Publications by authors named "Na Wang"

1,723 Publications

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Double plastic stent implantation for recurrent acute pancreatitis with incomplete pancreas divisum: a case report and literature review.

J Int Med Res 2021 Nov;49(11):3000605211060142

Department of Gastroenterology, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, 71213Second Hospital of Hebei Medical University, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China.

Pancreas divisum (PD) is a common pancreatic malformation caused by the failure of fusion between ventral and dorsal pancreatic ducts. There is a small branch of communication between the two systems in incomplete PD, and this variation has an incidence of 15%. A 43-year-old female patient presented to our department with recurrent abdominal pain. Magnetic resonance cholangiopancreatography (MRCP) showed that the ventral pancreatic duct was curved, with a local pouchlike dilatation. Endoscopic ultrasonography supported the diagnosis of incomplete PD and showed a thin branch of communication between ventral and dorsal pancreatic ducts. Endoscopic retrograde cholangiopancreatography (ERCP) and papillotomy of the minor papilla with double plastic stent implantation were performed. One pancreatic plastic stent was inserted across the minor and major papilla over the guide wire, creating a U-shape. The other wire-guided plastic stent was inserted through the minor papilla into the dorsal pancreatic duct. The pancreatic fluid drained smoothly after stent placement. During the 6-month follow-up, the patient remained well, without recurrence of pancreatitis.
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http://dx.doi.org/10.1177/03000605211060142DOI Listing
November 2021

Association between early-onset affective disorders and hypothyroidism in a larger number of psychiatric drug-free patients.

J Affect Disord 2021 Nov 25. Epub 2021 Nov 25.

Xinxiang Medical University, The Second Affiliated Hospital, Xinxiang, 453002, China; Xinxiang Medical University, School of Public Health, Xinxiang, 453003, China. Electronic address:

Aims: We aimed to assess the association between early onset of affective disorders and hypothyroidism in a larger number of psychiatric drug-free patients with bipolar disorder (BD) or major depressive disorder (MDD).

Methods: Early onset of affective disorders was defined as BD and MDD developed before the age of 22 years. The hypothyroidism hierarchy were diagnosed biochemically and included subclinical and overt hypothyroidism in this study. Demographic and clinical data including diagnosis, illness duration and thyroid function parameters (TSH, T4, FT4, T3 and FT3) at admission were collected from patients' records. Adjusted odds ratio (OR) and 95% confidence interval (CI), which were estimated by logistic regression model, were used to assess the association between early-onset affective disorder and hypothyroidism.

Results: The prevalence of hypothyroidism in early-onset affective disorders was higher than that in late-onset patients (in BD, 9.4% vs. 6.2%, χ =6.020, P=0.014; and in MDD 12.7% vs. 6.6%, χ =13.295, P < 0.001). Early-onset affective disorders were 2.097 times (95% CI: 1.409-3.123) more likely to have hypothyroidism compared with late-onset patients, after adjustment for age, gender, duration of illness and mood episode (adjusted OR: 1.965, 95% CI: 1.198-3.221 in BD, and adjusted OR: 2.831, 95% CI: 1.378-5.817 in MDD, respectively).

Limitation: Because of the cross-sectional design of this study, we were unable to sort out causality between early-onset affective disorders and hypothyroidism.

Conclusion: Early-onset affective disorder may be associated with higher prevalence of hypothyroidism.
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http://dx.doi.org/10.1016/j.jad.2021.11.061DOI Listing
November 2021

A Z-Linked E3 Ubiquitin Ligase Is Involved in Gametogenesis in Chinese Tongue Sole, .

Animals (Basel) 2021 Nov 15;11(11). Epub 2021 Nov 15.

Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Qingdao 266071, China.

Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase in () was cloned and characterized. The full-length cDNA was composed of 1962 bp, including 551 bp 5'UTR, 736 bp 3'UTR, and 675 bp ORF encoding a 224-amino-acid (aa) protein. was examined among seven different tissues and found to be predominantly expressed in gonads. In testis, could be detected from 40 days post hatching (dph) until 3 years post hatching (yph), but there was a significant increase at 6 months post hatching (mph). In comparison, the expression levels in ovary were rather stable among different developmental stages. In situ hybridization showed that was mainly localized in germ cells, that is, spermatid and spermatozoa in testis and stage I, II and III oocytes in ovary. In vitro RNA interference found that knockdown resulted in the decline of and in ovarian cell line and down-regulation of in the testicular cell line. These data suggested that might participate in gonadal differentiation and gametogenesis, via regulating steroid hormone synthesis.
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http://dx.doi.org/10.3390/ani11113265DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614299PMC
November 2021

The relationship between health literacy and health-related quality of life among school-aged children in regional China.

Health Qual Life Outcomes 2021 Nov 25;19(1):262. Epub 2021 Nov 25.

Nanjing Municipal Center for Disease Control and Prevention, 2, Zizhulin, Nanjing, 210003, China.

Objectives: To examine the association between health literacy (HL) and health-related quality of life (HRQoL) among primary and high school students in Nanjing, China.

Methods: A cross-sectional study was conducted among randomly selected primary (graders 4-6), junior (graders 7-9) and senior (graders 10-12) high school students in 2018 in Nanjing Municipality of China. HRQoL, the outcome variable, was assessed with the validated Chinese version of Child Health Utility 9D (CHU9D) and used as continuous variable, while HL, our independent variable, was measured with the validated Chinese Students' Health literacy Assessment Scale and treated as categorical variable ("adequate" or "inadequate") in the analysis. Mixed-effects linear regression models were introduced to calculate mean difference and 95% confidence interval (CI) for examining the association between HL and HRQoL.

Results: Totally, 4388 of 4498 students completed the survey. Among these responders, the mean score of CHU9D was 0.78 ± 0.17, and the proportion of participants with adequate HL was 85.8% (95% CI = 84.7%, 86.8%). After adjustment for potential confounders and class-level clustering effects, participants who had adequate HL were observed having, on average, an elevated HRQoL score of 0.08 (95% CI = 0.06, 0.11) units compared to their counterparts with inadequate HL. Such a positive HL-HRQoL association was also identified among each stratum of participants' age, gender and residence.

Conclusions: HL was positively associated with HRQoL score among primary and high school students in China. It has public health implications that HRQoL may be improved through school-based health literacy intervention among children and adolescents in China.
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http://dx.doi.org/10.1186/s12955-021-01895-6DOI Listing
November 2021

LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.

J Cell Biol 2022 Jan 23;221(1). Epub 2021 Nov 23.

Nanhu Laboratory, State Key Laboratory of Proteomics, National Center of Biomedical Analysis, Beijing, China.

Primary cilia transduce diverse signals in embryonic development and adult tissues. Defective ciliogenesis results in a series of human disorders collectively known as ciliopathies. The CP110-CEP97 complex removal from the mother centriole is an early critical step for ciliogenesis, but the underlying mechanism for this step remains largely obscure. Here, we reveal that the linear ubiquitin chain assembly complex (LUBAC) plays an essential role in ciliogenesis by targeting the CP110-CEP97 complex. LUBAC specifically generates linear ubiquitin chains on CP110, which is required for CP110 removal from the mother centriole in ciliogenesis. We further identify that a pre-mRNA splicing factor, PRPF8, at the distal end of the mother centriole acts as the receptor of the linear ubiquitin chains to facilitate CP110 removal at the initial stage of ciliogenesis. Thus, our study reveals a direct mechanism of regulating CP110 removal in ciliogenesis and implicates the E3 ligase LUBAC as a potential therapy target of cilia-associated diseases, including ciliopathies and cancers.
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http://dx.doi.org/10.1083/jcb.202105092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8614155PMC
January 2022

Expression of Cyclin D1 gene in ovarian cancer and effect of silencing its expression on ovarian cancer cells based on the Oncomine database.

Bioengineered 2021 Dec;12(2):9290-9300

Department of Gynecology, Sanmenxia Central Hospital of Henan University of Science and Technology, Sanmenxia, Henan Province, China.

We aimed to analyze the expression of Cyclin D1 (CCND1) gene in ovarian cancer and the influence of silencing its expression on ovarian cancer cells based on the Oncomine database. The expression of CCND1 gene in ovarian cancer was analyzed by utilizing the relevant information in different tumors and Oncomine database. The correlation between CCDN1 expression level and prognosis of ovarian cancer was analyzed by the online database Kaplan-Meier (kmplot.com). The expression of CCND1 gene in ovarian cancer and the effect of silencing its expression on cancer cells were analyzed by cell experiments. After mining and comprehensively analyzing 7 studies on the differential expression of CCND1 gene in ovarian cancer tissue and normal ovarian tissue included in the Oncomine database, it was found that the median value of CCND1 gene ranked 218.0 (P = 8.03 × 10) among all differentially expressed genes, suggesting that CCND1 gene expression in ovarian cancer tissue was higher than that in normal ovarian tissue. Adib Ovarian, Bonome Ovarian and Hendrix Ovarian microarrays revealed that the expression of CCND1 gene in ovarian cancer tissue was significantly higher than that in normal ovarian tissue (P < 0.05). Kaplan-Meier Plotter database showed that the overall survival and progression-free survival of ovarian cancer patients with high CCND1 expression were significantly shorter than those of patients with low CCND1 expression (P < 0.05). The expression levels of CCND1 gene in normal ovarian epithelial cells and SKOV3 ovarian cancer cells were detected by RT-PCR. The expression of CCND1 gene was significantly higher in SKOV3 group than that in control group (P < 0.01). Flow cytometry revealed that the percentage of cells in G/G phase was significantly higher, while that in S phase was lower in SKOV3 + siCCND1 group than the values of SKOV3 and SKOV3 + siNC groups (P < 0.05). The apoptosis rate of ovarian cancer cells was significantly higher in SKOV3 + siCCND1 group than those of SKOV3 and SKOV3 + siNC groups (P < 0.01). CCND1 gene is highly expressed in ovarian cancer tissue and related to prognosis. Preoperative evaluation of CCND1 gene expression in ovarian cancer patients may benefit the assessment of risk and prognosis.
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http://dx.doi.org/10.1080/21655979.2021.2000225DOI Listing
December 2021

Combined Effects of Resveratrol and Vitamin E From Peanut Seeds and Sprouts on Colorectal Cancer Cells.

Front Pharmacol 2021 3;12:760919. Epub 2021 Nov 3.

Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.

Resveratrol (RES) and Vitamin E (VE) are anti-cancer active ingredients with relatively high content in peanut seeds and sprouts. This study aimed to determine the synergistic inhibitory effect of RES and VE on colorectal cancer. Using 5-FU as a positive drug control, the effect of RES combined with VE on HCT-8 cells was determined, and cell viability was detected using the cell-counting kit 8 (CCK8) method. Cell morphology changes were observed using optical microscopy. Cell migration ability was evaluated by the scratch test, while cell colonies were determined by the cloning test formation ability. Apoptosis status was assessed by flow cytometry and nuclear staining by DAPI, and the expression level of apoptosis-related proteins was determined by western blotting. Compared with the single component group, the RES combined with VE group significantly inhibited the growth and proliferation of HCT-8 intestinal cancer cells . The RES combined with VE group had a greater impact on cell morphology changes and cell colony formation and significantly reduced cell migration ability and intestinal cancer cell apoptosis ( < 0.05). Additionally, combined treatment with RES and VE significantly upregulated the expression of pro-apoptotic proteins BAX, caspase-3, caspase-8, and caspase-9, and downregulated the expression of anti-apoptotic protein BCL-2, compared to the single component treatment. RES combined with VE is effective in promoting intestinal cancer cell apoptosis. This study demonstrated the significant positive synergy of RES and VE on HCT-8 cells, providing a new perspective for more effective use of RES.
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http://dx.doi.org/10.3389/fphar.2021.760919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595107PMC
November 2021

Vaccine bidding, procurement and distribution management practices in mainland China: A nationwide study.

Vaccine 2021 Nov 18. Epub 2021 Nov 18.

School of Public Health, NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, China. Electronic address:

Objective: To conduct a nationwide evaluation of vaccine bidding, procurement and distribution management for understanding and improving the current situation well in mainland China.

Methods: An institution survey was carried out to collect information on tendering, procurement, distribution and related issues to vaccines by structured questionnaires administered to 31 provinces in mainland China from April to July 2019.

Results: In 13 (41.9%) of 31 provinces, centralized bidding of National Immunization Program (NIP) vaccines was accomplished, and others conducted independent tendering and purchasing in 2018. For non-NIP vaccines, all provinces implemented unified bidding at the provincial level and over half (18, 58.1%) of them chose provincial public resource trading platforms, but their modes varied over provinces. Then procurement was undertaken by the district-level centers for disease control and prevention (CDC) while they were unable to choose the best option of too many vaccines targeted by the province-level CDC for local populations. The distribution modes of NIP and non-National Immunization Program (non-NIP) vaccines were similar overall in a province but very different over provinces. Main mode was that CDCs at different levels delivered about two thirds (64.5%) of NIP vaccines distribution and more than one third (35.5%) of non-NIP vaccines. Another distribution mode was occupied as fully buying the service from third parties in 3 municipalities. Some provinces mixed both modes, too. The total distribution volume of vaccines was 430.7 million doses in 2018. The top five non-NIP vaccines delivered in 2018 were human rabies vaccine, varicella vaccine, EV71 hand foot mouth disease vaccine, hepatitis B vaccine and influenza vaccine.

Conclusion: The vaccine biding, procurement and distribution management varied over provinces in mainland China in 2018, especially for non-NIP vaccines. Specific policies and measures should be developed for different regions to improve the immunization management better.
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http://dx.doi.org/10.1016/j.vaccine.2021.11.020DOI Listing
November 2021

Effective Rituximab Treatment in Patients with Neuromyelitis Optica Spectrum Disorders Compared with Azathioprine and Mycophenolate.

Neurol Ther 2021 Nov 19. Epub 2021 Nov 19.

Department of Neurology, The Second Medical Center and National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, No.28 Fuxing Road, Haidian District, Beijing, China.

Introduction: As an autoimmune central nervous system disease characterized by inflammation and demyelination, neuromyelitis optica (NMO) has been extensively investigated. A specific antigenic target, astrocytic water channel aquaporin-4 (AQP4) has already been identified, and it can be recognized explicitly by the autoantibody marker NMO-IgG. Along with the immune attacks, clinical disabilities would gradually accumulate. As there has been no validated and well-recognized therapy for NMO till now, preventing and postponing attack using immunosuppressive therapies is the primary treatment option.

Methods: In the current retrospective study, the effect of immunosuppressive agents was investigated through a long-term follow-up. To assess the long-term effectiveness and safety of rituximab (RTX), azathioprine (AZA), and mycophenolate mofetil (MMF) therapies, all 129 patients with NMO spectrum disorders (NMOSD) who received at least one of these treatments were studied, including 55 seropositive for AQP4-Ab and 74 seronegative for AQP4-Ab.

Results: The median post-treatment annualized relapse rate (ARR) was lower than the pre-treatment rates in all AQP4Ab groups (from 1.0 to 0.7 in RTX, from 0.8 to 0.3 in AZA, and from 0.85 to 0.35 in MMF). Meanwhile, the ARR also decreased in all AQP4Ab groups (from 0.3 to 0.2 in RTX, from 0.9 to 0.5 in AZA, and from 0.9 to 0.4 in MMF). Disability condition improved in the Expanded Disability Status Scale (EDSS) in all AQP4Ab groups (from 4.0 to 2.75 in RTX, from 3.5 to 2.5 in AZA, and from 3.0 to 2.0 in MMF) and in all AQP4Ab groups (from 3.0 to 2.5 in RTX, from 3.0 to 2.5 in AZA, and from 3.5 to 2.0 in MMF). There was no statistically significant difference between the post-treatment and pre-treatment changes of EDSS and ARR in the RTX, AZA, and MMF groups (P > 0.05). However, according to Kaplan-Meier survival analysis, RTX-treated patients were more likely to be relapse-free after long-term follow-up than those who received AZA or MMF therapy. Meanwhile, adverse effects were noted in three out of 23 patients with RTX treatment, five of 32 with AZA treatment, and three of 21 with MMF treatment. No serious adverse events were observed in all treatment groups during the study.

Conclusions: RTX, AZA, and MMF therapies efficiently lowered the relapse frequency and disability in both of the AQP4-Ab seropositive or seronegative patients with NMO. Furthermore, low dosage of RTX is recommended for the patients with NMO owing to its long-term effectiveness and safety.
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http://dx.doi.org/10.1007/s40120-021-00298-5DOI Listing
November 2021

Clinical Distribution Characteristics of 1439 Carbapenem-Resistant Strains in China: Drug Resistance, Geographical Distribution, Antibiotic MIC50/90.

Infect Drug Resist 2021 12;14:4717-4725. Epub 2021 Nov 12.

Microbiology Department, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei Province, People's Republic of China.

Purpose: To explore the clinical distribution characteristics and antimicrobial susceptibilities of carbapenem-resistant () in Hebei Province, China, from 2017 to 2019, and provide data on the treatment of this bacterial infection and the prevention of its spread.

Materials And Methods: A total of 1439 strains were collected from 2017 to 2019 in Hebei Province, China. Drug sensitivity tests were performed using the minimum inhibitory concentration (MIC) method, and the data were analyzed statistically using WHONET5.6 software.

Results: A total of 54,377 strains of were isolated in Hebei Province from 2017 to 2019, of which 1439 strains were (2.65%). The highest proportion (33.78%) of strains was isolated from urine, and the detection rate showed a slow downward trend over the past 3 years. was mainly detected in densely populated and economically developed areas. Of all the patients, 54.2% were from the medical ward; the ratio of male to female patients with infections was 1.35:1; elderly patients and adults accounted for 59.6% and 30.8%, respectively, whereas minors and newborns accounted for 4.9% and 4.7%, respectively. For , the drug resistance rates to β-lactams were all higher than 80% and there was an annual increasing trend, while the drug resistance rates to quinolones remained nearly unchanged. The rate of resistance to aminoglycosides was relatively low, especially to amikacin (approximately 22%). The MIC50 of other antibacterial drugs, except amikacin, was equal to or higher than the break point of drug resistance.

Conclusion: From 2017 to 2019, the isolation rate of in Hebei Province, China, remained stable; however, the drug resistance rate showed an upward trend, primarily in cases of urinary tract infections in older men; the resistance rate to amikacin was the lowest.
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http://dx.doi.org/10.2147/IDR.S334283DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8594617PMC
November 2021

Role of Outer Membrane Vesicles From in Atherosclerosis.

Front Cell Dev Biol 2021 1;9:673993. Epub 2021 Nov 1.

Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, China.

Infection is thought to be involved in the pathogenesis of atherosclerosis. Studies have shown the association between () and coronary artery disease. It is interesting to find DNA and cytotoxin-associated gene A (CagA) protein in atherosclerotic plaque. Outer membrane vesicles (OMVs), secreted by , exert effects in the distant organ or tissue. However, whether or not OMVs from are involved in the pathogenesis of atherosclerosis remains unknown. Our present study found that treatment with OMVs from CagA-positive accelerated atherosclerosis plaque formation in ApoE mice. -derived OMVs inhibited proliferation and promoted apoptosis of human umbilical vein endothelial cells (HUVECs), which was also reflected in studies. These effects were normalized to some degree after treatment with lipopolysaccharide (LPS)-depleted CagA-positive OMVs or CagA-negative OMVs. Treatment with -derived OMVs increased reactive oxygen species (ROS) levels and enhanced the activation of nuclear factor-κB (NF-κB) in HUVECs, which were reversed to some degree in the presence of a superoxide dismutase mimetic TEMPOL and a NF-κB inhibitor BAY11-7082. Expressions of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α), two inflammatory factors, were augmented after treatment with OMVs from These suggest that -derived OMVs accelerate atherosclerosis plaque formation via endothelium injury. CagA and LPS from -OMVs, at least in part, participate in these processes, which may be involved with the activation of ROS/NF-κB signaling pathway. These may provide a novel strategy to reduce the incidence and development of atherosclerosis.
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http://dx.doi.org/10.3389/fcell.2021.673993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591407PMC
November 2021

Reference Interval for Pulse Oxygen Saturation in Neonates at Different Altitudes: A Systematic Review.

Front Pediatr 2021 1;9:771750. Epub 2021 Nov 1.

Department of Neonatology, Qinghai University Affiliated Hospital, Xining, China.

The reference interval for pulse oxygen saturation (SpO) in neonates born at high altitudes has not been defined to date. The purpose of this study was to systematically review published studies and determine the reference interval of SpO in neonates at different altitudes. Databases of PubMed, Embase, Cochrane Library, Clinicaltrials.Gov, Chinese National Knowledge Infrastructure Database, Wanfang Database, Chinese Science Technology Journals Database, and Chinese Clinical Trial Registry were searched for studies reporting SpO in healthy neonates at different altitudes. Retrieval time was from inception of the database to August 16, 2021. The Agency for Healthcare Research and Quality checklist was used to evaluate the quality of studies. Python v3.8 was used to analyze the data. This systematic review was drafted in accordance with the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Seven cross-sectional studies, published between 1991 and 2020, were identified. They were from US, Mexico, Israel, Ecuador, and China. Three studies were rated as high quality and four as moderate quality. The mean SpO (with standard deviation or standard error) of neonates born in 40 different altitudes (ranging from 25 meters to 3,100 meters) were obtained. The prediction equation for calculation of the lower limit of the reference interval was established, and the reference intervals for SpO at different altitudes were determined. In healthy neonates, the lower limit of the reference interval of SpO decreases with increase in altitude. High-quality prospective studies are need to confirm our findings.
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http://dx.doi.org/10.3389/fped.2021.771750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8591307PMC
November 2021

Loss of MBD2 attenuates MLL-AF9-driven leukemogenesis by suppressing the leukemic cell cycle via CDKN1C.

Oncogenesis 2021 Nov 17;10(11):79. Epub 2021 Nov 17.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Acute myeloid leukemia (AML) is a deadly cancer characterized by an expanded self-renewal capacity that is associated with the accumulation of immature myeloid cells. Emerging evidence shows that methyl-CpG-binding domain protein 2 (MBD2), a DNA methylation reader, often participates in the transcriptional silencing of hypermethylated genes in cancer cells. Nevertheless, the role of MBD2 in AML remains unclear. Herein, by using an MLL-AF9 murine model and a human AML cell line, we observed that loss of MBD2 could delay the initiation and progression of leukemia. MBD2 depletion significantly reduced the leukemia burden by decreasing the proportion of leukemic stem cells (LSCs) and inhibiting leukemia cell proliferation in serial transplantation experiments, thereby allowing leukemic blasts to transition to a more mature state reflecting normal myelopoiesis. Both gene expression analyses and bioinformatic studies revealed that MBD2 negatively modulated genes related to myeloid differentiation, and was necessary to sustain the MLL-AF9 oncogene-induced gene program. We further demonstrated that MBD2 could promote LSC cell cycle progression through epigenetic regulation of CDKN1C transcription probably by binding to its promoter region. Taken together, our data suggest that MBD2 promotes AML development and could be a therapeutic target for myeloid malignancies.
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http://dx.doi.org/10.1038/s41389-021-00366-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599466PMC
November 2021

Pb contaminated soil influence on aboveground biomass and bioactive compounds in leaves of mulberry.

Int J Phytoremediation 2021 Nov 17:1-7. Epub 2021 Nov 17.

College of Biotechnology, Jiangsu University of Science and Technology, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, China.

This study investigated the effects of soil lead (Pb), biochar, and partial root zone drought (PRD) on mulberry ( L.) branches and leaves biomass, Pb accumulation, and bioactive compounds including 1-deoxynojirimycin (DNJ) and flavonoids. Three-factor pot experiments were conducted with biochar, PRD, and soil Pb at four concentration levels (0, 50, 200, and 800 mg kg). Results revealed that mulberry aboveground biomass did not decrease significantly across the soil Pb levels. Pb concentration of mulberry leaves do not increase significantly when soil Pb was 200 mg kg; however, it significantly accumulated under 800 mg kg. There was a dose-effect between the Pb concentration in branches and the soil Pb levels. Mulberry leaf flavonoids were affected by the interaction of soil Pb and biochar. The interaction between two of the three factors significantly affected leaves DNJ concentration. The combination of biochar and PRD maintained the biomass of mulberry and did not significantly increase Pb in leaves under 200 mg kg soil Pb concentration. In summary, mulberry has a higher resistance to soil Pb stress, and it can be planted in moderate Pb-contaminated soils for no loss of biomass and can safely harvest the branches and leaves.Novelty statementAn economic benefit is a key to the practical application and sustainability of phytoremediation. Based on this, we studied the effects of soil Pb on biomass, Pb accumulation, and bioactive substance concentration of harvesting organs in mulberry.Phytoremediation is not isolated, and techniques, such as soil amendments and water management also play a role. In this study, we found that biochar and partial root-zone irrigation had a synergistic effect on the response of mulberry to soil Pb, which could be co-applied in the phytoremediation of lead-contaminated soil.The concentration of heavy metals is the key to ensuring product safety in heavy metal contaminated soil. We found that Pb concentration in leaf and stem of mulberry did not significantly increase under 200 mg kg soil Pb, while increased at 800 mg kg soil Pb. Therefore, planting mulberry on 200 mg kg Pb contaminated soil can safely harvest branches and leaves.
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http://dx.doi.org/10.1080/15226514.2021.2002808DOI Listing
November 2021

Stapled trans-anal rectal resection can improve constipation symptoms and inflammatory reaction of patients with outlet obstructive constipation.

Authors:
Tao Lian Na Wang

Am J Transl Res 2021 15;13(10):11472-11481. Epub 2021 Oct 15.

Six Disease Area of Colorectal and Anal Surgery, Shanxi Provincial People's Hospital Taiyuan 030012, Shanxi Province, China.

Objective: To explore the effect of stapled trans-anal rectal resection (STARR) on constipation symptoms and inflammatory reaction in patients with outlet obstructive constipation (OOC).

Methods: From January 2019 to June 2020, a retrospective analysis was conducted on the medical data of 124 patients with OOC admitted to our hospital. According to the different surgical methods, sixty patients were assigned to the control group (CG) treated with Bresler operation, and sixty-four patients were included in the research group (RG) receiving STARR. The total effective rate of treatment, the incidence of postoperative complications, and mid- and long-term recurrence rate were observed between the two groups. The anorectal dynamic indexes, the constipation scoring system (CSS), obstructive defecation syndrome (ODS), visual analogue scale (VAS) scores, and the changes of inflammatory cytokine [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] were compared between the two groups, and the perioperative indexes were observed.

Results: After operation, a remarkably higher total effective rate was observed in the RG as compared to the CG. The RG obtained lower results in terms of the incidence of postoperative complications, 3-year recurrence rate, operation cost, and intraoperative blood loss than CG. Compared with patients in the CG, those in the RG had shorter operation time, first exhaust time, time of leaving bed, and hospitalization time. Moreover, a shorter defecation time of patients in the RG was also obtained 14 days after treatment. In addition to rectal rest pressure that was similar between the two groups, the anal maximal contraction pressure, anal longest contraction time, anorectal relaxation pressure, and anal rest pressure were significantly higher in the RG than in the CG. After operation, remarkably lower CSS and ODS scores of patients were obtained in the RG than in the CG. The RG yielded lower VAS scores after operation and 24 hours after operation, and lower levels of TNF-α and IL-6 1 day after operation in contrast to the CG.

Conclusion: STARR can effectively improve the clinical efficacy, ameliorate the symptoms of postoperative constipation, reduce the long-term recurrence rate, relieve postoperative pain, and better protect the anorectal function and mitigate inflammatory reaction for patients with obstructive constipation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8581840PMC
October 2021

Collagen-based biocomposites inspired by bone hierarchical structures for advanced bone regeneration: ongoing research and perspectives.

Biomater Sci 2021 Nov 16. Epub 2021 Nov 16.

College of Marine Life Science, Ocean University of China, Qingdao, 266003, P.R. China.

Bone is a hard-connective tissue composed of matrix, cells and bioactive factors with a hierarchical structure, where the matrix is mainly composed of type I collagen and hydroxyapatite. Collagen fibers assembled by collagen are the template for mineralization and make an important contribution to bone formation and the bone remodeling process. Therefore, collagen has been widely clinically used for bone/cartilage defect regeneration. However, pure collagen implants, such as collagen scaffolds or sponges, have limitations in the bone/cartilage regeneration process due to their poor mechanical properties and osteoinductivity. Different forms of collagen-based composites prepared by incorporating natural/artificial polymers or bioactive inorganic substances are characterized by their interconnected porous structure and promoting cell adhesion, while they improve the mechanical strength, structural stability and osteogenic activities of the collagen matrix. In this review, various forms of collagen-based biocomposites, such as scaffolds, sponges, microspheres/nanoparticles, films and microfibers/nanofibers prepared by natural/synthetic polymers, bioactive ceramics and carbon-based materials compounded with collagen are reviewed. In addition, the application of collagen-based biocomposites as cytokine, cell or drug (genes, proteins, peptides and chemosynthetic) delivery platforms for proangiogenesis and bone/cartilage tissue regeneration is also discussed. Finally, the potential application, research and development direction of collagen-based biocomposites in future bone/cartilage tissue regeneration are discussed.
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http://dx.doi.org/10.1039/d1bm01294kDOI Listing
November 2021

Circular RNA intraflagellar transport 80 facilitates endometrial cancer progression through modulating miR-545-3p/FAM98A signaling.

J Gynecol Oncol 2021 Oct 25. Epub 2021 Oct 25.

Department of Gynecological Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.

Objective: Endometrial cancer (ECa) is a common gynecological malignancy. Circular RNAs (circRNAs) have been identified as key regulators of human tumorigenesis and development. Herein, we explored the role and mechanism of circular RNA intraflagellar transport 80 (circ-IFT80, also called circ_0067835) in ECa.

Methods: Circ-IFT80, microRNA-545-3p (miR-545-3p), and family with sequence similarity 98 member A (FAM98A) were quantified by quantitative real-time polymerase chain reaction or Western blot. The biological characteristics of ECa cells were evaluated via Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine, transwell, tube formation and flow cytometry assays. Dual-luciferase reporter assay or RNA pull-down assay was employed to verify the binding relationship between miR-545-3p and circ-IFT80 or FAM98A. Xenograft assays were conducted to analyze the effect of circ-IFT80 in vivo.

Results: Circ-IFT80 and FAM98A were up-regulated, and miR-545-3p was down-regulated in ECa tissues and cells. Knockdown of circ-IFT80 blocked proliferation, migration, invasion and angiogenesis and promoted apoptosis in ECa cells. Moreover, circ-IFT80 harbored a binding site for miR-545-3p, and the effects of circ-IFT80 were mediated by miR-545-3p. FAM98A was a direct target of miR-545-3p, and miR-545-3p hindered ECa cell progression via targeting FAM98A. Circ-IFT80 induced FAM98A expression through miR-545-3p. Furthermore, silence of circ-IFT80 suppressed tumor growth in vivo.

Conclusion: Circ-IFT80 may promote the malignant progression of ECa cells at least in part by modulating miR-545-3p/FAM98A axis, providing a potential therapeutic target for ECa.
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http://dx.doi.org/10.3802/jgo.2022.33.e2DOI Listing
October 2021

A Recurring Chemogenetic Switch for Chimeric Antigen Receptor T Cells.

Angew Chem Int Ed Engl 2021 Nov 15. Epub 2021 Nov 15.

Texas A&M University, Chemistry, UNITED STATES.

As a revolutionary cancer treatment, the chimeric antigen receptor (CAR) T cell therapy suffers from complications such as cytokine release syndromes and T cell exhaustion. Their mitigation desires controllable activation of CAR-T cells that is achievable through regulatory display of CARs. By embedding the hepatitis C virus NS3 protease (HCV-NS3) between the single-chain variable fragment (scFv) and the hinge domain, we showed that the display of anti-CD19 scFv on CAR-T cells was positively correlated to the presence of a clinical HCV-NS3 inhibitor asunaprevir (ASV). This novel CAR design that allows the display of anti-CD19 scFv in the presence of ASV and its removal in the absence of ASV creates a practically recurring chemical switch. We demonstrated that the intact CAR on T cells can be repeatedly turned on and off by controlling the presence of ASV in a dose dependent manner both in vitro and in vivo, which enables delicate modulation of CAR-T activation during cancer treatment.
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http://dx.doi.org/10.1002/anie.202109550DOI Listing
November 2021

Study of the joint action of multi-component mixtures based on parameter σ characterizing the shape difference of concentration-response curves.

Environ Pollut 2021 Nov 12;293:118486. Epub 2021 Nov 12.

Shandong Dongyuan New Material Technology Co., Ltd., Dongying, 257300, Shandong, China.

A previous study has revealed that the parameter k∙EC, characterizing the shape of concentration-response curves (CRCs), could predict the combined toxicity of binary mixtures. This study further explored the predictability of multi-component mixtures. Eleven component mixtures were designed using the uniform design ray, and the acute toxicity of the eleven environmental pollutants and their mixtures to Vibrio fischeri was determined using microplate toxicity analysis. We used independent action (IA) and the effect residual ratio (ERR) models to evaluate the combined toxicity of multi-component mixtures and ascertain the functional relationship between σ, a parameter characterizing the CRC morphological difference of multi-component mixtures, and combined toxicity. The variance σ of each component characteristic parameter of multi-component mixtures gradually increased in the concentration range, and the relationship between σ and ERR was consistent with the exponential function. The literature verification showed that this rule is generally applicable to the acute toxicity of multi-component mixtures to luminescent bacteria. The exponential function showed the variation rule of the joint action of multi-component mixtures. In the present study, the joint toxicity of multi-component mixtures can be predicted from single toxicity and small amount of multiple toxicity, circumventing complex multi-component toxicity experiments.
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http://dx.doi.org/10.1016/j.envpol.2021.118486DOI Listing
November 2021

The stress granule protein G3BP1 promotes pre-condensation of cGAS to allow rapid responses to DNA.

EMBO Rep 2021 Nov 15:e53166. Epub 2021 Nov 15.

State Key Laboratory of Proteomics, National Center of Biomedical Analysis, Beijing, China.

Cyclic GMP-AMP synthase (cGAS) functions as a key sensor for microbial invasion and cellular damage by detecting emerging cytosolic DNA. Here, we report that GTPase-activating protein-(SH3 domain)-binding protein 1 (G3BP1) primes cGAS for its prompt activation by engaging cGAS in a primary liquid-phase condensation state. Using high-resolution microscopy, we show that in resting cells, cGAS exhibits particle-like morphological characteristics, which are markedly weakened when G3BP1 is deleted. Upon DNA challenge, the pre-condensed cGAS undergoes liquid-liquid phase separation (LLPS) more efficiently. Importantly, G3BP1 deficiency or its inhibition dramatically diminishes DNA-induced LLPS and the subsequent activation of cGAS. Interestingly, RNA, previously reported to form condensates with cGAS, does not activate cGAS. Accordingly, we find that DNA - but not RNA - treatment leads to the dissociation of G3BP1 from cGAS. Taken together, our study shows that the primary condensation state of cGAS is critical for its rapid response to DNA.
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http://dx.doi.org/10.15252/embr.202153166DOI Listing
November 2021

The Role of miR-23b in Cancer and Autoimmune Disease.

J Oncol 2021 3;2021:6473038. Epub 2021 Nov 3.

National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry (Shaanxi Normal University), The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, China.

Short-stranded miRNAs are single-stranded RNA molecules involved in the regulation of gene expression. miRNAs are involved in a variety of cellular physiological processes, including cell proliferation, differentiation, and apoptosis. miR-23b have been identified to act both as oncogenes and as tumor suppressors. In addition, miR-23b is related to inflammation resistance to various autoimmune diseases and restrained inflammatory cell migration. The characterization of the specific alterations in the patterns of miR-23b expression in cancer and autoimmune disease has great potential for identifying biomarkers for early disease diagnosis, as well as for potential therapeutic intervention in various diseases. In this review, we summarize the ever-expanding role of miR-23b and its target genes in different models and offer insight into how this multifunctional miRNA modulates tumor cell proliferation and apoptosis or inflammatory cell activation, differentiation, and migration.
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http://dx.doi.org/10.1155/2021/6473038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8580694PMC
November 2021

Adiposity and Long-Term Adiposity Change Are Associated with Incident Diabetes: A Prospective Cohort Study in Southwest China.

Int J Environ Res Public Health 2021 10 31;18(21). Epub 2021 Oct 31.

School of Public Health, Key Laboratory of Public Health Safety, NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai 200032, China.

In order to estimate the associations of different adiposity indicators and long-term adiposity changes with risk of incident type 2 diabetes (T2DM), we conducted a 10-year prospective cohort study of 7441 adults in Guizhou, China, from 2010 to 2020. Adiposity was measured at baseline and follow-up. Cox proportional hazard models were used to estimated hazard ratios (HRs) and 95% confidence intervals (95% CIs). A total of 764 new diabetes cases were identified over an average follow-up of 7.06 years. Adiposity indicators, body mass index (BMI), waist circumference (WC), waist-height ratio (WHtR), and long-term adiposity changes (both weight change and WC change) were significantly associated with an increased risk of T2DM (adjusted HRs: 1.16-1.48). Significant non-linear relationships were found between weight/WC change and incident T2DM. Compared with subjects with stable WC from baseline to follow-up visit, the subjects with WC gain ≥9 cm had a 1.61-fold greater risk of T2DM; those with WC loss had a 30% lower risk. Furthermore, the associations were stronger among participants aged 40 years or older, women, and Han Chinese. Preventing weight or WC gain and promoting maintenance of normal body weight or WC are important approaches for diabetes prevention, especially for the elderly, women, and Han Chinese.
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http://dx.doi.org/10.3390/ijerph182111481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8582792PMC
October 2021

The comparison of different drip cholangiography: a randomised trial.

Ann Palliat Med 2021 10;10(10):10797-10803

Department of Hand, Foot and Ankle Surgery, General Hospital of Ningxia Medical University, Yinchuan, China.

Background: This study investigated the advantages and disadvantages of contrast media administration by gravity drip and manual push injection during cholangiography.

Methods: A total of 100 patients who presented to the Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, for a cholangiography between June 2019 to June 2020 were enrolled in this study. Patients were randomly divided into 2 treatment groups. One group of patients with manual injection of contrast (the N group, n=50), received the contrast agent via the traditional manual injection method whereby the doctor injects 50 mL of prepared contrast agent into the right side of the patient while continuously observing the effects on the bile duct. The other group of patients with gravity drip administration of contrast media (the O group, n=50), received the contrast agent via gravity drip at a rate of 80 drops per minute, and both clinicians and radiologists monitored the entire cholangiography process from a safe distance. Patients were followed up and angiographic satisfaction was assessed after two weeks.

Results: All 100 patients completed cholangiography without allergic reaction to the contrast medium. In the traditional injection group (N group), nine patients experienced upper abdominal discomfort with nausea, abdominal pain, chills, high fever, and other symptoms, and residual gallstones were observed in 12 patients. In patients in the gravity drip group (O group), four patients felt upper abdominal discomfort accompanied by nausea, abdominal pain, chills, high fever, and other symptoms, with residual gallstones detected in six patients.

Conclusions: Patients who underwent gravity drip cholangiography had significantly reduced adverse reactions compared to patients who underwent traditional manual infusion cholangiography. Furthermore, gravity drip cholangiography resulted in clearer images and reduced X-ray exposure for medical staff. Thus, increased implementation of gravity drip cholangiography in the clinical setting should be considered.

Trial Registration: Chinese Clinical Trial Registry ChiCTR1800018202.
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http://dx.doi.org/10.21037/apm-21-2661DOI Listing
October 2021

Analysis of Psychological Trends and Policy Recommendations of Medical Staff in Northern China in the Latter Stages of the COVID-19 Pandemic.

Front Psychol 2021 20;12:747557. Epub 2021 Oct 20.

Department of Psychology, The People's Hospital of Liaoning Province, The People's Hospital of China Medical University, Shenyang, China.

Since the 2019 coronavirus disease (COVID-19) outbreak, medical staff have faced greater psychological stress and are prone to psychological problems such as anxiety and depression, as confirmed by several studies. This study further clarifies the psychological status of Chinese medical staff during the stable phase of the pandemic through a cross-sectional investigation in a large population sample in northern China. Subjects: Clinical frontline medical staff from seven hospitals in Liaoning Province were recruited from November 2020 to February 2021. The research tools used were the Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS), Simplified Coping Style Questionnaire (SCSQ), and General Status Questionnaire. SPSS 22.0, ANOVA variance analysis, and multiple logistics regression were used for statistical analysis. -values of <0.05 indicated significant statistical differences. A total of 3,144 medical staff completed the survey (599 men [19.1%] and 2,545 women [80.9%]; 1,020 doctors [32.4%] and 2,124 nurses [67.6%]). Among all subjects, the rates of anxiety and depression were 21.1% (663/3, 144) and 43.9% (1,381/3,144), respectively. Multiple logistic comparative analysis revealed that age (OR = 1.272, 95% CI = 1.036-1.561, = 0.022), the need for psychological counseling (OR = 1.566, 95% CI = 1.339-1.830, < 0.001), and the coexistence of depression (OR = 0.050, 95% CI = 0.038-0.066, < 0.001) were significantly associated with anxiety. Coexisting anxiety was also associated with the occurrence of depression (OR = 0.050, 95% CI = 0.038-0.065, < 0.001). In the later stages of the pandemic in China, the occurrence rates of anxiety and depression among medical staff remain high. In addition to age, there is little correlation between anxiety or depression and general factors such as gender and profession. As a special group, medical staff show different psychological changes at various times during a stressful event. Concerning for the psychological needs of medical staff and different psychologically oriented policy implementation are needed.
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http://dx.doi.org/10.3389/fpsyg.2021.747557DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564395PMC
October 2021

Prevalences of metabolic syndrome and its sex-specific association with socioeconomic status in rural China: a cross-sectional study.

BMC Public Health 2021 Nov 6;21(1):2033. Epub 2021 Nov 6.

School of Public Health; Key Laboratory of Public Health Safety, NHC Key Laboratory of Health Technology Assessment, Fudan University, Shanghai, 200032, China.

Background: Few studies have reported the prevalence of metabolic syndrome (MS) and examined relationships between socioeconomic status and MS in rural China. This study aimed to evaluate the prevalence of MS and MS components as well as their associations with socioeconomic status among rural Chinese adults.

Methods: A cross-sectional study of 26,836 participants aged 20 years and older was conducted from June to December 2012 in Yuhuan City, Zhejiang Province, China, which is located on Yuhuan Island. A multivariable logistic regression model was used to identify risk factors for MS and their possible interactions.

Results: Among 26,836 subjects with an average age of 53.4 ± 14.0 years, 59% were female. The overall prevalence of MS was 20.5%, and there was a significant sex difference in the prevalence (15.1% for males vs. 24.2% for females, P < 0.001). Compared with males, females also showed a significantly higher proportion of most MS components. A significantly higher prevalence of MS was found among subjects who were elderly, had a lower income level, had a lower level of education, or were unemployed. Multiple significant interactions were observed between the prevalence of MS and sex, age or socioeconomic status (P < 0.001). The risk of MS increased significantly with age in females but not in males. Additionally, a lower income level and a lower level of education were significantly related to an increased risk only in females, and unemployed males had a higher risk of MS than unemployed females.

Conclusions: The prevalence of MS and its components was relatively high in a rural island Chinese population with rapid urbanization, and sex-specific associations between socioeconomic factors and MS were found. Targeted preventive interventions should be developed and implemented to prevent and control MS among those with low socioeconomic status, especially females.
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http://dx.doi.org/10.1186/s12889-021-12074-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8572445PMC
November 2021

Inhibition of SREBP-mediated lipid biosynthesis and activation of multiple anticancer mechanisms by platinum complexes: Ascribe possibilities of new antitumor strategies.

Eur J Med Chem 2021 Oct 14;227:113920. Epub 2021 Oct 14.

Inner Mongolia University Research Center for Glycochemistry of Characteristic Medicinal Resources, Department of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021, People's Republic of China. Electronic address:

Cancer is one of the most aggressive diseases with poor prognosis and survival rates. Lipids biogenesis play key role in cancer progression, metastasis and tumor development. Suppression of SREBP-mediated lipid biogenesis pathway has been linked with cancer inhibition. Platinum complexes bearing good anticancer effect and multiple genes activation properties are considered important and increase the chances for development of new platinum-based drugs. In this study, we synthesized pyridine co-ligand functionalized cationic complexes and characterized them using multiple spectroscopic and spectrophotometric methods. Two of these complexes were studied in solid state by single crystal X-ray analysis. The stability of these complexes were measured in solution state using H NMR methods. These complexes were further investigated for their anticancer activity against human breast, lung and liver cancer cells. MTT assay showed potential cytotoxic activity in dose-dependent manner and decrease survival rates of cancer cells was observed upon treatment with these complexes. Biological assays results revealed higher cytotoxicity as compared to cisplatin and oxaliplatin. Further we studied C2, C6 and C8 in detailed mechanistic anticancer analyses. Clonogenic assay showed decrease survival of MCF-7, HepG2 and A549 cancer cells treated with C2, C6 and C8 as compared to control cells treated with DMSO. TUNEL assay showed more cell death, these complexes suppressed invasion and migration ability of cancer cells and decreased tumor spheroids formation, thus suggesting a potential role in inhibition of cancer metastasis and cancer stem cells formation. Mechanistically, these complexes inhibited sterol regulatory element-binding protein 1 (SREBP-1) expression in cancer cells in dose-dependent manner and thereby reduced lipid biogenesis to suppress cancer progression. Furthermore, expression level was decreased for the key genes LDLR, FASN and HMGCR, those required for sterol biosynthesis. Taken together, these complexes suppressed cancer cell growth, migration, invasion and spheroids formation by inhibiting SREBP-1 mediated lipid biogenesis pathway.
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http://dx.doi.org/10.1016/j.ejmech.2021.113920DOI Listing
October 2021

TCF-1 PD-1 CD8T cells are associated with the response to PD-1 blockade in non-small cell lung cancer patients.

J Cancer Res Clin Oncol 2021 Nov 2. Epub 2021 Nov 2.

Department of Respiratory and Critical Care Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Purpose: To determine whether TCF-1PD-1CD8T cells are associated with the response to PD-1 blockade in non-small cell lung cancer (NSCLC) patients.

Methods: We investigated the expression of TCF-1PD-1CD8T cells and elucidated their predictive role in NSCLC patients. Pretreatment specimens from 20 advanced NSCLC patients who underwent PD-1 immunotherapy or combined with chemotherapy were analyzed. The frequencies of TCF-1 cells in PD-1CD8T cells were determined in these biospecimens using multi-label immunofluorescence staining and multi-spectral acquisition technology. The clinical roles of TCF-1PD-1CD8T cells were assessed via analyzing our cases and human NSCLC data collected from public databases.

Results: A high frequency of TCF-1PD-1CD8T cells was identified in responders compared with non-responders (p = 0.0024), and the patients with high expression of this cell subset had durable clinical benefit of anti-PD-1 therapy. There were no significant association between the expression of TCF-1PD-1CD8T cells and patients' age, smoking history, pathologic type, and genetic status. In univariate analysis by the Cox hazard model, high frequency of TCF-1 PD-1 CD8T cells was significantly correlated with patients' benefit of PD-1 blockade (p = 0.024).

Conclusion: Our study indicated that TCF-1PD-1CD8T cells are associated with the response to PD-1 blockade, and may be a predictor of anti-PD-1 therapy.
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http://dx.doi.org/10.1007/s00432-021-03845-7DOI Listing
November 2021

Atomically Dispersed Ruthenium on Nickel Hydroxide Ultrathin Nanoribbons for Highly Efficient Hydrogen Evolution Reaction in Alkaline Media.

Adv Mater 2021 Nov 18;33(44):e2104764. Epub 2021 Sep 18.

Department of Physical Chemistry, School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, No. 30, Xueyuan Road, Haidian District, Beijing, 100083, China.

Achieving highly efficient hydrogen evolution reaction (HER) in alkaline media is a great challenge. Single-atom catalysts with high-loading amount have attracted great interest due to their remarkable catalytic properties. Herein, by using nickel foam as the substrate, the authors design and precisely synthesize atomic ruthenium (Ru)-loaded nickel hydroxide ultrathin nanoribbons (R-NiRu) with a high atomic Ru loading amount reaching ≈7.7 wt% via a one-step hydrothermal method. The presence of concentrated Cl in the synthetic system is beneficial for constructing ultrathin nanoribbons, which, with abundant edge OH groups, make it easy to trap atomic Ru. Taking advantage of the synergy between atomic Ru and the nanoribbon morphology of nickel hydroxide, R-NiRu exhibit a low overpotential of 16 mV for HER at 10 mA cm and a Tafel slope of 40 mV dec in aqueous 1.0 m KOH solution, which are superior to those of commercial Pt/C (overpotential of 17 mV at 10 mA cm , Tafel slope of 43 mV dec ). Density functional theory (DFT) calculation results demonstrate that atomically dispersed Ru can significantly reduce the HER energy barrier. Moreover, R-NiRu maintains exceptional stability after 5000 cyclic voltammetry cycles. This efficient and facile synthetic strategy provides a new avenue for designing efficient catalysts.
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http://dx.doi.org/10.1002/adma.202104764DOI Listing
November 2021

Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for the Analysis of Complex Compounds in Serum and Its Application in Accurate Detection of Early Arsenic Exposure.

ACS Omega 2021 Oct 12;6(42):28326-28333. Epub 2021 Oct 12.

National Center for Biomedical Analysis, 27 Tai-Ping Road, Beijing 100850, China.

With the acceleration of industrialization, environmental arsenic pollution is threatening human health. However, by the time clinical symptoms appear, arsenic toxicity has usually caused irreversible damage to the body, so it is important to establish a rapid and accurate screening method for early arsenic exposure. In this work, 32 female C57BL/6 mice were exposed to different concentrations of inorganic arsenic in drinking water for a week. By analyzing the changes in serum, more than 20 compounds were detected to increase or decrease with the increase of arsenic intake. The abnormal increase in inosine, xanthine, xanthosine, and hypoxanthine and the abnormal purine pathway were found at the same time. Dimethylarsenic acid, an important inorganic arsenic metabolite in the body, was also found in serum. Combined with statistical analysis, early arsenic exposure can be easily and quickly detected, and the potential health risks of short-term exposure can be revealed simultaneously.
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http://dx.doi.org/10.1021/acsomega.1c04517DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8552457PMC
October 2021

Effective inhibition of coronavirus replication by .

Front Biosci (Landmark Ed) 2021 10;26(10):789-798

Department of Pharmacognosy, Pharmaceutical College, Hebei Medical University, 050017 Shijiazhuang, Hebei, China.

: The coronavirus disease 2019 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected more than 210 million individuals globally and resulted in over 4 million deaths since the first report in December 2019. The early use of traditional Chinese medicine (TCM) for light and ordinary patients, can rapidly improve symptoms, shorten hospitalization days and reduce severe cases transformed from light and normal. Many TCM formulas and products have a wide application in treating infectious and non-infectious diseases. Sieb. et Zucc. (), is an important Traditional Chinese Medicine with actions of clearing away heat and eliminating dampness, draining the gallbladder to relieve jaundice, removing blood stasis to alleviate pain, resolving phlegm and arrest cough. In the search for anti-SARS-CoV-2, was recommended as as a therapeutic drug of COVID-19 pneumonia.In this study, we aimed to identifies is the potential broad-spectrum inhibitor for the treatment of coronaviruses infections. : In the present study , we infected human malignant embryonal rhabdomyoma (RD) cells with the OC43 strain of the coronavirus, which represent an alternative model for SARS-CoV-2 and then employed the cell viability assay kit for the antiviral activity. We combined computer aided virtual screening to predicte the binding site and employed Surface plasmon resonance analysis (SPR) to comfirm the interaction between drugs and coronavirus. We employed fluorescence resonance energy transfer technology to identify drug's inhibition in the proteolytic activity of 3CLpro and Plpro. : Based on our results, polydatin and resveratrol derived from P. cuspidatum significantly suppressed HCoV-OC43 replication. 50% inhibitory concentration (IC) values of polydatin inhibited SARS-CoV-2 Mpro and Plpro, MERS Mpro and Plpro were 18.66, 125, 14.6 and 25.42 μm, respectively. IC values of resveratrol inhibited SARS-CoV-2 Mpro and Plpro, MERS Mpro and Plpro were 29.81 ,60.86, 16.35 and19.04 μM, respectively. Finally, SPR assay confirmed that polydatin and resveratrol had high affinity to SARS-CoV-2, SARS-CoV 3Clpro, MERS-CoV 3Clpro and PLpro protein. : we identified the antiviral activity of flavonoids polydatin and resveratrol on RD cells. Polydatin and resveratrol were found to be specific and selective inhibitors for SARS-CoV-2, 3CLpro and PLpro, viral cysteine proteases. In summary, this study identifies as the potential broad-spectrum inhibitor for the treatment of coronaviruses infections.
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http://dx.doi.org/10.52586/4988DOI Listing
October 2021
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