Publications by authors named "Myung Soo Park"

89 Publications

Different patterns of belowground fungal diversity along altitudinal gradients with respect to microhabitat and guild types.

Environ Microbiol Rep 2021 Jun 23. Epub 2021 Jun 23.

School of Biological Sciences, Institute of Microbiology, Seoul National University, Seoul, South Korea.

Fungi are key components of belowground ecosystems with various ecological roles in forests. Although the changes in the richness and composition of belowground fungi across altitudinal gradients have been widely reported, only a few studies have focused on the microhabitat types along altitudinal gradients. Here, we analysed the effect of altitude on the ectomycorrhizal and non-ectomycorrhizal fungal communities in belowground microhabitats. We collected root and soil samples from 16 Pinus densiflora forests at various altitudes across Korea, and measured the soil properties as potential factors. Fungal communities were analysed by high-throughput sequencing of the internal transcribed spacer 2 (ITS2) region. We found that altitude negatively affected the species richness of root-inhabiting fungi but did not influence that of soil-inhabiting fungi. In addition, the composition of ectomycorrhizal (ECM) fungi was less influenced by altitude than non-ECM fungi. Most of the soil properties did not show a significant relationship with altitude, but the effect of soil properties was different across microhabitat types and ecological roles of fungi. Our results reveal that microhabitat types and altitudinal gradients differently affect the richness and composition of fungal communities associated with P. densiflora, providing a better understanding of plant-associated fungal communities.
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http://dx.doi.org/10.1111/1758-2229.12976DOI Listing
June 2021

In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by .

J Fungi (Basel) 2021 May 28;7(6). Epub 2021 May 28.

Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea.

In the search for antifungal agents from marine resources, we recently found that the culture filtrate of SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successively extracted with ethyl acetate and n-butanol to identify the fungicidal metabolites. Consequently, a new compound, spirosorbicillinol D (), and a new natural compound, 2',3'-dihydro-epoxysorbicillinol (), together with 11 known compounds (-), were obtained from the solvent extracts. The chemical structures were determined by spectroscopic analyses and comparison with literature values. The results of the in vitro antifungal assay showed that of the tested fungal pathogens, was the fungus most sensitive to the isolated compounds, with MIC values ranging from 6.3 to 400 µg/mL, except for trichotetronine () and trichodimerol (). When tomato plants were treated with the representative compounds (, , , and ), bisvertinolone () strongly reduced the development of tomato late blight disease compared to the untreated control. Taken together, our results revealed that the culture filtrate of SFC100166 and its metabolites could be useful sources for the development of new natural agents to control late blight caused by .
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http://dx.doi.org/10.3390/jof7060428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229967PMC
May 2021

The genus Arthrinium (Ascomycota, Sordariomycetes, Apiosporaceae) from marine habitats from Korea, with eight new species.

IMA Fungus 2021 Jun 1;12(1):13. Epub 2021 Jun 1.

Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University, Seoul, 02841, South Korea.

Species of Arthrinium are well-known plant pathogens, endophytes, or saprobes found in various terrestrial habitats. Although several species have been isolated from marine environments and their remarkable biological activities have been reported, marine Arthrinium species remain poorly understood. In this study, the diversity of this group was evaluated based on material from Korea, using morphological characterization and molecular analyses with the internal transcribed spacer (ITS) region, β-tubulin (TUB), and translation elongation factor 1-alpha (TEF). A total of 41 Arthrinium strains were isolated from eight coastal sites which represented 14 species. Eight of these are described as new to science with detailed descriptions.
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http://dx.doi.org/10.1186/s43008-021-00065-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8168325PMC
June 2021

A Biodegradable Secondary Battery and its Biodegradation Mechanism for Eco-Friendly Energy-Storage Systems.

Adv Mater 2021 Mar 2;33(10):e2004902. Epub 2021 Feb 2.

Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, 08826, Republic of Korea.

The production of rechargeable batteries is rapidly expanding, and there are going to be new challenges in the near future about how the potential environmental impact caused by the disposal of the large volume of the used batteries can be minimized. Herein, a novel strategy is proposed to address these concerns by applying biodegradable device technology. An eco-friendly and biodegradable sodium-ion secondary battery (SIB) is developed through extensive material screening followed by the synthesis of biodegradable electrodes and their seamless assembly with an unconventional biodegradable separator, electrolyte, and package. Each battery component decomposes in nature into non-toxic compounds or elements via hydrolysis and/or fungal degradation, with all of the biodegradation products naturally abundant and eco-friendly. Detailed biodegradation mechanisms and toxicity influence of each component on living organisms are determined. In addition, this new SIB delivers performance comparable to that of conventional non-degradable SIBs. The strategy and findings suggest a novel eco-friendly biodegradable paradigm for large-scale rechargeable battery systems.
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http://dx.doi.org/10.1002/adma.202004902DOI Listing
March 2021

An observational study on the effect of hypercholesterolemia developed after living donor liver transplantation on cardiac event and graft failure.

Sci Rep 2021 01 13;11(1):959. Epub 2021 Jan 13.

Department of Anesthesiology and Pain Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul, 06351, South Korea.

This study sought to evaluate the association between newly-developed significant hypercholesterolemia within one year following living donor liver transplantation (LDLT) and long term outcomes in light of cardiovascular events and graft failure. From October 2003 to July 2017, 877 LDLT recipients were stratified according to development of significant hypercholesterolemia within one year following LDLT. The primary outcome was occurrence of a major adverse cardiac event (MACE), defined as a composite of cardiac death, myocardial infarction, and coronary revascularization after LDLT. The incidence of graft failure, defined as all-cause death or retransplantation, was also compared. A total of 113 (12.9%) recipients developed significant hypercholesterolemia within one year. The differences in incidences of cardiac related events and graft related events began emerging significantly higher in the hypercholesterolemia group after 24 months and 60 months since the LDLT, respectively. After adjustment using the inverse probability of weighting, the hazard ratio (HR) for MACE was 2.77 (95% confidence interval (CI) 1.16-6.61; p = 0.02), while that for graft failure was 3.76 (95% CI 1.97-7.17, p < 0.001). A significant hypercholesterolemia after LDLT may be associated with cardiac and graft-related outcome; therefore, a further study and close monitoring of cholesterol level after LDLT is needed.
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http://dx.doi.org/10.1038/s41598-020-79673-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7806822PMC
January 2021

Taxonomic Study of the Genus (Strophariaceae, Basidiomycota) in Korea.

Mycobiology 2020 Oct 22;48(6):476-483. Epub 2020 Oct 22.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea.

The genus (Strophariaceae, Basidiomycota) is made up of wood-rotting saprotrophic mushrooms characterized by a yellow or brown pileus with scales and/or slimy, and by a brownish smooth spore with a germ pore. However, these features are not enough to distinguish its species, or separate the genus from other brown-spored wood-rotting genera such as and . Although internal transcribed spacer (ITS) sequence-based identification has improved identification accuracy for species of , most species in Korea are reported based on morphological features. To evaluate the taxonomy of species, we investigated 62 specimens collected from 1999 to 2019 in Korea using ITS sequence analysis and morphological observation. Twelve of the 16 recorded species in Korea were identified. While eight species were clearly separated, the ITS analysis did not distinguish three in the complex. Therefore, further investigation is required to distinguish these three species. ITS sequences deposited in GenBank confirm that exists in Korea. The presence of the other four species could not be confirmed through specimens or sequence information in GenBank. A taxonomic key and the ITS sequence data for Korean species are included and can be good baselines for further research on taxonomy and diversity.
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http://dx.doi.org/10.1080/12298093.2020.1831427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717605PMC
October 2020

from Rhizosphere Soil in Terrestrial and Coastal Environments in South Korea.

Mycobiology 2020 Oct 16;48(6):431-442. Epub 2020 Oct 16.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea.

, the most common genus plays an important ecological role in various terrestrial and marine environments. However, only a few species have been reported from rhizosphere soil. As part of a project to excavate Korean indigenous fungi, we investigated rhizosphere soil of six plants in the forest (terrestrial habitat) and sand dunes (coastal habitat) and focused on discovering species. A total of 64 strains were isolated and identified as 26 species in nine sections based on morphological characteristics and the sequence analysis of β-tubulin and calmodulin. Although this is a small-scale study in a limited rhizosphere soil, eight unrecorded species and four potential new species have been identified. In addition, most species from rhizosphere soil were unique to each plant. , , , , and were commonly isolated from rhizosphere soil. Eight species, , and were recorded for the first time in Korea. Here, we provide the detailed morphological description of these unrecorded species.
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http://dx.doi.org/10.1080/12298093.2020.1823611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717687PMC
October 2020

Taxonomic revision of Russula subsection Amoeninae from South Korea.

MycoKeys 2020 9;75:1-29. Epub 2020 Nov 9.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul 08826, South Korea Seoul National University Seoul South Korea.

Russula subsection Amoeninae is morphologically defined by a dry velvety pileus surface, a complete absence of cystidia with heteromorphous contents in all tissues, and spores without amyloid suprahilar spot. Thirty-four species within subsection Amoeninae have been published worldwide. Although most species in South Korea have been assigned European or North American names, recent molecular studies have shown that species from different continents are not conspecific. Therefore, the present study aims to: 1) define which species of Russula subsection Amoeninae occur on each continent using molecular phylogenetic analyses; 2) revise the taxonomy of Korean . The phylogenetic analyses using the internal transcribed spacer (ITS) and multilocus sequences showed that subsection Amoeninae is monophyletic within subgenus Heterophyllidiae section Heterophyllae. A total of 21 Russula subsection Amoeninae species were confirmed from Asia, Australia, Europe, North America, and Central America, and species from different continents formed separate clades. Three species were recognized from South Korea and were clearly separated from the European and North American species. These species are , also reported from Japan, a new species described herein, , and a new species undescribed due to insufficient material.
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http://dx.doi.org/10.3897/mycokeys.75.53673DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7669817PMC
November 2020

Successional Change of the Fungal Microbiome Pine Seedling Roots Inoculated With .

Front Microbiol 2020 25;11:574146. Epub 2020 Sep 25.

School of Biological Sciences, Institute of Microbiology, Seoul National University, Seoul, South Korea.

The pine mushroom (; Agaricales, Tricholomataceae) is an ectomycorrhizal fungus that produces a commercially valuable, edible mushrooms. Attempts to artificially cultivate has so far been unsuccessful. One method used to induce to produce fruiting bodies of in the wild is shiro (mycelial aggregations of ) transplantation. ectomycorrhization of with seedlings of has been successful, but field trials showed limited production of fruiting bodies. Few studies have been done to test what happens after transplantation in the wild, whether persists on the pine seedling roots or gets replaced by other fungi. Here, we investigated the composition and the interaction of the root fungal microbiome of seedlings inoculated with over a 3 year period after field transplantation, using high-throughput sequencing. We found a decline of colonization on pine roots and succession of mycorrhizal fungi as seedlings grew. Early on, roots were colonized by fast-growing, saprotrophic Ascomycota, then later replaced by early stage ectomycorrhiza such as . At the end, more competitive species dominated the host roots. Most of the major OTUs had negative or neutral correlation with , but several saprotrophic/plant pathogenic/mycoparasitic species in genera , , and had positive correlation with . Four keystone species were identified during succession; two species (, and ) had a positive correlation with , while the other two had a negative correlation (, ). These findings have important implications for further studies on the artificial cultivation of .
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http://dx.doi.org/10.3389/fmicb.2020.574146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7545793PMC
September 2020

Two New Species of (Agaricales, Basidiomycota) from Korea.

Mycobiology 2020 Jul 17;48(4):288-295. Epub 2020 Jul 17.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Korea.

Species of (Hydnangiaceae, Agaricales, and Basidiomycota) are well-known ectomycorrhizal symbionts of a broad range of hosts. species are characterized by brown, orange, or purple colored basidiocarps, and globose or oblong, echinulate and multinucleate basidiospores. While some species are easily identified at the species level using only the morphological characteristics, others are hard to distinguish at the species level due to small differences in morphology. Heretofore, ten species have been reported in Korea. While studying the fungal diversity in the National Parks of Korea, two new species were discovered. Species identification was done based on molecular analyses (ITS, 28S rDNA, 2, and 1), then were confirmed by their corresponding morphologies. The two newly discovered species are proposed here as and . The unique morphological characters of . that distinguish it from its closely related species are orange-brown colored basidiocarp, long basidia and the absence of cheilocystidia. is characterized by a light grayish lavender-colored pileus and the absence of cheilocystidia. Two new species are described and illustrated in the present paper.
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http://dx.doi.org/10.1080/12298093.2020.1786961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476507PMC
July 2020

New Species of (Lyophyllaceae, Basidiomycota) from Sabah (Northern Borneo), Malaysia.

Mycobiology 2020 20;48(2):95-103. Epub 2020 Mar 20.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Korea.

The genus (Lyophyllaceae, Basidiomycota) is often associated with fungus-feeding termites (Macrotermitinae) due to their strong symbiotic relationships. The genus is widely found exclusively in certain regions of Africa and Asia. They are recognized as edible mushroom within Southeast Asia as well. But it is often misidentified based on morphology by the local communities especially in Malaysia for which is a highly poisonous mushroom. Thus, it is necessary to study the genus for Malaysia with the synergy of using both morphological and molecular identification. In this study, we aim to describe another new species as an addition to the genus found within Sabah, Malaysia. We generated two new sequences (nrLSU and mtSSU) for the new species and a total of 28 nrLSU and mtSSU sequences were retrieved from GenBank for the phylogenetic analysis using maximum likelihood and Bayesian inferences. We identified that the new collection from Sabah province is a new species and named as based on the termites found in the mound. A phylogeny tree made from the concatenated genes of LSU and mtSSU suggests that is closely related to from China. According to our results, the combination of molecular and morphology proved to be a robust approach to re-evaluate the taxonomic status of species in Malaysia. Additional surveys are needed to verify the species diversity and clarify their geographic distribution.
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http://dx.doi.org/10.1080/12298093.2020.1738743DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178835PMC
March 2020

in Korea: New Records and a New Species.

Mycobiology 2019 18;47(4):368-377. Epub 2019 Sep 18.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Korea.

The genus (Agaricales, Basidiomycota) is easy to recognize at the genus level because of big, fleshy basidiocarps with squamules covering the pileus; a single or double annulus; and big, thick-walled basidiospores with a germ pore. However, morphological identification is often unreliable in due to similar morphological features among species. Due to the uncertainty of previous morphological identification in the genus it is necessary to re-examine Korean using molecular data. We re-examined 34 specimens collected from 2012 to 2018 in Korea using a reverse taxonomic approach, whereby species identification was first done based on the internal transcribed spacer (ITS) region analysis, followed by morphological confirmation. We identified the presence of four species: , . , . , and sp. nov. Two species ( and . ) were previously unrecorded from Korea and is a new species. Detailed descriptions of all four species and taxonomic key are provided in this study. and are distributed through the country, but and are distributed only in limited areas. According to our results, the combination of ITS locus and morphology proved to be a robust approach to evaluate the taxonomic status of species in Korea. Additional surveys are needed to verify the species diversity and clarify their geographic distribution.
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http://dx.doi.org/10.1080/12298093.2019.1663122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6968693PMC
September 2019

Ultrasmall SnS Quantum Dots Anchored onto Nitrogen-Enriched Carbon Nanospheres as an Advanced Anode Material for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2020 Feb 29;12(6):7114-7124. Epub 2020 Jan 29.

Department of Chemical Engineering , Hanyang University , Seoul 04763 , Republic of Korea.

Structural pulverization of metal chalcogenides such as Sn-based compounds is a serious issue for development of high-performance anode materials and results in serious capacity fading during continuous charge and discharge cycles. In this work, we synthesize ultrasmall SnS quantum dots (QDs) anchored onto nitrogen-enriched carbon (NC) nanospheres through facile hydrothermal and carbonization processes to prepare a progressive anode material for sodium-ion batteries. The optimized SnS [email protected] electrode delivered an initial discharge capacity of 281 mAh g at 100 mA g and exhibited excellent cycling stability with a capacity retention of 75% after 500 cycles at a high current density of 1000 mA g. Ex situ XRD, XPS, FE-SEM, TEM measurements, and kinetics study were performed to unveil the sodium storage mechanism of the SnS [email protected] electrode. A sodium-ion full cell assembled with an SnS [email protected] anode and a NaV(PO) cathode exhibited high capacity and good cycling stability. Such a superior electrochemical performance of SnS [email protected] can be attributed to the synergistic effects of NC and SnS QDs where NC serves as a conducting matrix to support SnS QDs and helps avoid structural degradation. This work provides a promising strategy to resolve the pulverization issue of alloying and conversion-type anode materials.
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http://dx.doi.org/10.1021/acsami.9b18997DOI Listing
February 2020

The diversity and ecological roles of Penicillium in intertidal zones.

Sci Rep 2019 09 19;9(1):13540. Epub 2019 Sep 19.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, 08826, South Korea.

Members of the genus Penicillium are commonly isolated from various terrestrial and marine environments, and play an important ecological role as a decomposer. To gain insight into the ecological role of Penicillium in intertidal zones, we investigated the Penicillium diversity and community structure using a culture-dependent technique and a culture independent metagenomic approach using ITS (ITS-NGS) and partial β-tubulin (BenA-NGS) as targets. The obtained isolates were tested for halotolerance, enzyme activity, and polycyclic aromatic hydrocarbons (PAHs) degradation. A total of 96 Penicillium species were identified from the investigated intertidal zones. Although the BenA-NGS method was efficient for detecting Penicillium, some species were only detected using conventional isolation and/or the ITS-NGS method. The Penicillium community displayed a significant degree of variation relative to season (summer and winter) and seaside (western and southern coast). Many Penicillium species isolated in this study exhibited cellulase and protease activity, and/or degradation of PAHs. These findings support the important role of Penicillium in the intertidal zone for nutrient recycling and pollutant degradation.
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http://dx.doi.org/10.1038/s41598-019-49966-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753150PMC
September 2019

Increased frequency of CD4CD57 senescent T cells in patients with newly diagnosed acute heart failure: exploring new pathogenic mechanisms with clinical relevance.

Sci Rep 2019 09 9;9(1):12887. Epub 2019 Sep 9.

Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.

Recent animal studies showed T cells have a direct pathogenic role in the development of heart failure (HF). However, which subsets of T cells contribute to human HF pathogenesis and progression remains unclear. We characterized immunologic properties of various subsets of T cells and their clinical implications in human HF. Thirty-eight consecutive patients with newly diagnosed acute HF (21 males, mean age 66 ± 16 years) and 38 healthy control subjects (21 males, mean age 62 ± 12 years) were enrolled. We found that pro-inflammatory mediators, including CRP, IL-6 and IP-10 and the frequencies of CD57 T cells in the CD4 T cell population were significantly elevated in patients with acute HF compared to control subjects. A functional analysis of T cells from patients with acute HF revealed that the CD4CD57 T cell population exhibited a higher frequency of IFN-γ- and TNF-α- producing cells compared to the CD4CD57 T cell population. Furthermore, the frequency of CD4CD57 T cells at baseline and its elevation at the six-month follow-up were significantly related with the development of cardiovascular (CV) events, which were defined as CV mortality, cardiac transplantation, or rehospitalization due to HF exacerbation. In conclusion, CD4CD57 senescent T cells showed more inflammatory features and polyfunctionality and were associated with clinical outcome in patients with acute HF. More detailed study for senescent T cells might offer new opportunities for the prevention and treatment of human HF.
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http://dx.doi.org/10.1038/s41598-019-49332-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6733929PMC
September 2019

Three Unrecorded Species Belonging to Section from Marine Environments in Korea.

Mycobiology 2019 16;47(2):165-172. Epub 2019 Apr 16.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea.

Species that belong to section are commonly found in various terrestrial environments, but only a few have been reported in marine environments. Because the number of species reported in marine environments is increasing, we investigated the diversity of section in marine environments in Korea. Based on sequence analyses of β-tubulin and calmodulin loci, 21 strains of section were identified as , , , , cf. , , , and . Three of them were confirmed as new to Korea: , , and . Here, we have provided detailed morphological descriptions of these unrecorded species.
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http://dx.doi.org/10.1080/12298093.2019.1601330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6691904PMC
April 2019

Thermoplastic Polyurethane Elastomer-Based Gel Polymer Electrolytes for Sodium-Metal Cells with Enhanced Cycling Performance.

ChemSusChem 2019 Oct 19;12(20):4645-4654. Epub 2019 Sep 19.

Department of Chemical Engineering, Hanyang University, Seoul, 04763, Republic of Korea.

Sodium batteries have been recognized as a promising alternative to lithium-ion batteries. However, the liquid electrolyte used in these batteries has inherent safety problems. Polymer electrolytes have been considered as safer and more reliable electrolyte systems for rechargeable batteries. Herein, a thermoplastic polyurethane elastomer-based gel polymer electrolyte with high ionic conductivity and high elasticity was reported. It had an ambient-temperature ionic conductivity of 1.5 mS cm and high stretchability, capable of withstanding 610 % strain. Coordination between Na ions and polymer chains increased the degree of salt dissociation in the gel polymer electrolyte compared with the liquid electrolyte. An Na/Na V (PO ) cell assembled with gel polymer electrolyte exhibited good cycling performance in terms of discharge capacity, cycling stability, and rate capability, which was owing to the effective trapping ability of organic solvents in the polymer matrix and uniform flux of sodium ions through the gel polymer electrolyte.
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http://dx.doi.org/10.1002/cssc.201901799DOI Listing
October 2019

Neutrophil-Lymphocyte Ratio as a Predictor of Venous Thromboembolism after Total Knee Replacement.

J Knee Surg 2021 Jan 8;34(2):171-177. Epub 2019 Aug 8.

Department of Internal Medicine, Soonchunhyang University College of Medicine, Cheonan, South Korea.

Venous thromboembolism (VTE) is a potentially serious complication after total knee replacement (TKR), and recent guideline recommends thromboprophylaxis for VTE after TKR. The neutrophil-lymphocyte ratio (NLR) has emerged as a simple and new prognostic biomarker for several cardiovascular diseases. This study was performed to investigate the precise incidence of postoperative VTE and the role of NLR for predicting VTE in patients receiving thromboprophylaxis after TKR. We retrospectively enrolled 264 patients undergoing TKR who underwent routine screening enhanced pulmonary artery and lower extremity venography computed tomography (CT) scan within 7 postoperative days. Biochemical tests were performed within 2 weeks prior to surgery, and the NLR was defined as the absolute neutrophil count in peripheral blood divided by lymphocyte count. All patients received thromboprophylaxis with enoxaparin postoperatively. Of 264 patients, 102 (38.6%) were diagnosed with deep vein thrombosis (DVT) or pulmonary embolism on CT scan. Preoperative NLR was significantly higher in patients with postoperative VTE compared with that in patients without VTE (2.57 ± 1.59 vs. 2.11 ± 1.10,  = 0.011). Receiver operating characteristic curve analysis showed that a preoperative NLR of 1.90 was the best cutoff value for the prediction of postoperative VTE (sensitivity 57.8%, specificity 55.6%, and area under curve 0.589). In the multivariate analysis, a preoperative NLR ≥1.90 was a sole independent predictor of postoperative VTE (odds ratio: 1.95, 95% computed tomography: 1.16-3.31,  = 0.013). The present study shows a higher incidence of VTE (38.6%) after TKR in patients receiving thromboprophylaxis than that reported in previous studies. Furthermore, preoperative NLR was significantly higher in patients with postoperative VTE, and a high preoperative NLR (≥1.90) was an independent predictor of VTE after TKR. NLR measurement may be a simple and useful method for the prediction of VTE in patients undergoing TKR.
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http://dx.doi.org/10.1055/s-0039-1694043DOI Listing
January 2021

Update on heart failure management and future directions.

Korean J Intern Med 2019 07 1;34(4):944. Epub 2019 Jul 1.

Division of Cardiology, Hallym University Dongtan Sacred Heart Hospital, Hwaseong, Korea.

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http://dx.doi.org/10.3904/kjim.2018.428.e1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610194PMC
July 2019

Perioperative myocardial injury in revascularized coronary patients who undergo noncardiac surgery.

PLoS One 2019 27;14(6):e0219043. Epub 2019 Jun 27.

Department of Thoracic and Cardiovascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.

Background: Whether high-sensitivity cardiac troponin elevation during the perioperative period is associated with poor clinical outcome in revascularized coronary patients who undergo noncardiac surgery remains unclear. We investigated the effects of perioperative troponin elevation on the long-term clinical outcomes of patients with a history of coronary revascularization.

Methods: We analyzed patients whose pre- or postoperative high-sensitivity cardiac troponin I (hs-cTnI) assay results were available. Patients were divided into two groups according to hs-cTnI levels. The patient groups were analyzed separately according to whether hs-cTnI was assessed preoperatively or postoperatively. The primary outcome was all-cause death during the follow-up period.

Results: Median follow-up duration was 25 months (interquartile range 11-50). In the propensity-matched analysis, the risk of all-cause death during follow-up was higher in the group with elevated hs-cTnI group than in the normal group (12.7% vs 6.3%; hazard ratio [HR], 2.67; 95% confidential interval [CI], 1.04-6.82; p = 0.04). In the propensity-matched analysis of preoperative hs-cTnI levels, we found no significant difference between the groups in the rate of all-cause death (12.9% vs. 11.9%; HR, 1.06; 95% CI, 0.45-2.50; p = 0.89). In the postoperative propensity-matched analysis, all-cause death was higher in patients with elevated hs-cTnI than in those with normal levels (14.9% vs. 5.9%; HR, 2.80; 95% CI, 1.01-7.77; p = 0.048).

Conclusion: In revascularized coronary patients who underwent noncardiac surgery, postoperative (but not preoperative) hs-cTnI elevation was associated with all-cause death during follow-up. Larger datasets are needed to support this finding.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0219043PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597116PMC
February 2020

The Influence of Microfungi on the Mycelial Growth of Ectomycorrhizal Fungus .

Microorganisms 2019 Jun 7;7(6). Epub 2019 Jun 7.

School of Biological Sciences and Institute of Microbiology, College of Natural Sciences, Seoul National University, Seoul 08826, Korea.

Pine mushroom () is one of the most valued ectomycorrhizal fungi in Asia because of its unique pine-like aroma; however, due to exceptionally slow growth of its mycelia in artificial conditions, its cultivation has been largely deemed as not possible. Previous studies have shown that some bacteria and a few species associated with pine mushroom promoted the growth of isolate, but this effect is relatively unexplored. In this study, we investigated the diversity of microfungi in the fairy ring of and their effect on the growth of isolate. From 184 fungal isolates, 28 species were identified based on suitable molecular markers. was most frequently observed (16 species), followed by (4 species). Five Zygomycota species showed a high promoting effect on the growth of while the effects of ascomycetes were mixed. The microfungi that promote the growth of can be useful for forest nursery and artificial cultivation of .
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http://dx.doi.org/10.3390/microorganisms7060169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617177PMC
June 2019

The Past, Present and Future of Cardiac Resynchronization Therapy.

Korean Circ J 2019 May;49(5):384-399

The Christ Hospital Heart and Vascular Center, Cincinnati, OH, USA.

Cardiac resynchronization therapy (CRT) has revolutionized the care of the patients with heart failure with reduced ejection fraction and electrical dyssynchrony. The current guidelines for patient selection include measurement of left ventricular systolic function, QRS duration and morphology, and functional classification. Despite consistent and increasing evidence supporting CRT use in appropriate patients, CRT has been underutilized. Notwithstanding the heterogeneous definitions of non-response, more than one-third of patients demonstrate a lack of echocardiographic reverse remodeling or poor clinical outcome following CRT. Since the causes of this non-response are multifactorial, it will require multidisciplinary efforts to overcome including optimal patient selection, procedural strategies, as well as optimizing post-implant care in patients undergoing CRT. The innovations of novel pacing approaches combined with advanced imaging technologies may eventually offer a personalized CRT system uniquely tailored to each patient's dyssynchrony signature.
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http://dx.doi.org/10.4070/kcj.2019.0114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511527PMC
May 2019

Fungal Diversity and Enzyme Activity Associated with the Macroalgae, .

Mycobiology 2019 Mar 1;47(1):50-58. Epub 2019 Mar 1.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea.

a brown macroalgae species, has recently become a serious environmental problem on the coasts of Korea. In an effort to solve this problem, fungal diversity associated with decaying was investigated and related β-glucosidase and endoglucanase activities were described. A total of 233 fungal strains were isolated from . at 15 sites and identified 89 species based on morphology and a multigene analysis using the internal transcribed spacer region (ITS) and protein-coding genes including actin (), β-tubulin (), calmodulin (), and translation elongation factor (). , and were the dominant genera, and and were the dominant species. Fifty-one species exhibited cellulase activity, with , , , , and Pleosporales sp. Five showing the highest enzyme activities. Further enzyme quantification confirmed that these species had higher cellulase activity than . , a fungal species described in previous studies. This study lays the groundwork for bioremediation using fungi to remove decaying seaweed from populated areas and provides important background for potential industrial applications of environmentally friendly processes.
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http://dx.doi.org/10.1080/12298093.2019.1580464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452909PMC
March 2019

Cellulosic Nanomaterial Production Via Fermentation by sp. SFCB22-18 Isolated from Ripened Persimmons.

J Microbiol Biotechnol 2019 Apr;29(4):617-624

School of Biological Sciences and Institution of Microbiology, Seoul National University, Seoul 08826, Republic of Korea.

Bacterial nanocellulose (BNC) which is generally synthesized by several species of bacteria has a wide variety of industrial uses, particularly in the food and material industries. However, the low levels of BNC production during the fermentation process should be overcome to reduce its production cost. Therefore, in this study, we screened and identified a new cellulose-producing bacterium, optimized production of the cellulose, and investigated the morphological properties of the cellulosic materials. Out of 147 bacterial isolates from ripened fruits and traditional vinegars, strain SFCB22-18 showed the highest capacity for BNC production and was identified as sp. based on 16S rRNA sequence analysis. During 6-week fermentation of the strain using an optimized medium containing 3.0% glucose, 2.5% yeast extract, 0.24% acetic acid, 0.27% NaHPO, and 0.5% ethanol at 30°C, about 5 g/l of cellulosic material was produced. Both imaging and IR analysis proved that the produced cellulose would be nanoscale bacterial cellulose.
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http://dx.doi.org/10.4014/jmb.1801.01005DOI Listing
April 2019

Cardiovascular outcome of breast cancer patients with concomitant radiotherapy and chemotherapy: A 10-year multicenter cohort study.

J Cardiol 2019 Aug 1;74(2):175-181. Epub 2019 Mar 1.

Division of Cardiology, Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Republic of Korea. Electronic address:

Background: Cancer treatment increases the risk of cardiovascular (CV) events. However, the long-term CV outcome of breast cancer patients who undergo radiotherapy and chemotherapy concomitantly is unknown. This study aimed to determine the incidence and risk factors of CV events among these patients.

Methods: Six hundred sixty consecutive breast cancer patients older than 50 years from November 2005 to September 2015, were enrolled in four university hospitals. The primary endpoint was CV events including CV mortality, myocardial infarction, heart failure, and stroke. CV events occurred in 14 (2.1%) patients during the follow-up period (median, 47.1 months).

Results: Left-side irradiation was associated with increased risk of CV events in patients with doxorubicin dose ≥250mg/m but not in patients with doxorubicin dose <250mg/m. On multivariable analysis, concomitant left-side irradiation with doxorubicin dose ≥250mg/m and hypertension were independent risk factors for CV events.

Conclusion: The risk of CV events was further increased with concomitant left-side irradiation and doxorubicin ≥250mg/m in breast cancer patients.
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http://dx.doi.org/10.1016/j.jjcc.2019.02.001DOI Listing
August 2019

Update on heart failure management and future directions.

Korean J Intern Med 2019 Jan 28;34(1):11-43. Epub 2018 Dec 28.

Division of Cardiology, Hallym University Dongtan Sacred Heart Hospital, Hwaseong, Korea.

Heart failure (HF) is an important cardiovascular disease because of its increasing prevalence, significant morbidity, high mortality, and rapidly expanding health care cost. The number of HF patients is increasing worldwide, and Korea is no exception. There have been marked advances in definition, diagnostic modalities, and treatment of HF over the past four decades. There is continuing effort to improve risk stratification of HF using biomarkers, imaging and genetic testing. Newly developed medications and devices for HF have been widely adopted in clinical practice. Furthermore, definitive treatment for end-stage heart failure including left ventricular assist device and heart transplantation are rapidly evolving as well. This review summarizes the current state-of-the-art management for HF and the emerging diagnostic and therapeutic modalities to improve the outcome of HF patients.
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http://dx.doi.org/10.3904/kjim.2018.428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325445PMC
January 2019

Diversity and effect of Trichoderma isolated from the roots of Pinus densiflora within the fairy ring of pine mushroom (Tricholoma matsutake).

PLoS One 2018 7;13(11):e0205900. Epub 2018 Nov 7.

School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea.

Pine mushroom (PM, Tricholoma matsutake) is an important ectomycorrhizal fungus in Asia primarily due to its value as a food delicacy. Recent studies have shown that fairy rings of PM have distinctive fungal communities, which suggests that other fungi influence the growth of PM. Trichoderma is a well-known saprotrophic fungus commonly found in pine roots within PM fairy rings; however, little is known about the diversity of Trichoderma associated with PM and how these species influence PM growth. This study focused on diversity of Trichoderma isolated from pine roots within PM fairy rings and how these species affect the growth of PM isolate. Based on tef1a phylogenetic analyses, nine Trichoderma species (261 isolates) were identified. Trichoderma songyi and T. spirale were the dominant species, and Trichoderma community varied geographically. Growth experiments indicated that metabolites from five Trichoderma species had a significant influence on the growth of PM isolates. Metabolites of two Trichoderma species increased PM growth, while those of three Trichoderma species suppressed the growth. Within the fairy rings, Trichoderma that had a positive or neutral effect comprised the majority of Trichoderma communities. The results of this study suggest that various Trichoderma species co-exist within PM fairy rings and that these species influence PM growth.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0205900PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6221287PMC
April 2019

New Report of Three Unrecorded Species in Species Complex in Korea.

Mycobiology 2018 24;46(3):177-184. Epub 2018 Aug 24.

Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University, Seoul, Korea.

The genus (Hypocreaceae, Ascomycota) consists of globally distributed fungi. Among them, , one of the most commonly collected species, had been known as a polyphyletic or aggregate species. However, a total of 19 species were determined from the polyphyletic groups of . Thus, we explored Korean "" specimens that were collected in 2013-2014. These specimens were re-examined based on a recent study with translate elongation factor 1-alpha (EF1α) sequences to reveal cryptic species in Korea. As a result, four different species, , and , were identified. Except , the other three species have not been reported in Korea. In this work, we describe these species and provide figures.
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http://dx.doi.org/10.1080/12298093.2018.1497792DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171446PMC
August 2018

A systematic revision of the ectomycorrhizal genus Laccaria from Korea.

Mycologia 2018 Sep-Oct;110(5):948-961. Epub 2018 Sep 21.

a School of Biological Sciences and Institute of Microbiology, Seoul National University , Seoul 08826 , Republic of Korea.

Species of Laccaria (Hydnangiaceae, Basidiomycota) are important in forest ecosystems as ectomycorrhizal fungi. Nine of the 75 described Laccaria species worldwide been reported from Korea. Most of these have European and North American names, and their identities are based solely on morphological features. To evaluate the taxonomy of Korean Laccaria, we used 443 specimens collected between 1981 and 2016 in a phylogenetic analysis based on sequence data from nuc rDNA internal transcribed spacer ITS1-5.8S-ITS2 rDNA (ITS) region, nuc 28S rDNA (28S), RNA polymerase II subunit 2 (rpb2), and translation elongation factor 1-α (tef1). Ten Laccaria species were identified. Three of these were previously reported from Korea: L. bicolor, L. tortilis, and L. vinaceoavellanea. Laccaria alba, L. japonica, and L. murina are confirmed as new reports from Korea. Lastly, four new Laccaria species are described: L. araneosa, L. parva, L. torosa, and L. versiforma. This study supports the general contention that Asian species of ectomycorrhizal fungi may not be conspecific with morphologically similar species from Europe and North America. Furthermore, identification based on morphology alone is often unreliable in Laccaria due to considerable overlap of characters among species. Thus, use of molecular methods is necessary for effective identification. Illustrations of the four newly described species and a taxonomic key to species of Laccaria in Korea are provided.
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http://dx.doi.org/10.1080/00275514.2018.1507542DOI Listing
March 2019

Predictive Value of Intraoperative Pulmonary Vascular Resistance in Liver Transplantation.

Liver Transpl 2018 12;24(12):1680-1689

Department of Medicine, Dongtan Sacred Heart Hospital, Hallym University School of Medicine, Seoul, South Korea.

We aimed to evaluate the association between intraoperative pulmonary vascular resistance (PVR) and clinical outcome of liver transplantation (LT). Cardiovascular involvement of end-stage liver disease is relatively common, and hemodynamic instability during LT can be fatal to recipients. However, the clinical impact of intraoperative PVR in LT remains undetermined. A total of 363 adult recipients with intraoperative right heart catheterization from January 2011 to May 2016 were analyzed. Patients were divided into 2 groups according to PVR. Two separate analyses were performed according to the time point of measurement: at the beginning and at the end of LT. The primary outcome was all-cause death or graft failure during the follow-up period. Increased PVR was observed in 11.8% (43/363) of recipients at the beginning and 12.7% (46/363) of recipients at the end of LT. PVR at the beginning of LT had no significant effect on the rate of death or graft failure in the multivariate analysis (hazard ratio [HR], 1.24; 95% confidence interval [CI], 0.64-2.38; P = 0.52). In contrast, PVR at the end of LT was significantly associated with death or graft failure during the overall follow-up period (HR, 2.00; 95% CI, 1.13-3.54; P = 0.02). In conclusion, PVR at the end of LT, rather than the beginning, is associated with clinical outcome. Larger trials are needed to support this finding.
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http://dx.doi.org/10.1002/lt.25341DOI Listing
December 2018
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