Publications by authors named "Na Fan"

55 Publications

mRNA-based therapeutics: powerful and versatile tools to combat diseases.

Signal Transduct Target Ther 2022 05 21;7(1):166. Epub 2022 May 21.

Department of Critical Care Medicine, Frontiers Science Center for Disease-related Molecular Network, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.

The therapeutic use of messenger RNA (mRNA) has fueled great hope to combat a wide range of incurable diseases. Recent rapid advances in biotechnology and molecular medicine have enabled the production of almost any functional protein/peptide in the human body by introducing mRNA as a vaccine or therapeutic agent. This represents a rising precision medicine field with great promise for preventing and treating many intractable or genetic diseases. In addition, in vitro transcribed mRNA has achieved programmed production, which is more effective, faster in design and production, as well as more flexible and cost-effective than conventional approaches that may offer. Based on these extraordinary advantages, mRNA vaccines have the characteristics of the swiftest response to large-scale outbreaks of infectious diseases, such as the currently devastating pandemic COVID-19. It has always been the scientists' desire to improve the stability, immunogenicity, translation efficiency, and delivery system to achieve efficient and safe delivery of mRNA. Excitingly, these scientific dreams have gradually been realized with the rapid, amazing achievements of molecular biology, RNA technology, vaccinology, and nanotechnology. In this review, we comprehensively describe mRNA-based therapeutics, including their principles, manufacture, application, effects, and shortcomings. We also highlight the importance of mRNA optimization and delivery systems in successful mRNA therapeutics and discuss the key challenges and opportunities in developing these tools into powerful and versatile tools to combat many genetic, infectious, cancer, and other refractory diseases.
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http://dx.doi.org/10.1038/s41392-022-01007-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123296PMC
May 2022

A Novel Electrocardiography Model for the Diagnosis of Acute Pulmonary Embolism.

Front Cardiovasc Med 2022 11;9:825561. Epub 2022 Apr 11.

Sichuan Provincial People's Hospital, Chengdu, China.

Acute pulmonary embolism (acPE) is a severe disease that is often misdiagnosed as it is difficult to detect quickly and accurately. In this study, a novel electrocardiogram (ECG) model was used to estimate the probability of acPE rapidly analysis of ECG characteristics. A total of 327 patients with acPE who were diagnosed at the Sichuan Provincial People's Hospital (SPPH) between 2018 and 2021 were retrospectively studied. A total of 331 patients were randomly selected as the control group, which included patients hospitalized during the same time period. The control group included patients who presented with characteristic symptoms of acPE, but this diagnosis was ruled out following further diagnostic testing. This study compared the diagnostic value of the ECG model with those of another ECG scoring model (Daniel-ECG score) and the most common prediction models (Wells score and Geneva score). This study established an ECG-predictive model using analysis of the ECG abnormalities in patients with acPE. The final ECG model included certain novel ECG signs that had not been incorporated in the previous ECG score of the patients, and thus, compared to the previous ECG score, exhibited a more favorable area under the receiver operating characteristic curve (AUC) value (0.8741). The model developed in this study was named the SPPH-ECG model. Furthermore, this study compared the SPPH-ECG model with Daniel-ECG score, Wells score, and Geneva score, and the SPPH-ECG model was demonstrated to exhibit a superior AUC value (0.8741), sensitivity (79.08%), negative predictive value (79.52%), and test accuracy (79.42%), while the Geneva score presented superior specificity (100%) and positive predictive value (100%) compared with the SPPH-ECG model. In conclusion, the SPPH-ECG model may play a role in ruling out acPE in patients during diagnostic testing and diagnose acPE rapidly and accurately in combination with the Geneva scoring system.
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http://dx.doi.org/10.3389/fcvm.2022.825561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9035687PMC
April 2022

Reproductive and developmental toxicities of 5-fluorouracil in model organisms and humans.

Expert Rev Mol Med 2022 01 31;24:e9. Epub 2022 Jan 31.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China.

Chemotherapy, as an important clinical treatment, has greatly enhanced survival in cancer patients, but the side effects and long-term sequelae bother both patients and clinicians. 5-Fluorouracil (5-FU) has been widely used as a chemotherapeutic agent in the clinical treatment of various cancers, but several studies showed its adverse effects on reproduction. Reproductive toxicity of 5-FU often associates with developmental block, malformation and ovarian damage in the females. In males, 5-FU administration alters the morphology of sexual organs, the levels of reproductive endocrine hormones and the progression of spermatogenesis, ultimately reducing sperm numbers. Mechanistically, 5-FU exerts its effect through incorporating the active metabolites into nucleic acids directly, or inhibiting thymidylate synthase to disrupt the function of DNA and RNA, leading to profound effects on cellular metabolism and viability. However, some studies suggested that the toxicity of 5-FU on reproduction is reversible and certain drugs used in combination with 5-FU during chemotherapy could protect reproductive systems from 5-FU damage both in females and males. Herein, we summarise the recent findings and discuss underlying mechanisms of the 5-FU-induced reproductive toxicity, providing a reference for future research and clinical treatments.
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http://dx.doi.org/10.1017/erm.2022.3DOI Listing
January 2022

Effects of konjac glucomannan/pomegranate peel extract composite coating on the quality and nutritional properties of fresh-cut kiwifruit and green bell pepper.

J Food Sci Technol 2022 Jan 26;59(1):228-238. Epub 2021 Feb 26.

College of Healthy Management, Shangluo University, Shangluo, 726000 People's Republic of China.

The effects of an edible coating, based on konjac glucomannan (KG) incorporated with pomegranate peel extracts (PE), on the physicochemical and nutritional properties of fresh-cut kiwifruit and green bell pepper during storage were investigated. The optimal extract time (40.6 min), temperature (54.5 °C), and ultrasound power (255.5 W) with response surface method, provided a high total antioxidant activity (TAA) of (92.31 ± 1.43)%. Fresh-cut kiwifruit and green bell pepper were coated by dipping using five treatments (distilled water, ascorbic acid, KG, PE, KG + PE), packed into polymeric film and stored for 8 days at 10 °C. Distilled water treatment was used as control. KG + PE treatment resulted in the highest total soluble solid and titratable acidity in fresh-cut kiwifruit, while the maximum firmness in fresh-cut green bell pepper. The weight loss was both effectively decreased in samples treated with KG or KG + PE. All samples treated with KG + PE had significantly higher contents of chlorophyll, ascorbic acid, total phenolic and TAA than others. Moreover, the KG + PE group had the lowest counts of microorganisms in all samples. KG coating incorporated with PE was proved to be efficient in maintaining the physico-chemical and nutritional properties of fresh-cut kiwifruit and green bell pepper during low temperature storage compared with control.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-021-05006-7.
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http://dx.doi.org/10.1007/s13197-021-05006-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758865PMC
January 2022

Reply to Charrel, R.N.; Depaquit, J. Comment on "Xu et al. Isolation and Identification of a Novel Phlebovirus, Hedi Virus, from Sandflies Collected in China. 2021, , 772".

Viruses 2021 12 3;13(12). Epub 2021 Dec 3.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.

Dear Professor Remi N. Charrel and Professor Jerome Depaquit, we thank you for your interest in our research and for your kind suggestions [...].
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http://dx.doi.org/10.3390/v13122422DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8704664PMC
December 2021

Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.

Epigenetics Chromatin 2021 12 14;14(1):55. Epub 2021 Dec 14.

State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, 24 Zhaojun Road, Yuquan District, Hohhot, 010070, Inner Mongolia, China.

Background: Asf1 is a well-conserved histone chaperone that regulates multiple cellular processes in different species. Two paralogous genes, Asf1a and Asf1b exist in mammals, but their role during fertilization and early embryogenesis remains to be investigated further.

Methods: We analyzed the dynamics of histone chaperone Asf1a and Asf1b in oocytes and pre-implantation embryos in mice by immunofluorescence and real-time quantitative PCR, and further investigated the role of Asf1a and Asf1b during fertilization and pre-implantation development by specific Morpholino oligos-mediated knock down approach.

Results: Immunofluorescence with specific antibodies revealed that both Asf1a and Asf1b were deposited in the nuclei of fully grown oocytes, accumulated abundantly in zygote and 2-cell embryonic nuclei, but turned low at 4-cell stage embryos. In contrast to the weak but definite nuclear deposition of Asf1a, Asf1b disappeared from embryonic nuclei at morula and blastocyst stages. The knockdown of Asf1a and Asf1b by specific Morpholino oligos revealed that Asf1a but not Asf1b was required for the histone H3.3 assembly in paternal pronucleus. However, knockdown of either Asf1a or Asf1b expression decreased developmental potential of pre-implantation embryos. Furthermore, while Asf1a KD severely reduced H3K56 acetylation level and the expression of Oct4 in blastocyst stage embryos, Asf1b KD almost eliminated nuclear accumulation of proliferating cell marker-PCNA in morula stage embryos. These results suggested that histone chaperone Asf1a and Asf1b play distinct roles during fertilization and pre-implantation development in mice.

Conclusions: Our data suggested that both Asf1a and Asf1b are required for pre-implantation embryonic development. Asf1a regulates H3K56ac levels and Oct4 expression, while Asf1b safeguards pre-implantation embryo development by regulating cell proliferation. We also showed that Asf1a, but not Asf1b, was necessary for the assembly of histone H3.3 in paternal pronuclei after fertilization.
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http://dx.doi.org/10.1186/s13072-021-00430-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670131PMC
December 2021

Application value between dynamic electrocardiogram and MSCT myocardial perfusion imaging in the diagnosis of myocardial ischemia in coronary heart disease.

Ann Palliat Med 2021 10;10(10):10720-10725

Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, China; Ultrasound in Cardiac Electrophysiology and Biomechanics Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Background: Dynamic electrocardiogram and multi-spiral computed tomography (MSCT) myocardial perfusion imaging are commonly used in clinical examination methods, which can provide important reference for the diagnosis of myocardial ischemia in coronary heart disease. To analyze and compare the application value of dynamic electrocardiogram and MSCT myocardial perfusion imaging in the diagnosis of myocardial ischemia in coronary heart disease.

Methods: The clinical data of 79 patients diagnosed with coronary heart disease admitted in our hospital from October 2017 to October 2019 were retrospectively collected. Exercise load/rest myocardial perfusion imaging (MPI) confirmed the presence of myocardial ischemia, and the patients were all examined by dynamic electrocardiogram and MSCT. The diagnosis of exercise load/resting MPI was used as a benchmark to compare the sensitivity, specificity, and accuracy of the different tests in the diagnosis of myocardial ischemia in coronary heart disease.

Results: As confirmed by the exercise load/rest MPI, the 79 included patients had myocardial ischemia. Fifty-five cases of myocardial ischemia were detected by dynamic electrocardiogram, of which 33 cases showed horizontal depression of the ST segment. Also, 15 cases exhibited downward oblique depression, and seven cases showed upward oblique depression. MSCT examination showed that 78 patients had myocardial ischemia, of which 74 showed abnormal myocardial perfusion when contracted. Of these patients, 47 showed reversible perfusion filling, 20 exhibited partially reversible perfusion filling, and seven showed fixed perfusion defects. The sensitivity, specificity, and accuracy of dynamic electrocardiogram in the diagnosis of myocardial ischemia in coronary heart disease were 60.76%, 72.15%, 69.62%, respectively, while those of MSCT were 91.14%, 75.94%, and 86.07%, respectively. The sensitivity and accuracy of MSCT examination were significantly higher than those of dynamic electrocardiogram examination (P<0.05), and the specificity was slightly higher than that of dynamic electrocardiogram examination, but this difference was not statistically significant (P>0.05).

Conclusions: Compared to dynamic electrocardiogram examination, MSCT myocardial perfusion imaging has superior sensitivity and accuracy in the diagnosis of myocardial ischemia in coronary heart disease, and can provide a reference for clinical diagnosis and treatment.
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http://dx.doi.org/10.21037/apm-21-2481DOI Listing
October 2021

AMOTL2 restrains transforming growth factor-β1-induced proliferation and extracellular matrix deposition of airway smooth muscle cells via the down-regulation of YAP1 activation.

Environ Toxicol 2021 Nov 29;36(11):2225-2235. Epub 2021 Jul 29.

Division of Pulmonary and Critical Care Medicine, the Second Affiliated Hospital of Xi'an Jiaotong University, Xian, China.

Angiomotin-like 2 (AMOTL2) is a key modulator of signaling transduction and participates in the regulation of various cellular progresses under diverse physiological and pathological conditions. However, whether AMOTL2 participates in asthma pathogenesis has not been fully studied. In the present work, we studied the possible role and mechanism of AMOTL2 in regulating transforming growth factor-β1 (TGF-β1)-induced proliferation and extracellular matrix (ECM) deposition of airway smooth muscle (ASM) cells. Our results showed marked reductions in the abundance of AMOTL2 in TGF-β1-stimulated ASM cells. Cellular functional investigations confirmed that the up-regulation of AMOTL2 dramatically decreased the proliferation and ECM deposition induced by TGF-β1 in ASM cells. In contrast, the depletion of AMOTL2 exacerbated TGF-β1-induced ASM cell proliferation and ECM deposition. Further research revealed that the overexpression of AMOTL2 restrained the activation of Yes-associated protein 1 (YAP1) in TGF-β1-stimulated ASM cells. Moreover, the reactivation of YAP1 markedly reversed AMOTL2-mediated suppression of TGF-β1-induced ASM cell proliferation and ECM deposition. Together, these findings suggest that AMOTL2 restrains TGF-β1-induced proliferation and ECM deposition of ASM cells by down-regulating YAP1 activation.
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http://dx.doi.org/10.1002/tox.23336DOI Listing
November 2021

Isolation and Identification of a Novel Phlebovirus, Hedi Virus, from Sandflies Collected in China.

Viruses 2021 04 27;13(5). Epub 2021 Apr 27.

State Key Laboratory of Infectious Disease Prevention and Control, Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.

We report the isolation of a newly recognized phlebovirus, Hedi virus (HEDV), from sandflies collected in Shanxi Province, China. The virus' RNA is comprised of three segments. The greatest amino acid sequence similarity of the three gene segments between this virus and previously recognized phleboviruses is 40.85-63.52%, and the RNA-dependent RNA polymerase (RdRp) amino acid sequence has the greatest similarity (63.52%) to the Rift Valley fever virus (RVFV) ZH-548 strain. Phylogenetic analysis of the amino acid sequence of the virus RdRp indicated that HEDV is close to RVFV and distinct from other phleboviruses, forming its own evolutionary branch. We conclude that it is necessary to increase the monitoring of phleboviruses carried by sandflies in China.
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http://dx.doi.org/10.3390/v13050772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8145316PMC
April 2021

Isolation and Characterization of Wuxiang Virus from Sandflies Collected in Yangquan County, Shanxi Province, China.

Vector Borne Zoonotic Dis 2021 06 23;21(6):446-457. Epub 2021 Apr 23.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

In this study, we isolated a group of viruses that can cause a cytopathic effect in BHK-21 cells from sandfly specimens collected in Yangquan County, Shanxi Province, central China. The representative virus strain is SXYQ1860 and, with increased viral injection, the suckling mice became ill and died, with viral titers continually increasing. The results of molecular genetic evolution analysis of the three gene segments of the virus, L, M, and S, indicate that the newly isolated viruses from Yangquan County are the same as the Wuxiang virus (WUXV) previously isolated from sandflies collected in Wuxiang county, China. This is the first time that the WUXV was also isolated outside Wuxiang County. Therefore, strengthening the surveillance of neglected sandflies and the viruses they transmit to help prevent and control arboviruses and the associated diseases is essential for public health.
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http://dx.doi.org/10.1089/vbz.2020.2699DOI Listing
June 2021

Associations of sleep duration and quality with incident cardiovascular disease, cancer, and mortality: a prospective cohort study of 407,500 UK biobank participants.

Sleep Med 2021 05 15;81:401-409. Epub 2021 Mar 15.

School of Public Health, Tianjin Medical University, Tianjin, China. Electronic address:

Objective: Few studies have investigated the associations of sleep duration and sleep quality with incident cardiovascular diseases (CVDs), cancer, and mortality in the same large population. This study aimed at estimating the independent risk factors of long or short sleep durations and several typical characteristics of poor sleep quality for incident CVDs, cancer, and mortality.

Methods: In this prospective cohort study, 407 500 individuals were enrolled. Cox proportional hazards models were used to calculate the adjusted hazard ratios and 95% confidence intervals (HR, 95%CI) of associations of sleep duration and quality with incident CVDs, cancer, and mortality.

Results: Compared with the sleep duration of 7 h, sleep duration of ≤5 h and ≥9 h were both associated with higher risk of all-cause mortality (HR = 1.25, 95% CI: 1.16-1.34 and HR = 1.30, 95% CI: 1.22-1.38, respectively), CVD mortality (HR = 1.27, 95% CI: 1.09-1.49 and HR = 1.32, 95% CI: 1.16-1.50, respectively), and CVD incidence (HR = 1.23, 95% CI: 1.16-1.31 and HR = 1.08, 95% CI: 1.02-1.15, respectively). Additionally, long sleep duration (≥9 h) was associated with a higher risk of cancer mortality (HR = 1.19, 95% CI: 1.10-1.30) and cancer incidence (HR = 1.08, 95% CI: 1.04-1.12). Moreover, CVD incidence was significantly associated with snoring, insomnia and narcolepsy, increasing the risk by 7%, 26%, and 20%, respectively.

Conclusion: Long sleep durations may substantially increase the risk of mortality and morbidity. Snoring, insomnia, and narcolepsy were independent risk factors for incident CVD.
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http://dx.doi.org/10.1016/j.sleep.2021.03.015DOI Listing
May 2021

Advantages of introducing an effective crystalline inhibitor in curcumin amorphous solid dispersions formulated by Eudragit E100.

J Pharm Pharmacol 2021 Mar;73(2):185-192

School of Pharmacy, Shenyang Medical College, Shenyang, PR China.

Objectives: This paper was to elucidate the advantages of using an effective crystalline inhibitor, which was hydroxypropyl methylcellulose E5 (HPMC), in inhibiting crystallisation for curcumin amorphous solid dispersion (Cur ASDs) formulated by Eudragit E100 (E100).

Methods: Physical characterisation such as differential scanning calorimetry and powder X-ray diffraction revealed the solid state during the formation of dispersion and clarified the compatibility between Cur and excipient.

Key Findings: The liquidity of excipient and the change of Tg in Cur ASDs demonstrated that the addition of HPMC can reduce molecule motion of the whole system, improve Tg of Cur ASDs and inhibit crystallisation of Cur ASDs. The water uptake experiment and molecular dynamic modelling further confirmed the effective solution and matrix crystallisation inhibition role of HPMC.

Conclusions: The elucidation of HPMC as auxiliary excipient on inhibiting crystallisation for Cur ASDs will bring huge value in designing Cur ASDs in the future.
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http://dx.doi.org/10.1093/jpp/rgaa012DOI Listing
March 2021

Molecular dynamics simulation studies on the specific regulation of PTPN18 to the HER2 phospho-peptides.

J Mol Recognit 2021 07 23;34(7):e2890. Epub 2021 Feb 23.

Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, China.

The specific regulation of PTPN18 protein to three HER2 phospho-peptides has been studied by molecular dynamics simulations and free energy calculations. The results revealed that the three HER2 phospho-peptides binding to the PTPN18 catalytic domain is energetically favorable due to substrate specificity of PTPN18, and moreover, the PTPN18 protein have significantly higher affinity to pY1248 peptide (-45.22 kcal/mol) than that of pY1112 (-25.3 kcal/mol) and pY1196 (-31.86 kcal/mol) peptides. Further, the binding of HER2 phospho-peptides to PTPN18 have also caused the closure of WPD-loop with the decrease of the centroid distances between the P-loop and the WPD loop. The WPD-loop closure of PTPN18 relates directly to the new hydrogen bond and hydrophobic interaction formations between the residues Tyr62, Asp64, Val65, Ala231, Arg235, and Ala273 in PTPN18 and Tyr(PO3) in the HER2 phospho-peptides, which suggests that these key residues would contribute to the specific regulation of PTPN18 to the substrates. The correlation analysis revealed the allosteric communication networks from the pY binding loop to the WPD loop through the structural change and the residue interactions in PTPN18. These results will be helpful to understand the specific regulation through the allosteric communication network in the PTPN18 catalytic domain.
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http://dx.doi.org/10.1002/jmr.2890DOI Listing
July 2021

Corrigendum to"ScPNP-A, a plant natriuretic peptide from Stellera chamaejasme, confers multiple stress tolerances in Arabidopsis" [Plant Physiol. Biochem. 149 (2020) 132-143].

Plant Physiol Biochem 2021 Mar 6;160:413. Epub 2021 Feb 6.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education & College of Life Sciences, Northwest University, No. 229, North Taibai Road, Xi'an, 710069, Shaanxi, China. Electronic address:

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http://dx.doi.org/10.1016/j.plaphy.2021.01.030DOI Listing
March 2021

Emerging Sand Fly-Borne Phlebovirus in China.

Emerg Infect Dis 2020 10;26(10):2435-2438

We isolated 17 viral strains capable of causing cytopathic effects in mammalian cells and death in neonatal mice from sand flies in China. Phylogenetic analysis showed that these strains belonged to the genus Phlebovirus. These findings highlight the need to control this potentially emerging virus to help safeguard public health.
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http://dx.doi.org/10.3201/eid2610.191374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510709PMC
October 2020

Emergence of Zika Virus in Culex tritaeniorhynchus and Anopheles sinensis Mosquitoes in China.

Virol Sin 2021 Feb 2;36(1):33-42. Epub 2020 Jul 2.

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

Zika virus (ZIKV) has been isolated from mosquitoes such as Aedes, Mansonia uniformis, and Culex perfuscus; However, the isolation of ZIKV from Anopheles sinensis and Culex tritaeniorhynchus has not yet been reported. In June and July 2018, 22,985 mosquitoes and 57,500 midges were collected in Jiangxi Province in southeastern China. Among them, six strains of ZIKV were isolated from mosquitoes: four from An. sinensis and two from Cx. tritaeniorhynchus. Molecular genetic analysis showed that the ZIKV isolated from An. sinensis and Cx. tritaeniorhynchus belonged to genotype 2 in the Asian evolutionary branch of ZIKV. In addition, the ZIKV strains isolated from An. sinensis and Cx. tritaeniorhynchus had amino acid substitutions identical to ZIKV strains prevalent in South America since 2015. This study is the first to isolate ZIKV from mosquito specimens collected in the wild of Jiangxi Province, China; This is also the first time that ZIKV has been isolated from An. sinensis and Cx. tritaeniorhynchus. Given that An. sinensis and Cx. tritaeniorhynchus have a very wide geographical distribution in China and even in eastern and southern Asia, the isolation of several strains of ZIKV from these two mosquitoes poses new challenges for the prevention and control of ZIKV infection in the mainland of China and countries and regions with the same distribution of mosquitoes.
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http://dx.doi.org/10.1007/s12250-020-00239-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973324PMC
February 2021

Hollow Porous MnFe O Sphere Grown on Elm-Money-Derived Biochar towards Energy-Saving Full Water Electrolysis.

Chemistry 2020 Nov 14;26(63):14397-14404. Epub 2020 Oct 14.

Faculty of Chemistry, Northeast Normal University, Changchun, 130024, P. R. China.

The development of inexpensive and efficient bifunctional electrocatalysts is significant for widespread practical applications of overall water splitting technology. Herein, a one-pot solvothermal method is used to prepare hollow porous MnFe O spheres, which are grown on natural-abundant elm-money-derived biochar material to construct MnFe O /BC composite. When the overpotential is 156 mV for both the oxygen evolution reaction and the hydrogen evolution reaction, the current density reaches up to 10 mA cm , and its duration is 10 h. At 1.51 V, the overall water decomposition current density of 10 mA cm can be obtained in 1 m KOH. This work proves that elm-money-derived biochar is a valid substrate for growing hollow porous spheres. MnFe O /BC give a promising general strategy for preparing the effective and stable bifunctional catalysis that can be expand to multiple transition metal oxide.
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http://dx.doi.org/10.1002/chem.202002134DOI Listing
November 2020

Strategy Use in Second Language Vocabulary Learning and Its Relationships With the Breadth and Depth of Vocabulary Knowledge: A Structural Equation Modeling Study.

Authors:
Na Fan

Front Psychol 2020 13;11:752. Epub 2020 May 13.

Department of English, School of International Studies, Shaanxi Normal University, Xi'an, China.

This study investigated Chinese English-as-a-foreign-language (EFL) learners' use of vocabulary learning strategies (VLSs) and its relationship with vocabulary knowledge (VK), especially in relation to proficiency, gender, and discipline. Structural equation models were established following exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) procedures, and mediation analyses and multiple-group analyses, as well as analyses of variance, were conducted. Four hundred nineteen sophomores' strategy use frequency, Vocabulary Size Test (VST) scores (indicative of breadth of VK), Word Associates Test (WAT) scores (indicative of depth of VK), College English Test Band-4 scores, and gender and discipline categories were used as data. Proficiency significantly predicted Attention and Guessing positively but was a negative predictor of Socializing (asking others for help). Girls liked making notes while using dictionaries (DictNote) and Socializing, and students of arts also took more notes. Attention and Guessing significantly predicted VST and WAT positively, but Socializing significantly predicted the breadth and depth of VK negatively, and DictNote, Association, and Repetition had no significant relationship. The predictive power of Attention, Guessing, and Socializing, however, was achieved mainly, or for an important part, via the mediating or indirect effects of proficiency. Gender moderated the predictive power of Attention, Socializing, and DictNote over VST, greater for male students, whereas discipline moderated the relationship between Guessing and WAT, stronger for arts students. The findings are related to strategy features, gender characteristics, disciplinary influence, the EFL context and culture, and effective learning. This study reveals the complex relationships among use of VLSs, VK, and learner variables. Attention is called for to third-party factors in understanding VLSs-VK relationships. Given the important mediating effects of proficiency, it is proposed that vocabulary learning be strategically integrated into the accumulative process of English learning.
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http://dx.doi.org/10.3389/fpsyg.2020.00752DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7237738PMC
May 2020

Imaging characteristics of initial chest computed tomography and clinical manifestations of patients with COVID-19 pneumonia.

Jpn J Radiol 2020 Jun 21;38(6):533-538. Epub 2020 Apr 21.

Department of Radiology, General Hospital of the Yangtze River Shipping, Wuhan, Hubei Province, China.

Purpose: To investigate the chest CT imaging characteristics and clinical manifestations of patients with COVID-19 pneumonia.

Methods: This study included 150 patients with COVID-19 pneumonia diagnosed from January 10 to February 12, 2020 to analyze their clinical and CT imaging characteristics.

Results: The period between symptom onset and initial CT examination ranged from 1 to 8 days. There were 83 cases (55.33%) involving both lungs, 67 cases (44.67%) involving a single lung (left 25 cases and right 42 cases). There were 49 cases (32.67%) of single intrapulmonary lesion, 33 cases (22.00%) of multiple intrapulmonary lesions, 68 cases (44.00%) of diffused intrapulmonary lesions, 67 cases (44.67%) of subpleural lesions, 24 cases (16.00%) of lesions localizing along the bronchovascular bundles, and 59 cases (39.33%) with lesions in both locations. There were 18 cases (12.00%) exhibiting ground-glass nodules of < 10 mm, 124 cases (82.67%) of patchy ground-glass opacities with or without consolidation, 8 cases (5.33%) of cord-like lesions, 6 cases (4.00%) of pleural effusion, and 2 cases (1.33%) of enlarged lymph nodes.

Conclusions: The main manifestations of initial chest CT in COVID-19 pneumonia patients was ground-glass opacities, commonly involving single site in patients < 35 years old and multiple sites and extensive area in patients > 60 years old. The common lesion sites were the subpleural region and the posterior basal segments of the lower lobes, mostly showing thickening of the interlobular septum and mixed with consolidation.
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http://dx.doi.org/10.1007/s11604-020-00973-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7171599PMC
June 2020

Amino functionalized chiral mesoporous silica nanoparticles for improved loading and release of poorly water-soluble drug.

Asian J Pharm Sci 2019 Jul 1;14(4):405-412. Epub 2018 Jul 1.

Wuya college of Innovation, Shenyang Pharmaceutical University, No.103, Wenhua Road, Shenyang 110016, China.

In the present paper, chiral mesoporous silica nano-cocoon (A-CMSN) functionalized with amino group was synthesized, and its loading and release of indomethacin (IMC), a poorly soluble drug, was studied. Due to the use of chiral anionic surfactants as a template, A-CMSN possessed 2D hexagonal nano-cocoon morphology with curled channels on its surface, which was quite different from another 2D hexagonal mesoporous silica nanoparticles (MCM-41) with straightway channels. After being loaded into the two silica carriers by hydrogen bond, crystalline IMC converted to amorphous form, leading to the improved drug dissolution. And IMC loading capacity of A-CMSN was higher than MCM-41 because curled loading process originating from curvature chiral channels can hold more drug molecules. Compared with IMC, IMC loaded A-CMSN presented obviously fast release throughout the release experiment, while IMC loaded MCM-41 released faster than IMC at the initial 5 h then showed controlled slow release afterwards, which was closely related to the mesoporous silica nanoparticles and different channel mesostructures of these two carriers. A-CMSN possessed nano-cocoon morphology with curled 2D hexagonal channel and its channel length was shorter than MCM-41, therefore IMC molecules can easily get rid of the constraint of A-CMSN then to be surrounded by dissolution medium.
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http://dx.doi.org/10.1016/j.ajps.2018.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7032221PMC
July 2019

ScPNP-A, a plant natriuretic peptide from Stellera chamaejasme, confers multiple stress tolerances in Arabidopsis.

Plant Physiol Biochem 2020 Apr 7;149:132-143. Epub 2020 Feb 7.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education & College of Life Sciences, Northwest University, No. 229, North Taibai Road, Xi'an, 710069, Shaanxi, China. Electronic address:

As a class of peptide hormone, plant natriuretic peptides (PNPs) play an important role in maintaining water and salt balance in plants, as well as in the physiological processes of biotic stress and pathogen resistance. However, in plants, except for some PNPs, such as the Arabidopsis thaliana PNP-A (AtPNP-A), of which the function has not yet been thoroughly revealed, few PNPs in other plants have been reported. In this study, a PNP-A (ScPNP-A) has been identified and characterized in Stellera chamaejasme for the first time. ScPNP-A is a double-psi beta-barrel (DPBB) fold containing protein and is localized in the extracellular (secreted) space. In S. chamaejasme, the expression of ScPNP-A was significantly up-regulated by salt, drought and cold stress. Changes at the physiological and biochemical levels and the expression of resistance-related genes indicated that overexpression of ScPNP-A can significantly improve salt, drought and freezing tolerance in Arabidopsis. ScPNP-A could stimulate the opening, not the closing of stomata, and its expression was not enhanced by external application of ABA. Furthermore, overexpression of ScPNP-A resulted in the elevated expression of genes in the ABA biosynthesis and reception pathway. These suggested that there may be some cross-talk between ScPNP-A and the ABA-dependent signaling pathways to regulate water related stress, however further experimentation is required to understand this relationship. In addition, overexpression of ScPNP-A can enhance the resistance to pathogens by enhancing SAR in Arabidopsis. These results indicate that ScPNP-A could function as a positive regulator in plant response to biotic stress and abiotic stress.
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http://dx.doi.org/10.1016/j.plaphy.2020.02.005DOI Listing
April 2020

Quercetin amorphous solid dispersions prepared by hot melt extrusion with enhanced solubility and intestinal absorption.

Pharm Dev Technol 2020 Apr 7;25(4):472-481. Epub 2020 Jan 7.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.

The aim of this study was to investigate the effect of amorphous solid dispersions (ASDs) on the dissolution rate and oral bioavailability of Quercetin (Que). First, we prepared the Que ASDs with various excipients using hot-melt extrusion to find the best option. X-ray diffraction (XRD), infrared spectroscopy (IR), and Raman spectroscopy were used to examine the solid formation of Que. Wetting process was studied by contact angle and solution process. The abilities of HPMC to inhibit crystallization and improve membrane permeability were demonstrated by fluorescence spectroscopy, dynamic light scattering analysis, permeability experiment and pharmacokinetics studies. Que existed as amorphous in solid dispersions, and poloxamer 188 (F68) was the best excipient for improving Que dissolution. Study on ASDs wettability proved Que ASDs improved wetting property in the presence of the F68. Furthermore, Que/F68/HPMC 1/4/3 and 1/5/2 ASDs belonged to drug-controlled diffusion; Que/F68/HPMC 1/6/1 ASDs belonged to drug/carrier-controlled diffusion; Que/F68 1/7 ASDs belonged to carrier-controlled diffusion. Addition of HPMC significantly inhibited the crystallization, improved membrane permeability and promoted drug absorption of compound Que. Que ASDs prepared enhanced solubility and intestinal absorption. Thus, Que ASDs provide a potent and efficacious formulation for Que oral administration.
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http://dx.doi.org/10.1080/10837450.2019.1709502DOI Listing
April 2020

Surface Tracking of Curcumin Amorphous Solid Dispersions Formulated by Binary Polymers.

J Pharm Sci 2020 02 19;109(2):1068-1078. Epub 2019 Oct 19.

School of Basic Medical Science, Shenyang Medical College, Shenyang, Liaoning, China. Electronic address:

Herein, curcumin amorphous solid dispersions (Cur ASDs) were prepared using binary polymers (Eudragit EPO with polyvinylpyrrolidone K30 [EuD-PVP], Eudragit EPO with hydroxypropyl methylcellulose E50 [EuD-HPMC]) as excipient, and surface tracking of Cur ASDs was mainly addressed. Infrared spectroscopy, in situ Raman imaging spectroscopy, molecular docking modeling, and contact angle measurements were mainly applied to study the molecular interaction and wetting property of Cur ASDs. Cur/EuD-PVP had a lower cumulative release (approximately 15%) than Cur/EuD-HPMC (approximately 48%) because Cur/EuD-HPMC presented carrier-controlled wetting property while Cur/EuD-PVP fitted to a drug-controlled wetting property. The favorable HPMC can be preferred as superior excipient for Cur ASDs compared with binary polymers, which has potential application in food and drug healthcare industry of Cur.
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http://dx.doi.org/10.1016/j.xphs.2019.10.030DOI Listing
February 2020

The subcortical maternal complex protein Nlrp4f is involved in cytoplasmic lattice formation and organelle distribution.

Development 2019 10 18;146(20). Epub 2019 Oct 18.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China

In mammalian oocytes and embryos, the subcortical maternal complex (SCMC) and cytoplasmic lattices (CPLs) are two closely related structures. Their detailed compositions and functions remain largely unclear. Here, we characterize Nlrp4f as a novel component associated with the SCMC and CPLs. Disruption of maternal Nlrp4f leads to decreased fecundity and delayed preimplantation development in the mouse. Lack of Nlrp4f affects organelle distribution in mouse oocytes and early embryos. Depletion of Nlrp4f disrupts CPL formation but does not affect the interactions of other SCMC proteins. Interestingly, the loss of Khdc3 or Tle6, two other SCMC proteins, also disrupts CPL formation in mouse oocytes. Thus, the absence of CPLs and aberrant distribution of organelles in the oocytes caused by disruption of the examined SCMC genes, including previously reported , , and , indicate that the SCMC is required for CPL formation and organelle distribution. Consistent with the role of the SCMC in CPL formation, the SCMC forms before CPLs during mouse oogenesis. Together, our results suggest that the SCMC protein Nlrp4f is involved in CPL formation and organelle distribution in mouse oocytes.
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http://dx.doi.org/10.1242/dev.183616DOI Listing
October 2019

Three new bioactive flavonoid glycosides from Viscum album.

Chin J Nat Med 2019 Jul;17(7):545-550

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.

Two new flavonoid glycosides, named viscumneoside XII (1), and viscumneoside XIII (2); a new dihydrogen flavonoid glycoside product named viscumneoside XIV (3), were isolated from the aerial part of Viscum album, along with seven known compounds (4-10). Their structures were identified by analysis of spectroscopic data. In addition, cytotoxicity assay showed that 1, 2 and 3 possessed significant inhibitory activities against C6, A549 and MDA-MB-231 (the inhibition rate arrived about 50%, 70% and 74% respectively with IC ≤ 60.00 μmol·L), while the inhibition of TF-1 and Hela was not significant.
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http://dx.doi.org/10.1016/S1875-5364(19)30076-7DOI Listing
July 2019

Linear Control of Moisture Permeability and Anti-adhesion of Bacteria in a Broad Temperature Region Realized by Cross-Linking Thermoresponsive Microgels onto Cotton Fabrics.

ACS Appl Mater Interfaces 2019 Aug 9;11(33):30269-30277. Epub 2019 Aug 9.

Key Laboratory of Advanced Textile Materials & Manufacturing Technology, Ministry of Education , Zhejiang Sci-Tech University , 310018 Hangzhou , China.

Linear control of moisture permeability and anti-adhesion of bacteria in a broad temperature region are realized by cross-linking thermoresponsive microgels onto cotton fabrics. The microgels are copolymerized by monomers di(ethylene glycol) methyl ether methacrylate (MEOMA), (ethylene glycol) methyl ether methacrylate (OEGMA), and ethylene glycol methacrylate (EGMA) with a molar ratio of 10:10:1. Transition temperatures of PMEOMA and POEGMA are 25 and 60 °C, respectively. Due to the compression of already collapsed PMEOMA to still swollen POEGMA, the microgels present a linear shrinkage in a broad temperature region (20-70 °C). Additionally, the contact angle of the microgels stays below 60° even if the temperature is increased to 50 °C, illustrating the reserved surface hydrophilicity. The obtained microgels are cross-linked onto cotton fabrics by 1,2,3,4-butanetetracarboxylic (BTCA). The weight gain ratios (WGRs) are 15% and 30%. The moisture permeability shows an excellent linear increase between 20 and 50 °C when the WGR is 30%, which is attributed to the linear shrinkage of the cross-linked microgels upon heating. Because the moisture permeability is related to the fabric comfort, a linear control of comfort is obtained. In addition, the cross-linked cotton fabrics can realize 96.5% bacterial anti-adhesion at 30 °C as the surface remains hydrophilic. On the basis of these two unique properties, the realized cotton fabrics cross-linked with microgels are promising for application as smart textiles for wound addressing.
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http://dx.doi.org/10.1021/acsami.9b09294DOI Listing
August 2019

, a NAC-Type Transcription Factor, Conferring Enhanced Drought and Salt Stress Tolerance in .

Front Plant Sci 2019 11;10:890. Epub 2019 Jul 11.

Department of Life Science, Key Laboratory of Resource Biology and Biotechnology in Western China, Northwest University, Xi'an, China.

The NAC proteins form one of the largest families of plant-specific transcription factors (TFs) and play essential roles in developmental processes and stress responses. In this study, we characterized a NAC domain transcription factor, , from a legume . OoNAC72 was proved to be localized in the nuclei in tobacco lower epidermal cells and had transcriptional activation activity in yeast, confirming its transcription activity. expression could be induced by drought, salinity and exogenous abscisic acid (ABA) in seedlings. Furthermore, over-expression of driven by CaMV35S promoter in resulted in ABA hypersensitivity and enhanced tolerance to drought and salt stresses during seed germination and post-germinative growth periods. In addition, over-expression of enhanced the expression of stress-responsive genes such as , , , , , , , and . These results highlight the important regulatory role of in multiple abiotic stress tolerance, and may provide an underlying reason for the spread of .
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http://dx.doi.org/10.3389/fpls.2019.00890DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6637385PMC
July 2019

The On-Off chiral mesoporous silica nanoparticles for delivering achiral drug in chiral environment.

Colloids Surf B Biointerfaces 2019 Apr 26;176:122-129. Epub 2018 Dec 26.

Liaoning Key Laboratory of Environmental Pollution and Microecology, School of Basic Medical Science, Shenyang Medical College, No. 146 Huanghe North Street, Shenyang, PR China. Electronic address:

In the current stage, the On-Off mechanism of chiral mesoporous silica nanoparticles (CMSN) for delivering achiral drug in chiral environment has rarely been reported. Herein, On-Off chiral mesoporous silica nanoparticles (On-Off-D-CMSN and On-Off-L-CMSN) were successfully synthesized and its particular contribution in delivering achiral drug indometacin (IMC) in chiral environment was mainly studied. The as-synthesized On-Off-D-CMSN and On-Off-L-CMSN were verified by fourier transform infrared spectrometer and circular dichroism. The transmission electron microscope test and nitrogen adsorption/desorption analysis showed that On-Off-D-CMSN and On-Off-L-CMSN were regular spheres with concealed pore channels. The zeta potential analysis demonstrated that the grafting functional groups distributed on the inner and outer surfaces of On-Off-D-CMSN and On-Off-L-CMSN. Through dissolution experiment, the drug release of IMC loaded On-Off-D-CMSN (84%) and IMC loaded On-Off-L-CMSN (70%) were about 2.4 and 2.0 times higher than pure IMC (35%) in pH 6.8 phosphate buffer solution (PBS), respectively. IMC loaded On-Off-D-CMSN and IMC loaded On-Off-L-CMSN exerted different chiral recognition functions with On-Off mechanism in in-vitro chiral environment (pH 6.8 PBS-L and pH 6.8 PBS-D). The results of anti-inflammation pharmacodynamics further demonstrated that On-Off-D-CMSN and On-Off-L-CMSN can trigger chiral biological environment to achieve on or off chiral recognition functions. The unique advantages of On-Off chiral mesoporous silica nanoparticles in triggering chiral biological environment can provide valuable instruction for designing drug delivery system.
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http://dx.doi.org/10.1016/j.colsurfb.2018.12.065DOI Listing
April 2019

New advances in microfluidic flow cytometry.

Electrophoresis 2018 Sep 22. Epub 2018 Sep 22.

School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, Chengdu, P. R. China.

In recent years, researchers are paying the increasing attention to the development of portable microfluidic diagnostic devices including microfluidic flow cytometry for the point-of-care testing. Microfluidic flow cytometry, where microfluidics and flow cytometry work together to realize novel functionalities on the microchip, provides a powerful tool for measuring the multiple characteristics of biological samples. The development of a portable, low-cost, and compact flow cytometer can benefit the health care in underserved areas such as Africa or Asia. In this article, we review recent advancements of microfluidics including sample pumping, focusing and sorting, novel detection approaches, and data analysis in the field of flow cytometry. The challenge of microfluidic flow cytometry is also examined briefly.
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http://dx.doi.org/10.1002/elps.201800298DOI Listing
September 2018

Numerical and experimental investigation of 'water fan' effect due to electrohydrodynamic force in a microchamber.

Electrophoresis 2018 Sep 5. Epub 2018 Sep 5.

School of Engineering, University of Guelph, Guelph, Canada.

Electrohydrodynamics is commonly used in microfluidics to control and manipulate the fluid. Though there are studies on the rotation flow in suspended films, the thin film liquid is easily broken and cannot last long hence not applicable in specific applications. Here, we established a three-dimensional microchamber embedded with two pairs of microelectrodes to investigate the rotational phenomenon of bulk of liquid which we called 'water fan' effect based on the electrohydrodynamics force. When proper voltages were applied on these microelectrodes, the tornado-like rotation would be generated. Both the numerical and experimental results showed that the controllable and continuous rotation could be achieved in the microchamber. In addition, the concentration effect resulting from the rotation flow was also observed. The proposed method offers great promises in providing theoretical and practical guideline in microfluidic devices for mixing, separating, and cooling applications.
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http://dx.doi.org/10.1002/elps.201800269DOI Listing
September 2018
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