Publications by authors named "Nan Li"

2,966 Publications

  • Page 1 of 1

A Novel Approach Combined with MIPO Technique for the Treatment of Type C Pilon Fractures.

Oxid Med Cell Longev 2022 14;2022:7427255. Epub 2022 Jun 14.

Department of Orthopedics, Chongqing University Three Gorges Hospital, Chongqing 404000, China.

Objective: Type C fracture is a complete intra-articular fracture, and the mainstay of treatment remains open reduction and internal fixation. The purpose of the study is to observe the clinical effect of an anterior ankle C approach (ankle-C) combined with minimal invasive plate osteosystems (MIPO) for tibial pilon fractures (AO/OTA 43C, combined with fibula fractures).

Methods: A retrospective comparative analysis was performed on the clinical data of 33 patients with C-type pilon fractures (combined fibula fractures) admitted to our department from July 2018 to July 2021, including 12 cases treated with ankle-C (a-C) approach and 21 cases with conventional approach (including combined approach). All patients were followed up for over 6 months. Visual Analogue Scale (VAS), AOFAS Ankle-Hindfoot Scale (AOFAS-AHS), wound healing time, fracture healing time, and complications were used to evaluate the clinical efficacy.

Results: The scores of VAS and AOFAS in the a-C group scored better than the conventional group ( < 0.05), especially in the extent of limited range of motion (LROM) of ankle dorsiflexion-plantarflexion in 1 month after operation and at the last follow-up ( < 0.01). Bone healing was achieved in both groups 6 months after operation, with no implant exposure or infection. Among them, 4 cases in the conventional approach group had wound healing time exceeding 2 weeks.

Conclusions: For type C pilon fractures (combined with fibula fractures), ankle-C approach combined with MIPO technique has certain advantages in ankle function recovery and soft tissue repair, which provides an alternative for the treatment of type C pilon fractures.
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http://dx.doi.org/10.1155/2022/7427255DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213148PMC
June 2022

A Novel Microshear Geometry for Exploring the Influence of Void Swelling on the Mechanical Properties Induced by MeV Heavy Ion Irradiation.

Materials (Basel) 2022 Jun 15;15(12). Epub 2022 Jun 15.

Center for Integrated Nanotechnologies, Los Alamos National Lab, Los Alamos, NM 87545, USA.

Small disks are often the specimen of choice for exposure in nuclear reactor environments, and this geometry invariably limits the types of mechanical testing that can be performed on the specimen. Recently, shear punch testing has been utilized to evaluate changes arising from neutron irradiation in test reactor environments on these small disk specimens. As part of a broader effort to link accelerated testing using ion irradiation and conventional neutron irradiation techniques, a novel microshear specimen geometry was developed for use with heavy-ion irradiated specimens. The technique was demonstrated in pure Cu irradiated to 11 and 110 peak dpa with 10 MeV Cu ions. At 11 peak dpa, the Cu specimen had a high density of small voids in the irradiated region, while at 110 peak dpa, larger voids with an average void swelling of ~20% were observed. Micropillar and microshear specimens both exhibited hardening at 11 dpa, followed by softening at 110 dpa. The close alignment of the new microshear technique and more conventional micropillar testing, and the fact that both follow intuition, is a good first step towards applying microshear testing to a wider range of irradiated materials.
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http://dx.doi.org/10.3390/ma15124253DOI Listing
June 2022

Federated Learning Empowered Real-Time Medical Data Processing Method for Smart Healthcare.

IEEE/ACM Trans Comput Biol Bioinform 2022 Jun 23;PP. Epub 2022 Jun 23.

Computer-aided diagnosis (CAD) has always been an important research topic for applying artificial intelligence in smart healthcare. Sufficient medical data are one of the most critical factors in CAD research. However, medical data are usually obtained in chronological order and cannot be collected all at once, which poses difficulties for the application of deep learning technology in the medical field. The traditional batch learning method consumes considerable time and space resources for real-time medical data, and the incremental learning method often leads to catastrophic forgetting. To solve these problems, we propose a real-time medical data processing method based on federated learning. We divide the process into the model stage and the exemplar stage. In the model stage, we use the federated learning method to fuse the old and new models to mitigate the catastrophic forgetting problem of the new model. In the exemplar stage, we use the most representative exemplars selected from the old data to help the new model review the old knowledge, which further mitigates the catastrophic forgetting problem of the new model. We use this method to conduct experiments on a simulated medical real-time data stream. The experimental results show that our method can learn a disease diagnosis model from a continuous medical real-time data stream. As the amount of data increases, the performance of the disease diagnosis model continues to improve, and the catastrophic forgetting problem has been effectively mitigated. Compared with the traditional batch learning method, our method can significantly save time and space resources.
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http://dx.doi.org/10.1109/TCBB.2022.3185395DOI Listing
June 2022

Expanded Application of a Photoaffinity Probe to Study Epidermal Growth Factor Receptor Tyrosine Kinase with Functional Activity.

Anal Chem 2022 Jun 21. Epub 2022 Jun 21.

Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.

The abnormal activation of the epidermal growth factor receptor (EGFR) is strongly associated with cancer invasion and metastasis. Tools and methods are required to study and visualize EGFR activation under (patho)physiological conditions. Here, we report the development of a two-step photoaffinity probe (HX101) by incorporation of a diazirine as a photoreactive group and an alkyne as a ligation handle to quantitively study EGFR kinase activity in native cellular contexts and human tissue slices. HX101 is a multifunctional probe based on the pharmacophore of the EGFR tyrosine kinase inhibitor (EGFR-TKI) and can covalently target the EGFR upon photoactivation. The incorporated alkyne serves as a versatile ligation handle and enables HX101 to introduce distinct reporter groups (e.g., fluorophore and biotin) via click chemistry. With variable reporter tags, HX101 enables visualization and target engagement studies of the active EGFR in a panel of cancer cells using flow cytometry, confocal microscopy, and mass spectrometry. Furthermore, as a proof of concept study, we applied HX101 in stochastic optical reconstruction microscopy super-resolution imaging to study EGFR activation in live cells. Importantly, HX101 was also applied to visualize EGFR mutant activity in tumor tissues from lung cancer patients for prediction of EGFR-TKI sensitivity. Altogether, our results demonstrate the wide application of a selective photoaffinity probe in multi-modal assessment/visualization of EGFR activity in both live cells and tissue slices. We anticipate that these diverse applications can facilitate the translation of a strategically functionalized probe into medical use.
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http://dx.doi.org/10.1021/acs.analchem.2c01340DOI Listing
June 2022

Altered polymerase theta expression promotes chromosomal instability in salivary adenoid cystic carcinoma.

J Cell Mol Med 2022 Jun 21. Epub 2022 Jun 21.

College of Stomatology, Dalian Medical University, Dalian, China.

Genomic instability (GIN) plays a key role in cancer progression. The disorders of polymerase theta (POLQ) were reported to contribute to GIN and progression in many cancers. Here, we found that POLQ over-expression was related to salivary adenoid cystic carcinoma (SACC) progression and poor prognosis. Then, we investigated the role and mechanism of POLQ in the GIN in SACC. GIN was assessed by chromosome staining with DAPI and Giemsa, as well as qRT-PCR of the mitosis-related gene expression. Meanwhile, PCR-SSCP was used to evaluate microsatellite instability. Modulation of POLQ expression increased chromosomal instability and enhanced the sensitivity to etoposide without impacting microsatellite stability. Mechanistically, POLQ regulated genome stability by promoting the expression of the error-prone alt-NHEJ-related protein PARP1, and down-regulating c-NHEJ- and HR-related proteins KU70 and RAD51. In vitro CCK, Transwell assays and in vivo murine xenograft models indicated that the PARP inhibitor olaparib suppressed SACC growth in the case of etoposide-induced DNA damage. Bioinformatic analysis identified CEBPB as a potential POLQ-regulating transcription factor. In summary, our research provides new insights into the mechanisms of SACC chromosomal instability and identifies new potential targets for SACC treatment.
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http://dx.doi.org/10.1111/jcmm.17429DOI Listing
June 2022

Impact of pre-oxidation on the formation of byproducts in algae-laden water disinfection: Insights from fluorescent and molecular weight.

J Environ Sci (China) 2022 Jul 4;117:21-27. Epub 2022 Jan 4.

Key Laboratory of Building Safety and Energy Efficiency, Ministry of Education, Department of Water Engineering and Science, College of Civil Engineering, Hunan University, Changsha 410082, China. Electronic address:

Pre-oxidation has been reported to be an effective way to remove algal cells in water, but the released algal organic matter (AOM) could be oxidized and lead to the increment in disinfection by-product (DBP) formation. The relationship between pre-oxidation and AOM-derived DBP formation needs to be approached more precisely. This study compared the impact of four pre-oxidants, ozone (O), chlorine dioxide (ClO), potassium permanganate (KMnO) and sodium hypochlorite (NaClO), on the formation of nitrogenous (N-) and carbonaceous (C-) DBPs in AOM chlorination. The characterization (fluorescent properties, molecular weight distribution and amino acids concentration) on AOM samples showed that the characterization properties variations after pre-oxidation were highly dependent on the oxidizing ability of oxidants. The disinfection experiments showed that O increased DBP formation most significantly, which was consistent with the result of characterization properties variations. Then canonical correspondent analysis (CCA) and Pearson's correlation analysis were conducted based on the characterization data and DBP formation. CCA indicated that C-DBPs formation was highly dependent on fluorescent data. The formation of haloacetic acids (HAAs) had a positive correlation with aromatic protein-like component while trichloromethane (TCM) had a positive correlation with fulvic acid-like component. Pearson's correlation analysis showed that low molecular weight fractions were favorable to form N-DBPs. Therefore, characterization data could provide the advantages in the control of DBP formation, which further revealed that KMnO and ClO were better options for removing algal cells as well as limiting DBP formation.
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http://dx.doi.org/10.1016/j.jes.2021.12.021DOI Listing
July 2022

Artesunate Therapy Alleviates Fracture-Associated Chronic Pain After Orthopedic Surgery by Suppressing CCL21-Dependent TREM2/DAP12 Inflammatory Signaling in Mice.

Front Pharmacol 2022 2;13:894963. Epub 2022 Jun 2.

Department of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, China.

Chronic pain after bone fracture and orthopedic surgery is often refractory to most analgesics currently in use, thus emphasizing the urgent need for improved therapeutic medications. Chemokine-dependent neuroinflammation is critical for excitatory synaptic plasticity and central nociception sensitization. Recent studies have focused on the inhibition of inflammatory responses by artesunate, the first anti-malaria drug extracted from artemisinin. The present study investigated the analgesic effects and potential targets of artesunate in a mouse model of chronic pain induced by tibial fracture and orthopedic surgery. Three injections of artesunate were intrathecally administered on a daily basis from days 4 to 6 after fracture. We reported that repetitive exposure to artesunate (10 and 100 μg but not 1 μg) dose-dependently prevented fracture-induced mechanical and cold allodynia. Moreover, single intrathecal injection of artesunate (100 μg) alleviated the established chronic pain on day 14 after fracture surgery. Intraperitoneal artesunate (10 and 50 mg kg) therapy was effective against chronic fracture pain. Intriguingly, artesunate inhibited the upregulation of spinal chemokine CCL21, triggering receptor expressed on myeloid cells 2 (TREM2) and DNAX-activating protein of 12 kDa (DAP12) expressions and microglia activation in fracture mice. Furthermore, spinal CCL21 neutralization attenuated the severity of fracture-associated post-surgical pain. Exogenous CCL21-induced acute inflammatory pain was impaired by artesunate therapy. Additionally, the pharmacological blockage of TREM2 reduced recombinant CCL21-elicited behavioral hypernociception. The present findings demonstrate that artesunate therapy reduces the initiation and maintenance of fracture-associated chronic postoperative pain by inhibiting CCL21-dependent TREM2/DAP12 inflammatory signaling and microglia activation, thus suggesting that artesunate could emerge as a therapeutic strategy for fracture pain management.
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http://dx.doi.org/10.3389/fphar.2022.894963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202025PMC
June 2022

Inhibitory mechanism of tangeretin, a citrus flavone on the sphingosylphosphorylcholine (SPC)-induced vascular smooth muscle contraction.

J Pharmacol Sci 2022 Aug 13;149(4):189-197. Epub 2022 May 13.

Department of Advanced Preventive Medicine, School of Medicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, 755-8505, Japan. Electronic address:

We previously discovered that the SPC/Fyn/Rho-kinase (ROK) pathway mediates the Ca-sensitization of coronary arterial smooth muscle (CASM) contraction leading to vasospasm, a major cause of sudden death. Lately, we have been trying to find and develop more natural edible compounds which can treat and/or prevent the SPC-induced abnormal CASM contraction, and finally the first to discover that tangeretin (5,6,7,8,4'-pentamethoxyflavone), a natural compound extracted from citrus plants, can inhibit the SPC-induced CASM contraction both in the pretreatment and posttreatment. In porcine CASM tissues, tangeretin showed remarkable inhibitory effects on the SPC-induced contraction with modest inhibitory effects on the high K-depolarization-induced Ca-dependent contraction, both in pretreatment and posttreatment at the optimal concentrations; Regarding the mechanisms, tangeretin markedly abolished the SPC-induced cell contraction through inhibiting the SPC-induced activation and translocation of Fyn and ROK from the cytoplasm to the cell membrane in cultured CASM cells, resulting in the reduction of phosphorylation of myosin light chain. Taken together, these findings indicate that tangeretin, upon pre- or post-treatment, inhibits the SPC-induced CASM contraction through suppressing the Fyn/ROK signaling pathway, thereby suggesting that tangeretin can be a potential candidate for the treatment and/or prevention of vasospasm.
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http://dx.doi.org/10.1016/j.jphs.2022.05.002DOI Listing
August 2022

Antibiotic pollution in lakes in China: Emission estimation and fate modeling using a temperature-dependent multimedia model.

Sci Total Environ 2022 Jun 15:156633. Epub 2022 Jun 15.

SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China.

The high use of antibiotics worldwide has poses a serious threat to both human and environmental health. Lakes are served as reservoirs for antibiotics, however, there is a lack of information available on antibiotics emissions and the subsequent pollution. Here, the emission and fate of 34 frequently detected antibiotics were studied in 226 Chinese lakes, via the built emission estimation method and a temperature-dependent multimedia lake model. It has been estimated that totally 5711 tons antibiotic were discharged into the lakes of China in 2019. Antibiotics emissions are due to human activities, with 3800-fold higher emissions in the Eastern China than that in Western China. The antibiotic fate in lakes has been successfully modelled by simulating the lake stratification, freeze-melt cycles and the stable condition throughout the year. Both stratification and freeze-melt cycles can lead to increased antibiotic concentrations in lake water. Deep-water lakes were shown to serve as a highly effective natural storage medium for antibiotics. The reliability of the model was confirmed by published measured concentrations and Monte Carlo method. This is the first study to comprehensively investigate the antibiotic fate in lakes of China, providing valuable guidance for the remediation of contaminated lakes.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156633DOI Listing
June 2022

[Anti-inflammation effect of moxibustion for rats with diarrhea-predominant irritable bowel syndrome based on multiple miRNAs regulating NF-κB signal pathway].

Zhongguo Zhen Jiu 2022 Jun;42(6):654-62

Second Affiliated Hospital of Anhui University of CM, Hefei 230061; Anhui Acupuncture Clinical Medical Research Center, Hefei 230061; Clinical Institute of Acupuncture and Moxibustion, Anhui Academy of TCM, Hefei 230038.

Objective: To observe the effect of moxibustion on the regulation of nuclear factor-kappa B (NF-κB) and inflammatory factors by multiple microRNAs (miRNAs) in rats with diarrhea-predominant irritable bowel syndrome (IBS-D), and to explore the anti-inflammatory mechanism of moxibustion on IBS-D.

Methods: Twelve of 52 newborn rats were randomly selected into a normal group. The remaining rats were made into IBS-D model. A total of 36 rats with successful model were randomly divided into a model group, a medication group and a moxibustion group, 12 rats in each group. The rats in the medication group were intraperitoneally injected with pyrrolidine dithiocarbamate (PDTC). The rats in the moxibustion group were treated with moxibustion at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) for 20 min each time. All the intervention was given once a day for 7 days. Before and after modeling as well as after intervention, the body mass, loose stool rate and the minimum volume threshold of abdominal withdrawal reflex (AWR) were measured. After intervention, the contents of serum tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-8 were detected by ELISA method; the morphology of colon tissues was observed by HE staining, and the expressions of miR-155, miR-125b, miR-29b, miR-31, miR-18a and NF-κB p65 mRNA in colon tissues were detected by real-time PCR. The expressions of NF-κB p65, TNF-α, IL-1β and IL-8 protein in colon tissues were detected by immunofluorescence.

Results: After modeling, the body mass and the minimum volume threshold of AWR in the model group were lower than those in the normal group (<0.01); the rates of loose stool in the model group were higher than those in the normal group (<0.01); after intervention, in the model group, the inflammatory infiltration of colon tissues was obvious, and the serum levels of TNF-α, IL-1 β, IL-8 were higher than those in the normal group (<0.05); the expression of miR-155, miR-125b, miR-29b, miR-31, miR-18a and NF-κB p65 mRNA in colon tissues was higher than that in the normal group (<0.05); the protein expression of NF-κB p65, TNF-α, IL-1β, IL-8 was also higher than that in the normal group (<0.01). After intervention, the body mass and the minimum volume threshold of AWR in the medication group and the moxibustion group were both higher than those in the model group (<0.05); the loose stool rate in the medication group and the moxibustion group were lower than those in model group (<0.05); the inflammatory cells infiltration in the colon tissues was less, the serum levels of TNF-α, IL-1β and IL-8 as well as the protein expression of NF-κB p65, TNF-α, IL-1β and IL-8 in the colon tissues in the medication group and the moxibustion group were lower than those in the model group (<0.05, <0.01). The expression of miR-125b, miR-31, miR-18a and NF-κB p65 mRNA in the medication group were lower than those in the model group (<0.05). The expression of miR-155, miR-125b, miR-29b, miR-31, miR-18a and NF-κB p65 mRNA in the moxibustion group were lower than those in the model group (<0.05). The miR-155, miR-125b, miR-29b, miR-31, miR-18a were positively correlated with NF-κB p65 mRNA (0<<1, <0.01).

Conclusion: The anti-inflammatory mechanism of moxibustion at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) for IBS-D rats may be related to regulating multiple miRNAs to inhibit NF-κB signal pathway and reduce the expression of inflammatory factors.
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http://dx.doi.org/10.13703/j.0255-2930.20210521-k0002DOI Listing
June 2022

Association of Pre-stroke Frailty With Prognosis of Elderly Patients With Acute Cerebral Infarction: A Cohort Study.

Front Neurol 2022 30;13:855532. Epub 2022 May 30.

Department of Neurology, Huadong Hospital Affiliated to Fudan University, Shanghai, China.

Background: Frailty is a state of cumulative degradation of physiological functions that leads to adverse outcomes such as disability or mortality. Currently, there is still little understanding of the prognosis of pre-stroke frailty status with acute cerebral infarction in the elderly.

Objective: We investigated the association between pre-stroke frailty status, 28-day and 1-year survival outcomes, and functional recovery after acute cerebral infarction.

Methods: Clinical data were collected from 314 patients with acute cerebral infarction aged 65-99 years. A total of 261 patients completed follow-up in the survival cohort analysis and 215 patients in the functional recovery cohort analysis. Pre-stroke frailty status was assessed using the FRAIL score, the prognosis was assessed using the modified Rankin Scale (mRS), and disease severity using the National Institutes of Health Stroke Scale (NIHSS).

Results: Frailty was independently associated with 28-day mortality in the survival analysis cohort [hazard ratio (HR) = 4.30, 95% 1.35-13.67, = 0.014]. However, frailty had no independent effect on 1-year mortality ( = 1.47, 95% 0.78-2.79, = 0.237), but it was independently associated with advanced age, the severity of cerebral infarction, and combined infection during hospitalization. Logistic regression analysis after adjusting for potential confounders in the functional recovery cohort revealed frailty, and the NIHSS score was significantly associated with post-stroke severe disability (mRS > 2) at 28 days [pre-frailty adjusted odds ratio (a): 8.86, 95% 3.07-25.58, < 0.001; frailty a: 7.68, 95% 2.03-29.12, = 0.002] or 1 year (pre-frailty a: 8.86, 95% 3.07-25.58, < 0.001; frailty a: 7.68, 95% 2.03-29.12, = 0.003).

Conclusions: Pre-stroke frailty is an independent risk factor for 28-day mortality and 28-day or 1-year severe disability. Age, the NIHSS score, and co-infection are likewise independent risk factors for 1-year mortality.
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http://dx.doi.org/10.3389/fneur.2022.855532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196308PMC
May 2022

Effect of Routine Gastric Residual Aspiration on the Preterm Infant Fecal Microbiome.

Am J Perinatol 2022 Jun 16. Epub 2022 Jun 16.

University of Florida, Gainesville, United States.

Objective: Enteral feeding tubes are used in neonatal intensive care units (NICU) to assess feeding tolerance by utilizing pre-prandial gastric residual aspiration. This study evaluates the effect of gastric residual aspiration on the preterm infant fecal microbiome and gastrointestinal inflammation.

Study Design: Fifty-one very low birth weight (VLBW) infants (<32 weeks gestational age and <1250g) enrolled in a larger single-center randomized controlled trial evaluating the effects of routine and non-routine gastric residual aspiration were selected for further analysis. Of those infants, 30 infants had microbiome analysis performed on stools collected at 6 weeks by sequencing the bacterial V1-V3 variable regions of the genes encoding for 16S rRNA. In an additional 21 infants, stool samples collected at 3- and 6-weeks were analyzed for intestinal inflammation using a cytokine multiplex panel.

Results: Microbial communities between groups were not distinct from each other and there was no difference in intestinal inflammation between groups. Analyses using gene expression packages DESeq2 and edgeR produced statistically significant differences in several taxa possibly indicating a more commensal intestinal microbiome in infants not undergoing gastric residual aspiration.

Conclusion: Omission of routine gastric residual aspiration was not associated with intestinal dysbiosis or inflammation, providing additional evidence that monitoring pre-prandial gastric residuals is unnecessary.
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http://dx.doi.org/10.1055/a-1877-6306DOI Listing
June 2022

Prevalence and related factors of hyperuricaemia in Chinese children and adolescents: a pooled analysis of 11 population-based studies.

Ann Med 2022 Dec;54(1):1608-1615

Center for Non-communicable Disease Management, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, PR China.

Background And Aims: Hyperuricaemia can lead to gout and is associated with an increased risk of cardiometabolic disease. We aimed to investigate the prevalence of hyperuricaemia and its related factors in Chinese children and adolescents.

Methods: We pooled data from 11 population-based studies comprising 54,580 participants aged 3-19 years. The sex- and age-standardized prevalence of hyperuricaemia was estimated overall and by sex, age, weight status, geographic region and survey year.

Results: Serum uric acid (SUA) increased gradually from 3 to 11 years with no significant sex difference, and then increased dramatically during 11-15 years. The estimated overall prevalence of hyperuricaemia was 23.3% (26.6% in boys and 19.8% in girls,  < .001). The prevalence increased with growing age (3.7, 9.8, 15.8, 35.5 and 31.7% among children aged 3-5, 6-8, 9-11, 12-15 and 16-19 years, respectively, for trend < .001) and with increasing weight status (18.2, 37.6, 50.6 and 64.5% among children with non-overweight, overweight, obesity and extreme obesity, respectively, for trend < .001). The prevalence was higher in North than in South (24.2 . 19.7%,  < .001), and increased markedly from 16.7% during 2009-2015 to 24.8% during 2016-2019. In multivariable regression analyses, sex, age, obesity, region and survey year were independently associated with odds of hyperuricaemia.

Conclusions: The prevalence of hyperuricaemia in Chinese children and adolescents is unexpectedly high. The findings suggest an urgent need to implement effective interventions to reduce risk of hyperuricaemia in Chinese youths.KEY MESSAGES What is the prevalence of hyperuricaemia in Chinese children and adolescents? In this large pooled cross-sectional study comprising >50,000 children and adolescents aged 3-19 years, we found that the prevalence of hyperuricaemia was high in overall population and subgroups of sex, age, obesity, region and survey year. Our findings indicate that hyperuricaemia is an important health problem in Chinese children and adolescents, and effective intervention strategies are needed to reduce its burden.
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http://dx.doi.org/10.1080/07853890.2022.2083670DOI Listing
December 2022

Artesunate Alleviates Paclitaxel-Induced Neuropathic Pain in Mice by Decreasing Metabotropic Glutamate Receptor 5 Activity and Neuroinflammation in Primary Sensory Neurons.

Front Mol Neurosci 2022 27;15:902572. Epub 2022 May 27.

Department of Anesthesiology, Tianjin Research Institute of Anesthesiology, Tianjin Medical University General Hospital, Tianjin, China.

Experimental studies on the pathogenetic process of paclitaxel-induced neuropathic pain (PINP) have been initially carried out, but PINP still has no effective therapy. Recently reported studies have highlighted the involvement of glutamate receptors and neuroinflammation in peripheral and central nociceptive transmission in PINP. Artesunate is a first-line antimalarial drug with established efficacy in alleviating pain in a variety of pathologies. The current work assessed whether artesunate inhibits PINP by modulating metabotropic glutamate receptor 5 (mGluR5) and neuroinflammation in mice. The anti-hyperalgesic effect of artesunate was verified by assessing mechanical frequency and thermal latency in the paw withdrawal test as well as spontaneous pain. The expression levels of mGluR5, pain-related receptors and neuroinflammatory markers in dorsal root ganglion (DRG) were examined. In addition, treatment with CHPG and 2-methyl-6-(phenyl ethynyl) pyridine (MPEP) (mGluR5 agonist and antagonist, respectively) was performed to determine mGluR5's role in the anti-hyperalgesic properties of artesunate. We demonstrated artesunate prevented PINP in a dose-dependent manner, while exerting a clear anti-hyperalgesic effect on already existing PINP. Artesunate normalized paclitaxel-related expression changes in DRG mGluR5, NR1, and GluA2, as well as six paclitaxel related neuroinflammation markers. Intrathecal application of MPEP treated PINP by reversing NR1 and GluA2 expression changes but had no effects on chemokines and inflammatory factors. Furthermore, artesunate treatment reversed acute pain following CHPG application. In conclusion, this study revealed that artesunate alleviates paclitaxel-induced hyperalgesia and spontaneous pain by decreasing DRG mGluR5 expression and neuroinflammation in the mouse model of PINP.
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http://dx.doi.org/10.3389/fnmol.2022.902572DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184756PMC
May 2022

The Chemokines Initiating and Maintaining Immune Hot Phenotype Are Prognostic in ICB of HNSCC.

Front Genet 2022 27;13:820065. Epub 2022 May 27.

Department of Oral Pathology, School of Stomatology, Dalian Medical University, Dalian, China.

The immune checkpoint blockade (ICB) with anti-programmed cell death protein 1(PD-1) on HNSCC is not as effective as on other tumors. In this study, we try to find out the key factors in the heterogeneous tumor-associated monocyte/macrophage (TAMM) that could regulate immune responses and predict the validity of ICB on HNSCC. To explore the correlation of the TAMM heterogeneity with the immune properties and prognosis of HNSCC, we established the differentiation trajectory of TAMM by analyzing the single-cell RNA-seq data of HNSCC, by which the HNSCC patients were divided into different sub-populations. Then, we exploited the topology of the network to screen out the genes critical for immune hot phenotype of HNSCC, as well as their roles in TAMM differentiation, tumor immune cycle, and progression. Finally, these key genes were used to construct a neural net model via deep-learning framework to predict the validity of treatment with anti-PD-1/PDL-1 According to the differentiation trajectory, the genes involved in TAMM differentiation were categorized into early and later groups. Then, the early group genes divided the HNSCC patients into sub-populations with more detailed immune properties. Through network topology, CXCL9, 10, 11, and CLL5 related to TAMM differentiation in the TME were identified as the key genes initiating and maintaining the immune hot phenotype in HNSCC by remarkably strengthening immune responses and infiltration. Genome wide, CASP8 mutations were found to be key to triggering immune responses in the immune hot phenotype. On the other hand, in the immune cold phenotype, the evident changes in CNV resulted in immune evasion by disrupting immune balance. Finally, based on the framework of CXCL9-11, CLL5, CD8, CD4 T cells, and Macrophage M1, the neural network model could predict the validity of PD-1/PDL-1 therapy with 75% of AUC in the test cohort. We concluded that the CXCL9, 10,11, and CCL5 mediated TAMM differentiation and constructed immune hot phenotype of HNSCC. Since they positively regulated immune cells and immune cycle in HNSCC, the CXCL9-11 and CCL5 could be used to predict the effects of anti-PD-1/PDL-1 therapy on HNSCC.
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http://dx.doi.org/10.3389/fgene.2022.820065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186378PMC
May 2022

Both prokaryotes and eukaryotes produce an immune response against plasmids with 5'-GTTTGTT-3'.

Cell Biosci 2022 Jun 11;12(1):87. Epub 2022 Jun 11.

Model Animal Research Center, Medical School, Nanjing University, NanjingJiangsu, 210061, China.

In the evolutionary "arms race" from prokaryotes to eukaryotes, some memories of foreign DNA have been conserved for defensive purposes. Shortly after invasion by the plasmid, pEGFP-N1, the conserved the defense gene, isg15, was activated in the zebrafish zygote and in mammalian cells. Based on the sequence similarity, we found three virus-derived sequences in pEGFP-N1 which share the 5'-GTTTGTT-3' core sequence, an epigenetic factor leading to increased expression of isg15. Mutation of the core sequence greatly reduces the degradation rate of the plasmid in E. coli cells or zebrafish embryos. We conclude that a conserved defense response, common to both eukaryotic and prokaryotic cells, allows identification and degradation of plasmids containing 5'-GTTTGTT-3'.
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http://dx.doi.org/10.1186/s13578-022-00825-3DOI Listing
June 2022

Association between trimethylamine N-oxide and prognosis of patients with acute myocardial infarction and heart failure.

ESC Heart Fail 2022 Jun 9. Epub 2022 Jun 9.

Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

Aims: This study aimed to investigate the association between trimethylamine N-oxide (TMAO) and the prognosis and association between high-sensitivity C-reactive protein (hsCRP) and TMAO-associated cardiovascular risk in patients with acute myocardial infarction (AMI) complicated by heart failure (HF).

Methods And Results: A total of 985 patients presenting with AMI and HF were consecutively enrolled at the Fuwai Hospital between March 2017 and January 2020. Patients were stratified into groups according to tertiles of TMAO levels and the median hsCRP levels. The primary endpoint was major adverse cardiac events (MACE), including all-cause death, recurrence of myocardial infarction, and rehospitalization due to HF. During a median follow-up of 716 days, 138 (14.0%) patients experienced MACE. Cox regression analyses showed that the adjusted hazard ratio (HR) for MACE was higher in patients in tertile 3 [TMAO > 9.52 μmol/L, HR: 1.85, 95% confidence interval (CI): 1.18-2.89; P = 0.007] than in tertile 1 (TMAO < 4.74 μmol/L), whereas no significant differences were detected between the patients in tertiles 1 and 2 (TMAO = 4.74-9.52 μmol/L, HR: 0.96, 95% CI: 0.59-1.58; P = 0.874). Restricted cubic spline regression depicted an S-shaped association between TMAO and MACE (P for nonlinearity = 0.012). In the setting of hsCRP above the median level (6.68 mg/L), per unit increase of TMAO was associated with a 20% increase of MACE risk (HR: 1.20, 95% CI: 1.05-1.37, P = 0.009); increasing tertiles of TMAO were significantly associated with a higher risk of MACE (adjusted P = 0.007 for interaction; P < 0.001 for trend across tertiles). The Kaplan-Meier analysis indicated that patients in tertile 3 had a significantly lower event-free survival (P = 0.001) when the hsCRP level was above the median level. No similar association between TMAO and MACE was observed when the hsCRP level was below the median level.

Conclusions: High plasma TMAO levels were independently correlated with poor prognosis in patients with AMI complicated by HF, especially in those with higher hsCRP levels. There was an S-shaped relationship between TMAO and HR for MACE.
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http://dx.doi.org/10.1002/ehf2.14009DOI Listing
June 2022

Synthesis of a Superhydrophobic Fluorinated Nano-Emulsion and Its Modification on the Wettability of Tight Sandstone.

Materials (Basel) 2022 Jun 6;15(11). Epub 2022 Jun 6.

Daqing Oilfield Production Engineering Research Institute, Daqing 163453, China.

The water-blocking effect is a serious problem when developing tight sandstone gas reservoirs, which can cause a sharp reduction in gas production. Wettability alteration of near-wellbore sand rock surface from superhydrophilicity to superhydrophobicity is an effective method to decrease capillary pressure. In this study, a superhydrophobic fluorinated nano-emulsion was synthesized via a soap-free emulsion polymerization process using methacryloxyethyl trimethyl ammonium chloride, trifluoctyl methacrylate, and styrene as monomers. The effect of the fluorinated monomer concentration on wettability alteration was evaluated by measuring the contact angle of the formation water droplet on the modified glass slides using nano-emulsions with different fluorinated monomer concentrations. The results showed that the nano-emulsion had a good dispersibility and homogeneous particle size of around 90 nm, and with the increase in fluorinated monomer concentration, the contact angle increased. The contact angle was the largest when the fluorinated monomer mass rate concentration reached 50%. The adsorption of nanoparticles could alter the rock wettability from a super hydrophilic state (θ = 7°) to a superhydrophobic state (θ = 150°). The spontaneous imbibition experiments showed that the formation water adsorption quality of the core decreased by 49.7% after being modified by the nano-emulsion. The nano-emulsion showed a good superhydrophobicity and had the potential to be used to reduce the water-blocking damage in the tight gas reservoirs.
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http://dx.doi.org/10.3390/ma15114015DOI Listing
June 2022

Amphiphilic shell nanomagnetic adsorbents for selective and highly efficient capture of low-density lipoprotein from hyperlipidaemia serum.

J Mater Chem B 2022 Jun 9. Epub 2022 Jun 9.

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China.

Removal of low-density lipoprotein (LDL) from hyperlipemia patients' blood represents an effective approach to prevent the progression of atherosclerotic cardiovascular disease. Based on the LDL structural characteristics and intermolecular interactions, a tailored nano-adsorbent ([email protected]@PAA-PE) was prepared aimed at the removal of LDL from hyperlipemia serum with high selectivity. The core-shell structured magnetic nanoparticles were embedded in an amphiphilic layer composed of hydrophilic poly(acrylic acid) and lipophilic phospholipids to provide multifunctional binding for LDL particles. The results of dynamic light scattering, water contact angle and zeta-potential measurements, thermal gravimetric analysis, and X-ray photoelectron spectroscopy together with Fourier transform infrared spectroscopy confirmed the core-shell structured nanoparticles bearing amphiphilic poly acrylic acid and phospholipid molecules. Because of the superior electronegativity of the functional layer, the nano-adsorbent demonstrated favorable adsorption selectivity against high-density lipoprotein, which possesses a similar structure to LDL but has a cardio-protective function in the human body. The respective adsorption capacity of [email protected]@PAA-PE towards LDL, total cholesterol and triglycerides reached up to 6.26 mg g, 8.41 mg g and 9.19 mg g, which was 7.03, 9.45 and 10.32 times that towards HDL (0.89 mg g). The kinetic and isothermal studies revealed that multiple interactions containing both physical and chemical adsorption occurred in the binding procedure between LDL and [email protected]@PAA-PE, and chemical adsorption may play a more predominant role in LDL adsorption. The nano-adsorbent also had negligible effects on blood cells, and possessed satisfactory recyclability, low cytotoxicity and hemolysis ratios, indicating its good application prospects as a hemoperfusion adsorbent in the treatment of hyperlipidaemia.
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http://dx.doi.org/10.1039/d2tb00291dDOI Listing
June 2022

The Efficacy of Orthodontics plus Implant Anchorage in Orthodontic Treatment: A Randomized Controlled Study.

Evid Based Complement Alternat Med 2022 30;2022:4049076. Epub 2022 May 30.

Neurology Department of Jin Zhou Central Hospital, Jin Zhou 121000, China.

Objective: To investigate the efficacy of orthodontics plus implant anchorage in orthodontic treatment.

Methods: This randomized controlled study was conducted on 90 patients who had orthodontic treatment in our hospital between October 2019 and October 2020, and they were assigned to either a control group ( = 45) or an observation group ( = 45) via the random number table method. Patients in the control group received orthodontics while those in the observation group underwent orthodontics plus implant anchorage. The two groups were compared in terms of clinical indexes, efficacy, orthodontic state, adverse reaction rate, quality of life, and satisfaction.

Results: After treatment, orthodontics plus implant anchorage led to lower gingival attachment level, gingival bleeding index, plaque index, and periodontal probing depth versus orthodontics alone ( < 0.05); orthodontics plus implant anchorage contributed to a higher efficacy versus orthodontics alone (91.11% vs. 73.33%, < 0.05); orthodontics plus implant anchorage resulted in smaller molar displacement, larger protrusion distance of the upper central incisor, and larger inclination angle of the upper central incisor ( < 0.05); orthodontics plus implant anchorage was associated with fewer adverse reactions (4.44% vs. 26.67%, < 0.05) and a higher quality of life scores versus orthodontics alone ( < 0.05); orthodontics plus implant anchorage yielded a higher satisfaction level versus orthodontics alone (95.56% vs. 66.67%, < 0.05).

Conclusion: The orthodontics plus implant anchorage offers a promising solution in orthodontic treatment. It is conducive to restoring dental indicators and improving quality of life and satisfaction. It is therefore worthy of application.
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http://dx.doi.org/10.1155/2022/4049076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9170419PMC
May 2022

Crizotinib Shows Antibacterial Activity against Gram-Positive Bacteria by Reducing ATP Production and Targeting the CTP Synthase PyrG.

Microbiol Spectr 2022 Jun 8:e0088422. Epub 2022 Jun 8.

MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan Universitygrid.258164.c, Guangzhou, China.

Infections caused by drug-resistant bacteria are a serious threat to public health worldwide, and the discovery of novel antibacterial compounds is urgently needed. Here, we screened an FDA-approved small-molecule library and found that crizotinib possesses good antimicrobial efficacy against Gram-positive bacteria. Crizotinib was found to increase the survival rate of mice infected with bacteria and decrease pulmonary inflammation activity in an animal model. Furthermore, it showed synergy with clindamycin and gentamicin. Importantly, the Gram-positive bacteria showed a low tendency to develop resistance to crizotinib. Mechanistically, quantitative proteomics and biochemical validation experiments indicated that crizotinib exerted its antibacterial effects by reducing ATP production and pyrimidine metabolism. A drug affinity responsive target stability study suggested crizotinib targets the CTP synthase PyrG, which subsequently disturbs pyrimidine metabolism and eventually reduces DNA synthesis. Subsequent molecular dynamics analysis showed that crizotinib binding occurs in close proximity to the ATP binding pocket of PyrG and causes loss of function of this CTP synthase. Crizotinib is a promising antimicrobial agent and provides a novel choice for the development of treatment for Gram-positive infections. Infections caused by drug-resistant bacteria are a serious problem worldwide. Therefore, there is an urgent need to find novel drugs with good antibacterial activity against multidrug-resistant bacteria. In this study, we found that a repurposed drug, crizotinib, exhibits excellent antibacterial activity against drug-resistant bacteria both and via suppressing ATP production and pyrimidine metabolism. Crizotinib was found to disturb pyrimidine metabolism by targeting the CTP synthase PyrG, thus reducing DNA synthesis. This unique mechanism of action may explain the decreased development of resistance by Staphylococcus aureus to crizotinib. This study provides a potential option for the treatment of drug-resistant bacterial infections in the future.
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http://dx.doi.org/10.1128/spectrum.00884-22DOI Listing
June 2022

An artificial intelligence-based risk prediction model of myocardial infarction.

BMC Bioinformatics 2022 Jun 7;23(1):217. Epub 2022 Jun 7.

Engineering Research Center of Medical Information Technology, Ministry of Education, West China Hospital of Sichuan University, Chengdu, 610041, Sichuan, China.

Background: Myocardial infarction can lead to malignant arrhythmia, heart failure, and sudden death. Clinical studies have shown that early identification of and timely intervention for acute MI can significantly reduce mortality. The traditional MI risk assessment models are subjective, and the data that go into them are difficult to obtain. Generally, the assessment is only conducted among high-risk patient groups.

Objective: To construct an artificial intelligence-based risk prediction model of myocardial infarction (MI) for continuous and active monitoring of inpatients, especially those in noncardiovascular departments, and early warning of MI.

Methods: The imbalanced data contain 59 features, which were constructed into a specific dataset through proportional division, upsampling, downsampling, easy ensemble, and w-easy ensemble. Then, the dataset was traversed using supervised machine learning, with recursive feature elimination as the top-layer algorithm and random forest, gradient boosting decision tree (GBDT), logistic regression, and support vector machine as the bottom-layer algorithms, to select the best model out of many through a variety of evaluation indices.

Results: GBDT was the best bottom-layer algorithm, and downsampling was the best dataset construction method. In the validation set, the F1 score and accuracy of the 24-feature downsampling GBDT model were both 0.84. In the test set, the F1 score and accuracy of the 24-feature downsampling GBDT model were both 0.83, and the area under the curve was 0.91.

Conclusion: Compared with traditional models, artificial intelligence-based machine learning models have better accuracy and real-time performance and can reduce the occurrence of in-hospital MI from a data-driven perspective, thereby increasing the cure rate of patients and improving their prognosis.
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http://dx.doi.org/10.1186/s12859-022-04761-4DOI Listing
June 2022

The Brassicaceae Genome Resource (TBGR): a comprehensive genome platform for Brassicaceae plants.

Plant Physiol 2022 Jun 7. Epub 2022 Jun 7.

School of Life Sciences, North China University of Science and Technology, Tangshan, Hebei 063210, China.

The Brassicaceae is an important plant family. We built a user-friendly, web-based, comparative, and functional genomic database, The Brassicaceae Genome Resource (TBGR, http://www.tbgr.org.cn), based on 82 released genomes from 27 Brassicaceae species. The TBGR database contains a large number of important functional genes, including 4,096 glucosinolate genes, 6,625 auxin genes, 13,805 flowering genes, 36,632 resistance genes, 1,939 anthocyanin genes, and 1,231 m6A genes. A total of 1,174,049 specific guide sequences for CRISPR and 5,856,479 transposable elements were detected in Brassicaceae. TBGR also provides information on synteny, duplication, and orthologs for 27 Brassicaceae species. The TBGR database contains 1,183,851 gene annotations obtained using the TrEMBL, Swiss-Prot, Nr, GO, and Pfam databases. The BLAST, Synteny, Primer Design, Seq_fetch, and JBrowse tools are provided to help users perform comparative genomic analyses. All the genome assemblies, gene models, annotations, and bioinformatics results can be easily downloaded from the TBGR database. We plan to improve and continuously update the database with newly assembled genomes and comparative genomic studies. We expect the TBGR database to become a key resource for the study of the Brassicaceae.
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http://dx.doi.org/10.1093/plphys/kiac266DOI Listing
June 2022

Rare pattern of Maisonneuve fracture: A case report.

World J Clin Cases 2022 May;10(14):4684-4690

Department of Foot and Ankle Surgery Ⅰ, Tianjin Hospital, Tianjin 300211, China.

Background: Maisonneuve fracture is a special type of ankle fracture that consists of proximal fibular fracture, a lesion of the inferior tibiofibular syndesmotic complex (interosseous ligament, anterior inferior tibiofibular ligament and posterior inferior tibiofibular ligament), and injury of the medial structure of the ankle (deltoid ligament tear or medial malleolar fracture). The accepted mechanism of Maisonneuve fracture is pronation external rotation according to the Lauge-Hansen classification. In this paper, we report a rare pattern of Maisonneuve fracture, which has the characteristics of both pronation external rotation ankle fracture and supination adduction ankle fracture.

Case Summary: A 31-year-old female patient accidentally sprained her right ankle while walking 5 d before hospitalization in our hospital. The patient was initially missed in other hospitals and later rediagnosed in our outpatient department. Full-length radiographs of the lower leg revealed proximal fibula fracture, inferior tibiofibular joint separation, and medial malleolar fracture involving the posterior malleolus, which was also revealed on computed tomography scans. Magnetic resonance imaging revealed rupture of the anterior inferior tibiofibular ligament and anterior talofibular ligament. We diagnosed a rare pattern of Maisonneuve fracture with proximal fibular fracture, inferior tibiofibular joint separation, medial malleolar fracture and ruptures of the anterior inferior tibiofibular ligament and anterior talofibular ligament. The patient underwent open reduction and internal fixation in our hospital. A 6-mo postoperative follow-up confirmed a good clinical outcome.

Conclusion: To our knowledge, this rare pattern of Maisonneuve fracture has not been previously described. The possible mechanism of injury is supination adduction combined with pronation external rotation. Careful analysis of the injury mechanism of Maisonneuve fracture is of great clinical significance and can better guide clinical treatment.
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http://dx.doi.org/10.12998/wjcc.v10.i14.4684DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9125267PMC
May 2022

High-quality full-color carbon quantum dots synthesized under an unprecedentedly mild condition.

iScience 2022 Jun 18;25(6):104421. Epub 2022 May 18.

MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, Guangdong 510275, P. R. China.

Carbon quantum dots (CQDs) are highly promising to be applied in light-emitting, chemosensing, and other cutting-edge domains. Herein, we successfully fabricate high-quality full-color CQDs under unprecedentedly low temperature and pressure (85°C, 1.88 bar). Stable and narrow fluorescent emissions ranging from blue to green and red light were realized by simple amine engineering, which were further mixed into white-light CQDs with the absolute photoluminescent quantum yield reaching 19.2%. The average mass yield of the CQDs reached 69.0%. The optical performances demonstrated that the CQDs possessed uniform luminescent centers and dominant radiative decay channels. Component analysis further suggested that dehydrated condensation between carboxyl and amine groups directed the growth of the CQDs. By utilizing the CQDs, full-color light-emitting diodes and logic gate sensors were developed. This study paves an important step for promoting the application of CQDs by providing an energy-efficient, safe, and productive synthetic strategy.
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http://dx.doi.org/10.1016/j.isci.2022.104421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9157185PMC
June 2022

Protein corona-coated immunomagnetic nanoparticles with enhanced isolation of circulating tumor cells.

Nanoscale 2022 Jun 16;14(23):8474-8483. Epub 2022 Jun 16.

Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.

Immunomagnetic nanoparticles (IMNs) have been widely developed as a detection tool to isolate rare circulating tumor cells (CTCs) from whole blood as a potential method for early cancer diagnosis, metastasis examination, and treatment guidance. However, a spontaneous interaction between nanoparticles and proteins results in the formation of a protein corona that reduces the performance of IMNs when they enter body fluids. To address this issue, the protein corona was precoated onto magnetic nanoparticles (C-MNs), and then their surfaces were conjugated with an immuno-antibody. The adsorption of proteins on C-MNs was decreased 6-fold and non-specific cell binding was reduced 5-fold, compared with magnetic nanoparticles (MNs). Furthermore, the immuno-antibody functionalized C-MNs (IC-MNs) maintained highly specific CTC capture performance when exposed to blood plasma. By using artificial spiked blood samples, IC-MNs exhibited 90.2% CTC isolation efficiency, compared with 60.3% by using IMNs. IC-MNs also successfully captured CTCs with high purity in 24 out of 26 female breast cancer patient blood samples. This work demonstrated that a novel preformed protein corona strategy can provide a useful clinically applicable diagnostic tool.
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http://dx.doi.org/10.1039/d2nr01568dDOI Listing
June 2022

Natural essential oils: A promising strategy for treating cardio-cerebrovascular diseases.

J Ethnopharmacol 2022 Jun 2:115421. Epub 2022 Jun 2.

Key Laboratory of Modern Preparation of TCM, Jiangxi University of Traditional Chinese Medicine, Nanchang, China. Electronic address:

Ethnopharmacological Relevance: Essential oils (EO) are volatile compounds obtained from different parts of natural plants, and have been used in national, traditional and folk medicine to treat various health problems all over the world. Records indicate that in history, herbal medicines rich in EO have been widely used for the treatment of CVDs in many countries, such as China.

Aim Of The Study: This review focused on the traditional application and modern pharmacological mechanisms of herbal medicine EO against CVDs in preclinical and clinical trials through multi-targets synergy. Besides, the EO and anti-CVDs drugs were compared, and the broad application of EO was explained from the properties of drugs and aromatic administration routes.

Materials And Methods: Information about EO and CVDs was collected from electronic databases such as Web of Science, ScienceDirect, PubMed, and China National Knowledge Infrastructure (CNKI). The obtained data sets were sequentially arranged for better understanding of EO' potential.

Results: The study showed that EO had significant application in CVDs at different countries or regions since ancient times. Aiming at the complex pathological mechanisms of CVDs, including intracellular calcium overload, oxidative stress, inflammation, vascular endothelial cell injury and dysfunction and dyslipidemia, we summarized the roles of EO on CVDs in preclinical and clinical through multi-targets intervention. Besides, EO had the dual properties of drug and excipients. And aromatherapy was one of the complementary therapies to improve CVDs.

Conclusions: This paper reviewed the EO on traditional treatment, preclinical mechanism and clinical application of CVDs. As important sources of traditional medicines, EO' remarkable efficacy had been confirmed in comprehensive literature reports, which showed that EO had great medicinal potential.
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http://dx.doi.org/10.1016/j.jep.2022.115421DOI Listing
June 2022

Optimization via response surface methodology of the synthesis of a dust suppressant and its performance characterization for use in open cut coal mines.

J Environ Sci (China) 2022 Nov 22;121:211-223. Epub 2021 Dec 22.

College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China.

To relieve dust pollution in open cut coal mines and reduce the hazards of coal dust pollution to the environment and workers we optimized the synthesis of a dust suppressant by graft copolymerization of environmentally friendly soy protein isolate with methyl methacrylate. This dust suppressant could effectively control dust pollution in open cut coal mines. The optimized conditions for graft copolymerization in this case were determined by a response surface experiment designed with Design-Expert 10 software. Characterization by scanning electron microscopy showed a significant morphology change of the dust suppressant and the generation of a rigid and dense layer on its surface after interacting with coal dust. The layer exhibited good bonding and dust suppression performance. The analysis with Fourier-transform infrared spectroscopy revealed the appearance of new absorption peaks near 1300, 1072, and 1631 cm, demonstrating effective graft copolymerization. The proposed dust suppressant exhibited excellent wind erosion resistance, with a resistance that exceeded 90% at a wind speed of 6.5 m/sec. The successful graft copolymerization and effective bonding and curing of the dust suppressant on coal dust were experimentally verified. This is of great significance to the control of coal dust pollution.
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http://dx.doi.org/10.1016/j.jes.2021.12.006DOI Listing
November 2022

Porous interbody fusion cage design via topology optimization and biomechanical performance analysis.

Comput Methods Biomech Biomed Engin 2022 Jun 2:1-10. Epub 2022 Jun 2.

Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

The porous interbody fusion cage could provide space and stable mechanical conditions for postoperative intervertebral bone ingrowth. It is considered to be an important implant in anterior cervical discectomy and internal fixation. In this study, two types of unit cells were designed using topology optimization method and introduced to the interbody fusion cage to improve the biomechanical performances of the cage. Topology optimization under two typically loading conditions was first conducted to obtain two unit cells (O-unit cell and D-unit cell) with the same volume fraction. Porous structures were developed by stacking the obtained unit cells in space, respectively. Then, porous interbody fusion cages were obtained by the Boolean intersection between the global structural layout and the porous structures. Finite element models of cervical spine were created that C5-C6 segment was fused by the designed porous cages. The range of motion (ROM) of the cervical spine, the maximum stress on the cage and the bone graft, and the stress and displacement distributions of the cage were analyzed. The results showed the ROMs of C5-C6 segment in D-unit cell and O-unit cell models were range from 0.14° to 0.25° under different loading conditions; the cage composed of the D-unit cells had a more uniform stress distribution, smaller displacement on cage, a more reasonable internal stress transfer mode (transmission along struts of the unit cell), and higher stress on the internal bone graft (0.617 MPa). In conclusion, the optimized porous cage is a promising candidate for fusion surgery, which would avoid the cage subsidence, and promote the fusion of adjacent endplates.
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http://dx.doi.org/10.1080/10255842.2022.2081505DOI Listing
June 2022

COVID-19 Vaccine Hesitancy Among Older Adolescents and Young Adults: A National Cross-Sectional Study in China.

Front Public Health 2022 12;10:877668. Epub 2022 May 12.

Department of Neurology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Background: With promotion of COVID-19 vaccinations, there has been a corresponding vaccine hesitancy, of which older adolescents and young adults represent groups of particular concern. In this report, we investigated the prevalence and reasons for vaccine hesitancy, as well as potential risk factors, within older adolescents and young adults in China.

Methods: To assess these issues, an online survey was administered over the period from March 14 to April 15, 2021. Older adolescents (16-17 years old) and young adults (18-21 years old) were recruited nationwide from Wechat groups and results from a total of 2,414 respondents were analyzed. Socio-demographic variables, vaccine hesitancy, psychological distress, abnormal illness behavior, global well-being and social support were analyzed in this report.

Results: Compared to young adults ( = 1,405), older adolescents ( = 1,009) showed higher prevalence rates of COVID-19 vaccine hesitancy (16.5 vs. 7.9%, < 0.001). History of physical diseases ( = 0.007) and abnormal illness behavior ( = 0.001) were risk factors for vaccine hesitancy among older adolescents, while only a good self-reported health status ( = 0.048) was a risk factor for young adults. Concerns over COVID-19 vaccine side effects (67.1%) and beliefs of invulnerability regarding infection risk (41.9%) were the most prevalent reasons for vaccine hesitancy. Providing evidence on the vaccine reduction of COVID-19 infection risk (67.5%), ensuring vaccine safety (56.7%) and the low risk of side effects (52.7%) were the most effective persuasions for promoting vaccinations.

Conclusion: In China, older adolescents showed a higher prevalence for vaccine hesitancy than that of young adults. Abnormal illness behavior and history of physical diseases were risk factors for vaccine hesitancy among these older adolescents, while social support represents an important factor which could help to alleviate this hesitancy.
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http://dx.doi.org/10.3389/fpubh.2022.877668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9133905PMC
June 2022
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