Publications by authors named "Na Jiang"

162 Publications

Predictive Analysis and Evaluation Model of Chronic Liver Disease Based on BP Neural Network with Improved Ant Colony Algorithm.

J Healthc Eng 2021 15;2021:3927551. Epub 2021 Nov 15.

Department of Gastroenterology, Hankou Hospital, Wuhan 430012, Hube, China.

Timely prediction of the mechanism and characteristics of chronic liver disease using next-generation information technology is an effective way to improve the diagnosis rate of chronic liver disease. In this paper, we have proposed a modified backpropagation (BP) neural network with improved ant colony optimization algorithm to process multiple index attribute items describing chronic liver disease and construct a chronic liver disease assessment model. The proposed model is very effective in detecting chronic liver disease on time with acceptable level of accuracy and precision ratio. To verify these claims, the proposed scheme is checked experimentally where 125 groups of 20-dimensional medical test index data items of patients with chronic liver disease were analyzed. Moreover, 13-dimensional index items were preferentially selected as test index attribute items with high sensitivity to chronic liver disease using well-known ROC curves. The 13-dimensional index items were reduced to 5-dimensional comprehensive data items by principal component analysis. The proposed neural network-based model was trained with 115 sets of test indicator sample sets, and the remaining 10 sets of sample sets were used as test samples. Compared with the original 20-dimensional data as the neural network input, the proposed model not only reduces the complexity but also improves the prediction accuracy by 15.07%.
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http://dx.doi.org/10.1155/2021/3927551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8608507PMC
November 2021

Application of dual carbon-bromine stable isotope analysis to characterize anaerobic micro-degradation mechanisms of PBDEs in wetland bottom-water.

Water Res 2021 Nov 9;208:117854. Epub 2021 Nov 9.

Navigation College, Dalian Maritime University, Dalian 116026, China.

Polybrominated diphenyl ethers (PBDEs), one kind of persistent organic pollutants, were widely detected in coastal wetlands. Microbial reductive debromination is one of the most important attenuation processes for PBDEs in anaerobic environment, whereas the underlying reaction mechanisms remain elusive. Dual-element stable isotope analysis was recently recognized to distinguish different reaction mechanism for degradation of organic pollutants. In this study, the dual carbon-bromine isotope effects associated with the anaerobic microbial degradation were first investigated to characterize the reaction mechanisms for BDE-47 and BDE-153. Presence of lower brominated congeners indicated stepwise debromination as the main degradation pathway, with the preferential removal of bromine in para position > meta/ortho position. The pronounced isotope fractionation was observed for both carbon and bromine, with similar carbon (ε) and bromine isotope enrichment factor (ε) between BDE-47 (ε = -5.98‰, ε = -2.44‰) and BDE-153 (ε = -5.57‰, ε = -2.06‰) during the microbial degradation. Compared to ε and ε, the correlation of carbon and isotope effects (Λ = ΔδBr/ΔδC) was almost the same between BDE-47 (0.436) and BDE-153 (0.435), indicating the similar reaction mechanism. The calculated carbon and bromine apparent kinetic isotope effects (AKIE and AKIE) were 1.0773 and 1.0098 for BDE-47 and 1.0716 and 1.0125 for BDE-153, within range reported for degradation of halogenated compounds following nucleophilic substitution. Combination analysis of degradation products, Λ and AKIE, all the results pointed to that the anaerobic reductive debromination of BDE-47 and BDE-153 followed the nucleophilic aromatic substitution, with the addition of cofactor to the benzene ring concomitant with dissociation of carbon-bromine bond via the inner-sphere electron transfer, and the cleavage of C-Br bond was the rate-determining step. This study contributed to the development of dual carbon-bromine isotope analysis as a robust approach to probe the fate of PBDEs in contaminated sites.
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http://dx.doi.org/10.1016/j.watres.2021.117854DOI Listing
November 2021

Tandem Mass Tag labelling quantitative acetylome analysis of differentially modified proteins during mycoparasitism of Clonostachys chloroleuca 67-1.

Sci Rep 2021 Nov 17;11(1):22383. Epub 2021 Nov 17.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Lysine acetylation (Kac) is an important post-translational modification (PTM) of proteins in all organisms, but its functions have not been extensively explored in filamentous fungi. In this study, a Tandem Mass Tag (TMT) labelling lysine acetylome was constructed, and differentially modified Kac proteins were quantified during mycoparasitism and vegetative growth in the biocontrol fungus Clonostachys chloroleuca 67-1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 1448 Kac sites were detected on 740 Kac proteins, among which 126 sites on 103 proteins were differentially regulated. Systematic bioinformatics analyses indicate that the modified Kac proteins were from multiple subcellular localizations and involved in diverse functions including chromatin assembly, glycometabolism and redox activities. All Kac sites were characterized by 10 motifs, including the novel CxxKac motif. The results suggest that Kac proteins may have effects of broadly regulating protein interaction networks during C. chloroleuca parasitism to Sclerotinia sclerotiorum sclerotia. This is the first report of a correlation between Kac events and the biocontrol activity of C. chloroleuca. Our findings provide insight into the molecular mechanisms underlying C. chloroleuca control of plant fungal pathogens regulated by Kac proteins.
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http://dx.doi.org/10.1038/s41598-021-01956-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599485PMC
November 2021

Lipophagy deficiency exacerbates ectopic lipid accumulation and tubular cells injury in diabetic nephropathy.

Cell Death Dis 2021 10 30;12(11):1031. Epub 2021 Oct 30.

Department of Nephrology, The Second Xiangya Hospital, Central South University, Key Laboratory of Kidney Disease and Blood Purification, Changsha, Hunan, China.

Autophagy-mediated lipotoxicity plays a critical role in the progression of diabetic nephropathy (DN), but the precise mechanism is not fully understood. Whether lipophagy, a selective type of autophagy participates in renal ectopic lipid deposition (ELD) and lipotoxicity in the kidney of DN is unknown. Here, decreased lipophagy, increased ELD and lipotoxcity were observed in tubular cells of patients with DN, which were accompanied with reduced expression of AdipoR1 and p-AMPK. Similar results were found in db/db mice, these changes were reversed by AdipoRon, an adiponectin receptor activator that promotes autophagy. Additionally, a significantly decreased level of lipophagy was observed in HK-2 cells, a human proximal tubular cell line treated with high glucose, which was consistent with increased lipid deposition, apoptosis and fibrosis, while were partially alleviated by AdipoRon. However, these effects were abolished by pretreatment with ULK1 inhibitor SBI-0206965, autophagy inhibitor chloroquine and enhanced by AMPK activator AICAR. These data suggested by the first time that autophagy-mediated lipophagy deficiency plays a critical role in the ELD and lipid-related renal injury of DN.
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http://dx.doi.org/10.1038/s41419-021-04326-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8557213PMC
October 2021

NLRP3 Inflammasome: A New Target for Prevention and Control of Osteoporosis?

Front Endocrinol (Lausanne) 2021 27;12:752546. Epub 2021 Sep 27.

The First Clinical Medical College of Lanzhou University, Lanzhou, China.

Osteoporosis is a systemic bone metabolism disease that often causes complications, such as fractures, and increases the risk of death. The nucleotide-binding oligomerization domain-like-receptor family pyrin domain-containing 3 (NLRP3) inflammasome is an intracellular multiprotein complex that regulates the maturation and secretion of Caspase-1 dependent proinflammatory cytokines interleukin (IL)-1β and IL-18, mediates inflammation, and induces pyroptosis. The chronic inflammatory microenvironment induced by aging or estrogen deficiency activates the NLRP3 inflammasome, promotes inflammatory factor production, and enhances the inflammatory response. We summarize the related research and demonstrate that the NLRP3 inflammasome plays a vital role in the pathogenesis of osteoporosis by affecting the differentiation of osteoblasts and osteoclasts. IL-1β and IL-18 can accelerate osteoclast differentiation by expanding inflammatory response, and can also inhibit the expression of osteogenic related proteins or transcription factors. and experiments showed that the overexpression of NLRP3 protein was closely related to aggravated bone resorption and osteogenesis deficiency. In addition, abnormal activation of NLRP3 inflammasome can not only produce inflammation, but also lead to pyroptosis and dysfunction of osteoblasts by upregulating the expression of Caspase-1 and gasdermin D (GSDMD). In conclusion, NLRP3 inflammasome overall not only accelerates bone resorption, but also inhibits bone formation, thus increasing the risk of osteoporosis. Thus, this review highlights the recent studies on the function of NLRP3 inflammasome in osteoporosis, provides information on new strategies for managing osteoporosis, and investigates the ideal therapeutic target to treat osteoporosis.
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http://dx.doi.org/10.3389/fendo.2021.752546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502943PMC
September 2021

Research on Smart Healthcare Services: Based on the Design of APP Health Service Platform.

J Healthc Eng 2021 29;2021:9922389. Epub 2021 Sep 29.

School of History and Culture, Northeast Normal University, Changchun, Jilin 130011, China.

With the development of information technology, big data, and cloud computing, the concept of smart healthcare has gradually become more and more important. Compared with the traditional healthcare service, the new model, health service platform, is becoming increasingly popular and convenient. The use of wearable monitoring devices and some APPs is improving the health monitoring efficacy and effectiveness. To improve and facilitate the smart healthcare services, an effective and convenient app health service platform is needed urgently to serve the older and younger. Based on the above, this paper elaborates the principles of health service system and health information perception terminal design of the APP health service platform.
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http://dx.doi.org/10.1155/2021/9922389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494541PMC
September 2021

Unmarked gene editing in Clavibacter michiganensis using CRISPR/Cas9 and 5-fluorocytosine counterselection.

Mol Plant Microbe Interact 2021 Sep 20. Epub 2021 Sep 20.

China Agricultural University, 34752, Plant Pathology, Yuanmingyuan West Rd. Haidian District, Beijing, China, 100193;

Plant pathogenic bacteria in the genus Clavibacter are important quarantine species that cause considerable economic loss worldwide. The development of effective gene editing techniques and additional selectable markers is essential to expedite gene functional analysis in this important Gram-positive genus. The current study details a highly efficient unmarked CRISPR/Cas9-mediated gene editing system in Clavibacter michiganensis (Cm), which couples the expression of cas9 and sgRNA with homology-directed repair templates and the negative selectable marker codA::upp within a single plasmid. Initial experiments indicated that CRISPR/Cas9-mediated transformation could be utilized for both site directed mutagenesis, in which an A to G point mutation was introduced at the 128th nucleotide of the Cm rpsL gene to generate a streptomycin resistant mutant, and complete gene knockout, in which the deletion of the Cm celA or katA genes resulted in transformants that lacked cellulase and catalase activity, respectively. In subsequent experiments the introduction of the codA::upp cassette into the transformation vector facilitated the counterselection of unmarked transformants by incubation in the absence of the selective antibiotic, followed by plating on M9 agar containing 100 μg/ml 5-fluorocytosine (5-FC), in which an unmarked katA mutant lacking the transformation vector was recovered. Compared to conventional homologous recombination, the unmarked CRISPR/Cas9-mediated system was more useful and convenient as it allowed the template plasmid to be reused repeatedly to facilitate the editing of multiple genes, which constitutes a major advancement that could revolutionize research into Cm and other Clavibacter species.
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http://dx.doi.org/10.1094/MPMI-07-21-0179-TADOI Listing
September 2021

A new acylated iridoid and other chemical constituents from and their biological activities.

Nat Prod Res 2021 Aug 3:1-8. Epub 2021 Aug 3.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, P. R. China.

A new acylated iridoid, valejatadoid H (), along with fourteen known compounds, were obtained from the -BuOH extract of the roots and rhizomes of , and their structures were elucidated by various spectroscopic methods. Among them, compounds , and exhibited potent inhibition on NO production, with IC values of 4.21, 6.08 and 20.36 μM, respectively. In addition, compounds and showed anti-influenza virus activities, among which compound exhibited significant effect with an IC value of 0.99 μM.
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http://dx.doi.org/10.1080/14786419.2021.1961255DOI Listing
August 2021

Metal-Organic Framework-Derived ZnSe- and CoSe-Filled Porous Nitrogen-Doped Carbon Nanocubes Interconnected by Reduced Graphene Oxide for Sodium-Ion Battery Anodes.

Inorg Chem 2021 Aug 15;60(15):11693-11702. Epub 2021 Jul 15.

College of Chemistry and Materials Science, Sichuan Normal University, Chengdu 610066, P. R. China.

Transition-metal selenides have been considered as one of the most promising anode materials for sodium-ion batteries (SIBs) due to their high theoretical capacity and excellent rate performance. However, rapid capacity decay and poor cycling stability limit their practical application as the anode for SIBs. Carbon coating is one of the most effective ways to solve the above problems, but the thickness and uniformity of the coating layer are difficult to control. Herein, we successfully synthesize metal-organic framework (MOF)-derived porous N-doped carbon nanocubes homogeneously filled with ZnSe and CoSe and interconnected by reduced graphene oxide ([email protected]@rGO). [email protected]@rGO with more active sites and the synergistic effect of the ZnSe and CoSe heterojunction can enhance the sodium storage performance. The porous carbon nanocubes effectively prevent the agglomeration of active particles, and the rGO acting as a carbon network can significantly buffer the inevitable volume changes. At the same time, carbon nanocubes and the rGO are interconnecting to form a conductive network to accelerate electron transfer. Based on the aforementioned advantages, the [email protected]@rGO electrode shows an excellent sodium storage performance. Our investigation opens up a new horizon for the rational design of transition-metal selenide anodes for SIBs with a unique structure.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01807DOI Listing
August 2021

Nonstructural Protein NSs Hampers Cellular Antiviral Response through LSm14A during Severe Fever with Thrombocytopenia Syndrome Virus Infection.

J Immunol 2021 07 9;207(2):590-601. Epub 2021 Jul 9.

Center for Public Health Research, Medical School, Nanjing University, Nanjing, People's Republic of China;

The nonstructural protein (NSs) of severe fever with thrombocytopenia syndrome virus (SFTSV) plays multiple functions in the virus life cycle. Proteomic screening for host proteins interacting with NSs identified the cellular protein LSm14A. LSm14A, a member of the LSm family involved in RNA processing in the processing bodies, binds to viral RNA or synthetic homolog and mediates IFN regulatory factor 3 activation and IFN-β induction. NSs interacted with and colocalized with LSm14A, and this interaction effectively inhibited downstream phosphorylation and dimerization of IFN regulatory factor 3, resulting in the suppression of antiviral signaling and IFN induction in several cell types of human origin. Knockdown of NSs resulted in the suppression of SFTSV replication in host cells. Viral RNA bound to LSm14A-NSs protein complex during the interaction. A newly discovered motif of NSs functioned to interact with LSm14A. Altogether, our data demonstrated a mechanism used by SFTSV to inhibit host innate immune response.
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http://dx.doi.org/10.4049/jimmunol.2100148DOI Listing
July 2021

Causing Neonatal Meningitis During 2001-2020: A Study in Eastern China.

Int J Gen Med 2021 29;14:3007-3016. Epub 2021 Jun 29.

Department of Neonatology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People's Republic of China.

Background And Objective: Neonatal meningitis (NM) caused by remains a major health problem in industrialized countries. Currently, information on the epidemiology and antimicrobial susceptibility patterns of NM in developing countries such as China is relatively scarce. Therefore, the present study investigated changes in the antimicrobial susceptibility of causing NM in a perinatal center in eastern China over the past 20 years.

Methods: This survey was conducted during three periods: 2001-2006, 2007-2012, and 2013-2020. NM was diagnosed according to the number of white blood cells in the cerebrospinal fluid (CSF) and the presence of a single potential pathogenic bacterium in the culture prepared from the blood or CSF of a newborn baby. Changes in the antimicrobial susceptibility of were analyzed.

Results: In total, 182 NM cases were identified. was identified in 69 of these cases, and in 21 of these cases, extended-spectrum beta-lactamase (ESBL) production was detected. was the main cause of NM identified in this study. The overall susceptibility of to third-generation cephalosporins such as cefotaxime decreased from 100% during 2001-2006 to 50% during 2007-2012 and, subsequently, increased to 71.0% during 2013-2020. This pattern of change is correlated with bacterial ESBL production. Only 8.3% of found in samples collected from infants with early onset meningitis (EOM) produced ESBL, while 37.3% of isolated from children with late-onset meningitis (LOM) produced ESBL.

Conclusion: remains the primary pathogen of NM. Compared with that isolated from infants with LOM, the percentage of ESBL-producing multidrug-resistant isolated from infants with EOM is significantly lower. Clinicians should consider this trend when determining appropriate and effective antibiotics as empirical treatment for NM.
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http://dx.doi.org/10.2147/IJGM.S317299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254664PMC
June 2021

Stenting for pulmonary artery stenosis resistant to balloon pulmonary angioplasty in chronic thrombo-embolic pulmonary hypertension: a bail out strategy.

Eur Heart J Case Rep 2021 Mar 9;5(3):ytab071. Epub 2021 Mar 9.

Department of Nuclear Medicine, Gansu Provincial Hospital, No. 204, Donggang West Road, Chengguan District, Lanzhou 730000, Gansu, China.

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http://dx.doi.org/10.1093/ehjcr/ytab071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186910PMC
March 2021

Electrochemical Anion-Exchanged synthesis of porous Ni/Co hydroxide nanosheets for Ultrahigh-Capacitance supercapacitors.

J Colloid Interface Sci 2021 Oct 12;600:256-263. Epub 2021 May 12.

School of Materials Science and Engineering, Nanchang University, Nanchang 330031, China. Electronic address:

The commonly reported calcination strategy usually requires high temperature to crack the metal-organic frameworks (MOFs) particles, which often lead to uncontrollable growth of nanomaterials. Here, for the first time, we utilize an electrochemical anion-exchanged method to control the hydrolysis of MOFs and synthesize porous Ni/Co hydroxide nanosheets. After the electrochemical anion-exchange, the organic ligands of MOFs nanosheets can be recycled and reused. Applying an electric field to the MOFs bulk in alkaline solution can accelerate the nucleation rate of hydroxide and change the migration behavior of charged ions/molecules, which can tailor the microstructure of derivatives and improve deep charge and discharge capability of the electrodes. As a result, the hydroxide with the optimized Ni:Co molar ratio of 7:3 and electric-field application time of 1000 cycles [NiCo(OH)-1000c] provides much better electrochemical properties than the materials synthesized without electric-field assistance: a high specific capacitance of 2115C g (4230F g). A hybrid supercapacitor with the NiCo(OH)-1000c electrode shows a high energy density of 74.7 Wh kg, an improved power density (5,990.6 W kg), and an excellent cyclic stability (8,000 cycles). This study not only provides a novel strategy for the preparation of low-cost, deep-discharge electrodes for supercapacitors, but also proposes an unconventional method for mild synthesizing MOFs materials into porous nanoscale derivatives with tailored micromorphology.
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http://dx.doi.org/10.1016/j.jcis.2021.05.039DOI Listing
October 2021

Effects of HIF-1α on renal fibrosis in cisplatin-induced chronic kidney disease.

Clin Sci (Lond) 2021 05;135(10):1273-1288

Department of Nephrology, The Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Kidney Disease and Blood Purification, Hunan, China.

Cisplatin (Cis) can cause chronic kidney disease (CKD) and promote renal fibrosis, but the underlying mechanism is not fully understood. Hypoxia inducible factor-1α (HIF-1α) can promote renal fibrosis in some kidney diseases, but its role in Cis-induced CKD is still unknown. Notch-1 is a recognized molecule that promotes renal fibrosis under pathological circumstances, and evidence shows that HIF-1α and Notch-1 are closely related to each other. In the present study, mice with HIF-1α gene knockout in proximal tubular cells (PTCs) (PT-HIF-1α-KO) were generated and treated with Cis to induce CKD. A human proximal tubular cell line (HK-2) and primary mouse PTCs were used for in vitro studies. The results showed that HIF-1α was increased in the kidneys of Cis-treated wild-type mice, accompanied by elevated Notch-1, Notch-1 intracellular domain (N1ICD), Hes-1 and renal fibrosis. However, these alterations were partially reversed in PT-HIF-1α-KO mice. Similar results were observed in HK-2 cells and primary mouse PTCs. In addition, treating the cells with Cis induced a marked interaction of HIF-1α and N1ICD. Further inhibiting Notch-1 significantly reduced cellular fibrogenesis but did not affect HIF-1α expression. The data suggested that HIF-1α could promote renal fibrosis in Cis-induced CKD by activating Notch-1 both transcriptionally and post-transcriptionally and that HIF-1α may serve as a potential therapeutic target for Cis-induced CKD.
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http://dx.doi.org/10.1042/CS20210061DOI Listing
May 2021

DsbA-L Ameliorates Renal Injury Through the AMPK/NLRP3 Inflammasome Signaling Pathway in Diabetic Nephropathy.

Front Physiol 2021 30;12:659751. Epub 2021 Apr 30.

Department of Nephrology, Hunan Key Laboratory of Kidney Disease and Blood Purification, The Second Xiangya Hospital, Central South University, Changsha, China.

NLRP3-mediated inflammation is closely related to the pathological progression of diabetic nephropathy (DN). DsbA-L, an antioxidant enzyme, plays a protective role in a variety of diseases by inhibiting ER stress and regulating metabolism. However, the relationship of DsbA-L with inflammation, especially the NLRP3 inflammasome, has not been examined. In this study, we note that activation of the NLRP3 inflammasome and exacerbated fibrosis were observed in the kidneys of diabetic DsbA-L-knockout mice and were accompanied by decreased phosphorylation of AMP-activated protein kinase (AMPK). Moreover, correlation analysis shows that the phosphorylation of AMPK was negatively correlated with NLRP3 expression and tubular damage. In addition, the decreased AMPK phosphorylation and NLRP3 activation induced by high glucose (HG) in HK-2 cells could be alleviated by the overexpression of DsbA-L. Interestingly, the protective effect of DsbA-L was eliminated after treatment with compound C, a well-known AMPK inhibitor. Our findings suggest that DsbA-L inhibits NLRP3 inflammasome activation by promoting the phosphorylation of AMPK.
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http://dx.doi.org/10.3389/fphys.2021.659751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120163PMC
April 2021

Feature Extraction of Kidney Tissue Image Based on Ultrasound Image Segmentation.

J Healthc Eng 2021 26;2021:9915697. Epub 2021 Apr 26.

Department of Ultrasound, Harbin Medical University Fourth Hospital, Harbin 150001, Heilongjiang, China.

The kidney tissue image is affected by other interferences in the tissue, which makes it difficult to extract the kidney tissue image features, and it is difficult to judge the lesion characteristics and types by intelligent feature recognition. In order to improve the efficiency and accuracy of feature extraction of kidney tissue images, refer to the ultrasonic heart image for analysis and then apply it to the feature extraction of kidney tissue. This paper proposes a feature extraction method based on ultrasound image segmentation. Moreover, this study combines the optical flow method and the speckle tracking algorithm to select the best image tracking method and optimizes the algorithm speed through the full search method and the two-dimensional log search method. In addition, this study verifies the performance of the method proposed in this paper through comparative experimental research, and this study combines statistical analysis methods to perform data analysis. The research results show that the algorithm proposed in this paper has a certain effect.
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http://dx.doi.org/10.1155/2021/9915697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8093061PMC
April 2021

Infection of humanized mice with a novel phlebovirus presented pathogenic features of severe fever with thrombocytopenia syndrome.

PLoS Pathog 2021 05 11;17(5):e1009587. Epub 2021 May 11.

School of Life Sciences, Ningxia University, Yinchuan, P.R. China.

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne emerging phlebovirus with high mortality rates of 6.0 to 30%. SFTSV infection is characterized by high fever, thrombocytopenia, leukopenia, hemorrhage and multiple organ failures. Currently, specific therapies and vaccines remain elusive. Suitable small animal models are urgently needed to elucidate the pathogenesis and evaluate the potential drug and vaccine for SFTSV infection. Previous models presented only mild or no pathogenesis of SFTS, limiting their applications in SFTSV infection. Therefore, it is an urgent need to develop a small animal model for the investigation of SFTSV pathogenesis and evaluation of therapeutics. In the current report, we developed a SFTSV infection model based on the HuPBL-NCG mice that recapitulates many pathological characteristics of SFTSV infection in humans. Virus-induced histopathological changes were identified in spleen, lung, kidney, and liver. SFTSV was colocalized with macrophages in the spleen and liver, suggesting that the macrophages in the spleen and liver could be the principle target cells of SFTSV. In addition, histological analysis showed that the vascular endothelium integrity was severely disrupted upon viral infection along with depletion of platelets. In vitro cellular assays further revealed that SFTSV infection increased the vascular permeability of endothelial cells by promoting tyrosine phosphorylation and internalization of the adhesion molecule vascular endothelial (VE)-cadherin, a critical component of endothelial integrity. In addition, we found that both virus infection and pathogen-induced exuberant cytokine release dramatically contributed to the vascular endothelial injury. We elucidated the pathogenic mechanisms of hemorrhage syndrome and developed a humanized mouse model for SFTSV infection, which should be helpful for anti-SFTSV therapy and pathogenesis study.
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http://dx.doi.org/10.1371/journal.ppat.1009587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139491PMC
May 2021

Hepatocellular-cholangiocarcinoma with sarcomatous change: Clinicopathological features and outcomes.

Hepatobiliary Pancreat Dis Int 2021 Apr 17. Epub 2021 Apr 17.

Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yan-ta Road, Xi'an 710061, China; National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China. Electronic address:

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http://dx.doi.org/10.1016/j.hbpd.2021.03.011DOI Listing
April 2021

Extraction and detection of guanosine 5'-diphosphate-3'-diphosphate in amino acid starvation cells of Clavibacter michiganensis.

Braz J Microbiol 2021 Sep 10;52(3):1573-1580. Epub 2021 Apr 10.

Beijing Key Laboratory of Seed Disease Testing and Control, Department of Plant Pathology, China Agricultural University, Beijing, 100193, People's Republic of China.

Guanosine 5'-diphosphate-3'-diphosphate (ppGpp) is a small molecule nucleotide alarmone that can accumulate under the amino acid starvation state and trigger the stringent response. This study reported the extraction of ppGpp from the Gram-positive bacteria Clavibacter michiganensis through methods using formic acid, lysozyme, or methanol. Following extraction, ppGpp was detected through ultra-high-performance liquid chromatography (UHPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The methanol method showed the highest extraction efficiency for ppGpp among the three methods tested. C. michiganensis cells in exponential growth phase was induced in amino acid starvation by serine hydroxamate (SHX) and used for ppGpp extraction and detection. When using the methanol extraction method, the results showed that ppGpp concentrations in SHX-treated samples were 15.645 nM, 17.656 nM, 20.372 nM, and 19.280 nM at 0 min, 15 min, 30 min and 1 h, respectively, when detected using LC-MS/MS. This is the first report on ppGpp extraction and detection in Clavibacter providing a new idea and approach for nucleotide detection and extraction in bacteria.
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http://dx.doi.org/10.1007/s42770-021-00488-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8324763PMC
September 2021

HIF-1 Inhibitor YC-1 Reverses the Acquired Resistance of EGFR-Mutant HCC827 Cell Line with MET Amplification to Gefitinib.

Oxid Med Cell Longev 2021 3;2021:6633867. Epub 2021 Mar 3.

Department of Respiratory Medicine, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang 310012, China.

Background: Acquired resistance occurred in the majority of nonsmall cell lung cancer (NSCLC) patients receiving epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) therapy, and this may be related to the activation of the HIF-1 pathway. Therefore, we examined the influence of the hypoxia-inducible factor-1 (HIF-1) pathway inhibition on the sensitivity of HCC827 gefitinib-resistant (HCC827 GR) cells with MET amplification to gefitinib.

Methods: We established HCC827 GR cell line with MET amplification and set four groups with different treatment. An MTT assay, a colony formation analysis, and a wound healing assay were performed to determine the sensitivity change of HCC827 GR cells after different treatments. HIF-1, p-EGFR, and p-Met levels were detected with western blot. Correlations among HIF-1, p-EGFR, and p-Met levels of HCC827 GR cells with different treatments were analyzed with Pearson's correlation analysis.

Results: HIF-1 inhibitor YC-1 enhanced the sensitivity of HCC827 GR cells to gefitinib. p-Met level was correlated with HIF-1 level, while there was no correlation between p-Met level and p-EGFR level.

Conclusion: HIF-1 inhibitor YC-1 is able to reverse the acquired resistance of HCC827 GR to gefitinib, and the regulation of the HIF-1 pathway on MET may be one of the mechanisms.
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http://dx.doi.org/10.1155/2021/6633867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946473PMC
May 2021

MAMs Protect Against Ectopic Fat Deposition and Lipid-Related Kidney Damage in DN Patients.

Front Endocrinol (Lausanne) 2021 19;12:609580. Epub 2021 Feb 19.

Department of Nephrology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Kidney Disease and Blood Purification, Changsha, China.

Ectopic fat deposition (EFD) in the kidney plays a key role in the development of diabetic nephropathy (DN). Mitochondria-associated ER membranes (MAMs) are structures that connect to the endoplasmic reticulum (ER) and are involved in lipid metabolism. However, there are few studies on MAMs in the field of kidney disease, and the relationship between EFD and MAMs in DN is still unclear. In this study, increased EFD in the kidneys of DN patients was observed, and analysis showed that the degree of EFD was positively correlated with renal damage. Then, the MAMs were quantified by an proximity ligation assay (PLA). The MAMs in the kidneys were found to gradually decrease through the different stages of DN, while the expression of ADRP (a marker of lipid droplets) and tubulointerstitial damage increased. Moreover, correlation analysis showed that the MAMs were negatively correlated with serum lipid levels, the EFD in the kidney and renal damage. Finally, we observed decreased expression of MAM-control proteins (DsbA-L, PACS-2, and MFN-2) in different stages of DN and they were associated with lipid deposition and renal damage. These data showed that the destruction of MAMs in DN might be the cause of EFD and interstitial damage in the kidney.
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http://dx.doi.org/10.3389/fendo.2021.609580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7933555PMC
February 2021

αKlotho protein has therapeutic activity in contrast-induced acute kidney injury by limiting NLRP3 inflammasome-mediated pyroptosis and promoting autophagy.

Pharmacol Res 2021 05 4;167:105531. Epub 2021 Mar 4.

Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Contrast-induced acute kidney injury (CI-AKI) is a main cause of hospital-acquired renal failure. Nevertheless, limited measures have been shown to be effective for the treatment of CI-AKI. Here, we demonstrated that αKlotho, which is highly expressed in kidney, has therapeutic activity in CI-AKI. Our data showed that αKlotho expression levels were decreased both in the kidney and serum of CI-AKI mice. Administration of αKlotho protein protected the kidney and HK-2 cells against contrast-induced injury. Mechanistically, αKlotho reduced contrast-induced renal tubular cells pyroptosis by limiting NLRP3 inflammasome activation. Meanwhile, αKlotho up-regulated autophagy via inhibiting the AKT/mTOR pathway and decreased mitochondrial ROS level. Inhibition of autophagy blunted the suppression effect of αKlotho on NLRP3 inflammasome activation and cell pyroptosis in contrast-treated HK-2 cells. Taken together, our data suggest that αKlotho protein protects against CI-AKI through inhibiting NLRP3 inflammasome-mediated pyroptosis, which is likely by promoting autophagy. αKlotho may be a promising therapeutic strategy for CI-AKI.
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http://dx.doi.org/10.1016/j.phrs.2021.105531DOI Listing
May 2021

Evaluation of percutaneous transcatheter embolization for pulmonary arteriovenous malformations.

BMC Pulm Med 2021 Mar 5;21(1):77. Epub 2021 Mar 5.

Division of Cardiovascular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Background: The purpose of this study was to assess the safety and efficacy of percutaneous transcatheter embolization (TCE) for the treatment of pulmonary arteriovenous malformations (PAVMs).

Methods: Forty-three consecutive patients (n = 17 males; n = 26 females) with 72 untreated PAVMs underwent coil and/or plug embolization between January 2010 and February 2018. The mean patient age was 42 ± 14 years (range 19-71 years). The median size of the feeding artery was 7.9 ± 2.9 mm (range 3.5-14.0 mm). The arterial blood gas level and cardiac function of all patients were analysed. The technical success rate, recanalization rate, and complications were evaluated. Computed tomography angiography (CTA) examinations were scheduled for 12 months after treatment and every 2-4 years thereafter.

Results: Twenty-five PAVMs were treated with coils alone, twenty-one were treated with plugs alone, and twenty-six were treated with both coils and plugs. The technical success rate was 100%. There were no complications during operation. However, one patient (2.3%) had pulmonary thrombosis and embolism post-operation. The patients' pre-operative and post-operative PaO and SaO levels were significantly different (p < 0.01). A comparison of the New York Heart Association (NYHA) grade before and after embolization in all patients showed a significant decrease in the post-operative grade (p < 0.01). The 72 PAVMs were divided into three groups (coils only group [n = 25], plugs only group [n = 21], and coils/plugs combined group [n = 26]). After 12 months of follow-up, there were seven reperfusion PAVMs in the coil group, seven reperfusion PAVMs in the plug group, and 1 reperfusion PAVM in the combined group. There were significant differences between the two groups and the combined group.

Conclusion: Percutaneous TCE is safe and effective for the treatment of PAVMs. A combination of coils and vascular plugs may be useful for preventing recanalization after the embolization of PAVMs.
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http://dx.doi.org/10.1186/s12890-021-01448-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934521PMC
March 2021

Diagnostic value and prognostic significance of CTRP9 combined with pentraxin-3 in acute coronary syndrome.

Exp Ther Med 2021 Mar 25;21(3):254. Epub 2021 Jan 25.

Department of Cardiology, Dongying Hospital of Traditional Chinese Medicine, Dongying, Shandong 257000, P.R. China.

The present study aimed to explore the diagnostic value and prognostic significance of C1q/tumor necrosis factor-related protein 9 (CTRP9) combined with pentraxin-3 (PTX-3) in acute coronary syndrome (ACS). A total of 137 patients with coronary heart disease and chest pain were included. Among them, seventy-nine patients with ACS were allocated into a study group and fifty-eight patients with non-cardiac chest pain (NCCP) were allocated into a control group. The serum CTRP9, PTX-3 levels were quantified by ELISA, and their correlation with other ACS-related indexes, diagnostic value for ACS and predictive significance for poor prognosis were analyzed. In addition, the risk factors of the poor prognosis of ACS patients were studied. CTRP9 was lowly expressed and PTX-3 was highly expressed in the serum of ACS patients. CTRP9 was negatively correlated with cardiac troponin I (cTnI), creatine kinase-MB (CK-MB) and high-sensitivity C-reactive protein (hs-CRP) (P<0.05), while PTX-3 was positively correlated with them (P<0.05). Combined detection of CTRP9 and PTX-3 was of high value in the diagnosis and prognosis of ACS patients. In addition, CTRP9 and PTX-3 were independent risk factors for the poor prognosis of ACS. Patients with ACS had lower CTRP9 expression and higher PTX-3 expression than those without ACS. Moreover, the combined detection of CTRP9 and PTX-3 can better evaluate the diagnosis and prognosis of ACS patients.
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http://dx.doi.org/10.3892/etm.2021.9685DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851676PMC
March 2021

CCR5 signaling promotes lipopolysaccharide-induced macrophage recruitment and alveolar developmental arrest.

Cell Death Dis 2021 02 15;12(2):184. Epub 2021 Feb 15.

Department of Neonatology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kong Jiang Road, 200092, Shanghai, China.

The pathogenesis of bronchopulmonary dysplasia (BPD), involves inflammatory, mechanisms that are not fully characterized. Here we report that overexpression of C-C chemokine receptor 5 (CCR5) and its ligands is associated with BPD development. Lipopolysaccharide-induced BPD rats have increased CCR5 and interleukin-1β (IL-1β) levels, and decreased alveolarization, while CCR5 or IL-1β receptor antagonist treatments decreased inflammation and increased alveolarization. CCR5 enhances macrophage migration, macrophage infiltration in the lungs, IL-1β levels, lysyl oxidase activity, and alveolar development arrest. CCR5 expression on monocytes, and its ligands in blood samples from BPD infants, are elevated. Furthermore, batyl alcohol supplementation reduced CCR5 expression and IL-1β production in lipopolysaccharide-exposed rat lungs. Moreover, receptor-interacting kinase 3 (RIP3) upstream regulator of CCR5-cultured RIP3 macrophages exhibited partly blocked lipopolysaccharide-induced CCR5 expression. We conclude that increased CCR5 expression is a key mechanism in BPD development and represents a novel therapeutic target for treatment.
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http://dx.doi.org/10.1038/s41419-021-03464-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883330PMC
February 2021

Progress in understanding the role of lncRNA in programmed cell death.

Cell Death Discov 2021 Feb 8;7(1):30. Epub 2021 Feb 8.

Jiangxi Research Institute of Ophthalmology and Visual Sciences, Affiliated Eye Hospital of Nanchang University, Nanchang, 330006, China.

Long non-coding RNAs (lncRNAs) are transcripts longer than 200 nucleotides but not translated into proteins. LncRNAs regulate gene expressions at multiple levels, such as chromatin, transcription, and post-transcription. Further, lncRNAs participate in various biological processes such as cell differentiation, cell cycle regulation, and maintenance of stem cell pluripotency. We have previously reported that lncRNAs are closely related to programmed cell death (PCD), which includes apoptosis, autophagy, necroptosis, and ferroptosis. Overexpression of lncRNA can suppress the extrinsic apoptosis pathway by downregulating of membrane receptors and protect tumor cells by inhibiting the expression of necroptosis-related proteins. Some lncRNAs can also act as competitive endogenous RNA to prevent oxidation, thereby inhibiting ferroptosis, while some are known to activate autophagy. The relationship between lncRNA and PCD has promising implications in clinical research, and reports have highlighted this relationship in various cancers such as non-small cell lung cancer and gastric cancer. This review systematically summarizes the advances in the understanding of the molecular mechanisms through which lncRNAs impact PCD.
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http://dx.doi.org/10.1038/s41420-021-00407-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870930PMC
February 2021

Competitive microbial degradation among PBDE congeners in anaerobic wetland sediments: Implication by multiple-line evidences including compound-specific stable isotope analysis.

J Hazard Mater 2021 06 25;412:125233. Epub 2021 Jan 25.

Navigation College, Dalian Maritime University, Dalian 116026, China.

Polybrominated diphenyl ethers (PBDEs) are widespread contaminants in the environment. Microbial reductive debromination is one of the important attenuation processes for PBDEs in the anaerobic sediments. This study first investigated the interaction between BDE-47 and BDE-153 during the microbial degradation in wetland sediments by the multiple-line approaches including biodegradation kinetics, microbial community structures and stable isotope composition. BDE-47 and BDE-153 biodegradation fitted pseudo-zero-order kinetics, with the higher degradation rates in single than combined exposure, indicating the mutual inhibition in co-exposure condition. BDE-47 and BDE-153 shared the common dehalogenators (genus Dehalococcoides and Acinetobacter) with enrichment in combined exposure, indicating the potential competition in dehalogenating bacteria during biodegradation. Microbial degradation could lead to the isotopic fractionation of BDE-47 and BDE-153, with the smaller changes in δC in combined than single exposure. The apparent kinetic isotope effect of carbon (AKIE) was different between BDE-47 and BDE-153 in single exposure, whilst identical in combined exposure, indicating the similar degradation mechanism for BDE-47 and BDE-153 in co-exposure condition. These results revealed that the competition on microbial degradation occurred among PBDEs in co-exposure condition, which was important for the comprehensive risk assessment of simultaneous exposure to multiple PBDE congeners in the environment.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125233DOI Listing
June 2021

Tailoring P2/P3 Biphases of Layered Na MnO by Co Substitution for High-Performance Sodium-Ion Battery.

Small 2021 Feb 27;17(7):e2007103. Epub 2021 Jan 27.

Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), School of Materials Science and Engineering, Shandong University, Jingshi Road 17923, Jinan, 250061, P. R. China.

P-type layered oxide is a promising cathode candidate for sodium-ion batteries (SIBs), but faces the challenge of simultaneously realizing high rate capability and long cycle life. Herein, Co-substituted Na MnO nanosheets with tunable P2/P3 biphase structures are synthesized by a novel dealloying-annealing strategy. The optimized P2/P3-Na Mn Co Al O cathode delivers an excellent rate capability of 83 mA h g at a high current density of 1700 mA g (10 C), and an outstanding cycling stability over 500 cycles at 1000 mA g . This excellent performance is attributed to the unique P2/P3 biphases with stable crystal structures and fast Na diffusion between open prismatic Na sites. Moreover, operando X-ray diffraction is applied to explore the structural evolution of Na Mn Co Al O during the Na extraction/insertion processes, and the P2-P2' phase transition is effectively suppressed. Operando Raman technique is utilized to explore the structural superiority of P2/P3 biphase cathode compared with pure P2 or P3 phase. This work highlights precisely tailoring the phase composition as an effective strategy to design advanced cathode materials for SIBs.
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http://dx.doi.org/10.1002/smll.202007103DOI Listing
February 2021

First isolation and pathogenicity analysis of a genogroup U strain of infectious hematopoietic necrosis virus from rainbow trout in China.

Transbound Emerg Dis 2021 Jan 8. Epub 2021 Jan 8.

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.

Infectious hematopoietic necrosis virus (IHNV) is a major fish viral pathogen causing acute clinical disease and death in a variety of salmonids. IHNV isolates have been classified into five major genogroups according to the phylogenetic analysis of partial G gene fragments or the complete G gene sequence: U, M, E, L and J. Genogroup U strains have been reported in North America and Japan prior to 1982, and genogroup J is the only genogroup that has been reported in China. Here, one of IHNV strain (BjLL) was isolated from a local farm in China and were characterized in this study. The homogenate tissues of infected fry induced IHNV-positive cytopathic effects in epithelioma papulosum cyprinid (EPC) cells that were confirmed by RT-PCR and sequencing. The complete genome sequence of BjLL comprised 11,129 nucleotides, which had been submitted to GenBank (accession no. MF509592). By the sequence comparison and phylogenetic analysis for the G gene sequence of BjLL with 51 reference sequences in GenBank, we confirmed that this Chinese isolate belonged to genogroup U. Furthermore, virus exposure experiments with juvenile rainbow trout were conducted to assess the virulence and pathogenicity of BjLL. Compared with GS-2014 of genogroup J, BjLL was an obviously less virulent strain that could result in lower mortality. Besides, typical clinical symptoms and pathological damages could be seen in fish following infection of BjLL. The present study is the first report of genogroup U IHNV infection in China and will provide essential information for future studies on pathogenesis of IHNV BjLL and development of efficient control strategies.
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http://dx.doi.org/10.1111/tbed.13983DOI Listing
January 2021

Role of Penicillin-Binding Proteins in the Viability, Morphology, Stress Tolerance, and Pathogenicity of .

Phytopathology 2021 Aug 11;111(8):1301-1312. Epub 2021 Sep 11.

Department of Plant Pathology, College of Plant Protection, China Agricultural University; Beijing Key Laboratory of Seed Disease Testing and Control, Beijing 100193, P.R. China.

Previous research has shown that penicillin-binding proteins (PBPs), enzymes involved in peptidoglycan (PG) assembly, could play an important role during the induction of the viable but nonculturable (VBNC) state, which allows non-spore-forming bacteria to survive adverse environmental conditions. The current study found that has seven PBPs. Mutant analysis indicated that deletion of either of the class B PBPs was lethal and that the class A PBPs had an important role in PG synthesis, with the Δ mutant having an altered cellular morphology that resulted in longer cells that were swollen at one end and had thinner cell walls. The mutant was also found to produce mucoid colonies in solid culture and a lower final cell titer in liquid medium, as well as having high sensitivity to osmotic stress and lysozyme treatment and surprisingly high pathogenicity. The double mutant, , also had a slightly altered phenotype, resulting in longer cells. Further analysis revealed that both mutants had high sensitivity to copper, which resulted in quicker induction into the VBNC state. However, only the mutant had significantly reduced survivorship in the VBNC state. The study also confirmed that the VBNC state significantly improved the survivorship of wild-type cells in response to environmental stresses and systemically demonstrated the protective role of the VBNC state in , which is an important finding regarding its epidemiology and has serious implications for disease management.
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http://dx.doi.org/10.1094/PHYTO-08-20-0326-RDOI Listing
August 2021
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