Publications by authors named "Nan Lin"

370 Publications

Substantial leakage into indoor air from on-site solid fuel combustion in chimney stoves.

Environ Pollut 2021 Sep 10;291:118138. Epub 2021 Sep 10.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China; School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.

Exposure to household air pollution (HAP) from solid fuel use (SFU) causes millions of premature deaths globally. Direct leakage from stoves into indoor air is believed to be the main cause of severe HAP. However, previous laboratory-based measurements reported leakage of minimal fractions from wood fuel combustion. Using a newly developed measurement method, on-site measurements were conducted to quantitatively evaluate the leakage of gases and particulate matter from different fuel-stove combinations. The fraction of indoor leakage to the total emission (F) of the measured air pollutants varied from 23 ± 11% to 40 ± 16% for different pollutants and fuel-stove combinations, and these were significantly higher than previously lab-based results. Fuel differences overwhelmed stove differences in influencing F values, with higher values from biomass burning than from coal combustion. The particles had higher F values than gases. Fugitive emission rates (ERs) were log-normally distributed, and biomass burning had higher ERs than coal burning. Indoor PM (fine particulate matter) and CO (carbon monoxide) concentrations measured during the burning period increased by nearly 1-2 orders of magnitude compared to concentrations before or after burning, confirming substantially high indoor leakage from fuel combustion in cookstoves. High fugitive emissions in indoor cookstoves quantified from the present on-site measurements effectively explain the high HAP levels observed in rural SFU households, and call for interventions to improve indoor air quality.
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http://dx.doi.org/10.1016/j.envpol.2021.118138DOI Listing
September 2021

Feminine Hygiene Products and Volatile Organic Compounds in Reproductive-Aged Women Across the Menstrual Cycle: A Longitudinal Pilot Study.

J Womens Health (Larchmt) 2021 Sep 3. Epub 2021 Sep 3.

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, Michigan, USA.

Volatile organic compounds (VOCs) have been detected in feminine hygiene products (FHPs), especially in tampons and sanitary pads. However, little is known about whether menstrual products can contribute to VOC exposure in women. Our objectives were to: (1) examine the variations of urinary VOC concentrations during menstrual cycles; (2) evaluate the relationships between the use of menstrual products and urinary VOC concentrations; and (3) link urinary VOC concentrations to those measured in menstrual products. We measured urinary concentrations of 98 target VOCs in 25 reproductive-aged women with 100 repeated measures collected between October 2018 and February 2019. First-morning-void urine samples were collected four times for each woman during one menstrual cycle. Urinary VOC concentrations were measured using gas chromatography-mass spectroscopy. Of 98 target VOCs measured in the urine samples, 36 VOCs were detected. We did not see statistically significant variations in VOC concentrations across the menstrual cycle. After multivariable adjustment, tampon users had significantly higher concentrations of 2-butanone ( = 1.58 log ng/g, 95% confidence interval [CI]: 0.16-3.00,  = 0.03) and methyl isobutyl ketone ( = 0.63 log ng/g, 95% CI: 0.03-1.22,  = 0.04), compared with pad users. Higher n-nonane, benzene, and toluene estimated from menstrual products were associated with higher urinary concentrations in women. The use of FHPs during menses might be a potential source of VOCs. A larger cohort study is warranted to confirm our results and evaluate clinical implications.
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http://dx.doi.org/10.1089/jwh.2021.0153DOI Listing
September 2021

Oncogenic PAX6 elicits CDK4/6 inhibitor resistance by epigenetically inactivating the LATS2-Hippo signaling pathway.

Clin Transl Med 2021 Aug;11(8):e503

Department of Gastroenterology and Hepatology, the First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou, 510630, China.

Intrinsic resistance to CDK4/6 inhibitors hinders their clinical utility in cancer treatment. Furthermore, the predictive markers of CDK4/6 inhibitors in gastric cancer (GC) remain incompletely described. Here, we found that PAX6 expression was negatively correlated with the response to palbociclib in vitro and in vivo in GC. We observed that the PAX6 expression level was negatively correlated with the overall survival of GC patients and further showed that PAX6 can promote GC cell proliferation and the cell cycle. The cell cycle is regulated by the interaction of cyclins with their partner serine/threonine cyclin-dependent kinases (CDKs), and the G1/S-phase transition is the main target of CDK4/6 inhibitors. Therefore, we tested whether PAX6 expression was correlated with the GC response to palbociclib. We found that PAX6 hypermethylates the promoter of LATS2 and inactivates the Hippo pathway, which upregulates cyclin D1 (CCND1) expression. This results in a suppressed response to palbociclib in GC. Furthermore, we found that the induction of the Hippo signaling pathway or treatment with a DNA methylation inhibitor could overcome PAX6-induced palbociclib resistance in GC. These findings uncover a tumor promoter function of PAX6 in GC and establish overexpressed PAX6 as a mechanism of resistance to palbociclib.
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http://dx.doi.org/10.1002/ctm2.503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8382979PMC
August 2021

Prevalence of SARS-CoV-2 IgG antibodies in health care workers at a tertiary care academic medical center - An assessment of occupational infection risk.

Am J Infect Control 2021 09;49(9):1158-1161

College of Medicine, University of Kentucky, Lexington, KY; College of Pharmacy, University of Kentucky, Lexington, KY; Markey Cancer Center, University of Kentucky, Lexington, KY. Electronic address:

Background: The purpose of this study was to assess the occupational SARS-CoV-2 infection risk among health care workers (HCW) at University of Kentucky HealthCare (UKHC) by evaluating the prevalence of SARS-CoV-2 antibodies.

Methods: This is a prospective cohort study of HCW at UKHC. SARS-CoV-2 IgG antibody seropositivity was measured in a CLIA-certified laboratory utilizing the Abbott Architect SARS-CoV-2 IgG antibody assay. Demographics and work type were self-reported by study participants via an emailed survey.

Results: The overall antibody positivity rate of HCW was 1.55% (5/322; 95% confidence interval: 0.65%-3.71%) at cohort entry. There were no differences in antibody positivity between those that worked directly with SARS-CoV-2 infected patients and those that did not. The antibody rate of positivity of patients during the same time period was similar, 1.8% (9/499; 95% confidence interval 0.94%-3.45%).

Conclusions: Antibody positivity was low and similar between HCW and patients tested during a similar time period. HCW positivity rates did not appear to be impacted by caring for known SARS-CoV-2 infected patients suggesting that appropriate use of personal protective equipment is effective in protecting individuals from transmission.
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http://dx.doi.org/10.1016/j.ajic.2021.04.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387089PMC
September 2021

Dispositional Awe and Online Altruism: Testing a Moderated Mediating Model.

Front Psychol 2021 4;12:688591. Epub 2021 Aug 4.

School of Psychology, Fujian Normal University, Fuzhou, China.

Dispositional awe has a positive effect on prosociality. However, it has not been tested whether this disposition appears in online altruism. Using a large sample of 3,080 Chinese undergraduates, this study tested a moderated mediating model that takes self-transcendent meaning in life (STML) as a mediator and subjective socioeconomic status (SSES) as a moderator. As predicted, dispositional awe was positively correlated with online altruism, partly the indirect effect of STML. SSES moderated both the direct and indirect effects. Specifically, the predictive effects of dispositional awe on both online prosocial behavior and STML were greater for lower rather than higher SSES. This study extends the prosociality of dispositional awe to cyberspace. Other implications are also discussed herein.
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http://dx.doi.org/10.3389/fpsyg.2021.688591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371932PMC
August 2021

Differences in Multimodal Electroencephalogram and Clinical Correlations Between Early-Onset Alzheimer's Disease and Frontotemporal Dementia.

Front Neurosci 2021 5;15:687053. Epub 2021 Aug 5.

Department of Neurology, Peking Union Medical College Hospital, Beijing, China.

Background: Alzheimer's disease (AD) and frontotemporal dementia (FTD) are the two main types of dementia. We investigated the electroencephalogram (EEG) difference and clinical correlation in early-onset Alzheimer's disease (EOAD), and FTD using multimodal EEG analyses. EOAD had more severe EEG abnormalities than late-onset AD (LOAD). Group comparisons between EOAD and LOAD were also performed.

Methods: Thirty patients diagnosed with EOAD, nine patients with LOAD, and 14 patients with FTD (≤65 y) were recruited (2008.1-2020.2), along with 24 healthy controls (≤65 y, = 18; >65 y, = 6). Clinical data were reviewed. Visual EEG, EEG microstate, and spectral analyses were performed.

Results: Compared to controls, markedly increased mean microstate duration, reduced mean occurrence, and reduced global field power (GFP) peaks per second were observed in EOAD and FTD. We found increased durations of class B in EOAD and class A in FTD. EOAD had reduced occurrences in classes A, B, and C, while only class C occurrence was reduced in FTD. The visual EEG results did not differ between AD and FTD. Microstate B showed correlations with activities of daily living score ( = 0.780, = 0.008) and cerebrospinal fluid (CSF) Aβ42 ( = -0.833, = 0.010) in EOAD. Microstate D occurrence was correlated with the CSF Aβ42 level in FTD ( = 0.786, = 0.021). Spectral analysis revealed a general slowing EEG, which may contribute to microstate dynamic loss. Power in delta was significantly higher in EOAD than in FTD all over the head. In addition, EOAD had a marked increased duration and decreased occurrence than late-onset AD (LOAD), with no group differences in visual EEG results.

Conclusion: The current study found that EOAD and FTD had different EEG changes, and microstate had an association with clinical severity and CSF biomarkers. EEG microstate is more sensitive than visual EEG and may be useful for the differentiation between AD and FTD. The observations support that EEG can be a potential biomarker for the diagnosis and assessment of early-onset dementias.
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http://dx.doi.org/10.3389/fnins.2021.687053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8374312PMC
August 2021

Self-Healing, Self-Adhesive Silk Fibroin Conductive Hydrogel as a Flexible Strain Sensor.

ACS Appl Mater Interfaces 2021 Aug 10;13(33):40013-40031. Epub 2021 Aug 10.

College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215100, China.

Flexible and wearable hydrogel strain sensors have attracted tremendous attention for applications in human motion and physiological signal monitoring. However, it is still a great challenge to develop a hydrogel strain sensor with certain mechanical properties and tensile deformation capabilities, which can be in conformal contact with the target organ and also have self-healing properties, self-adhesive capability, biocompatibility, antibacterial properties, high strain sensitivity, and stable electrical performance. In this paper, an ionic conductive hydrogel (named PBST) is rationally designed by proportionally mixing polyvinyl alcohol (PVA), borax, silk fibroin (SF), and tannic acid (TA). SF can not only be a reinforcement to introduce an energy dissipation mechanism into the dynamically cross-linked hydrogel network to stabilize the non-Newtonian behavior of PVA and borax but it can also act as a cross-linking agent to combine with TA to reduce the dissociation of TA on the hydrogel network, improving the mechanical properties and viscoelasticity of the hydrogel. The combination of SF and TA can improve the self-healing ability of the hydrogel and realize the adjustable viscoelasticity of the hydrogel without sacrificing other properties. The obtained hydrogel has excellent stretchability (strain > 1000%) and shows good conformal contact with human skin. When the hydrogel is damaged by external strain, it can rapidly self-repair (mechanical and electrical properties) without external stimuli. It shows adhesiveness and repeatable adhesiveness to different materials (steel, wood, PTFE, glass, iron, and cotton fabric) and biological tissues (pigskin) and is easy to peel off without residue. The obtained PBST conductive hydrogel also has a wide strain-sensing range (>650%) and reliable stability. The hydrogel adhered to the skin surface can monitor large strain movements such as in finger joints, wrist joints, knee joints, and so on and detect swallowing, smiling, facial bulging and calming, and other micro-deformation behaviors. It can also distinguish physical signals such as light smile, big laugh, fast and slow breathing, and deep and shallow breathing. Therefore, the PBST conductive hydrogel material with multiple synergistic functions has great potential as a flexible wearable strain sensor. The PBST hydrogel has antibacterial properties and good biocompatibility at the same time, which provides a safety guarantee for it as a flexible wearable strain sensor. This work is expected to provide a new way for people to develop ideal wearable strain sensors.
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http://dx.doi.org/10.1021/acsami.1c08395DOI Listing
August 2021

Lapatinib and poziotinib overcome ABCB1-mediated paclitaxel resistance in ovarian cancer.

PLoS One 2021 4;16(8):e0254205. Epub 2021 Aug 4.

Markey Cancer Center, College of Medicine, University of Kentucky, Lexington, KY, United States of America.

Conventional frontline treatment for ovarian cancer consists of successive chemotherapy cycles of paclitaxel and platinum. Despite the initial favorable responses for most patients, chemotherapy resistance frequently leads to recurrent or refractory disease. New treatment strategies that circumvent or prevent mechanisms of resistance are needed to improve ovarian cancer therapy. We established in vitro paclitaxel-resistant ovarian cancer cell line and organoid models. Gene expression differences in resistant and sensitive lines were analyzed by RNA sequencing. We manipulated candidate genes associated with paclitaxel resistance using siRNA or small molecule inhibitors, and then screened the cells for paclitaxel sensitivity using cell viability assays. We used the Bliss independence model to evaluate the anti-proliferative synergy for drug combinations. ABCB1 expression was upregulated in paclitaxel-resistant TOV-21G (q < 1x10-300), OVCAR3 (q = 7.4x10-156) and novel ovarian tumor organoid (p = 2.4x10-4) models. Previous reports have shown some tyrosine kinase inhibitors can inhibit ABCB1 function. We tested a panel of tyrosine kinase inhibitors for the ability to sensitize resistant ABCB1-overexpressing ovarian cancer cell lines to paclitaxel. We observed synergy when we combined poziotinib or lapatinib with paclitaxel in resistant TOV-21G and OVCAR3 cells. Silencing ABCB1 expression in paclitaxel-resistant TOV-21G and OVCAR3 cells reduced paclitaxel IC50 by 20.7 and 6.2-fold, respectively. Furthermore, we demonstrated direct inhibition of paclitaxel-induced ABCB1 transporter activity by both lapatinib and poziotinib. In conclusion, lapatinib and poziotinib combined with paclitaxel synergizes to inhibit the proliferation of ABCB1-overexpressing ovarian cancer cells in vitro. The addition of FDA-approved lapatinib to second-line paclitaxel therapy is a promising strategy for patients with recurrent ovarian cancer.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0254205PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336885PMC
August 2021

Occurrence and distribution of per- and polyfluoroalkyl substances (PFASs) in human livers with liver cancer.

Environ Res 2021 Jul 29;202:111775. Epub 2021 Jul 29.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China.

Per- and polyfluoroalkyl substances (PFASs) are anthropogenic compounds that are widely accumulated in human tissues, and the liver is considered a primary target organ for PFASs exposure. The occurrence and distribution of 21 PFASs in liver tissues with tumors (n = 55) and without tumors (n = 55) are investigated in this study. Eleven perfluorinated carboxylic acids (PFCAs) and five perfluorinated sulfonic acids (PFSAs) were detected at high frequencies (45.5%-100 %), while the detection frequencies of five perfluoroalkyl phosphate (PFPAs) were relatively lower (≤29.1 %). PFSAs and PFCAs accounted for up to 82.5%-92.7 % of the total PFASs. Although it was not found to be statistically significant, the concentrations of the total PFASs were slightly higher in the tumor liver samples (mean 64.3, range 5.70-303 ng/g) than those in the non-tumor liver samples (mean 62.7, range 4.08-240 ng/g).The perfluorooctanoic acid (PFOA), perfluorotridecanoic acid (PFTrDA), and perfluorobutanesulphonate (PFBS) showed significant differences (p < 0.05) between the tumor and non-tumor liver samples, and the different distribution levels of these three PFASs may have been a consequence of oxidative stress. The total concentrations of PFASs in the three age groups were in the decreasing order of middle-aged people (45-60) > old people (>60) > young people (<45). The PFASs in females were generally lower than in males, which may have been related to women's special excretion methods (such as childbirth and breastfeeding). The results should be valuable for further mechanistic studies regarding the toxic effects of PFASs in human livers.
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http://dx.doi.org/10.1016/j.envres.2021.111775DOI Listing
July 2021

Field-based measurements of major air pollutant emissions from typical porcelain kiln in China.

Environ Pollut 2021 Jul 19;288:117810. Epub 2021 Jul 19.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China.

China has been famous for its porcelains for millennia, and the combustion processes of porcelain production emit substantial amounts of air pollutants, which have not been well understood. This study provided firsthand data of air pollutant emissions from biomass porcelain kilns. The emission factor of PM was 0.95 ± 1.23 g/kg during the entire combustion cycle, lower than that of biomass burning in residential stoves and coal burning in brick kilns, attributed to the removal effects of the long-distance transport in dragon kilns. The temporal trend of particle pollutants, including particulate matters (PMs) and particulate polycyclic aromatic hydrocarbons (PAHs) (low at ignition phase and high at the end) again indicated the removal effects of the special structure, while gaseous pollutants, such as gaseous PAHs, exhibited the opposite result. The GWC was estimated as 1.4 × 10 and 0.5 × 10 kg COe/yr for the scenarios in which 50% and 100% of the wood was renewable, respectively. The GWC of dragon kilns is nearly equal to that of 745 households using wood-fueled stoves. These results indicate the necessity of pollution controls for biomass porcelain kilns to estimate the emission inventory and climate change.
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http://dx.doi.org/10.1016/j.envpol.2021.117810DOI Listing
July 2021

Tripchlorolide attenuates β-amyloid generation by inducing NEP activity in N2a/APP695 cells.

Transl Neurosci 2021 Jan 20;12(1):301-308. Epub 2021 Jul 20.

Department of Neurology, Institute of Clinical Neurology, Fujian Medical University Union Hospital, 29 Xinquan Road, Fuzhou, Fujian, 350001, China.

Background And Purpose: Alzheimer's disease (AD) is a neurodegeneration disease. The previous work from our research group demonstrated the neuroprotective effects of tripchlorolide (T4) in AD animal models.

Materials And Methods: Neprilysin (NEP) is known as an important physiological amyloid-β protein (Aβ) peptide-degrading enzyme in the brain due to its apparent rate-limiting function. In this study, we explored the effect of NEP on AD model N2a/APP695 cells. Western blots and enzyme-linked immunosorbent assays were performed to assess the expression of proteins, while quantitative real-time polymerase chain reaction assays were used to evaluate RNA levels. Cell vitality was detected by the MTT assay, and reactive oxygen species (ROS) levels were assessed using a ROS activity assay kit.

Results: We discovered that T4 was able to enhance the enzyme activity of NEP. T4 administration decreased the protein levels of the soluble amyloid precursor protein. In further experiments, we found that by using thiorphan the secretion of Aβ, oxidative stress, nitrosative stress, and inflammatory factors, which were suppressed by T4, were reversed. Due to its ability to attenuate Aβ generation and to protect neurons against the neurotoxicity of Aβ, T4 may be a potential therapy in the regulation of Aβ-related pathology in AD by affecting NEP activity.

Conclusion: Tripchlorolide attenuates Aβ generation by inducing NEP activity in N2a/APP695 cells.
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http://dx.doi.org/10.1515/tnsci-2020-0178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8294110PMC
January 2021

The efficacy of immune checkpoint inhibitors in advanced hepatocellular carcinoma: a meta-analysis based on 40 cohorts incorporating 3697 individuals.

J Cancer Res Clin Oncol 2021 Jul 23. Epub 2021 Jul 23.

Department of Oncology, Molecular Oncology Research Institute, The First Affiliated Hospital, Fujian Medical University, Chazhong Road No. 20, Fuzhou, 350005, Fujian, China.

Background: This study was designed to investigate the efficacy and safety of immune checkpoint inhibitors (ICIs) in advanced hepatocellular carcinoma (HCC).

Methods: Electronic databases were scanned to identify relevant trials. The primary endpoints were overall survival (OS), progression-free survival (PFS), and their prognostic factors. Stratified analyses were accomplished on ICIs agent and evaluation criteria.

Results: Totally, 3697 individuals from 40 cohorts were recruited. For patients treated with ICIs, the pooled median time to progression (TTP) was 8.0 months, median PFS 4.9 months, and median OS 12.0 months; the pooled median PFS and OS of ICIs plus anti-vascular endothelial growth factor (VEGF) agents (PFS: 6.3 months, OS: 16.4 months) were longer than those of ICIs alone. Furthermore, Child-Pugh stage (HR = 1.37, P = 0.0123) and Eastern Cooperative Oncology Group (ECOG) (HR = 1.40, P = 0.0016) were prognostic factors for PFS. Hepatitis C virus (HCV) (HR = 0.71, P = 0.0356), Alpha-fetoprotein (AFP) (HR = 1.17, P < 0.0001), Child-Pugh stage (HR = 1.58, P < 0.0001), Barcelona Clinic Liver Cancer (BCLC) stage (HR = 1.23, P = 0.0005), ECOG (HR = 1.50, P = 0.0012), portal vein invasion (HR = 1.32, P = 0.0053), extrahepatic metastasis (HR = 0.84, P = 0.0047), best response (HR = 0.58, P < 0.0001), and neutrophil-to-lymphocyte ratio (NLR) (HR = 1.23, P = 0.0451) were the prognostic factors for OS. According to both RECIST 1.1 and mRECIST, the objective response rate (ORR) and disease control rate (DCR) rate of ICIs plus anti-VEGF agents were better than those of ICIs alone. The overall rate of any grade adverse events (AEs) was 0.76 (95% CI 0.61-0.89), grade 3 or higher AEs was 0.28 (95% CI 0.15-0.42), and the rate of AEs leading to treatment discontinuation was 0.09 (95% CI 0.06-0.12).

Conclusions: The ICIs was promising in HCC with good efficacy and tolerated toxicity. Compared with ICIs monotherapy, the joint application of ICIs and anti-VEGF agents can contribute a lot more benefits to the survival of patients according to clinical practices.
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http://dx.doi.org/10.1007/s00432-021-03716-1DOI Listing
July 2021

miR-383-5p inhibits human malignant melanoma cells function via targeting CENPF.

Reprod Biol 2021 Sep 16;21(3):100535. Epub 2021 Jul 16.

Department of Hand and Plastic Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, No.109 Xueyuan Western Road, Wenzhou, Zhejiang, 325027, PR China. Electronic address:

Human malignant melanoma (MM), is a type of skin cancer with high morbidity and mortality. In this study, we investigated the role of miR-383-5p in human MM cells in vitro. miR-383-5p expression was downregulated in MM cell lines compared with the human normal melanocyte cell line, and miR-383-5p overexpression inhibited the proliferation, migration, and invasion of M14 and A375 cells. Furthermore, miR-383-5p was able to effectively bind to the 3'UTR of CENPF mRNA. miR-383-5p expression was negatively correlated with CENPF expression and miR-383-5p overexpression inhibited CENPF protein expression in M14 and A375 cells. The overexpression of CENPF could effectively rescue the inhibitory effect on proliferation and invasion caused by miR-383-5p. Additionally, using publicly available databases, we showed that CENPF expression was upregulated in human MM tissues and could predict the prognosis of MM. In conclusion, miR-383-5p acts as a tumor suppressor in human MM by targeting CENPF, suggesting CENPF as a potential therapeutic target for human MM.
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http://dx.doi.org/10.1016/j.repbio.2021.100535DOI Listing
September 2021

Long-term seizure outcomes in patients with anti-Leucine-rich glioma-inactivated 1 encephalitis.

Epilepsy Behav 2021 09 3;122:108159. Epub 2021 Jul 3.

Department of Neurology, Peking Union Medical College Hospital, China. Electronic address:

Background: This study aimed to investigate the semiology of seizure disorders, including electroencephalographic characteristics, and seizure outcomes in participants with anti-leucine-rich glioma-inactivated 1 (LGI-1) encephalitis.

Methods: Seventy participants who presented with seizures during the acute phase of anti-LGI-1 encephalitis at Peking Union Medical College Hospital from May 2013 to July 2020 were reviewed. All participants underwent follow-up for longer than 2 years.

Results: At the time of presentation, 48 (68.6%) participants had generalized seizures and 57 (81.4%) had focal seizures. The most common focal motor seizures were faciobrachial dystonic seizures (FDS). The main manifestations of focal nonmotor seizures were dyscognitive features, goosebumps, and disorders of sensation. All participants received immunomodulatory therapy. Thirty-five (50%) participants were seizure free after 1 year of follow-up, and 48 (68.6%) participants were seizure free over a follow-up of 2 years. Participants with seizures continued longer than 1 year were older than participants whose seizure duration was shorter than 1 year (P = 0.021). However, after an extended follow-up period, the difference between the incidences of seizures based on age was not significant. The frequency of focal motor seizures was higher in participants who became seizure free within 1 year, compared to participants who had seizures for longer than 1 year (75% vs 54.3%, respectively; P = 0.015). Participants with seizures continued over 2 years tended to have focal nonmotor seizures, and tended to show an elevated incidence of abnormal EEG results. Participants receiving early corticosteroid and longer duration immunosuppressant treatments, tended to have a lower risk of persistent seizures and better seizure outcomes, with no statistical significance.

Conclusions: Most participants obtained remission from seizures after immunomodulatory therapy. The seizure manifestation of anti-LGI1 encephalitis is diverse and variable. The type of focal seizures may affect the outcome of participants with seizures. Older age could lead to longer duration of the seizure disorder, but did not affect the rate of seizures over the long term. Early and prolonged administration of immunomodulatory therapy may be useful for shortening the time to becoming seizure free.
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http://dx.doi.org/10.1016/j.yebeh.2021.108159DOI Listing
September 2021

Silk sericin/fibroin electrospinning dressings: a method for preparing a dressing material with high moisture vapor transmission rate.

Authors:
Nan Lin Baoqi Zuo

J Biomater Sci Polym Ed 2021 Jul 28:1-15. Epub 2021 Jul 28.

National Engineering Laboratory for Modern Silk, Suzhou, China.

The current study focuses on the preparation of sericin and silk fibroin blend electrostatic spinning fiber film dressing. The surface morphology of the fiber films was observed by scanning electron microscope, and the hydrophilicity and swelling property of the fiber membrane dressing were analyzed. The biocompatibility of the four dressings was verified by the CCK-8 method and confocal laser microscopy. This experiment showed that the dressing group with the ratio of sericin to silk fibroin of 3:7 had better performance, offering fine and uniform fiber structure, good surface hydrophilicity, high water vapor transmission rate. The swelling rate of it was 822.77 ± 62.78%, and the tensile properties reached the requirements of dressing materials and had an excellent ability to promote cell adhesion and proliferation. This paper provides a possible method for producing of dressing materials with good hydrophilicity and high moisture vapor transmission rate.
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http://dx.doi.org/10.1080/09205063.2021.1952383DOI Listing
July 2021

Sequencing of 640,000 exomes identifies variants associated with protection from obesity.

Science 2021 07;373(6550)

Geisinger Obesity Institute, Geisinger Health System, Danville, PA 17882, USA.

Large-scale human exome sequencing can identify rare protein-coding variants with a large impact on complex traits such as body adiposity. We sequenced the exomes of 645,626 individuals from the United Kingdom, the United States, and Mexico and estimated associations of rare coding variants with body mass index (BMI). We identified 16 genes with an exome-wide significant association with BMI, including those encoding five brain-expressed G protein-coupled receptors (, , , , and ). Protein-truncating variants in were observed in ~4/10,000 sequenced individuals and were associated with 1.8 kilograms per square meter lower BMI and 54% lower odds of obesity in the heterozygous state. Knock out of in mice resulted in resistance to weight gain and improved glycemic control in a high-fat diet model. Inhibition of GPR75 may provide a therapeutic strategy for obesity.
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http://dx.doi.org/10.1126/science.abf8683DOI Listing
July 2021

Demographic history and local adaptation of (Asteraceae) provide insight on plant evolution in northern China flora.

Ecol Evol 2021 Jun 17;11(12):8000-8013. Epub 2021 May 17.

CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany Chinese Academy of Sciences Kunming China.

The flora of northern China forms the main part of the Sino-Japanese floristic region and is located in a south-north vegetative transect in East Asia. Phylogeographic studies have demonstrated that an arid belt in this region has promoted divergence of plants in East Asia. However, little is known about how plants that are restricted to the arid belt of flora in northern China respond to climatic oscillation and environmental change. Here, we used genomic-level data of across its distribution as a representative of northern China flora to reconstruct plant demographic history, examine local adaptation related to environmental disequilibrium, and investigate the factors related to effective population size change. Our results indicate originated from the northern area and expanded to the southern area, with the Taihang Mountains serving as a physical barrier promoting population divergence. Genome-wide evidence found strong correlation between genomic variation and environmental factors, specifically signatures associated with local adaptation to drought stress in heterogeneous environments. Multiple linear regression analyses revealed joint effects of population age, mean temperature of coldest quarter, and precipitation of wettest month on effective population size (). Our current study uses as a case for providing new insights into the evolutionary history and local adaptation of northern China flora and provides qualitative strategies for plant conservation.
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http://dx.doi.org/10.1002/ece3.7628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216978PMC
June 2021

Airborne volatile organic compounds at an e-waste site in Ghana: Source apportionment, exposure and health risks.

J Hazard Mater 2021 10 10;419:126353. Epub 2021 Jun 10.

Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI, USA 48109. Electronic address:

Informal e-waste recycling processes emit various air pollutants. While there are a number of pollutants of concern, little information exists on volatile organic compounds (VOCs) releases at e-waste sites. To assess occupational exposures and estimate health risks, we measured VOC levels at the Agbogbloshie e-waste site in Ghana, the largest e-waste site in Africa, by collecting both fixed-site and personal samples for analyzing a wide range of VOCs. A total of 54 VOCs were detected, dominated by aliphatic and aromatic compounds. Mean and median concentrations of the total target VOCs were 46 and 37 μg/m at the fixed sites, and 485 and 162 μg/m for the personal samples. Mean and median hazard ratios were 2.1 and 1.4, respectively, and cancer risks were 4.6 × 10 and 1.5 × 10. These risks were predominantly driven by naphthalene and benzene; chloroform and formaldehyde were also high in some samples. Based on the VOC composition, the major sources were industry, fuel evaporation and combustion. The concentration gradient across sites and the similarity of VOC profiles indicated that the e-waste site emissions reached neighboring communities. Our results suggest the need to protect e-waste workers from VOC exposure, and to limit emissions that can expose nearby populations.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126353DOI Listing
October 2021

Prognostic value of PTEN in diagnosed metastatic prostate cancer.

Asian J Androl 2021 May 28. Epub 2021 May 28.

Department of Urology, Fudan University Shanghai Cancer Center, Shanghai 200032, China.

The purpose of our study is to investigate the prognostic value of phosphatase and tensin homolog on chromosome 10 (PTEN) expression in patients with de novo metastatic castration naïve prostate cancer (mCNPC). A total of 205 patients with mCNPC at Fudan University Shanghai Cancer Center (Shanghai, China) were retrospectively examined. Immunohistochemical staining of PTEN was performed on prostate biopsy samples of these patients. Associations among clinicopathological features, patient survival and PTEN protein expression were analyzed. PTEN loss occurred in 58 of 205 (28.3%) patients. Loss of PTEN was significantly correlated with high metastatic volume (P = 0.017). No association between PTEN expression and Gleason score was observed. Patients with PTEN loss had significantly shorter progression-free survival (PFS, P < 0.001) and overall survival (OS, P < 0.001) compared with patients with intact PTEN expression. Multivariate analysis showed that elevated alkaline phosphatase, high metastatic volume and PTEN loss were independent poor prognostic factors for PFS. The Eastern Cooperative Oncology Group performance status (ECOG PS) ≥2 and PTEN loss were independent poor prognostic factors for OS. The adjusted hazard ratio of PTEN loss for PFS and OS was 1.67 (95% confidence interval [CI]: 1.14-2.43, P = 0.008) and 1.95 (95% CI: 1.23-3.10, P = 0.005), respectively. PTEN loss was also significantly associated with shorter PFS (P = 0.025) and OS (P < 0.001) in patients with low-volume metastatic disease. Our data showed that PTEN loss is an independent predictor for shorter PFS and OS in patients with de novo mCNPC.
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http://dx.doi.org/10.4103/aja.aja_39_21DOI Listing
May 2021

Dexmedetomidine inhibits unstable motor network in patients with primary motor area gliomas.

Aging (Albany NY) 2021 05 25;13(11):15139-15150. Epub 2021 May 25.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China.

Background: Sedative agents such as dexmedetomidine have been found to transiently exacerbate or unmask limb motor dysfunction in patients with eloquent area brain gliomas. The present study aims to investigate whether dexmedetomidine can inhibit motor plasticity in patients with glioma via fMRI.

Methods: 21 patients with brain glioma were prospectively recruited between September 2017 and December 2018. Patients were classified into pre-M1 (primary motor cortex) group (n=9), post-M1 group (n=6), and non-eloquent group (control group) (n=6) according to the tumor position related to M1. The hand movement task-fMRI and resting state fMRI (rs-fMRI) were performed before and after sedation using dexmedetomidine. The lateralization index (LI) of activation voxels and magnitude and the functional connectivity (FC) of motor network were compared before and after sedation and among different groups.

Results: Permanent postoperative motor deficit of the upper limb was found in 5 of 6 patients in the pre-M1 group, and none in other groups ( < .01). Task-fMRI showed the LI of activation volume and activation magnitude at M1 significantly increased only in the pre-M1 group after sedation ( < .05). Rs-fMRI showed 60.0% (27 of 45) FCs of motor network decreased in pre-M1 group after sedation (p[FDR] < .05); whereas there was no FC reduction in post-M1 and control groups (p[FDR] > .05).

Conclusions: In patients with eloquent area gliomas, dexmedetomidine can inhibit the unstable compensative motor plasticity on both task- and rs-fMRI. fMRI may be a promising method for elucidating the effect of sedative agents on motor plasticity.
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http://dx.doi.org/10.18632/aging.203077DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221338PMC
May 2021

Application of nano-carbon and titanium clip combined labeling in robot-assisted laparoscopic transverse colon cancer surgery.

BMC Surg 2021 May 24;21(1):257. Epub 2021 May 24.

Department of General Surgery, 900 Hospital of the Joint Logistics Team, Fuzhou, China.

Background: Robot-assisted laparoscopic transverse colon tumor surgery requires precise tumor localization. The purpose of this study was to evaluate the safety and efficacy of nano-carbon and titanium clip combination labeling methods in robot-assisted transverse colon tumor surgery.

Methods: From January 2018 to January 2019, the clinical data of 16 patients who come from FuZhou, China underwent preoperative nano-carbon and titanium clip combined with robot-assisted laparoscopic transverse colon cancer surgery were retrospectively analyzed.

Results: Of the 16 patients, no signs of abdominal pain, fever, or diarrhea were observed after colonoscopy. Two titanium clips were seen on all of the 16 patients' abdominal plain films. Nano-carbon staining sites were observed during the operation, and no staining disappeared or abdominal cavity contamination. All patients underwent R resection. The average number of lymph nodes harvsted was 18.23 ± 5.04 (range, 9-32). The average time to locate the lesion under the laparoscopic was 3.03 ± 1.26 min (range, 1-6 min), and the average operation time was 321.43 ± 49.23 min (range, 240-400 min). All were consistent with the surgical plan, and there was no intraoperative change of surgical procedure or conversion to open surgery.

Conclusion: Preoperative colonoscopy combined with nano-carbon and titanium clip is safe and effective in robot-assisted transverse colon cancer surgery. A At the same time, the labeling method shows potential in shortening the operation time, ensuring sufficient safety margin and reducing complications.
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http://dx.doi.org/10.1186/s12893-021-01248-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142471PMC
May 2021

Development and validation of a postoperative delirium prediction model for pediatric patients: A prospective, observational, single-center study.

Medicine (Baltimore) 2021 May;100(20):e25894

Nursing Department.

Abstract: Postoperative delirium is a serious complication that relates to poor outcomes. A risk prediction model could help the staff screen for children at high risk for postoperative delirium. Our study aimed to establish a postoperative delirium prediction model for pediatric patients and to verify the sensitivity and specificity of this model.Data were collected from a total of 1134 children (0-16yr) after major elective surgery between February 2020 to June 2020. Demographic and clinical data were collected to explore the risk factors. Multivariate logistic regression analysis was used to develop the model, and we assessed the predictive ability of the model by using the area under the receiver operating characteristics curve (AUROC). Further data were collected from another 100 patients in October 2020 to validate the model.Prevalence of postoperative delirium in this sample was 11.1%. The model consisted of 5 predictors, namely, age, developmental delay, type of surgery, pain, and dexmedetomidine. The AUROC was 0.889 (P < .001, 95% confidence interval (CI):0.857-0.921), with sensitivity and specificity of 0.754 and 0.867, and the Youden of 0.621. The model verification results showed the sensitivity of 0.667, the specificity of 0.955.Children undergoing surgery are at risk for developing delirium during the postoperative period, young age, developmental delay, otorhinolaryngology surgery, pain, and exposure to dexmedetomidine were associated with increased odds of delirium. Our study established a postoperative delirium prediction model for pediatric patients, which may be a base for development of strategies to prevent and treat postoperative delirium in children.
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http://dx.doi.org/10.1097/MD.0000000000025894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137008PMC
May 2021

Recovery of consciousness and cognition after general anesthesia in humans.

Elife 2021 05 10;10. Epub 2021 May 10.

Department of Anesthesiology and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.

Understanding how the brain recovers from unconsciousness can inform neurobiological theories of consciousness and guide clinical investigation. To address this question, we conducted a multicenter study of 60 healthy humans, half of whom received general anesthesia for 3 hr and half of whom served as awake controls. We administered a battery of neurocognitive tests and recorded electroencephalography to assess cortical dynamics. We hypothesized that recovery of consciousness and cognition is an extended process, with differential recovery of cognitive functions that would commence with return of responsiveness and end with return of executive function, mediated by prefrontal cortex. We found that, just prior to the recovery of consciousness, frontal-parietal dynamics returned to baseline. Consistent with our hypothesis, cognitive reconstitution after anesthesia evolved over time. Contrary to our hypothesis, executive function returned first. Early engagement of prefrontal cortex in recovery of consciousness and cognition is consistent with global neuronal workspace theory.
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http://dx.doi.org/10.7554/eLife.59525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163502PMC
May 2021

The Effects of Inter-Set Recovery Time on Explosive Power, Electromyography Activity, and Tissue Oxygenation during Plyometric Training.

Sensors (Basel) 2021 Apr 25;21(9). Epub 2021 Apr 25.

School of Biomedical Engineering, Dalian University of Technology, Dalian 116024, China.

Performing continuous sets to failure is fatiguing during the plyometric training. Cluster sets have been used to redistribute total rest time to create short frequent sets so that muscle fatigue can be avoided. The purpose of the study was to investigate the effects of inter-set recovery time on lower extremity explosive power, neuromuscular activity, and tissue oxygenation during plyometric exercise and recovery. An integrated assessment of explosive power, muscle electrical activity, and tissue oxygenation was adopted in the present study to help understand local muscle metabolism and fatigue during plyometric exercise and recovery. Ten university male basketball players participated in this study. Subjects performed 4 groups of exercise, each group comprised of 3 sets of jumps: 1, 2, 3, or 5 min. Surface electromyography (sEMG) signals were collected from 9 lower extremity muscles; near-infrared spectroscopy (NIRS) was recorded on ; mechanical data during plyometric exercise were collected from a force plate. No significant differences among sets and among groups were found regarding explosive power, jump height, EMG intensity, mean power frequency, the rate of tissue saturation index, and HbO changes between baseline and recovery. The current study has shown no muscular fatigue induced during the 4 groups of exercise. The results of this study may help inform recommendations concerning the recovery time during plyometric exercises at low loads (30% 1 RM).
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http://dx.doi.org/10.3390/s21093015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123263PMC
April 2021

Seg-CapNet: A Capsule-Based Neural Network for the Segmentation of Left Ventricle from Cardiac Magnetic Resonance Imaging.

J Comput Sci Technol 2021 31;36(2):323-333. Epub 2021 Mar 31.

School of Software, Zhengzhou University, Zhengzhou, 450000 China.

Deep neural networks (DNNs) have been extensively studied in medical image segmentation. However, existing DNNs often need to train shape models for each object to be segmented, which may yield results that violate cardiac anatomical structure when segmenting cardiac magnetic resonance imaging (MRI). In this paper, we propose a capsule-based neural network, named Seg-CapNet, to model multiple regions simultaneously within a single training process. The Seg-CapNet model consists of the encoder and the decoder. The encoder transforms the input image into feature vectors that represent objects to be segmented by convolutional layers, capsule layers, and fully-connected layers. And the decoder transforms the feature vectors into segmentation masks by up-sampling. Feature maps of each down-sampling layer in the encoder are connected to the corresponding up-sampling layers, which are conducive to the backpropagation of the model. The output vectors of Seg-CapNet contain low-level image features such as grayscale and texture, as well as semantic features including the position and size of the objects, which is beneficial for improving the segmentation accuracy. The proposed model is validated on the open dataset of the Automated Cardiac Diagnosis Challenge 2017 (ACDC 2017) and the Sunnybrook Cardiac Magnetic Resonance Imaging (MRI) segmentation challenge. Experimental results show that the mean Dice coefficient of Seg-CapNet is increased by 4.7% and the average Hausdorff distance is reduced by 22%. The proposed model also reduces the model parameters and improves the training speed while obtaining the accurate segmentation of multiple regions.

Supplementary Information: The online version contains supplementary material available at 10.1007/s11390-021-0782-5.
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http://dx.doi.org/10.1007/s11390-021-0782-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044657PMC
March 2021

Risk factors to discriminate neoplastic polypoid lesions of gallbladder: A large scale, case-series study.

Asian J Surg 2021 Mar 23. Epub 2021 Mar 23.

Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China. Electronic address:

Background: Polypoid lesion of gallbladder (PLG) size larger than 10 mm is considered to be one of the surgical indications, but the final pathological results are mostly non-neoplastic polyps. The aim of the study was to define the risk factors to discriminate neoplastic PLG and create more precise criteria for surgical indications.

Methods: A large scale, case-series study based on 2704 patients who underwent cholecystectomy for PLG was designed. Logistic regression analysis and receiver operating characteristic curve (ROC) was adopted to identify risk factors and the optimal size criteria for predicting neoplastic PLG.

Results: Patients in the neoplastic group were significantly older than those in the non-neoplastic group and the average PLG size is much larger in the neoplastic group (18.5 ± 4.7 mm vs 12.6 ± 3.6 mm). Neoplastic PLGs are prone to be single and non-neoplastic polyps are usually multiple. On Multivariate logistic regression analysis, PLG size larger than 15 mm and age older than 43 years were found to be the independent risk factors to discriminate neoplastic PLG (Odds ratio 3.546 and 2.77 respectively). The ROC curve showed that 12 mm might be the more reasonable PLG size threshold for the surgical suggestion.

Conclusions: Considering its moderate diagnostic accuracy, the size of gallbladder polyp larger than 10 mm is insufficient to indicate surgical therapy for PLG and 12 mm should be the more optimal polyp's size threshold. Patients older than 43 years have a higher risk of having neoplastic polyps.
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http://dx.doi.org/10.1016/j.asjsur.2021.03.003DOI Listing
March 2021

ML-Net: Multi-Channel Lightweight Network for Detecting Myocardial Infarction.

IEEE J Biomed Health Inform 2021 Feb 19;PP. Epub 2021 Feb 19.

Due to the complexity of myocardial infarction (MI) waveform, most traditional automatic diagnosis models rarely detect it, while those able to detect MI often require high computing and storage capacity, rendering them unsuitable for portable devices. Therefore, in order for convenient real-time MI detection, it is essential to design lightweight models suitable for resource-limited portable devices. This paper proposes a novel multi-channel lightweight model (ML-Net), that provides a new solution for portable detection devices with limited resources. In ML-Net, each electrocardiogram (ECG) lead is assigned an independent channel, ensuring data independence and preserve the ECG characteristics of different angles represented by different leads. Moreover, convolution kernels of heterogeneous sizes are utilized to achieve accurate classification with only a small amount of lead data. Extensive experiments over actual ECG data from the PTB diagnostic database are conducted to evaluate ML-Net. The results show that ML-Net outperforms comparable schemes in diagnosing MI, and it requires lower computational cost and less memory, so that portable devices can be more widely used in the field of Internet of Medical Things(IoMT).
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http://dx.doi.org/10.1109/JBHI.2021.3060433DOI Listing
February 2021

A novel predict factor that increases the success rate of methotrexate treatment in fallopian tube pregnancy.

Ann Transl Med 2021 Jan;9(2):146

International Peace Maternity and Child Health Hospital of China Welfare Institution (IPMCH), School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Embryo Original Diseases, Shanghai Municipal Key Clinical Specialty, Shanghai, China.

Background: For stable fallopian tube pregnancy (FTP), methotrexate (MTX) therapy is reported to be as effective as laparoscopy. However, some cases would need further treatment, e.g., another dose of MTX or laparoscopy. This study is to investigate the potential factors during the treatment of FTP that may facilitate the prediction of a successful outcome of MTX therapy.

Methods: All FTP cases admitted to the International Peace Maternal and Child Health Hospital (IPMCH), Shanghai, China from January 2016 to December 2017 were reviewed. All patients received a single dose of 50 mg/m MTX prior to other treatment. Statistical analysis was performed to determine the correlation between clinical parameters and the success rate of MTX treatment.

Results: The success rate of single-dose MTX was 77.53%. The serum beta-human chorionic gonadotropin (β-hCG) level cut-off value was 452.64 IU/L, with a specificity of 76.7% and sensitivity of 43% [area under the receiver operating characteristic curve (AUC) 0.803; P<0.0001]. In addition, serum β-hCG levels and patient age correlated with the success rate of MTX treatment.

Conclusions: Lower β-hCG levels led to successful MTX treatment for FTP, with a cutoff value of 452.64 IU/L. Younger patients were more sensitive to MTX treatment. These results may help clinicians when deciding the potential therapy for patients with tubal ectopic pregnancies.
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http://dx.doi.org/10.21037/atm-20-7914DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867891PMC
January 2021

Mining Daily Activity Chains from Large-Scale Mobile Phone Location Data.

Cities 2021 Feb;109:103013

Academy of Digital China (Fujian), Fuzhou University, Fuzhou, China.

Understanding residents' daily activity chains provides critical support for various applications in transportation, public health and many other related fields. Recently, mobile phone location datasets have been suggested for mining activity patterns because of their utility and large sample sizes. Although recently machine learning-based models seem to perform well in activity purpose inference using mobile phone location data, most of these models work as black boxes. To address these challenges, this study proposes a flexible white box method to mine human activity chains from large-scale mobile phone location data by integrating both the spatial and temporal features of daily activities with varying weights. We find that the frequency distribution of major activity chain patterns agrees well with the patterns derived based on a travel survey of Shenzhen and a state-of-the-art method. Moreover, a dataset covering over 16.5% of the city population can yield a reasonable outcome of the major activity patterns. The contributions of this study not only lie in offering an effective approach to mining daily activity chains from mobile phone location data but also involve investigating the impact of different data conditions on the model performance, which make using big trajectory data more practical for domain experts.
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http://dx.doi.org/10.1016/j.cities.2020.103013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7809620PMC
February 2021

Value of KPNA4 as a diagnostic and prognostic biomarker for hepatocellular carcinoma.

Aging (Albany NY) 2021 02 1;13(4):5263-5283. Epub 2021 Feb 1.

Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong, China.

It is important to identify novel biomarkers to improve hepatocellular carcinoma (HCC) diagnosis and treatment. Herein, we reported the role of karyopherin α4 (KPNA4) in HCC patients through public data mining and examined the results using clinical samples in our center. Our results revealed that KPNA4 expression level was positively correlated with the infiltration of CD8 T cells, B cells, dendritic cells, CD4 T cells, neutrophils and macrophages. In addition, KPNA4 expression was significantly associated with T cell exhaustion. KPNA4 mRNA and protein expression levels were significantly higher in cancerous tissue than in normal tissue. Besides, the increased expression of KPNA4 indicated poor overall survival. Univariate and multivariate Cox regression analyses showed KPNA4 could be viewed as an independent risk factor for HCC patients. Moreover, our experimental results were consistent with those obtained from bioinformatic results. These findings revealed KPNA4 may serve as a novel prognostic biomarker and a potential therapeutic target for HCC.
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http://dx.doi.org/10.18632/aging.202447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950262PMC
February 2021
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