Publications by authors named "Na Han"

457 Publications

HER2 exon 20 insertion mutations in lung adenocarcinoma with leptomeningeal metastasis: a case report and response to poziotinib.

Ann Palliat Med 2021 May 31. Epub 2021 May 31.

Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (lung and esophagus), Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, China; Department of Thoracic Medical Oncology, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Hangzhou, China; Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China; The First Clinical Medical College, Wenzhou Medical University, Wenzhou, China.

Leptomeningeal metastasis (LM) is associated with poor prognosis and represents a terminal event of non-small cell lung cancers (NSCLC). In previous studies, most of LM-patients have detected epidermal growth factor receptor (EGFR) mutation and responded to the third generation of EGFR-tyrosine kinase inhibitor (TKI). This study aimed to report a case of ERBB2 (HER2) exon 20 insertion mutations in the cerebrospinal fluid (CSF) of LM-patient which response to poziotinib. At the beginning, postoperative pathology showed a primary invasive adenocarcinoma with no mutations in EGFR and ROS-1. Pemetrexed plus carboplatin combined with bevacizumab was administered as the first-line followed by bevacizumab alone for continuation maintenance therapy. Targeted therapy and immunotherapy were given after the disease progressed in two months. Subsequently, the patient developed mental symptoms and adenocarcinoma cells were found in the CSF. Next-generation sequencing (NGS) results showed HER2 exon 20 insertion mutations in the primary tissue, CSF and plasma samples. Then, poziotinib was administered and the symptoms improved significantly after 3 days and the progress free survival was nearly 2 months. Therefore, we speculate that the CSF concentration and penetration rate of poziotinib may significantly higher than of other TKIs so that it achieves a higher CSF concentration than standard dosing, and successfully controlled LM. It may provide a new therapeutic option for LM-patient and may be especially who are lung adenocarcinoma with HER2 exon 20 insertion.
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http://dx.doi.org/10.21037/apm-21-213DOI Listing
May 2021

Average Approximate Hashing-Based Double Projections Learning for Cross-Modal Retrieval.

IEEE Trans Cybern 2021 Jun 9;PP. Epub 2021 Jun 9.

Cross-modal retrieval has attracted considerable attention for searching in large-scale multimedia databases because of its efficiency and effectiveness. As a powerful tool of data analysis, matrix factorization is commonly used to learn hash codes for cross-modal retrieval, but there are still many shortcomings. First, most of these methods only focus on preserving locality of data but they ignore other factors such as preserving reconstruction residual of data during matrix factorization. Second, the energy loss of data is not considered when the data of cross-modal are projected into a common semantic space. Third, the data of cross-modal are directly projected into a unified semantic space which is not reasonable since the data from different modalities have different properties. This article proposes a novel method called average approximate hashing (AAH) to address these problems by: 1) integrating the locality and residual preservation into a graph embedding framework by using the label information; 2) projecting data from different modalities into different semantic spaces and then making the two spaces approximate to each other so that a unified hash code can be obtained; and 3) introducing a principal component analysis (PCA)-like projection matrix into the graph embedding framework to guarantee that the projected data can preserve the main energy of data. AAH obtains the final hash codes by using an average approximate strategy, that is, using the mean of projected data of different modalities as the hash codes. Experiments on standard databases show that the proposed AAH outperforms several state-of-the-art cross-modal hashing methods.
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http://dx.doi.org/10.1109/TCYB.2021.3081615DOI Listing
June 2021

Exploring the mediating role of serum retinol-binding protein 4 in the relationship between sleep quality and insulin resistance in pregnant women.

Diabetes Res Clin Pract 2021 May 21;176:108866. Epub 2021 May 21.

Department of Maternal and Child Health, School of Public Health, Peking University, Beijing 100191, China.

Aims: We aimed to explore the mediating role of plasma retinol-binding protein 4 (RBP4) in the relationship between sleep quality and insulin resistance (IR) among pregnant women.

Methods: We conducted a cross-sectional study including 263 pregnant women in the first trimester. Sleep quality was evaluated by Pittsburgh Sleep Quality Index (PSQI). The ELISA and homeostasis model assessment (HOMA) was used to analyze plasma RBP4 and estimate IR. The mediating model was used to analyze the mediating role of RBP4 in the relationship between PSQI score and IR.

Results: In the multivariable linear regression model, the three terms were positively related with each other, PSQI score was positively associated with IR levels (β = 0.55, p < 0.05). In the mediating model, RBP4 levels mediated completely the relationship between PSQI scores and IR levels (β = 0.29, p < 0.0001). The indirect effect of RBP4 in the relation between sleep quality and IR explained 89.10% of total effect.

Conclusions: RPB4 may play a complete mediating role in the relation between sleep quality and insulin resistance in early pregnancy. Improvements in sleep quality in the first trimester may provide a pathway to reduce plasma RBP4, which is beneficial for less IR and GDM prevention.
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http://dx.doi.org/10.1016/j.diabres.2021.108866DOI Listing
May 2021

High expression of PDGFA predicts poor prognosis of esophageal squamous cell carcinoma.

Medicine (Baltimore) 2021 May;100(20):e25932

Department of Physiology and Neurobiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, PR China.

Abstract: Platelet-derived growth factor A (PDGFA), the most known member of PDGF family, plays a crucial role in occurrence and progression of different tumors. However, PDGFA expression and its clinical significance in esophageal squamous cell carcinoma (ESCC) are not clear. The present study aimed to assess the expression and prognostic value of PDGFA in ESCC.The Gene Expression Omnibus databases (GSE53625, GSE23400, and GSE67269) and fresh clinical samples were employed for detecting PDGFA messenger RNA expression in ESCC. The associations of PDGFA expression with clinicopathological characteristics were evaluated by chi-square test. Kaplan-Meier analysis and Cox proportional hazard regression model were performed to determine the prognostic value of PDGFA in ESCC patients. PDGFA-related signaling pathways were defined by gene set enrichment analysis based on Gene Expression Omnibus databases.The PDGFA messenger RNA expression was upregulated in ESCC tissues compared with paired adjacent noncancerous tissues (P < .05) and was positively correlated with T stage (P < .05). Kaplan-Meier survival analysis suggested that ESCC patients with high PDGFA expression were associated with poorer overall survival compared to those with low PDGFA expression (P < .05), especially in advanced T stage (P < .05). Cox analyses showed that high expression of PDGFA was an independent predictor for poor prognosis in ESCC patients. Gene set enrichment analysis identified 3 signaling pathways (extracellular matrix receptor interaction, focal adhesion, and glycosaminoglycan biosynthesis chondroitin sulfate) that were enriched in PDGFA high expression phenotype (all P < .01).PDGFA may serve as an oncogene in ESCC and represent an independent molecular biomarker for prognosis of ESCC patients.
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http://dx.doi.org/10.1097/MD.0000000000025932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137088PMC
May 2021

Acceptance of a COVID-19 vaccine and associated factors among pregnant women in China: a multi-center cross-sectional study based on health belief model.

Hum Vaccin Immunother 2021 May 14:1-10. Epub 2021 May 14.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China.

: Vaccine hesitancy has been recognized as an urgent public health issue. We aimed to explore the acceptance of a COVID-19 vaccine and related factors among pregnant women, a vulnerable population for vaccine-preventable diseases. A multi-center cross-sectional study among pregnant women was conducted in five provinces of mainland China from November 13 to 27, 2020. We collected sociodemographic characteristics, attitude, knowledge, and health beliefs on COVID-19 vaccination. Locally weighted scatterplot smoothing regression analysis was used to assess the trends of vaccination acceptance. Multivariable logistic regression was performed to identify factors related to vaccination acceptance. Among the 1392 pregnant women, the acceptance rate of a COVID-19 vaccine were 77.4% (95%CI 75.1-79.5%). In the multivariable regression model, the acceptance rate was associated with young age (aOR = 1.87, 95% CI: 1.20-2.93), western region (aOR = 2.73, 95% CI: 1.72-4.32), low level of education (aOR = 2.49, 95% CI: 1.13-5.51), late pregnancy (aOR = 1.49, 95% CI: 1.03-2.16), high knowledge score on COVID-19 (aOR = 1.05, 95% CI: 1.01-1.10), high level of perceived susceptibility (aOR = 2.18, 95% CI: 1.36-3.49), low level of perceived barriers (aOR = 4.76, 95% CI: 2.23-10.18), high level of perceived benefit (aOR = 2.18, 95% CI: 1.36-3.49), and high level of perceived cues to action (aOR = 15.70, 95% CI: 8.28-29.80). About one quarters of pregnant women have vaccine hesitancy. Our findings highlight that targeted and multipronged efforts are needed to build vaccine literacy and confidence to increase the acceptance of a COVID-19 vaccine during the COVID-19 pandemic, especially for vulnerable populations.
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http://dx.doi.org/10.1080/21645515.2021.1892432DOI Listing
May 2021

Size-Dependent Selectivity of Electrochemical CO Reduction on Converted In O Nanocrystals.

Angew Chem Int Ed Engl 2021 May 11. Epub 2021 May 11.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, 215123, China.

The size modulation of catalyst particles represents a useful dimension to tune catalytic performances by impacting not only their surface areas but also local electronic structures. It, however, has remained inadequately explored and poorly elucidated. Here, we report the interesting size-dependent selectivity of electrochemical CO reduction on In O nanocrystals. 5-nm nanoparticles and 15-nm nanocubes with focused size distribution are prepared via a facile solvothermal reaction in oleylamine by carefully controlling a set of experimental parameters. They serve as the precatalysts, and are reduced to In nanocrystals while largely inherit the original size feature during electrochemical CO reduction. Catalyst derived from 15-nm nanocubes exhibits greater formate selectivity (>95 %) at lower overpotential and negligible side reactions compared to bulk-like samples (indium foil and 200-nm cubes) as well as the catalyst derived from smaller 5-nm nanoparticles. This unique size dependence is rationalized as a result of the competition among different reaction pathways by our theoretical computations. Smaller is not always better in the catalyst design.
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http://dx.doi.org/10.1002/anie.202105256DOI Listing
May 2021

Convergent evolution of SARS-CoV-2 in human and animals.

Protein Cell 2021 Apr 30. Epub 2021 Apr 30.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, AMMS, Beijing, 100071, China.

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http://dx.doi.org/10.1007/s13238-021-00847-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085470PMC
April 2021

The Positive Effect of ZnS in Waste Tire Carbon as Anode for Lithium-Ion Batteries.

Materials (Basel) 2021 Apr 24;14(9). Epub 2021 Apr 24.

Centre for Clean Environment and Energy, Gold Coast Campus, Griffith University, Gold Coast, QLD 4222, Australia.

There is great demand for high-performance, low-cost electrode materials for anodes of lithium-ion batteries (LIBs). Herein, we report the recovery of carbon materials by treating waste tire rubber via a facile one-step carbonization process. Electrochemical studies revealed that the waste tire carbon anode had a higher reversible capacity than that of commercial graphite and shows the positive effect of ZnS in the waste tire carbon. When used as the anode for LIBs, waste tire carbon shows a high specific capacity of 510.6 mAh·g at 100 mA·g with almost 97% capacity retention after 100 cycles. Even at a high rate of 1 A·g, the carbon electrode presents an excellent cyclic capability of 255.1 mAh·g after 3000 cycles. This high-performance carbon material has many potential applications in LIBs and provide an alternative avenue for the recycling of waste tires.
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http://dx.doi.org/10.3390/ma14092178DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122980PMC
April 2021

Two-Dimensional Palladium-Copper Alloy Nanodendrites for Highly Stable and Selective Electrochemical Formate Production.

Nano Lett 2021 May 21;21(9):4092-4098. Epub 2021 Apr 21.

Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou 215123, China.

Pd is the only metal that can catalyze electrochemical CO reduction to formate at close-to-zero overpotential. It is unfortunately subjected to severe poisoning by trace CO as the side product and suffers from deteriorating stability and selectivity with increasing overpotential. Here, we demonstrate that alloying Pd with Cu in the form of two-dimensional nanodendrites could enable highly stable and selective formate production. Such unique bimetallic nanostructures are formed as a result of the rapid in-plane growth and suppressed out-of-plane growth by carefully controlling a set of experimental parameters. Thanks to the combined electronic effect and nanostructuring effect, our alloy product catalyzes CO reduction to formate with remarkable stability and selectivity under the working potential as cathodic as -0.4 V. Our results are rationalized by computational simulations, evidencing that Cu atoms weaken the *CO adsorption and stabilize the *OCHO adsorption on neighboring Pd atoms.
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http://dx.doi.org/10.1021/acs.nanolett.1c01113DOI Listing
May 2021

Flexible Carbon Nanotubes Confined Yolk-Shelled Silicon-Based Anode with Superior Conductivity for Lithium Storage.

Nanomaterials (Basel) 2021 Mar 11;11(3). Epub 2021 Mar 11.

Key Laboratory of Materials Physics, and Anhui Key Laboratory of Nanomaterials and Nanotechnology, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei 230031, China.

The further deployment of silicon-based anode materials is hindered by their poor rate and cycling abilities due to the inferior electrical conductivity and large volumetric changes. Herein, we report a silicon/carbon nanotube (Si/CNT) composite made of an externally grown flexible carbon nanotube (CNT) network to confine inner multiple Silicon (Si) nanoparticles (Si NPs). The in situ generated outer CNTs networks, not only accommodate the large volume changes of inside Si NPs but also to provide fast electronic/ionic diffusion pathways, resulting in a significantly improved cycling stability and rate performance. This Si/CNT composite demonstrated outstanding cycling performance, with 912.8 mAh g maintained after 100 cycles at 100 mA g, and excellent rate ability of 650 mAh g at 1 A g after 1000 cycles. Furthermore, the facial and scalable preparation method created in this work will make this new Si-based anode material promising for practical application in the next generation Li-ion batteries.
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http://dx.doi.org/10.3390/nano11030699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8001621PMC
March 2021

The association of prenatal exposure to particulate matter with infant growth: A birth cohort study in Beijing, China.

Environ Pollut 2021 May 3;277:116792. Epub 2021 Mar 3.

Department of Epidemiology and Preventive Medicine, School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia.

Limited studies examined the associations of prenatal exposure to particulate matter (PM) and children's growth with inconsistent results, and no study focused on PM. We matched a birth cohort (10,547 children) with daily PM and PM concentrations by maternal home addresses. Air pollution concentrations were predicted by satellite remote sensing data, meteorological factors, and land use information. The weight and length of children in the birth cohort were measured at approximately one year old. We calculated the Z-score of weight for length (WFL) and body mass index (BMI) and then defined overweight and obesity (OWOB) based on WHO Standards. Generalized linear regression and modified Poisson regression were used to identify the association of prenatal exposure to PM or PM with anthropometric measurements and risk of OWOB. We also determined the mediation effect of preterm birth on the associations. Results showed that a 10 μg/m increase in prenatal exposure to PM and PM was significantly associated with a 0.105 [95% confidence interval (CI): 0.067, 0.144] and 0.063 (95% CI: 0.029, 0.097) increase in WFL Z-score for one-year-old children. Similar associations were found for BMI Z-score. A 10 μg/m increase in prenatal PM and PM exposure was significantly associated with 1.012 (95%CI: 1.003, 1.021) and 1.010 (95%CI: 1.002, 1.018) times higher risk of OWOB. . Preterm birth mediated 7.5% [direct effect (DE) = 0.106, P < 0.001; indirect effect (IE) = 0.009, P < 0.001)] and 9.9% (DE = 0.064, P < 0.001; IE = 0.007, P < 0.001) of the association between prenatal PM and PM exposure and WFL Z-score of the children. The association of prenatal PM and PM exposure with BMI Z-score of children was also mediated by preterm birth by 6.6% (DE = 0.111, P < 0.001; IE = 0.008, P < 0.001) and 9.1% (DE = 0.064, P < 0.001; IE = 0.006, P < 0.001). These results remained robust in the sensitivity analyses. In conclusion, prenatal exposure to PM and PM increased WFL, BMI Z-scores and higher risk of OWOB for one-year-old children. The associations were partially mediated by preterm birth. These findings call for the urgent action on air pollution regulation to protect early-life health among children.
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http://dx.doi.org/10.1016/j.envpol.2021.116792DOI Listing
May 2021

Anticoagulation and Transjugular Intrahepatic Portosystemic Shunt for the Management of Portal Vein Thrombosis in Cirrhosis: A Prospective Observational Study.

Am J Gastroenterol 2021 Feb 25. Epub 2021 Feb 25.

Department of Liver Diseases and Digestive Interventional Radiology, National Clinical Research Centre for Digestive Diseases and Xi'an International Medical Center Hospital of Digestive Diseases, Northwestern University, Xi'an, China; Department of Liver Diseases and Interventional Radiology, Xi'an International Medical Center Hospital of Digestive Diseases, Northwestern University, Xi'an, China; Department of Ultrasound, National Clinical Research Centre for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China; State Key Laboratory of Cancer Biology, National Clinical Research Centre for Digestive Diseases and Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, China.

Introduction: Current guidelines recommend anticoagulation as the mainstay of portal vein thrombosis (PVT) treatment in cirrhosis. However, because of the heterogeneity of PVT, anticoagulation alone does not always achieve satisfactory results. This study aimed to prospectively evaluate an individualized management algorithm using a wait-and-see strategy (i.e., no treatment), anticoagulation, and transjugular intrahepatic portosystemic shunt (TIPS) to treat PVT in cirrhosis.

Methods: Between February 2014 and June 2018, 396 consecutive patients with cirrhosis with nonmalignant PVT were prospectively included in a tertiary care center, of which 48 patients (12.1%) were untreated, 63 patients (15.9%) underwent anticoagulation, 88 patients (22.2%) underwent TIPS, and 197 patients (49.8%) received TIPS plus post-TIPS anticoagulation. The decision of treatment option mainly depends on the stage of liver disease (symptomatic portal hypertension or not) and degree and extension of thrombus.

Results: During a median 31.7 months of follow-up period, 312 patients (81.3%) achieved partial (n = 25) or complete (n = 287) recanalization, with 9 (3.1%) having rethrombosis, 64 patients (16.2%) developed major bleeding (anticoagulation-related bleeding in 7 [1.8%]), 88 patients (22.2%) developed overt hepatic encephalopathy, and 100 patients (25.3%) died. In multivariate competing risk regression models, TIPS and anticoagulation were associated with a higher probability of recanalization. Long-term anticoagulation using enoxaparin or rivaroxaban rather than warfarin was associated with a decreased risk of rethrombosis and an improved survival, without increasing the risk of bleeding. However, the presence of complete superior mesenteric vein thrombosis was associated with a lower recanalization rate, increased risk of major bleeding, and poor prognosis.

Discussion: In patients with cirrhosis with PVT, the individualized treatment algorithm achieves a high-probability recanalization, with low rates of portal hypertensive complications and adverse events.
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http://dx.doi.org/10.14309/ajg.0000000000001194DOI Listing
February 2021

Association between the COVID-19 pandemic and the risk for adverse pregnancy outcomes: a cohort study.

BMJ Open 2021 02 23;11(2):e047900. Epub 2021 Feb 23.

Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China

Objectives: The secondary impacts of the COVID-19 pandemic on adverse maternal and neonatal outcomes remain unclear. In this study, we aimed to evaluate the association between the COVID-19 pandemic and the risk for adverse pregnancy outcomes.

Design: We conduced retrospective analyses on two cohorts comprising 7699 pregnant women in Beijing, China, and compared pregnancy outcomes between the pre-COVID-2019 cohort (women who delivered from 20 May 2019 to 30 November 2019) and the COVID-2019 cohort (women who delivered from 20 January 2020 to 31 July 2020). The secondary impacts of the COVID-2019 pandemic on pregnancy outcomes were assessed by using multivariate log-binomial regression models, and we used interrupted time-series (ITS) regression analysis to further control the effects of time-trends.

Setting: One tertiary-level centre in Beijing, China PARTICIPANTS: 7699 pregnant women.

Results: Compared with women in the pre-COVID-19 pandemic group, pregnant women during the COVID-2019 pandemic were more likely to be of advanced age, exhibit insufficient or excessive gestational weight gain and show a family history of chronic disease (all p0.05). After controlling for other confounding factors, the risk of premature rupture of membranes and foetal distress was increased by 11% (95% CI, 1.04 to 1.18; p<0.01) and 14% (95% CI, 1.01 to 1.29; p<0.05), respectively, during the COVID-2019 pandemic. The association still remained in the ITS analysis after additionally controlling for time-trends (all p<0.01). We uncovered no other associations between the COVID-19 pandemic and other pregnancy outcomes (p>0.05).

Conclusions: During the COVID-19 pandemic, more women manifested either insufficient or excessive gestational weight gain; and the risk of premature rupture of membranes and foetal distress was also higher during the pandemic.
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http://dx.doi.org/10.1136/bmjopen-2020-047900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7907630PMC
February 2021

Antioxidant and -Glucosidase Inhibitory Activities Guided Isolation and Identification of Components from Mango Seed Kernel.

Oxid Med Cell Longev 2020 28;2020:8858578. Epub 2020 Dec 28.

Department of Pharmacognosy and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China.

In the present study, petroleum ether, dichloromethane, ethyl acetate, and -butanol fractions of mango seed kernel exhibited different degrees of antioxidant and -glucosidase inhibitory activity. Thus, quantitative and qualitative analysis of the petroleum ether fraction was conducted by GC-MS. Among identified components, four unsaturated fatty acids had never been reported in natural products before, together with 19 known components. In addition, 17 compounds were isolated and elucidated from other active fractions. Compounds , , , and were isolated for the first time from genus. Compounds and exhibited prominent DPPH radical scavenging and -glucosidase inhibitory effects. In order to further explore their mechanism of -glucosidase inhibition, their enzyme kinetics and in silico modeling experiments were performed. The results indicated that inhibited -glucosidase in a noncompetitive manner, whereas acted in a competitive manner. In molecular docking, the stability of binding was enhanced by - T-shaped, -alkyl, - stacked, hydrogen bond, and electrostatic interactions. Thus, compounds and were determined to be new potent antioxidant and -glucosidase inhibitors for preventing food oxidation and enhancing hypoglycemic activity.
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http://dx.doi.org/10.1155/2020/8858578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785352PMC
December 2020

Maternal sleep quality during early pregnancy, risk factors and its impact on pregnancy outcomes: a prospective cohort study.

Sleep Med 2021 03 2;79:11-18. Epub 2021 Jan 2.

Department of Maternal and Child Health, School of Public Health, Peking University, Beijing, China.

Objectives: We aimed to assess the prevalence of poor sleep quality during early pregnancy and its risk factors, and to explore the association between sleep quality and adverse pregnancy outcomes.

Methods: This was a prospective birth cohort study that included 4352 pregnant women. Sleep quality were assessed using the Pittsburgh Sleep Quality Index (PSQI). The risk factors for poor sleep quality were analyzed by a logistic regression model. Log-binomial regression models were used to analyze the association between sleep quality and pregnancy outcomes.

Results: The prevalence of maternal poor sleep quality during early pregnancy was 34.14%. The multivariate logistic model showed that stillbirth history (OR = 2.45; 95% CI: 1.34, 4.47), history of induced abortion (OR = 1.26; 95% CI: 1.07, 1.49), general health-related quality of life (OR = 3.98; 95% CI: 2.97, 5.34), insufficient physical activity (OR = 1.18; 95% CI: 1.03, 1.36), smoking (OR = 1.59; 95% CI: 1.18, 2.15), and vegetarian (OR = 2.18; 95% CI: 1.54, 3.08) were risk factors for poor sleep quality, while taking folic acid consistently before pregnancy (OR = 0.83; 95% CI: 0.72,0.97) was the protective factor. After controlling for all the confounders, poor sleep quality during early pregnancy increased the risk of premature rupture of membranes by 12% (95% CI: 1.00, 1.25).

Conclusion: Pregnant women with a history of stillbirth and induced abortion, general health-related quality of life, insufficient physical activity, smoking, and a vegetarian diet tended to have poor sleep quality. More attention should be paid to healthy lifestyle of pregnant women to improve sleep quality and better pregnancy outcomes.
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http://dx.doi.org/10.1016/j.sleep.2020.12.040DOI Listing
March 2021

Prenatal exposure to airborne particulate matter of 1 μm or less and fetal growth: A birth cohort study in Beijing, China.

Environ Res 2021 03 9;194:110729. Epub 2021 Jan 9.

Department of Maternal and Child Health, School of Public Health, Peking University, National Health Commission Key Laboratory of Reproductive Health, Beijing, China. Electronic address:

Background: The association of airborne particulate matter of 1 μm or less (PM) with fetal growth hasn't been studied. We aimed to investigate the association of PM with fetal growth parameters measured via ultrasonography and birth weight.

Methods: The birth cohort included 18,669 pregnant women who were pregnant between 2014 and 2017 in Tongzhou Maternal and Child Health Hospital of Beijing, China. The predicted PM concentration was matched with the residential addresses of each woman. The fetal abdominal circumference (AC), head circumference (HC), femur length (FL) and estimated fetal weight (EFW) were evaluated via ultrasonography, while birth weight was measured at birth. The fetal parameters and birth weight were standardized as gestational-age- and gender-adjusted Z-score. We defined undergrowth of fetal parameters, low birth weight (LBW) and small-for-gestational-age (SGA) as categorized outcomes. Generalized estimating equations and generalized linear regression were used to examine the associations of PM with quantitative and categorized outcomes, respectively.

Results: A 10 μg/m increase in PM was associated with decrement in the Z-scores of AC [-0.027, 95% confidence intervals (CI): -0.047~ -0.07]EFW (-0.055, 95%CI: -0.075~-0.035). These results remained robust after adjusting nitrogen dioxide and sulphur dioxide. We didn't observe significant results regarding the analyses of undergrowth of all fetal parameters and the analyses of birth weight outcomes.

Conclusion: This study identified the negative associations between PM and fetal parameters in utero. The findings provided robust evidence that strategies for reducing PM exposure can prevent early-life health.
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http://dx.doi.org/10.1016/j.envres.2021.110729DOI Listing
March 2021

Melatonin alleviates d-galactose-decreased hyaluronic acid production in synovial membrane cells via Sirt1 signalling.

Cell Biochem Funct 2021 Jun 11;39(4):488-495. Epub 2021 Jan 11.

Department of Clinical Medicine, Logistics University of People's Armed Police Force, Tianjin, China.

Hyaluronic acid (HA) exerts a critical role in the lubricating and buffering properties of synovial fluid in joints. The production of HA is regulated by growth factors, hormones, inflammatory cytokines and mechanical load. The reduction of HA contributes to the progression of osteoarthritis. Herein, we found that d-galactose (d-gal) induced the senescence of rabbit synovial membrane cells, accompanied by decreased HA production. The mRNA level of HA synthase 2 (HAS2) was downregulated by d-gal, as analysed by real-time polymerase chain reaction. Melatonin, an endocrine hormone, can regulate the homeostasis of bone and cartilage. We found that melatonin treatment attenuated d-gal-induced cell senescence and decreased the expression of p21, p16 and pp65 proteins. Melatonin could reverse HA production and maintain HAS2 expression. Furthermore, we revealed that Sirt1 signalling was required for melatonin effects. Sirt1 inhibitor could counteract melatonin-mediated HA production and HAS2 expression. Additionally, Sirt1 overexpression directly antagonized d-gal-induced cell aging and HA downregulation. Taken together, our results suggest that melatonin-Sirt1 signal has a protective effect on synovial membrane cells, enhancing HA synthesis and interrupting cell senescence.
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http://dx.doi.org/10.1002/cbf.3613DOI Listing
June 2021

Reply to "Can femoral head necrosis induced by steroid therapy in patients infected with coronaviruses be reversed?"

Bone Res 2021 Jan 7;9(1). Epub 2021 Jan 7.

Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, China.

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http://dx.doi.org/10.1038/s41413-020-00133-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788274PMC
January 2021

Total cholesterol concentration predicts the effect of plasmapheresis on hypertriglyceridemic acute pancreatitis: a retrospective case-control study.

BMC Gastroenterol 2021 Jan 6;21(1). Epub 2021 Jan 6.

Intensive Care Unit, The First Affiliated Hospital of Xiamen University, NO. 55, Zhenhai Road, Siming District, Xiamen City, Fujian, 361003, People's Republic of China.

Background: What kind of patients with hypertriglyceridemic acute pancreatitis (HLAP) might benefit from plasmapheresis (PP) remains unknown. The objective of this study is to determine the predict function of total cholesterol (TC) on the Triglyceride (TG)-lowing effect in patients on either non-PP or PP therapy.

Methods: Patients were categorized into high total cholesterol (HTC)/low total cholesterol (LTC) groups based on TC level of 12.4 mmol/L. The primary outcome was TG reduction to below 500 mg/dL within 48 h. Linear mixed-effect model and logistic regression analyses were used to assess the association of TC level and TG-lowing efficacy in different therapy groups.

Results: Compared with LTC group, patients with HTC showed more severe imaging manifestations (p < 0.001) and higher APACH II scores (p = 0.036). Deaths occurred only in HTC groups. Significant interaction of time sequence with the 2 TGs-lowing therapy groups on TG level was only found in HTC group (p < 0.001). In patients with elevated TC level, primary outcome occurred in 66.67% of patients in the PP group, and 27.91% in the non-PP group. After adjustment for age, gender, CT grade and APACH II score, the odd ratio remain significant (OR 5.47, 95% confidence interval [CI] 1.84-16.25, p = 0.002). Furthermore, in patients with lower TC level, no significant difference was found in primary outcome between PP group and non-PP group (81.25% versus 62.30%, adjusted OR 2.05; 95% CI 0.45-9.40; p = 0.353).

Conclusions: TC could be a potential biomarker to predict the effects of TG-lowing therapy in patients with HLAP.
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http://dx.doi.org/10.1186/s12876-020-01572-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7789165PMC
January 2021

[A new quantitative 16S rRNA amplicon sequencing method].

Sheng Wu Gong Cheng Xue Bao 2020 Dec;36(12):2548-2555

Department of Bioinformatics, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

In recent years, 16S rRNA amplicon sequencing technology has been widely used to study human gut microbiota and to detect unknown pathogens in clinical samples. However, its resolution to bacterial population can only reach the relative abundance of genus level, and different factors affect the final bacterial profile, such as sample concentrations, PCR cycle numbers and amplification primers. In order to solve these problems, we developed a quantitative 16S rRNA amplicon sequencing method by combining random tag and internal marker method. The new methods improved the accuracy of human gut microbiota, reduced the impact of experimental operation on the results, and improved the comparability between sequencing and other molecular biological methods.
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http://dx.doi.org/10.13345/j.cjb.200353DOI Listing
December 2020

Association between sleep duration in early pregnancy and risk of gestational diabetes mellitus: a prospective cohort study.

Diabetes Metab 2020 Dec 16;47(5):101217. Epub 2020 Dec 16.

Department of Maternal and Child Health, School of Public Health, Peking University, Beijing, China; Key Laboratory of Reproductive Health, National Health and Family Planning Commission of the People's Republic of China, Beijing, China.

Aims: As cohort studies of the impact of sleep duration during early pregnancy on gestational diabetes mellitus (GDM) are lacking, our study aimed to explore the association between sleep duration in the first trimester and GDM in one region of mainland China.

Methods: For this prospective cohort study, sleep duration data were collected from 3692 pregnant women at the first prenatal care appointment before 14 weeks of gestation. Multivariable log-binomial regression models were used to analyze the association of sleep duration with GDM after adjusting for demographic characteristics, health status (such as family history of diabetes, history of GDM, prepregnancy body mass index, gestational weight gain) and lifestyle habits (such as physical activity, dietary intakes).

Results: Our cohort included 166 (4.5%) short sleepers and 505 (14%) long sleepers. Shorter sleep duration was more likely to be observed in women aged ≥35 years who were multiparous, and had previous pregnancy, insufficient gestational weight gain, engaged in more vigorous physical activity, drank alcohol, were vegan and/or never took folic-acid supplements. Compared with normal sleepers (29%), the prevalence of GDM was significantly higher in short sleepers (38%; P = 0.01), but not in long sleepers (31%; P = 0.224). In the multivariable model, women with short sleep durations during early pregnancy had a 32% greater risk of GDM [adjusted risk ratio (aRR): 1.32, 95% CI: 1.06-1.63], whereas long sleepers did not (aRR: 1.09, 95% CI: 0.94-1.26).

Conclusion: Short sleep duration during early pregnancy is associated with an increased risk of GDM. This suggests that more attention should be paid to controlling the development of GDM in pregnant women with insufficient sleep.
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http://dx.doi.org/10.1016/j.diabet.2020.101217DOI Listing
December 2020

Comparative viromes of Culicoides and mosquitoes reveal their consistency and diversity in viral profiles.

Brief Bioinform 2020 Dec 14. Epub 2020 Dec 14.

Suzhou Institute of Systems Medicine.

The genus Culicoides includes biting midges, some of which are vectors for viruses that cause diseases in humans and animals. Knowledge of the roles of Culicoides in viral ecology is inadequate. We collected ~300 000 samples of Culicoides and mosquitoes in 15 representative regions within Yunnan, China. Using mosquitoes as reference vectors, we designed a comparative virome strategy to study the viral composition, diversity, hosts and spatiotemporal distribution of Culicoides. A map of viromes in Culicoides and mosquitoes in Yunan province, China, was constructed. At the same locations, Culicoides and mosquitoes usually share a similar viral diversity. At least 10 important pathogenic viruses were detected from Culicoides. Many novel viruses were discovered, including 21 segmented viruses of Flaviviridae, 180 viruses of Monjiviricetes and 130 viruses of Bunyavirales. The findings demonstrate that Culicoides is an important part of viral ecology and should be studied and monitored for potentially emerging viruses.
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http://dx.doi.org/10.1093/bib/bbaa323DOI Listing
December 2020

Social Media Use, Unhealthy Lifestyles, and the Risk of Miscarriage Among Pregnant Women During the COVID-19 Pandemic: Prospective Observational Study.

JMIR Public Health Surveill 2021 01 5;7(1):e25241. Epub 2021 Jan 5.

Department of Obstetrics and Gynecology, Maternal and Child Health Hospital of Tongzhou District, Beijing, China.

Background: The COVID-19 pandemic has resulted in changes to normal life and disrupted social and economic function worldwide. However, little is known about the impact of social media use, unhealthy lifestyles, and the risk of miscarriage among pregnant women during the COVID-19 pandemic.

Objective: This study aims to assess the association between social media use, unhealthy lifestyles, and the risk of miscarriage among pregnant women in the early stage of the COVID-19 pandemic in China.

Methods: In this prospective cohort study, 456 singleton pregnant women in mainland China were recruited during January and February 2020. Sociodemographic characteristics, history of previous health, social media use, and current lifestyles were collected at baseline, and we followed up about the occurrence of miscarriage. Log-binomial regression models were used to estimate the risk ratios (RRs) of miscarriage for women with different exposures to COVID-19-specific information.

Results: Among all the 456 pregnant women, there were 82 (18.0%) who did no physical activities, 82 (18.0%) with inadequate dietary diversity, 174 (38.2%) with poor sleep quality, and 54 (11.8%) spending >3 hours on reading COVID-19 news per day. Women with excessive media use (>3 hours) were more likely to be previously pregnant (P=.03), have no physical activity (P=.003), have inadequate dietary diversity (P=.03), and have poor sleep quality (P<.001). The prevalence of miscarriage was 16.0% (n=73; 95% CI 12.6%-19.4%). Compared with women who spent 0.5-2 hours (25/247, 10.1%) on reading COVID-19 news per day, miscarriage prevalence in women who spent <0.5 hours (5/23, 21.7%), 2-3 hours (26/132, 19.7%), and >3 hours (17/54, 31.5%) was higher (P<.001). Miscarriage prevalence was also higher in pregnant women with poor sleep quality (39/174, 22.4% vs 34/282, 12.1%; P=.003) and a high education level (66/368, 17.9% vs 7/88, 8.0%; P=.02). In the multivariable model, poor sleep quality (adjusted RR 2.06, 95% CI 1.24-3.44; P=.006), 2-3 hours of media use daily (adjusted RR 1.74, 95% CI 1.02-2.97; P=.04), and >3 hours of media use daily (adjusted RR 2.56, 95% CI 1.43-4.59; P=.002) were associated with miscarriage. In the sensitivity analysis, results were still stable.

Conclusions: Pregnant women with excessive media use were more likely to have no physical activity, inadequate dietary diversity, and poor sleep quality. Excessive media use and poor sleep quality were associated with a higher risk of miscarriage. Our findings highlight the importance of healthy lifestyles during the COVID-19 pandemic.
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http://dx.doi.org/10.2196/25241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787689PMC
January 2021

Alloyed Palladium-Silver Nanowires Enabling Ultrastable Carbon Dioxide Reduction to Formate.

Adv Mater 2021 Jan 4;33(4):e2005821. Epub 2020 Dec 4.

Institute of Functional Nano and Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials and Devices, Soochow University, Suzhou, 215123, China.

Palladium can enable the electrochemical CO reduction to formate with nearly zero overpotential and good selectivity. However, it usually has very limited stability owing to CO poisoning from the side reaction intermediate. Herein, it is demonstrated that alloying palladium with silver is a viable strategy to significantly enhance the electrocatalytic stability. Palladium-silver alloy nanowires are prepared in aqueous solution with tunable chemical compositions, large aspect ratio, and roughened surfaces. Thanks to the unique synergy between palladium and silver, these nanowires exhibit outstanding electrocatalytic performances for selective formate production. Most remarkably, impressive long-term stability is measured even at < -0.4 V versus reversible hydrogen electrode where people previously believed that formate cannot be stably formed on palladium. Such stability results from the enhanced CO tolerance and selective stabilization of key reaction intermediates on alloy nanowires as supported by detailed electrochemical characterizations and theoretical computations.
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http://dx.doi.org/10.1002/adma.202005821DOI Listing
January 2021

Identification of four differentially expressed genes associated with acute and chronic spinal cord injury based on bioinformatics data.

Neural Regen Res 2021 May;16(5):865-870

Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education; Department of Trauma and Orthopedics, Peking University People's Hospital, Beijing, China.

Complex pathological changes occur during the development of spinal cord injury (SCI), and determining the underlying molecular events that occur during SCI is necessary for the development of promising molecular targets and therapeutic strategies. This study was designed to explore differentially expressed genes (DEGs) associated with the acute and chronic stages of SCI using bioinformatics analysis. Gene expression profiles (GSE45006, GSE93249, and GSE45550) were downloaded from the Gene Expression Omnibus database. SCI-associated DEGs from rat samples were identified, and Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed. In addition, a protein-protein interaction network was constructed. Approximately 66 DEGs were identified in GSE45550 between 3-14 days after SCI, whereas 2418 DEGs were identified in GSE45006 1-56 days after SCI. Moreover, 1263, 195, and 75 overlapping DEGs were identified between these two expression profiles, 3, 7/8, and 14 days after SCI, respectively. Additionally, 16 overlapping DEGs were obtained in GSE45006 1-14 days after SCI, including Pank1, Hn1, Tmem150c, Rgd1309676, Lpl, Mdh1, Nnt, Loc100912219, Large1, Baiap2, Slc24a2, Fundc2, Mrps14, Slc16a7, Obfc1, and Alpk3. Importantly, 3882 overlapping DEGs were identified in GSE93249 1-6 months after SCI, including 3316 protein-coding genes and 567 long non-coding RNA genes. A comparative analysis between GSE93249 and GSE45006 resulted in the enrichment of 1135 overlapping DEGs. The significant functions of these 1135 genes were correlated with the response to the immune effector process, the innate immune response, and cytokine production. Moreover, the biological processes and KEGG pathways of the overlapping DEGs were significantly enriched in immune system-related pathways, osteoclast differentiation, the nuclear factor-κB signaling pathway, and the chemokine signaling pathway. Finally, an analysis of the overlapping DEGs associated with both acute and chronic SCI, assessed using the expression profiles GSE93249 and GSE45006, identified four overlapping DEGs: Slc16a7, Alpk3, Lpl and Nnt. These findings may be useful for revealing the biological processes associated with SCI and the development of targeted intervention strategies.
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http://dx.doi.org/10.4103/1673-5374.297087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178775PMC
May 2021

Experimental study of superheating of tin powders.

Sci Rep 2020 Nov 4;10(1):19026. Epub 2020 Nov 4.

Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, Republic of Korea.

An unstable energy-unbalanced state such as superheating or supercooling is often unexpectedly observed because a factor of energy depends not only on the temperature but is a product of temperature (T) and entropy (S). Thus, at the same temperature, if the entropy is different, the total energy of the system can be different. In such cases, the temperature-change-rate cannot match the entropy-change-rate, which results in a hysteresis curve for the temperature/entropy relationship. Due to the difference between the temperature- and entropy-change-rates, properties of a material, such as the boiling and freezing points, can be extended from point to area. This study confirmed that depending on the heating rate, tin powders exhibit different melting points. Given the contemporary reinterpretation of many energy-non-equilibrium phenomena that have only been discussed on the basis of temperature, this study is expected to contribute to the actual expansion of scientific/engineering applications.
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http://dx.doi.org/10.1038/s41598-020-76223-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643175PMC
November 2020

miR-375-3p contributes to hypoxia-induced apoptosis by targeting forkhead box P1 (FOXP1) and Bcl2 like protein 2 (Bcl2l2) in rat cardiomyocyte h9c2 cells.

Biotechnol Lett 2021 Feb 30;43(2):353-367. Epub 2020 Oct 30.

Department of Cardiology, Qingdao Central Hospital, No. 127, Siliu South Road, Qingdao, 266042, Shandong, China.

miRNAs have been pointed to play critical role in the development of congenital heart disease (CHD). miRNA-375-3p (miR-375-3p) was involved in cardiac dysfunction and cardiogenesis. However, no prior study had established a therapeutic role of miR-375-3p in CHD. We intended to investigate the effect and mechanism of miR-375-3p on apoptosis in hypoxic cardiomyocytes in vitro. Expression of miR-375-3p, forkhead box P1 (FOXP1) and Bcl2 like protein 2 (Bcl2l2) was detected using real-time quantitative PCR and western blot. Apoptosis was measured with MTT assay, flow cytometry and caspase-3 activity assay. The potential target binding between miR-375-3p and FOXP1/Bcl2l2 was predicted on DianaTools, and was validated by luciferase reporter assay and RNA pull-down assay. As a result, miR-375-3p was upregulated and FOXP1/Bcl2l2 was downregulated in maternal serum of women with fetal CHD and hypoxia-induced rat cardiomyocyte h9c2 cells. Hypoxia induced apoptosis rate elevation, caspase-3 activity promotion and viability inhibition in h9c2 cells; overexpression of miR-375-3p promoted, whereas knockdown of miR-375-3p antagonized hypoxia-induced effects in h9c2 cells. In addition, miR-375-3p was validated to negatively regulate FOXP1 and Bcl2l2 expression through target binding, and silencing of FOXP1 and Bcl2l2 could independently abate the anti-apoptosis role of miR-375-3p knockdown in hypoxic h9c2 cells. Collectively, blocking miR-375-3p suppressed hypoxia-evoked apoptosis of cardiomyocytes by targeting and upregulating FOXP1 and Bcl2l2. Our results might suggest maternal serum miR-375-3p as a potential biomarker for prenatal detection of fetal CHD.
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http://dx.doi.org/10.1007/s10529-020-03013-wDOI Listing
February 2021

Compositional diversity and evolutionary pattern of coronavirus accessory proteins.

Brief Bioinform 2021 03;22(2):1267-1278

Suzhou Institute of Systems Medicine, Center for Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Jiangsu, Suzhou, China.

Accessory proteins play important roles in the interaction between coronaviruses and their hosts. Accordingly, a comprehensive study of the compositional diversity and evolutionary patterns of accessory proteins is critical to understanding the host adaptation and epidemic variation of coronaviruses. Here, we developed a standardized genome annotation tool for coronavirus (CoroAnnoter) by combining open reading frame prediction, transcription regulatory sequence recognition and homologous alignment. Using CoroAnnoter, we annotated 39 representative coronavirus strains to form a compositional profile for all of the accessary proteins. Large variations were observed in the number of accessory proteins of 1-10 for different coronaviruses, with SARS-CoV-2 and SARS-CoV having the most (9 and 10, respectively). The variation between SARS-CoV and SARS-CoV-2 accessory proteins could be traced back to related coronaviruses in other hosts. The genomic distribution of accessory proteins had significant intra-genus conservation and inter-genus diversity and could be grouped into 1, 4, 2 and 1 types for alpha-, beta-, gamma-, and delta-coronaviruses, respectively. Evolutionary analysis suggested that accessory proteins are more conservative locating before the N-terminal of proteins E and M (E-M), while they are more diverse after these proteins. Furthermore, comparison of virus-host interaction networks of SARS-CoV-2 and SARS-CoV accessory proteins showed that they share multiple antiviral signaling pathways, those involved in the apoptotic process, viral life cycle and response to oxidative stress. In summary, our study provides a tool for coronavirus genome annotation and builds a comprehensive profile for coronavirus accessory proteins covering their composition, classification, evolutionary pattern and host interaction.
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http://dx.doi.org/10.1093/bib/bbaa262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665327PMC
March 2021

MDACP: A Pathogen Genome and Metagenome Analysis Cloud Platform.

Front Genet 2020 31;11:1007. Epub 2020 Aug 31.

State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China.

Pathogenic microorganism analysis based on next-generation sequencing technology is an important tool for clinical diagnosis, public health surveillance, and outbreak investigation. However, scientific researchers without the relevant background lack the time, training, or infrastructure to use large data sets or install and use command line tools. Therefore, the bioinformatic team at the Chinese Center for Disease Control and Prevention developed the Microbial Data Analysis Cloud Platform (MDACP) as a safe, professional, and efficient pathogen genetic data analysis platform for rapid microbial data mining, such as for candidate pathogen detection, genome typing, and traceability. MDACP is a web service system based on the Docker platform and can be used for data analysis on various operating systems. The platform focuses on pathogen analysis and continuously develops new analysis processes according to the analysis needs of the users. This platform has a friendly user interface and is easy to operate, allowing users to submit data through data pages or graphical clients, flexibly control parameters according to data conditions, and analyze data in parallel with multiple tasks. Researchers can quickly carry out bioinformatic analyses without coding work, promote follow-up research and information mining of projects, and improve the utilization of big data in the field of disease control. MDACP enables research personnel to conduct data analysis and management and assists clinicians and disease control personnel with mining information, such as pathogen identification, classification, and traceability.
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http://dx.doi.org/10.3389/fgene.2020.01007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489492PMC
August 2020

Investigation of Age-Related Changes in the Skin Microbiota of Korean Women.

Microorganisms 2020 Oct 14;8(10). Epub 2020 Oct 14.

KustoGen Inc., Chuncheon 24341, Korea.

The microbiota of human skin is influenced by host and environmental factors. To determine if chronological age influences the composition of the skin microbiota on the forehead and hands, 73 Korean women were sorted into one of three age groups: (1) 10-29 years ( = 24), (2) 30-49 years ( = 21), and (3) 50-79 years ( = 28). From the 73 women, 146 skin samples (two skin sites per person) were collected. 16S rRNA gene amplicon sequencing was then conducted to analyze the skin microbiota. The overall microbial distribution varied on the forehead but was similar on the hands across the three age groups. In addition, the composition of the skin microbiota differed between the forehead and hands. Commensal microbiota, such as Streptococcus, Staphylococcus, Cutibacterium, and Corynebacterium, which contribute to maintaining skin health via dominant occupation, were affected by increasing age on forehead and hand skin. Alpha diversity indices increased significantly with age on forehead skin. This study indicates that older people may be more susceptible to pathogenic invasions due to an imbalanced skin microbiota resulting from age-related changes. The results of our study may help develop new strategies to rebalance skin microbiota shifted during aging.
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http://dx.doi.org/10.3390/microorganisms8101581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7602415PMC
October 2020