Publications by authors named "Nana Liu"

82 Publications

Hippocampal transcriptome-wide association study and neurobiological pathway analysis for Alzheimer's disease.

PLoS Genet 2021 Feb 25;17(2):e1009363. Epub 2021 Feb 25.

Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin, China.

Genome-wide association studies (GWASs) have identified multiple susceptibility loci for Alzheimer's disease (AD), which is characterized by early and progressive damage to the hippocampus. However, the association of hippocampal gene expression with AD and the underlying neurobiological pathways remain largely unknown. Based on the genomic and transcriptomic data of 111 hippocampal samples and the summary data of two large-scale meta-analyses of GWASs, a transcriptome-wide association study (TWAS) was performed to identify genes with significant associations between hippocampal expression and AD. We identified 54 significantly associated genes using an AD-GWAS meta-analysis of 455,258 individuals; 36 of the genes were confirmed in another AD-GWAS meta-analysis of 63,926 individuals. Fine-mapping models further prioritized 24 AD-related genes whose effects on AD were mediated by hippocampal expression, including APOE and two novel genes (PTPN9 and PCDHA4). These genes are functionally related to amyloid-beta formation, phosphorylation/dephosphorylation, neuronal apoptosis, neurogenesis and telomerase-related processes. By integrating the predicted hippocampal expression and neuroimaging data, we found that the hippocampal expression of QPCTL and ERCC2 showed significant difference between AD patients and cognitively normal elderly individuals as well as correlated with hippocampal volume. Mediation analysis further demonstrated that hippocampal volume mediated the effect of hippocampal gene expression (QPCTL and ERCC2) on AD. This study identifies two novel genes associated with AD by integrating hippocampal gene expression and genome-wide association data and reveals candidate hippocampus-mediated neurobiological pathways from gene expression to AD.
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http://dx.doi.org/10.1371/journal.pgen.1009363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7906391PMC
February 2021

General Programmable Growth of Hybrid Core-Shell Nanostructures with Liquid Metal Nanodroplets.

Adv Mater 2021 Feb 1:e2008024. Epub 2021 Feb 1.

Institute for Superconducting and Electronic Materials (ISEM), Australian Institute for Innovative Materials (AIIM), University of Wollongong, Wollongong, NSW, 2500, Australia.

Core-shell and hollow nanostructures have been receiving significant interest due to their potential in wide scientific and technological fields. Given such large scope, however, they still lag far behind in terms of the ambition toward controllably, or even programmatically, synthesizing libraries of core-shell structures on a large scale. Here, a general route for the programmable preparation of complex core-shell nanostructures by using liquid metal (LM) droplets as reformable templates is presented, and the triggering of a localized galvanic replacement reaction in one ultrasonication system is demonstrated. Benefiting from the activity and mobility of the metal components in LM templates, high-level compositional diversity control and quantitative regulation of both the core and the shell layers of the heterogeneous products are achieved, which cannot be realized with a solid-template synthetic route.
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http://dx.doi.org/10.1002/adma.202008024DOI Listing
February 2021

The effects of fipronil exposure on oxidative stress, non-specific immunity, autophagy, and apoptosis in the common carp.

Environ Sci Pollut Res Int 2021 Jan 30. Epub 2021 Jan 30.

College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.

The increase in the area treated with the insecticide fipronil has caused concern for aquatic organisms such as fish. Here, we assessed the effect of fipronil on carp indexes of non-specific immunity, oxidative stress, autophagy, and apoptosis following exposure to 0.074 mg/L and 0.185 mg/L of fipronil in the aqueous environment for 1 day, 3 days, 5 days, and 7 days. It was found that glutathione (GSH), malonaldehyde (MDA), and superoxide dismutase (SOD) in gills were significantly reduced (P < 0.05). The increase in exposure time increases the impact on GSH, SOD, and MDA parameters in the liver and intestine. Liver acid phosphatase (ACP), alkaline phosphatase (AKP), and lysozyme (LZM) activity levels increased significantly in the treatment group on the first day after exposure, except for the 0.074 mg/L group of ACP (P < 0.05). The mRNA expression levels of autophagy-related genes ATG12, ATG5, ATG16L, LC3-II, and BECN1 were generally elevated in the liver and intestine during the initial exposure period (P < 0.05), while mTOR was significantly reduced on the first and third days after treatment (P < 0.05). From the results of Western blotting (WB), we can see that the amount of LC3-II was significantly higher than that of LC3-I at 1, 3, and 5 days of exposure (P < 0.05). Furthermore, the apoptosis-related gene Bcl-2 reached its peak in the liver, intestine, and gill on the first day, and caspase3 was significantly downregulated throughout the exposure period (P < 0.05). The results showed that fipronil was potentially harmful to carp and should be used moderately to reduce the damage to aquatic ecosystems. This study complements the mechanism theory of fipronil on fish toxicology and has a certain value for human health risk assessment.
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http://dx.doi.org/10.1007/s11356-021-12573-xDOI Listing
January 2021

Selenium bioavailability in soil-wheat system and its dominant influential factors: A field study in Shaanxi province, China.

Sci Total Environ 2021 Jan 20;770:144664. Epub 2021 Jan 20.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China. Electronic address:

Selenium (Se) content of crops depends on the local soil Se content and/or its bioavailability, and identifying the influence factors of soil Se bioavailability is a significant basis for adopting targeted agronomic measures to improve the Se nutritional status of humans. In this study, the main wheat-producing region in Shaanxi province with similar parent material and climate conditions was selected as the study area. The total Se contents of 602 soil samples and their corresponding wheat grains were determined, and the distribution characteristics of soil Se bioavailability and its dominant influential factors were investigated. Results showed that the total Se content ranged from 0.02 mg/kg to 1.67 mg/kg (average of 0.25 ± 0.25 mg/kg) in soil, which was lower than that content in China (0.29 mg/kg). The Se content of wheat grain was 0.001-1.50 mg/kg (average of 0.11 ± 0.19 mg/kg). The distribution trend of the Se content in wheat grains was different from that of the total soil Se, but it was consistent with the distribution of soil bioavailable Se content. The bioavailable Se accounted for 11.1% of the total soil Se. This could be attributed to relatively high soil Se bioavailability of the study area belonging to alkaline soil (with a pH of approximately 8). Both redundancy analysis and path analysis revealed that soil pH and organic matter were the dominant influential factors of soil Se bioavailability in Shaanxi wheat-producing area, and the soil Se bioavailability increased with these two parameters raising. On this basis, a prediction model was established to predict the Se content in wheat grain. The results show that the various agronomic measures could be used to produce Se-enriched wheat by regulating the soil pH and the organic matter content in Se biofortification practice.
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http://dx.doi.org/10.1016/j.scitotenv.2020.144664DOI Listing
January 2021

Clinical characteristics of severe influenza virus infects lower respiratory tract in children.

Panminerva Med 2021 Jan 4. Epub 2021 Jan 4.

Hospital Infection Management Department, Maternal and Child Health Care Hospital of Dongchangfu District, Liaocheng, China -

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http://dx.doi.org/10.23736/S0031-0808.20.04183-XDOI Listing
January 2021

An association study between MiR-146a and INSR gene polymorphisms and hypertensive disorders of pregnancy in Northeastern Han Chinese population.

Placenta 2021 Jan 5;104:94-101. Epub 2020 Dec 5.

Department of Ultrasound, Shenzhen University General Hospital, NO.1098, Xueyuan Avenue, Nanshan District, Shenzhen, 518055, Guangdong, China. Electronic address:

Introduction: Hypertensive disorders of pregnancy(HDP) is a complex and challenging group of pregnancy complications that is one of the leading causes of maternal and fetal death worldwide. Recent studies have shown that the single nucleotide polymorphism(SNP) may play a role in the pathogenesis of HDP. This study aimed to investigate the association of MiR-146a rs2910164 and insulin receptor(INSR) rs2059806 SNPs with HDP and their associated complications in the Han population of Northeast China.

Methods: A total of 240 HDP patients and 380 healthy controls were selected for genotype determination. For the most special and high incidence of HDP, we also studied the SNPs in association with pre-eclampsia(PE) patients. In addition, HDP complicated with gestational diabetes mellitus(GDM) patients was further analyzed to identify the association between SNPs and HDP-related complications. Multivariate logical regression analysis combined with 10, 000 permutation test corrections was used to analyze the association of MiR-146a and INSR SNPs with HDP.

Results: After adjusting for relevant factors, MiR-146a rs2910164 or INSR rs2059806 SNPs were not significantly different between HDP or PE patients and healthy controls(P>0.05). Meanwhile, MiR146a rs2910164 and INSR rs2059806 SNPs were not significantly different between HDP complicated with GDM and control group.

Discussion: Our data indicates that MiR-146a rs2910164 and INSR rs2059806 SNPs may not be significantly related with HDP in the Han population of Northeast China living in Heilongjiang Province.
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http://dx.doi.org/10.1016/j.placenta.2020.11.011DOI Listing
January 2021

Prefrontal Granule Cell-Related Genes and Schizophrenia.

Cereb Cortex 2020 Dec 4. Epub 2020 Dec 4.

Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin 300052, China.

Although both the granular layer of the prefrontal cortex (PFC) and schizophrenia are unique in primates, especially humans, their linkage is unclear. Here, we tested whether schizophrenia is associated with expression profiles of the granule cell (GC)-related genes in the human PFC. We identified 14 candidate GC-related genes with gradually increased expression levels along the gradient of the agranular, dysgranular, light-granular, and granular prefrontal regions based on the densely sampled gene expression data of 6 postmortem human brains, and with more than 10-fold expression in neurons than other cell types based on the single-cell RNA-sequencing data of the human PFC. These GC-related genes were functionally associated with synaptic transmission and cell development and differentiation. The identified 14 GC-related genes were significantly enriched for schizophrenia, but not for depression and bipolar disorder. The expression levels of the 4 stable schizophrenia- and GC-related genes were spatially correlated with gray matter volume differences in the PFC between patients with schizophrenia and healthy controls. This study provides a set of candidate genes for the human prefrontal GCs and links expression profiles of the GC-related genes to the prefrontal structural impairments in schizophrenia.
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http://dx.doi.org/10.1093/cercor/bhaa360DOI Listing
December 2020

Sirt7-p21 Signaling Pathway Mediates Glucocorticoid-Induced Inhibition of Mouse Neural Stem Cell Proliferation.

Neurotox Res 2021 Apr 6;39(2):444-455. Epub 2020 Oct 6.

Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092, China.

Prenatal glucocorticoid (GC) overexposure impacts fetal hippocampal neural stem cells (NSCs) and increases the risk for relative cognitive and mood disorders in offspring. However, the precise underlying mechanisms remain elusive. Here, we treated mouse hippocampal NSCs with dexamethasone (DEX) in vitro and found that DEX inhibited cell proliferation and Sirt7 expression. In addition, prenatal mouse overexposure to DEX induced the suppression of Sirt7 in the hippocampus of offspring. Sirt7 knockdown significantly decreased the percentage of proliferating cells but did not further reduce the NSC proliferation rate in the presence of DEX, whereas Sirt7 overexpression rescued DEX-induced inhibition of hippocampal NSC proliferation. Moreover, DEX inhibited Sirt7 expression through the glucocorticoid receptor (GR), and p21 was found to mediate the functional effect of DEX-induced Sirt7 suppression. In conclusion, our data demonstrate for the first time the effect of DEX on the Sirt7-p21 pathway in hippocampal NSCs, identifying a new potential therapeutic target for prenatal GC overexposure-related neurodevelopmental disorders in offspring.
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http://dx.doi.org/10.1007/s12640-020-00294-xDOI Listing
April 2021

Critical regulation of a NDIME/MEF2C axis in embryonic stem cell neural differentiation and autism.

EMBO Rep 2020 11 5;21(11):e50283. Epub 2020 Oct 5.

Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Tongji University, Shanghai, China.

A microdeletion within human chromosome 5q14.3 has been associated with the occurrence of neurodevelopmental disorders, such as autism and intellectual disability, and MEF2C haploinsufficiency was identified as main cause. Here, we report that a brain-enriched long non-coding RNA, NDIME, is located near the MEF2C locus and is required for normal neural differentiation of mouse embryonic stem cells (mESCs). NDIME interacts with EZH2, the major component of polycomb repressive complex 2 (PRC2), and blocks EZH2-mediated trimethylation of histone H3 lysine 27 (H3K27me3) at the Mef2c promoter, promoting MEF2C transcription. Moreover, the expression levels of both NDIME and MEF2C were strongly downregulated in the hippocampus of a mouse model of autism, and the adeno-associated virus (AAV)-mediated expression of NDIME in the hippocampus of these mice significantly increased MEF2C expression and ameliorated autism-like behaviors. The results of this study reveal an epigenetic mechanism by which NDIME regulates MEF2C transcription and neural differentiation and suggest potential effects and therapeutic approaches of the NDIME/MEF2C axis in autism.
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http://dx.doi.org/10.15252/embr.202050283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645248PMC
November 2020

Influence of processing methods and exogenous selenium species on the content and in vitro bioaccessibility of selenium in Pleurotus eryngii.

Food Chem 2021 Feb 24;338:127661. Epub 2020 Jul 24.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture, Yangling, Shaanxi 712100, China. Electronic address:

Understanding the effects of processing on the Se content and bioaccessibility in food is critical in guiding the development of Se-enriched products. In this study, Se-enriched Pleurotus eryngii was obtained by applying different Se supplements to the substrate. Selenium content and its bioaccessibility among raw and processed fruit bodies were compared. The application of exogenous Se had no significant effect on the yield of P. eryngii, while amendment Se yeast could slightly promote the growth of P. eryngii. The enrichment ability of P. eryngii among different Se supplements declined in the order of NaSeO > Se yeast > NaSeO. However, the processing treatments resulted in 6.6%-45.9% Se loss. The Se bioaccessibility of P. eryngii was 78.4%-89.7%. Frying treatment reduced Se bioaccessibility in samples, whereas boiling treatment enhanced it. Therefore, Se yeast and boiling treatment are recommended as the ideal Se supplement and processing method for Se-enriched P. eryngii.
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http://dx.doi.org/10.1016/j.foodchem.2020.127661DOI Listing
February 2021

Effective biosorption of uranium from aqueous solution by cyanobacterium Anabaena flos-aquae.

Environ Sci Pollut Res Int 2020 Dec 6;27(35):44306-44313. Epub 2020 Aug 6.

State Key Laboratory of Nuclear Resources and Environment, East China University of Technology, Nanchang, 330013, China.

Anabaena flos-aquae, a typical species of cyanobacterial bloom, was employed as a useful biosorbent for uranium removal. Batch experiments were conducted to examine the effects of different parameters on the uranium uptake amount of Anabaena flos-aquae. The maximum adsorption capacity of 196.4 mg/g was obtained under the optimized experimental conditions. The calculations of kinetic and thermodynamic results proved the adsorption process was endothermic, chemisorption, and spontaneous. The adsorption of uranium onto Anabaena flos-aquae was better defined by the Langmuir model, which indicated the process was a monolayer sorption. In addition, the characterization of the biosorbent before and after uranium sorption implied that the dominant functional groups participated in the uranium adsorption process were hydroxyl, amino, and carboxyl. In conclusion, the environmentally friendly and biocompatible characteristics of Anabaena flos-aquae suggest that it can be a promising biosorbent for uranium removal.
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http://dx.doi.org/10.1007/s11356-020-10364-4DOI Listing
December 2020

Oestrogen induces epithelial-mesenchymal transition in endometriosis via circ_0004712/miR-148a-3p sponge function.

J Cell Mol Med 2020 09 15;24(17):9658-9666. Epub 2020 Jul 15.

Department of Reproductive Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.

Endometriosis is a common, chronic gynaecologic disease affecting up to 10% of women in their reproductive age and leading to pain and infertility. Oestrogen (E )-induced epithelial-mesenchymal transition (EMT) process has been considered as a key factor of endometriosis development. Recently, the dysregulated circular RNAs (circRNAs) have been discovered in endometriosis tissues. However, the molecular mechanism of circRNAs on the E -induced EMT process in endometriosis is still unknown. Here, we demonstrated that circ_0004712 up-regulated by E treatment in endometrial epithelial cells. Knock-down the expression of circ_0004712 significantly suppressed E -induced cell migration activity. Meanwhile, we identified miR-148a-3p as a potential target miRNA of circ_0004712. Inhibited the expression of miR-148a-3p could recovered the effect of circ_0004712 knock-down in E -treated endometrial epithelial. Furthermore, Western blot assay showed that E treatment could increase the expression and activity of β-catenin, snail and N-cadherin and reduce the expression of E-cadherin. The expression and activity of β-catenin pathway were recovered by circ_0004712 knock-down or miR-148a-3p overexpression. Altogether, the results demonstrate that circ_0004712/miR-148a-3p plays an important role in E -induced EMT process in the development of endometriosis, and the molecular mechanism may be associated with the β-catenin pathway. This work highlighted the importance of circRNAs in the development of endometriosis and provide a new biomarker for diagnosis and therapies.
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http://dx.doi.org/10.1111/jcmm.15495DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7520264PMC
September 2020

New $\text{C}_{n}^{2}$ statistical model based on first radiosonde turbulence observation over Lhasa.

J Opt Soc Am A Opt Image Sci Vis 2020 Jun;37(6):995-1001

It is worth highlighting that, for the first time to the best of our knowledge, vertical profiles of atmospheric parameters and $C_n^2$ were measured at Lhasa, south of the Tibetan Plateau, using balloon-borne radiosondes. Based on the measurements, two new statistical models (Lhasa HMN and Lhasa Dewan) for estimating turbulence strength are proposed. Attention has been paid to evaluate the reliability of the two models to reconstruct vertical profiles of $C_n^2$ from a statistical perspective. The statistical analyses presenting the Lhasa HMN model are accompanied with lower bias, root mean square error (RMSE), and bias-corrected RMSE ($\sigma$) than those of the Lhasa Dewan model, which implies the Lhasa HMN model can better reveal the nature of turbulence characteristics of Lhasa influenced by unique local weather conditions. In addition, the comparison between the Lhasa HMN model and measurements in calculating integrated astroclimatic parameters is carried out, and the result suggests that the performance of the Lhasa HMN model is reliable and satisfactory. The new reliable $C_n^2$ model offers new insight into the characteristics of optical turbulence at Lhasa and provides support for pursuing astronomical site selection in the Tibetan Plateau.
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http://dx.doi.org/10.1364/JOSAA.387211DOI Listing
June 2020

A point-to-point protein-protein interaction assay reveals the signaling interplays among plant hormones and environmental cues.

Plant Direct 2020 May 25;4(5):e00228. Epub 2020 May 25.

Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life Sciences Nanjing Normal University Nanjing China.

As sessile organisms, plants must properly coordinate their growth and developmental programs with changes in the environment. The integration of exogenous environmental cues with endogenous plant hormone responses often occurs through physical protein-protein interactions (PPIs). However, a comprehensive PPI network that mediates environmental and hormonal responses has not been established. In this study, we initially cloned 113 phytohormone-related genes and 29 light signaling components of and then individually tested their mutual interactions (in total 2,655 tests) using a yeast-two-hybrid approach to ultimately identify 141 interactions. Based on these interaction results, we next revealed the signaling cross talk between jasmonate and abscisic acid by characterizing the JAZ1-PYL4 and JAZ1-ABI1 interactions. Thus, we generated a useful resource for the community to explore the molecular mechanisms underlying signaling interactions between plant hormones and/or with light.
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http://dx.doi.org/10.1002/pld3.228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247280PMC
May 2020

Hydrogen Terminated Germanene for a Robust Self-Powered Flexible Photoelectrochemical Photodetector.

Small 2020 Jun 10;16(23):e2000283. Epub 2020 May 10.

Institute for Superconducting and Electronic Materials (ISEM), Australian Institute for Innovative Materials (AIIM), University of Wollongong, Wollongong, NSW, 2500, Australia.

As a rising star in the family of graphene analogues, germanene shows great potential for electronic and optical device applications due to its unique structure and electronic properties. It is revealed that the hydrogen terminated germanene not only maintains a high carrier mobility similar to that of germanene, but also exhibits strong light-matter interaction with a direct band gap, exhibiting great potential for photoelectronics. In this work, few-layer germanane (GeH) nanosheets with controllable thickness are successfully synthesized by a solution-based exfoliation-centrifugation route. Instead of complicated microfabrication techniques, a robust photoelectrochemical (PEC)-type photodetector, which can be extended to flexible device, is developed by simply using the GeH nanosheet film as an active electrode. The device exhibits an outstanding photocurrent density of 2.9 µA cm with zero bias potential, excellent responsivity at around 22 µA W under illumination with intensity ranging from 60 to 140 mW cm , as well as short response time (with rise and decay times, t = 0.24 s and t = 0.74 s). This efficient strategy for a constructing GeH-based PEC-type photodetector suggests a path to promising high-performance, self-powered, flexible photodetectors, and it also paves the way to a practical application of germanene.
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http://dx.doi.org/10.1002/smll.202000283DOI Listing
June 2020

The GRIA3 c.2477G > A Variant Causes an Exaggerated Startle Reflex, Chorea, and Multifocal Myoclonus.

Mov Disord 2020 07 5;35(7):1224-1232. Epub 2020 May 5.

Department of Pharmacology and Chemical Biology, Emory University School of Medicine, Atlanta, Georgia, USA.

Background: Hemizygous mutations in GRIA3 encoding the GluA3 subunit of the amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor are known to be associated with neurodevelopmental disorders, including intellectual disability, hypotonia, an autism spectrum disorder, sleep disturbances, and epilepsy in males.

Objective: To describe a new and consistent phenotype in 4 affected male patients associated with an undescribed deleterious variant in GRIA3.

Methods: We evaluated a large French family in which segregate a singular phenotype according to an apparent X-linked mode of inheritance. Molecular analyses using next generation sequencing and in vitro functional studies using 2-electrode voltage clamp recordings on Xenopus laevis oocytes and a β-lactamase reporter assay in transfected human embryonic kidney (HEK293) cells were performed.

Results: In addition to mild intellectual disability and dysarthria, affected patients presented a tightly consistent early-onset movement disorder combining an exaggerated startle reflex with generalized chorea and multifocal myoclonus. The unreported GRIA3 missense variant c.2477G > A; p.(Gly826Asp) affecting the fourth transmembrane domain of the protein was identified in index patients and their unaffected mothers. Functional studies revealed that variant receptors show decreased current response evoked by agonist (ie, kainic acid and glutamate) and reduced expression on the cell surface in favor of pathogenicity by a loss-of-function mechanism.

Conclusions: Taken together, our results suggest that apart from known GRIA3-related disorders, an undescribed mutation-specific singular movement disorder does exist. We thus advocate considering GRIA3 mutations in the differential diagnosis of hyperekplexia and generalized chorea with myoclonus. © 2020 International Parkinson and Movement Disorder Society.
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http://dx.doi.org/10.1002/mds.28058DOI Listing
July 2020

Endosidin20 Targets the Cellulose Synthase Catalytic Domain to Inhibit Cellulose Biosynthesis.

Plant Cell 2020 07 23;32(7):2141-2157. Epub 2020 Apr 23.

Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907

Plant cellulose is synthesized by rosette-structured cellulose synthase (CESA) complexes (CSCs). Each CSC is composed of multiple subunits of CESAs representing three different isoforms. Individual CESA proteins contain conserved catalytic domains for catalyzing cellulose synthesis, other domains such as plant-conserved sequences, and class-specific regions that are thought to facilitate complex assembly and CSC trafficking. Because of the current lack of atomic-resolution structures for plant CSCs or CESAs, the molecular mechanism through which CESA catalyzes cellulose synthesis and whether its catalytic activity influences efficient CSC transport at the subcellular level remain unknown. Here, by performing chemical genetic analyses, biochemical assays, structural modeling, and molecular docking, we demonstrate that Endosidin20 (ES20) targets the catalytic site of CESA6 in Arabidopsis (). Chemical genetic analysis revealed important amino acids that potentially participate in the catalytic activity of plant CESA6, in addition to previously identified conserved motifs across kingdoms. Using high spatiotemporal resolution live cell imaging, we found that inhibiting the catalytic activity of CESA6 by ES20 treatment reduced the efficiency of CSC transport to the plasma membrane. Our results demonstrate that ES20 is a chemical inhibitor of CESA activity and trafficking that represents a powerful tool for studying cellulose synthesis in plants.
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http://dx.doi.org/10.1105/tpc.20.00202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346566PMC
July 2020

Preparation, Characterization of Propargyl Terminal Polybutadiene and Its Crosslinked Elastomers Properties.

Polymers (Basel) 2020 Mar 30;12(4). Epub 2020 Mar 30.

School of Materials, Beijing Institute of Technology, Beijing 100081, China.

Propargyl terminal Polybutadiene (PTPB) was successfully prepared through hydroxyl terminal polybutadiene (HTPB) end-capping modification. The FTIR and C NMR results indicated that the HTPB terminal hydroxyl was thoroughly replaced and yielded the target product, PTPB, with a theoretical propargyl content of 0.66 mmol g. In comparison with HTPB, PTPB has a lower viscosity. Using 1,6-diazide hexane as a curing agent, polytriazole crosslinked polybutadiene (PTPB) elastomers with various functional molar ratios () were prepared by CuAAC reaction, and the glass transition temperatures of the resultant PTPB elastomers were approximately -75 °C, measured by differential scanning calorimetry (DSC), nearly independent of elastomer values. Mechanical tests indicated, that with the increase in , the mechanical properties of PTPB elastomers exhibit a parabolic dependence on . In addition, the thermal stability of PTPB elastomers were also studied. The findings revealed some fundamental features of polytriazole crosslinking elastomer prepared by CuAAC reaction.
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http://dx.doi.org/10.3390/polym12040748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240571PMC
March 2020

The iTRAQ-based chloroplast proteomic analysis of Triticum aestivum L. leaves subjected to drought stress and 5-aminolevulinic acid alleviation reveals several proteins involved in the protection of photosynthesis.

BMC Plant Biol 2020 Mar 4;20(1):96. Epub 2020 Mar 4.

College of Food Science and Technology, Henan Agricultural University, No. 63, Nongye Rd., Zhengzhou, 450002, Henan Province, China.

Backgrounds: The perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. The exogenous application of 5-aminolevulinic acid (ALA) mitigates the damage caused by drought stress, protecting plant growth and development, but the regulatory mechanism behind this process remains obscure.

Results: Wheat seedlings were drought treated, and the iTRAQ-based proteomic approach was employed to assess the difference in chloroplast protein content caused by exogenous ALA. A total of 9499 peptides, which could be classified into 2442 protein groups, were identified with ≤0.01 FDR. Moreover, the contents of 87 chloroplast proteins was changed by drought stress alone compared to that of the drought-free control, while the contents of 469 was changed by exogenous ALA application under drought stress compared to that of drought stress alone. The Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results suggested that the ALA pretreatment adjusted some biological pathways, such as metabolic pathways and pathways involved in photosynthesis and ribosomes, to enhance the drought resistance of chloroplasts. Furthermore, the drought-promoted HO accumulation and O production in chloroplasts were alleviated by the exogenous pretreatment of ALA, while peroxidase (POD) and glutathione peroxidase (GPX) activities were upregulated, which agreed with the chloroplast proteomic data. We suggested that ALA promoted reactive oxygen species (ROS) scavenging in chloroplasts by regulating enzymatic processes.

Conclusions: Our results from chloroplast proteomics extend the understanding of the mechanisms employed by exogenous ALA to defend against drought stress in wheat.
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http://dx.doi.org/10.1186/s12870-020-2297-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7057492PMC
March 2020

The effects of Aeromonas hydrophila infection on oxidative stress, nonspecific immunity, autophagy, and apoptosis in the common carp.

Dev Comp Immunol 2020 04 23;105:103587. Epub 2019 Dec 23.

College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China. Electronic address:

Although the toxicity of Aeromonas hydrophila infection to common carp has been characterized, the mechanisms underlying this toxicity have not been fully explored. The present study assessed the effects of A. hydrophila infection on oxidative stress, nonspecific immunity, autophagy, and apoptosis in the common carp (Cyprinus carpio). We measured the effects of 7.55 × 10 CFU/mL and 4.87 × 10 CFU/mL A. hydrophila on carp after 1, 3, 5, and 7 d of infection. GSH and SOD activity levels in the serum, liver, intestine, and gills generally increased during the early stage of infection, but significantly decreased (P < 0.05) on the seventh day. In addition, MDA levels were significantly increased throughout the infection period. The activity levels of ACP, AKP, and LZM in the liver and intestine increased on the first day after infection, then decreased on the fifth and seventh days. The mRNA expressions levels of the autophagy-associated genes atg12, atg5, LC3-II, and BECN1 in the liver, kidney, and brain substantially increased on the third day after infection (P < 0.05), while mTOR was significantly downregulated on the first and third days (P < 0.05). Western blot analysis indicated that the ratio of LC3B-ǁ/LC3B-ǀ significantly increased (P < 0.05) on days 3 and 5 post infection. Furthermore, the apoptosis-related gene Bcl-2 was significantly (P < 0.05) upregulated in the liver, kidney, and brain of the treatment group on the first and third days, while caspase3 was significantly downregulated (P < 0.05). In conclusion, our results demonstrate that A. hydrophila infection causes oxidative stress, stimulates nonspecific immune reactions, and results in autophagy in the common carp, possibly initiating apoptosis in the late stage of infection. The results of this study provide new insights into the mechanism of A. hydrophila infection in carp.
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http://dx.doi.org/10.1016/j.dci.2019.103587DOI Listing
April 2020

Contrasting Biogeographic Patterns of Bacterial and Archaeal Diversity in the Top- and Subsoils of Temperate Grasslands.

mSystems 2019 Oct 1;4(5). Epub 2019 Oct 1.

Institute of Ecology and Key Laboratory for Earth Surface Processes of the Ministry of Education, College of Urban and Environmental Sciences, Peking University, Beijing, China

Biogeographic patterns and drivers of soil microbial diversity have been extensively studied in the past few decades. However, most research has focused on the topsoil, while the subsoil is assumed to have microbial diversity patterns similar to those of the topsoil. Here we compared patterns and drivers of microbial alpha and beta diversity in and between topsoils (0 to 10 cm) and subsoils (30 to 50 cm) of temperate grasslands in Inner Mongolia of China, covering an ∼1,500-km transect along an aridity gradient. Counter to the conventional assumption, we find contrasting biogeographic patterns of diversity and influencing factors for different bacterial and archaeal groups and between depths. While bacterial diversity remains constant or increases with increasing aridity in topsoil and decreases in subsoil, archaeal diversity decreases in topsoil and remains constant in subsoil. Microbial diversity in the topsoil is most strongly influenced by aboveground vegetation and contemporary climate but is most strongly influenced by the factor historical temperature anomaly since the Last Glacial Maximum (LGM) and by soil pH in the subsoil. Moreover, the biogeographic patterns of topsoil-subsoil community dissimilarities vary for different microbial groups and are overall most strongly influenced by soil fertility differences between depths for bacteria and by contemporary climate for archaea. These findings suggest that diversity patterns observed in the topsoil may not be readily applied to the subsoil horizons. For the subsoil in particular, historical climate plays a vital role in the spatial variation of bacterial diversity. Overall, our study provides novel information for understanding and predicting soil microbial diversity patterns at depth. Exploring the biogeographic patterns of soil microbial diversity is critical for understanding mechanisms underlying the response of soil processes to climate change. Using top- and subsoils from an ∼1,500-km temperate grassland transect, we find divergent patterns of microbial diversity and its determinants in the topsoil versus the subsoil. Furthermore, we find important and direct legacy effects of historical climate change on the microbial diversity of subsoil yet indirect effects on topsoil. Our findings challenge the conventional assumption of similar geographic patterns of soil microbial diversity along soil profiles and help to improve our understanding of how soil microbial communities may respond to future climate change in different regions with various climate histories.
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http://dx.doi.org/10.1128/mSystems.00566-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6774019PMC
October 2019

Soil respiration from fields under three crop rotation treatments and three straw retention treatments.

PLoS One 2019 23;14(9):e0219253. Epub 2019 Sep 23.

College of Agronomy, Northwest A & F University, Yangling, Shaanxi, China.

Straw retention is an effective method to conserve soil water content and improve soil carbon stocks. However, how soil carbon dynamics respond to different straw retention practices remains unclear. In this study, we investigated soil respiration and soil carbon sequestration at depths of 0-100 cm. We conducted a two-year field experiment with three crop rotation treatments and three straw retention treatments in northwest China. The straw retention treatments included no straw retention (NS), retention of half the straw (HS), and retention of the total amount of straw (TS). The crop rotations treatments included winter wheat plus summer soybean (WS), winter wheat plus summer maize (WM), and winter wheat plus summer fallow (WF). Mean soil respiration rates under WS, WM, and WF treatments were 5.14, 6.53, and 5.49 μmol·m-2·s-1; and 5.67, 5.47, and 6.03 μmol·m-2·s-1 under TS, HS, and NS treatments. The mean soil water content were 15.50%, 15.57%, and 15.74% under WS, WM, and WF rotations, and 15.81%, 15.41%, and 15.50% under TS, HS, and NS treatments. The soil organic carbon (SOC) concentration was higher with increased straw retention, and lower at deeper soil depths. Mean SOC concentrations under different rotations and straw treatments of TS, HS, and NS, respectively were as follows: WS: 6.91, 6.63, 6.39 g/kg; WM: 6.90, 6.72, 6.57 g/kg; and WF: 6.49, 6.52, 6.37 g/kg. Soil temperature was the main determinant of soil respiration rates. We conclude that WS rotation resulted in lower soil respiration, WM rotation resulted in a higher soil carbon sequestration potential, and WF rotation resulted in higher soil water content. However, continued, long-term monitoring is needed to confirm the effect of rotations and straw retention on soil respiration and carbon sequestration in dryland cropping systems in northern China.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219253PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756547PMC
March 2020

The cotton GhWIN2 gene activates the cuticle biosynthesis pathway and influences the salicylic and jasmonic acid biosynthesis pathways.

BMC Plant Biol 2019 Aug 28;19(1):379. Epub 2019 Aug 28.

College of Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.

Background: Metabolic pathways are interconnected and yet relatively independent. Genes involved in metabolic modules are required for the modules to run. Study of the relationships between genes and metabolic modules improves the understanding of metabolic pathways in plants. The WIN transcription factor activates the cuticle biosynthesis pathway and promotes cuticle biosynthesis. The relationship between the WIN transcription factor and other metabolic pathways is unknown. Our aim was to determine the relationships between the main genes involved in cuticle biosynthesis and those involved in other metabolic pathways. We did this by cloning a cotton WIN gene, GhWIN2, and studying its influence on other pathways.

Results: As with other WIN genes, GhWIN2 regulated expression of cuticle biosynthesis-related genes, and promoted cuticle formation. Silencing of GhWIN2 resulted in enhanced resistance to Verticillium dahliae, caused by increased content of salicylic acid (SA). Moreover, silencing of GhWIN2 suppressed expression of jasmonic acid (JA) biosynthesis-related genes and content. GhWIN2 positively regulated the fatty acid biosynthesis pathway upstream of the JA biosynthesis pathway. Silencing of GhWIN2 reduced the content of stearic acid, a JA biosynthesis precursor.

Conclusions: GhWIN2 not only regulated the cuticle biosynthesis pathway, but also positively influenced JA biosynthesis and negatively influenced SA biosynthesis.
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http://dx.doi.org/10.1186/s12870-019-1888-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6712776PMC
August 2019

Spatial Effects of Environmental Regulation and Green Credits on Green Technology Innovation Under Low-Carbon Economy Background Conditions.

Int J Environ Res Public Health 2019 08 21;16(17). Epub 2019 Aug 21.

Research Center for Smarter Supply Chain, Dongwu Business School, Soochow University, Suzhou 215000, China.

Based on the data of green credit (GC), environmental regulation (ER) and green technology innovation (GTI) in 30 provinces and cities of China from 2007 to 2016, this study investigated the relationship between green credit and green technology innovation development and analyzed the adjustment effect of ER on GC to promote GTI using Geoda and Matlab2016 software, so as to further guide and encourage GC. The results show that GTI in 30 provinces and municipalities in China has a significant spatial agglomeration effect. Single GC plays a certain role in promoting local technology innovation, but it fails to influences the surrounding areas. Environmental regulation has a certain regulatory effect on the relationship between green credit and green technology innovation in the province but also fails to influences the surrounding areas.
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http://dx.doi.org/10.3390/ijerph16173027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747161PMC
August 2019

Physiological responses of a coccolithophore to multiple environmental drivers.

Mar Pollut Bull 2019 Sep 17;146:225-235. Epub 2019 Jun 17.

State Key Laboratory of Marine Environmental Science (Xiamen University)/College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address:

Ocean acidification is known to affect primary producers differentially in terms of species and environmental conditions, with controversial results obtained under different experimental setups. In this work we examined the physiological performances of the coccolithophore Gephyrocapsa oceanica that had been acclimated to 1000 μatm CO for ~400 generations, and then exposed to multiple drivers, light intensity, light fluctuating frequency, temperature and UV radiation. Here, we show that increasing light intensity resulted in higher non-photochemical quenching and the effective absorption cross-section of PSII. The effective photochemical efficiency (Fv'/Fm') decreased with increased levels of light, which was counterbalanced by fluctuating light regimes. The greenhouse condition acts synergistically with decreasing fluctuating light frequency to increase the Fv'/Fm' and photosynthetic carbon fixation rate. Our data suggest that the coccolithophorid would be more stressed with increased exposures to solar UV irradiances, though its photosynthetic carbon fixation could be enhanced under the greenhouse condition.
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http://dx.doi.org/10.1016/j.marpolbul.2019.06.032DOI Listing
September 2019

Clinical implications of sarcomere and nonsarcomere gene variants in patients with left ventricular noncompaction cardiomyopathy.

Mol Genet Genomic Med 2019 09 9;7(9):e874. Epub 2019 Aug 9.

Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Robust data regarding genotype-phenotype correlations in left ventricular noncompaction cardiomyopathy (LVNC) are lacking.

Methods: About 72 cardiomyopathy-related genes were comprehensively screened in a cohort of LVNC patients using targeted sequencing. Baseline and follow-up data were collected. The primary endpoint was a composite of death and heart transplantation.

Results: A total of 83 unrelated adult patients were included in analyses. Following stringent classification according to the American College of Medical Genetics and Genomics (ACMG) guidelines, 36 pathogenic variants of 14 genes were detected in 32 patients. Among them, 12 patients carried at least one nonsarcomere variant (NSV). At baseline, NSV carriers had a higher frequency of atrial fibrillation, but lower left ventricular ejection fraction, than did noncarriers. During a median follow-up of 4.2 years, NSV carriers experienced a higher rate of the primary endpoint compared with noncarriers. There was no significant difference in the rate between carriers of sarcomere variant (SV) and noncarriers, as well as between carriers of SV and NSV. The presence of NSV was associated with an increased risk of the primary endpoint independent of age, sex, and cardiac function (hazard ratio: 3.61, 95% confidence interval: 1.42-9.19, p = .002).

Conclusion: NSV may act as a genetic modifier and worsen the clinical phenotype in patients with LVNC.
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http://dx.doi.org/10.1002/mgg3.874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732340PMC
September 2019

MicroRNA-153 improves the neurogenesis of neural stem cells and enhances the cognitive ability of aged mice through the notch signaling pathway.

Cell Death Differ 2020 02 11;27(2):808-825. Epub 2019 Jul 11.

Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092, China.

Aging-related cognitive ability impairments are one of the main threats to public health, and impaired hippocampal neurogenesis is a major cause of cognitive decline during aging. However, the regulation of adult neurogenesis in the hippocampus requires further study. Here, we investigated the role of microRNA-153 (miR-153), a highly conserved microRNA in mice and humans, in adult neurogenesis. During the passaging of neural stem cells (NSCs) in vitro, endogenous miR-153 expression was downregulated, with a decrease in neuronal differentiation ability. In addition, miR-153 overexpression increased the neurogenesis of NSCs. Further studies showed that miR-153 regulated neurogenesis by precisely targeting the Notch signaling pathway through inhibition of Jagged1 and Hey2 translation. In vivo analysis demonstrated that miR-153 expression was decreased in the hippocampi of aged mice with impaired cognitive ability, and that miR-153 overexpression in the hippocampus promoted neurogenesis and markedly increased the cognitive abilities of the aged mice. Overall, our findings revealed that miR-153 affected neurogenesis by regulating the Notch signaling pathway and elucidated the function of miR-153 in aging-related, hippocampus-dependent cognitive ability impairments, and neurodegenerative diseases.
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http://dx.doi.org/10.1038/s41418-019-0388-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206122PMC
February 2020

GhABP19, a Novel Germin-Like Protein From , Plays an Important Role in the Regulation of Resistance to Verticillium and Fusarium Wilt Pathogens.

Front Plant Sci 2019 8;10:583. Epub 2019 May 8.

College of Science, China Agricultural University, Beijing, China.

Germin-like proteins (GLPs) are water-soluble plant glycoproteins belonging to the cupin superfamily. The important role of GLPs in plant responses against various abiotic and biotic stresses, especially pathogens, is well validated. However, little is known about cotton GLPs in relation to fungal pathogens. Here, a novel GLP gene was isolated from and designated as . The expression of was upregulated in cotton plants inoculated with and and in response to treatment with jasmonic acid (JA) but was suppressed in response to salicylic acid treatment. A relatively small transient increase in was seen in HO treated samples. The three-dimensional structure prediction of the GhABP19 protein indicated that the protein has three histidine and one glutamate residues responsible for metal ion binding and superoxide dismutase (SOD) activity. Purified recombinant GhABP19 exhibits SOD activity and could inhibit growth of , , , , and . To further verify the role of in fungal resistance, -overexpressing plants and -silenced cotton plants were developed. GhABP19-transgenic lines showed much stronger resistance to and infection than control (empty vector) plants did. On the contrary, silencing of in cotton conferred enhanced susceptibility to fungal pathogens, which resulted in necrosis and wilt on leaves and vascular discoloration in -silenced cotton plants. The HO content and endogenous SOD activity were affected by expression levels in and cotton plants after inoculation with and , respectively. Furthermore, overexpression or silencing resulted in activation or suppression of JA-mediated signaling, respectively. Thus, GhABP19 plays important roles in the regulation of resistance to verticillium and fusarium wilt in plants. These modulatory roles were exerted by its SOD activity and ability to activate the JA pathway. All results suggest that was involved in plant disease resistance.
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http://dx.doi.org/10.3389/fpls.2019.00583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6517559PMC
May 2019

Enhanced resistance to Verticillium dahliae mediated by an F-box protein GhACIF1 from Gossypium hirsutum.

Plant Sci 2019 Jul 17;284:127-134. Epub 2019 Apr 17.

College of Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China. Electronic address:

Avr9/Cf-9-INDUCED F-BOX1 (ACIF1) was first identified during screening of Avr9/Cf-9-elicited genes in tobacco. Further analysis revealed that ACIF1 was required for hypersensitive responses triggered by various elicitors in tobacco and tomato, indicating that it may be involved in various disease resistance. Here, we cloned its cotton (Gossypium hirsutum) homolog GhACIF1, which encodes an F-box protein. We show that GhACIF1 interacts with the putative SKP1-like protein, named GhSKP1. Disease resistance assays show that GhACIF1 enhances resistance to Verticillium dahliae in Arabidopsis plants, while silencing of GhACIF1 confers sensitivity to V. dahliae in cotton. Further analysis show that PevD1 elicitor activates hypersensitive and acquired immune response mediated by GhACIF1. Collectively, these results indicate that GhACIF1 contributes to protection against V. dahliae infection.
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http://dx.doi.org/10.1016/j.plantsci.2019.04.013DOI Listing
July 2019