Publications by authors named "N M Bazhin"

22 Publications

The murburn precepts for cellular ionic homeostasis and electrophysiology.

J Cell Physiol 2021 Aug 11. Epub 2021 Aug 11.

Department of Mechanical Engineering, Gifu University, Yanagido, Gifu, Japan.

Starting from the basic molecular structure and redox properties of its components, we build a macroscopic cellular electrophysiological model. We first present a murburn purview that could explain ion distribution in bulk-milieu/membrane-interface and support the origin of trans-membrane potential (TMP) in cells. In particular, the discussion focuses on how cells achieve disparity in the distribution of monovalent and divalent cations within (K  > Na  > Mg  > Ca ) and outside (Na  > K  > Ca  > Mg ). We explore how TMP could vary for resting/graded/action potentials generation and project a model for impulse conduction in neurons. Outcomes based on murburn bioenergetic equilibriums leading to solubilization of ion-pairs, membrane's permittivity, protein channels' fluxes, and proteins' innate ability to bind/adsorb ions selectively are projected as the integral rationale. We also provide experimental modalities to ratify the projections.
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http://dx.doi.org/10.1002/jcp.30547DOI Listing
August 2021

Structure-function correlations and system dynamics in oxygenic photosynthesis: classical perspectives and murburn precepts.

J Biomol Struct Dyn 2021 Jul 29:1-27. Epub 2021 Jul 29.

Satyamjayatu: The Science & Ethics Foundation, Kulappully, Kerala, India.

Highlights: Contemporary beliefs on oxygenic photosynthesis are critiqued.Murburn model is suggested as an alternative explanation.In the new model, diffusible reactive species are the main protagonists.All pigments are deemed photo-redox active in the new stochastic mechanism.NADPH synthesis occurs via simple electron transfers, not via elaborate ETC.Oxygenesis is delocalized and not just centered at Mn-Complex.Energetics of murburn proposal for photophosphorylation is provided.The proposal ushers in a paradigm shift in photosynthesis research.
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http://dx.doi.org/10.1080/07391102.2021.1953606DOI Listing
July 2021

The murburn precepts for aerobic respiration and redox homeostasis.

Prog Biophys Mol Biol 2021 Dec 10;167:104-120. Epub 2021 Jun 10.

Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, St. Institutskaya 3, 630090, Novosibirsk, Russia. Electronic address:

Murburn concept is a new perspective to metabolism which posits that certain redox enzymes/proteins mediate catalysis outside their active site, via diffusible reactive oxygen species (DROS, usually deemed as toxic wastes). We have recently questioned the proton-centric chemiosmotic rotary ATP synthesis (CRAS) explanation for mitochondrial oxidative phosphorylation (mOxPhos) and proposed an oxygen-centric murburn model in lieu. Herein, the chemical equations and thermodynamic foundations for this new model of mOxPhos are detailed. Standard transformed Gibbs free energy values of respiratory reactions are calculated to address the spontaneity, control, and efficiency of oxidative phosphorylation. Unlike the deterministic/multi-molecular and 'irreducibly complex' CRAS model, the stochastic/bimolecular and parsimonious murburn reactions afford a more viable precept for the variable and non-integral stoichiometry, higher yield for NADH than FADH, and origin/evolution of oxygen-centric cellular life. Also, we present tangible DROS-based explanations for the multiple roles of various reaction components, HCN > HS order of cellular toxicity in aerobes, and explain why oxygen inhibits anaerobes. We highlight the thermodynamic significance of proton deficiency in NADH/mitochondria and link the 'oxygen → DROS → water' metabolic pathway to the macroscopic physiologies of ATP-synthesis, trans-membrane potential, thermogenesis, and homeostasis. We also provide arguments for the extension of the murburn bioenergetics model to life under anoxic and extreme/unique habitats. In the context of mOxPhos, our findings imply that DROS should be seen as an essential requisite for life, and not merely as pathophysiological manifestations.
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http://dx.doi.org/10.1016/j.pbiomolbio.2021.05.010DOI Listing
December 2021

Standard Values of the Thermodynamic Functions of the Formation of Ions in an Aqueous Solution and their Change during Solvation.

Authors:
N M Bazhin

J Phys Chem A 2020 Dec 17;124(52):11051-11060. Epub 2020 Dec 17.

Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk 630090, Russia.

The standard thermodynamic parameters of inorganic ions in an aqueous solution are based on the use of a convention on the zero values of the thermodynamic functions of protons. However, this does not allow calculating the thermodynamic parameters of reactions in which, along with ions, organic and inorganic compounds participate, the thermodynamic parameters of which are determined in the standard system of values since frames of reference are different. In this work, we calculated the standard thermodynamic parameters of inorganic ions in an aqueous solution in the same system of values that is used for organic compounds. The calculation of the standard values of the Gibbs function, enthalpy, and entropy is based on the use of reliably determined thermodynamic parameters of protons in an aqueous solution according to Tissandier et al. [ , 1998, 102, 7787-7794]. The results allow one to calculate the thermodynamic parameters of reactions in aqueous media with the participation of inorganic ions and organic compounds. In this work, the thermodynamic parameters of solvation processes in aqueous solutions are determined. The standard entropy of ions in an aqueous solution was calculated by four independent methods.
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http://dx.doi.org/10.1021/acs.jpca.0c08737DOI Listing
December 2020

Standard and transformed values of gibbs energy formation for some radicals and ions involved in biochemical reactions.

Authors:
N M Bazhin

Arch Biochem Biophys 2020 06 5;686:108282. Epub 2020 Feb 5.

Institute of Chemical Kinetics and Combustion, Institutskaya 3, Novosibirsk, 630090 Russia, Novosibirsk State University, Pirogova 2, Novosibirsk, 630090, Russia. Electronic address:

The standard and transformed values of the Gibbs formation function of a number of radicals and ions are calculated: H, OH, HO,O, SH, NH, CH H,O,OHHO, O,SHNH, Q, NAD*, FMN, FAD. These data can be used in consideration of the thermodynamics of biochemical reactions involving free radicals.
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http://dx.doi.org/10.1016/j.abb.2020.108282DOI Listing
June 2020
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