Publications by authors named "Na Zhao"

986 Publications

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis.

J Inflamm Res 2022 28;15:4315-4329. Epub 2022 Jul 28.

Department of Medical Laboratory, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China.

Objective: Mannan-binding lectin (MBL), a soluble pattern recognition molecule of the innate immune system, is primarily synthesized in the liver and secreted into the circulation. Low serum level of MBL has been reported to be related to an increased risk of lung diseases. Herein, we aimed to investigate the function of MBL in silicosis-associated pulmonary inflammation.

Methods: Serum collected from silicosis patients was tested for correlation between serum MBL levels and Th17 immunity. In vitro studies were performed to further demonstrated the effect of MBL on Th17 polarization. Silica was intratracheally injected in wild type (WT) or MBL-deficient (MBL) mice to induce silicosis-associated lung inflammation and fibrosis. Th17 response was evaluated to explore the effect of MBL on silicosis in vivo.

Results: Silicosis patients with high serum MBL levels displayed ameliorative lung function. We demonstrated that serum MBL levels negatively correlated to Th17 cell frequency in silicosis patients. MBL protein markedly reduced expression of IL-17 but enhanced expression of Foxp3 in CD4 T cells in vitro when subjected to Th17 or Treg polarizing conditions, respectively. The presence of MBL during Th17 cell polarization significantly limited aryl hydrocarbon receptor (AhR) expression and suppressed the signal transducer and activator of transcription 3 (STAT3) phosphorylation. Treatment with the AhR antagonist abolished the effect of MBL on Th17 response. Strikingly, MBL directly bound to AhR and affected its nuclear translocation. Furthermore, MBL mice displayed elevated Th17 cell levels compared with WT mice in response to the silica challenge. The CD4 T lymphocytes from silica-administrated MBL mice exhibited more AhR expression than the wild-type counterparts.

Conclusion: Our study suggested that MBL limited the Th17 immunity via controlling the AhR/STAT3 pathway, thus providing new insight into silicosis and other inflammatory diseases in patients with MBL deficiency.
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http://dx.doi.org/10.2147/JIR.S357453DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342710PMC
July 2022

The Gut Microbiota Determines the High-Altitude Adaptability of Tibetan Wild Asses () in Qinghai-Tibet Plateau.

Front Microbiol 2022 18;13:949002. Epub 2022 Jul 18.

Northwest Institute of Plateau Biology and Institute of Sanjiangyuan National Park, Chinese Academy of Sciences, Xining, China.

It was acknowledged long ago that microorganisms have played critical roles in animal evolution. Tibetan wild asses (TWA, ) are the only wild perissodactyls on the Qinghai-Tibet Plateau (QTP) and the first national protected animals; however, knowledge about the relationships between their gut microbiota and the host's adaptability remains poorly understood. Herein, 16S rRNA and meta-genomic sequencing approaches were employed to investigate the gut microbiota-host associations in TWA and were compared against those of the co-resident livestock of yak () and Tibetan sheep (). Results revealed that the gut microbiota of yak and Tibetan sheep underwent convergent evolution. By contrast, the intestinal microflora of TWA diverged in a direction enabling the host to subsist on sparse and low-quality forage. Meanwhile, high microbial diversity (Shannon and Chao1 indices), cellulolytic activity, and abundant indicator species such as Spirochaetes, Bacteroidetes, , and supported forage digestion and short-chain fatty acid production in the gut of TWA. Meanwhile, the enterotype identification analysis showed that TWA shifted their enterotype in response to low-quality forage for a better utilization of forage nitrogen and short-chain fatty acid production. Metagenomic analysis revealed that plant biomass degrading microbial consortia, genes, and enzymes like the cellulolytic strains (, and ), as well as carbohydrate metabolism genes (GH43, GH3, GH31, GH5, and GH10) and enzymes (β-glucosidase, xylanase, and β-xylosidase, etc.) had a significantly higher enrichment in TWA. Our results indicate that gut microbiota can improve the adaptability of TWA through plant biomass degradation and energy maintenance by the functions of gut microbiota in the face of nutritional deficiencies and also provide a strong rationale for understanding the roles of gut microbiota in the adaptation of QTP wildlife when facing harsh feeding environments.
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http://dx.doi.org/10.3389/fmicb.2022.949002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342865PMC
July 2022

Peripheral apoE4 enhances Alzheimer's pathology and impairs cognition by compromising cerebrovascular function.

Nat Neurosci 2022 Aug 1;25(8):1020-1033. Epub 2022 Aug 1.

Department of Neuroscience, Mayo Clinic, Jacksonville, FL, USA.

The ε4 allele of the apolipoprotein E (APOE) gene, a genetic risk factor for Alzheimer's disease, is abundantly expressed in both the brain and periphery. Here, we present evidence that peripheral apoE isoforms, separated from those in the brain by the blood-brain barrier, differentially impact Alzheimer's disease pathogenesis and cognition. To evaluate the function of peripheral apoE, we developed conditional mouse models expressing human APOE3 or APOE4 in the liver with no detectable apoE in the brain. Liver-expressed apoE4 compromised synaptic plasticity and cognition by impairing cerebrovascular functions. Plasma proteome profiling revealed apoE isoform-dependent functional pathways highlighting cell adhesion, lipoprotein metabolism and complement activation. ApoE3 plasma from young mice improved cognition and reduced vessel-associated gliosis when transfused into aged mice, whereas apoE4 compromised the beneficial effects of young plasma. A human induced pluripotent stem cell-derived endothelial cell model recapitulated the plasma apoE isoform-specific effect on endothelial integrity, further supporting a vascular-related mechanism. Upon breeding with amyloid model mice, liver-expressed apoE4 exacerbated brain amyloid pathology, whereas apoE3 reduced it. Our findings demonstrate pathogenic effects of peripheral apoE4, providing a strong rationale for targeting peripheral apoE to treat Alzheimer's disease.
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http://dx.doi.org/10.1038/s41593-022-01127-0DOI Listing
August 2022

Fabrication of Gold-Based "Sphere-on-Plate" Hybrid Nanostructures with Dual Plasmonic Absorptions Covering Visible and Near-Infrared II Windows the Volmer-Weber Growth Mode.

Langmuir 2022 Aug 25;38(31):9669-9677. Epub 2022 Jul 25.

School of Chemistry, Chemical Engineering, and Materials, Jining University, Qufu 273155, Shandong, China.

We report a synthetic strategy to create gold(Au)-based "sphere-on-plate" hybrid nanostructures (SPHNSs). The surface doping of plate-like Au seeds with Pt/Ag atoms is found to be crucial to increase the lattice spacing, inducing island-like deposition of Au atoms the Volmer-Weber growth mode. The resulting products are featured with the morphology that quasi-spherical nanoparticles are scattered over the nanoplates. Due to the presence of two distinctly dimensioned particles in one entity, the current Au-based SPHNSs exhibit unique dual plasmonic absorptions, where the visible absorbance centered at 546 nm is related to the size of the anchored particles. Arising from such a plasmonic advantage, the Au-based SPHNSs exhibit enhancement in photothermal conversion under laser irradiations at the wavelengths of both 808 and 1064 nm. The current work offers a feasible route to fabricate noble metal hybrid nanostructures involving zero-dimensional (0D) and two-dimensional (2D) structures, which could work as promising materials for photothermal conversion.
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http://dx.doi.org/10.1021/acs.langmuir.2c01289DOI Listing
August 2022

Exploration of the Immunotyping Landscape and Immune Infiltration-Related Prognostic Markers in Ovarian Cancer Patients.

Front Oncol 2022 8;12:916251. Epub 2022 Jul 8.

Department of Cancer Research, Hanyu Biomed Center Beijing, Beijing, China.

Background: Increasing evidence indicates that immune cell infiltration (ICI) affects the prognosis of multiple cancers. This study aims to explore the immunotypes and ICI-related biomarkers in ovarian cancer.

Methods: The ICI levels were quantified with the CIBERSORT and ESTIMATE algorithms. The unsupervised consensus clustering method determined immunotypes based on the ICI profiles. Characteristic genes were identified with the Boruta algorithm. Then, the ICI score, a novel prognostic marker, was generated with the principal component analysis of the characteristic genes. The relationships between the ICI scores and clinical features were revealed. Further, an ICI signature was integrated after the univariate Cox, lasso, and stepwise regression analyses. The accuracy and robustness of the model were tested by three independent cohorts. The roles of the model in the immunophenoscores (IPS), tumor immune dysfunction and exclusion (TIDE) scores, and immunotherapy responses were also explored. Finally, risk genes (GBP1P1, TGFBI, PLA2G2D) and immune cell marker genes (CD11B, NOS2, CD206, CD8A) were tested by qRT-PCR in clinical tissues.

Results: Three immunotypes were identified, and ICI scores were generated based on the 75 characteristic genes. CD8 TCR pathways, chemokine-related pathways, and lymphocyte activation were critical to immunophenotyping. Higher ICI scores contributed to better prognoses. An independent prognostic factor, a three-gene signature, was integrated to calculate patients' risk scores. Higher TIDE scores, lower ICI scores, lower IPS, lower immunotherapy responses, and worse prognoses were revealed in high-risk patients. Macrophage polarization and CD8 T cell infiltration were indicated to play potentially important roles in the development of ovarian cancer in the clinical validation cohort.

Conclusions: Our study characterized the immunotyping landscape and provided novel immune infiltration-related prognostic markers in ovarian cancer.
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http://dx.doi.org/10.3389/fonc.2022.916251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307664PMC
July 2022

Analysis of Intervention Effect and Satisfaction of Holistic Nursing after Oral Tumor Resection.

Comput Math Methods Med 2022 15;2022:3788605. Epub 2022 Jul 15.

Department of Oral and Maxillofacial Surgery, Zhangqiu District People's Hospital, Jinan 250200, China.

Objective: To explore the intervention effect and satisfaction analysis of holistic nursing after oral tumor resection.

Methods: A total of 70 oral tumor patients who underwent surgical treatment in our hospital from April 2020 to September 2021 were randomly divided into two groups, with 35 patients in each group. The control group was given basic oral care, the observation group was given overall oral care, and the actual effects of the two groups of care were compared, including the emotional status, compliance and nursing satisfaction, hospital stay and nursing quality scores, pain level, quality of life, and complications occurred.

Results: After nursing, the patients in the observation group had good mood, higher compliance and nursing satisfaction, shorter hospital stay, higher nursing quality and quality of life scores, lower pain level, and lower incidence of complications, when compared with the control group.

Conclusion: Holistic nursing has obvious effects on patients after oral tumor surgery, which can relieve patients' negative emotions, improve patients' compliance with treatment, improve their quality of life, and effectively reduce the degree of pain and the occurrence of complications, which is worthy of clinical promotion.
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http://dx.doi.org/10.1155/2022/3788605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307384PMC
July 2022

Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma.

Front Microbiol 2022 4;13:913718. Epub 2022 Jul 4.

Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Different gut microbiota is implicated in different diseases, including cancer. However, gut microbiota differences between individuals with clear cell renal cell carcinoma (ccRCC) and healthy individuals are unclear. Here, we analyzed gut microbiota composition in 51 ccRCC patients and 40 healthy controls using 16S rRNA sequencing analysis. We observed that , , , , and were dominant and positively associated with ccRCC. We isolated and cultured to characterize specific gut microbiota that promotes ccRCC and found that it promoted ccRCC proliferation, migration, and invasion the TGF-signaling pathway. Interactions identified between the gut microbiota and ccRCC suggest the gut microbiota could serve as a potential non-invasive tool for predicting ccRCC risk and also function as a cancer therapy target.
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http://dx.doi.org/10.3389/fmicb.2022.913718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295744PMC
July 2022

Comprehensive narrative review of real-world COVID-19 vaccines: viewpoints and opportunities.

Med Rev (Berl) 2022 Apr 25;2(2):169-196. Epub 2022 May 25.

Guangdong Provincial Centre for Disease Control and Prevention, Guangzhou, Guangdong Province, China.

Currently, people all over the world have been affected by coronavirus disease 2019 (COVID-19). Fighting against COVID-19 is the top priority for all the countries and nations. The development of a safe and effective COVID-19 vaccine is considered the optimal way of ending the pandemic. Three hundred and 44 vaccines were in development, with 149 undergoing clinical research and 35 authorized for emergency use as to March 15 of 2022. Many studies have shown the effective role of COVID-19 vaccines in preventing SARS-CoV-2 infections as well as serious and fatal COVID-19 cases. However, tough challenges have arisen regarding COVID-19 vaccines, including long-term immunity, emerging COVID-19 variants, and vaccine inequalities. A systematic review was performed of recent COVID-19 vaccine studies, with a focus on vaccine type, efficacy and effectiveness, and protection against SARS-CoV-2 variants, breakthrough infections, safety, deployment and vaccine strategies used in the real-world. Ultimately, there is a need to establish a unified evaluation standard of vaccine effectiveness, monitor vaccine safety and effectiveness, along with the virological characteristics of SARS-CoV-2 variants; and determine the most useful booster schedule. These aspects must be coordinated to ensure timely responses to beneficial or detrimental situations. In the future, global efforts should be directed toward effective and immediate vaccine allocations, improving vaccine coverage, SARS-CoV-2 new variants tracking, and vaccine booster development.
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http://dx.doi.org/10.1515/mr-2021-0021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274757PMC
April 2022

Predicting Divorce Prospect Using Ensemble Learning: Support Vector Machine, Linear Model, and Neural Network.

Comput Intell Neurosci 2022 11;2022:3687598. Epub 2022 Jul 11.

School of Computing Engineering and Physical Sciences, University of West of Scotland, Paisley, UK.

A divorce is a legal step taken by married people to end their marriage. It occurs after a couple decides to no longer live together as husband and wife. Globally, the divorce rate has more than doubled from 1970 until 2008, with divorces per 1,000 married people rising from 2.6 to 5.5. Divorce occurs at a rate of 16.9 per 1,000 married women. According to the experts, over half of all marriages ends in divorce or separation in the United States. A novel ensemble learning technique based on advanced machine learning algorithms is proposed in this study. The support vector machine (SVM), passive aggressive classifier, and neural network (MLP) are applied in the context of divorce prediction. A question-based dataset is created by the field specialist. The responses to the questions provide important information about whether a marriage is likely to turn into divorce in the future. The cross-validation is applied in 5 folds, and the performance results of the evaluation metrics are examined. The accuracy score is 100%, and Receiver Operating Characteristic (ROC) curve accuracy score, recall score, the precision score, and the F1 accuracy score are close to 97% confidently. Our findings examined the key indicators for divorce and the factors that are most significant when predicting the divorce.
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http://dx.doi.org/10.1155/2022/3687598DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293523PMC
July 2022

Mechanisms of high-level fosfomycin resistance in Staphylococcus aureus epidemic lineage ST5.

J Antimicrob Chemother 2022 Jul 15. Epub 2022 Jul 15.

Department of Laboratory Medicine, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.

Objectives: Fosfomycin resistance has become a clinical concern. In this study, we analysed the dynamic change of fosfomycin MIC in the epidemic Staphylococcus aureus lineages in a teaching hospital in Shanghai for 12 years and sought to elucidate the major underlying mechanisms.

Methods: MLST was conducted for 4580 S. aureus isolates recovered from 2008 to 2019. Fosfomycin MIC was determined by the agar dilution method. The genome data of 230 S. aureus epidemic lineage isolates were acquired from a next-generation sequencing (NGS) platform. Gene deletion and corresponding complementation mutants were constructed to confirm the mechanism of fosfomycin resistance.

Results: The predominant S. aureus lineages during the past 12 years were ST5 and ST239 (45.6%; 2090/4580). However, ST5 has been spreading clinically, while ST239 has gradually disappeared recently. Consistent with epidemic trends, fosfomycin-resistant ST5 increased from 19.5% to 67.3%. Most fosfomycin-resistant ST5 isolates (92.7%; 647/698) possessed high-level resistance (MIC > 1024 mg/L) with combined mutations mainly in glpT and uhpT. In contrast, fosfomycin-resistant ST239 isolates (76.8%; 149/194) mainly acquired low-level resistance (MIC = 64-128 mg/L) with mutation primarily in hptA. Deletion of a single resistant gene merely resulted in low-level fosfomycin resistance, while double-gene mutants ΔglpTΔuhpT, ΔglpTΔhptA and ΔglpTΔhptR acquired high-level fosfomycin resistance.

Conclusions: The high-level fosfomycin resistance of S. aureus epidemic lineage ST5 is mainly due to the accumulation of mutations in the resistant genes related to membrane transporter systems, and partly contributes to its persistent prevalence under clinical antibiotic pressure.
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http://dx.doi.org/10.1093/jac/dkac236DOI Listing
July 2022

The Response of Ruminal Microbiota and Metabolites to Different Dietary Protein Levels in Tibetan Sheep on the Qinghai-Tibetan Plateau.

Front Vet Sci 2022 29;9:922817. Epub 2022 Jun 29.

Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China.

Ruminal microbiota and metabolites play crucial roles in animal health and productivity. Exploring the dynamic changes and interactions between microbial community composition and metabolites is important for understanding ruminal nutrition and metabolism. Tibetan sheep () are an important livestock resource on the Qinghai-Tibetan Plateau (QTP), and the effects of various dietary protein levels on ruminal microbiota and metabolites are still unknown. The aim of this study was to investigate the response of ruminal microbiota and metabolites to different levels of dietary protein in Tibetan sheep. Three diets with different protein levels (low protein 10.1%, medium protein 12.1%, and high protein 14.1%) were fed to Tibetan sheep. 16S rRNA gene sequencing and gas chromatography coupled with time-of-flight mass spectrometry (GC-TOF-MS) were used to study the profile changes in each group of ruminal microbes and metabolites, as well as the potential interaction between them. The rumen microbiota in all groups was dominated by the phyla Bacteroidetes and Firmicutes regardless of the dietary protein level. At the genus level, and were dominant. Under the same forage-to-concentrate ratio condition, the difference in the dietary protein levels had no significant impact on the bacterial alpha diversity index and relative abundance of the major phyla and genera in Tibetan sheep. Rumen metabolomics analysis revealed that dietary protein levels altered the concentrations of ruminal amino acids, carbohydrates and organic acids, and significantly affected tryptophan metabolism ( < 0.05). Correlation analysis of the microbiota and metabolites revealed positive and negative regulatory mechanisms. Overall, this study provides detailed information on rumen microorganisms and ruminal metabolites under different levels of dietary protein, which could be helpful in subsequent research for regulating animal nutrition and metabolism through nutritional interventions.
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http://dx.doi.org/10.3389/fvets.2022.922817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277223PMC
June 2022

The Diagnostic Value of Combined Detection of Serum Lp-PLA2 and Hcy and Color Doppler in Elderly Patients with Acute Coronary Syndrome and Effect on Endothelial Function.

Evid Based Complement Alternat Med 2022 8;2022:3150670. Epub 2022 Jul 8.

Cangzhou Central Hospital, Cangzhou, Hebei, China.

Objective: To evaluate the application value of serum lipoprotein-associated phospholipase A2 (Lp-PLA2) and homocysteine (Hcy) combined with color Doppler detection in elderly patients with acute coronary syndrome (ACS) and the influence on their endothelial function.

Methods: From February 2019 to October 2020, 100 elderly patients with suspected ACS treated in our hospital were recruited and assigned (1 : 1) to a control group or an experimental group. The control group received color Doppler detection, and the experimental group received the combined detection of serum Lp-PLA2 and Hcy and color Doppler. Positive results were determined by the combined detection of the levels of LP-PLA2 and Hcy and color Doppler. The two groups were compared in terms of specificity, susceptibility, positive detection rate, negative detection rate, test accuracy, the area under the receiver operating characteristic (ROC) curve (AUC), the expression levels of interleukin-2 (IL-2), interleukin-12 (IL-12), interleukin-13 (IL-13), and the vascular endothelial function. The patients with ACS were divided into three subgroups according to the Gensini score three-quartile interval, and their Lp-PLA2 levels were calculated for analysis.

Results: The test specificity, susceptibility, positive detection rate, negative detection rate, test accuracy, and AUC in the experimental group were much better than the those in the control group (all < 0.05), and there were no significant differences in the expression levels of IL-2, IL-12, IL-13, and vascular endothelial function between the two groups ( > 0.05). The three subgroups showed similar levels of fasting blood glucose, total cholesterol, triglyceride, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol, apolipoprotein A-I, glutamic-pyruvic transaminase, lutamic-oxaloacetic transaminase, homocysteine, and amino-terminal pro-brain natriuretic peptide (all > 0.05). The severe condition group had the highest Lp-PLA2 level, followed by the moderate group, and then the mild group ( < 0.05). Moreover, the levels of LP-PLA2, aspartate transaminase (AST), and N-terminal pro hormone B-type natriuretic peptide (NT-proBNP) were all influencing factors for the coronary Gensini score (all < 0.05).

Conclusion: The combined detection of serum Lp-PLA2, Hcy, and color Doppler significantly improves the diagnostic accuracy, specificity, and susceptibility in the elderly with ACS, with little impact on their vascular endothelial function.
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http://dx.doi.org/10.1155/2022/3150670DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9286968PMC
July 2022

Impact of infertility duration on male sexual function and mental health.

J Assist Reprod Genet 2022 Jul 15. Epub 2022 Jul 15.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, No.39 Huaxiang Road, Tiexi District, Shenyang, 110072, Liaoning Province, China.

Purpose: This study aimed to explore whether infertility duration has an impact on the sexual function and mental health of men from infertile couples.

Methods: We conducted a case-control study of 558 men from infertile couples, and the participants were divided into four groups based on their infertility duration: group I: ≤ 2 years; group II: 2-5 years; group III: 5-8 years; and group IV: > 8 years. Sexual function and mental disorders were measured using the International Index of Erectile Function-15 (IIEF-15), Premature Ejaculation Diagnostic Tool (PEDT), Generalized Anxiety Disorder scale (GAD-7), and Patient Health Questionnaire (PHQ-9) separately.

Results: As the years of infertility duration increase, the total IIEF-15 score and four domains (sexual desire, orgasmic function, erectile function, and intercourse satisfaction) significantly decrease (p < 0.05). The PEDT score gradually increases significantly (p < 0.05). Increased infertility duration is an independent risk factor for erectile dysfunction and premature ejaculation (p < 0.05). However, increased infertility duration is not a risk factor for depression and anxiety (p > 0.05).

Conclusions: Our study is the first time to use the infertility duration as an independent variable and group this variable to analyze its impact on the sexual function and mental health of men from infertile couples comprehensively and systematically. The increased infertility duration is an independent risk factor for the occurrence of sexual dysfunction but not for mental disorders. In the process of infertility treatment, sexual health and mental health cannot be ignored, especially for patients with prolonged infertility.
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http://dx.doi.org/10.1007/s10815-022-02550-9DOI Listing
July 2022

LncRNA NEAT1/microRNA-124 regulates cell viability, inflammation and fibrosis in high-glucose-treated mesangial cells.

Exp Ther Med 2022 Aug 9;24(2):507. Epub 2022 Jun 9.

Department of Endocrinology, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, Anhui 230031, P.R. China.

Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) has been frequently found to be dysregulated, which contributes to diabetes-related complications. The present study aimed to explore the effect of knockdown on mouse mesangial cell (MMC) viability, apoptosis, inflammation and fibrosis in an model of diabetic nephropathy (DN). The SV40 MES13 MMC cell line was first cultured with high glucose to establish an MMC DN cell model. Lnc-NEAT1 shRNA or the negative control shRNA were transfected into MMC DN cells, followed by the measurement of cell viability, apoptosis, inflammation, fibrosis and microRNA (miR)-124 expression, a known target of lnc-NEAT1, using Cell Counting Kit-8, flow cytometry, ELISA, western blotting [Capain1 (capn1), β-catenin (CTNNB1), cleaved caspase 3, cleaved poly-(ADP ribose) polymerase, fibronectin and Collagen] and reverse transcription-quantitative PCR (Capn1, CTNNB1, lnc-NEAT1, fibronectin, collagen and miR-124), respectively. In rescue experiments, the miR-124 and negative control inhibitor were co-transfected into lnc-NEAT1-downregulated cells, following which cell viability, apoptosis, inflammation, fibrosis, capn1 and CTNNB1 expression were measured. Lnc-NEAT1 expression was increased in high glucose-treated cells compared with that in normal glucose-treated cells and osmotic control cells, suggesting that lnc-NEAT1 is overexpressed in the MMC DN cell model. In the MMC DN cell model, lncRNA-NEAT1 knockdown enhanced cell apoptosis but reduced cell viability and the secretion of inflammatory cytokines in the supernatant (IL-1β, IL-8, monocyte chemotactic protein 1 and TNF-α), in addition to reducing the expression of fibrosis markers fibronectin and collagen I in the lysates. Lnc-NEAT1 knockdown increased miR-124 expression. Furthermore, transfection with the miR-124 inhibitor reduced cell apoptosis but increased cell viability, inflammation and fibrosis in lnc-NEAT1-downregulated MMC DN cells. miR-124 inhibitor transfection also increased the expression levels of Capn1 and CTNNB1. Taken together, the findings of the present study demonstrated that lnc-NEAT1 knockdown was able to attenuate MMC viability, inflammation and fibrosis by regulating miR-124 expression and the Capn1/β-catenin signaling pathway downstream. Therefore, Lnc-NEAT1 may serve as a potential therapeutic target for DN.
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http://dx.doi.org/10.3892/etm.2022.11434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257954PMC
August 2022

High-Performance Thermoelectric α-Ag Ga Te Compounds with Ultralow Lattice Thermal Conductivity Originating from Ag Te Motifs.

Angew Chem Int Ed Engl 2022 Jul 12:e202208281. Epub 2022 Jul 12.

State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, China.

We have determined the complex atomic structure of high-temperature α-Ag GaTe phase with a hexagonal lattice (P6 mc space group, a=b=8.2766 Å, c=13.4349 Å). The structure has outer [GaTe ] tetrahedrons and inner [Ag Te ] clusters. All of the Ag ions are disorderly distributed in the lattice. Seven types of the Ag atoms constitute the cage-like [Ag Te ] clusters. The highly disordered Ag ions vibrate in-harmonically, producing strong coupling between low frequency optical phonons and acoustic phonons. This in conjunction with a low sound velocity of 1354 m s leads to an ultralow thermal conductivity of 0.20 W m  K at 673 K. Meanwhile, the deficiency of Ga in Ag Ga Te compounds effectively optimizes the electronic transport properties. Ag Ga Te attains a highest power factor of 0.40 mW m  K at 673 K. All these contribute to a much-improved ZT value of 1.13 at 623 K for Ag Ga Te , which is 41 % higher than that of the pristine Ag GaTe sample.
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http://dx.doi.org/10.1002/anie.202208281DOI Listing
July 2022

Model Embraced Electromechanical Coupling Time for Estimation of Heart Failure in Patients With Hypertrophic Cardiomyopathy.

Front Cardiovasc Med 2022 16;9:895035. Epub 2022 Jun 16.

Department of Cardiovascular Medicine, Shaanxi Provincial People's Hospital, Xi'an, China.

Objective: This study aimed to establish a model embraced electromechanical coupling time (EMC-T) and assess the value of the model for the prediction of heart failure (HF) in patients with hypertrophic cardiomyopathy (HCM).

Materials And Methods: Data on 82 patients with HCM at Shaanxi Provincial People's Hospital between February 2019 and November 2021 were collected and then formed the training dataset ( = 82). Data were used to screen predictors of HF using univariate and multivariate analyses. Predictors were implemented to discover the optimal cut-off value, were incorporated into a model, and shown as a nomogram. The cumulative HF curve was calculated using the Kaplan-Meier method. Additionally, patients with HCM at other hospitals collected from March 2019 to March 2021 formed the validation dataset. The model's performance was confirmed both in training and validation sets.

Results: During a median of 22.91 months, 19 (13.38%) patients experienced HF. Cox analysis showed that EMC-T courses in the lateral wall, myoglobin, PR interval, and left atrial volume index were independent predictors of HF in patients with HCM. Five factors were incorporated into the model and shown as a nomogram. Stratification of patients into two risk subgroups by applying risk score (<230.65, ≥230.65) allowed significant distinction between Kaplan-Meier curves for cumulative incidence of HF events. In training dataset, the model had an AUC of 0.948 (95% CI: 0.885-1.000, < 0.001) and achieved a good C-index of 0.918 (95% CI: 0.867-0.969). In validation dataset, the model had an AUC of 0.991 (95% CI: 0.848-1.000, < 0.001) and achieved a strong C-index of 0.941 (95% CI: 0.923-1.000). Calibration plots showed high agreement between predicted and observed outcomes in both two datasets.

Conclusion: We established and validated a novel model incorporating electromechanical coupling time courses for predicting HF in patients with HCM.
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http://dx.doi.org/10.3389/fcvm.2022.895035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254680PMC
June 2022

Cross Analysis of Genomic-Pathologic Features on Multiple Primary Hepatocellular Carcinoma.

Front Genet 2022 20;13:846517. Epub 2022 Jun 20.

Department of Oncology, Peking University International Hospital, Beijing, China.

Hepatocellular carcinoma (HCC) is a prevalent malignancy cancer worldwide with a poor prognosis. Hepatic resection is indicated as a potentially curative option for HCC patients in the early stage. However, due to multiple nodules, it leads to clinical challenges for surgical management. Approximately 41%-75% of HCC cases are multifocal at initial diagnosis, which may arise from multicentric occurrence (MO-HCC) or intrahepatic metastasis (IM-HCC) pattern with significantly different clinical outcomes. Effectively differentiating the two mechanisms is crucial to prioritize the allocation of surgery for multifocal HCC. In this study, we collected a multifocal hepatocellular carcinoma cohort of 17 patients with a total of 34 samples. We performed whole-exome sequencing and staining of pathological HE sections for each lesion. Reconstruction of the clonal evolutionary pattern using genome mutations showed that the intrahepatic metastogenesis pattern had a poorer survival performance than independent origins, with variants in the TP53, ARID1A, and higher CNV variants occurring more significantly in the metastatic pattern. Cross-modality analysis with pathology showed that molecular classification results were consistent with pathology results in 70.6% of patients, and we found that pathology results could further complement the classification for undefined patterns of occurrence. Based on these results, we propose a model to differentiate the pattern of multifocal hepatocellular carcinoma based on the pathological results and genome mutations information, which can provide guidelines for diagnosing and treating multifocal hepatocellular carcinoma.
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http://dx.doi.org/10.3389/fgene.2022.846517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251469PMC
June 2022

Imparting pH and temperature dual-responsiveness in a micellar solution of cationic surfactants by introducing a hydrotrope.

Soft Matter 2022 Jul 20;18(28):5249-5260. Epub 2022 Jul 20.

Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, P. R. China.

Over the recent years, intelligent, multi-responsive micelles have received considerable attention due to their promising application in a variety of fields, including biomedical technology, drug delivery, separation, and catalysis. However, the design of such systems with controlled self-assembly is challenging both experimentally and theoretically and is still in the nascent stage. In this study, a novel dual-stimuli triggered wormlike micellar solution is prepared by mixing cationic surfactants 3-hexadecyloxy-2-hydroxypropyltrimethylammonium bromide (RHTAB) and sodium hydrogen phthalate (SHP). The viscoelasticity, aggregate morphology, and pH- and thermo-responsive behavior of the micellar solution are examined by rheological measurements, cryogenic-transmission electron microscopy (cryo-TEM), nuclear magnetic resonance (H NMR) spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The dual-sensitive fluid can be switched between a water-like state and a gel-like state by adjusting the pH and temperature. The variations in the flowing behavior are ascribed to the microstructural transition between wormlike micelles, short cylindrical micelles, and spherical micelles. Furthermore, based on the experimental results, dual-responsive behavior of the mixed solution is attributed to the different binding modes between SHP and the surfactant with the variation in the pH and temperature. We hope that the proposed system provides a new route for developing multi-stimuli-responsive materials that are capable of adapting to local environmental variations.
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http://dx.doi.org/10.1039/d2sm00509cDOI Listing
July 2022

Increased serum anti-CYP2E1 IgG autoantibody levels may be involved in the pathogenesis of occupational trichloroethylene hypersensitivity syndrome: a case-control study.

Arch Toxicol 2022 10 28;96(10):2785-2797. Epub 2022 Jun 28.

Department of Occupational and Environmental Health, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi, Japan.

Occupational exposure to trichloroethylene (TCE) causes a systemic skin disorder with hepatitis known as TCE hypersensitivity syndrome (TCE-HS). Human Leukocyte Antigen (HLA)-B*13:01 is its susceptibility factor; however, the immunological pathogenesis of TCE-HS remains unknown. We herein examined the hypothesis that autoantibodies to CYP2E1 are primarily involved in TCE-HS. A case-control study of 80 TCE-HS patients, 186 TCE-tolerant controls (TCE-TC), and 71 TCE-nonexposed controls (TCE-nonEC) was conducted to measure their serum anti-CYP2E1 antibody (IgG) levels. The effects of TCE exposure indices, such as 8-h time-weighted-average (TWA) airborne concentrations, urinary metabolite concentrations, and TCE usage duration; sex; smoking and drinking habits; and alanine aminotransferase (ALT) levels on the antibody levels were also analyzed in the two control groups. There were significant differences in anti-CYP2E1 antibody levels among the three groups: TCE-TC > TCE-HS patients > TCE-nonEC. Antibody levels were not different between HLA-B*13:01 carriers and noncarriers in TCE-HS patients and TCE-TC. The serum CYP2E1 measurement suggested increased immunocomplex levels only in patients with TCE-HS. Multiple regression analysis for the two control groups showed that the antibody levels were significantly higher by the TCE exposure. Women had higher antibody levels than men; however, smoking, drinking, and ALT levels did not affect the anti-CYP2E1 antibody levels. Anti-CYP2E1 antibodies were elevated at concentrations lower than the TWA concentration of 2.5 ppm for TCE exposure. Since HLA-B*13:01 polymorphism was not involved in the autoantibody levels, the possible mechanism underlying the pathogenesis of TCE-HS is that TCE exposure induces anti-CYP2E1 autoantibody production, and HLA-B*13:01 is involved in the development of TCE-HS.
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http://dx.doi.org/10.1007/s00204-022-03326-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352743PMC
October 2022

Effects of COVID-19 vaccination status, vaccine type, and vaccination interval on IVF pregnancy outcomes in infertile couples.

J Assist Reprod Genet 2022 Jun 27. Epub 2022 Jun 27.

Center of Reproductive Medicine, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, Tiexi District, Shenyang, 110072, Liaoning Province, China.

Purpose: This study aimed to explore whether the coronavirus disease (COVID-19) vaccination of both partners in infertile couples, different types of COVID-19 vaccines, and the interval between complete vaccination and oocyte retrieval or embryo transfer (ET) affect the quality of embryos and pregnancy rates in in vitro fertilization (IVF).

Methods: This was a prospective cohort study, comprising 735 infertile couples conducted between December 6, 2021, and March 31, 2022, in a single university hospital-based IVF center. The patients were divided into different groups according to the vaccination status of both partners in infertile couples, type of vaccine, and interval between complete vaccination and IVF treatment. The embryo quality and pregnancy rates were compared among different groups.

Results: The results showed that embryo quality and pregnancy rates had no significant differences among different groups. The multivariate regression model showed that the vaccination status of both infertile couples, types of vaccines, and intervals had no significant effects on the clinical pregnancy rate.

Conclusions: The vaccination status of both partners in infertile couples, different types of vaccines, and time intervals have no effect on embryo quality and pregnancy rates in IVF. This is the first study to compare the vaccination status of both partners in infertile couples and the impact of different vaccine types on pregnancy rates and embryo quality in detail. Our findings provide evidence of vaccine safety for infertile couples wishing to undergo IVF treatment. This evidence is crucial for decision-making by clinicians and policymakers involved in IVF cycles.
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http://dx.doi.org/10.1007/s10815-022-02543-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244292PMC
June 2022

The dynamics of cardiovascular and respiratory deaths attributed to long-term PM exposures in global megacities.

Sci Total Environ 2022 Jun 23;842:156951. Epub 2022 Jun 23.

Institute of Surface-Earth System Science, School of Earth System Science, Tianjin University, Tianjin, China.

Exposure to ambient fine particulate matter (PM) air pollution is a significant driver of premature deaths. We estimate the number of cardiovascular and respiratory (CR) premature deaths attributed to long-term exposure to PM in 33 global megacities based on long-term remotely sensed observations from 2000 to 2019. Our analysis uses high-resolution (0.01 degree) PM concentration data and cause-specific integrated exposure-response (IER) functions developed for the Global Burden of Disease Project. From 2000 to 2019, PM-related CR death rates per 1000 people increased in 6 of 33 megacities, decreased in 9, and remained constant in 18 megacities. The increase in PM-related CR mortality in 11 megacities located in South and East Asia during the period 2000-2019 can be attributed to the increases in PM concentrations. All 33 megacities could avoid 30,248 (9 %), 62,989 (20 %), 128,457 (40 %), 198,462 (62 %) and all of the estimated 322,515 CR deaths attributed to PM pollution in 2019 if they were to attain the World Health Organization's four interim PM targets (IT-1, IT-2, IT-3, and IT-4) and the new air quality guideline (AQG), respectively. Major improvements in air quality are needed to reduce the number of CR deaths attributed to PM in South and East Asia, in addition to ny reductions that would likely follow shifts in the population structures of these megacities moving forward.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156951DOI Listing
June 2022

Multiple roles of Runt-related transcription factor-2 in tooth eruption: bone formation and resorption.

Arch Oral Biol 2022 Sep 15;141:105484. Epub 2022 Jun 15.

Central Laboratory, Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, PR China. Electronic address:

Objective: The aim was to provide a comprehensive review of the current knowledge of the multiple roles of Runt-related transcription factor-2 (RUNX2) in regulating tooth eruption, focusing on the molecular mechanisms regarding tooth eruption mediated by RUNX2.

Design: Relevant literatures in PubMed, Medline, and Scopus database were searched, and a narrative review was performed. The multiple roles of RUNX2 in regulating tooth eruption was reviewed and discussed.

Results: Aberrant RUNX2 expression leads to disturbed or failed tooth eruption. Tooth eruption involves both the process of bone formation and bone resorption. RUNX2 promotes osteogenesis around the radicular portion of the dental follicle that provides the biological force for tooth eruption through inducing the expression of osteogenesis-related genes in dental follicle cells/osteoblasts. On the other hand, through indirect and direct pathways, RUNX2 regulates osteoclastogenesis and the formation of the eruption pathway.

Conclusion: RUNX2 exerts a pivotal and complex influence in regulating tooth eruption. This review provides a better understanding of the function of RUNX2 in tooth eruption, which is beneficial to illuminate the precise molecular mechanism of osteogenesis and bone resorption, aiding the development of effective therapy for the failure of tooth eruption.
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http://dx.doi.org/10.1016/j.archoralbio.2022.105484DOI Listing
September 2022

The pro-inflammatory effect of Staphylokinase contributes to community-associated Staphylococcus aureus pneumonia.

Commun Biol 2022 Jun 23;5(1):618. Epub 2022 Jun 23.

Department of Laboratory Medicine, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Pneumonia caused by community-associated Staphylococcus aureus (CA-SA) has high morbidity and mortality, but its pathogenic mechanism remains to be further investigated. Herein, we identify that staphylokinase (SAK) is significantly induced in CA-SA and inhibits biofilm formation in a plasminogen-dependent manner. Importantly, SAK can enhance CA-SA-mediated pneumonia in both wild-type and cathelicidins-related antimicrobial peptide knockout (CRAMP) mice, suggesting that SAK exacerbates pneumonia in a CRAMP-independent manner. Mechanistically, SAK induces pro-inflammatory effects, especially in the priming step of NLRP3 inflammasome activation. Moreover, we demonstrate that SAK can increase K efflux, production of reactive oxygen species production, and activation of NF-κB signaling. Furthermore, the NLRP3 inflammasome inhibitor can counteract the effective of SAK induced CA-SA lung infection in mice. Taken together, we speculate that SAK exacerbates CA-SA-induced pneumonia by promoting NLRP3 inflammasome activation, providing new insights into the pathogenesis of highly virulent CA-SA and emphasizes the importance of controlling inflammation in acute pneumonia.
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http://dx.doi.org/10.1038/s42003-022-03571-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226170PMC
June 2022

Angiographic Lesion Morphology Provides Incremental Value to Generalize Quantitative Flow Ratio for Predicting Myocardial Ischemia.

Front Cardiovasc Med 2022 6;9:872498. Epub 2022 Jun 6.

Department of Radiology, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Aim: The quantitative flow ratio (QFR) is favorable for functional assessment of coronary artery stenosis without pressure wires and induction of hyperemia. The aim of this study was to explore whether angiographic lesion morphology provides incremental value to generalize QFR for predicting myocardial ischemia in unselected patients.

Methods: This study was a substudy to the CT-FFR CHINA trial, referring 345 participants from five centers with suspected coronary artery disease on coronary CT angiography for diagnostic invasive coronary angiography (ICA). Fractional flow reserve (FFR) was measured in all vessels with 30-90% diameter stenosis. QFR was calculated in 186 lesions from 159 participants in a blinded manner. In addition, parameters to characterize lesion features were recorded or measured, including left anterior descending arteries (LADs)-involved lesions, side branch located at stenotic lesion (BL), multiple lesions (ML), minimal lumen diameter (MLD), reference lumen diameter (RLD), percent diameter stenosis (%DS), lesion length (LL), and LL/MLD. Logistic regression was used to construct two kinds of models by combining single or two lesion parameters with the QFR. The performances of these models were compared with that of QFR on a per-vessel level.

Results: A total of 148 participants (mean age: 59.5 years; 101 men) with 175 coronary arteries were included for final analysis. In total, 81 (46%) vessels were considered hemodynamically significant. QFR correctly classified 82.29% of the vessels using FFR with a cutoff of 0.80 as reference standard. The area under the receiver operating characteristic curve (AUC) of QFR was 0.86 with a sensitivity, specificity, positive predictive value, and negative predictive value of 80.25, 84.04, 81.25, and 83.16%, respectively. The combined models (QFR + LAD + MLD, QFR + LAD + %DS, QFR + BL + MLD, and QFR + BL + %DS) outperformed QFR with higher AUCs (0.91 vs. 0.86, = 0.02; 0.91 vs. 0.86, = 0.02; 0.91 vs. 0.86, = 0.02; 0.90 vs. 0.86, = 0.03, respectively). Compared with QFR, the sensitivity of the combined models (QFR + BL and QFR + MLD) was improved (91.36 vs. 80.25%, 91.36 vs. 80.25%, respectively, both < 0.05) without compromised specificity or accuracy.

Conclusion: Combined with angiographic lesion parameters, QFR can be optimized for predicting myocardial ischemia in unselected patients.
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http://dx.doi.org/10.3389/fcvm.2022.872498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207314PMC
June 2022

The "Hand as Foot" method for teaching placenta increta.

Asian J Surg 2022 Jun 19. Epub 2022 Jun 19.

Department of Obstetrics and Gynecology, Binzhou Medical University Hospital, Binzhou, Shandong province, 256600, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.049DOI Listing
June 2022

[Variations of δC in water-soluble compounds during spring phenology of typical tree species in the warm temperate zone].

Ying Yong Sheng Tai Xue Bao 2022 Jun;33(6):1466-1474

Institute of Forestry and Pomology, Beijing Aca-demy of Agriculture and Forestry Sciences, Beijing 100093, China.

In this study, we examined the regularity of phenological rhythmical change of plant water-soluble compound δC (δC) in spring for two typical tree species in the warm temperate zone of China, and . The δC in each organ of those two species in the spring phenological period were measured to explore the relationship between δC and related environmental factors. The results showed that there were significant differences in δC values of each organ between s and , with higher δC(-25.03‰±0.01‰) in the new shoot of . The δC value in the non-photosynthetic organs were 0.83‰-1.8‰ higher than that in the photosynthetic organs, while the δC value in the aboveground part was generally lower than that in the underground part. As spring progressing, different carbon storage strategies were found between two species. When the terminal bud of opened, the carbon was obtained from the proximal old leaves. At the beginning of leaf development, photosynthetic products accumulated by old leaves could not meet the growth requirements for new leaves and roots, with 90% of which depending on the carbon reserve in branches and stems. When full leaf having developed, the photosynthetic function of both new and old leaves recovered and the carbon consumed by branches and stems was gradually replenished. For , at the beginning of leaf bud opening and leaf spreading, branches were the main carbon source for new leaves and roots. When leaves were fully unfolded, mature leaves with high capacity of carbon sequestration became the primary carbon source. Results of principal component analysis showed that temperature during observation period, ≥10 ℃ accumulated temperature, sunshine duration and solar radiation were the main factors influencing δC, which could explained 86.3% of the total variation. The δC values of both species was negatively correlated with temperature and relative humidity, but positively correlated with the difference of saturated water pressure, ≥10 ℃ accumulated temperature and sunshine duration. The main environmental factors affecting plant δC varied during the phenological process. Our results could provide a reference for more accurate estimation of spring organ carbon distribution pattern of regional typical tree species, and also a theoretical basis for formulating scientific and reasonable forest management strategy.
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http://dx.doi.org/10.13287/j.1001-9332.202206.012DOI Listing
June 2022

Anti-N-methyl-D-aspartate receptor encephalitis: characteristics and rapid diagnostic approach in the emergency department.

BMC Neurol 2022 Jun 18;22(1):224. Epub 2022 Jun 18.

Emergency Department, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Anti-N-methyl-D-aspartate receptor (anti-NMDAR) encephalitis is a common type of autoimmune encephalitis. Patients with this condition are frequently very ill but are often misdiagnosed in the Emergency Department (ED). The objective of this study was to analyze the clinical characteristics of anti-NMDAR patients in the ED and to identify any associations with a diagnosis of anti-NMDAR encephalitis.

Methods: We performed a retrospective analysis of a prospectively obtained cohort of ED patients from May 2011 to December 2017. We identified patients diagnosed with anti-NMDAR encephalitis in this cohort and extracted key patient characteristics and clinical data, including patient gender, age, presentation, modified Rank Score (m-RS), laboratory test results, significant treatments, and mortality.

Results: Eighty-seven patients with anti-NMDAR encephalitis were identified. 54 (62.1%) were female, 23 (26.4%) were < 18 years old, 14 (16.1%) had teratoma, and 45 (51.7%) had an m-RS ≥ 4. Fever, altered mental status, and seizures were the most common symptoms, with a > 50% incidence of each symptom in the cohort. The sensitivity of CSF oligoclonal band (OB) testing was 78.9%. 22 (25.3%) were admitted to the ICU, 20 (23.0%) patients were intubated, but only one patient died (1.1%). 47 (54.0%) were misdiagnosed prior to ED arrival. All patients underwent immunotherapy as first-line treatment for anti-NMDAR encephalitis.

Conclusions: A majority of anti-NMDAR encephalitis patients presenting to the ED were female and were likely to be misdiagnosed prior to arrival. Patients with symptoms of fever, altered mental status, and seizures need a lumbar puncture, including CSF OB testing, for definitive diagnosis.
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http://dx.doi.org/10.1186/s12883-022-02752-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206331PMC
June 2022

Response of PM-bound elemental species to emission variations and associated health risk assessment during the COVID-19 pandemic in a coastal megacity.

J Environ Sci (China) 2022 Dec 18;122:115-127. Epub 2021 Oct 18.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.

The coronavirus (COVID-19) pandemic is disrupting the world from many aspects. In this study, the impact of emission variations on PM-bound elemental species and health risks associated to inhalation exposure has been analyzed based on real-time measurements at a remote coastal site in Shanghai during the pandemic. Most trace elemental species decreased significantly and displayed almost no diel peaks during the lockdown. After the lockdown, they rebounded rapidly, of which V and Ni even exceeded the levels before the lockdown, suggesting the recovery of both inland and shipping activities. Five sources were identified based on receptor modeling. Coal combustion accounted for more than 70% of the measured elemental concentrations before and during the lockdown. Shipping emissions, fugitive/mineral dust, and waste incineration all showed elevated contributions after the lockdown. The total non-carcinogenic risk (HQ) for the target elements exceeded the risk threshold for both children and adults with chloride as the predominant species contributing to HQ. Whereas, the total carcinogenic risk (TR) for adults was above the acceptable level and much higher than that for children. Waste incineration was the largest contributor to HQ, while manufacture processing and coal combustion were the main sources of TR. Lockdown control measures were beneficial for lowering the carcinogenic risk while unexpectedly increased the non-carcinogenic risk. From the perspective of health effects, priorities of control measures should be given to waste incineration, manufacture processing, and coal combustion. A balanced way should be reached between both lowering the levels of air pollutants and their health risks.
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http://dx.doi.org/10.1016/j.jes.2021.10.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8520875PMC
December 2022

A Flexible and Wearable Nylon Fiber Sensor Modified by Reduced Graphene Oxide and ZnO Quantum Dots for Wide-Range NO Gas Detection at Room Temperature.

Materials (Basel) 2022 May 25;15(11). Epub 2022 May 25.

State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Reduced graphene oxide (rGO) fiber as a carbon-based fiber sensor has aroused widespread interest in the field of gas sensing. However, the low response value and poor flexibility of the rGO fiber sensor severely limit its application in the field of flexible wearable electronics. In this paper, a flexible and wearable nylon fiber sensor modified by rGO and ZnO quantum dots (QDs) is proposed for wide-range NO gas detection at room temperature. The response value of the nylon fiber sensor to 100 ppm NO gas is as high as 0.4958, and the response time and recovery time are 216.2 s and 667.9 s, respectively. The relationship between the sensor's response value and the NO concentration value is linear in the range of 20-100 ppm, and the fitting coefficient is 0.998. In addition, the test results show that the sensor also has good repeatability, flexibility, and selectivity. Moreover, an early warning module was also designed and is proposed in this paper to realize the over-limit monitoring of NO gas, and the flexible sensor was embedded in a mask, demonstrating its great application potential and value in the field of wearable electronics.
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http://dx.doi.org/10.3390/ma15113772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181485PMC
May 2022

Short- and long-read metagenomics expand individualized structural variations in gut microbiomes.

Nat Commun 2022 06 8;13(1):3175. Epub 2022 Jun 8.

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

In-depth profiling of genetic variations in the gut microbiome is highly desired for understanding its functionality and impacts on host health and disease. Here, by harnessing the long read advantage provided by Oxford Nanopore Technology (ONT), we characterize fine-scale genetic variations of structural variations (SVs) in hundreds of gut microbiomes from healthy humans. ONT long reads dramatically improve the quality of metagenomic assemblies, enable reliable detection of a large, expanded set of structural variation types (notably including large insertions and inversions). We find SVs are highly distinct between individuals and stable within an individual, representing gut microbiome fingerprints that shape strain-level differentiations in function within species, complicating the associations to metabolites and host phenotypes such as blood glucose. In summary, our study strongly emphasizes that incorporating ONT reads into metagenomic analyses expands the detection scope of genetic variations, enables profiling strain-level variations in gut microbiome, and their intricate correlations with metabolome.
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http://dx.doi.org/10.1038/s41467-022-30857-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177567PMC
June 2022
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