Publications by authors named "Nan Zhang"

2,953 Publications

  • Page 1 of 1

root-rot associated shifts in microbiomes of root endosphere, rhizosphere, and soil.

PeerJ 2022 4;10:e13808. Epub 2022 Aug 4.

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, China.

Root-rot disease has lead to serious reduction in yields and jeopardized the survival of the economically and ecologically important trees cultured in Sichuan Province. In order to investigate the interaction between the microbiome and the root-rot disease, a metagenomic analysis was performed to characterize the microbial communities and functions in root endosphere, rhizosphere and bulk soil with/without root-rot disease. Soil physicochemical properties, microbial population size and enzyme activities were also analyzed for finding their interactions with the root-rot disease. As results, lower total nitrogen (TN) and available phosphorus (AP) contents but higher pH in rhizosphere and bulk soil, as well as lower substrate-induced respiration (SIR) and higher protease activity in bulk soil of diseased trees were found, in comparison with that of healthy trees. Microbial diversity and community composition were changed by root-rot disease in the endosphere, but not in rhizosphere and bulk soils. The endophytic microbiome of diseased trees presented higher Proteobacteria abundance and lower abundances of Bacteroidetes, Firmicutes and dominant fungal phyla. The relative abundances of nitrogen cycle- and carbon cycle-related genes in endophytic microbiomes were different between the diseased and healthy trees. Based on ANOSIM and PCoA, functional profiles (KEGG and CAZy) of microbiomes in rhizosphere and bulk soil shifted significantly between the diseased and healthy trees. In addition, soil pH, TN, AP, SIR, invertase and protease were estimated as the main factors influencing the shifts of taxonomic and functional groups in microbiomes of rhizosphere and bulk soil. Conclusively, the imbalance of root and soil microbial function groups might lead to shifts in the root endosphere-rhizosphere microenvironment, which in turn resulted in root-rot.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7717/peerj.13808DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357373PMC
August 2022

Gender-Specific Association Between Nighttime Sleep Duration and Falls Among Community-Dwelling Middle-Aged and Older Adults in China.

J Appl Gerontol 2022 Aug 9:7334648221118904. Epub 2022 Aug 9.

School of Nursing, 74790Xinjiang Medical University, Urumqi, China.

This study was conducted using the data from 2015 China Health and Retirement Longitudinal Study (CHARLS). Gender-specific association between nighttime sleep duration (NSD) and falls were evaluated through multiple logistic regression by adjusting for confounding variables. Of the 22,899 respondents, 18,446 were eligible and 3,144 (16.99%) had more than a single fall. Short and long NSD were associated with high occurrences of falls in the total and female population, but only a short NSD was associated with a high occurrence of falls in the male population. In conclusion, NSD was strongly and significantly associated with falls, but the association was gender-specific. Accordingly, increasing targeted, evidence-based and gender-specific preventions for NSD and sleep disturbance are necessary among primary care interventions to prevent falls.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/07334648221118904DOI Listing
August 2022

Synergetic Insulation and Induction Effects Selectively Optimize Multiresonance Thermally Activated Delayed Fluorescence.

Research (Wash D C) 2022 2;2022:9838120. Epub 2022 Jun 2.

Key Laboratory of Functional Inorganic Material Chemistry, Ministry of Education, School of Chemistry and Materials, Heilongjiang University, China.

Multiresonance (MR) emitters featuring narrowband emissions and theoretically 100% exciton harvesting are great potential for organic light-emitting diode (OLED) applications. However, how to functionalize MR molecules without scarifying emission color purity is still a key challenge. Herein, we report a feasible strategy for selective optimization of MR molecules, which is demonstrated by a blue MR emitter substituted with a diphenylphosphine oxide (DPPO) group. Compared to its DPPO-free parent molecule, preserves narrowband feature with full widths at half maximum (FWHM) values of 28 nm in film and 32 nm in OLEDs and achieves 40% increased photoluminescence (92%) and electroluminescence quantum efficiencies (28%). It is showed that insulation effect of P=O effectively confines the singlet excited state on MR core to keep emission color purity, and its induction effect enhances singlet radiation and triplet-to-singlet conversion. This synergism for selective optimization is based on rational linkage between MR core and functional groups.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.34133/2022/9838120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275084PMC
June 2022

Integrative analysis of the expression and prognosis for CENPs in ovarian cancer.

Genomics 2022 Aug 4:110445. Epub 2022 Aug 4.

Department of Obstetrics and Gynecology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, China; Department of Gynecology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, China. Electronic address:

Centromere proteins (CENPs) are nuclear proteins that are involved in centromere formation and chromosome segregation during mitosis. Some members of CENPs have been extensively studied in the initiation and development of cancers. However, the expression patterns and exact roles of CENPs in ovarian cancer (OC) have not been fully elucidated. In this study, we comprehensively assessed the genetic variation, expression patterns and prognostic value of CENPs in OC by several databases. The mRNA expression levels of CENPA/F/H/L/N/U/W were found to be significantly upregulated in OC and related to worse prognosis. Additionally, function enrichment analysis showed that CENPs were involved in DNA repair and cell division. Meanwhile, immune infiltration analysis elucidated that CENPs were associated with myeloid-derived suppressor cells (MDSCs) and major histocompatibility complex (MHC). These results suggested that CENPs might serve as potential diagnostic and prognostic markers and provide new insights for the development of CENPs-targeted therapeutics for ovarian cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygeno.2022.110445DOI Listing
August 2022

Association between leucocyte telomere length and the risk of atrial fibrillation: An updated systematic review and meta-analysis.

Ageing Res Rev 2022 Aug 3;81:101707. Epub 2022 Aug 3.

Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China. Electronic address:

Background And Aims: Advancing age is the most important risk factor of atrial fibrillation (AF). The shortening of telomere length is a biomarker of biologic aging. There is an increasing body of evidence that leucocyte telomere length (LTL) is associated with the risk of AF development. However, the results in these studies were controversial. The current systematic review and meta-analysis was conducted to examine the role of LTL in predicting the incidence of AF.

Methods And Results: Observational studies reporting the association between LTL and the risk of AF were retrieved through 25th June, 2022 from PubMed and Embase. A total of twelve studies including 18,293 patients were included in the present analysis. Leucocyte telomere shortening was found to be an independent predictor of AF as a continuous variable in both univariate [OR:2.14; 95%CI(1.48,3.10); P < 0.0001] and multivariate analyses [OR:1.41;95%CI(1.11,1.79); P = 0.005], as well as categorical variable in multivariate analysis [OR:1.53; 95%CI(1.04,2.27); P = 0.03]. Furthermore, leucocyte telomere shortening was significantly associated with recurrent AF [OR:4.32;95%CI(2.42,7.69); P < 0.00001] but not new-onset AF [OR:1.14; 95%CI(0.90,1.45); P = 0.29]. Leucocyte telomere shortening was also associated with an increased risk of persistent AF [OR:14.73;95%CI (3.16,68.67); P = 0.0006] and paroxysmal AF [OR:2.74;95%CI(1.45,5.18); P = 0.002]. Besides, LTL was an independent predictor for progression from paroxysmal AF to persistent AF [OR:3.2;95%CI(1.66,6.18); P = 0.0005]. Differences between males [OR:1.99; 95%CI(1.29,3.06); P = 0.002] and females [OR:0.86; 95%CI (0.29,2.56);P = 0.79] were observed.

Conclusions: Leucocyte telomere shortening predicts the risk of AF, especially recurrent AF. The predictive value is more prominent in males than in females. Shortening in LTL can predict the progression from paroxysmal to persistent AF.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.arr.2022.101707DOI Listing
August 2022

A 5-year retrospective analysis of the risk factors, treatment and prognosis of patients with fungal keratitis in Heilongjiang.

Am J Ophthalmol 2022 Aug 3. Epub 2022 Aug 3.

Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Basic and Clinical Research of Heilongjiang Province, Harbin, Heilongjiang Province, People's Republic of China. Electronic address:

Purpose: To report the epidemiological features, laboratory findings, and treatment outcomes of patients with fungal keratitis (FK) during a busy farming period (May, June, September, October and November) in Heilongjiang Province, China.

Design: Retrospective, observational case series.

Methods: In total, 251 patients diagnosed with FK at the Eye Hospital, First Affiliated Hospital of Harbin Medical University (HMU), from 2017 to 2021 were identified. Medical records were retrospectively analyzed, and demographic features, risk factors, monthly distributions, clinical characteristics, laboratory findings, treatment strategies and prognostic data were collected.

Results: The number of FK cases in the busy farming period accounted for 74.1% (186/251) of the total. The mean patient age was 58.2±9.5 years. Males (66.7%) were more likely to develop FK than females (33.3%), and plant-related trauma was the main cause in 80.1% (149/186) of the cases. The most common causative fungal species was Fusarium (34.9%). In vivo confocal microscopy (IVCM) had the highest positivity rate for FK diagnosis (94.6%). The depth of hypopyon, depth of hyphae or spores and infiltrate width (IW) were significantly positively correlated with delayed presentation.

Conclusions: Patients with a longer time of presentation to the hospital were more likely to undergo surgery due to topical medical therapy failure. The most important measures for FK prevention are the use of eye protection during outdoor work and visiting the hospital for treatment as soon as possible after eye injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ajo.2022.07.023DOI Listing
August 2022

Directional Manipulation of Electron Transfer by Energy Level Engineering for Efficient Cathodic Oxygen Reduction.

Nano Lett 2022 Aug 5. Epub 2022 Aug 5.

School of Chemistry and Materials Science, Collaborative Innovation Center of Chemistry for Energy Materials, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China.

Electron transfer plays an important role in determining the energy conversion efficiency of energy devices. Nitrogen-coordinated single metal sites (M-N) materials as electrocatalysts have exhibited great potential in devices. However, there are still great difficulties in how to directionally manipulate electron transfer in M-N catalysts for higher efficiency. Herein, we demonstrated the mechanism of electron transfer being affected by energy level structure based on classical iron phthalocyanine (FePc) molecule/carbon models and proposed an energy level engineering strategy to manipulate electron transfer, preparing high-performance ORR catalysts. Engineering molecular energy level via modulating FePc molecular structure with nitro induces a strong interfacial electronic coupling and efficient charge transfer from carbon to FePc-β-NO molecule. Consequently, the assembled zinc-air battery exhibits ultrahigh performance which is superior to most of M-N catalysts. Energy level engineering provides a universal approach for directionally manipulating electron transfer, bringing a new concept to design efficient and stable M-N electrocatalyst.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.2c01933DOI Listing
August 2022

Biodegradation of butachlor in rice intensified by a regulator of OsGT1.

Ecotoxicol Environ Saf 2022 Aug 1;242:113942. Epub 2022 Aug 1.

Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

The constant utilization of herbicide butachlor to prevent weeds in agronomic management is leading to its growing accumulation in environment and adverse impact on crop production and food security. Some technologies proposed for butachlor degradation in waters and farmland soils are available, but the catabolic mechanism in crops polluted with butachlor remains unknown. How plants cope with the ecotoxicity of butachlor is not only a fundamental scientific question but is also of critical importance for safe crop production and human health. This study developed a genetically improved rice genotype by overexpressing a novel glycosyltransferase gene named OsGT1 to accelerate removal of butachlor residues in rice crop and its growth environment. Both transcriptional expression and protein activates of OsGT1 are considerably induced under butachlor stress. The growth of the OsGT1 overexpression rice (OsOE) was significantly improved and butachlor-induced cellular damage was greatly attenuated compared to its wild-type (WT). The butachlor concentrations in shoots and roots of the hydroponically grown OsOE plants were reduced by 14.1-30.7 % and 37.8-47.7 %. In particular, the concentrations in the grain of OsOE lines were reduced to 54.6-85.6 % of those in wild-type. Using LC-Q-TOF-HRMS/MS, twenty-three butachlor derivatives including 16 metabolites and 7 conjugations with metabolic pathways were characterized, and it turns out that the OsOE lines accumulated more degradative products than wild-type, implying that more butachlor molecules were intensively catabolized. Taken together, the reduced residues of parent butachlor in rice and its growth media point out that OsGT1 plays a critical role in detoxifying and catabolizing the poisoning chemical in plants and its environment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ecoenv.2022.113942DOI Listing
August 2022

A Nurse-Led mHealth Self-Management Program (mChemotherapy) for Breast Cancer Patients Undergoing Chemotherapy: Study Protocol of a Randomized Controlled Pilot Study.

Cancer Control 2022 Jan-Dec;29:10732748221115469

Ruijin Hospital, 66281Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: The global pandemic has caused breast cancer (BC) patients who are receiving chemotherapy to face more challenges in taking care of themselves than usual. A novel nurse-led mHealth program (mChemotherapy) is designed to foster self-management for this population. The aim of the pilot study is to determine the feasibility, usability, and acceptability of an mChemotherapy program for breast cancer patients undergoing chemotherapy. The objective also is to evaluate the preliminary effects of this program on adherence to app usage, self-efficacy, quality of life, symptom burden, and healthcare utilization among this group of patients.

Methods: This is a single-blinded randomized controlled pilot study that includes one intervention group (mChemotherapy group) and one control group (routine care group). Ninety-four breast cancer patients who commence chemotherapy in a university-affiliated hospital will be recruited. Based on the Individual and Family Self-management Theory, this 6-week mChemotherapy program, which includes a combination of self-regulation activities and nurse-led support, will be provided. Data collection will be conducted at baseline, week 3 (T1), and week 6 (T2). A general linear model will be utilized for identifying the between-group, within-group, and interaction effects. Qualitative content analysis will be adopted to analyze, extract, and categorize the interview transcripts.

Discussions: Breast cancer patients receiving chemotherapy are a population that often experiences a heavy symptom burden. During the pandemic, they have had difficulties in self-managing the side effects of chemotherapy due to the lack of face-to-face professional support. An mChemotherapy program will be adopted through a self-regulation process and with the provision of nurse-led real-time professional support for these patients. If proven effective, BC patients who engage in this program will be more likely to take an active role in managing their symptoms, take responsibility for their own health, and subsequently improve their self-efficacy and adherence to the use of the app.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/10732748221115469DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358201PMC
August 2022

Exosomes derived from myeloid-derived suppressor cells facilitate castration-resistant prostate cancer progression via S100A9/circMID1/miR-506-3p/MID1.

J Transl Med 2022 08 2;20(1):346. Epub 2022 Aug 2.

Department of Urology, Hangzhou Hospital of Traditional Chinese Medicine, 453# Tiyuchang Road, Hangzhou, 310007, Zhejiang, China.

Background: Castration-resistant prostate cancer (CRPC) is a major cause of recurrence and mortality among prostate cancer (PCa) patients. Myeloid-derived suppressor cells (MDSCs) regulate castration resistance in PCa. Previously, it was shown that intercellular communication was efficiently mediated by exosomes (Exos), but the role and the mechanism of MDSC-derived Exos in CRPC progression was unclear.

Methods: In this study, the circRNA expression profiles in PC3 cells treated with MDSC-Exo and control cells were investigated using a circRNA microarray.

Results: The data showed that circMID1 (hsa_circ_0007718) expression was elevated in PC3 cells treated with MDSC-Exo. Moreover, high circMID1 expression was found in PCa compared with benign prostatic hyperplasia (BPH) tissues and in CRPC patients compared with hormone sensitive prostate cancer (HSPC) patients. Further studies showed that MDSC-Exo accelerated PCa cell proliferation, migration, and invasion, while circMID1 deficiency inhibited MDSC-Exo-regulated CRPC progression in vitro and in vivo. Mechanistically, MDSC-derived exosomal S100A9 increased circMID1 expression to sponge miR-506-3p, leading to increased MID1 expression and accelerated tumor progression.

Conclusion: Together, our results showed that a S100A9/circMID1/miR-506-3p/MID1 axis existed in MDSC-Exo-regulated CRPC progression, which provided novel insights into MDSC-Exo regulatory mechanisms in CRPC progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12967-022-03494-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9344715PMC
August 2022

Nano-drug delivery system with enhanced tumour penetration and layered anti-tumour efficacy.

Nanomedicine 2022 Jul 27;45:102592. Epub 2022 Jul 27.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China. Electronic address:

The low delivery efficiency of nano-drugs and limited tumour penetration are still huge challenges in treating solid tumours. Herein, we developed a pH-responsive nano-drug delivery system, CALS/[email protected], with a size conversion-layered delivery function. The system is composed of a pH-responsive cationic liposome loaded with DOX (CALS) and a polyamidoamine dendrimer loaded with DOX ([email protected]) modified with 2,3-dimethylmaleic anhydride ([email protected]) using electrostatic adsorption. In the tumour microenvironment, the positively-charged large-size CALS and the positively-charged small-size [email protected] were dissociated to exert anti-tumour effects. CALS preferentially targeted tumour angiogenesis endothelial cells. Because of its small size and positive charge, [email protected] showed excellent tumour penetration. Significant tumour suppression by the system in vivo was confirmed in a 4T1 tumour xenograft mouse model. This pH-triggered size-switching layered delivery nanosystem is a safe and effective cancer treatment delivery platform that improves drug permeability and therapeutic efficacy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nano.2022.102592DOI Listing
July 2022

Correlation between serum immunoglobulin levels and retinal structure in patients with newly diagnosed Vogt‑Koyanagi‑Harada disease.

Mol Med Rep 2022 Sep 29;26(3). Epub 2022 Jul 29.

Department of Ophthalmology, Shanghai Xuhui Central Hospital, Shanghai 200031, P.R. China.

Immunoglobulins serve immunomodulatory roles in numerous autoimmune diseases. The aim of the present study was to investigate the correlations between serum Ig levels and retinal structural parameters in patients with newly diagnosed acute Vogt‑Koyanagi‑Harada (VKH) disease. A total of 138 participants were enrolled and the foveal thickness (FT), serous retinal detachment (SRD), sensory retinal thickness, central FT (CFT), cube volume (V) and cube average thickness (AT) were assessed by optical coherence tomography. The patients were divided, according to the extent of SRD, into a high‑detachment group (>500 µm) and a low‑detachment group (≤500 µm). Rate‑scattering turbidimetry was performed to measure the Ig levels. The high‑detachment group comprised 51 (36.96%) patients. The proportion of males was significantly greater in the high‑detachment group compared with the low‑detachment group (58.82 vs. 40.23%; P<0.05) and best‑corrected visual acuity was significantly worse in the high‑detachment group compared with the low‑detachment group (P<0.001). The IgE levels in the high‑detachment group were significantly greater compared with the low‑detachment group (P<0.05). FT, SRD, CFT, V and AT were significantly greater in the high‑detachment group compared with the low‑detachment group (P<0.001). The IgE levels were positively associated with SRD, CFT and AT (P<0.05). Multivariate binary logistic regression analysis demonstrated that male sex (B=2.447; P<0.05) and serum IgE levels (B=0.997, P<0.05) may be independent risk factors for severe SRD. The results of the present study demonstrated that males are more likely to develop severe SRD and that serum IgE levels were associated with the extent of detachment. These data suggested that IgE may be involved in the progression of VKH disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2022.12807DOI Listing
September 2022

Serum Serine and the Risk of All-Cause Mortality: A Nested Case-Control Study From the China Stroke Primary Prevention Trial (CSPPT).

Front Nutr 2022 12;9:946277. Epub 2022 Jul 12.

Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health, College of Food Sciences and Nutritional Engineering, China Agricultural University, Beijing, China.

Background: Serine plays a key role in numerous cellular processes, the levels and metabolism is therefore of critical importance. However, few data are available to illustrate the association of serine with long-term health effects, especially, the predictive value for long-term mortality.

Objective: This study was conducted to evaluate the relationship between serum serine levels and all-cause mortality in general hypertensive patients in a longitudinal cohort, and to examine the potential effect modifiers.

Methods: A nested case-control (NCC) study was conducted utilizing 20702 hypertensive participants from the China Stroke Primary Prevention Trial (CSPPT), a randomized, double-blind, actively controlled trial conducted from May 2008 to August 2013 in China. The current study included 291 cases of all-cause mortality and 291 controls matched on age (≤ 1 year), sex and treatment group. All-cause mortality was the main outcome in this analysis, which included death due to any reason.

Results: With the increase in serum serine levels, the risk of all-cause mortality first increased before flattening. After adjusting for related variables, the risk of mortality increased significantly with the increase of serum serine levels. Compared with group Q1, the mortality risk of group Q2, Q3 and Q4 were significantly increased [ORs, 95% CI: Q2: 2.32, (1.32-4.07); Q3: 2.59, (1.48-4.54); and Q4: 1.85, (1.07-3.22)]. In the exploratory analysis, we observed three effect modifiers, total homocysteine, 5-Methyltetrahydrofolate, and estimated glomerular filtration rate significantly modified the serum serine and all-cause mortality association.

Conclusion: Serum serine levels were significantly associated with an increased risk of all-cause mortality in hypertensive patients. Our results and findings, if confirmed further, suggest that serum serine should be considered as a marker for screening risk factors of mortality.

Clinical Trial Registration: [https://www.clinicaltrials.gov/ct2/show/study/NCT00794885.], identifier [CSPPT, NCT00794885].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fnut.2022.946277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315370PMC
July 2022

Lp2 improved LPS-induced liver injury through the TLR-4/MAPK/NFκB and Nrf2-HO-1/CYP2E1 pathways in mice.

Food Nutr Res 2022 5;66. Epub 2022 Jul 5.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China.

Background: Inflammatory liver diseases present a significant public health problem. Probiotics are a kind of living microorganisms, which can improve the balance of host intestinal flora, promote the proliferation of intestinal beneficial bacteria, inhibit the growth of harmful bacteria, improve immunity, reduce blood lipids and so on. Probiotics in fermented foods have attracted considerable attention lately as treatment options for liver injury.

Objective: The aim of this study was selected probiotic strain with well probiotic properties from naturally fermented foods and investigated the underlying mechanisms of screened probiotic strain on lipopolysaccharide (LPS)-induced liver injury, which provided the theoretical foundation for the development of probiotics functional food.

Design: The probiotic characteristics of Lp2 isolated from Chinese traditional fermented food were evaluated. Male KM mice were randomly assigned into three groups: normal chow (Control), LPS and LPS with Lp2. Lp2 were orally administered for 4 weeks before exposure to LPS. The liver injury of LPS-induced mice was observed through the evaluation of biochemical indexes, protein expression level and liver histopathology.

Results And Discussions: After treatment for 4 weeks, Lp2 administration significantly reduced the LPS-induced liver coefficient and the levels of serum or liver aspartate transaminase (AST), alanine aminotransferase (ALT), tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) and LPS, as well as decreasing the histological alterations and protein compared with the LPS group. Western-blotting results showed that Lp2 activated the signal pathway of TLR4/MAPK/NFκB/NRF2-HO-1/CYP2E1/Caspase-3 and regulated the expression of related proteins.

Conclusions: In summary, Lp2 suppressed the LPS-induced activation of inflammatory pathways, oxidative injury and apoptosis has the potential to be used to improve liver injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.29219/fnr.v66.5459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9287763PMC
July 2022

The Dynamic Characteristics of Myocardial Contractility and Extracellular Volume in Type 2 Diabetes Mellitus Mice Investigated by 7.0T Cardiac Magnetic Resonance.

J Clin Med 2022 Jul 22;11(15). Epub 2022 Jul 22.

Department of Radiology, Beijing Anzhen Hospital, Beijing Institute of Heart, Lung & Vascular Diseases, Capital Medical University, Beijing 100029, China.

Type 2 diabetes mellitus (T2DM) is associated with a high prevalence of diastolic dysfunction and congestive heart failure. A potential contributing factor is the accelerated accumulation of diffuse myocardial fibrosis and stiffness. Novel cardiac magnetic resonance (CMR) imaging techniques can identify both myocardial fibrosis and contractility quantitatively. This study aimed to investigate the dynamic characteristics of the myocardial strain and altered extracellular volume (ECV) fraction as determined by 7.0 T CMR in T2DM mice. C57Bl/6J mice were randomly divided into T2DM (fed a high-fat diet) and control (fed a normal diet) groups. They were scanned on 7.0 T MRI every 4 weeks until the end of week 24. The CMR protocol included multi-slice cine imaging to assess left ventricle strain and strain rate, and pre- and post-contrast T1 mapping images to quantify ECV. The ECV in the T2DM mice was significantly higher ( < 0.05) than that in the control group since week 12 with significantly impaired myocardial strain ( < 0.05). A significant linear correlation was established between myocardial strain and ECV ( < 0.001) and left ventricular-ejection fraction and ECV ( = 0.003). The results suggested that CMR feature tracking-derived myocardial strain analysis can assess functional abnormalities that may be associated with ECM alterations in diabetic cardiomyopathy, contributing to the study of diabetic therapy effects.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/jcm11154262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332454PMC
July 2022

A Multiscale Study of CFRP Based on Asymptotic Homogenization with Application to Mechanical Analysis of Composite Pressure Vessels.

Polymers (Basel) 2022 Jul 11;14(14). Epub 2022 Jul 11.

National Key Laboratory of Transit Physics, Nanjing University of Science and Technology, Nanjing 210094, China.

The application of composites is increasingly extensive due to their advanced properties while the analysis still remains complex on different scales. In this article, carbon fiber reinforced polymer (CFRP) is modeled via asymptotic homogenization employing a representative volume element (RVE) with periodic boundary conditions. A multiscale mechanical model of CFRP is established to bridge the microscopic model, mesoscopic model, and macroscopic model. According to asymptotic homogenization, the coefficients of the material constitutive equation are calculated with volume-averaged stress and strain. Using the homogenized materials properties of CFRP, the tensile experiments of composite layers with the layout of [(0∘/60∘/0∘/-60∘)4] are carried out to validate asymptotic homogenization method. The results indicated that the asymptotic homogenization approach can be used to calculate the homogenized elastic moduli and Poisson's ratio of the whole structure, where the numerical results are basically consistent with test data. The sequent homogenized CFRP laminate model is applied to the mechanical analysis of type III composite pressure vessels, whereby burst pressure is accurately predicted. This work might shed some light on multiscale analysis of composite pressure vessels.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym14142817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316031PMC
July 2022

Effect of the Class C Fly Ash on Low-Reactive Gold Mine Tailing Geopolymers.

Polymers (Basel) 2022 Jul 9;14(14). Epub 2022 Jul 9.

Department of Metallurgy Engineering, Universidad Nacional de San Agustín de Arequipa, Arequipa 04000, Peru.

Beneficiation of industrial wastes, such as mine tailings (MTs), through development of alternative eco-friendly geopolymer binders for construction composites offers a twofold environmental benefit, as it reduces the demand for cement and it increases the sustainability of industrial processes by creating a value-added product from an industrial byproduct. While MTs have the requisite composition for use as a geopolymer precursor, they are often low-reactive. This study explored the effect of Class C Fly Ash (FAc) on the geopolymerization of low-reactive gold MTs. A 10 M sodium hydroxide (NaOH) solution was used as the alkaline activator with four different concentrations of FAc (5, 10, 15 and 20 wt.%). The results indicated that the combination of FAc with the low-reactive gold MTs improved the physicochemical stability of the final geopolymerized samples, with a 95-120% increase in compressive strength, compared to the geopolymer samples of only low-reactive gold MTs. Although some of the strength improvement could be attributed to geopolymerization of the FAc itself, the presence of the FAc also improved the reactivity of the MTs, increasing the geopolymer production of the MTs. This study documents the positive effects of the FAc on gold MTs with low-calcium content and their conversion into sustainable inorganic composite geopolymers for the construction field.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym14142809DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317708PMC
July 2022

Vitamin K2 Improves Osteogenic Differentiation by Inhibiting STAT1 via the Bcl-6 and IL-6/JAK in C3H10 T1/2 Clone 8 Cells.

Nutrients 2022 Jul 18;14(14). Epub 2022 Jul 18.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Osteogenic activity of vitamin K (VK), a small molecular nutrient, has been suggested. However, the underlying mechanisms have not been fully elucidated. Therefore, this study aimed to explore the mechanisms by which VK promotes osteogenic differentiation. The effects of VK on osteogenic differentiation indicators were determined in C3H10 T1/2 clone 8 cells. The RNA-seq analysis was used to explore the hypothesis that VK promotes osteogenic differentiation. Small interfering RNA (siRNA) assay and plasmid transfection assay were used to determine the potential role of VK in the modulation of Bcl-6/STAT axis and IL-6/JAK/STAT signaling pathway. VK significantly increased alkaline phosphatase (ALP) activity, ALP, osteocalcin (OCN), and RUNX2 abundance, and RUNX2 protein expression. RNA-seq analysis showed that there were 314 differentially expressed genes (DEGs) upregulated and 1348 DEGs downregulated by VK. PPI analysis determined the top 10 hub genes upregulated or downregulated by VK. Overexpression of Bcl-6 increased osteogenic differentiation and decreased expression of STAT1. Administration with VK restored the inhibition by siBcl-6 in osteogenic differentiation. Knockdown of IL-6 decreased the mRNA levels of genes associated with the JAK/STAT signaling pathway, and increased markers of osteoblast differentiation. Furthermore, treatment with VK improved inhibition in osteogenic differentiation and decreased enhancement of JAK/STAT signaling pathway related genes by overexpression of IL-6. Our study suggests that VK could improve osteogenic differentiation via the Bcl-6/STAT axis and IL-6/JAK/STAT signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu14142934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316273PMC
July 2022

Characterization and Biomechanical Study of a Novel Magnesium Potassium Phosphate Cement.

Life (Basel) 2022 Jul 5;12(7). Epub 2022 Jul 5.

Senior Department of Orthopedics, The Fourth Medical Centre of Chinese PLA General Hospital, Beijing 100089, China.

Magnesium potassium phosphate cement (MKPC) has attracted considerable attention as a bone regeneration material. However, there are only a few reports on its biomechanical properties. To evaluate the biomechanical properties of MKPC, we compared the mechanical parameters of pedicle screws enhanced with either MKPC or polymethyl methacrylate (PMMA) bone cement. The results show that the maximum pull-out force of the pedicle screws was 417.86 ± 55.57 and 444.43 ± 19.89 N after MKPC cement setting for 30 min and 12 h, respectively, which was better than that of the PMMA cement. In fatigue tests, the maximum pull-out force of the MKPC cement group was 435.20 ± 7.96 N, whereas that of the PMMA cement in the control group was 346.80 ± 7.66 N. Furthermore, the structural characterization analysis of the MKPC cement revealed that its microstructure after solidification was an irregular tightly packed crystal, which improved the mechanical strength of the cement. The maximum exothermic temperature of the MKPC reaction was 45.55 ± 1.35 °C, the coagulation time was 7.89 ± 0.37 min, and the compressive strength was 48.29 ± 4.76 MPa, all of which meet the requirements of clinical application. In addition, the MKPC cement did not significantly inhibit cell proliferation or increase apoptosis, thus indicating good biocompatibility. In summary, MKPC exhibited good biomechanical properties, high initial strength, good biocompatibility, and low exothermic reaction temperature, demonstrating an excellent application potential in the field of orthopedics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/life12070997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320010PMC
July 2022

Reducing the Use of Disposable Plastics through Public Engagement Campaigns: An Experimental Study of the Effectiveness of Message Appeals, Modalities, and Sources.

Int J Environ Res Public Health 2022 Jul 6;19(14). Epub 2022 Jul 6.

Department of Economics, Hong Kong Baptist University, Hong Kong 999077, China.

This study examines how different ways of presenting information about the ecological threats stemming from the use of disposable plastics may affect people's willingness to reduce their use. To test our hypotheses, we used a 2 × 3 × 2 between-subjects experimental design, utilizing a sample of 1001 Hong Kong residents. The independent variables tested included: (a) message frame (gain vs. loss), (b) modality (text vs. image vs. infographic), and (c) information source (government vs. non-governmental organization). The findings demonstrate that the loss frame was more effective than the gain frame in persuading participants to reduce the use of disposable plastics. Furthermore, compared to image-based messages, text-based and infographic-based messages were more effective in promoting the reduction in the use of disposable plastics. For information sources, however, we found no main effect on behavioral intentions. However, this study still suggests an interaction effect of the loss frame and NGO source, as well as the interaction between text-based modality and government source, both leading to more positive outcomes. Furthermore, the study reveals that negative emotional responses mediate the effect of media frames on behavioral intentions. The findings offer useful insights for designing more effective communication campaigns aimed at curbing the use of disposable plastics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijerph19148273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319679PMC
July 2022

The Apelin-APJ axis alleviates LPS-induced pulmonary fibrosis and endothelial mesenchymal transformation in mice by promoting Angiotensin-Converting Enzyme 2.

Cell Signal 2022 Jul 23;98:110418. Epub 2022 Jul 23.

Institute of Hypoxia Medicine, School of Basic Medical Sciences,Wenzhou Medical University, Wenzhou, China. Electronic address:

Fibrotic alterations resulting from abnormal tissue repair after lung injury are responsible for the high mortality observed after acute respiratory distress syndrome. Therefore, the prevention and treatment of pulmonary fibrosis has been widely concerned. The Apelin-APJ axis plays an important role in the prevention and treatment of respiratory diseases and organ fibrosis. However, its underlying mechanism remains to be further studied. The aim of this study was to investigate whether the anti-pulmonary fibrosis effect of apelin-APJ axis is related to the activation of angiotensin-converting Enzyme 2 (ACE2). Here, we found that exogenous activation of the Apelin-APJ axis alleviates lipopolysaccharide (LPS)-induced pulmonary fibrosis in mice. In vitro studies revealed that Apelin-13 inhibited LPS-induced endothelial mesenchymal transition in lung microvascular endothelial cells, whereas [Ala13]-Apelin-13 (Apelin-APJ axis inhibitor) accelerated LPS-induced endothelial interstitial transformation in lung microvascular endothelial cells. Notably, angiotensin-converting enzyme 2 (ACE2) inhibitor blocks the beneficial effect of the Apelin-APJ axis activation on LPS-induced pulmonary fibrosis. This finding suggests that the Apelin-APJ axis inhibits pulmonary fibrosis by activating ACE2. Simultaneously, accumulating evidence suggests that ubiquitination may contribute to pulmonary fibrosis. Our study found that LPS increased the ubiquitination of ACE2 protein, whereas Apelin-13 inhibited it. In conclusion, exogenous activation of the Apelin-APJ axis improves LPS-induced pulmonary fibrosis in mice and may be a viable therapeutic target for pulmonary fibrosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cellsig.2022.110418DOI Listing
July 2022

Association of Health Insurance Literacy With Financial Hardship in Patients With Cancer.

JAMA Netw Open 2022 07 1;5(7):e2223141. Epub 2022 Jul 1.

Mayo Clinic, Rochester, Minnesota.

Importance: Patient-reported financial hardship is an increasing challenge in cancer care delivery. Health insurance literacy and its association with financial hardship in patients with cancer, especially after controlling for financial literacy, have not been well examined.

Objective: To examine the prevalence of and factors in the association between health insurance literacy and financial literacy as well as the overall and individual domains of financial hardship and their association with health insurance literacy, both independently and when adjusted for financial literacy, in patients with cancer.

Design, Setting, And Participants: This cross-sectional survey study recruited and enrolled patients from 2 separate ambulatory infusion centers at Mayo Clinic Arizona in Phoenix, Arizona, and the University of Mississippi Medical Center in Jackson, Mississippi. Adult patients aged 18 years or older were enrolled from December 2019 to February 2020 and from August to October 2020 at Mayo Clinic Arizona (n = 299) and from September 2020 through January 2021 at the University of Mississippi Medical Center (n = 105). Survey respondents received a $5 gift card.

Exposures: Surveys included questions about sociodemographic characteristics, health insurance literacy and financial literacy, financial knowledge, and financial hardship and its domains (material hardship, psychological hardship, and behavioral hardship).

Main Outcomes And Measures: Financial hardship was assessed using the COST-FACIT (Comprehensive Score for Financial Toxicity-Functional Assessment of Chronic Illness Therapy) measure and National Health Interview Survey questions to capture information about the domains of financial hardship. The Health Insurance Literacy Measure is a validated 21-item measure of a consumer's ability to select and use health insurance. Five questions from the National Financial Capability Study assessed financial literacy.

Results: A total of 404 participants were enrolled in the study. Median (IQR) age of the respondents was 63 (54-71) years, and 219 were women (54%), 307 were non-Hispanic White individuals (76%), 153 (38%) had private insurance, and 289 (72%) had solid tumors. Overall financial hardship (denoted by median COST-FACIT score <27 points) was reported by 49% (95% CI, 44%-53%) of the cohort. Prevalence of financial hardship was higher using the National Health Interview Survey questions, with 68% (95% CI, 63%-72%) of respondents reporting at least 1 hardship domain (n = 276). Sixty-six percent (95% CI, 60%-69%) of respondents (n = 265) had a high level of financial literacy. The mean (SD) Health Insurance Literacy Measure score was 64.9 (13.3) points. In multivariable analyses, each 10-point increase in the Health Insurance Literacy Measure score was associated with lower odds of financial hardship (odds ratio, 0.82; 95% CI, 0.68-0.99; P = .04). However, this association was no longer significant after adjusting for financial literacy.

Conclusions And Relevance: Results of this study showed that, despite a high level of health insurance literacy and financial literacy, the prevalence of financial hardship was high. Although there were lower odds of financial hardship with increased health insurance literacy, the association was no longer significant when financial literacy was added to the model, suggesting that a high level of financial literacy may help mitigate the adverse outcome of lower health insurance literacy levels in patients with cancer.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamanetworkopen.2022.23141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315419PMC
July 2022

Association of Visit-to-Visit Variability in Fasting Plasma Glucose with Digestive Cancer Risk.

Oxid Med Cell Longev 2022 13;2022:4530894. Epub 2022 Jul 13.

Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin 300211, China.

Background And Aims: The aim of this study is to investigate the association between visit-to-visit variability in fasting plasma glucose (FPG) and the risk of digestive cancers among individuals with and without diabetes.

Methods: Using data from Kailuan cohort, a prospective population-based study, individuals who had at least two measurements of FPG between 2006 and 2012 without prior cancer were included in this study. Four indexes of variability were used, including standard deviation (SD), coefficient of variation (CV), variability independent of the mean (VIM), and average successive variability (ARV). Cox regression was used to evaluate the relationship between the quartiles of FPG variability and digestive cancers.

Results: A total of 98,161 individuals were studied. Over a mean follow-up of 9.32 ± 0.81 years, 1103 individuals developed incident digestive cancer (1.21 per 1000 person-years). Compared to the individuals in the lowest quartile, those in the highest quartile of FPG variability by SD had 38.7% higher risk of developing overall digestive cancers after adjusting for the significant confounders (hazard ratio, 1.387; 95% confidence interval, 1.160-1.659; = 0.0003). Higher FPG variability was associated with significantly higher risks of colorectal cancer (fully adjusted HR 1.432, 95% CI [1.073-1.912], = 0.015) and pancreatic cancer (fully adjusted HR 2.105, 95% CI [1.024-4.329], = 0.043), but not liver cancer (fully adjusted HR 1.427, 95% CI [0.973-2.092], = 0.069) or esophageal and gastric cancer (fully adjusted HR 1.139, 95% CI [0.776-1.670], = 0.506). Subgroup analyses showed that individuals who were younger (<65 years), male, and those without diabetes experienced a predominantly higher risk of developing digestive cancers. Similar results were observed when using CV, VIM, and ARV.

Conclusions: FPG variability was significantly associated with increasing risk of digestive cancers, especially for pancreatic and colorectal cancer. Our study suggested a potential role of FPG variability in risk stratification of digestive cancers. Approaches that reduce FPG variability may lower the risks of incident digestive cancers among the general population. This trial is registered with ChiCTR-TNRC-11001489.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/4530894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301759PMC
July 2022

Performance of Machine Learning for Tissue Outcome Prediction in Acute Ischemic Stroke: A Systematic Review and Meta-Analysis.

Front Neurol 2022 8;13:910259. Epub 2022 Jul 8.

Department of Radiology, General Hospital of Northern Theater Command, Shenyang, China.

Machine learning (ML) has been proposed for lesion segmentation in acute ischemic stroke (AIS). This study aimed to provide a systematic review and meta-analysis of the overall performance of current ML algorithms for final infarct prediction from baseline imaging. We made a comprehensive literature search on eligible studies developing ML models for core infarcted tissue estimation on admission CT or MRI in AIS patients. Eleven studies meeting the inclusion criteria were included in the quantitative analysis. Study characteristics, model methodology, and predictive performance of the included studies were extracted. A meta-analysis was conducted on the dice similarity coefficient (DSC) score by using a random-effects model to assess the overall predictive performance. Study heterogeneity was assessed by Cochrane and Higgins tests. The pooled DSC score of the included ML models was 0.50 (95% CI 0.39-0.61), with high heterogeneity observed across studies ( 96.5%, < 0.001). Sensitivity analyses using the one-study removed method showed the adjusted overall DSC score ranged from 0.47 to 0.52. Subgroup analyses indicated that the DL-based models outperformed the conventional ML classifiers with the best performance observed in DL algorithms combined with CT data. Despite the presence of heterogeneity, current ML-based approaches for final infarct prediction showed moderate but promising performance. Before well integrated into clinical stroke workflow, future investigations are suggested to train ML models on large-scale, multi-vendor data, validate on external cohorts and adopt formalized reporting standards for improving model accuracy and robustness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2022.910259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305175PMC
July 2022

Heterogenous Subtypes of Late-Life Depression and Their Cognitive Patterns: A Latent Class Analysis.

Front Psychiatry 2022 6;13:917111. Epub 2022 Jul 6.

Clinical Research Division, Dementia Care and Research Center, Peking University Institute of Mental Health (Sixth Hospital), Beijing, China.

Background: Late-life depression (LLD), characterized by cognitive deficits, is considered heterogeneous across individuals. Previous studies have identified subtypes with diverse symptom profiles, but their cognitive patterns are unknown. This study aimed to investigate the subtypes of LLD and the cognitive profile of each group.

Methods: In total, 109 depressed older adults were enrolled. We performed latent class analysis using Geriatric Depression Scale items as indicators to generate latent classes. We compared the sociodemographic and clinical characteristics with cognitive functions between groups and conducted regression analysis to investigate the association between class membership and variables with significant differences.

Results: Two classes were identified: the "pessimistic" group was characterized by pessimistic thoughts and the "worried" group with a relatively high prevalence of worry symptoms. The two groups did not differ in sociodemographic characteristics. The "pessimistic" group showed a higher rate of past history of depression and lower age of onset. The "worried" group had more physical comorbidities and a higher rate of past history of anxiety. The "pessimistic" group was more impaired in general cognitive function, executive function, information processing speed, and attention. Lower general and executive functions were associated with the membership in the "pessimistic" group.

Conclusions: Subjects with pessimistic symptoms and subjects with a propensity to worry may form two distinct subtypes of late-life depression with different cognitive profiles. Further, the cognitive evaluation of subjects with pessimistic symptoms is of utmost importance.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpsyt.2022.917111DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298648PMC
July 2022

Retractorless Surgery for Petroclival Meningiomas via the Subtemporal Approach: A Try to Reduce Brain Retraction Injury.

Comput Math Methods Med 2022 14;2022:6436542. Epub 2022 Jul 14.

Department of Neurosurgery, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui 230036, China.

Purpose: To present our experience with retractorless surgery for resection of petroclival meningiomas (PCMs) via the subtemporal approach with routine operative instruments.

Methods: Clinical data of patients with PCMs who received surgical treatments via subtemporal approach were retrospectively analyzed. Patient demographics, duration of operation, extent of resection, postoperative brain injury rate, postoperative complication, and surgical outcome were reviewed.

Results: Twenty-nine consecutive patients with retractorless surgery via subtemporal approach performed between November 2018 and November 2021. The gross total resection rate was 82.8% ( = 24). The incidence of postoperative temporal lobe injury was 3.4% ( = 1). All the procedures were completed without fixed retraction or other specialized instruments.

Conclusions: Retractorless surgery via subtemporal approach is a reliable treatment option for PCMs, which can be completed with routine operative instruments.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/6436542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303107PMC
July 2022

The Chasm in Percutaneous Coronary Intervention and In-Hospital Mortality Rates Among Acute Myocardial Infarction Patients in Rural and Urban Hospitals in China: A Mediation Analysis.

Int J Public Health 2022 7;67:1604846. Epub 2022 Jul 7.

HEOA Group, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.

To determine to what extent the inequality in the ability to provide percutaneous coronary intervention (PCI) translates into outcomes for AMI patients in China. We identified 82,677 patients who had primary diagnoses of AMI and were hospitalized in Shanxi Province, China, between 2013 and 2017. We applied logistic regressions with inverse probability weighting based on propensity scores and mediation analyses to examine the association of hospital rurality with in-hospital mortality and the potential mediating effects of PCI. In multivariate models where PCI was not adjusted for, rural hospitals were associated with a significantly higher risk of in-hospital mortality (odds ratio [OR]: 1.19, 95% confidence interval [CI]: 1.03-1.37). However, this association was nullified (OR: 0.94, 95% CI: 0.81-1.08) when PCI was included as a covariate. Mediation analyses revealed that PCI significantly mediated 132.3% (95% CI: 104.1-256.6%) of the effect of hospital rurality on in-hospital mortality. The direct effect of hospital rurality on in-hospital mortality was insignificant. The results highlight the need to improve rural hospitals' infrastructure and address the inequalities of treatments and outcomes in rural and urban hospitals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/ijph.2022.1604846DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302370PMC
July 2022

Autophagy alleviates indium-induced programmed cell death in wheat roots.

J Hazard Mater 2022 Jul 14;439:129600. Epub 2022 Jul 14.

MOE Key Laboratory of Environment Remediation and Ecological Health, College of Natural Resource & Environmental Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:

Indium released in agroecosystems is becoming an emerging plant stressor, causing cellular damage and consequently crop yield losses. Previous studies have focused on indium-induced toxicity in plants, while plant adaptive responses to such emerging metal xenobiotics are poorly understood. Here, we explored the relationship of autophagy and programmed cell death (PCD) in wheat roots under indium stress. Indium treatment significantly decreased root activity and cell viability, and suppressed the length of root epidermal cells in the elongation zones. These symptoms may be associated with indium-induced PCD, as indium-stressed wheat roots displayed condensed and granular nuclei, increased number of TUNEL-positive nuclei, enhanced nuclear DNA fragmentation and caspase-3-like protease activity compared to untreated roots. Accordingly, indium enhanced the expression levels of TaMCA1 and TaMCA4, two major metacaspase genes mediated PCD in wheat plants. The enhanced expression of autophagy genes and formation of autophagosomes indicate that autophagy could regulate metabolic adaptation and repair stress-induced damage in wheat roots. Furthermore, reinforcing autophagy by activator rapamycin significantly decreased the number of TUNEL-positive nuclei and the activity of caspase-3-like protease, whereas inhibition of autophagy by 3-methyladenine aggravated diagnostic markers for PCD. These results together suggest that autophagy suppresses indium-induced PCD in wheat roots.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jhazmat.2022.129600DOI Listing
July 2022

Study on the Anti-demyelination Mechanism of Bu-Shen-Yi-Sui Capsule in the Central Nervous System Based on Network Pharmacology and Experimental Verification.

Mediators Inflamm 2022 12;2022:9241261. Epub 2022 Jul 12.

School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, China.

Methods: The potential active ingredients and corresponding potential targets of BSYS Capsule were obtained from the TCMSP, BATMAN-TCM, Swiss Target Prediction platform, and literature research. Disease targets of CNSD were explored through the GeneCards and the DisGeNET databases. The matching targets of BSYS in CNSD were identified from a Venn diagram. The protein-protein interaction (PPI) network was constructed using bioinformatics methods. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the mechanisms of BSYS. Furthermore, the neuroprotective effects of BSYS were evaluated using a cell model of hydrogen peroxide- (HO-) induced cell death in OLN-93 cells.

Results: A total of 59 potential bioactive components of BSYS Capsule and 227 intersection targets were obtained. Topological analysis showed that AKT had the highest connectivity degrees in the PPI network. Enrichment analysis revealed that the targets of BSYS in the treatment of CNSD were the PI3K-Akt and MAPK signaling pathway, among other pathways. GO analysis results showed that the targets were associated with various biological processes, including apoptosis, reactive oxygen species metabolic process, and response to oxidative stress, among others. The experimental results demonstrated that BSYS drug-containing serum alleviated the HO-induced increase in LDH, MDA, and ROS levels and reversed the decrease in SOD and mitochondrial membrane potential induced by HO. BSYS treatment also decreased the number of TUNEL (+) cells, downregulated Bcl-2 expression, and upregulated Bax and c-caspase-3 expression by promoting Akt phosphorylation.

Conclusion: BSYS Capsule alleviated HO-induced OLN-93 cell injury by increasing Akt phosphorylation to suppress oxidative stress and cell apoptosis. Therefore, BSYS can be potentially used for CNSD treatment. However, the results of this study are only derived from in vitro experiments, lacking the validation of in vivo animal models, which is a limitation of our study. We will further verify the underlying mechanisms of BSYS in animal experiments in the future.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2022/9241261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9296285PMC
July 2022

A sensitive UPLC-MS/MS method for simultaneous quantification of one-carbon metabolites & co-factors in human plasma.

J Pharm Biomed Anal 2022 Jul 16;219:114944. Epub 2022 Jul 16.

Jinan University College of Pharmacy, Guangzhou 510630, PR China. Electronic address:

One-carbon metabolism is an important metabolic pathway involved in many diseases, such as congenital malformations, tumours, cardiovascular diseases, anaemia, depression, cognitive diseases and liver disease. However, the current methods have specific defects in detecting and qualifying the related compounds of one-carbon metabolism. In this study, a validated method was established to simultaneously quantify 22 one-carbon metabolites & co-factors in human plasma and applied to the study of correlation between one-carbon metabolism and colorectal cancer in human plasma samples, which were from 44 healthy subjects and 55 colorectal cancer patients. The method used ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS), and the analytes included betaine, L-carnitine, L-cystathionine, L-cysteine, dimethylglycine, DL-homocysteic acid, homocysteine, methionine, pyridoxal hydrochloride, pyridoxamine dihydrochloride, pyridoxine dihydrochloride, S-(5'-Adenosyl)-L-homocysteine, serine, choline chloride, folic acid, glycine, pyridoxal phosphate monohydrate, riboflavin, taurine, 5-methyltetrahydrofolate, S-(5'-adenosyl)-L-methionine disulfate salt, trimethylamine oxide. The developed method was successfully applied to the quantification of 22 one-carbon metabolites & co-factors in human plasma from colorectal cancer patients and healthy individuals. The plasma concentrations of dimethylglycine was significantly decreased in the patients compared with the healthy individuals, while L-cystathionine was increased.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpba.2022.114944DOI Listing
July 2022
-->