Publications by authors named "Na Liu"

2,191 Publications

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Screening of the heterotrophic microalgae strain for the reclamation of acid producing wastewater.

Chemosphere 2022 Aug 14:136047. Epub 2022 Aug 14.

School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China.

For the sustainable development of the environment, to reduce the high cost and low productivity of microalgae biofuel, nine microalgae strains were screened to study the growh and nutrient removal properties under heterotrophic culture by using the waste carbon source of volatile fatty acids (VFAs). Chlorella sorokiniana (C.sorokiniana) was selected as the best strain with the highest biomass concentration of 0.77 g L, specific growth rate of 0.25 d, biomass productivity of 91.43 mg L d, total nitrogen removal efficiency of 95.96% and total phosphorus removal efficiency of 93.42%. To study the utilization potential of acid-producing wastewater by heterotrophic microalgae, actual acid-producing wastewater was recycled three times for the utilization of C.sorokiniana. After the three utilization cultivation, the removal rates of COD, total nitrogen, ammonia nitrogen, and total phosphorus were 74.44%, 88.05%, 79.08%, and 82.69%, respectively. The total utilization rates of acetic acid, propionic acid, and butyric acid were 58.99%, 70.54%, and 81.52%, respectively. In addition, the highest lipid content of 39.15% and protein content of 42.43% achieved at the third cultivation. After the first cultivation, the composition and diversity of the microbial community structure changed dramatically, with Protebacteria, Bacteroidota, Hydrogenophaga, and Algoriphagus becoming enriched. These results showed a promising way of coupling wastewater treatment with biomass production for long-term sustainability of microalgae lipid production.
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http://dx.doi.org/10.1016/j.chemosphere.2022.136047DOI Listing
August 2022

Meta-Analysis of Pulmonary Artery Denervation for Treatment of Pulmonary Hypertension.

Braz J Cardiovasc Surg 2022 Aug 16;37(4):554-565. Epub 2022 Aug 16.

Department of Cardiovascular Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, People's Republic of China.

Introduction: Pulmonary artery denervation (PADN) can reduce the sympathetic nervous system (SNS) activity, reduce pulmonary artery pressure (PAP), and improve the quality of life in patients with pulmonary hypertension (PH). We conducted a systematic meta-analysis of the effectiveness of PADN in the treatment of PH patients.

Methods: This is a comprehensive literature search including all public clinical trials investigating the effects of PADN on PH. Outcomes were mean pulmonary artery pressure (mPAP), pulmonary vascular resistance (PVR), cardiac output (CO), right ventricular (RV) Tei index, 6-minute walk distance (6MWD), and New York Heart Association (NYHA) cardiac function grading.

Results: A total of eight clinical studies with 213 PH patients who underwent PADN were included. Meta-analysis showed that after PADN, mPAP (mean difference [] -12.51, 95% confidence interval [CI] -17.74 to -7.27, P<0.00001) (mmHg) and PVR ( -5.17, 95% CI -7.70 to -2.65, P<0.0001) (Wood unit) decreased significantly, CO ( 0.59, 95% CI 0.32 to 0.86, P<0.0001) (L/min) and 6MWD ( 107.75, 95% CI 65.64 to 149.86, P<0.00001) (meter) increased significantly, and RV Tei index ( -0.05, 95% CI -0.28 to 0.17, P=0.63) did not change significantly. Also after PADN, the proportion of NYHA cardiac function grading (risk ratio 0.23, 95% CI 0.14 to 0.37, P<0.00001) III and IV decreased significantly.

Conclusion: This meta-analysis supports PADN as a potential new treatment for PH. Further high-quality randomized controlled studies are needed.
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http://dx.doi.org/10.21470/1678-9741-2020-0533DOI Listing
August 2022

Downregulation of HNRNPM inhibits cell proliferation and migration of hepatocellular carcinoma through MAPK/AKT signaling pathway.

Transl Cancer Res 2022 Jul;11(7):2135-2144

Department of Gastroenterology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Background: HNRNPM is reported to be involved in multiple malignancies, while the prognostic and biological role of HNRNPM in hepatocellular carcinoma remains still unknown.

Methods: Public databases and tissue microarrays were employed to identify the expression pattern and prognostic value of HNRNPM in hepatocellular carcinoma. CCK8, cell migration assays and western blot were taken advantage of to discover the biological role of HNRNPM in hepatocellular carcinoma. Western blot and bioinformatics analysis were used to reveal the potential signaling pathways of HNRNPM in hepatocellular carcinoma.

Results: High expression of HNRNPM was proved to be a poor independent prognostic factor for overall survival (OS) of hepatocellular carcinoma patients. Tissue microarrays and immunohistochemistry showed that HNRNPM protein level was upregulated in pancreatic cancer tissues compared with normal pancreas. Knockdown of HNRNPM suppressed significantly the capacities of proliferation and migration and alter epithelial mesenchymal transition of hepatocellular carcinoma cells. Downregulation of HNRNPM resulted in inhibition of the MAPK/AKT signaling pathway in hepatocellular carcinoma. Bioinformatics implied that HNRNPM might be a component of spliceosome to participate in hepatocellular carcinoma.

Conclusions: This paper identified high expression of HNRNPM was a poor independent prognostic factor for OS of hepatocellular carcinoma and could participate in proliferation and migration through MAPK/AKT signaling pathways.
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http://dx.doi.org/10.21037/tcr-21-2484DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9372219PMC
July 2022

Corrigendum: Relationship between c-reactive protein/serum albumin ratio, neutrophil/lymphocyte ratio, and ANCA-associated vasculitis activity: A retrospective single center cohort study.

Front Med (Lausanne) 2022 25;9:963919. Epub 2022 Jul 25.

Department of Rheumatology and Clinical Immunology, Jiangxi Provincial People's Hospital, Medical College of Nanchang University, Nanchang, China.

[This corrects the article DOI: 10.3389/fmed.2022.855869.].
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http://dx.doi.org/10.3389/fmed.2022.963919DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358650PMC
July 2022

Corrigendum to "SIRT2 regulates proliferation and chemotherapy response of MLL-ENL-driven acute myeloid leukemia".

Biochem Biophys Res Commun 2022 Oct 9;623:202-204. Epub 2022 Aug 9.

Department of Biochemistry and Molecular Cell Biology, Shanghai Jiao Tong University School of Medicine, 280 S. Chongqing Road, Shanghai, 200025, China. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2022.07.082DOI Listing
October 2022

A QSAR-ICE-SSD model prediction of the PNECs for alkylphenol substances and application in ecological risk assessment for rivers of a megacity.

Environ Int 2022 Sep 21;167:107367. Epub 2022 Jun 21.

United States Environmental Protection Agency, Gulf Ecosystem Measurement and Modeling Division, Gulf Breeze, Florida 32561, United States.

Alkylphenols (APs) are ubiquitous and generally present in higher residue levels in the environment. The present work focuses on the development of a set of in silico models to predict the aquatic toxicity of APs with incomplete/unknown toxicity data in aquatic environments. To achieve this, a QSAR-ICE-SSD model was constructed for aquatic organisms by combining quantitative structure-activity relationship (QSAR), interspecies correlation estimation (ICE), and species sensitivity distribution (SSD) models in order to obtain the hazardous concentrations (HCs) of selected APs. The research indicated that the keywords "alkylphenol" and "nonylphenol" were most commonly studied. The selected ICE models were robust (R: 0.70-0.99; p-value < 0.01). All models had a high reliability cross- validation success rates (>75%), and the HC predicted with the QSAR-ICE-SSD model was 2-fold than that derived with measured experimental data. The HC values demonstrated nearly linear decreasing trend from 2-MP to 4-HTP, while the decreasing trend from 4-HTP to 4-DP became shallower, indicates that the toxicity of APs to aquatic organisms increases with the addition of alkyl carbon chain lengths. The ecological risks assessment (ERA) of APs revealed that aquatic organisms were at risk from exposure to 4-NP at most river stations (the highest risk quotient (RQ) = 1.51), with the highest relative risk associated with 2.9% of 4-NP detected in 82.9% of the sampling sites. The targeted APs posed potential ecological risks in the Yongding and Beiyun River according to the mixture ERA. The potential application of QSAR-ICE-SSD models could satisfy the immediate needs for HC derivations without the need for additional in vivo testing.
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http://dx.doi.org/10.1016/j.envint.2022.107367DOI Listing
September 2022

Vaginal microbiota and personal risk factors associated with HPV status conversion-A new approach to reduce the risk of cervical cancer?

PLoS One 2022 9;17(8):e0270521. Epub 2022 Aug 9.

BGI Genomics, BGI-Shenzhen, Shenzhen, China.

Vaginal microbiota (VMB) is associated with changes in Human papilloma virus (HPV) status, which consequently influences the risk of cervical cancer. This association was often confounded by personal risk factors. This pilot research aimed to explore the relationship between vaginal microbiota, personal risk factors and their interactions with HPV status conversion to identify the vaginal microbiota that was associated with HPV clearance under heterogeneous personal risk factors. A total of 38 women participated by self-collecting a cervicovaginal mucus (CVM) sample that was sent for metagenomics sequencing. Most of the participants also filled in personal risk factors questionnaire through an eHealth platform and authorized the use of their previous HPV genotyping results stored in this eHealth platform. Based on the two HPV results, the participants were grouped into three cohorts, namely HPV negative, HPV persistent infection, and HPV status conversion. The relative abundance of VMB and personal factors were compared among these three cohorts. A correlation investigation was performed between VMB and the significant personal factors to characterize a robustness of the panel for HPV status change using R programming. At baseline, 12 participants were HPV-negative, and 22 were HPV-positive. Within one year, 18 women remained HPV-positive, 12 were HPV-negative and 4 participants showed HPV clearance. The factors in the eHealth questionnaire were systematically evaluated which identified several factors significantly associated with persistent HPV infection, including age, salary, history of reproductive tract infection, and the total number of sexual partners. Concurrent vaginal microbiome samples suggest that a candidate biomarker panel consisting of Lactobacillus gasseri, Streptococcus agalactiae, and Timona prevotella bacteria, which may be associated with HPV clearance. This pilot study indicates a stable HPV status-related vaginal microbe environment. To establish a robust biomarker panel for clinical use, larger cohorts will be recruited into follow-up studies.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270521PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362946PMC
August 2022

The Efficacy and Safety of Anlotinib in Extensive-Stage Small Cell Lung Cancer: A Multicenter Real-World Study.

Cancer Manag Res 2022 2;14:2273-2287. Epub 2022 Aug 2.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Purpose: Anlotinib, an antiangiogenic multi-target tyrosine kinase inhibitor (TKI), has shown favorable anticancer efficacy and acceptable safety in treating extensive-stage small cell lung cancer (ES-SCLC) in some clinical studies. This research aimed to explore the real-world efficacy and safety of anlotinib in ES-SCLC.

Methods: Pathologically confirmed ES-SCLC patients receiving anlotinib were enrolled for this retrospective study. The primary endpoint of this study was progression-free survival (PFS), and secondary endpoints included overall survival (OS), objective response rate (ORR), disease control rate (DCR), and adverse reactions.

Results: In total, 202 patients were included in this study. The median PFS of all patients was 4.8 months [95% confidence interval (CI): 3.9-5.7], and the median OS was 7.6 months (95% CI 6.5-8.7). Respectively, the overall ORR and DCR were 30.2% and 87.1%. The univariate and multivariate Cox regression analyses revealed that patients with Eastern Cooperative Oncology Group Performance Status (ECOG PS) ≤1, plus chemotherapy or immunotherapy, plus radiotherapy, and post-medication hypertension might have longer PFS and OS. The PFS and OS were significantly prolonged in combination group than that in monotherapy group [PFS 6.0 vs 3.6 months, hazards ratio (HR)=0.49, 95% CI 0.34-0.70, < 0.001; OS 9.2 vs 4.8 months, HR = 0.48, 95% CI 0.32-0.72, < 0.001]. The main treatment-related adverse reactions were generally tolerated. The incidence of adverse reactions in combination group was higher than that in monotherapy group (75.0% vs 52.6%, = 0.001). The most common adverse reaction was hypertension, followed by hand-foot syndrome and fatigue, regardless of monotherapy or combination group.

Conclusion: Anlotinib is effective and well tolerated in patients with ES-SCLC in the real-world. The clinical efficacy of anlotinib combined with chemotherapy or immunotherapy is better than that of monotherapy. Further investigations are needed for prospective studies with larger sample size.
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http://dx.doi.org/10.2147/CMAR.S364125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356751PMC
August 2022

The Bombyx mori Nucleopolyhedrovirus GP64 Retains the Transmembrane Helix of Signal Peptide to Contribute to Secretion across the Cytomembrane.

Microbiol Spectr 2022 Aug 8:e0191322. Epub 2022 Aug 8.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, People's Republic of China.

Bombyx mori nucleopolyhedrovirus (BmNPV) is the primary pathogen of silkworms that causes severe economic losses in sericulture. GP64 is the key membrane fusion protein that mediates budded virus (BV) fusion with the host cell membrane. Previously, we found that the n-region of the GP64 signal peptide (SP) is required for protein secretion and viral pathogenicity; however, our understanding of BmNPV GP64 remains limited. Here, we first reported that BmNPV GP64 retained its SP in the mature protein and virion in only host cells but did not retain in nonhost cells. Uncleaved SP mediates protein targeting to the cytomembrane or secretion in Bombyx mori cells. The exitance of the n-region extended the transmembrane helix length, which resulted in the cleavage site to be located in the helix structure and thus blocked cleavage from signal peptidase (SPase). Without the n-region, the protein fails to be transported to the cytomembrane, but this failure can be rescued by the cleavage site mutation of SP. Helix-breaking mutations in SP abolished protein targeting to the cytomembrane and secretion. Our results revealed a previously unrecognized mechanism by which SP of membrane fusion not only determines protein localization but also determines viral pathogenicity, which highlights the escape mechanism of SP from the cleavage by SPase. BmNPV is the primary pathogen of silkworms, which causes severe economic losses in sericulture. BmNPV and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) are closely related group I alphabaculoviruses, but they exhibit nonoverlapping host specificity. Recent studies suppose that GP64 is a determinant of host range, while knowledge remains limited. In this study, we revealed that BmNPV GP64 retained its SP in host cells but not in nonhost cells, and the SP retention is required for GP64 secretion across the cytomembrane. This is the first report that a type I membrane fusion protein retained its SP in mature proteins and virions. Our results unveil the mechanism by which SP GP64 escapes cleavage and the role of SP in protein targeting. This study will help elucidate an important mechanistic understanding of BmNPV infection and host range specificity.
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http://dx.doi.org/10.1128/spectrum.01913-22DOI Listing
August 2022

Expression and Clinical Significance of Serum Krüppel-Like Factor 7 (KLF7) in NSCLC Patients.

Comput Math Methods Med 2022 27;2022:9270789. Epub 2022 Jul 27.

Pathology Department, Hebei Chest Hospital, Shijiazhuang, Hebei 050000, China.

Nonsmall cell lung cancer (NSCLC) is a serious threat to the life and health of patients with high incidence rate and mortality. The present research was to assess the relationship between the serum Krüppel-like factor 7 (KLF7) level and the recurrence and metastasis of NSCLC patients. 150 patients with NSCLC treated by thoracoscopic radical resection of lung cancer in our hospital from January 2016 to February 2017 were selected. As the control group, 148 healthy people who went to the hospital for physical examination in the same period were screened. The expression levels of serum KLF7 in the observation group and the control group were compared and analyzed. According to the level of KLF7 expression, the patients in the observation group were divided into KLF7 high expression group (≥258.6 ng/L,  =75) and KLF7 low expression group (<258.6 ng/L,  =75). The 3-year recurrence and metastasis rate of patients in each group was compared and analyzed. It was found the concentration of serum KLF7 in peripheral blood of NSCLC (2.25 ± 0.65) ng/ml was significantly higher than that in healthy population (1.42 ± 0.38) ng/ml ( < 0.05). The expression level of serum KLF7 was not related to gender, age, smoking history, and tumor diameter of NSCLC patients ( > 0.05), but related to the degree of differentiation and TNM stage of NSCLC patients ( < 0.05). Univariate analysis showed that the degree of differentiation, TNM stage, and KLF7 were significantly correlated with 3-year recurrence and metastasis of NSCLC patients ( < 0.05). Cox regression analysis showed that low degree of differentiation, TNM stage IIIa, and KLF7 were independent risk factors for recurrence and metastasis in NSCLC patients in 3 years ( < 0.05). Taken together, the expression level of serum KLF7 in patients with NSCLC is significantly increased, which is an independent risk factor for recurrence and metastasis in 3 years, and is worthy of clinical application.
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http://dx.doi.org/10.1155/2022/9270789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348920PMC
August 2022

The relationship between ego depletion and work alienation in Chinese nurses: A network analysis.

Front Psychol 2022 22;13:915959. Epub 2022 Jul 22.

Department of Nursing, Air Force Medical University, Xi'an, China.

Objectives: To investigate the network structure of ego depletion and work alienation in Chinese nurses and identify bridge items to provide suggestions to reduce ego depletion and work alienation.

Methods: A total of 353 nurses from three hospitals were enrolled in our cross-sectional study by convenience sampling from June to October 2021 in China. They completed an online survey, which included the Sociodemographic Questionnaire, Nurses' Work Alienation Questionnaire, and Self-Regulating Fatigue Scale (SRF-S). The R packages qgraph, networktools, and bootnet were used to estimate the network model and calculate the indices.

Results: The correlation between ego depletion and work alienation was mainly positive. The correlation between "Sometimes I do not know what to do with the work instructions from my superiors" and "I have difficulties remembering things" was the strongest among the cross-community correlations ( = 0.14). The bridge strength centrality indices of "Sometimes I do not know what to do with the work instructions from my superiors," "I always feel like a loser" and "I have difficulties remembering things" were the highest ( = 3.15, 2.83, 1.43). The correlation stability coefficient of the centrality index was larger than 0.25.

Conclusion: Nurses' ego depletion and work alienation are correlated. "Sometimes I do not know what to do with the work instructions from my superiors," "I always feel like a loser" and "I have difficulties remembering things" act as bridges between ego depletion and work alienation communities, and should be the focus of nurses' psychological tests. Our study provides potential targets for interventions to reduce work alienation from the perspective of ego depletion.
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http://dx.doi.org/10.3389/fpsyg.2022.915959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355549PMC
July 2022

Class IIa histone deacetylase inhibition ameliorates acute kidney injury by suppressing renal tubular cell apoptosis and enhancing autophagy and proliferation.

Front Pharmacol 2022 22;13:946192. Epub 2022 Jul 22.

Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Expression and function of histone deacetylases (HDACs) vary with cell types and pathological conditions. Our recent studies showed that pharmacological targeting class IIa HDACs attenuated renal fibrosis, but the effect of class IIa HDAC inhibition on acute kidney injury (AKI) remains unknown. In this study, we found that four class IIa HDACs (4, 5, 7, 9) were highly expressed in the kidney of folic acid (FA) and ischemia/reperfusion (I/R)-induced AKI in mice. Administration of TMP269, a potent and selective class IIa HDAC inhibitor, improved renal function and reduced tubular cell injury and apoptosis, with concomitant suppression of HDAC4 and elevation of acetyl-histone H3. Mechanistical studies showed that TMP269 treatment inhibited FA and I/R-induced caspase-3 cleavage, Bax expression and p53 phosphorylation. Conversely, TMP269 administration preserved expression of E-cadherin, BMP7, Klotho and Bcl-2 in injured kidneys. Moreover, TMP269 was effective in promoting cellular autophagy as indicated by increased expression of Atg7, beclin-1, and LC3II, and promoted renal tubular cell proliferation as shown by increased number of proliferating cell nuclear antigen-positive cells and expression of cyclin E. Finally, blocking class IIa HDACs inhibited FA-and I/R-induced phosphorylation of extracellular signal-regulated kinases 1 and 2, and p38, two signaling pathways associated with the pathogenesis of AKI. Collectively, these results suggest that pharmacological inhibition of class IIa HDACs protects against AKI through ameliorating apoptosis, enhancing autophagy and promoting proliferation of renal tubular cells by targeting multiple signaling pathways.
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http://dx.doi.org/10.3389/fphar.2022.946192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354984PMC
July 2022

Insight into high-performance of La-Ce-MnOx oxides with different calcination temperatures for diesel soot combustion.

Environ Technol 2022 Aug 18:1-9. Epub 2022 Aug 18.

Hunan Provincial Key Laboratory of Xiangnan Rare-Precious Metals Compounds Research and Application, Chenzhou, People's Republic of China.

A series of La-Ce-MnOx catalysts derived from the precipitation of acetate salt and ammonium carbonate were calcined at different temperatures and applied for the catalytic oxidation of soot from diesel exhausts. The structure states of the as-prepared catalysts and catalytic behaviour for soot oxidation were studied by many characterization techniques such as XRD, XANES, N adsorption-desorption, TPR, O-TPD, XPS and TGA. XANES results display most Mn species are Mn when the samples are calcined at 500 and 600°C. However, for MCLa-700 and MCLa-800 catalysts, the predominance valence is 3+ ions. The MCLa-500 and MCLa-600 catalysts possess higher concentrations of surface active oxygen evidenced by H-TPR and soot-TPR. Therefore, the combustion rate of soot over MCLa-600 catalyst is remarkably increased with the T, T and T at 318°C, 360°C and 388°C under NOx atmosphere respectively.
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http://dx.doi.org/10.1080/09593330.2022.2109994DOI Listing
August 2022

Allogeneic haematopoietic stem cell transplantation with decitabine-containing preconditioning regimen in TP53-mutant myelodysplastic syndromes: A case study.

Front Oncol 2022 18;12:928324. Epub 2022 Jul 18.

Senior Department of Hematology, The Fifth Medical Centre of Chinese People's Liberation Army General Hospital, Beijing, China.

Myelodysplastic syndrome (MDS) with TP53 mutations has a poor prognosis after transplantation, and novel therapeutic means are urgently needed. Decitabine (Dec) monotherapy has demonstrated improved overall response rates in MDS and acute myeloid leukaemia, although these responses were not durable. This study aimed to preliminary evaluate the efficacy of a Dec-containing allogeneic haematopoietic stem cell transplantation (allo-HSCT) preconditioning regimen in TP53-mutant MDS. Nine patients with TP53-mutant myelodysplastic syndromes received the decitabine-containing preconditioning regimen and subsequent myeloablative allo-HCT between April 2013 and September 2021 in different centres. At a median follow-up of 42 months (range, 5 to 61 months), the overall survival (OS) was 89% (8/9), progression-free survival (PFS) was 89% (8/9), and relapse incidence was 11.1%. The incidence of severe acute (grade III-IV) graft-versus-host disease (GVHD) was 22.2% (2/9) and that of chronic moderate-to-severe GVHD was 11.1% (1/9). The 1-year GVHD-free/relapse-free survival (GRFS) was 56% (5/9). In conclusion, we found real-world clinical data that supports the use of a Dec-containing preconditioning regimen before allo-HSCT for possible improved outcomes in TP53-mutant MDS patients; there is therefore an urgent call for an in-depth exploration of the involved mechanism to confirm these preliminary findings.
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http://dx.doi.org/10.3389/fonc.2022.928324DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9339648PMC
July 2022

CircIPO7 Promotes Nasopharyngeal Carcinoma Metastasis and Cisplatin Chemoresistance by Facilitating YBX1 Nuclear Localization.

Clin Cancer Res 2022 Aug 2. Epub 2022 Aug 2.

Sun Yat-sen University Cancer Center, Guangzhou, China.

Purpose: Cisplatin-based chemotherapy effectively improves the distant-metastasis control in nasopharyngeal carcinoma (NPC), but approximately 30% of patients develop treatment failure due to chemoresistance. However, the underlying mechanisms remain poorly understood.

Experimental Design: CircRNA sequencing data were used to identify metastasis-specific circRNAs and the expression of circIPO7 was validated in NPC tissues as well as NPC cell lines by qRT-PCR. The whole transcriptional profile upon circIPO7 knockdown was applied to explore the biological function and regulatory mechanism, which were further confirmed by in vitro and in vivo metastasis/chemosensitivity assays. We also evaluated the value of circIPO7 expression in predicting NPC metastasis and cisplatin chemoresistance by analyzing a cohort of 183 NPC patients.

Results: In the current study, circIPO7, a novel circular RNA (circRNA), is found to be specifically overexpressed in NPC patients with distant metastasis. Knockdown of circIPO7 in NPC cells suppresses their metastasis and increases sensitivity to cisplatin treatment in vitro and in vivo. Mechanistically, circIPO7 binds to Y-box binding protein-1 (YBX1) protein in the cytoplasm and facilitates its phosphorylation at serine 102 (p-YBX1S102) by the kinase AKT, which further promotes YBX1 nuclear translocation and activates FGFR1, TNC, and NTRK1 transcription. Clinically, higher circIPO7 expression indicates unfavorable distant metastasis-free survival in NPC patients given cisplatin-based chemotherapy.

Conclusions: Altogether, this study identifies oncogenic circIPO7 as a prognostic marker after cisplatin-based chemotherapy and as a potential therapeutic target for overcoming metastasis and chemoresistance in NPC.
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http://dx.doi.org/10.1158/1078-0432.CCR-22-0991DOI Listing
August 2022

Security risk assessment and visualization study of key nodes of sea lanes: case studies on the Tsugaru Strait and the Makassar Strait.

Nat Hazards (Dordr) 2022 Jul 27:1-25. Epub 2022 Jul 27.

School of Marine Science and Technology, Tianjin University, Nankai District, 92 Weijin Road, Tianjin, 300072 People's Republic of China.

Key nodes of sea lanes are important hubs for global trade and cargo transportation and play important roles in ensuring the safety of maritime transportation and maintaining the stability of the global supply chain. The safety guarantee of key nodes of sea lanes is facing more risks and higher requirements currently because the global shipping industry is gradually recovering. This paper focuses on key nodes of sea lanes, conducting regional security risk assessment and risk spatial scale visualization. A set of security risk assessment and visualization study methods for key nodes of sea lanes is constructed, which includes constructing a security risk assessment index system of key nodes of sea lanes with 25 indicators selected from three risk categories (hazard, vulnerability and exposure, and mitigation capacity) and using geospatial analysis to form the multi-criteria spatial mapping layers and then creating comprehensive risk layers to realize the risk visualization in the strait area by weighted overlaying based on the combined weights calculated by Analytic Hierarchy Process and Grey Relational Analysis. After taking the Tsugaru Strait and Makassar Strait as case studies, the results show that the comprehensive risk layers can effectively present the spatial distribution of security risks of key nodes of sea lanes, reflecting the spatial changes of risk levels (i.e., very low, low, medium, high and very high) and the methods can precisely identify and analyze crucial factors affecting the security risk of key nodes. These findings may strengthen the risk prevention and improve the safety of the navigation environment in the strait to ensure the safety and stability of maritime trade.
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http://dx.doi.org/10.1007/s11069-022-05484-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326424PMC
July 2022

Macrophages in Renal Injury, Repair, Fibrosis Following Acute Kidney Injury and Targeted Therapy.

Front Immunol 2022 13;13:934299. Epub 2022 Jul 13.

Department of Nephrology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Acute kidney injury (AKI) is a renal disease with a high incidence and mortality. Currently, there are no targeted therapeutics for preventing and treating AKI. Macrophages, important players in mammalian immune response, are involved in the multiple pathological processes of AKI. They are dynamically activated and exhibit a diverse spectrum of functional phenotypes in the kidney after AKI. Targeting the mechanisms of macrophage activation significantly improves the outcomes of AKI in preclinical studies. In this review, we summarize the role of macrophages and the underlying mechanisms of macrophage activation during kidney injury, repair, regeneration, and fibrosis and provide strategies for macrophage-targeted therapies.
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http://dx.doi.org/10.3389/fimmu.2022.934299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326079PMC
August 2022

Burden, trends, and risk factors for breast cancer in China from 1990 to 2019 and its predictions until 2034: an up-to-date overview and comparison with those in Japan and South Korea.

BMC Cancer 2022 Jul 29;22(1):826. Epub 2022 Jul 29.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, China.

Background: The difference in epidemiological characteristics of breast cancer (BC) across countries is valuable for BC management and prevention. The study evaluated the up-to-date burden, trends, and risk factors of BC in China, Japan and South Korea during 1990-2019 and predicted the BC burden until 2034.

Methods: Data on incident cases, deaths, disability-adjusted life-years (DALYs) and age-standardized rate (ASR) of BC were extracted from the Global Burden of Disease Study 2019. Trend analysis and prediction until 2034 were conducted by estimated annual percentage change and a Bayesian age-period-cohort model, respectively. Besides, the attributable burden to BC risk factors was also estimated.

Results: In 2019, the number of BC incident cases, deaths and DALYs in China were 375,484, 96,306 and 2,957,453, respectively. The ASR of incidence increased, while that of death and DALYs decreased for Chinese females and Japanese and South Korean males during 1990-2019. High body-mass-index (BMI) was the largest contributor to Chinese female BC deaths and DALYs, while alcohol use was the greatest risk factor for Japanese and South Korean as well as Chinese males. The incident cases and deaths were expected to continue increase during 2020-2034 (except for Japanese female incident cases).

Conclusions: China had the greatest burden of BC among the three countries. Incident cases and deaths of BC were projected to increase over the next 15 years in China, particularly among Chinese males. Effective prevention and management strategies are urgently necessary for BC control in China.
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http://dx.doi.org/10.1186/s12885-022-09923-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334732PMC
July 2022

[One-step rapid enrichment and detection of malachite green in aquaculture water based on metal-organic framework hydrogel].

Se Pu 2022 Aug;40(8):721-729

Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, Beijing 100141, China.

Malachite green is a triphenylmethane compound, which has a good effect on disease prevention and control in the breeding of aquatic products, but it is a prohibited drug because it is detrimental to human health. Owing to the low content of target malachite green and complex components in the actual sample, simultaneously achieving good enrichment, high sensitivity, convenience, and rapidity detecting is difficult. Metal-organic framework (MOF) has a multidimensional network structure, good stability, and large specific surface area, and has broad application prospects in adsorption. However, the small particle size of MOF materials and the difficulty of recycling hinder their development. The hydrogel has a three-dimensional network structure that can encapsulate the MOF nanomaterials in the network, enhancing adsorption performance and facilitating separation from the adsorbed solution. In this study, MOF materials were prepared, and hydrogel doped with MOF nanomaterials (polyacrylamide-sodium alginate/metal-organic framework, PAAM-SA/MOF) was used to investigate the adsorption of malachite green in aquaculture water. The transmission electron microscopy was used to characterize MOF nanomaterials, scanning electron microscopy was used to examine the morphology and structure of the hydrogels before and after adsorption of malachite green, representing the successful synthesis of adsorbent materials with excellent properties. The mechanical properties of the hydrogels were investigated using a tensile testing machine, with a maximum tensile strain of up to 300%, without breaking and failing to remove when separated from the solution to be tested. The pore size of PAAM-SA/MOF hydrogel is considerably smaller than that of PAAM-SA, which is beneficial to increase the specific surface area of the adsorbent and thus improve the adsorption performance. A series of optimizations were performed on the adsorption conditions of the hydrogel adsorbent and the optimized conditions were obtained as follows: the amount of adsorbent used was 0.1 g, adsorption time was 5 h, the pH of malachite green solution was 9, the adsorption temperature was 40 ℃, and the initial concentration of malachite green solution was 100 mg/L. Under these conditions the adsorption efficiency could reach up to 97%. Furthermore, the adsorbed malachite green was eluted with organic solvents of various polarities, and the highest desorption efficiency was achieved when acetonitrile with higher polarity was used as the eluent. Simultaneously, the eluent volume was optimized, with 2 mL acetonitrile added to the malachite green-enriched hydrogel adsorbent to achieve the highest desorption efficiency of 99%. The enriched sample was separated using a Dionex Bonded Silica Products C column (50 mm×2.1 mm, 3 μm), and eluted with an ammonium acetate-acetonitrile solvent system. The results showed that the limit of detection (LOD, =3) was 0.083 μg/L, the limit of quantification (LOQ, =10) was 0.25 μg/L, and the spiked recoveries of malachite green at high, medium, and low levels were 84.8%-118.1% with the relative standard deviations less than 5.1%. The pretreatment is simplified using this approach and combines the respective advantages of MOF and hydrogel to enable one-step enrichment of malachite green in aquaculture water. The additional MOF material can exert good adsorption in the hydrogel system, which solves the problem of low recovery of traditional MOF materials caused by tiny particle size, facilitating direct extraction after adsorption, and also solves the problem of low adsorption efficiency of pure hydrogel, improving the overall adsorption efficiency and recyclability. The actual sample test shows that the new hydrogel adsorption material can be used to extract and detect trace malachite green in aquaculture water. It is a novel, fast and convenient pretreatment approach with great potential in food detection.
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http://dx.doi.org/10.3724/SP.J.1123.2022.04019DOI Listing
August 2022

Association of serum creatinine with hepatic steatosis and fibrosis: a cross-sectional study.

BMC Gastroenterol 2022 Jul 27;22(1):358. Epub 2022 Jul 27.

Department of Gastroenterology, The Second Affiliated Hospital of Xi'an Jiaotong University, NO.157 Xiwu Road, Xi'an, 710004, Shaanxi, China.

Background: Recent studies have shown that chronic kidney disease (CKD) prevalence is significantly higher in patients with hepatic steatosis (HS); however, it remains unclear whether HS is associated with serum creatinine (SCr). We aimed to explore the association between SCr levels and HS in a Chinese population.

Methods: We performed a cross-sectional study among 56,569 Chinese individuals. SCr level, other clinical and laboratory parameters, abdominal ultrasound and noninvasive fibrosis scores were extracted, and the fibrosis 4 score (FIB-4) was calculated.

Results: A total of 27.1% of the subjects had HS. After 1:1 propensity score matching (PSM) according to sex and age, we included 13,301 subjects with HS and 13,301 subjects without HS. SCr levels were significantly higher in the HS group than in the non-HS group [73.19 ± 15.14(μmoI/L) vs. 71.75 ± 17.49(μmoI/L), p < 0.001]. Univariate and multivariate regression analyses showed a positive association between SCr and the prevalence of HS. Stepwise regression analysis showed that the association between SCr and HS was independent of other metabolic syndrome components. The prevalence of HS increased significantly with increasing SCr levels. Metabolism-related indicators and liver enzymes were significantly higher in the HS group than in the non-HS group; furthermore, these parameters increased with increasing SCr levels. FIB-4 was significantly higher in the HS group than in the non-HS group but did not show an increasing trend with increasing SCr levels.

Conclusions: Our results showed an independent association between SCr level and HS risk in a Chinese population.
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http://dx.doi.org/10.1186/s12876-022-02437-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327226PMC
July 2022

Determination of pesticides and their degradation products in sediment samples by accelerated solvent extraction and solid-phase extraction with high-performance liquid chromatography-high-resolution mass spectrometry.

Anal Sci 2022 Jul 27. Epub 2022 Jul 27.

Shandong Province City Water Supply and Drainage Water Quality Monitoring Center, Jinan, 250013, Shandong, People's Republic of China.

A new sensitive and selective methods was developed to quantify different types of pesticides and their degradation products in sediment. The method developed was optimized and modified based on the accelerated solvent extraction, followed by the solid-phase extraction clean-up technique. High-performance liquid chromatography coupled with mass spectrometry was used for analysis. The influence of various parameters on the extraction process was investigated, including the extraction temperature, extraction solvent, purification column and purification solvent, etc. Under the optimal conditions, the relative recoveries of the pesticides and their degradation products ranged from 80 to 106% for spiked blank sediment and environmental sediment samples with relative standard deviations of 1-9%. The method displayed low method detection limits for both sediment matrices and achieved good linearity over the tested range of concentrations. The physical and chemical properties of sediment showed that high content of sediment water content and humic acid would affect the extraction efficiency of sample pretreatment. The method was applied to environmental sediment to quantify pesticide residues in the samples. Based on the instrument and method performance validation results, the developed methods can be applied in environmental pesticide residue analysis, thus providing a scientific method for the detection of sediment samples.
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http://dx.doi.org/10.1007/s44211-022-00165-9DOI Listing
July 2022

Exploration of Mycotoxin Accumulation and Transcriptomes of Different Wheat Cultivars during Infection.

Toxins (Basel) 2022 07 13;14(7). Epub 2022 Jul 13.

SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

is one of the most devastating diseases of wheat worldwide, and can cause Fusarium head blight (FHB). infection and mycotoxin production mainly present in wheat and can be influenced by environmental factors and wheat cultivars. The objectives of this study were to examine the effect of wheat cultivars and interacting conditions of temperature and water activity () on mycotoxin production by two strains of and investigate the response mechanisms of different wheat cultivars to infection. In this regard, six cultivars of wheat spikes under field conditions and three cultivars of post-harvest wheat grains under three different temperature conditions combined with five water activity () conditions were used for infection in our studies. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis showed significant differences in the concentration of Fusarium mycotoxins deoxynivalenol (DON) and its derivative deoxynivalenol-3-glucoside (D3G) resulting from wheat cultivars and environmental factors. Transcriptome profiles of wheat infected with revealed the lower expression of disease defense-factor-related genes, such as mitogen-activated protein kinases (MAPK)-encoding genes and hypersensitivity response (HR)-related genes of infected Annong 0711 grains compared with infected Sumai 3 grains. These findings demonstrated the optimal temperature and air humidity resulting in mycotoxin accumulation, which will be beneficial in determining the conditions of the relative level of risk of contamination with FHB and mycotoxins. More importantly, our transcriptome profiling illustrated differences at the molecular level between wheat cultivars with different FHB resistances, which will lay the foundation for further research on mycotoxin biosynthesis of and regulatory mechanisms of wheat to .
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http://dx.doi.org/10.3390/toxins14070482DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318452PMC
July 2022

Mycotoxins in Tea (( (L.) Kuntze)): Contamination and Dietary Exposure Profiling in the Chinese Population.

Toxins (Basel) 2022 07 1;14(7). Epub 2022 Jul 1.

SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Shanghai 200030, China.

Tea is popular worldwide with multiple health benefits. It may be contaminated by the accidental introduction of toxigenic fungi during production and storage. The present study focuses on potential mycotoxin contamination in tea and the probable dietary exposure assessments associated with consumption. The contamination levels for 16 mycotoxins in 352 Chinese tea samples were determined by ultra-performance liquid chromatography-tandem mass spectrometry. Average concentrations of almost all mycotoxins in tea samples were below the established regulations, except for ochratoxin A in the dark tea samples. A risk assessment was performed for the worst-case scenarios by point evaluation and Monte Carlo assessment model using the obtained mycotoxin levels and the available green, oolong, black, and dark tea consumption data from cities in China. Additionally, we discuss dietary risk through tea consumption as beverages and dietary supplements. In conclusion, there is no dietary risk of exposure to mycotoxins through tea consumption in the Chinese population.
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http://dx.doi.org/10.3390/toxins14070452DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318285PMC
July 2022

Effects of a Dark Septate Fungal Endophyte on the Growth and Physiological Response of Seedlings to Drought in an Epiphytic Orchid.

Front Microbiol 2022 8;13:961172. Epub 2022 Jul 8.

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Dark septate endophytes (DSE) are a group of facultative biotrophic root-colonizing fungi that live within a plant for a part of their life cycle without causing any apparent, overt negative effects. These fungi have been found in >600 different plant species, including orchids. Although the precise ecological functions of dark septate fungal endophytes are not yet well understood, there is increasing evidence that they enhance host growth and nutrient acquisition, and improve the plant's ability to tolerate biotic and abiotic stresses. In this research, we tested the effects of a DSE isolated from the roots of the epiphytic orchid on the growth and drought tolerance of orchid seedlings. Our results showed that addition of DSE inoculum significantly enhanced biomass of seedlings and increased the activities of drought resistance related enzymes and the accumulation of osmoregulatory substances. These results suggest that DSE can fulfill important ecological functions in stressful environments and potentially play an important role in the life cycle of epiphytic orchids.
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http://dx.doi.org/10.3389/fmicb.2022.961172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9304953PMC
July 2022

Ang2-Targeted Combination Therapy for Cancer Treatment.

Front Immunol 2022 8;13:949553. Epub 2022 Jul 8.

Institute of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang, China.

Angiopoietin-2 (Ang2), a member of the angiopoietin family, is widely involved in the process of vascular physiology, bone physiology, adipose tissue physiology and the occurrence and development of inflammation, cardiac hypertrophy, rheumatoid, tumor and other diseases under pathological conditions. Proliferation and metastasis of cancer largely depend on angiogenesis. Therefore, anti-angiogenesis has become the target of tumor therapy. Due to the Ang2 plays a key role in promoting angiogenesis and stability in vascular physiology, the imbalance of its expression is an important condition for the occurrence and development of cancer. It has been proved that blocking Ang2 can inhibit the growth, invasion and metastasis of cancer cells. In recent years, research has been constantly supplemented. We focus on the mechanisms that regulate the expression of Ang2 mRNA and protein levels in different cancers, contributing to a better understanding of how Ang2 exerts different effects in different cancers and stages, as well as facilitating more specific targeting of relevant molecules in cancer therapy. At the same time, the importance of Ang2 in cancer growth, metastasis, prognosis and combination therapy is pointed out. And finally, we will discuss the current investigations and future challenges of combining Ang2 inhibition with chemotherapy, immunotherapy, and radiotherapy to increase its efficacy in cancer patients. This review provides a theoretical reference for the development of new targets and effective combination therapy strategies for cancer treatment in the future.
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http://dx.doi.org/10.3389/fimmu.2022.949553DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9305611PMC
July 2022

ACSL4 is essential for radiation-induced intestinal injury by initiating ferroptosis.

Cell Death Discov 2022 Jul 22;8(1):332. Epub 2022 Jul 22.

Department of Radiation Oncology, Institute of Oncology, Affiliated Hospital, Jiangsu University, Zhenjiang, Jiangsu Province, China.

Lipid peroxidation-induced ferroptosis is a newly recognized type of programmed cell death. With the method of RNA sequencing, we found that irradiation (IR) markedly increased the expression of ferroptosis promotive genes, whereas reduced the expression of ferroptosis suppressive genes in murine intestine tissues, when compared with those of liver and lung tissues. By using ferroptosis inducer RSL-3 and inhibitor liproxstatin-1, we found that ferroptosis is essential for IR-induced intestinal injury. Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) is an important component for ferroptosis execution, and we found that ACSL4 expression was significantly upregulated in irradiated intestine tissues, but not in liver or lung tissues. Antibacterial and antifungal regents reduced the expression of ASCL4 and protected against tissue injury in irradiated intestine tissues. Further studies showed that troglitazone, a ACSL4 inhibitor, succeeded to suppresses intestine lipid peroxidation and tissue damage after IR.
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http://dx.doi.org/10.1038/s41420-022-01127-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307849PMC
July 2022

A prospective study of total urinary deoxynivalenol in adolescents in Shanghai, China.

Chemosphere 2022 Jul 18:135727. Epub 2022 Jul 18.

CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address:

There is growing interest in deoxynivalenol (DON) exposure during puberty because experimental evidence shows that DON-exposed to adolescents are more sensitive to DON and have limited detoxification ability. Nevertheless, there have been few surveys of DON exposure for adolescents in China. Furthermore, little is known about the effects of collection times on risk exposure. In the study, we estimated the risk of DON exposure for adolescents in Shanghai and explore the effects of collection time, areas, sex and BMI on intake estimates. A total of 315 adolescents aged 14-16 years, including 161 boys and 154 girls, were recruited. Urine samples were collected for three consecutive days and digested with β-glucuronidase and then purified using a DON-immunoaffinity column (IAC). Total deoxynivalenol levels were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in combination with a stable isotope dilution assay (SIDA). DON was detected in 945 morning urine samples taken from 315 individuals for three consecutive days. The mean estimated dietary intake of DON did not exceed the tolerable daily intake (TDI) of l μg/kg·bw/day, showing that exposure of adolescents in Shanghai is not of concern. However, 10-20% of probable daily intake (PDI) values exceed the TDI, indicating potential adverse effects. In addition, the DON concentration at the population level did not differ for urine samples collected at different times except for those of overweight adolescents. Therefore, assessment of exposure to DON by monitoring the morning urine of a healthy adolescent, except for overweight people, provides an appropriate estimate of exposure and related risk at the population level, but intake estimates for individuals are uncertain; these could be used to assess exposure of adolescents to DON rapidly and effectively for epidemiological investigations.
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http://dx.doi.org/10.1016/j.chemosphere.2022.135727DOI Listing
July 2022

Multi-Omics Integration Reveals the Crucial Role of in the Inflammatory Immune Microenvironment in Head and Neck Squamous Cell Carcinoma.

Microbiol Spectr 2022 Jul 11:e0106822. Epub 2022 Jul 11.

Department of Experimental Research, State Key Laboratory of Oncology in South China; Collaborative Innovation Center of Cancer Medicine; Sun Yat-sen Universitygrid.12981.33 Cancer Center, Sun Yat-sen University, Guangzhou, China.

The tumor microbiome is believed to have a profound impact on tumor progression owing to its local colonization in the tumor microenvironment (TME). Using the Cancer Microbiome Atlas (TCMA), a database of curated, decontaminated microbial profiles for 3,689 oropharyngeal, esophageal, gastrointestinal, and colorectal tissue samples from 1,772 patients, we conducted a comprehensive multi-omics analysis to reveal microbial signatures among various cancers and the potential mechanisms involved in tumor progression of head and neck squamous cell carcinoma (HNSC). We found that compared with other cancer types, the tumor-resident microbiome of HNSC accounted for the highest bacterial abundance and strongest association with host TME signatures. was found to be enriched in HNSC tissues, which was associated with an increased inflammatory effect and inferior prognosis. Moreover, we revealed that the microbiota-associated inflammatory TME was attributed to the competing endogenouse RNA (ceRNA) network and chromatin accessibility. Studies on revealing the composition and potential mechanisms of the tumor microbiome are still at an initial stage. We uncovered the potential contribution of the tumor-resident microbiota on the immunosuppressive microenvironment in HNSC, which will provide a new perspective for tumor microbiome research and yield valuable insights into the clinical management of HNSC.
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http://dx.doi.org/10.1128/spectrum.01068-22DOI Listing
July 2022

[A case-control study of preserving the shape of iliac bone and traditional iliac osteotomy].

Zhongguo Gu Shang 2022 Jul;35(7):625-9

The Second Hospital of Tangshan, Tangshan 063000, Hebei, China.

Objective: To compare and study the traditional iliac bone extraction and iliac crest preserving bone extraction, and to observe their curative effects.

Methods: From January 2016 to December 2018, 50 patients were treated with traditional iliac bone extraction and iliac crest preserving iliac bone extraction, with 25 cases in each group. In the traditional iliac bone extraction group, there were 18 males and 7 females, aged (42.0±7.9) years, hospitalized for (20.0±5.5) days and followed up for (13.68±1.60) months. There were 16 males and 9 females in the iliac crest preserving osteotomy group, aged (44.0±8.2) years, hospitalized for (21.0±6.5) days and followed up for (14.04±1.54) months. The operation time, intraoperative bleeding, postoperative pain visual analgue scale(VAS), fracture healing and complications were recorded and compared between the two groups.

Results: In the traditional iliac bone extraction group, the operation time was (16.20±2.51) min, the amount of bleeding was (63.20±17.73) ml, the VAS score on the first day after operation was 4.72±1.21, and the fracture healing time was (4.84±0.90) months. In the iliac crest preserving osteotomy group, the operation time was (16.24±3.00) min, the amount of bleeding was (62.80±18.14) ml, the VAS score was 4.80±1.29 on the first day after operation, and the fracture healing time was (4.68±0.80) months. There was no significant difference in operation time, bleeding volume, pain VAS on the first day after operation and fracture healing time between the two groups(>0.05).

Conclusion: Compared with the traditional iliac bone removal surgery, this bone removal method of preserving the appearance of iliac bone is simple and convenient. While ensuring the amount of bone removal, it retains the appearance of iliac crest as much as possible and reduces local discomfort symptoms after operation. It is worth popularizing.
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http://dx.doi.org/10.12200/j.issn.1003-0034.2022.07.006DOI Listing
July 2022
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