Publications by authors named "Nan Chen"

1,002 Publications

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Ovarian germ cell tumor/mastocytosis with KIT mutation: a unique clinicopathological entity.

Genes Chromosomes Cancer 2021 Sep 23. Epub 2021 Sep 23.

Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Most tumors are sporadic and originated from somatic mutations. Some rare germline mutations cause familial tumors, often involving multiple tissues or organs. Tumors from somatic mosaicism during embryonic development are extremely rare. We describe here a pediatric patient who developed both an ovarian germ cell tumor and systemic mastocytosis. Targeted DNA NGS analysis revealed similar genomic changes including the same KIT D816V mutation in both tissues, suggesting a common progenitor cancer cell. The KIT mutated cells are likely from early embryonic development during germ cell migration. A literature search found additional 8 similar cases. These diseases are characterized by pediatric-onset, all-female, neoplastic proliferation in both gonad and bone marrow, and a common oncogenic cause, i.e., KIT mutation, constituting a clinically and genetically homogenous disease entity. Importantly, the association of germ cell tumors with hematopoietic neoplasms suggests that the primordial germ cells are the primitive hematopoietic stem cells, a much-debated and unsettled question. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/gcc.23000DOI Listing
September 2021

A Scalable-Bandwidth Extended State Observer-Based Adaptive Sliding-Mode Control for the Dissolved Oxygen in a Wastewater Treatment Process.

IEEE Trans Cybern 2021 Sep 20;PP. Epub 2021 Sep 20.

Strong nonlinearities, uncertainties, and disturbances present great challenges to the control of the dissolved oxygen (DO) in a wastewater treatment process (WWTP). To deal with those undesired issues, in this article, a scalable-bandwidth extended state observer (SESO) is proposed, and the SESO-based adaptive sliding-mode control (ASMC) is designed. By the SESO, the time-varying total disturbance can be estimated more accurately and compensated more effectively. For the disturbances that are not addressed completely, an ASMC is employed to suppress them. Due to the advantages of both SESO and ASMC, the DO can be regulated more desirably. The benchmark simulation model Number 1 is taken to verify the proposed SESO-based ASMC. Comparative simulation results highlight the advantages of the proposed approach.
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http://dx.doi.org/10.1109/TCYB.2021.3108166DOI Listing
September 2021

Hypophosphatemia is an independent risk factor for AKI among hospitalized patients with COVID-19 infection.

Ren Fail 2021 Dec;43(1):1329-1337

Department of Nephrology, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, China.

Background: This study sought to investigate incidence and risk factors for acute kidney injury (AKI) in hospitalized COVID-19.

Methods: In this retrospective study, we enrolled 823 COVID-19 patients with at least two evaluations of renal function during hospitalization from four hospitals in Wuhan, China between February 2020 and April 2020. Clinical and laboratory parameters at the time of admission and follow-up data were recorded. Systemic renal tubular dysfunction was evaluated 24-h urine collections in a subgroup of 55 patients.

Results: In total, 823 patients were enrolled (50.5% male) with a mean age of 60.9 ± 14.9 years. AKI occurred in 38 (40.9%) ICU cases but only 6 (0.8%) non-ICU cases. Using forward stepwise Cox regression analysis, we found eight independent risk factors for AKI including decreased platelet level, lower albumin level, lower phosphorus level, higher level of lactate dehydrogenase (LDH), procalcitonin, C-reactive protein (CRP), urea, and prothrombin time (PT) on admission. For every 0.1 mmol/L decreases in serum phosphorus level, patients had a 1.34-fold (95% CI 1.14-1.58) increased risk of AKI. Patients with hypophosphatemia were likely to be older and with lower lymphocyte count, lower serum albumin level, lower uric acid, higher LDH, and higher CRP. Furthermore, serum phosphorus level was positively correlated with phosphate tubular maximum per volume of filtrate (TmP/GFR) (Pearson  = 0.66,  < .001) in subgroup analysis, indicating renal phosphate loss proximal renal tubular dysfunction.

Conclusion: The AKI incidence was very low in non-ICU patients as compared to ICU patients. Hypophosphatemia is an independent risk factor for AKI in patients hospitalized for COVID-19 infection.
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http://dx.doi.org/10.1080/0886022X.2021.1979039DOI Listing
December 2021

The regulatory relationship and function of LncRNA FAM225A-miR-206-ADAM12 in gastric cancer.

Am J Transl Res 2021 15;13(8):8632-8652. Epub 2021 Aug 15.

Cancer Center, Taikang Xianlin Drum Tower Hospital, Nanjing University School of Medicine Nanjing, Jiangsu, China.

Objective: To investigate the role and functions of FAM225A in gastric cancer.

Methods: The expressions of FAM225A, miR-206, ADAM12 and epithelial-mesenchymal transition (EMT)-related genes were detected by quantitative real-time PCR and Western blot. Functional experiments including cell counting kit-8, colony formation, wound-healing, and Transwell assays were conducted to analyze the biological characteristics of gastric cancer cells in different groups. Bioinformatics, dual-luciferase reporter assay and Pearson correlation coefficients were performed for determining the regulatory relationship of lncRNA-miRNA-mRNA. nude mouse xenografts and immunohistochemistry were used to verify the results .

Results: In gastric cancer, FAM225A and ADAM12 expressions were up-regulated, while miR-206 expression was down-regulated. Opposite to the regulatory effects of overexpressed FAM225A, blocking FAM225A expression reduced cell viability, migration, invasion and number of cell clones, increased E-Cadherin expression, inhibited N-Cadherin and Vimentin expressions, and ultimately promoted tumor growth. MiR-206 inhibitor partially offset the effects of siFAM225A. Moreover, FAM225A competitively bound to miR-206 to up-regulate ADAM12 expression. Overexpressed ADAM12 partially reversed the effect of miR-206 mimic on the biological characteristics of gastric cancer cells and EMT-related proteins.

Conclusion: Our research revealed that FAM225A-miR-206-ADAM12 axis may be a potential pathway for regulating gastric cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430187PMC
August 2021

Jianpi Qushi Heluo Formula alleviates renal damages in Passive Hemann nephritis in rats by upregulating Parkin-mediated mitochondrial autophagy.

Sci Rep 2021 Sep 15;11(1):18338. Epub 2021 Sep 15.

China Department of Nephrology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, 100091, China.

Jianpi Qushi Heluo Formula (JQHF) is an empirical traditional Chinese medicine prescription for treating Membranous Nephropathy (MN) clinically in China. The therapeutic effect of JQHF has been reported in our previous studies. However, the exact mechanism is still unknown. In this study, by establishing an experimental rat model of MN induced by Sheep anti-rat Fx1A serum, we evaluated the effects of JQHF and Tetrandrine (TET), and Benazepril was used as a positive control. As an autophagy agonist, TET is one of the most active components in JQHF. After 4 weeks, significant kidney damage was observed in the rats in the Model group; comparatively, JQHF markedly decreased 24 h urinary protein, Total Cholesterol (TC), and increased serum total Albumin (ALB). Histology showed that JQHF caused significant improvements in glomerular hyperplasia, renal tubular damage, IgG immune complex deposition, and the ultrastructure of mitochondria in MN rats. Flow cytometry analysis showed that treatment with JQHF reduced the level of reactive oxygen species and apoptosis rate, and upregulated mitochondrial membrane potential. Western blot analysis demonstrated that JQHF could protect against mitochondrial dysfunction and apoptosis by upregulating the expression of PINK1, Mitochondrial Parkin, and LC3-II/I, downregulating the expression of Cytoplasmic Parkin, P62, Cytochrome c, and Caspase-3 in the kidneys of MN rats. From images of co-immunofluorescence, it is observed significantly increase in the co-localization of PINK1 and Parkin, as well as LC3 and mitochondria. Similarly, TET treatment significantly upregulated the mitochondrial autophagy and reduced apoptosis in rats after 4 weeks compared with the model group. Comparatively, the ability of JQHF to alleviate renal damage was significantly higher than those of Benazepril and TET. It was demonstrated that JQHF could delay pathology damage to the kidney and hold back from the progression of MN by inhibiting apoptosis and upregulating the mitochondrial autophagy by PINK1/Parkin pathways.
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http://dx.doi.org/10.1038/s41598-021-97137-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443625PMC
September 2021

Microbial response and adaption to thallium contamination in soil profiles.

J Hazard Mater 2021 Aug 30;423(Pt A):127080. Epub 2021 Aug 30.

Key Laboratory of Water Quality and Conservation in the Pearl River Delta, Ministry of Education, Guangdong Provincial Key Laboratory of Radionuclides Pollution Control and Resources, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, China; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu, China.

Thallium (Tl) is a trace metal with high toxicity. Comprehensive investigation of spatial distribution of Tl and microorganism is still limited in soils from mining area. In this study, 16S rRNA sequencing and network analysis were used for deciphering the co-occurrence patterns of bacterial communities in two different types of soil profiles around a typical Tl-bearing pyrite mine. The results showed that geochemical parameters (such as pH, S, Tl, Fe and TOM) were the driving forces for shaping the vertical distribution of microbial community. According to network analysis, a wide diversity of microbial modules were present in both soil profiles and affected by depth, significantly associated with variations in Tl geochemical fractionation. Phylogenetic information further unveiled that the microbial modules were mainly dominated by Fe reducing bacteria (FeRB), Fe oxidizing bacteria (FeOB), S oxidizing bacteria and Mn reducing bacteria. The results of metagenome indicated that Fe, Mn and S cycle in soil are closely involved in the biogeochemical cycle of Tl. The findings of co-occurrence patterns in the bacterial network and correlation between microorganisms and different geochemical fractions of Tl may benefit the strategy of bioremediation of Tl-contaminated soils with indigenous microbes.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127080DOI Listing
August 2021

Evidence Based Social Science in China Paper 2: The quality of social science RCTs published from 2000-2020.

J Clin Epidemiol 2021 Sep 11. Epub 2021 Sep 11.

Evidence Based Social Science Research Center, School of Public Health, Lanzhou University, Lanzhou, China; Evidence Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China; WHO Collaborating Centre for Guideline Implementation and Knowledge Translation, Lanzhou University, Lanzhou, China; Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, Lanzhou, China. Electronic address:

Objective: This study collected randomized controlled trials (RCTs) published in the social sciences in China and assessed their risk of bias and reporting quality.

Study Design And Setting: Three databases were systematically searched for publications from January 2000 to June 2020 for RCTs in the social sciences published by Chinese researchers. The risk of bias was assessed using the Cochrane Collaboration Risk of Bias Tool (CCRBT), and reporting quality was assessed using the Consolidated Standards of Reporting Trials for Social and Psychological Interventions (CONSORT-SPI).

Results: A total of 316 RCTs were identified, including 204 articles in English and 112 articles in Chinese. The most frequently researched interventions focused on education (33.9%), and the most frequently studied population were students (32.9%). Eighty-seven percent of RCTs had intermediate reporting quality. Twenty-four of the 43 CONSORT-SPI sub-items had a compliance rate of less than 50%. Most RCTs had an unclear risk of bias for blinding outcome assessors (84.5%), blinding participants and personnel (82.9%), allocation concealment (73.1%), and random sequence generation (68.0%). A low proportion of CONSORT-SPI items were reported and, high proportion of the papers had unclear risk of bias.

Conclusion: The quality and reporting of RCTs in the social sciences needs improvement in China, especially for reporting methods and results. Most studies had an unclear risk of bias as they lacked important methodological information.
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http://dx.doi.org/10.1016/j.jclinepi.2021.09.014DOI Listing
September 2021

Using random forest to detect multiple inherited metabolic diseases simultaneously based on GC-MS urinary metabolomics.

Talanta 2021 Dec 19;235:122720. Epub 2021 Jul 19.

Yuncheng Maternal and Child Health Hospital, Yuncheng, Shanxi, 044000, PR China.

Inborn errors of metabolism, also known as inherited metabolic diseases (IMDs), are related to genetic mutations and cause corresponding biochemical metabolic disorder of newborns and even sudden infant death. Timely detection and diagnosis of IMDs are of great significance for improving survival of newborns. Here we propose a strategy for simultaneously detecting six types of IMDs via combining GC-MS technique with the random forest algorithm (RF). Clinical urine samples from IMD and healthy patients are analyzed using GC-MS for acquiring metabolomics data. Then, the RF model is established as a multi-classification tool for the GC-MS data. Compared with the models built by artificial neural network and support vector machine, the results demonstrated the RF model has superior performance of high specificity, sensitivity, precision, accuracy, and matthews correlation coefficients on identifying all six types of IMDs and normal samples. The proposed strategy can afford a useful method for reliable and effective identification of multiple IMDs in clinical diagnosis.
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http://dx.doi.org/10.1016/j.talanta.2021.122720DOI Listing
December 2021

EAD-Net: A Novel Lesion Segmentation Method in Diabetic Retinopathy Using Neural Networks.

Dis Markers 2021 1;2021:6482665. Epub 2021 Sep 1.

The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, 646000, China.

Diabetic retinopathy (DR) is a common chronic fundus disease, which has four different kinds of microvessel structure and microvascular lesions: microaneurysms (MAs), hemorrhages (HEs), hard exudates, and soft exudates. Accurate detection and counting of them are a basic but important work. The manual annotation of these lesions is a labor-intensive task in clinical analysis. To solve the problem, we proposed a novel segmentation method for different lesions in DR. Our method is based on a convolutional neural network and can be divided into encoder module, attention module, and decoder module, so we refer it as EAD-Net. After normalization and augmentation, the fundus images were sent to the EAD-Net for automated feature extraction and pixel-wise label prediction. Given the evaluation metrics based on the matching degree between detected candidates and ground truth lesions, our method achieved sensitivity of 92.77%, specificity of 99.98%, and accuracy of 99.97% on the e_ophtha_EX dataset and comparable AUPR (Area under Precision-Recall curve) scores on IDRiD dataset. Moreover, the results on the local dataset also show that our EAD-Net has better performance than original U-net in most metrics, especially in the sensitivity and F1-score, with nearly ten percent improvement. The proposed EAD-Net is a novel method based on clinical DR diagnosis. It has satisfactory results on the segmentation of four different kinds of lesions. These effective segmentations have important clinical significance in the monitoring and diagnosis of DR.
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http://dx.doi.org/10.1155/2021/6482665DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8429028PMC
September 2021

Trait and state alterations in excitatory connectivity between subgenual anterior cingulate cortex and cerebellum in patients with current and remitted depression.

Psychiatry Res Neuroimaging 2021 Aug 8;317:111356. Epub 2021 Aug 8.

Gansu Provincial Key Laboratory of Wearable Computing, School of Information Science and Engineering, Lanzhou University, Lanzhou, PR China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, PR China. Electronic address:

Neuroimaging studies have indicated that the altered functional connectivity (FC) of the subgenual anterior cingulate cortex (sgACC) might be potential pathophysiology of major depressive disorder (MDD). However, directed connectivity is proven to be more closely to neurophysiological processes underlying brain activity than FC. This study aimed to identify the alterations underlying directed connectivity of the sgACC in patients with current and remitted MDD. We conducted a cross-sectional neuroimaging study by recruiting 36 patients with current MDD, 20 patients with remitted MDD, and 36 matched healthy controls. Multiple linear regression was employed to estimate bidirectional connectivity between bilateral sgACC and 115 brain regions over 230 time points. Besides, graph theory was applied to further investigate the information transfer across bilateral sgACC and abnormal brain regions. We found that both patients with current and remitted MDD showed a similar abnormality in bidirectional excitatory connectivity between the left sgACC and the right cerebellum. Patients with current MDD exhibited an increase in excitatory connectivity from the left cerebellum to the right sgACC, which was positively correlated with the HAMD score. Meanwhile, significantly decreased betweenness of the left sgACC was detected in all depressive patients. Our findings suggest that the changed bidirectional excitatory connectivity between the left sgACC and the right cerebellum might be a trait alteration and the abnormal increased excitatory connectivity from the left cerebellum to the right sgACC might be a state alteration of MDD. This work may provide a valuable contribution to identify trait and state alterations in the brain for depression.
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http://dx.doi.org/10.1016/j.pscychresns.2021.111356DOI Listing
August 2021

Dose-reduced trastuzumab deruxtecan can be safely used in liver failure and active leptomeningeal metastases.

Curr Probl Cancer Case Rep 2020 Dec 16;2. Epub 2020 Oct 16.

Baylor College of Medicine, Department of Internal Medicine, Section of Hematology-Oncology, One Baylor Plaza, Houston, TX, United States.

Trastuzumab deruxtecan has been shown to have responses in heavily pretreated patients with human epidermal growth factor receptor 2 (HER2)-positive metastatic breast cancer. However, the safety of this medication in patients with severe liver dysfunction and untreated or symptomatic central nervous system metastases is unknown. We describe a patient with metastatic HER2-positive breast cancer with liver failure and leptomeningeal metastases who was treated with dose-reduced trastuzumab deruxtecan. With treatment, the patient's hyperbilirubinemia resolved and she demonstrated a response on imaging. She was dose-escalated to full dose with minimal adverse events.
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http://dx.doi.org/10.1016/j.cpccr.2020.100034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8425325PMC
December 2020

Recovery of cortical atrophy in patients with temporal lobe epilepsy after successful anterior temporal lobectomy.

Epilepsy Behav 2021 Sep 6;123:108272. Epub 2021 Sep 6.

Department of Radiology and Nuclear Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, PR China; Beijing Key Laboratory of Magnetic Resonance Imaging and Brain Informatics, Beijing 100053, PR China. Electronic address:

The aims of this study were to investigate whether the cortical atrophy caused by temporal lobe epilepsy (TLE) was reversible after successful anterior temporal lobectomy (ATL) and to further observe whether possible changes are related to age at surgery and cognitive changes. Twelve patients with unilateral mesial TLE who received ATL and remained seizure free in one year follow-up were included. They underwent two MRI scans few days before and oneyear after surgery. Thirty age- and sex-matched healthy participants were recruited as controls. Group comparisons were used to test the differences in cortical thickness (CTh) between the pre-/postsurgical patients and controls. Longitudinal test was used to directly show postsurgical changes of the patients. Besides, the correlations between regional cortical volume (CVo) changes and age at surgery or cognitive changes were also tested. Compared with controls, the patients with TLE showed dispersed cortical thinning especially in the bilateral frontal lobes before surgery and no significant cortical thinning except for cortices near the resected areas after surgery. The longitudinal analysis showed CTh increment in the ipsilateral precentral and postcentral gyrus, cuneus and widespread in the contralateral cortex. In the volumetric analysis, the CVo changes in the contralateral hemisphere were negatively correlated with age at surgery and positively correlated with MoCA score changes. This study suggests that the cortical atrophy caused by TLE could recover after successful ATL. The recovery ability is greater in younger subjects and is positively related to cognitive recovery. These findings could serve as new clues that patients with TLE can benefit from timely and successful ATL.
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http://dx.doi.org/10.1016/j.yebeh.2021.108272DOI Listing
September 2021

Aorta smooth muscle-on-a-chip reveals impaired mitochondrial dynamics as a therapeutic target for aortic aneurysm in bicuspid aortic valve disease.

Elife 2021 09 6;10. Epub 2021 Sep 6.

Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Bicuspid aortic valve (BAV) is the most common congenital cardiovascular disease in general population and is frequently associated with the development of thoracic aortic aneurysm (TAA). There is no effective strategy to intervene with TAA progression due to an incomplete understanding of the pathogenesis. Insufficiency of NOTCH1 expression is highly related to BAV-TAA, but the underlying mechanism remains to be clarified.

Methods: A comparative proteomics analysis was used to explore the biological differences between non-diseased and BAV-TAA aortic tissues. A microfluidics-based aorta smooth muscle-on-a-chip model was constructed to evaluate the effect of NOTCH1 deficiency on contractile phenotype and mitochondrial dynamics of human aortic smooth muscle cells (HAoSMCs).

Results: Protein analyses of human aortic tissues showed the insufficient expression of NOTCH1 and impaired mitochondrial dynamics in BAV-TAA. HAoSMCs with NOTCH1-knockdown exhibited reduced contractile phenotype and were accompanied by attenuated mitochondrial fusion. Furthermore, we identified that mitochondrial fusion activators (leflunomide and teriflunomide) or mitochondrial fission inhibitor (Mdivi-1) partially rescued the disorders of mitochondrial dynamics in HAoSMCs derived from BAV-TAA patients.

Conclusions: The aorta smooth muscle-on-a-chip model simulates the human pathophysiological parameters of aorta biomechanics and provides a platform for molecular mechanism studies of aortic disease and related drug screening. This aorta smooth muscle-on-a-chip model and human tissue proteomic analysis revealed that impaired mitochondrial dynamics could be a potential therapeutic target for BAV-TAA.

Funding: National Key R and D Program of China, National Natural Science Foundation of China, Shanghai Municipal Science and Technology Major Project, Shanghai Science and Technology Commission, and Shanghai Municipal Education Commission.
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http://dx.doi.org/10.7554/eLife.69310DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451027PMC
September 2021

Vertical Channels Design for Polymer Electrolyte to Enhance Mechanical Strength and Ion Conductivity.

ACS Appl Mater Interfaces 2021 Sep 3;13(36):42957-42965. Epub 2021 Sep 3.

Beijing Key Laboratory of Environmental Science and Engineering, School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China.

Polymer electrolytes are the key candidates for solid-state batteries benefiting from their intrinsic advantages over inorganic electrolytes in terms of flexibility and easy processability. However, they suffer from low ionic conductivity and poor mechanical strength, which restrict their wide utilization. Conventional ceramic fillers are used to improve the mechanical properties of polymer electrolytes but lack sufficient Li conductivity. In this work, a framework with vertical channels that possess fast Li movement is designed. It is found that the poly(ethylene oxide) (PEO) compound in the vertical channel framework benefits to improve the ionic conductivity and mechanical strength synchronously. The framework in which ionic liquids are loaded on a zirconium dioxide surface ([email protected]) helps to improve ionic conductivity by 2 orders of magnitude compared with PEO, which is due to the enhanced orientation of ion transport. By optimizing the content of [email protected], the elastic modulus is also tripled. Therefore, the symmetric lithium battery can cycle stably for more than 800 h at a current density of 0.25 mA cm, whereas the lithium metal battery has a specific capacity of 135 mAh g at a current density of 2C and can cycle stably for more than 200 cycles at 60 °C.
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http://dx.doi.org/10.1021/acsami.1c13834DOI Listing
September 2021

[Changes of the upper airway in children with Class Ⅱ mandibular retrusion and snoring during night before and after functional treatment by sagittal-guidance Twin-block appliance].

Shanghai Kou Qiang Yi Xue 2021 Jun;30(3):273-277

Department of Orthodontics, Nantong Stomatological Hospital. Nantong 226000, Jiangsu Province, China. E-mail:

Purpose: To study the changes of dimension and morphology of upper airway in children with ClassⅡ mandibular retrusion after functional treatment by sagittal-guidance Twin-block appliance.

Methods: Cone-beam CT (CBCT) data of upper airway of the subjects were measured by Dolphin 11.5 software and Mimics 17.0 software , and the changes of dimension and morphology of upper airway before and after functional treatment with sagittal-guidance Twin-block(SGTB) appliance were compared. SPSS 16.0 software package was used for data processing.

Results: After functional treatment,the volume of total upper airway,nasopharynx airway, oropharynx airway, the sectional area of tip of the epiglotti(TE), the lateral diameter of TE, the base of the epiglottis(EB) significantly increased (P<0.05) in children with SGTB appliance.

Conclusions: SGTB functional treatment is effective in the treatment of skeletal ClassⅡ mandibular retrusion of children by increase of the upper airway and improvement of respiration..
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June 2021

Apatinib inhibits the proliferation of gastric cancer cells via the AKT/GSK signaling pathway .

Aging (Albany NY) 2021 08 27;13(16):20738-20747. Epub 2021 Aug 27.

Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210000, China.

Gastric cancer (GC) is the third leading cause of cancer-associated mortality globally. Although the diagnosis and therapeutic strategies for GC have improved, the prognosis for advanced gastric cancer (AGC) remains poor. Hence, the present study sought to design a zebrafish model established by microinjecting human MGC-803 GC cell line for studying personalized molecular-targeted cancer therapy. Apatinib, a novel molecular-targeted agent, was evaluated for its efficacy through a comparison among the control groups (no treatment) and subject groups (treatment). Newly formed vessel length and tumor volume were measured in all of the groups for further study. The length of newly formed vessels was obviously shortened after apatinib treatment in the zebrafish model established in this study. Meanwhile, apatinib exhibited the best antitumor growth effect with dose and time dependence by suppressing AKT/GSK3α/β signaling, which may be the mechanism underlying the profound antitumor clinical effect of apatinib. The data indicated that apatinib therapy exerts an anti-angiogenesis effect and it can be recommended as a proper antitumor growth therapy for GC patients. Additionally, zebrafish models could be designed as a potential practical tool to explore new anti-GC cancer drugs.
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http://dx.doi.org/10.18632/aging.203458DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436942PMC
August 2021

Electrocautery vs. Stapler in Comparing Safety for Segmentectomy of Lung Cancer: A Meta-Analysis.

Front Surg 2021 4;8:711685. Epub 2021 Aug 4.

Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China.

Electrocautery and staplers are regarded as the two most common surgical instruments for dissecting the intersegmental plane in segmentectomy. We performed a meta-analysis to compare electrocautery and staplers in terms of their safety and effects. A systematic search strategy was performed using PubMed, and the retrieval time was up to April 1, 2020. Odds ratio (OR) and mean differences (MDs) with 95% CI were applied to determine the effectiveness of dichotomous or continuous variables, respectively. Six studies including 385 patients were included. The electrocautery had a higher incidence rate of postoperative complication [OR= 1.92, 95% CI (1.12, 3.28), = 0.02)] and air leak [OR: 3.91, 95% CI (1.64, 9.35), = 0.002)]. No significant difference was found in the comparison of surgery time, blood loss, and duration of tube days or hospitality days. Our study indicated that patients under segmentectomy were associated with better safety by using stapler than electrocautery in the reduction of postoperative complications.
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http://dx.doi.org/10.3389/fsurg.2021.711685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371473PMC
August 2021

Exploring the variation of black and brown carbon during COVID-19 lockdown in megacity Wuhan and its surrounding cities, China.

Sci Total Environ 2021 Oct 4;791:148226. Epub 2021 Jun 4.

State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China; Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Absorbing carbonaceous aerosols, i.e. black and brown carbon (BC and BrC), affected heavily on climate change, regional air quality and human health. The nationwide lockdown measures in 2020 were performed to against the COVID-19 outbreak, which could provide an important opportunity to understand their variations on light absorption, concentrations, sources and formation mechanism of carbonaceous aerosols. The BC concentration in Wuhan megacity (WH) was 1.9 μg m during lockdown, which was 24% lower than those in the medium-sized cities and 26% higher than those in small city; in addition, 39% and 16-23% reductions occurred compared with the same periods in 2019 in WH and other cities, respectively. Fossil fuels from vehicles and industries were the major contributors to BC; and compared with other periods, minimum contribution (64-86%) mainly from fossil fuel to BC occurred during the lockdown in all cities. Secondary BrC (BrCsec) played a major role in the BrC light absorption, accounting for 65-77% in WH during different periods. BrCsec was promoted under high humidity, and decreased through the photobleaching of chromophores under higher Ox. Generally, the lockdown measures reduced the BC concentrations significantly; however, the variation of BrCsec was slight.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148226DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176899PMC
October 2021

Denitrification performance and mechanism of biofilter constructed with sulfur autotrophic denitrification composite filler in engineering application.

Bioresour Technol 2021 Nov 31;340:125699. Epub 2021 Jul 31.

School of Water Resources and Environment, China University of Geosciences (Beijing), No. 29 Xueyuan Road, Haidian District, Beijing 100083, China. Electronic address:

Sulfur autotrophic denitrification (SAD) is a promising technology due to its low cost and low sludge production. Based on previous studies on SAD materials as well as the denitrification mechanism of SAD technology, this study constructed two biofilters with a sulfur autotrophic denitrification composite filler (SADCF) to investigate the application potential of SAD technology. The feasibility of a SADCF-based biofilter was demonstrated, with a maximum nitrate volume load of 0.75 kg N/(m·d) and low accumulation of nitrite and ammonium. In addition, an improved backwashing method (air-water backwashing) was obtained by comparing two different backwashing methods. Furthermore, some iron reducing bacteria (0.4% Geothrix) along with a rapid proliferation of the main sulfur-oxidizing bacteria (23.0% Thiobacillus and 27.7% Ferritrophicum) were found under real-world operating conditions. Overall, the results of this study provide a case reference for the operation of SADCF-based biofilters and the application of SAD technology in engineering.
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http://dx.doi.org/10.1016/j.biortech.2021.125699DOI Listing
November 2021

Altered temporal reachability highlights the role of sensory perception systems in major depressive disorder.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Aug 11;112:110426. Epub 2021 Aug 11.

Gansu Provincial Key Laboratory of Wearable Computing, School of Information Science and Engineering, Lanzhou University, Lanzhou, China; Joint Research Center for Cognitive Neurosensor Technology of Lanzhou University and Institute of Semiconductors, Chinese Academy of Sciences, Lanzhou, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; Ministry of Education, Engineering Research Center of Open Source Software and Real-Time System (Lanzhou University), Lanzhou, China. Electronic address:

Background: The latest studies have considered the time-dependent structures in dynamic brain networks. However, the effect of periphery structures on the temporal flow of information remains unexplored in patients with major depressive disorder (MDD). In this work, we aimed to explore the pattern of interactions between brain regions in MDD across space and time.

Methods: We concentrated on the temporal reachability of nodes in temporal brain networks derived from the resting-state functional magnetic resonance imaging (rs-fMRI) of 55 MDD patients and 62 sex-, age-matched healthy controls. Specifically, temporal connectedness and temporal efficiency (TEF) were estimated based on the length of temporal paths between node pairs. Subsequently, the temporal clustering coefficient (TCC) and temporal distance were jointly employed to explore the patterns in which a node's periphery structure affects its reachability.

Results: Significantly higher TEF and lower TCC were found in temporal brain networks in MDD. Besides, significant between-group differences of nodal TCC were detected in regions of sensory perception systems. Considering the temporal paths that begin or end at these regions, MDD patients showed several altered temporal distances.

Conclusion: Our results showed that the temporal reachability of specific brain regions in MDD could be affected as their periphery structures evolve, which may explain the dysfunction of sensory perception systems in the spatiotemporal domain.
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http://dx.doi.org/10.1016/j.pnpbp.2021.110426DOI Listing
August 2021

Involvement of a FAD-linked oxidase RSc0454 for expression of the type III secretion system and pathogenicity in Ralstonia solanacearum.

Mol Plant Microbe Interact 2021 Aug 10. Epub 2021 Aug 10.

Southwest University, 26463, College of Resources and Environment, Southwest University, Chongqing, China, Chongqing, Sichuan, China.

Ralstonia solanacearum RSc0454 is predicated as a FAD-linked oxidase based on protein homologies, while containing distinct domains of LDH and SDH. Current study demonstrates RSc0454 exhibits LDH activity and is essential for pathogenicity. Here, we characterized involvement of RSc0454 on bacterial growth and expression of the T3SS in R. solanacearum. RSc0454 mutant grew normally in rich medium but grew faintly in host plants, and failed to grow in minimal medium. Supplementary succinate, but not lactate, substantially restored some phenotypes of RSc0454 mutants, including faint growth in plants, diminished growth in minimal medium, and lost pathogenicity. The T3SS Expression is directly controlled by a master regulator HrpB, and HrpG and PrhG positively regulate hrpB expression in parallel ways. Deletion of RSc0454 substantially reduced expression levels of hrpB and T3SS both in vitro and in planta. Moreover, RSc0454 is revealed to be required for the T3SS expression via HrpG and PrhG, but through novel pathway, and impaired expression of these genes was not due to growth deficiency of RSc0454 mutants. RSc0454 is suggested to be important for redox balance inside cells and supplementary NADH partially restored diminished growth of RSc0454 mutant in minimal medium at presence of succinate at some moderate concentrations, indicating that unbalanced redox in RSc0454 mutant might be responsible for its no growth in minimal medium. All taken together, these results provide novel insights into understanding of various biological functions of this FAD-linked oxidase RSc0454 and involvement of the redox balance on expression of the T3SS in R. solanacearum.
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http://dx.doi.org/10.1094/MPMI-07-21-0168-SCDOI Listing
August 2021

Cholinergic receptor nicotinic beta 3 subunit polymorphisms and smoking in male Chinese patients with schizophrenia.

EC Psychol Psychiatr 2021 28;10(7):11-23. Epub 2021 Jun 28.

Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06520, USA.

Objective: The aim of this study was to examine if cholinergic receptor nicotinic beta 3 subunit () was a common genetic basis for both nicotine dependence and schizophrenia.

Methods: Two promoter single nucleotide polymorphisms (SNPs) were genotyped in 773 patients with schizophrenia and 302 healthy volunteers. Associations between smoking, schizophrenia, smoking+schizophrenia and were analyzed. The mRNA expression of in human brains was examined, and the expression correlations between and dopaminergic and GABAergic receptor genes were evaluated.

Results: The association between and smoking was significant in the total sample, less significant in the smoking with schizophrenia, and suggestive in the smoking without schizophrenia. had significant mRNA expression that was correlated with dopaminergic or GABAergic receptor expression in human brains. The two SNPs had significant cis-acting regulatory effects on mRNA expression.

Conclusions: Risk for smoking behavior was associated with . mRNA is abundant in human brain and could play important role in the pathogenesis of smoking behavior.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341072PMC
June 2021

Efficacy of low-load resistance training combined with blood flow restriction vs. high-load resistance training on sarcopenia among community-dwelling older Chinese people: study protocol for a 3-arm randomized controlled trial.

Trials 2021 Aug 4;22(1):518. Epub 2021 Aug 4.

School of Kinesiology, Shanghai University of Sport, Shanghai, 200438, China.

Background: Sarcopenia is accompanied by a decline in muscle mass, muscle strength, and muscle function. Resistance training is the most potential training method for the prevention and treatment of sarcopenia. However, the conventional high-load resistance training (CRT) recommended by the American College of Sports Medicine is a challenge for older people with sarcopenia. As a novel training method, low-load resistance training combined with blood flow restriction (LRT-BFR) may elicit similar muscle mass and muscle strength gains as CRT but with less effort. The objectives of this study are to assess and compare the efficacy and safety of 12-week LRT-BFR and CRT on muscle strength, muscle performance, body composition, pulmonary function, blood biomarkers, CVD risk factors, and quality of life in community-dwelling older Chinese people with sarcopenia.

Method: This is a 12-week, assessor-blinded, 3-arm randomized controlled trial with a non-exercise control group. Community-dwelling people over 65 years will be screened for sarcopenia according to the diagnostic criteria of the Asian Working Group for Sarcopenia (AWGS). Fifty-one subjects will be randomized into a LRT-BFR group (n = 17), a CRT group (n = 17), and a no-strength training control group (n = 17). The primary outcome is lower limb muscle strength. The secondary outcomes are body composition, upper limb muscle strength, pulmonary function, blood biomarkers, CVD risk factors, and quality of life. Post-intervention follow-up will be performed for 12 weeks. These indicators will be assessed at baseline (0 week), after the 12-week intervention (12 weeks), and at follow-up (24 weeks). The adverse events will also be reported. Data will be analyzed for all participants in an intent-to-treat plan.

Discussion: This study is the first RCT that will systematically measure and compare the efficacy and safety of LRT-BFR and CRT in older people with sarcopenia on muscle strength, body composition, pulmonary function, blood biomarkers (inflammatory biomarkers, hormone, and growth factors), CVD risk factors, and quality of life. This study can provide an efficient and safe method to prevent the progression of sarcopenia in older people.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2100042803 . Registered on 28 January 2021.
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http://dx.doi.org/10.1186/s13063-021-05495-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8336391PMC
August 2021

Differences in Facial Expression Recognition Between Unipolar and Bipolar Depression.

Front Psychol 2021 14;12:619368. Epub 2021 Jul 14.

Peking University HuiLongGuan Clinical Medical School, Beijing Huilongguan Hospital, Beijing, China.

Purpose: To explore the differences in facial emotion recognition among patients with unipolar depression (UD), bipolar depression (BD), and normal controls.

Methods: Thirty patients with UD and 30 patients with BD, respectively, were recruited in Zhumadian Second People's Hospital from July 2018 to August 2019. Fifteen groups of facial expressions including happiness, sadness, anger, surprise, fear, and disgust were identified.

Results: A single-factor ANOVA was used to analyze the facial expression recognition results of the three groups, and the differences were found in the happy-sad ( = 0.009), happy-angry ( = 0.001), happy-surprised ( = 0.034), and disgust-surprised ( = 0.038) facial expression groups. The independent sample -test analysis showed that compared with the normal control group, there were differences in the happy-sad ( = 0.009) and happy-angry ( = 0.009) groups in patients with BD, and the accuracy of facial expression recognition was lower than the normal control group. Compared with patients with UD, there were differences between the happy-sad ( = 0.005) and happy-angry ( = 0.002) groups, and the identification accuracy of patients with UD was higher than that of patients with BD. The time of facial expression recognition in the normal control group was shorter than that in the patient group. Using happiness-sadness to distinguish unipolar and BDs, the area under the ROC curve (AUC) is 0.933, the specificity is 0.889, and the sensitivity is 0.667. Using happiness-anger to distinguish unipolar and BD, the AUC was 0.733, the specificity was 0.778, and the sensitivity was 0.600.

Conclusion: Patients with UD had lower performance in recognizing negative expressions and had longer recognition times. Those with BD had lower accuracy in recognizing positive expressions and longer recognition times. Rapid facial expression recognition performance may be as a potential endophenotype for early identification of unipolar and BD.
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http://dx.doi.org/10.3389/fpsyg.2021.619368DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8316620PMC
July 2021

Characteristics and Clinical Outcomes of 295 Patients With Relapsing Polychondritis.

Authors:
Nan Chen Yi Zheng

J Rheumatol 2021 Aug 1. Epub 2021 Aug 1.

N. Chen, MD, Department of Rheumatology and Immunology, Beijing Tongren Hospital, Capital Medical University; Y. Zheng, MD, Department of Rheumatology and Immunology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. The authors declare no conflicts of interest relevant to this article. Address correspondence to Dr. Y. Zheng, Department of Rheumatology and Immunology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China. Email: Accepted for publication July 16, 2021.

Objective: This study analyzes the clinical features of Chinese patients with relapsing polychondritis (RP).

Methods: The clinical data of 295 patients with RP at Beijing Tongren Hospital were retrospectively analyzed.

Results: The mean age of onset was 41.0 ± 15.0 years. The sex ratio was 1:1. Up to 70.5% of the patients had airway involvement during the disease course; among them, the larynx was most commonly affected (82.2%). One-quarter (25.7%) of the patients with laryngeal involvement underwent tracheotomy as a result of progressive dyspnea or acute laryngeal obstruction. Younger age at onset and respiratory symptoms at initial presentation were independent risk factors for tracheotomy in patients with RP with laryngeal involvement. The risk of tracheotomy in patients who presented with respiratory symptoms was 2.35 times higher than that of patients who presented with other symptoms (HR 2.35, 95% CI 1.23-4.50, = 0.01). The risk of tracheotomy increased by 4.8% for every 1-year decrease in the age at onset (HR 0.95, 95% CI 0.93-0.97, < 0.001). The incidence of lower respiratory tract infection was much higher in patients with airway involvement than in those without airway involvement. The main cause of death was respiratory failure as a result of airway obstruction.

Conclusion: There is a high prevalence of airway involvement in Chinese patients with RP. Laryngeal involvement is associated with a high risk of death. More attention should be paid to patients with RP with laryngeal involvement who are young at disease onset and present with respiratory symptoms.
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http://dx.doi.org/10.3899/jrheum.210062DOI Listing
August 2021

Oxygen vacancies-enriched CoFeO for peroxymonosulfate activation: The reactivity between radical-nonradical coupling way and bisphenol A.

J Hazard Mater 2021 Sep 8;418:126357. Epub 2021 Jun 8.

College of Resources and Environment, University of Chinese Academy of Sciences, Huaibei Town 380, Huairou District, Beijing 101408, China; State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address:

Oxygen vacancies (O) play a vital role in catalytic activity. Herein, a series of MOF-derived CoFeO nanomaterials with O tuned by a simple thermal aging strategy are prepared for peroxymonosulfate (PMS) activation. Remarkably, the stability, structural and catalytic properties show dependence on the annealing temperature. The abundant surface O and functional groups on CoFeO were verified as active sites to boost catalytic activity. Based on the density functional theory (DFT) calculations, (1 1 1), (2 2 2) and (4 2 2) planes exposed at higher temperatures facilitate catalytic performance, ascribed to the intense surface adsorption energy. The quenching and electron paramagnetic resonance (EPR) experiments indicate catalysis degradation is a radical-nonradical coupling process. The reactivity between reactive oxygen species (ROS) and bisphenol A and the radical-nonradical dual degradation pathways are systematically explored by combined DFT and HPLC-MS.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126357DOI Listing
September 2021

The choline metabolite TMAO inhibits NETosis and promotes placental development in GDM of humans and mice.

Diabetes 2021 Jul 27. Epub 2021 Jul 27.

Department of Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China

Choline metabolite Trimethylamine N-oxide (TMAO) has been recognized as a risk factor of gestational diabetes mellitus (GDM), but its exact role in GDM has not been reported. In this study, we focused on the placenta development to reveal the role of TMAO in GDM. We found the TMAO levels in peripheral and cord plasma were increased in women with GDM, and TMAO levels were positively correlated with newborn weight and placental thickness. Neutrophil extracellular traps (NETs) in the peripheral and cord plasma and the myeloperoxidase expression in the placenta of women with GDM also increased. NETs could inhibit the proliferation, migration, invasion and angiogenesis of HTR-8/Svneo cells. However, TMAO could not only inhibit the formation of NETs, but also enhance the biological function of HTR-8/Svneo cells. By inducing GDM models in NETs deficient PAD4 and wild-type mice, the placental weight of PAD4 mice increased significantly. TMAO feeding also inhibited the formation of NETs and further increased the weight of the placenta and fetuses, and this increase did not affect the placental structure. Our data indicated that higher TMAO levels and the formation of abnormal NETs were associated with GDM. TMAO could not only promote the development of the placenta and fetuses, but also inhibit the formation of NETs.
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http://dx.doi.org/10.2337/db21-0188DOI Listing
July 2021

Modulation of fatty acid elongation in cockroaches sustains sexually dimorphic hydrocarbons and female attractiveness.

PLoS Biol 2021 Jul 27;19(7):e3001330. Epub 2021 Jul 27.

State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi, China.

Insect cuticular hydrocarbons (CHCs) serve as important intersexual signaling chemicals and generally show variation between the sexes, but little is known about the generation of sexually dimorphic hydrocarbons (SDHCs) in insects. In this study, we report the molecular mechanism and biological significance that underlie the generation of SDHC in the German cockroach Blattella germanica. Sexually mature females possess more C29 CHCs, especially the contact sex pheromone precursor 3,11-DimeC29. RNA interference (RNAi) screen against the fatty acid elongase family members combined with heterologous expression of the genes in yeast revealed that both BgElo12 and BgElo24 were involved in hydrocarbon (HC) production, but BgElo24 is of wide catalytic activities and is able to provide substrates for BgElo12, and only the female-enriched BgElo12 is responsible for sustaining female-specific HC profile. Repressing BgElo12 masculinized the female CHC profile, decreased contact sex pheromone level, and consequently reduced the sexual attractiveness of female cockroaches. Moreover, the asymmetric expression of BgElo12 between the sexes is modulated by sex differentiation cascade. Specifically, male-specific BgDsx represses the transcription of BgElo12 in males, while BgTra is able to remove this effect in females. Our study reveals a novel molecular mechanism responsible for the formation of SDHCs and also provide evidences on shaping of the SDHCs by sexual selection, as females use them to generate high levels of contact sex pheromone.
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http://dx.doi.org/10.1371/journal.pbio.3001330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315507PMC
July 2021

Cuticular Hydrocarbon Plasticity in Three Rice Planthopper Species.

Int J Mol Sci 2021 Jul 20;22(14). Epub 2021 Jul 20.

Institute of Insect Science, Zhejiang University, Hangzhou 310058, China.

Insect cuticular hydrocarbons (CHCs) are organic compounds of the surface lipid layer, which function as a barrier against water loss and xenobiotic penetration, while also serving as chemical signals. Plasticity of CHC profiles can vary depending upon numerous biological and environmental factors. Here, we investigated potential sources of variation in CHC profiles of , and which are considered to be the most important rice pests in Asia. CHC profiles were quantified by GC/MS, and factors associated with variations were explored by conducting principal component analysis (PCA). Transcriptomes were further compared under different environmental conditions. The results demonstrated that CHC profiles differ among three species and change with different developmental stages, sexes, temperature, humidity and host plants. Genes involved in cuticular lipid biosynthesis pathways are modulated, which might explain why CHC profiles vary among species under different environments. Our study illustrates some biological and ecological variations in modifying CHC profiles, and the underlying molecular regulation mechanisms of the planthoppers in coping with changes of environmental conditions, which is of great importance for identifying potential vulnerabilities relating to pest ecology and developing novel pest management strategies.
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http://dx.doi.org/10.3390/ijms22147733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304831PMC
July 2021

[Quantitation and content investigation of bilirubin in cultured cow-bezoar].

Zhongguo Zhong Yao Za Zhi 2021 Jun;46(11):2852-2856

National Institutes for Food and Drug Control Beijing 102629, China.

A determination method for bilirubin in cultured cow-bezoar was developed in this study, with which the bilirubin in 15 batches of samples was quantified. The samples were first processed with 10% oxalic acid solution for the conversion of bilirubin from conjugated to unconjugated, followed by the extraction with dichloromethane. Then the obtained sample solutions were analyzed at 450 nm by HPLC[chromatographic column: Agilent TC-C_(18)(4.6 mm × 250 mm, 5 μm); mobile phase: acetonitrile and 1% glacial acetic acid aqueous solution(95∶5); flow rate: 1.0 mL·min~(-1)]. The bilirubin content in the 15 batches of cultured cow-bezoar was ranged from 21.9% to 41.7% with the average of 32.4%. The proposed method is accurate and reliable, thus making it suitable for the quantitation of bilirubin in cultured cow-bezoar and its quality assessment and control.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20210305.202DOI Listing
June 2021
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