Publications by authors named "Nan Tang"

104 Publications

Enhancing interactions between cells and hierarchical micro/nanostructured TiOfilms for efficient capture of circulating tumor cells.

Biomed Phys Eng Express 2021 Jul 26;7(5). Epub 2021 Jul 26.

School of Biomedical Engineering, Guangdong Medical University, Dongguan 523808, People's Republic of China.

Micro/nano hierarchical substrates with different micropillar spacings were designed and prepared for capture of tumor cells. The cell capture efficiency of hierarchical substrates with low-density micropillar arrays was similar to that of nanostructured substrate. Increasing the density of micopillars could significantly improve the capture efficiency. The maximum capture efficiency was achieved on the hierarchical substrate with micropillar spacings of 15m, but further reducing the micropillar spacings did not increase the cell capture efficiency. It was also found that hierarchical substrates with appropriate spacing of micropillars appeared more favorable for cell attachment and spreading, and thus enhancing the cell-material interaction. These results suggested that optimizing the micropillar arrays, such as the spacing between adjacent micropillars, could give full play to the synergistic effect of hierarchical hybrid micro/nanostructures in the interaction with cells. This study may provide promising guidance to design and optimize micro/nano hierarchical structures of biointerfaces for biomedical application.
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http://dx.doi.org/10.1088/2057-1976/ac14a3DOI Listing
July 2021

Tissue- and stage-specific landscape of the mouse translatome.

Nucleic Acids Res 2021 06;49(11):6165-6180

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.

The current understanding of how overall principles of translational control govern the embryo-to-adult transition in mammals is still far from comprehensive. Herein we profiled the translatomes and transcriptomes of six tissues from the mice at embryonic and adult stages and presented the first report of tissue- and stage-specific translational landscape in mice. We quantified the extent of gene expression divergence among different expression layers, tissues and stages, detected significant changes in gene composition and function underlying these divergences and revealed the changing architecture of translational regulation. We further showed that dynamic translational regulation can be largely achieved via modulation of translational efficiency. Translational efficiency could be altered by alternative splicing (AS), upstream and downstream open reading frames (uORFs and dORFs). We revealed AS-mediated translational repression that was exerted in an event type-dependent manner. uORFs and dORFs exhibited mutually exclusive usage and the opposing effects of translational regulation. Furthermore, we discovered many novel microproteins encoded by long noncoding RNAs and demonstrated their regulatory potential and functional relevance. Our data and analyses will facilitate a better understanding of the complexity of translation and translational regulation across tissue and stage spectra and provide an important resource to the translatome research community.
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http://dx.doi.org/10.1093/nar/gkab482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216458PMC
June 2021

Associations between risk factors for cardiovascular diseases and frailty among community-dwelling older adults in Lanzhou, China.

Int J Nurs Sci 2021 Apr 25;8(2):168-174. Epub 2021 Mar 25.

School of Nursing, Lanzhou University, Lanzhou, China.

Objectives: To examine the relationship between cardiovascular disease risk factors and frailty in a sample of older Chinese adults.

Methods: A total of 458 community-dwelling older adults (≥65 years) in Lanzhou, Gansu Province of China participated in a cross-sectional survey. Their status was evaluated in terms of frailty phenotype (unintentional weight loss, exhaustion, low activity levels, slowness and weakness). Participants were categorized as not frail, prefrail or frail. Cardiovascular disease risk factors that were assessed included: blood pressure, body mass index, waist circumference, blood glucose, total cholesterol, triglycerides, low-density lipoproteins and high-density lipoproteins.

Results: Individuals with obesity had an increased risk of prefrailty (: 2.26; 95% CI: 1.05, 4.84). Hypertension was inversely associated with frailty among the participants (: 0.31; 95% CI: 0.11, 0.87) after adjusting for covariates.

Conclusions: The findings suggest that much more attention should be paid to weight control of the elderly in the community for preventing them from transition to prefrailty or frailty. Active prevention and control of cardiovascular diseases among the community-dwelling elder are still of great importance.
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http://dx.doi.org/10.1016/j.ijnss.2021.03.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105554PMC
April 2021

The diversity of adult lung epithelial stem cells and their niche in homeostasis and regeneration.

Sci China Life Sci 2021 Apr 30. Epub 2021 Apr 30.

National Institute of Biological Sciences, Beijing, 102206, China.

The adult lung, a workhorse for gas exchange, is continually subjected to a barrage of assaults from the inhaled particles and pathogens. Hence, homeostatic maintenance is of paramount importance. Epithelial stem cells interact with their particular niche in the adult lung to orchestrate both natural tissue rejuvenation and robust post-injury regeneration. Advances in single-cell sequencing, lineage tracing, and living tissue imaging have deepened our understanding about stem cell heterogeneities, transition states, and specific cell lineage markers. In this review, we provided an overview of the known stem/progenitor cells and their subpopulations in different regions of the adult lung, and explored the regulatory networks in stem cells and their respective niche which collectively coordinated stem cell quiescence and regeneration states. We finally discussed relationships between dysregulated stem cell function and lung disease.
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http://dx.doi.org/10.1007/s11427-020-1902-3DOI Listing
April 2021

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Front Pharmacol 2020 18;11:563433. Epub 2021 Jan 18.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Psoriasis is a chronic inflammatory skin disease that is associated with multiple coexisting conditions. Extensive literature suggests that psoriasis is a T-cell-mediated condition, and its pathogenesis is related to dysfunction of the immune system. Myeloid-derived suppressor cells (MDSCs) are a group of heterogeneous myeloid cells that have suppressive effects on T cells. MDSCs are present at very low levels in healthy individuals but can substantially expand in tumours or inflammatory conditions. PSORI-CM02, a Chinese medical formula designed based on the Chinese medicine theory (), has been prescribed extensively for psoriasis therapy and shows a stable clinical effect and safety. This study discusses the mechanisms of MDSCs involved in disease development and therapeutic progress. Our data provides evidence that monocytic myeloid-derived suppressor cells (M-MDSCs) play a role in IMQ-induced psoriatic dermatitis. Functional characterization and correlation analysis indicated that MDSCs are positively correlated with Th17 cells. PSORI-CM02 alleviated IMQ-induced psoriatic dermatitis and suppressed the proliferation of Th17 cells via M-MDSC-induced Arg1 upregulation, suggesting M-MDSCs could be a novel therapeutic target for psoriasis, and PSORI-CM02 exerted its effects via the perturbation of M-MDSCs and Th17 cell crosstalk.
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http://dx.doi.org/10.3389/fphar.2020.563433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7847847PMC
January 2021

IMPERFECTIVE EXINE FORMATION (IEF) is required for exine formation and male fertility in Arabidopsis.

Plant Mol Biol 2021 Apr 22;105(6):625-635. Epub 2021 Jan 22.

College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.

Key Message: IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well studied, the transportation of it remains elusive. To understand it, we analyzed the gene expression pattern in tapetal microdissection data, and investigated the potential transporter genes that are putatively regulated by ABORTED MICROSPORES (AMS). Among these genes, we identified IMPERFECTIVE EXINE FORMATION (IEF) that is important for exine formation. Compared to the wild type, ief mutants exhibit severe male sterility and pollen abortion, suggesting IEF is crucial for pollen development and male fertility. Using both scanning and transmission electron microscopes, we showed that exine structure was not well defined in ief mutant. The transient expression of IEF-GFP driven by the 35S promoter indicated that IEF-GFP was localized in plasma membrane. Furthermore, AMS can specifically activate the expression of promoterIEF:LUC in vitro, which suggesting AMS regulates IEF for exine formation. The expression of ATP-BINDING CASSETTE TRANSPORTER G26 (AGCB26) was not affected in ief mutants. In addition, SEM and TEM data showed that the sporopollenin deposition is more defective in abcg26/ief-2 than that of in abcg26, which suggesting that IEF is involved in an independent sporopollenin transportation pathway. This work reveal a novel gene, IEF regulated by AMS that is essential for exine formation.
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http://dx.doi.org/10.1007/s11103-020-01114-8DOI Listing
April 2021

6-Gingerol protects against cardiac remodeling by inhibiting the p38 mitogen-activated protein kinase pathway.

Acta Pharmacol Sin 2021 Jan 18. Epub 2021 Jan 18.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

6-Gingerol, a pungent ingredient of ginger, has been reported to possess anti-inflammatory and antioxidant activities, but the effect of 6-gingerol on pressure overload-induced cardiac remodeling remains inconclusive. In this study, we investigated the effect of 6-gingerol on cardiac remodeling in in vivo and in vitro models, and to clarify the underlying mechanisms. C57BL/6 mice were subjected to transverse aortic constriction (TAC), and treated with 6-gingerol (20 mg/kg, ig) three times a week (1 week in advance and continued until the end of the experiment). Four weeks after TAC surgery, the mice were subjected to echocardiography, and then sacrificed to harvest the hearts for analysis. For in vitro study, neonatal rat cardiomyocytes and cardiac fibroblasts were used to validate the protective effects of 6-gingerol in response to phenylephrine (PE) and transforming growth factor-β (TGF-β) challenge. We showed that 6-gingerol administration protected against pressure overload-induced cardiac hypertrophy, fibrosis, inflammation, and dysfunction in TAC mice. In the in vitro study, we showed that treatment with 6-gingerol (20 μM) blocked PE-induced-cardiomyocyte hypertrophy and TGF-β-induced cardiac fibroblast activation. Furthermore, 6-gingerol treatment significantly decreased mitogen-activated protein kinase p38 (p38) phosphorylation in response to pressure overload in vivo and extracellular stimuli in vitro, which was upregulated in the absence of 6-gingerol treatment. Moreover, transfection with mitogen-activated protein kinase kinase 6 expressing adenoviruses (Ad-MKK6), which specifically activated p38, abolished the protective effects of 6-gingerol in both in vitro and in vivo models. In conclusion, 6-gingerol improves cardiac function and alleviates cardiac remodeling induced by pressure overload in a p38-dependent manner. The present study demonstrates that 6-gingerol is a promising agent for the intervention of pathological cardiac remodeling.
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http://dx.doi.org/10.1038/s41401-020-00587-zDOI Listing
January 2021

Stem cells in pulmonary alveolar regeneration.

Authors:
Huijuan Wu Nan Tang

Development 2021 01 18;148(2). Epub 2021 Jan 18.

National Institute of Biological Sciences, Beijing 102206, China

The lungs are constantly exposed to the external environment and are therefore vulnerable to insults that can cause infection and injury. Maintaining the integrity and barrier function of the lung epithelium requires complex interactions of multiple cell lineages. Elucidating the cellular players and their regulation mechanisms provides fundamental information to deepen understanding about the responses and contributions of lung stem cells. This Review focuses on advances in our understanding of mammalian alveolar epithelial stem cell subpopulations and discusses insights about the regeneration-specific cell status of alveolar epithelial stem cells. We also consider how these advances can inform our understanding of post-injury lung repair processes and lung diseases.
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http://dx.doi.org/10.1242/dev.193458DOI Listing
January 2021

Characterization of the complete chloroplast genome of (Liliaceae).

Mitochondrial DNA B Resour 2020 Jun 8;5(3):2362-2363. Epub 2020 Jun 8.

College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.

The chloroplast genome and evolutionary relationship analysis of L. could provide fundamental genetic reference for its molecular breeding and biological research. The complete chloroplast genome of was sequenced and reported here. Its chloroplast genome was 151,744 bp in length, containing a pair of inverted repeated regions (26,354 bp) which were separated by a large single copy region of 81,794 bp, and a small single copy region of 17,242 bp. Moreover, a total of 133 functional genes were annotated, including 87 mRNA, 38 tRNA genes, and 8 rRNA genes.The phylogenetic relationships of 16 species indicated that was closely related to .
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http://dx.doi.org/10.1080/23802359.2020.1773333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7783151PMC
June 2020

Complete chloroplast genome of (Liliaceae).

Mitochondrial DNA B Resour 2020 Jun 8;5(3):2360-2361. Epub 2020 Jun 8.

State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.

The complete chloroplast genome of was sequenced and reported here. The circular genome of is 152,062 bp in length and contains 133 functional genes consisting of 87 coding sequences, 38 tRNA genes, and 8 rRNA genes. With 1 species from Smilacaceae and 1 species from Alstroemeriaceae as outgroup, phylogenetic relationships of 8 Liliaceae species based on their chloroplast genomes indicated that is closest to .
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http://dx.doi.org/10.1080/23802359.2020.1773331DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782963PMC
June 2020

Down-regulated expression of transient receptor potential ankyrin 1 in lichen simplex chronicus.

Ann Palliat Med 2020 Nov 10;9(6):3757-3765. Epub 2020 Nov 10.

Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: Lichen simplex chronicus (LSC) is an acquired chronic dermatosis that is often accompanied by severe paroxysmal pruritus, and its pathogenesis to date is still unclear. Transient receptor potential channel A1 (TRPA1) is a non-selective cation channel. TRPA1 is widely expressed in skin, sensory neurons, and various other tissues, along with various biological functions and activation mechanisms. This study aimed to explore the changes in TRPA1 expression in human LSC and provide evidence for further research on the role of TRPA1 in chronic pruritus.

Methods: A total of 21 skin lesion specimens from LSC patients with skin pruritus lasting more than 6 weeks, and 28 normal skin tissue specimens through the skin biopsy from June 2018 to February 2019 were collected. The expression of TRPA1 in these skin tissues was evaluated through western blotting, quantitative reverse transcription PCR (qRT-PCR), and immunohistochemical analysis. The changes of inflammatory mediators, including interleukin (IL)-6, IL-13, endothelin-1 (ET-1), and thymic stromal lymphopoietin (TSLP), as well as substance P, are also analyzed by qRT-PCR. TRPA1 was detected using immunohistochemistry for all skin layers, the basal layer, and around the blood vessels of the dermis.

Results: The expression of TRPA1 in LSC specimens was significantly decreased as compared with that in the normal specimens (P<0.05). Also, TRPA1 protein levels were consistently decreased in LSC specimens (P<0.05). Simultaneously, the mRNA expressions of TRPA1, IL-6, IL-13, ET-1, TSLP, and substance P presented with no significant differences between LSC and normal specimens.

Conclusions: TRPA1 expression is proved downregulated in skin lesions of LSC patients with skin pruritus, indicating that TRPA1 serves as a crucial role in the pathogenesis of human LSC.
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http://dx.doi.org/10.21037/apm-20-1712DOI Listing
November 2020

TLR9 deficiency alleviates doxorubicin-induced cardiotoxicity via the regulation of autophagy.

J Cell Mol Med 2020 09 9;24(18):10913-10923. Epub 2020 Aug 9.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Doxorubicin is a commonly used anthracycline chemotherapeutic drug. Its application for treatment has been impeded by its cardiotoxicity as it is detrimental and fatal. DNA damage, cardiac inflammation, oxidative stress and cell death are the critical links in DOX-induced myocardial injury. Previous studies found that TLR9-related signalling pathways are associated with the inflammatory response of cardiac myocytes, mitochondrial dysfunction and cardiomyocyte death, but it remains unclear whether TLR9 could influence DOX-induced heart injury. Our current data imply that DOX-induced cardiotoxicity is ameliorated by TLR9 deficiency both in vivo and in vitro, manifested as improved cardiac function and reduced cardiomyocyte apoptosis and oxidative stress. Furthermore, the deletion of TLR9 rescued DOX-induced abnormal autophagy flux in vivo and in vitro. However, the inhibition of autophagy by 3-MA abolished the protective effects of TLR9 deletion on DOX-induced cardiotoxicity. Moreover, TLR9 ablation suppressed the activation of p38 MAPK during DOX administration and may promote autophagy via the TLR9-p38 MAPK signalling pathway. Our study suggests that the deletion of TLR9 exhibits a protective effect on doxorubicin-induced cardiotoxicity by enhancing p38-dependent autophagy. This finding could be used as a basis for the development of a prospective therapy against DOX-induced cardiotoxicity.
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http://dx.doi.org/10.1111/jcmm.15719DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7521247PMC
September 2020

Critical roles of macrophages in pressure overload-induced cardiac remodeling.

J Mol Med (Berl) 2021 01 31;99(1):33-46. Epub 2020 Oct 31.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China.

Macrophages are integral components of the mammalian heart that show extensive expansion in response to various internal or external stimuli. After the onset of sustained pressure overload (PO), the accumulation of cardiac macrophages through local macrophage proliferation and monocyte migration has profound effects on the transition to cardiac hypertrophy and remodeling. In this review, we describe the heterogeneity and diversity of cardiac macrophages and summarize the current understanding of the important roles of macrophages in PO-induced cardiac remodeling. In addition, the possible mechanisms involved in macrophage modulation are also described. Finally, considering the significant effects of cardiac macrophages, we highlight their emerging role as therapeutic targets for alleviating pathological cardiac remodeling after PO.
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http://dx.doi.org/10.1007/s00109-020-02002-wDOI Listing
January 2021

Integrative analysis of transcriptomic and proteomic changes related to male sterility in .

Physiol Mol Biol Plants 2020 Oct 25;26(10):2061-2074. Epub 2020 Sep 25.

Plateau Flower Research Centre, Department of Agriculture and Husbandry, Qinghai University, Xining, 810016 People's Republic of China.

Male sterile and male fertile two-type lines are important in heterosis utilization and breeding in , but the genes and pathways involved in male sterility are poorly understood. To explore these topics, transcriptome data (by RNA-seq) and proteome data (by iTRAQ) were gathered from flower buds of the male sterile line 'MS2-2' and male fertile line 'MF2-2' and integrated for a better understanding of the underlying molecular mechanisms of male sterility in . The RNA-seq procedure generated 285,139,740 clean reads and 63359 unigenes and 6640 differentially expressed genes (DEGs) were identified, of which 4136 were downregulated and 2504 were upregulated in 'MS2-2'. DEGs related to flower development, pollen development, pollen wall assembly, endogenous hormones and transcription factors were identified. The iTRAQ analysis identified 3950 proteins in total; 789 were differentially expressed proteins (381 upregulated, 408 downregulated), which were mainly annotated to the Ribosome, Carbon metabolism and Biosynthesis of amino acids pathways. An association analysis revealed strong correlation (r Pearson = 0.6019) between the transcriptomic and proteomic data, and 256 and 34 proteins showed the same and opposite expression patterns with regard to their transcripts, respectively. Pathways such as photosynthesis, fatty acid biosynthesis and phenylpropanoid biosynthesis which influence tapetum and pollen development in male sterile plants, were significantly enriched at the transcript and protein levels. Most genes involved in these pathways were downregulated in 'MS2-2'. The low expression of these genes or functional loss of proteins could be associated with flower development, pollen development and related to changes in fertility in . This study provided transcriptomic and proteomic information for that could illuminate the mechanism of male sterility.
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http://dx.doi.org/10.1007/s12298-020-00886-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7548268PMC
October 2020

The Chemosensitizing Role of Metformin in Anti-Cancer Therapy.

Anticancer Agents Med Chem 2021 ;21(8):949-962

Jiangxi Province Key Laboratory of Tumor Pathogens and Molecular Pathology and Department of Pathophysiology, Schools of Basic Medical Sciences, Nanchang University, Nanchang 330006, China.

Chemoresistance, which leads to the failure of chemotherapy and further tumor recurrence, presents the largest hurdle for the success of anti-cancer therapy. In recent years, metformin, a widely used first-line antidiabetic drug, has attracted increasing attention for its anti-cancer effects. A growing body of evidence indicates that metformin can sensitize tumor responses to different chemotherapeutic drugs, such as hormone modulating drugs, anti-metabolite drugs, antibiotics, and DNA-damaging drugs via selective targeting of Cancer Stem Cells (CSCs), improving the hypoxic microenvironment, and by suppressing tumor metastasis and inflammation. In addition, metformin may regulate metabolic programming, induce apoptosis, reverse Epithelial to Mesenchymal Transition (EMT), and Multidrug Resistance (MDR). In this review, we summarize the chemosensitization effects of metformin and focus primarily on its molecular mechanisms in enhancing the sensitivity of multiple chemotherapeutic drugs, through targeting of mTOR, ERK/P70S6K, NF-κB/HIF-1 α, and Mitogen- Activated Protein Kinase (MAPK) signaling pathways, as well as by down-regulating the expression of CSC genes and Pyruvate Kinase isoenzyme M2 (PKM2). Through a comprehensive understanding of the molecular mechanisms of chemosensitization provided in this review, the rationale for the use of metformin in clinical combination medications can be more systematically and thoroughly explored for wider adoption against numerous cancer types.>.
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http://dx.doi.org/10.2174/1871520620666200918102642DOI Listing
January 2021

Optical and Magnetic Properties of Ag-Ni Bimetallic Nanoparticles Assembled via Pulsed Laser-Induced Dewetting.

ACS Omega 2020 Aug 21;5(30):19285-19292. Epub 2020 Jul 21.

Department of Materials Science and Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.

Pulsed laser-induced dewetting (PLiD) of AgNi thin films results in phase-separated bimetallic nanoparticles with size distributions that depend on the initial thin film thickness. Co-sputtering of Ag and Ni is used to generate the as-deposited (AD) nanogranular supersaturated thin films. The magnetic and optical properties of the AD thin films and PLiD nanoparticles are characterized using a vibrating sample magnetometer, optical absorption spectroscopy, and electron energy loss spectroscopy (EELS). Magnetic measurements demonstrate that AgNi nanoparticles are ferromagnetic at room temperature when the nanoparticle diameters are >20 nm and superparamagnetic <20 nm. Optical measurements show that all nanoparticle size distributions possess a local surface plasmon resonance (LSPR) peak that red-shifts with increasing diameter. Following PLiD, a Janus nanoparticle morphology is observed in scanning transmission electron microscopy, and low-loss EELS reveals size-dependent Ag and Ni LSPR dipole modes, while higher order modes appear only in the Ag hemisphere. PLiD of Ag-Ni thin films is shown to be a viable technique to generate bimetallic nanoparticles with both magnetic and plasmonic functionality.
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http://dx.doi.org/10.1021/acsomega.0c02894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7409265PMC
August 2020

The Roles of Noncardiomyocytes in Cardiac Remodeling.

Int J Biol Sci 2020 2;16(13):2414-2429. Epub 2020 Jul 2.

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, RP China.

Cardiac remodeling is a common characteristic of almost all forms of heart disease, including cardiac infarction, valvular diseases, hypertension, arrhythmia, dilated cardiomyopathy and other conditions. It is not merely a simple outcome induced by an increase in the workload of cardiomyocytes (CMs). The remodeling process is accompanied by abnormalities of cardiac structure as well as disturbance of cardiac function, and emerging evidence suggests that a wide range of cells in the heart participate in the initiation and development of cardiac remodeling. Other than CMs, there are numerous noncardiomyocytes (non-CMs) that regulate the process of cardiac remodeling, such as cardiac fibroblasts and immune cells (including macrophages, lymphocytes, neutrophils, and mast cells). In this review, we summarize recent knowledge regarding the definition and significant effects of various non-CMs in the pathogenesis of cardiac remodeling, with a particular emphasis on the involved signaling mechanisms. In addition, we discuss the properties of non-CMs, which serve as targets of many cardiovascular drugs that reduce adverse cardiac remodeling.
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http://dx.doi.org/10.7150/ijbs.47180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7378633PMC
July 2020

Corrigendum: Microbial Community Analysis of Saliva and Biopsies in Patients With Oral Lichen Planus.

Front Microbiol 2020 26;11:1282. Epub 2020 Jun 26.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.

[This corrects the article on p. 629 in vol. 11, PMID: 32435231.].
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http://dx.doi.org/10.3389/fmicb.2020.01282DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7332857PMC
June 2020

Pulmonary alveolar regeneration in adult COVID-19 patients.

Cell Res 2020 08 6;30(8):708-710. Epub 2020 Jul 6.

National Institute of Biological Sciences, Beijing, 102206, China.

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http://dx.doi.org/10.1038/s41422-020-0369-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338112PMC
August 2020

New insights into BMP9 signaling in organ fibrosis.

Eur J Pharmacol 2020 Sep 20;882:173291. Epub 2020 Jun 20.

Department of Pathophysiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China. Electronic address:

Fibrosis is a common endpoint for chronic organic diseases. Many signaling pathways and cytokines are involved in the development of fibrosis. Recently, bone morphogenetic protein 9 (BMP9), a member of transforming growth factor-beta (TGF-β) superfamily, has been considered as a pivotal regulator in tissue fibrosis, and exerts its diverse effects on the activation of fibroblasts and the development of fibrotic diseases. BMP9 exhibits its functional roles largely through binding to type I BMP receptor activin receptor-like kinase 1 (ALK1), and then directly activates small mother against decapentaplegic (Smad) signaling which promotes or limits the expressions of pro-fibrotic genes. Accumulating studies have demonstrated that the aberrant BMP9/ALK1/Smad signaling pathway affects the fibrogenesis of various organs, but the exact role of BMP9 in fibrosis remains elusive and debatable. In the present review, reports from in vitro, in vivo and clinical studies regarding the functions and underlying mechanisms of the BMP9 signaling in tissue fibrosis, are widely summarized and discussed. In addition, the major components of the BMP9 signaling pathway and the potential interventions targeting this pathway are also described. This review will enrich our understanding of the BMP9 signaling pathway in organ fibrosis, and provide a novel clue for the potential interventions of organ fibrosis.
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http://dx.doi.org/10.1016/j.ejphar.2020.173291DOI Listing
September 2020

Review of Research on the Role of Irisin in Tumors.

Onco Targets Ther 2020 19;13:4423-4430. Epub 2020 May 19.

Department of Hepatobiliary Surgery, The Affiliated Qingdao Municipal Hospital of Qingdao University, Qingdao 266000, Shandong Province, People's Republic of China.

Irisin is a newly discovered exercise-induced cytokine, produced by the proteolytic hydrolysis of fibronectin type III domain-containing protein 5 (FNDC5). Irisin is widely distributed in the human body and is involved in the browning of white adipose tissue, improving insulin resistance, improving cognitive function, and regulating bone metabolism. Recent studies have shown that irisin concentration is elevated in a variety of tumor tissues as compared with that in normal tissues. However, irisin has different effects on the proliferation and apoptosis of tumor cells in breast cancer, lung cancer, and liver cancer through various mechanisms. Irisin plays an important role in the occurrence, development, and metastasis of different tumors, suggesting that irisin can be used as a potential target for tumor diagnosis and treatment. Therefore, studying the expression and function of irisin in tumors may be of great significance for the prevention and treatment of tumors. This article reviews the research progress on the role of irisin in tumors.
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http://dx.doi.org/10.2147/OTT.S245178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245464PMC
May 2020

Microbial Community Analysis of Saliva and Biopsies in Patients With Oral Lichen Planus.

Front Microbiol 2020 5;11:629. Epub 2020 May 5.

Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.

The specific etiology and pathogenesis of oral lichen planus (OLP) remain elusive, and microbial dysbiosis may play an important role in OLP. We evaluated the saliva and tissue bacterial community of patients with OLP and identified the colonization of bacteria in OLP tissues. The saliva ( = 60) and tissue ( = 24) samples from OLP patients and the healthy controls were characterized by 16S rDNA gene sequencing and the bacterial signals in OLP tissues were detected by fluorescence hybridization (FISH) targeting the bacterial 16S rDNA gene. Results indicate that the OLP tissue microbiome was different from the microbiota of OLP saliva. Compared with the healthy controls, and were higher in OLP saliva, while - and were higher in OLP tissues, whereas seven taxa, including Carnobacteriaceae, Flavobacteriaceae, and , were enriched in both saliva and tissues of OLP patients. Furthermore, FISH found that the average optical density (AOD) of bacteria in the lamina propria of OLP tissues was higher than that of the healthy controls, and the AOD of bacteria in OLP epithelium and lamina propria was positively correlated. These data provide a different perspective for future investigation on the OLP microbiome.
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http://dx.doi.org/10.3389/fmicb.2020.00629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219021PMC
May 2020

SARS-CoV-2 and viral sepsis: observations and hypotheses.

Lancet 2020 05 17;395(10235):1517-1520. Epub 2020 Apr 17.

Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Center of Respiratory Medicine, National Clinical Research Center for Respiratory Diseases, Beijing, China; Institute of Respiratory Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. Electronic address:

Since the outbreak of coronavirus disease 2019 (COVID-19), clinicians have tried every effort to understand the disease, and a brief portrait of its clinical features have been identified. In clinical practice, we noticed that many severe or critically ill COVID-19 patients developed typical clinical manifestations of shock, including cold extremities and weak peripheral pulses, even in the absence of overt hypotension. Understanding the mechanism of viral sepsis in COVID-19 is warranted for exploring better clinical care for these patients. With evidence collected from autopsy studies on COVID-19 and basic science research on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and SARS-CoV, we have put forward several hypotheses about SARS-CoV-2 pathogenesis after multiple rounds of discussion among basic science researchers, pathologists, and clinicians working on COVID-19. We hypothesise that a process called viral sepsis is crucial to the disease mechanism of COVID-19. Although these ideas might be proven imperfect or even wrong later, we believe they can provide inputs and guide directions for basic research at this moment.
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http://dx.doi.org/10.1016/S0140-6736(20)30920-XDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7164875PMC
May 2020

Efficacy and safety of intensity-modulated radiotherapy alone versus intensity-modulated radiotherapy plus chemotherapy for treatment of intermediate-risk nasopharyngeal carcinoma.

Radiat Oncol 2020 Mar 16;15(1):66. Epub 2020 Mar 16.

Department of Radiation Oncology, Affiliated Hospital of Guilin Medical University, 15 Lequn Road, Guilin, 541001, People's Republic of China.

Background: This study directs to evaluate the efficacy and safety of intensity-modulated radiotherapy (IMRT) alone versus IMRT plus chemotherapy in intermediate-risk NPC (stage II and TNM).

Methods: A total of 124 patients with stage II and TNM NPC were pair-matched (1:1 ratio) to form two groups: an IMRT-alone group and an IMRT/chemotherapy group. Survival outcomes (overall survival [OS], disease-free survival [DFS], locoregional relapse-free survival [LRRFS], distant metastasis-free survival [DMFS]) and treatment-related grade 3-4 acute toxicity events were compared between the groups.

Results: Survival outcomes for patients with stage II and TNM NPC were quiet comparable between patients treated with IMRT alone versus patients treated with IMRT/chemotherapy: 5-year OS was 91.9% vs. 90.3%, respectively (P = 0.727); DFS was 87.1% vs. 88.7%, respectively (P = 0.821); LRFFS was 96.8% vs. 95.2%, respectively (P = 0.646), and DMFS was 91.9% vs. 91.5%, respectively (P = 0.955). Grade 3 acute toxicities were significantly higher with IMRT/chemotherapy than with IMRT alone: mucositis, 15% vs. 5% (P = 0.004); leukopenia/neutropenia, 8% vs. 1% (P <  0.015); and nausea/vomiting, 22% vs. 3% (P <  0.001).

Conclusion: For intermediate-risk (stage II and TNM) NPC patients, the addition of chemotherapy to IMRT does not appear to provide any survival benefit. Moreover, grade 3 acute toxicities are also more common in patients receiving IMRT plus chemotherapy.
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http://dx.doi.org/10.1186/s13014-020-01508-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074987PMC
March 2020

Progressive Pulmonary Fibrosis Is Caused by Elevated Mechanical Tension on Alveolar Stem Cells.

Cell 2020 01 19;180(1):107-121.e17. Epub 2019 Dec 19.

National Institute of Biological Sciences, Beijing 102206, China; Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 100084, China. Electronic address:

Fibrosis can develop in most organs and causes organ failure. The most common type of lung fibrosis is known as idiopathic pulmonary fibrosis, in which fibrosis starts at the lung periphery and then progresses toward the lung center, eventually causing respiratory failure. Little is known about the mechanisms underlying the pathogenesis and periphery-to-center progression of the disease. Here we discovered that loss of Cdc42 function in alveolar stem cells (AT2 cells) causes periphery-to-center progressive lung fibrosis. We further show that Cdc42-null AT2 cells in both post-pneumonectomy and untreated aged mice cannot regenerate new alveoli, resulting in sustained exposure of AT2 cells to elevated mechanical tension. We demonstrate that elevated mechanical tension activates a TGF-β signaling loop in AT2 cells, which drives the periphery-to-center progression of lung fibrosis. Our study establishes a direct mechanistic link between impaired alveolar regeneration, mechanical tension, and progressive lung fibrosis.
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http://dx.doi.org/10.1016/j.cell.2019.11.027DOI Listing
January 2020

Efficacy and safety of bevacizumab-based maintenance therapy in metastatic colorectal cancer: A meta-analysis.

Medicine (Baltimore) 2019 Dec;98(50):e18227

The Fuling Center Hospital of Chongqing City.

Objective: To identify the optimal treatment strategy after first-line induction chemotherapy for metastatic colorectal cancer (mCRC).

Methods: We conducted a meta-analysis of randomized controlled trials comparing bevacizumab-based maintenance therapy, observation, and continuous chemotherapy.We searched the PubMed, Embase, and Cochrane databases for relevant articles published through March 2018. All randomized phase-III trials evaluating bevacizumab-based maintenance treatment after bevacizumab-based induction treatment were eligible for inclusion. The primary and secondary endpoints were progression-free survival (PFS) and overall survival (OS), respectively. Hazard ratios (HRs) with 95% confidence intervals (CIs) or data for calculating HRs with 95% CIs were extracted. The RevMan v5.3 (Copenhagen, Denmark) software was used for data analysis.

Results: Nine trials (3121 patients) were included in this meta-analysis. Compared with observation alone, bevacizumab-based maintenance therapy significantly improved PFS (HR: 0.62, 95% CI: 0.47-0.82) and showed a trend toward prolonged OS (HR: 0.93, 95% CI: 0.83-1.05). The incidence of grade 3/4 toxicity, including hypertension and fatigue, was higher after maintenance therapy than after observation alone. PFS (HR: 0.91, 95% CI: 0.70-1.18) and OS (HR: 0.88, 95% CI: 0.74-1.04) did not differ between the maintenance treatment and continuous chemotherapy groups. Grade 3/4 toxicity, including diarrhea and sensory neuropathy, was less common after maintenance therapy than after continuous chemotherapy.

Conclusion: Bevacizumab-based maintenance therapy significantly improved PFS, showed a trend toward prolonged OS, and reduced cumulative grade 3/4 toxicity relative to continuous chemotherapy with comparable efficacy. Although maintenance therapy was beneficial, the optimal strategy should be individualized.
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http://dx.doi.org/10.1097/MD.0000000000018227DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922481PMC
December 2019

MicroRNA-374 targets JAM-2 regulates the growth and metastasis of human pancreatic cancer cells.

Am J Transl Res 2019 15;11(10):6454-6461. Epub 2019 Oct 15.

Department of Pancreatic Surgery, Renmin Hospital of Wuhan University Wuhan 430060, Hubei, China.

Pancreatic cancer is one of the devastating human cancers responsible for tremendous human mortality. Current study was undertaken to explore the therapeutic potential of microRNA-374 in human pancreatic cancer. The results showed that microRNA-374 exhibited lower expression in pancreatic cancer cells and tissues. Overexpression of microRNA-374 in cancer cells resulted in significant decrease in the cell viability. The inhibition of the cell viability was mainly due to the induction of apoptosis as evident from the DAPI and AO/EB staining. Annexin V/PI staining also showed that the overexpression of microRNA-374 enhanced the percentage of apoptotic cells. The western blot analysis showed that microRNA-374 overexpression increases Bax and decreased the Bcl-2 expression. The cleaved PARP and Cleaved caspase-3 expression was also considerably increased upon miR-374 overexpression. The TargetScan analysis together with the dual luciferase assay showed that microRNA-374 targets JAM-2 by binding to mRNA at 3'-UTR. The qRT-PCR analysis showed that JAM-2 was significantly upregulated in the pancreatic cancer cell lines and tissues. Moreover, Overexpression of miR-374 suppressed the expression of JAM-2. Additionally, suppression of JAM-2 also inhibited the viability of the pancreatic cancer cells. studies in xenografted mice models showed that microRNA-374 expression is effective in suppressing the growth and volume of pancreatic tumors. In conclusion, microRNA-374 is downregulated in pancreatic cancers and may exhibit therapeutic implications in the pancreatic cancer treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834505PMC
October 2019

STK11 is required for the normal program of ciliated cell differentiation in airways.

Cell Discov 2019 16;5:36. Epub 2019 Jul 16.

2National Institute of Biological Sciences, 102206 Beijing, China.

The functional properties of mucosal surfaces are dependent on establishing the correct proportions of specialized epithelial cell types. Multiciliated cells (also known as ciliated cells) are evolutionarily conserved and functionally indispensable epithelial cells, as suggested by the link between ciliated cell dysfunction and chronic human disease. Ciliated cell differentiation is an ordered process that involves initial cell fate determination and multiciliogenesis. STK11, a serine/threonine kinase, has been reported to be downregulated in human diseases associated with ciliopathies and functions as a tumor suppressor. Here, we show that STK11 is a physiological factor for the normal program of ciliated cell differentiation by phosphorylating MARK3, which directly suppresses ERK1/2 mediated pRB inactivation. Loss of in airway progenitors impairs the differentiation of ciliated cells in both embryonic and adult airways. Our study establishes that STK11/MARK3/ERK1/2 signaling cascade is a key regulator to integrate ciliated cell fate commitment and the subsequent process of multiciliogenesis.
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http://dx.doi.org/10.1038/s41421-019-0104-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796922PMC
July 2019

Workplace Violence in Chinese Hospitals: The Effects of Healthcare Disturbance on the Psychological Well-Being of Chinese Healthcare Workers.

Int J Environ Res Public Health 2019 09 30;16(19). Epub 2019 Sep 30.

Division of Psychiatry and Applied Psychology, School of Medicine, University of Nottingham, Jubilee Campus, Nottingham NG8 1BB, UK.

Healthcare disturbance is a form of workplace violence against healthcare workers perpetrated by patients, their relatives, and gangs hired by them. It is a prevalent phenomenon in China, where evidence suggests that it impacts on the job satisfaction of healthcare workers. This study aims to examine the relationship between healthcare disturbance, surface acting as a response to emotional labour, and depressive symptoms in Chinese healthcare workers. The study adopted a cross-sectional design and used an online survey methodology. Data were collected from 418 doctors and nurses from one hospital in China. The results showed that frequency of healthcare disturbance was positively related to surface acting and depressive symptoms, respectively; surface acting was also positively related to depression, while deep acting showed no effect on symptoms of depression. Furthermore, surface acting in response to emotional labour mediated the relationship between healthcare disturbance and depressive symptoms. The results highlight the importance of preventing healthcare disturbance and of training healthcare staff in strategies for managing emotional demands in reducing depressive symptoms in Chinese healthcare staff.
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http://dx.doi.org/10.3390/ijerph16193687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801679PMC
September 2019
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