Publications by authors named "N N Lebedeva"

394 Publications

'Candidatus Gromoviella agglomerans', a novel intracellular Holosporaceae parasite of the ciliate Paramecium showing marked genome reduction.

Environ Microbiol Rep 2021 Nov 12. Epub 2021 Nov 12.

Dipartimento di Biologia, Università di Pisa, Pisa, Italy.

Holosporales are an alphaproteobacterial lineage encompassing bacteria obligatorily associated with multiple diverse eukaryotes. For most representatives, little is known on the interactions with their hosts. In this study, we characterized a novel Holosporales symbiont of the ciliate Paramecium polycaryum. This bacterium inhabits the host cytoplasm, frequently forming quite large aggregates. Possibly due to such aggregates, host cells sometimes displayed lethal division defects. The symbiont was also able to experimentally stably infect another Paramecium polycaryum strain. The bacterium is phylogenetically related with symbionts of other ciliates and diplonemids, forming a putatively fast-evolving clade within the family Holosporaceae. Similarly to many close relatives, it presents a very small genome (<600 kbp), and, accordingly, a limited predicted metabolism, implying a heavy dependence on Paramecium, thanks also to some specialized membrane transporters. Characterized features, including the presence of specific secretion systems, are overall suggestive of a mild parasitic effect on the host. From an evolutionary perspective, a potential ancestral trend towards pronounced genome reduction and possibly linked to parasitism could be inferred, at least among fast-evolving Holosporaceae, with some lineage-specific traits. Interestingly, similar convergent features could be observed in other host-associated lineages, in particular Rickettsiales among Alphaproteobacteria.
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http://dx.doi.org/10.1111/1758-2229.13021DOI Listing
November 2021

Theoretical and experimental study of interaction of macroheterocyclic compounds with ORF3a of SARS-CoV-2.

Sci Rep 2021 09 30;11(1):19481. Epub 2021 Sep 30.

G.A. Krestov Institute of Solution Chemistry of the Russian Academy of Sciences, 153045, Ivanovo, Russia.

The pandemic infectious disease (Covid-19) caused by the coronavirus (SARS-CoV2) is spreading rapidly around the world. Covid-19 does an irreparable harm to the health and life of people. It also has a negative financial impact on the economies of most countries of the world. In this regard, the issue of creating drugs aimed at combating this disease is especially acute. In this work, molecular docking was used to study the docking of 23 compounds with QRF3a SARS-CoV2. The performed in silico modeling made it possible to identify leading compounds capable of exerting a potential inhibitory and virucidal effect. The leading compounds include chlorin (a drug used in PDT), iron(III)protoporphyrin (endogenous porphyrin), and tetraanthraquinone porphyrazine (an exogenous substance). Having taken into consideration the localization of ligands in the QRF3a SARS-CoV2, we have made an assumption about their influence on the pathogenesis of Covid-19. The interaction of chlorin, iron(III)protoporphyrin and protoporphyrin with the viral protein ORF3a were studied by fluorescence and UV-Vis spectroscopy. The obtained experimental results confirm the data of molecular docking. The results showed that a viral protein binds to endogenous porphyrins and chlorins, moreover, chlorin is a competitive ligand for endogenous porphyrins. Chlorin should be considered as a promising drug for repurposing.
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http://dx.doi.org/10.1038/s41598-021-99072-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8484456PMC
September 2021

[Clinical and anamnestic characteristics of patients with an implanted cardioverter-defibrillator in real clinical practice (data from the Kuzbass register)].

Kardiologiia 2021 Aug 31;61(8):40-47. Epub 2021 Aug 31.

Federal State Budgetary Institution "Research Institute for Complex Issues of Cardiovascular Diseases", Sosnoviy blvd, 6, Kemerovo, 650002, Russian Federation.

Aim      To study the consistency of the practice of management, selection and routing of patients at high risk of sudden cardiac death (SCD) selected for cardioverter-defibrillator implantation (CDI) with current clinical guidelines and to evaluate the quality of subsequent outpatient follow-up and treatment based on a retrospective analysis of clinical amnestic data from the Kuzbass Registry of Patients with CDI.Material and methods  The study was based on the Registry of Patients with Implanted Cardioverter Defibrillator and included successive data of 28 patients hospitalized to the Kizbass Cardiological Center from 2015 through 2019. Social and clinical amnestic characteristics, indications for CVI, and concomitant drug therapy were analyzed retrospectively. Statistical analyses were performed with the Statistica 10.0 software (Statsoft, USA).Results Median age of patients was 59 (53; 66) years; 239 (83.6 %) men were included; 29 (10.1%) people were employed, CHI was performed in 182 (63.6 %) patients for prevention of SCC, and for secondary prevention in 104 (36.4 %) patients. 208 (72.7 %) patients were diagnosed with ischemic heart disease (IHD), and 145 (67.9 %) of them underwent myocardial revascularization. Noncoronarogenic diseases were found in 78 (27.3 %) patients, and most of them were diagnosed with dilated cardiomyopathy. All patients had chronic heart failure (CHF); half of them had stage IIA CHF. Median left ventricular ejection fraction was 30 (25; 36,5) % according to echocardiography using the Simpson method. Comorbidity was found in 151 (52.8 %) patients. 128 (44.8%) patients received a triple neurohormonal blockade for CHF treatment; titration to target doses was not performed in any of them. Antiarrhythmics were administered to 150 (52.4 %) patients.Conclusion      According to the data from the Kuzbass Registry of CVI, the main patient cohort consisted of men of pension age with IHD and CHF. Before CVI, more than a half of them had not received an optimum drug therapy and not all of them had received target lesion revascularization. Creating and analysis of Registries of CHI patients is an effective method for identifying existing problems in patient management before CVI and for optimizing their subsequent follow-up and treatment.
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http://dx.doi.org/10.18087/cardio.2021.8.n1651DOI Listing
August 2021

Data on bulk rock compositions, geochemical and textural contrasts between central and marginal parts of dykes, and MELTS modeling of lamprophyre dykes in the Kola Alkaline Carbonatite Province (N Europe).

Data Brief 2021 Oct 19;38:107307. Epub 2021 Aug 19.

Geological Department, Lomonosov Moscow State University, GSP-1, Leninskie Gory, Moscow, Russia.

This publication is a series of datasets that accompany a manuscript on petrology of lamprophyre dykes in the Kola Alkaline Carbonatite Province (N Europe) [1]. The datasets served as the basis for interpretation of melt crystallization for lamprophyric and carbonatitic dykes in the crust, to supplement many papers devoted to mantle sources and melting parameters of these rocks based on radiogenic isotopes and trace elements. The first dataset contains bulk major and trace element compositions of the dykes in three areas, Kandalaksha, Kandaguba and Turiy Mys, along with supplementary information on sampling locations and dyke characteristics. The second dataset represents photos, major and trace element plots illustrating extreme mineralogical and textural heterogeneity and layering observed in some dykes. The photographs include field photographs, optical and electron microscopy shots for thin sections in central and marginal parts of the dykes. They should be viewed together with Harker and REE diagrams illustrating the changes from dyke margins to dyke cores. The third and fourth datasets are output tables from Ryolite-MELTS software used to model crystal fractionation of the dykes from the parental melts and various P, T, fO, XCO, XHO parameters. One table shows compositions of evolved melts, while the other lists crystallizing phases.
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http://dx.doi.org/10.1016/j.dib.2021.107307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8390559PMC
October 2021

Destruction of Chitosan and Its Complexes with Cobalt(II) and Copper(II) Tetrasulphophthalocyanines.

Polymers (Basel) 2021 Aug 19;13(16). Epub 2021 Aug 19.

G.A. Krestov Institute of Solution Chemistry of the Russian Academy of Sciences, 153045 Ivanovo, Russia.

Chitosan is a naturally occurring polysaccharide derived from chitin with a wide range of uses. Phthalocyanines are macroheterocyclic compounds that have a number of useful properties such as coloring and catalytic and antioxidant activity. Phthalocyanines are able to immobilize on chitosan, forming complexes with new useful properties. In this work, we evaluated the ability of phthalocyanines to increase the thermal stability of chitosan. Chitosan (CS) forms complexes with copper(II)-(CuPc) and cobalt(II)-(CoPc) tetrasulphophthalocyanines. The processes of destruction of chitosan (CS) and its complexes with sulphophthalocyanines CuPc and CoPc in oxidizing and inert atmospheres have been studied. It was established that, regardless of the atmosphere composition, the first chemical reactions taking place in the studied systems are elimination reactions. The latter ones in the case of chitosan and complex CS-CuPc lead to the formation of spatially crosslinked polymer structures, and it causes the release of CuPc from the polymer complex. It was found that in the case of CS-CoPc elimination reactions did not lead to the formation of crosslinked polymer structures but caused the destruction of the pyranose rings with a partial release of CoPc. Metallophthalocyanines showed antioxidant properties in the composition of complexes with chitosan, increasing the temperature of the beginning of glycosidic bond cleavage reaction by 30-35 °C in comparison with the similar characteristics for chitosan.
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http://dx.doi.org/10.3390/polym13162781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400729PMC
August 2021
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