19;11(1):5898. Epub 2020 Nov 19.

School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, China.

O-GlcNAc modification plays critical roles in regulating the stress response program and cellular homeostasis. However, systematic and multi-omics studies on the O-GlcNAc regulated mechanism have been limited. Here, comprehensive data are obtained by a chemical reporter-based method to survey O-GlcNAc function in human breast cancer cells stimulated with the genotoxic agent adriamycin. We identify 875 genotoxic stress-induced O-GlcNAc chromatin-associated proteins (OCPs), including 88 O-GlcNAc chromatin-associated transcription factors and cofactors (OCTFs), subsequently map their genomic loci, and construct a comprehensive transcriptional reprogramming network. Notably, genotoxicity-induced O-GlcNAc enhances the genome-wide interactions of OCPs with chromatin. The dynamic binding switch of hundreds of OCPs from enhancers to promoters is identified as a crucial feature in the specific transcriptional activation of genes involved in the adaptation of cancer cells to genotoxic stress. The OCTF nuclear factor erythroid 2-related factor-1 (NRF1) is found to be a key response regulator in O-GlcNAc-modulated cellular homeostasis. These results provide a valuable clue suggesting that OCPs act as stress sensors by regulating the expression of various genes to protect cancer cells from genotoxic stress.
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http://dx.doi.org/10.1038/s41467-020-19579-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678849PMC
November 2020

Brain protective effect and hemodynamics of dexmedetomidine hydrochloride in patients with intracranial aneurysm.

Saudi J Biol Sci 2020 Jul 10;27(7):1850-1855. Epub 2020 Apr 10.

Department of Anesthesiology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, China.

The purpose of the study was to investigate the effect of dexmedetomidine hydrochloride (Dex) on the recovery of cognitive function, hemodynamics, and postoperative analgesia in patients undergoing intracranial aneurysm craniotomy.

Methods: general anesthesia was performed on patients undergoing intracranial aneurysm craniotomy in neurosurgery. Patients were randomly divided into three groups: Dex 1 group (Dex dose: 1 μg/kg), Dex 2 group (Dex dose: 0.5 μg/kg), and blank control group (normal saline). The changes of heart rate, arterial pressure, intraoperative brain function index, and postoperative pain score were recorded and compared.

Results: in Dex 1 group and Dex 2 group, the heart rate of T1 and T2 phase was significantly lower than that of T3-T7 phases (P < 0.05); compared with the control group, the heart rate of Dex 1 group and Dex 2 group was significantly lower (P < 0.05). The average arterial pressure of the control group and Dex groups was significantly different (P < 0.05). Compared with the control group, there were significant differences between Dex 1 group and Dex 2 group: S100 β protein in T7-T10, NSE (neuron specific enolase) in T9 and T10, pain score in T8, T9 and T10 after operation.

Conclusion: the application of Dex in the resection of intracranial aneurysms can protect the brain of patients, minimize the influence of operation on hemodynamics, and relieve postoperative pain, which is worthy of clinical application.
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http://dx.doi.org/10.1016/j.sjbs.2020.03.027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658695PMC
July 2020

Neuroprotective effects of soy isoflavones on chronic ethanol-induced dementia in male ICR mice.

Food Funct 2020 Nov;11(11):10011-10021

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China.

Chronic ethanol intake can lead to dementia by activating neuroinflammation, causing oxidative stress response, reducing cholinergic function and inducing neuronal apoptosis. Soy isoflavones (SIs) exert beneficial effects in a variety of neurodegenerative disorders by acting on the anti-inflammatory, anti-oxidant, anti-apoptotic and neuro-trophic processes. However, at present, it is unknown whether SIs have a neuroprotective effect in chronic ethanol-induced dementia. The aim of the present study was to investigate the effect of SI on chronic ethanol-induced cognitive deficit in mice and explore the underlying mechanisms. The cognition-impaired mouse model was induced by ethanol (2.0 g kg-1, p.o) for 4 weeks. SIs (10, 20 or 40 mg kg-1, p.o) were delivered 1 hour after ethanol administration for 4 weeks. The Morris water maze (MWM) test and the passive avoidance (PA) task were conducted to evaluate the learning and memory abilities. After the behavioral tests, the biochemical parameter assay and western blot analysis were used to explore the underlying mechanisms of its action. SI administration significantly improved the cognitive performance in the MWM and PA tests, regulated the acetylcholinesterase (AChE) activity and acetylcholine (Ach) level, elevated the synaptic plasticity-related protein expressions and inhibited neuron apoptosis-related protein expressions in the cortex and hippocampus of mice. The results revealed that soy isoflavones may provide a possible novel candidate for the prevention and treatment of alcoholic dementia.
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http://dx.doi.org/10.1039/d0fo02042gDOI Listing
November 2020

[Clinical study of ultrasound evaluation of fiberoptic bronchoscope guided tracheotomy].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2020 Aug;32(8):1013-1015

Department of Critical Care Medicine, Donge Hospital, Donge 252200, Shandong, China. Corresponding author: Geng Xihua, Email:

Objective: To explore the clinical effect of ultrasound evaluation of fiberoptic bronchoscope (FB) guided tracheotomy, which can provide help for difficult tracheotomy and new operators.

Methods: The operating protocol was standardized for ultrasound evaluation of FB guided tracheotomy. Ten patients with difficult tracheotomy admitted to the department of critical care medicine of Donge Hospital from October 2019 to January 2020 were enrolled. According to this protocol, FB guided tracheotomy was performed under the ultrasound evaluation, and the amount of blood loss, operation time and related complications during procedures were collected.

Results: The preparation of supplies, personnel, patients and the operation, the process of FB guided tracheotomy under the ultrasound evaluation were standardized. When tracheotomy was preformed for patients with difficult tracheotomy, it was necessary to use ultrasound first to evaluate the neck condition and vascular disorientation of patients, and the tracheotomy plan (tracheotomy site, incision size, and incision depth) was designed, and then the tracheotomy process was monitored under the guidance of FB. Among the 10 patients with difficult tracheotomy, 6 were male and 4 were female; body mass index was (32.2±1.4) kg/m. Tracheotomy was successfully completed within 10 minutes in all the 10 patients, with less than 5 mL blood loss, and no complications occurred.

Conclusions: Ultrasound evaluation of FB guided tracheotomy can improve the clinical operations and ensure patient safety.
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http://dx.doi.org/10.3760/cma.j.cn121430-20200116-00060DOI Listing
August 2020

Prognostic role of tumor-infiltrating lymphocytes in gastric cancer: a meta-analysis and experimental validation.

Arch Med Sci 2020 9;16(5):1092-1103. Epub 2019 Sep 9.

Institute of Cancer Research, School of Basic Medical Science, Xi'an Jiaotong University Xi'an, China.

Introduction: We performed a meta-analysis and an experimental validation to investigate the association between tumor infiltrating lymphocytes (TILs) and the outcome of gastric cancer (GC) patients to provide prognostic indicators for clinical practice.

Material And Methods: The relative literature of TILs in tumor tissue from patients with gastric cancer was searched from PubMed, Embase, NIH databases, from April 2000 to 31 December 2016. Studies on the prognostic value of TILs as CD3+, CD4+, CD8+, GrB+, and FOXP3+ lymphocytes for GC were retrieved, and also the related references were traced as supplements. Independent screening documents, extracting information and evaluating quality were implemented independently by 2 evaluators according to the inclusion and exclusion criteria, which were then analyzed by meta-analysis using STATA version 12.0 software.

Results: The results indicated that high levels of intratumoral CD8+, CD3+ and CD4+ T cell infiltration were associated with better overall survival(OS) in gastric cancer patients, while high density of intratumoral FOXP3+ T cells was not closely associated with a worse outcome. Additionally, in our study, higher density of granzyme B+ (GrB+) T cell infiltration indicated an optimistic prognosis, and infiltration of a larger number of general TILs also suggested a favorable prognosis by log-rank test analysis.

Conclusions: This meta-analysis clarified that high levels of CD8+, CD3+, and CD4+ T cell infiltration in tumor tissue showed better OS in GC patients, whereas high density of FOXP3+ T cell infiltration may not be recognized as a negative prognostic factor. These results may provide some useful prognostic indicators for clinical application in gastric cancer.
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http://dx.doi.org/10.5114/aoms.2019.86101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444703PMC
September 2019

[Correlation between peripheral blood Th17 cells and carotid intima-media thickness in OSA patients].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2020 Aug;34(8):747-751

Department of Otorhinolaryngology Head and Neck Surgery,the Puai Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430034,China.

To study the carotid intima-media thickness (IMT) level of OSA patients of different severity, the proportion of Th17 cells in peripheral blood and the mRNA level of nuclear transfer factor RORγt, and to explore the relationship between Th17 cells and atherosclerosis in OSA patients. Sixty-two patients who underwent respiratory and sleep monitoring were selected and divided into three groups according to the AHI index: 15 patients in the normal control group(AHI<5), there were 22 cases in the mild group(AHI 5-15) and 25 cases in the moderate and severe group(AHI≥15). Carotid intima-media thickness(IMT) was measured in all subjects, the ratio of Th17 cells in peripheral blood monouelear cells(PBMC) were analysed by flow cytometry. The expression of RORγt mRNA were detected by real-time polymerase chain reaction. The carotid IMT of patients in the normal control group, the mild OSA group and the moderate to severe OSA group were (0.74±0.21) mm, (1.09±0.23) mm and (1.60±0.30) mm, respectively. The moderate to severe group was higher than the mild group and the normal control group, the difference was statistically significant (<0.01). The proportion of Th17 in peripheral blood of the three groups was(2.54±0.20)%, (4.34±0.30)%, and (8.27±0.31)%, respectively. The moderate to severe group was significantly higher than the mild group and the control group (<0.01), the difference was statistically significant. The relative mRNA expression levels of RORγt in the three groups were 0.92±0.24, 2.60±0.59, and 4.93±0.72, respectively. The moderate to severe group was significantly higher than the mild group and the control group (<0.01), the difference was statistically significant. The proportion of Th17 in peripheral blood and the relative expression of RORvt mRNA were positively correlated with the carotid IMT ( value was 0.80, 0.78, respectively, all <0.01). Th17 cells differentiation is increased in OSA patients, and Th17 cells are correlated with indicators reflecting the progression of atherosclerosis. Th17 cells may be involved in the development of atherosclerosis in OSA patients.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2020.08.016DOI Listing
August 2020

Effect of lignin-based monomer on controlling the molecular weight and physical properties of the polyacrylonitrile/lignin copolymer.

Int J Biol Macromol 2020 Dec 15;164:2312-2322. Epub 2020 Aug 15.

State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Pulp & Paper Science and Technology of Shandong Province/Ministry of Education, Qilu University of Technology, Shandong Academy of Sciences, Jinan, China, 250353.

In this work, lignin was grafted with acrylonitrile to control the molecular weights and molecular architecture of polyacrylonitrile (PAN)/lignin copolymer. Lignin-acrylonitrile monomer (LA-AN) and its copolymers with AN were synthesized successfully. First, lignin was aminated (LA) and then grafted with 2-chloroacrylonitrile to prepare LA-AN. The copolymerization of LA-AN and AN was carried out using 2,2-azobis(2-methylpropionitrile) as initiator. The modification, grafting, and copolymerization were confirmed with Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and X-ray photoelectron spectroscopy. Contrary to the previous studies, gel permeation chromatography showed that the molecular weight of the copolymers was increased significantly due to the presence of lignin (up to 203,944). Viscosity analysis revealed that the addition of lignin reduces the viscosity of the copolymer solution. While thermogravimetric analysis showed improvement in the degradation temperature, and lowering of the melt temperature, as revealed by differential scanning calorimetry. These findings indicated that the attaching acrylonitrile on lignin molecules result in control of the molecular weight and molecular structure of PAN/Lignin copolymers which results in enhanced solubility, spinnability, and other properties associated with molecular weight.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.119DOI Listing
December 2020

FLAD1 is up-regulated in Gastric Cancer and is a potential prediction of prognosis.

Int J Med Sci 2020 6;17(12):1763-1772. Epub 2020 Jul 6.

Department of Pathology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou510000, P. R. China.

Gastric cancer (GC) is a common malignancy throughout the world. Biomarkers for prognosis and risk evaluation of GC are rapidly discovered. We investigated the prognostic role of FLAD1, an important protein-coding gene that affects cell cycle and survival. The expression of FLAD1 at mRNA levels in GC tumor tissues and normal tissues was mined and analyzed in Oncomine database and verified in 10 pairs of GS tissues and their adjacent normal tissues in our center by RT qPCR. The FLAD1 protein expression were detected in 106 paraffin-embedded GC tissues by immunohistochemistry (IHC). Statistical analyses were applied to evaluate the clinical significance of FLAD1. The prognostic value of FLAD1 mRNA expression was also analyzed using the Kaplan-Meier plotter (www.kmplot.com). Statistics obtained from online database suggested FLAD1 mRNA was overexpressed in GC tissues. The results were further validated in 10 pairs of GS tissues and adjacent normal tissues in our center (p=0.021). IHC and survival analysis of GC samples from 106 patients showed FLAD1 was overexpressed in 63/106 (59.4%) patients and was associated to higher TNM stage (p=0.026). Multivariate analysis revealed FLAD1 was an independent prognostic factor for GC (p < 0.001). Furthermore, FLAD1 mRNA was associated to unfavorable overall survival (OS), first progression (FP), and post-progression survival (PPS) of GC (p<0.001). FLAD1 in GC is overexpressed at both mRNA and protein level and could be a potential biomarker for GC prognosis.
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http://dx.doi.org/10.7150/ijms.48162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378663PMC
July 2020

Transient sublethal hypoxia in neonatal rats causes reduced dendritic spines, aberrant synaptic plasticity, and impairments in memory.

J Neurosci Res 2020 08 3;98(8):1588-1604. Epub 2020 Jun 3.

Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Hypoxic/ischemic insult, a leading cause of functional brain defects, has been extensively studied in both clinical and experimental animal research, including its etiology, neuropathogenesis, and pharmacological interventions. Transient sublethal hypoxia (TSH) is a common clinical occurrence in the perinatal period. However, its effect on early developing brains remains poorly understood. The present study was designed to investigate the effect of TSH on the dendrite and dendritic spine formation, neuronal and synaptic activity, and cognitive behavior of early postnatal Day 1 rat pups. While TSH showed no obvious effect on gross brain morphology, neuron cell density, or glial activation in the hippocampus, we found transient hypoxia did cause significant changes in neuronal structure and function. In brains exposed to TSH, hippocampal neurons developed shorter and thinner dendrites, with decreased dendritic spine density, and reduced strength in excitatory synaptic transmission. Moreover, TSH-treated rats showed impaired cognitive performance in spatial learning and memory. Our findings demonstrate that TSH in newborn rats can cause significant impairments in synaptic formation and function, and long-lasting brain functional deficits. Therefore, this study provides a useful animal model for the study of TSH on early developing brains and to explore potential pharmaceutical interventions for patients subjected to TSH insult.
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http://dx.doi.org/10.1002/jnr.24652DOI Listing
August 2020

Simultaneous identification of animal-derived components in meats using high-throughput sequencing in combination with a custom-built mitochondrial genome database.

Sci Rep 2020 06 2;10(1):8965. Epub 2020 Jun 2.

State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.

Currently, the inspection and supervision of animal ingredients relies primarily upon specific amplification-dependent methods, whose efficiency and accuracy are being seriously challenged by the increasing diversity and complexity of meat products. High-throughput sequencing (HTS) technology was employed to develop an alternative method to detect animal-derived ingredients in meat products. A custom-built database containing 2,354 complete mitochondrial genomic sequences from animals, an identification analysis pipeline based on short-sequence alignment, and a web-based server were built to facilitate this detection. The entire process, including DNA extraction, gene amplification, and sequencing, was established and optimized for both marker gene (part of the CYTB gene)-based detection and total DNA-based detection. Using simulated samples containing various levels of pig, cattle, sheep, chicken, rabbit, and mice ingredients, the detection capability and accuracy of this method were investigated. The results of this study indicated that the method is capable of detecting animal components in meats that are present at levels as low as 1%. Our method was then tested using 28 batches of real meat products such as raw meat slices, raw meat mince, cooked dried meat, cooked meat sausage, and other supermarket samples, with a traditional qPCR method as the control. The results demonstrated an accuracy of 97.65% for the qualitative detection method, which indicate that the developed method is reliable for the detection of animal components. The method is also effective for the identification of unknown food samples containing mixed animal components, which suggests a good future in application.
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http://dx.doi.org/10.1038/s41598-020-65724-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7265478PMC
June 2020

The special immune microenvironment of tumor budding and its impact on prognosis in gastric adenocarcinoma.

Pathol Res Pract 2020 Jun 17;216(6):152926. Epub 2020 Mar 17.

Institute for Cancer Research, School of Basic Medical Science, Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. Electronic address:

Recent studies showed that the tumor-infiltrating lymphocytes (TILs) are not randomly distributed, but organized to accumulate more or less densely in different regions within tumors, which have provoked new thoughts on cancer management. In this study we explored the characteristics of tumor immunemicroenvironment (TIME) for the tumor budding (TB) and lymphocytes in patients with gastric adenocarcinoma (GAC) as well as their prognostic significance. The TILs around the TB at the invasive margin were assessed by double-immunohistochemistry staining for the CD8, FOXP3, OX40 and GrB phenotypes. Results showed that there was a negative correlation between the density of TB and TILs in the budding area, tumor stroma and parenchyma. And the number of TILs around the TB was evidently reduced, compared with TILs in the non-budding region (P < 0.001). Additionally, the number of TILs in turn changed from non-budding area CD8+>FOXP3+>OX40+> GrB + T cells to FOXP3+>CD8+>OX40 + T > GrB + T cells in budding area. Survival rate was significantly lower in patients who had a higher density of TB (P < 0.001) and a lower density of TILs (P = 0.013). We concluded that TB was surrounded by a weak immune surveillance and immunosuppressive response supported the spatial heterogeneity in the TIME of gastric adenocarcinomas. The regional heterogeneity should be attached importance for identifying the influence of the TIME on cancer development and evolution.
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http://dx.doi.org/10.1016/j.prp.2020.152926DOI Listing
June 2020

Protective effects of Genistein on the cognitive deficits induced by chronic sleep deprivation.

Phytother Res 2020 Apr 2;34(4):846-858. Epub 2020 Mar 2.

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

Sleep deprivation has been widely reported to cause cognitive dysfunction, and elevation in oxidative stress and inflammation in the body, including the brain, have been suggested as the main factors. Genistein (GE) is an isoflavone widely present in leguminous plants, and it was found to exert a wide spectrum of biological activities, including antioxidant, anti-inflammatory, hepatoprotective, neuroprotective, and antimetastatic effects. In this study, the protective effect of GE on chronic sleep deprivation (CSD)-induced cognitive dysfunction was investigated. The mice were subjected to the sleep interruption apparatus and continuously sleep deprived for 25 days. GE was orally administrated (10, 20, and 40 mg/kg) during the sleep deprivation process totally for 25 days. Cognitive behavioral tests were conducted to study the learning and memory using the object location recognition (OLR) task, novel object recognition (NOR) test, and the Morris water maze (MWM) task. Additionally, the cortex and hippocampus were dissected to measure the oxidative stress markers and the antioxidant element nuclear erythroid-2-related factor 2 (Nrf2) and its downstream targets, including glutamate cysteine ligase catalytic, glutamate cysteine ligase modifier, heme oxygenase 1, and quinone oxidoreductase 1, as well as nuclear factor kappa B (NF-κB) p65, nitric oxide synthase (iNOS), and cyclooxygenase 2 (COX-2) protein expression. Moreover, the pro-inflammatory cytokines (TNF-α, interleukin [IL]-6, and IL-1β) level was examined in the serum. The current results showed that GE could dose-dependently ameliorate the cognitive deficits of CSD-treated mice in the OLR, NOR, and MWM tasks. In addition, GE treatment significantly elevated the activities of total antioxidant capacity and superoxide dismutase and the level of glutathione and lowered the content of malondialdehyde in the cortex and hippocampus of CSD-treated mice. Furthermore, GE administration effectively activated the antioxidant element Nrf2 and its downstream targets in the cortex and hippocampus of CSD-treated mice. Moreover, GE treatment significantly suppressed CSD-induced NF-κB p65, iNOS, and COX-2 activation in the cortex and hippocampus, as well as inhibited CSD-induced pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) release in the serum. Taken together, all these results suggested that GE has substantial potential as a therapeutic intervention for the alleviation of CSD-induced deleterious effects.
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http://dx.doi.org/10.1002/ptr.6567DOI Listing
April 2020

Caffeic acid assists microwave heating to inhibit the formation of mutagenic and carcinogenic PhIP.

Food Chem 2020 Jul 19;317:126447. Epub 2020 Feb 19.

School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region.

The inhibitory effect of caffeic acid on the formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP) was investigated in chemical model systems under microwave heating (MW). A mechanistic study was subsequently carried out to identify the inhibitory mechanism. The results showed that both for conductive heating (CV) and MW, the inhibition of PhIP increased with the concentration of caffeic acid but decreased with the prolongation of heating time. The inhibition on PhIP under MW was always higher than under CV, which were dominated by the difference in dielectric loss (ε″). UPLC-MS analysis showed that caffeic acid releases a CO molecule to produce 4-vinylcatechol which can form adducts with phenylacetaldehyde, thus reducing its availability for PhIP formation. The structure of adduct was characterized as 3-(3,4-dihydroxyphenyl)-2-phenylbutanal with a molecular weight of 256. The findings indicate that trapping of phenylacetaldehyde by 4-vinylcatechol is a key mechanism of caffeic acid in inhibiting PhIP formation.
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http://dx.doi.org/10.1016/j.foodchem.2020.126447DOI Listing
July 2020

The orexinergic neural pathway from the lateral hypothalamus to the nucleus accumbens and its regulation of palatable food intake.

Neuropeptides 2020 Apr 8;80:102028. Epub 2020 Feb 8.

Pathophysiology Department, School of Basic Medicine, Qingdao University, Qingdao, Shandong, China. Electronic address:

Objective: To explore the orexinergic pathway from the lateral hypothalamus (LHA) to the nucleus accumbens (NAc) and its regulation on the palatable food intake.

Methods: Fluorescent gold retrograde tracing combined with fluoro-immunohistochemical staining were used to observe the projection of orexinergic neurons from LHA to NAc. The orexin-A expression in LHA and c-Fos in NAc were studied after electrical stimulation of LHA. The firing rates of neurons were monitored by single-unit extracellular electric discharge recording and the palatable food intake were measured after orexin microinjection in NAc or electrical stimulation of LHA.

Results: (1) Fluorescent gold retrograde tracing combined with fluoro-immunohistochemical staining showed some orexinergic neural projection from the LHA to the NAc shell. (2) Electrical stimulation of LHA significantly enhanced the expression of orexin-A in LHA and the expression of c-Fos in NAc (P < .05). (3) The results of single-unit extracellular discharge recording showed that the microinjection of orexin in NAc or electrical stimulation of LHA significantly increased the discharge activity of gastric distension responsive neurons in NAc, and the effect could be partly blocked by pretreatment of orexin-A receptor inhibitor SB334867 in NAc (P < .05). (4) Microinjection orexin-A in NAc or electrical stimulation of LHA significantly increased the palatable food intake in rats, and the effect also was partly inhibited by pretreatment of SB334867 in NAc (P < .05).

Conclusion: There is an orexinergic pathway from LHA to NAc, which may have potential regulatory effects on food reward and obesity.
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http://dx.doi.org/10.1016/j.npep.2020.102028DOI Listing
April 2020

Comparing the performance of conventional PCR, RTQ-PCR, and droplet digital PCR assays in detection of Shigella.

Mol Cell Probes 2020 06 13;51:101531. Epub 2020 Feb 13.

Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, 400715, Chongqing, China. Electronic address:

The incidence of foodborne infections caused by Shigella spp. is still very high in every year, which poses a great potential threat to public health. Conventional quantification methods based on culture techniques, biochemical, and serological identification are time-consuming and labor-intensive. To develop a more rapid and efficient detection method of Shigella spp., we compared the sensitivity and specificity of three different polymerase chain reaction (PCR) methods, including conventional PCR, quantitative real-time PCR (RTQ-PCR), and droplet digital PCR (ddPCR). Our results indicated that ddPCR method exhibited higher sensitivity, and the limit of detection was 10 ng/μl for genomic DNA templates, 10 cfu/ml for Shigella bacteria culture. In addition, we found that ddPCR was a time-saving method, which required a shorter pre-culturing time. Collectively, ddPCR assay was a reliable method for rapid and effective detection of Shigella spp.
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http://dx.doi.org/10.1016/j.mcp.2020.101531DOI Listing
June 2020

iTRAQ-Based Comparative Proteomic Analysis of ADP1 Under DNA Damage in Relation to Different Carbon Sources.

Front Microbiol 2019 14;10:2906. Epub 2020 Jan 14.

School of Environment, Tsinghua University, Beijing, China.

DNA damage response allows microorganisms to repair or bypass DNA damage and maintain the genome integrity. It has attracted increasing attention but the underlying influential factors affecting DNA damage response are still unclear. In this work, isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic analysis was used to investigate the influence of carbon sources on the translational response of ADP1 to DNA damage. After cultivating in a nutrient-rich medium (LB) and defined media supplemented with four different carbon sources (acetate, citrate, pyruvate, and succinate), a total of 2807 proteins were identified. Among them, 84 proteins involved in stress response were significantly altered, indicating the strong influence of carbon source on the response of ADP1 to DNA damage and other stresses. As the first study on the comparative global proteomic changes in ADP1 under DNA damage across nutritional environments, our findings revealed that DNA damage response in ADP1 at the translational level is significantly altered by carbon source, providing an insight into the complex protein interactions across carbon sources and offering theoretical clues for further study to elucidate their general regulatory mechanism to adapt to different nutrient environments.
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http://dx.doi.org/10.3389/fmicb.2019.02906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971185PMC
January 2020

Increased High-Risk Human Papillomavirus Viral Load Is Associated With Immunosuppressed Microenvironment and Predicts a Worse Long-Term Survival in Cervical Cancer Patients.

Am J Clin Pathol 2020 03;153(4):502-512

Institute for Molecular Radiobiology of Cancer, First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China.

Objectives: To evaluate the correlation between tumor-infiltrating lymphocytes (TILs) and the viral load of high-risk human papillomavirus (HR-HPV) in cervical cancer patients.

Methods: A total of 62 cervical cancer patients were recruited during 1993-1994 and assigned into four groups treated with radiotherapy alone or radiotherapy combined with chemotherapy and/or thermotherapy. Ki67+ tumor cells, CD4+, CD8+, FoxP3+, OX40+ and granzyme B+ TILs were detected by immunohistochemistry. The viral load of HR-HPV in biopsy tissues before therapy was detected by in situ hybridization.

Results: The patients with high HPV viral load showed a significantly lower 15-year survival rate and an advanced International Federation of Gynecology and Obstetrics (FIGO) stage and increased recurrence rate. The distribution of Ki67+ tumor cells, FoxP3+ TILs, and CD8+/FoxP3+ ratio was obviously different between low and high HPV viral load groups. A worse clinical outcome was also implicated with increased HPV viral load tested by Cox regression analysis.

Conclusions: Patients with increased HR-HPV viral load tend to be resistant to therapy with decreased immune surveillance in the immune microenvironment. Thus, HR-HPV viral load would influence the local immune microenvironment, and then further affect the survival of cervical cancer patients.
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http://dx.doi.org/10.1093/ajcp/aqz186DOI Listing
March 2020

Label-assisted chemical adsorption triggered conversion of electroactivity of sensing interface to achieve the Ag/AgCl process for ultrasensitive detection of CA 19-9.

Anal Chim Acta 2020 Jan 25;1093:43-51. Epub 2019 Sep 25.

Department of Chemistry, Capital Normal University, Beijing, 100048, China. Electronic address:

Efficient strategies in enhancing sensitivity are pivotal to ultrasensitive detection of tumor markers. In this work, based on the strategy of label-assisted chemical adsorption triggered conversion of electroactivity of sensing interface, a Ag/AgCl process was achieved to enhance sensitivity of the constructed sandwich-type amperometric immunosensor for ultrasensitive detection of carbohydrate antigen 19-9 (CA19-9). Briefly, polydopamine-Ag nanoparticles (PDA-Ag NPs), as signal precursor, combined with labeling antibody were served as labels and graphene oxide-melamine (GO-MA) substrate with chemical absorption capacity was applied as smart sensing interface. After successfully incubating labels, there was primitively no current response due to the poor conductivity between labels and electrode. However, in the presence of HO, Ag NPs from labels can be etched into Ag ions, which were adsorbed by GO-MA to form GO-MA-Ag as electroactive substrate. Then, the substrate exhibited a sharp and stable electrochemistry peak of solid-state Ag/AgCl process in the buffer containing KCl. The sensitivity toward detection of CA19-9 was notably enhanced based on the appearance of sharp peak. Under optimum conditions, the designed immunosensor demonstrated a wide working range from 0.0001 to 100 U mL and an ultralow detection limit 0.032 mU mL. Thus, utilizing this strategy to construct immunosensor was highly promising in clinical diagnosis for ultrasensitive detection of tumor makers.
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http://dx.doi.org/10.1016/j.aca.2019.09.061DOI Listing
January 2020