Publications by authors named "Na Chen"

589 Publications

Identification of Endoplasmic Reticulum Stress-Related Subtypes, Infiltration Analysis of Tumor Microenvironment, and Construction of a Prognostic Model in Colorectal Cancer.

Cancers (Basel) 2022 Jul 8;14(14). Epub 2022 Jul 8.

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.

Recently, endoplasmic reticulum (ER) stress has been shown to influence tumor progression and immune cell function in the tumor microenvironment (TME). However, the underlying role of ER stress-related gene patterns in colorectal cancer (CRC) development remains unclear. We analyzed the ER stress-related gene patterns in 884 patients with CRC from the Gene Expression Omnibus database and evaluated the cell-infiltrating patterns in the TME. Two ER stress-related patterns were identified in patients with CRC that had distinct cell-infiltrating patterns in the TME and clinical characteristics. A risk score and nomogram based on 14 screened prognosis-correlated genes was built and validated to predict patient survival. Patients with a higher risk score were shown to have an unfavorable prognosis, and the risk score was associated with cell infiltration and drug sensitivity. Furthermore, spatial transcriptomics data were utilized to explore ER stress-related gene patterns in CRC tissues, and it was shown that ER stress phenotype involves in the formation of the immunosuppressive TME. This study demonstrated that ER stress-related gene patterns play a role in influencing the TME and predicting prognosis. These analyses of ER stress in the TME of CRC might deepen our understanding of CRC progression and immune escape and provide novel insights into therapeutic strategies.
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http://dx.doi.org/10.3390/cancers14143326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322646PMC
July 2022

Neutrophils Promote Glioblastoma Tumor Cell Migration after Biopsy.

Cells 2022 Jul 14;11(14). Epub 2022 Jul 14.

Department of Respiratory Medicine, Center of Translational Immunology, University Medical Center Utrecht, Lundlaan 6, Mail room KC02.085.2, 3584 EA Utrecht, The Netherlands.

Glioblastoma is diagnosed by biopsy or, if clinically feasible, tumor resection. However, emerging evidence suggests that this surgical intervention may increase the risk of tumor cell spread. It has been hypothesized that the damage to the tumor leads to infiltration of immune cells that consequently form an environment that favors tumor cell motility. In mouse glioma models, it was previously found that biopsy induced migration of tumor cells in vivo and that recruitment of monocytes from the blood was involved in this effect. However, the role of neutrophils in this process is still unclear. Here, we study the contribution of neutrophils on the pro-migratory effect of surgical interventions in glioma. Using repetitive intravital microscopy, in vivo migration of glioma tumor cells before and after biopsy was compared in mice systemically depleted of neutrophils. Interestingly, macrophages/microglia were almost completely absent from neutrophil-depleted tumors, indicating that neutrophils may be indirectly involved in biopsy-induced migration of glioma tumor cells through the recruitment of macrophages to the tumor. To further investigate whether neutrophils have the potential to also directly promote glioblastoma tumor cell migration, we performed in vitro migration assays using human neutrophils. Indeed, wound-healing of human primary glioblastoma tumor cell lines was promoted by human neutrophils. The pro-migratory effects of human neutrophils on glioblastoma tumor cells could also be recapitulated in transwell migration assays, indicating that soluble factor(s) are involved. We therefore provide evidence for both an indirect and direct involvement of neutrophils in tumor spread following biopsy of glioblastoma tumors.
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http://dx.doi.org/10.3390/cells11142196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324761PMC
July 2022

Body mass index and trajectories of the cognition among Chinese middle and old-aged adults.

BMC Geriatr 2022 07 23;22(1):613. Epub 2022 Jul 23.

School of Health Economics and Management, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People's Republic of China.

This study aims to investigate the association between trajectories of the cognition and body mass index (BMI) among Chinese middle and old-aged adults. A total of 5693 adults (age 45 +) whose cognitive score is higher than average at the baseline were included from China Health and Retirement Longitudinal Study (CHARLS:2011-2015). Cognitive function was measured by Mini-mental state examination (MMSE) in Chinese version. The Group-based trajectory modeling (GBTM) was adopted to identify the potential heterogeneity of longitudinal changes over the past 5 years and to investigate the relationship between baseline BMI and trajectories of cognitive function. Three trajectories were identified in results: the slow decline (37.92%), the rapid decline (6.71%) and the stable function (55.37%). After controlling for other variables, underweight (BMI < 18.5 kg/m) was associated with the rapid and slow decline trajectories. Obesity (BMI > 28 kg/m) was associated with the slow decline trajectory. High-risk people of cognitive decline can be screened by measuring BMI.
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http://dx.doi.org/10.1186/s12877-022-03301-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308927PMC
July 2022

CBLRR: a cauchy-based bounded constraint low-rank representation method to cluster single-cell RNA-seq data.

Brief Bioinform 2022 Jul 24. Epub 2022 Jul 24.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, China.

The rapid development of single-cel+l RNA sequencing (scRNA-seq) technology provides unprecedented opportunities for exploring biological phenomena at the single-cell level. The discovery of cell types is one of the major applications for researchers to explore the heterogeneity of cells. Some computational methods have been proposed to solve the problem of scRNA-seq data clustering. However, the unavoidable technical noise and notorious dropouts also reduce the accuracy of clustering methods. Here, we propose the cauchy-based bounded constraint low-rank representation (CBLRR), which is a low-rank representation-based method by introducing cauchy loss function (CLF) and bounded nuclear norm regulation, aiming to alleviate the above issue. Specifically, as an effective loss function, the CLF is proven to enhance the robustness of the identification of cell types. Then, we adopt the bounded constraint to ensure the entry values of single-cell data within the restricted interval. Finally, the performance of CBLRR is evaluated on 15 scRNA-seq datasets, and compared with other state-of-the-art methods. The experimental results demonstrate that CBLRR performs accurately and robustly on clustering scRNA-seq data. Furthermore, CBLRR is an effective tool to cluster cells, and provides great potential for downstream analysis of single-cell data. The source code of CBLRR is available online at https://github.com/Ginnay/CBLRR.
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http://dx.doi.org/10.1093/bib/bbac300DOI Listing
July 2022

Targeted demethylation of the SLC5A7 promotor inhibits colorectal cancer progression.

Clin Epigenetics 2022 07 20;14(1):92. Epub 2022 Jul 20.

Department of Gastrointestinal Surgery, West China Hospital and State Key Laboratory of Biotherapy, Sichuan University, Chengdu, 610041, Sichuan, People's Republic of China.

Background: SLC5A7 (solute carrier family 5 member 7), also known as choline transporter 1 (CHT1), is downregulated in colorectal cancer (CRC) and functions as a tumor suppressor. However, the mechanisms underlying the inactivation of SLC5A7 in CRC remain to be elucidated.

Results: In the present study, two broad-spectrum demethylation agents (azacitidine and decitabine) employed to treat CRC cells significantly upregulated SLC5A7 expression. Further results based on the CRC cohort and TCGA database indicated that SLC5A7 promoter methylation inversely correlated with SLC5A7 expression, and the status of SLC5A7 promotor methylation showed a promising prognostic value for patients with CRC. Next, the dCas9-multiGCN4/scFv-TET1CD-based precision demethylation system was constructed, which could significantly and specifically promote SLC5A7 expression in CRC cells through sgRNA targeting the SLC5A7 promoter. Both in vitro and in vivo experiments demonstrated that targeted demethylation of SLC5A7 by dCas9-multiGCN4/scFv-TET1CD-sgSLC5A7 inhibited tumor growth by stabilizing p53 and regulating downstream targets.

Conclusions: Collectively, DNA promoter methylation caused inactivation of SLC5A7 in CRC, and targeted demethylation of SLC5A7 might be a therapeutic target for CRC and other cancers.
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http://dx.doi.org/10.1186/s13148-022-01308-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9301853PMC
July 2022

Rotavirus and Norovirus Infections in Children Under 5 Years Old with Acute Gastroenteritis in Southwestern China, 2018-2020.

J Epidemiol Glob Health 2022 Jul 20. Epub 2022 Jul 20.

Yunnan Key Laboratory of Vaccine Research & Development On Severe Infectious Disease, Department of Immunogenetics, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, 650118, Yunnan, People's Republic of China.

Objective: Rotaviruses and noroviruses are important causes of acute gastroenteritis in children. While previous studies in China have mainly focused on rotavirus, we investigated the incidence of norovirus in addition to rotavirus in Southwestern China.

Methods: From January 2018 to December 2020, cases of rotavirus or norovirus infections among children under five ages with acute gastroenteritis were evaluated retrospectively.

Results: The detection rate of rotavirus was 24.5% (27,237/111,070) and norovirus was 26.1% (4649/17,797). Among 17,113 cases submitted for dual testing of both rotavirus and norovirus, mixed rotavirus/norovirus infections were detected in 5.0% (859/17,113) of cases. While there was no difference in norovirus incidence in outpatient compared to hospitalized cases, rotavirus was detected two times more in outpatients compared to hospitalized cases (26.6% vs.13.6%; P < 0.001). Both rotavirus and norovirus infections peaked in children aged 12-18 months seeking medical care with acute gastroenteritis (35.6% rotavirus cases; 8439/23,728 and 32.5% norovirus cases; 1660/5107). Rotavirus infections were frequent between December and March of each year while norovirus was detected earlier from October to December. Our results showed significant correlation between virus detection and environmental factors such as average monthly temperature but not relative humidity. In addition, we observed a reduction in the detection rates of rotavirus and norovirus at the beginning of the SARS-CoV-2 pandemic in 2020.

Conclusion: Our results indicate that rotavirus and norovirus are still important viral agents in pediatric acute gastroenteritis in Southwestern China.
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http://dx.doi.org/10.1007/s44197-022-00050-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297278PMC
July 2022

[Simultaneous determination of glucosinolates and flavonoids in leaves of Moringa oleifera by quantitative analysis of multi-components by single-marker (QAMS)].

Zhongguo Zhong Yao Za Zhi 2022 Jun;47(12):3285-3294

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

To establish a method for simultaneously determining the content of four glucosinolates and five flavonoids in leaves of Moringa oleifera via quantitative analysis of multi-components by single-marker(QAMS) and verify the feasibility and applicability of the established method. The glucosinolates and flavonoids were analyzed via Waters Acquity UPLC HSS T_3 column(2.1 mm × 100 mm, 1.8 μm). The gradient elution was carried out with the mobile phase composed of 0.1% formic acid-acetonitrile and 0.3% formic acid at the flow rate of 0.4 mL·min~(-1) and the column temperature of 40 ℃. The wavelengths for the detection of glucosinolates and flavonoids were 225 nm and 260 nm, respectively. With 4-O-(α-L-rhamnoyloxy)-benzyl glucosinolate and vecenin-2 as internal reference substances, the relative correction factors of four glucosinolates and five flavonoids were respectively calculated for determining the content of the 9 ingredients in leaves of M. oleifera. To verify the accuracy and feasibility of QAMS, we used internal standard method(ISM) and external standard method(ESM) to determine the content of glucosinolates and flavonoids, respectively. The t-test results showed that there was no significant difference in the content of glucosinolates obtained by ISM and QAMS methods or in the content of flavonoids obtained by ESM and QAMS methods. The content of glucosinolates and flavonoids varied among M. oleifera of different varieties and from different producing areas. The total glucosinolates and total flavonoids had the highest content in the Indian variety while the lowest content in the variety ■Honghe No. 1'. The established QAMS method is rapid, simple and accurate and can be used for simultaneous determination of glucosinolates and flavonoids in the leaves of M. oleifera. This study provides experimental data for the quality control and utilization of M. oleifera leaves.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211214.203DOI Listing
June 2022

Penpulimab, an Fc-Engineered IgG1 Anti-PD-1 Antibody, With Improved Efficacy and Low Incidence of Immune-Related Adverse Events.

Front Immunol 2022 27;13:924542. Epub 2022 Jun 27.

Research and Development Department, Akeso Biopharma Co., Ltd., Zhongshan, China.

Background: IgG4 anbibodies are deficient in stability and may contribute to tumor-associated escape from immune surveillance. We developed an IgG1 backbone anti-programmed cell death protein-1 (PD-1) antibody, penpulimab, which is designed to remove crystallizable fragment (Fc) gamma receptor (FcγR) binding that mediates antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP) and proinflammatory cytokine release.

Methods: Aggregation of different anti-PD-1 antibodies was tested by size exclusion chromatography, and melting temperature midpoint (Tm) and aggregation temperature onset (Tagg) were also determined. The affinity constants of penpulimab for PD-1 and human FcγRs were measured by surface plasmon resonance and biolayer interferometry. ADCC and ADCP were determined in cellular assays and antibody-dependent cytokine release (ADCR) from human macrophages was detected by ELISA. Binding kinetics of penpulimab to human PD-1 was determined by Biacore, and epitope/paratope mapping of PD-1/penpulimab was investigated using x-ray crystallography. Additionally, patients from six ongoing trials were included for analysis of immune-related adverse events (irAEs).

Results: Penpulimab demonstrated better stability and a lower level of host-cell protein residue compared with IgG4 backbone anti-PD-1 antibodies. As expected, penpulimab exhibited no apparent binding to FcγRIa, FcγRIIa_H131, FcγRIIIa_V158 and FcγRIIIa_F158, elicited no apparent ADCC and ADCP activities, and induced no remarkable IL-6 and IL-8 release by activated macrophages . Penpulimab was shown in the co-crystal study to bind to human PD-1 -glycosylation site at N58 and had a slower off-rate from PD-1 nivolumab or pembrolizumab. Four hundred sixty-five patients were analyzed for irAEs. Fifteen (3.2%) patients had grade 3 or above irAEs. No death from irAEs was reported.

Conclusions: IgG1 backbone anti-PD1 antibody penpulimab has a good stability and reduced host cell protein residue, as well as potent binding to the antigen. Fc engineering has eliminated Fc-mediated effector functions of penpulimab including ADCC, ADCP and reduced ADCR, which may contribute to its more favorable safety profile.

Clinical Trial Registration: www.ClinicalTrials.gov, identifier: AK105-101: NCT03352531, AK105-201: NCT03722147, AK105-301: NCT03866980, AK105-202:NCT03866967, AK105-203: NCT04172571, AK105-204: NCT04172506.
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http://dx.doi.org/10.3389/fimmu.2022.924542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9272907PMC
July 2022

An Electrochemical Strategy for Simultaneous Heavy Metal Complexes Wastewater Treatment and Resource Recovery.

Environ Sci Technol 2022 Aug 13;56(15):10945-10953. Epub 2022 Jul 13.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Environmental & Applied Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.

Heavy metals chelated with coexisting organic ligands in wastewater impose severe risks to public health and the ambient ecosystem but are also valuable metal resources. For sustainable development goals, the treatment of heavy metal complexes wastewater requires simultaneous metal-organic bond destruction and metal resource recovery. In this study, we demonstrated that a neutral pH electro-Fenton (EF) system, which was composed of an iron anode, carbon cloth cathode, and sodium tetrapolyphosphate electrolyte (NaTPP), could induce a successive single-electron activation pathway of molecular oxygen due to the formation of Fe(II)-TPP complexes. The boosted •OH generation in the NaTPP-EF process could decomplex 99.9% of copper ethylene diamine tetraacetate within 8 h; meanwhile, the released Cu ions were in situ deposited on the carbon cloth cathode in the form of Cu nanoparticles with a high energy efficiency of 2.45 g kWh. Impressively, the recovered Cu nanoparticles were of purity over 95.0%. More importantly, this neutral EF strategy could realize the simultaneous removal of Cu, Ni, and Cr complexes from real electroplating effluents. This study provides a promising neutral EF system for simultaneous heavy metal complexes wastewater treatment and resource recovery and sheds light on the importance of molecular oxygen activation in the field of pollutant control.
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http://dx.doi.org/10.1021/acs.est.2c02363DOI Listing
August 2022

Medium and long-term impact of SARS on total factor productivity(TFP): Empirical evidence from Chinese industrial enterprises.

J Asian Econ 2022 Oct 4;82:101507. Epub 2022 Jul 4.

School of Urban and Regional Science, Shanghai University of Finance and Economics, China.

This study examines the impact of the 2003 SARS epidemic on the total factor productivity (TFP) of Chinese industrial enterprises using a difference-in-differences (DID) approach. The results exhibit that SARS significantly reduces TFP by 3.12-5.81%, lasting for three to five years. Further, this impact is heterogeneous across industries. A significantly negative impact is found in labor intensive industries, while capital and technology intensive industries is less affected. Contrarily, a significantly positive impact is observed in those industries necessary for life and production. Mechanism tests show that the impact on TFP is caused by a reduction in labour productivity and a decrease in innovation investment after SARS outbreak. This study highlights the importance of more targeted policy on Covid-19 and similar epidemics both in industrial, national and international level.
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http://dx.doi.org/10.1016/j.asieco.2022.101507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9251906PMC
October 2022

Genome-Wide Association Analysis and Gene Mining of Resistance to China Race 1 of Frogeye Leaf Spot in Soybean.

Front Plant Sci 2022 22;13:867713. Epub 2022 Jun 22.

Key Laboratory of Soybean Biology in Chinese Ministry of Education, Key Laboratory of Soybean Biology and Breeding, Genetics of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin, China.

Soybean frogeye leaf spot (FLS) is a worldwide fungal disease. Its higher occurrence frequency and wider distribution range always led to severe yield losses of soybean, therefore, breeding new cultivars with FLS resistance has been an important breeding goal for soybean breeders. In this study, an association panel of 183 representative soybean accessions was used to evaluate their resistance to FLS race 1, and to identify quantitative trait nucleotides (QTNs) and candidate genes based on genome-wide association study (GWAS) and high-throughput single-nucleotide polymorphisms (SNPs). A total of 23,156 high-quality SNPs were developed using the specific locus-amplified fragment sequencing (SLAF-seq) approach. Finally, 13 novel association signals associated with FLS race 1 resistance were identified by the compressed mixed linear model (CMLM). In addition, 119 candidate genes were found within the 200-kb flanking genomic region of these 13 peak SNPs. Based on the gene-based association analysis, haplotype analysis, expression pattern analysis, and virus-induced gene silencing (VIGS) systems, four genes (, , , and ) were preliminarily proved to play an important role in the soybean resistance to FLS race 1.
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http://dx.doi.org/10.3389/fpls.2022.867713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9257224PMC
June 2022

Acute sleep deprivation increases inflammation and aggravates heart failure after myocardial infarction.

J Sleep Res 2022 Jul 3:e13679. Epub 2022 Jul 3.

Department of Anesthesiology, Xiangya Hospital Central South University, Changsha, China.

Sleep disorders have been observed among patients with heart failure. The aim of this study was to investigate whether acute sleep deprivation (SD) aggravates left heart function. Male C57B/L6 mice were assigned to four experimental groups. Ligation of the left anterior descending branch (LAD) caused myocardial infarction (MI) in mice in the LAD group and the LAD+SD group, while mice in the sham and sham+SD groups underwent the same surgery without ligation. Echocardiography was performed before and 8 weeks after ligation of the LAD to evaluate the left ventricular internal diameter at diastole (LVIDd), left ventricular internal diameter at systole (LVIDs), ejection fraction (EF), and fractional shortening (FS). Seven days of sleep deprivation induced using the modified single platform method resulted in a lower EF and FS and a higher LVIDd and LVIDs, as well as increased expression of the IL-1β, IL-18, and IL-10 mRNAs in the left ventricular tissue of MI mice. ELISA also indicated higher levels of IL-1β and IL-10 in the LAD+SD group. It was concluded that acute sleep deprivation induced cardiovascular alterations in cardiac structure and function in HF mice, accompanied by increased levels of inflammatory cytokines.
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http://dx.doi.org/10.1111/jsr.13679DOI Listing
July 2022

Protective Effects of Polydatin on Reproductive Injury Induced by Ionizing Radiation.

Dose Response 2022 Apr-Jun;20(2):15593258221107511. Epub 2022 Jun 26.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China.

The reproductive system is vulnerable to ionizing radiation, which is a hot research topic at present. We tested the effect of polydatin on spermatocytes(GC-1 cells) after X-ray irradiation. The reproductive damage model of C.elegans was established by 60Coγ-ray, and the protective effect of polydatin on reproductive damage caused by ionizing radiation was evaluated. We quantified the ROS levels of GC-1 cells and C.elegans after irradiation with polydatin and evaluated the anti-apoptosis effect of polydatin at proper concentration. Differential genes of C.elegans reproductive damage were screened out from transcriptome sequencing results and comparable GEO datasets. It was proved that 100μM polydatin significantly reduced the apoptosis of GC-1 cells induced by 2 Gy X-ray. In addition, the longevity, reproductive capacity, germ cell apoptosis and spawning and hatching capacity of polydatin were tested. The results showed that 100 μM polydatin content significantly increased the influence of 50 Gy 60Coγ-ray on reproductive capacity of C.elegans. Quantitative analysis of mRNA and protein levels of apoptosis-related genes and reproductive-related genes by qRT-PCR and Western blotcon firmed that polydatin with appropriate dosage had good protective effects on reproductive damage caused by radiation, which laid a foundation for the application research of polydatin in radiation protection.
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http://dx.doi.org/10.1177/15593258221107511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9244944PMC
June 2022

Knowledge Mapping of Olfactory Dysfunction: A Bibliometric Study.

Front Syst Neurosci 2022 13;16:904982. Epub 2022 Jun 13.

Department of Otorhinolaryngology Head and Neck Surgery, Children's Hospital, Capital Institute of Pediatrics, Beijing, China.

Background: Olfaction is one of the five basic senses of human beings. As such, olfactory dysfunction seriously affects patients' quality of life and can even endanger them. In recent years, olfactory dysfunction has attracted greater research interest, and numerous studies have been published on olfactory dysfunction. However, there are few studies on olfactory dysfunction through bibliometric analysis. This study aims to describe the current situation and identify the foci and potential new research directions of olfactory dysfunction using a bibliometric approach.

Methods: Articles related to olfactory dysfunction published from 2002 to 2021 were located in the Web of Science Core Collection of Clarivate Analytics (London, UK). Bibliometric analyses were conducted with the CiteSpace (Chaomei Chen, Drexel University, Philadelphia, PA, USA) and VOSviewer (Center for Science and Technology Studies, Leiden University, Leiden, Netherlands) software programs.

Results: The number of articles published each year showed an upward trend, especially in 2020, where a sharp increase had occurred due to the coronavirus disease 2019 (COVID-19) pandemic. The United States was the country with the most publications and the strongest international cooperation. In terms of institutions, the greatest number of publications from a single institution came from Dresden University of Technology. Thomas Hummel was the author who had contributed the most articles. An analysis of co-citation networks and burst keywords in the field revealed a shift from "gonadotropin-releasing hormone" and "apoptosis" earlier on to "olfactory training," "COVID-19," and "Parkinson's disease" more recently. "Outcome," "COVID-19," "infection," and "pathogenesis" are topics of the research frontier and hotspots.

Conclusion: More attention has been paid to olfactory dysfunction as the understanding of it has improved in the past 20 years. This study provides researchers with an objective, systematic, and comprehensive analysis of the literature on olfactory dysfunction. The current frontier areas and hotspots in the field focus on the pathological mechanisms of olfactory dysfunction after infection with COVID-19 and its different prognoses. The pathophysiological mechanism of olfactory dysfunction in neurodegenerative diseases and COVID-19 will be a primary future research direction.
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http://dx.doi.org/10.3389/fnsys.2022.904982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234575PMC
June 2022

TPGS-based and S-thanatin functionalized nanorods for overcoming drug resistance in Klebsiella pneumonia.

Nat Commun 2022 06 29;13(1):3731. Epub 2022 Jun 29.

Department of Clinical Pharmacy, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, China.

Tigecycline is regarded as the last line of defense to combat multidrug-resistant Klebsiella pneumoniae. However, increasing utilization has led to rising drug resistance and treatment failure. Here, we design a D-alpha tocopheryl polyethylene glycol succinate-modified and S-thanatin peptide-functionalized nanorods based on calcium phosphate nanoparticles for tigecycline delivery and pneumonia therapy caused by tigecycline-resistant Klebsiella pneumoniae. After incubation with bacteria, the fabricated nanorods can enhance tigecycline accumulation in bacteria via the inhibitory effect on efflux pumps exerted by D-alpha tocopheryl polyethylene glycol succinate and the targeting capacity of S-thanatin to bacteria. The synergistic antibacterial capacity between S-thanatin and tigecycline further enhances the antibacterial activity of nanorods, thus overcoming the tigecycline resistance of Klebsiella pneumoniae. After intravenous injection, nanorods significantly reduces the counts of white blood cells and neutrophils, decreases bacterial colonies, and ameliorates neutrophil infiltration events, thereby largely increasing the survival rate of mice with pneumonia. These findings may provide a therapeutic strategy for infections caused by drug-resistant bacteria.
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http://dx.doi.org/10.1038/s41467-022-31500-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243133PMC
June 2022

Theory-Driven Design of a Cationic Accelerator for High-Performance Electrolytic MnO -Zn Batteries.

Adv Mater 2022 Jun 29:e2203249. Epub 2022 Jun 29.

Department of Applied Chemistry, School of Chemistry and Materials Science, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui, 230026, China.

Aqueous electrolytic MnO -Zn batteries are considered as one of the most promising energy-storage devices for their cost effectiveness, high output voltage, and safety, but their electrochemical performance is limited by the sluggish kinetics of cathodic MnO /Mn and anodic Zn/Zn reactions. To overcome this critical challenge, herein, a cationic accelerator (CA) strategy is proposed based on the prediction of first-principles calculations. Poly(vinylpyrrolidone) is utilized as a model to testify the rational design of the CA strategy. It manifests that the CA effectively facilitates rapid cations migration in electrolyte and adequate charge transfer at electrode-electrolyte interface, benefiting the deposition/dissolution processes of both Mn and Zn cations to simultaneously improve kinetics of cathodic MnO /Mn and anodic Zn/Zn reactions. The resulting MnO -Zn battery regulated by CA exhibits large reversible capacities of 455 mAh g and 3.64 mAh cm at 20 C, as well as a long lifespan of 2000 cycles with energy density retention of 90%, achieving one of the best overall performances in the electrolytic MnO -Zn batteries. This comprehensive work integrating theoretical prediction with experimental studies provides opportunities to the development of high-performance energy-storage devices.
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http://dx.doi.org/10.1002/adma.202203249DOI Listing
June 2022

The "Hand as Foot" teaching method in salivary gland imaging of nuclear medicine.

Asian J Surg 2022 Jun 20. Epub 2022 Jun 20.

Department of Nuclear Medicine, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010050, China; Inner Mongolia Key Laboratory of Molecular Imaging, Hohhot, 010050, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.06.060DOI Listing
June 2022

Synthesis and Spectral Properties of Aggregation-Induced Emission-Active Push-Pull Chromophores Based On Isoindole Scaffolds.

Org Lett 2022 07 22;24(25):4557-4562. Epub 2022 Jun 22.

The Key Laboratory of Functional Molecular Solids, Ministry of Education, School of Chemistry and Materials Science, Anhui Normal University, Wuhu, Anhui 241002, China.

A new class of tailor-made push-pull isoindole fluorophores has been synthesized through the combination of Suzuki coupling and Knoevenagel reactions. The efficient synthetic strategy rendered the isoindole scaffold as the π-bridge and the isolation spacer and provided dyes bearing various types of electron donors and electron acceptors for manipulating their energy gaps and tuning their absorptions and emissions. Most of the -alkylated isoindole dyes showed aggregation-induced emission behaviors suitable for bioimaging and nice solid-state emission with maxima up to 851 nm.
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http://dx.doi.org/10.1021/acs.orglett.2c01659DOI Listing
July 2022

Case Report: Multimodal Imaging Guides the Management of an Eosinophilic Leukemia Patient With Eosinophilic Myocarditis and Intracardiac Thrombus.

Front Cardiovasc Med 2022 3;9:903323. Epub 2022 Jun 3.

Department of Cardiology, First Affiliated Hospital of Dalian Medical University, Dalian, China.

Background: Eosinophilic leukemia (EL) is a rare, serious and potentially life-threatening condition characterized by the overproduction of eosinophils leading to tissue eosinophilic infiltration and damage. Although multiple organ systems may be involved, progressive eosinophilic myocarditis (EM) is the most common cause of morbidity and mortality. Early diagnosis and follow-up surveillance combined with multimodal imaging are crucial for appropriate treatment of EM.

Case Summary: It's a rare case of EL with EM and intracardiac thrombus in a 59-year-old patient who presented with asthenia for 3 weeks. Full blood count analysis indicated significant eosinophilia. Bone marrow aspirate revealed dysplastic eosinophilia and a FIP1L1-PDGFRA fusion gene (4q12) was detected, confirming EL. Echocardiography revealed EM with intracardiac thrombus. This was later confirmed by cardiac magnetic resonance imaging. The patient was commenced on imatinib and prednisolone and good clinical response was obtained. Through 18F-FAPI PET/CT imaging, we obtained visualization of fibroblast activation changes in the early stage of cardiac structure remodeling. With anti-fibrotic therapy after heart failure, the patient achieved a good clinical response.

Conclusion: This case demonstrates visualization of fibroblast activation after EM. Multimodality imaging can provide early diagnosis and may guide tailored antifibrotic therapy in early stage of EM.
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http://dx.doi.org/10.3389/fcvm.2022.903323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204136PMC
June 2022

Meta-Analysis of the Correlation between Postoperative Cognitive Dysfunction and Intraoperative Cerebral Oxygen Saturation.

Authors:
Na Chen Jing Lu

Comput Math Methods Med 2022 1;2022:3731959. Epub 2022 Jun 1.

Department of Anesthesiology, Linyi People's Hospital, China.

Objective: This study is aimed at performing a meta-analysis for discussing the association between postoperative cognitive dysfunction (POCD) and cerebral cortical oxygen saturation after surgery (rSO2).

Method: Search common English databases such as Cochrane Library, PubMed, and Embase databases to evaluate the quality of all references. According to the normalized mean difference (SMD) and 95% confidence interval calculated by the revman5 software, the correlation between reported POCD and rSO2 was evaluated. The retrieval time is up to February 1, 2021.

Results: A total of 7 randomized controlled trials and 564 POCD patients were included in the study, with follow-up duration of 1-12 months. All patients were divided into control and operation subgroups. In the subgroup analysis of elderly patients, abdominal surgery, and orthopedic surgery, the mean intraoperative cerebral oxygen saturation of patients with POCD was significantly lower than those of patients without POCD ( = 55%, SMD = -0.57).

Conclusion: Lower intraoperativersO2 was associated with reduced incidence of neurological complications and renal alure as well as the length of stay in the intensive care unit and the total hospital stay.
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http://dx.doi.org/10.1155/2022/3731959DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9177326PMC
June 2022

Relationships between autistic traits, taste preference, taste perception, and eating behaviour.

Eur Eat Disord Rev 2022 Sep 12;30(5):628-640. Epub 2022 Jun 12.

Department of Rehabilitation for Brain Functions, Research Institute of National Rehabilitation Center for Persons with Disabilities, Tokorozawa, Japan.

Individuals with autism spectrum disorder exhibit atypical taste perception and eating behaviours. However, little is known about the effect of autistic traits on eating behaviours in the general population. This study explored the relationships between autistic traits, taste preferences, taste perceptions, and eating behaviours among Japanese population using an online questionnaire survey. The results showed significant effect of autistic traits on eating behaviours, that people with higher autistic traits tended to have higher selective eating behaviours, such as increased sensitivity to food texture and mixed flavours. Moreover, selective eating behaviours were correlated with the preference for sour taste and aftertaste sensitivity. Those results suggest that eating behaviours can be influenced by the relationship between autistic traits, taste perceptions, and taste preferences. We discuss these results in the context of previous findings, and future investigations into the possibility of solving selective eating problems in individuals with autism.
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http://dx.doi.org/10.1002/erv.2931DOI Listing
September 2022

The Triaxial Test of Polypropylene Fiber Reinforced Fly Ash Soil.

Materials (Basel) 2022 May 26;15(11). Epub 2022 May 26.

School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068, China.

Recently, soil reinforcement using arranged or randomly distributed fibers has attracted increasing attention in geotechnical engineering. In this study, polypropylene (PP) fibers with three lengths (6, 12, and 24 mm) and three mass percentages (0.5%, 1%, and 1.5%) were used to reinforce a coal fly ash soil (FAS) mixture. Unconsolidated, undrained triaxial tests were carried out in order to study the mechanical properties of the polypropylene fiber-reinforced FAS mixture and evaluate the impact of fiber on the shear strength of the FAS mixture. It is found that the fiber length of 12 mm could significantly improve the shear strength of the polypropylene fiber reinforced FAS mixture, and little effect is shown on the shear strength while using a fiber length of 24 mm. Additional fibers enhance the energy absorption capacity of the FAS specimens and therefore the highest energy absorption capacity occurs when the fiber content is 1% and the fiber length is 12 mm. The peak deviator stress enhances impressively with the addition of polypropylene fiber. The impact of fiber on the peak deviator stress is the largest when fiber content is within 1.0%. The fiber length has little effect on the peak deviator stress when it exceeds 12 mm.
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http://dx.doi.org/10.3390/ma15113807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9180948PMC
May 2022

Mechanism Study on Radiosensitization Effect of Curcumin in Bladder Cancer Cells Regulated by Filamin A.

Dose Response 2022 Apr-Jun;20(2):15593258221100997. Epub 2022 Jun 2.

State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Soochow University, Suzhou, China.

Objective: To study the radiosensitization effect of curcumin, a natural product with anti-inflammatory and anti-cancer properties, in bladder cancer cells and identify the specific role of FLNA gene in that process.

Methods: CCK-8 method was initially adopted to identify the proper interventional concentration of curcumin. T24 bladder cancer cells were subjected to CCK-8, flow cytometry, and colony formation assay to study the cell biological behaviors under different interventions. γ-H2AX test was performed to test the level of damage in T24 cells. RT-qPCR and Western blot were conducted to measure FLNA mRNA and protein levels.

Results: Low-dose curcumin (10, 20 μM) following X-ray exposure resulted in increased DNA damage, augmented apoptosis, and reduced proliferation of T24 cells. Certain radiosensitization was demonstrated when curcumin was applied at 10 μM. Additionally, elevation of FLNA gene and protein levels was also indicated upon combination treatment.

Conclusion: Low-dose curcumin has certain radiosensitization effect in bladder cancer, where FLNA plays a certain regulatory role.
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http://dx.doi.org/10.1177/15593258221100997DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168873PMC
June 2022

Psychological Nursing Effect of Patients with Gynecological Malignant Tumor.

Biomed Res Int 2022 23;2022:1569656. Epub 2022 May 23.

The First People's Hospital of Lianyungang, 222001, China.

Objective: To analyze the psychological nursing effect of patients with gynecological malignant tumor.

Method: A total of 104 patients with gynecological malignant tumor receiving chemotherapy in our hospital from December 2019 to November 2020 were selected and randomly divided into observation group and control group with 52 cases each. Patients in the control group were treated with routine nursing of gynecological malignant tumor chemotherapy. The observation group applied psychological nursing methods on the basis of the control group, and psychological status, quality of sleep, quality of life, treatment coordination, and adverse reactions were compared between the two groups.

Results: There was no difference in anxiety and depression scores ( > 0.05), the postintervention score was lower than the preintervention score, and the reduction was more significant in the observation group ( < 0.05). After intervention, PSQI scores of the two groups of subjects were significantly lower than before intervention ( < 0.05), subjects in the observation group were significantly lower than those in the control group, and the difference was statistically significant ( < 0.05). After intervention, the total score of FACT-B scale was significantly higher in the observation group than in the control group, and the difference was statistically significant ( < 0.05). After the implementation of psychological nursing, the degree of tumor treatment cooperation of observation group was higher than the control group ( < 0.05). After intervention, there was no statistically significant difference in hematology, allergic reaction, or hair loss between the two groups ( > 0.05). The incidence of gastrointestinal and neurological adverse events in the observation group was significantly lower than that in the control group, and the difference was statistically significant ( < 0.05).

Conclusion: The psychological state of patients with gynecological malignant tumor mainly included anxiety and terror and so on. Through psychological counseling, it could effectively alleviate the abovementioned bad psychology, improve the cooperation of patients with tumor treatment, and reduce the occurrence of patients' adverse reactions.
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http://dx.doi.org/10.1155/2022/1569656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9152411PMC
June 2022

Zero-valent iron coupled calcium hydroxide: A highly efficient strategy for removal and magnetic separation of concentrated fluoride from acidic wastewater.

Sci Total Environ 2022 Sep 31;838(Pt 3):156336. Epub 2022 May 31.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Institute of Environmental & Applied Chemistry, College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China.

The removal of concentrated fluoride in acidic wastewater by the conventional Ca(OH) method is challenged by the insufficient efficiency and difficult separation of fine CaF precipitate. Herein, we construct a strategy to tackle these challenges by coupling zero-valent iron (ZVI) with Ca(OH). ZVI reduces fluoride concentration from 12,000 to 3980 mg L under optimal conditions primarily through the in-situ growth of porous FeF·4HO shell on its surface, which simultaneously assists fluoride removal via adsorption. The residual fluoride after ZVI treatment then decreases to 6.74 mg L via precipitation with Ca(OH). Interestingly, the iron ions dissolved from ZVI also participate in the precipitation to form magnetite. This co-precipitation reinforces the fluoride removal and meanwhile endows the resulted precipitates with magnetism, thus enabling the perfect solid-liquid separation by the magnetic field before discharge. The application prospect of this coupling strategy is further verified by its ability in decreasing the concentrations of fluoride and other coexisting heavy metals (Zn, Cd and Pb) in real smeltery wastewater below their discharge limitations. This study provides a promising strategy for the treatment of concentrated fluoride in acidic wastewater and also highlights ZVI as a good candidate to couple with conventional methods for enhanced pollution control.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156336DOI Listing
September 2022

Solar-Driven Overproduction of Biofuels in Microorganisms.

Angew Chem Int Ed Engl 2022 08 24;61(32):e202207132. Epub 2022 Jun 24.

College of Chemistry and Molecular Sciences, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.

Microbial cell factories reinvigorate current industries by producing complex fine chemicals at low costs. Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is the main reducing power to drive the biosynthetic pathways in microorganisms. However, insufficient intrinsic NADPH limits the productivity of microorganisms. Here, we report that supplying microorganisms with long-lived electrons from persistent phosphor mesoporous Al O (meso-Al O ) can elevate the NADPH level to facilitate efficient fine chemical production. The defects in meso-Al O were demonstrated to be highly efficient in prolonging electrons' lifetime. The long-lived electrons in meso-Al O can pass the material-microorganism interface and power the biosynthetic pathways of E. coli to produce jet fuel farnesene. This work represents a reliable strategy to design photo-biosynthesis systems to improve the productivity of microorganisms with solar energy.
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http://dx.doi.org/10.1002/anie.202207132DOI Listing
August 2022

Non-enzymatic signal amplification-powered point-of-care SERS sensor for rapid and ultra-sensitive assay of SARS-CoV-2 RNA.

Biosens Bioelectron 2022 Sep 16;212:114379. Epub 2022 May 16.

State Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023, China. Electronic address:

The development of rapid and ultra-sensitive detection technology of SARS-CoV-2 RNA for shortening the diagnostic window and achieving early detection of virus infections is a huge challenge to the efficient prevention and control of COVID-19. Herein, a novel ultra-sensitive surface-enhanced Raman spectroscopy (SERS) sensor powered by non-enzymatic signal amplification is proposed for rapid and reliable assay of SARS-CoV-2 RNA based on SERS-active silver nanorods (AgNRs) sensing chips and a specially designed smart unlocking-mediated target recycling signal amplification strategy. The SERS sensing was carried out by a one-pot hybridization of the lock probes (LPs), hairpin DNAs and SERS tags with SARS-CoV-2 RNA samples on an arrayed SERS sensing chip to achieve the recognition of SARS-CoV-2 RNA, the execution of nuclease-free unlocking-mediated target recycling signal amplification, and the combination of SERS tags to generate SERS signal. The SERS sensor for SARS-CoV-2 RNA can be achieved within 50 min with an ultra-high sensitivity low to 51.38 copies/mL, and has good selectivity in discriminating SARS-CoV-2 RNA against other respiratory viruses in representative clinical samples, which is well adapted for rapid, ultra-sensitive, multi-channel and point-of-care testing of viral nucleic acids, and is expected to achieve detection of SARS-CoV-2 infection in earlier detection windows for efficient COVID-19 prevention and control.
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http://dx.doi.org/10.1016/j.bios.2022.114379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110061PMC
September 2022

A specific upregulated long noncoding RNA in colorectal cancer promotes cancer progression.

JCI Insight 2022 Aug 8;7(15). Epub 2022 Aug 8.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan, China.

Long noncoding RNA (lncRNA) plays a crucial role in the pathogenesis of various diseases, including colorectal cancer (CRC). The gene mutations of adenomatous polyposis coli (APC) were found in most patients with CRC. They function as important inducers of tumorigenesis. Based on our microarray results, we identified a specific upregulated lncRNA in CRC (SURC). Further analysis showed that high SURC expression correlated with poorer disease-free survival and overall survival in patients with CRC. Furthermore, we found that mutated APC genes can promote the transcription of SURC by reducing the degradation of β-catenin protein in CRC. Functional assays revealed that knockdown of SURC impaired CRC cell proliferation, colony formation, cell cycle, and tumor growth. Additionally, SURC promotes CCND2 expression by inhibiting the expression of miR-185-5p in CRC cells. In conclusion, we demonstrate that SURC is a specific upregulated lncRNA in CRC and the SURC/miR-185-5p/CCND2 axis may be targetable for CRC diagnosis and therapy.
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http://dx.doi.org/10.1172/jci.insight.158855DOI Listing
August 2022

Myomatous erythrocytosis syndrome: A case report.

World J Clin Cases 2022 Apr;10(10):3206-3212

Department of Obstetrics and Gynaecology, Peking University First Hospital, Beijing 100034, China.

Background: Uterine myoma is the most common benign tumor among women and is often accompanied by anemia. Here, we report the case of a patient with a very large leiomyoma but with a hemoglobin level as high as 197 g/L. After undergoing hysterectomy, all her hematological parameters returned to normal. Immunohistochemical staining of her myoma for erythropoietin showed strong positivity, which suggested that erythropoietin may be the cause of her erythrocytosis. A multidisciplinary team played a significant role in treating the disease.

Case Summary: A 47-year-old woman visited our department complaining that her abdomen had been continuously growing for the past 2 years. After careful examinations, she was suspected of having a very large leiomyoma. She was also diagnosed with erythrocytosis because her RBC count was 6.49 × 10/L, hemoglobin was 197 g/L. Following a multidisciplinary team consultation, bilateral ureteral stents were placed, and 800 mL blood was removed by phlebotomy. The patient then underwent hysterectomy and bilateral salpingectomy. She recovered well from the operation, and her hemoglobin level decreased sharply following the surgery. Low-molecular-weight heparin was administered daily to prevent postoperative thrombosis. She was discharged from the hospital on the fourth postoperative day. Two months later, all her hematological parameters returned to normal. Pathological analysis of the myoma revealed that it was a benign leiomyoma, with partial hyalinization, and strong positivity for erythropoietin in immunohistochemical staining suggested that erythropoietin may be responsible for the erythrocytosis.

Conclusion: Erythropoietin ectopically produced from the myoma was responsible for the erythrocytosis in this patient. A multidisciplinary team is strongly recommended.
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http://dx.doi.org/10.12998/wjcc.v10.i10.3206DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082685PMC
April 2022

Impact of Pain, Agitation, and Delirium Bundle on Delirium and Cognitive Function.

J Nurs Res 2022 Aug 1;30(4):e222. Epub 2022 Aug 1.

MSN, RN, Director, Nursing Department, Tianjin Third Central Hospital, Tianjin, People Republic of China.

Background: Differences in short-term cognitive function between mechanically ventilated patients treated with multicomponent interventions and those receiving routine nursing care have not been established because of the lack of follow-up in previous studies.

Purpose: This study was designed to evaluate the effects of the pain, agitation, and delirium (PAD) care bundle on delirium occurrence and clinical outcomes, specifically in terms of short-term cognitive function, in mechanically ventilated patients.

Methods: Data on 243 patients with mechanical ventilation were analyzed from January 2017 to February 2019. The eligible patients were divided randomly into two groups. The control group ( n = 120) received usual care, whereas the intervention group ( n = 123) received the PAD bundle, including pain monitoring and management, light sedation and daily awakening, early mobility, sleep promotion, and delirium monitoring. The incidence and duration of delirium, ventilator time, and intensive care unit (ICU) length of stay were compared between the two groups. Upon discharge from the ICU and at 3 and 6 months after discharge, cognitive function was assessed using the Montreal Cognitive Assessment scale and compared between the two groups.

Results: The incidence of delirium was reduced significantly in the intervention group, and significant decreases in the duration of delirium, ventilator time, and ICU length of stay were found. Cognitive impairment in the intervention group was significantly lower at the 3-month follow-up assessment.

Conclusions/implications For Practice: The PAD bundle was shown to be associated with a lower incidence of delirium and improved clinical outcomes. Short-term cognitive impairment occurred in fewer patients who were managed with the PAD bundle after ICU discharge. Our findings indicate that the PAD bundle has the potential to improve clinical outcomes. The administrative staff of ICUs should use strategies, such as interdisciplinary teamwork, to facilitate the buy-in and implementation of interventions.
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http://dx.doi.org/10.1097/jnr.0000000000000497DOI Listing
August 2022
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