Publications by authors named "Myriam De la Garza Ramos"

24 Publications

  • Page 1 of 1

Antimicrobial Effect of Calcium Hydroxide Combined with Electrolyzed Superoxidized Solution at Neutral pH on Growth.

Biomed Res Int 2021 9;2021:6960143. Epub 2021 Nov 9.

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, Laboratorio de Inmunología y Virología, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, CP 66451, Mexico.

Objective: To evaluate the effect of the combination of calcium hydroxide (Ca(OH)) and a novel electrolyzed superoxidized solution at neutral pH, known as OxOral® on growth in root canals.

Methods: Sixty human teeth were used, from which root canals were infected and randomly divided into the following treatment groups: saline solution, saline solution plus Ca(OH), OxOral®, and OxOral® plus Ca(OH).

Results: A permanent reduction in bacterial growth was observed at days 1, 6, 12, and 18 after OxOral® plus Ca(OH) treatment from 4.4 ± 0.074 log CFU/mL to 0.0 ± 0.001 log CFU/mL. In addition, alkaline conditions maintenance was observed from application time (pH = 12.2 ± 0.033) to 18 d posttreatment (pH = 12.6 ± 0.083).

Conclusion: The combination of OxOral® and Ca(OH) provides an alkaline pH and inhibits growth into the root canals. Our study opens the possibility for further research on the use of OxOral® in endodontic therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/6960143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8595027PMC
January 2022

Antimicrobial activity and inhibition of biofilm formation in vitro and on human dentine by silver nanoparticles/carboxymethyl-cellulose composites.

Arch Oral Biol 2020 Dec 16;120:104943. Epub 2020 Oct 16.

Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolas de los Garza, Nuevo León, C.P. 66455, Mexico. Electronic address:

Objective: To evaluate the antimicrobial activity of a silver nanoparticles/carboxymethyl-cellulose (AgNPs/CMC) composite on in vitro and dentine disc heterogeneous biofilms.

Design: AgNPs/CMC composite effect on normal human gingival fibroblast cells (HGF) viability was determined by the MTT reduction assay. In addition, we evaluated the antimicrobial effect of AgNPs/CMC composite on Candida albicans, Enterococcus faecalis, and Fusobacterium nucleatum growth in vitro and heterogeneous biofilms, as well as dentine disc biofilms.

Results: Quasi-spherical AgNPs/CMC composites, with a mean 22.3 nm particle-size were synthesized. They were not toxic to HGF cells at concentrations tested that were antimicrobial, however they caused significant cytotoxicity (89 %, p <  0.05) at concentrations > 15 μg/mL. In vitro, they inhibited up to 67 %, 66 %, and 96 % C. albicans, E. faecalis, and F. nucleatum growth at concentrations ranging from 1.2 μg/mL to 9.6 μg/mL, as compared with untreated control. We also demonstrated significant (p <  0.05) 58 % biofilm reduction by 4.8 μg/mL AgNPs/CMC composite on human dentine discs.

Conclusion: AgNPs/CMC composite showed anti biofilm activity on monocultures, heterogenous cultures, and dentine discs, resulting a potentially effective alternative to prevent and eliminate infections after endodontic treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.archoralbio.2020.104943DOI Listing
December 2020

Electrochemical Behavior of Ti6Al4V Alloy Used in Dental Implants Immersed in and Solutions.

Materials (Basel) 2020 Sep 21;13(18). Epub 2020 Sep 21.

Universidad Autonoma de Nuevo Leon, FIME-Centro de Investigación e Innovación en ingeniería Aeronáutica (CIIIA), Av. Universidad s/n, Ciudad Universitaria, San Nicolás de los Garza, N.L. 66455, Mexico.

The titanium alloy, Ti6Al4V, is used in dentistry for dental implants because of its excellent resistance to corrosion and its high biocompatibility. However, periimplantitis is considered the main reason for treatment failure. The Ti6Al4V alloy was used to study the corrosion behavior for dental implant applications, using an experimental arrangement of three electrodes with the bacteria and , in addition to Ringer's lactate as electrolytes, at 37 °C and a pH of 5.6. Their electrochemical behavior was studied by open circuit potential (OCP) and cyclic potentiodynamic polarization (CPP) according to ASTM G3-14 and ASTM G61-11, respectively. Scanning electron microscopy (SEM) was employed to determine the morphology of the alloy studied. An experimental model, in situ, was established with the bacteria present in an oral environment to understand the electrochemical behavior of the alloy used in dental implants. The greatest corrosion in Ti6Al4V alloy was produced by the medium that contained the bacterium , which is considered a primary colonizer. In addition, the Ti6Al4V alloy presented uniform corrosion in the three solutions at the different exposure times showing a negative hysteresis in CPP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ma13184185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560454PMC
September 2020

Chlamydia trachomatis in the gingival sulcus and pharynx in patients of Northeast Mexico.

Clin Exp Dent Res 2020 08 27;6(4):415-419. Epub 2020 Mar 27.

Facultad de Odontología/Posgrado de Periodoncia y Unidad de Odontología Integral y Especialidades del CIDICS, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, Mexico.

Background: The oral microenvironment provides the conditions for the establishment of microorganisms not usually considered residents of the normal oral microbiota. Sexually transmitted microorganisms such as Chlamydia trachomatis can adhere to any mucosal surface and ascend to reach appropriate locations to survive and develop symptomatic infections.

Materials And Methods: To determine the presence of C. trachomatis, direct immunofluorescence of this microorganism was carried out in 76 randomly selected patients attending a periodontal clinic during a period of 1 year. Samples from the gingival sulcus and the pharynx were collected for detection of C. trachomatis. Patients who attended the periodontal clinic were divided into two groups: those without periodontitis and those with periodontitis. For the purpose of performing other statistical analyses, all patients were also divided by gender and age.

Results: From the total of 76 patients, in the group without periodontitis, 61% were positive for C. trachomatis in the gingival sulcus and 63.4% in the pharynx; in the periodontitis group, 45.7% were positive in the sulcus and 40% in the pharynx. When we compared patients by gender or age, no statistical difference was found.

Conclusions: The prevalence of C. trachomatis in this group was 53.9% in the gingival sulcus and pharynx of the studied patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cre2.290DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453775PMC
August 2020

Prevalence of human papillomavirus in the oral cavity of an indigenous community from Southwest México.

Infect Genet Evol 2020 09 17;83:104283. Epub 2020 Mar 17.

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, Nuevo León C.P. 66455, México. Electronic address:

Human papilloma virus (HPV) is a DNA virus associated with the development of cervical, penile, anal, vulvar, and oral cancers. In recent years, there has been an increase in oral cancer, which could be due to changes in sexual behavior in the general population. In México, there is scarce information on this regard, which prompted us to study HPV infection prevalence in the oral cavity of an indigenous community from the municipality of Siltepec, Chiapas, Mexico. Oral samples from 198 individuals were obtained with cytobrush for virus detection by nested PCR, using MY09/MY11 and GP5+/GP6+ primers, and positive samples were sequenced for HPV genotyping. We observed 12.1% HPV infection prevalence, which depended on gender, number of sexual partners, lack of using condoms, and oral sex practices. In contrast, no significant association between HPV infection and tobacco or alcohol consumption was detected. Furthermore, sequencing analyzes were performed where HPV-13 (21/24), -16 (2/24), -32 (1/24), -81 (1/24), and -83 (1/24) were evidenced and HPV-16 European/Asian and Asian/American E6 variants identified. These results demonstrated an important prevalence of HPV infection in the oral cavity of a Mexican indigenous community, where the predominant genotypes were associated with benign pathologies, and showed that high-risk genotype variants derived from different lineages.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.meegid.2020.104283DOI Listing
September 2020

Antimicrobial and Anti-Biofilm Effect of an Electrolyzed Superoxidized Solution at Neutral-pH against .

Biomed Res Int 2019 18;2019:6154867. Epub 2019 Dec 18.

Laboratory of Immunology and Virology, School of Biological Sciences, Autonomous University of Nuevo Leon, Pedro de Alba and Manuel L. Barragán St., Cd. Universitaria, San Nicolás de los Garza, N.L., Monterrey, 66450, Mexico.

The presence of in the oral cavity has been associated to the failure of antimicrobial therapy in patients with gastrointestinal infection and the development of oral diseases. However, it has been reported that the maintenance of good oral hygiene can improve the therapeutic success rates, where the use of mouthwashes with anti- activity would help to achieve it. The aim was to evaluate the antimicrobial activity of OxOral® mouthwash against and its effect on biofilm formation. The minimum inhibitory concentration (MIC) of OxOral® (pH = 6.4-7.5, ORP = 650-900 mV) against was calculated testing serial dilutions 0.117-15 ppm against 1 × 10 CFU/mL of (ATCC® 700824™) by broth microdilution method using 96-well plates. The biofilm formation was determined by the optical density measurement at 600 nm from coverslips stained with 0.1% crystal violet. The gene expression of , , , and were analyzed by RT-qPCR. OxOral® cytotoxicity was evaluated in a human gingival fibroblast cell line by MTT assay. MIC was of 3.75 ppm, with 99.7 ± 7.7% bacterial growth inhibition. In the negative control, the biofilm formation was observed, whereas when bacteria were treated with OxOral® at 0.234, 0.469, and 0.938 ppm, an inhibition of 35.5 ± 0.9%, 89.1 ± 1.2%, and 99.9 ± 5.5% were obtained, respectively. The gene expression analysis showed that , and genes were down-regulated with OxOral® compared with control ( < 0.05). Low cytotoxicity of 16.5 ± 7.6% was observed at the highest dose (15 ppm); no significant differences were observed from 15 to 0.469 ppm compared to the control of untreated cells ( > 0.05). Our results reveal an important anti- activity of OxOral® and open the possibility of its therapeutic use new studies, which would increase the success rate of conventional therapies against .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2019/6154867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6942767PMC
June 2020

Molecular detection of based on the presence of and virulence genes in dental plaque from patients with periodontitis.

J Dent Sci 2019 Jun 27;14(2):163-170. Epub 2019 Mar 27.

Universidad Autónoma de Nuevo León, Facultad de Odontología y Unidad de Odontología Integral y Especialidades del CIDICS, Av. Dr. Aguirre Pequeño y Silao S/N, Colonia Mitras Centro, CP. 64460, Monterrey, N.L., Mexico.

Background/purpose: () infection is the most common in the world and is associated with various gastrointestinal pathologies, including chronic gastritis, peptic ulcers, and gastric cancer. The prevalence is associated with socioeconomic conditions, with this infection being more common in developing countries than in developed countries. The presence and permanence of in the oral cavity has been reported, but its role is controversial. The aim of this study was to determine the prevalence of in dental plaque of patients with periodontitis.

Materials And Methods: A cross-sectional study was carried out and Periodontal Screening and Recording (PSR) index was determined. 38 dental plaque samples were taken and total DNA was extracted and qPCR was performed.

Results: 60.5% of the samples (n = 23) were positive for the presence of by the amplification of the and genes. In addition, gene was detected in 21.7% (n = 5) of -positive. A significant relationship between periodontal status and oral infection was found ( ≤ 0.05); patients with initial and moderate periodontitis were the most affected with 39.1% and 30.4%, respectively.

Conclusion: Our results suggest that the prevalence of in the oral cavity could be related to the progression of periodontal disease. Therefore, oral hygiene and treatment for the elimination of oral could stop the progression of periodontal disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jds.2019.01.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562180PMC
June 2019

Immunoinformatics Approach to Design a Novel Epitope-Based Oral Vaccine Against .

J Comput Biol 2019 10 23;26(10):1177-1190. Epub 2019 May 23.

Laboratory of Immunology and Virology, School of Biological Sciences, Autonomous University of Nuevo Leon, Monterrey, Mexico.

is an infectious agent that colonizes the gastric mucosa of half of the population worldwide. This bacterium has been recognized as belonging to group 1 carcinogen by the World Health Organization for the role in development of gastritis, peptic ulcers, and cancer. Due to the increase in resistance to antibiotics used in the anti- therapy, the development of an effective vaccine is an alternative of great interest, which remains a challenge. Therefore, a rational, strategic, and efficient vaccine design against is necessary where the use of the most current bioinformatics tools could help achieve it. In this study, immunoinformatics approach was used to design a novel multiepitope oral vaccine against . Our multiepitope vaccine is composed of cholera toxin subunit B (CTB) that is used as a mucosal adjuvant to enhance vaccine immunogenicity for oral immunization. CTB fused to 11 epitopes predicted of pathogenic (UreB, VacA, CagA, GGT, NapA, and OipA) and colonization (HpaA, FlaA, FecA, BabA, and SabA) proteins from . CKS9 peptide (CKSTHPLSC) targets epithelial microfold cells to enhance vaccine uptake from the gut barrier. All sequences were joined to each other by proper linkers. The vaccine was modeled and validated to achieve a high-quality three-dimensional structure. The vaccine design was evaluated as nonallergenic, antigenic, soluble, and with an appropriate molecular weight and isoelectric point. Our results suggest that our newly designed vaccine could serve as a promising anti- vaccine candidate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/cmb.2019.0062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6786345PMC
October 2019

Immunization with a Synthetic Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection.

Can J Infect Dis Med Microbiol 2019 1;2019:8595487. Epub 2019 Apr 1.

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, Mexico.

is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects. Furthermore, because there are no vaccines effective against , an appropriate vaccine design targeting conserved/essential genes must be identified. In the present study, a 50-52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against infection. For this, mice received an intraperitoneal injection of 100 g of peptide on the first week, followed by two weekly subcutaneous reinforcements and further 10 bacteria administration in the drinking water for 3 weeks. Thymic cells proliferative responses to concanavalin A, serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-, and TNF- cytokines, and IgG1, IgG2a, IgG2b, IgG3 IgM, and IgA immunoglobulins were evaluated. Significant ( < 0.05) increases on lymphoproliferation and spleen weights after immunization were observed. In contrast, infection significantly ( < 0.05) decreased lymphoproliferation, which was recovered in immunized mice. In addition, levels of serum TH1 and TH2 cytokines were not altered after immunization, except for the significant increase in IL-6 production in immunized and/or infected animals. Moreover, immunization correlated with plasma secretory IgA and IgG, whereas infection alone only elicited IgM antibodies. Peptide immunization protected 100% of mice against virulent . MVTLINNE peptide deserves further research as an approach to the prophylaxis of infection.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2019/8595487DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466936PMC
April 2019

Dental iron precipitates in patients with Type 2 diabetes.

Clin Exp Dent Res 2019 02 5;5(1):14-18. Epub 2018 Dec 5.

Facultad de Odontologia Universidad Autonoma de Nuevo Leon Monterrey Mexico.

Diabetes mellitus (DM) is a frequent worldwide disease. There are currently more than 46 million people who suffer this disease in North America and the Caribbean. The objective of this study was to determine if there is an association between DM and the presence of iron precipitates (Fe) in dental structure. The third molar was extracted for reasons that merit extraction from 40 individuals with and without DM to analyze dentin tissue. Horizontal and longitudinal slices of tooth samples were made and later stained with 10% potassium cyanoferrate. The samples were observed by optical microscope to identify basophilic elements. A nonparametric Spearman correlation was performed to find an association between the quantitative (gender, group, and dentinal tissue) and qualitative variables (gender). The Mann-Whitney U test was used to find differences in the means of the nonparametric variables in two different groups in relation to the value (<0.05). Iron elements were found in the predentin and circumpulpal dentin areas, and the results obtained showed a statistically significant difference between dentin tissue from patients with diabetes and those without. Individuals with Type 2 DM are prone to present iron precipitates in predentin and circumpulpal dentin tissue. Few iron elements were found in dental organs of individuals without DM.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cre2.150DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392848PMC
February 2019

Corrigendum to "Subchronic Infection of and Stimulates an Immune Response but Not Arthritis in Experimental Murine Model".

Int J Dent 2018 1;2018:6093609. Epub 2018 Oct 1.

Universidad Autónoma de Nuevo Leon, Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Facultad de Odontología, Calle Dr. Eduardo Aguirre Pequeño s/n, Colonia Mitras Centro, 64460 Monterrey, NL, Mexico.

[This corrects the article DOI: 10.1155/2017/2052938.].
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2018/6093609DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6188859PMC
October 2018

Salivary ammonia levels and Tannerella forsythia are associated with rheumatoid arthritis: A cross sectional study.

Clin Exp Dent Res 2017 Jun 7;3(3):107-114. Epub 2017 Jun 7.

Facultad de Farmacia Universidad Autónoma del Estado de Morelos Cuernavaca Morelos 62209 Mexico.

The aim of this paper is to evaluate the relationship of salivary ammonium levels and the presence of bacteria with rheumatoid arthritis (RA) clinical disease activity in a cross-sectional study of Mexican patients. From a periodontal and disease activity standpoint, 132 consecutive RA patients fulfilling clinical criteria were evaluated. Ammonia levels (including peptidyl arginine deiminase activity) were evaluated by colorimetric assay and the presence of , , and was evaluated by polymerase chain reaction (PCR) technique. After a multivariate analysis, adjusting for clinical and serological parameters, a significant association was only observed between severe periodontitis and probing depth with high RA disease activity. Additionally, in contrast to , the presence of was significantly associated with high disease RA activity even after multivariable adjustment analysis. There was also a significant increase in ammonium levels in the high RA activity group and a significant correlation between salivary ammonia and RA disease activity but not with autoantibody titers. Similarly, we observed a significant increase in the ammonium levels derived from the cultures of and , with respect to and cultures, or even healthy donors. These results suggest that RA activity is associated with severe periodontitis, high salivary ammonium levels and the presence of .
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cre2.68DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5719825PMC
June 2017

Persistence of Toxic Activity of Fermentation Extracts from var. after More Than Three Decades of Storage.

Int J Microbiol 2017 20;2017:5402748. Epub 2017 Nov 20.

Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Ciudad Universitaria, 66450 San Nicolás de los Garza, NL, Mexico.

This study was carried out to determine the persistence of toxicity of fermentation extracts of var. after more than three decades of storage. For this purpose, a population of was established. The mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified WHO protocol. The extracts with the highest mortality rate were determined in triplicate by their LD and LD. All extracts showed toxicity at the highest tested dose (1000 ppm) and some, such as strains 3260 and 3501, still killed larvae at doses as low as 0.01 ppm. These data are surprising because no study on the activity of toxic proteins after such a long storage time has been reported.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2017/5402748DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735669PMC
November 2017

Prevalence of Genotypes and Gene in Dental Plaque of Asymptomatic Mexican Children.

Biomed Res Int 2017 1;2017:4923640. Epub 2017 Nov 1.

Posgrado de Ortodoncia, Facultad de Odontología (FO), Universidad Autónoma de Nuevo León (UANL), Av. Dr. Aguirre Pequeño 1504, Col. Mitras Centro, 64460 Monterrey, NL, Mexico.

The variability in and genes has been related to the progression of the gastrointestinal disease; also the presence of in the oral cavity has been associated with periodontal disease in adults, but, in children without dyspeptic symptoms, little is known about this. We evaluated the prevalence of and the presence of / genotypes in the oral cavity of Mexican children without dyspeptic symptoms. The gingival status was measured, and dental plaque samples ( = 100) were taken. 38% of children were positive for 16S rRNA gene by qPCR. A significant association between oral infection and gingival status was observed ( < 0.001). In 34.6% (9/26) of mild gingivitis cases, genotype was found, while was typed in 50% (3/6) of moderate gingivitis. The prevalence among -positive children was 80.8% (21/26), 83.3% (5/6), and 16.7% (1/6) of cases of mild gingivitis, moderate gingivitis, and nongingivitis, respectively ( < 0.001). The + combinational genotype was the most detected in children with gingivitis. Our results suggest that the prevalence of and detection of genotypes-associated gastrointestinal disease in the oral cavity could be related to the progression of gingivitis in asymptomatic children.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2017/4923640DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687131PMC
July 2018

A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of -Solanine on Rat Bone Marrow Mesenchymal Stem Cells.

Bone Marrow Res 2017 22;2017:2170306. Epub 2017 Oct 22.

Instituto de Genética Humana, Facultad de Medicina, Pontificia Universidad Javeriana, Bogotá, Colombia.

Αlpha-solanine (-solanine) is a glycoalkaloid present in potato . It has been of particular interest because of its toxicity and potential teratogenic effects that include abnormalities of the central nervous system, such as exencephaly, encephalocele, and anophthalmia. Various types of cell culture have been used as experimental models to determine the effect of -solanine on cell physiology. The morphological changes in the mesenchymal stem cell upon exposure to -solanine have not been established. This study aimed to describe a reliable and reproducible model for assessing the structural changes induced by exposure of mouse bone marrow mesenchymal stem cells (MSCs) to different concentrations of -solanine for 24 h. The results demonstrate that nonlethal concentrations of -solanine (2-6 M) changed the morphology of the cells, including an increase in the number of nucleoli, suggesting elevated protein synthesis, and the formation of spicules. In addition, treatment with -solanine reduced the number of adherent cells and the formation of colonies in culture. Immunophenotypic characterization and staining of MSCs are proposed as a reproducible method that allows description of cells exposed to the glycoalkaloid, -solanine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2017/2170306DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671669PMC
October 2017

In Vitro Antimicrobial Activity and Downregulation of Virulence Gene Expression on Helicobacter pylori by Reuterin.

Probiotics Antimicrob Proteins 2018 06;10(2):168-175

Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, Laboratorio de Inmunología y Virología, Pedro de Alba y Manuel L. Barragán S/N. Cd. Universitaria, CP. 66450, San Nicolás de los Garza, N.L., México.

Helicobacter pylori is an infectious agent commonly associated with gastrointestinal diseases. The use of probiotics to treat this infection has been documented, however, their potential antimicrobial metabolites have not yet been investigated. In the present study, the effect of reuterin produced by Lactobacillus reuteri on H. pylori growth and virulence gene expression was evaluated. It was observed that reuterin caused significant (P < 0.05) H. pylori growth inhibition at concentrations from 0.08 to 20.48 mM, with minimal inhibitory concentrations (MICs) of 20.48 mM for H. pylori ATCC700824 and 10.24 mM for H. pylori ATCC43504. In a reuterin bacterial killing assay, it was observed that half of the MIC value for H. pylori (ATCC700824) significantly (P < 0.01) reduced colony numbers from 5.65 ± 0.35 to 3.78 ± 0.35 Log CFU/mL after 12 h of treatment and then increased them to 5.25 ± 0.23 Log CFU/mL at 24 h; at its MIC value (20.48 mM), reuterin abrogated (P < 0.01) H. pylori (ATCC700824) growth after 20 h of culture. In addition, reuterin significantly (P < 0.01) reduced H. pylori (ATCC 43504) colony numbers from 5.65 ± 0.35 to 4.1 ± 0.12 Log10 CFU/mL from 12 to 24 h of treatment and abrogated its growth at its MIC value (10.24 mM), after 20 h of treatment. Reuterin did not alter normal human gastric Hs738.St/Int cell viability at the concentrations tested for H. pylori strains. Furthermore, 10 μM reuterin was shown to significantly (P < 0.01) reduce mRNA relative expression levels of H. pylori virulence genes vacA and flaA at 3 h post-treatment, whose effect was higher at 6 h post-treatment, as measured by RT-qPCR. The observed direct antimicrobial effect and the downregulation of expression of virulence genes on H. pylori by reuterin may contribute to the understanding of the mechanisms of action of probiotics against H. pylori.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12602-017-9342-2DOI Listing
June 2018

Subchronic Infection of and Stimulates an Immune Response but Not Arthritis in Experimental Murine Model.

Int J Dent 2017 6;2017:2052938. Epub 2017 Jun 6.

Universidad Autónoma de Nuevo Leon, Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Facultad de Odontología, Calle Dr. Eduardo Aguirre Pequeño s/n, Colonia Mitras Centro, 64460 Monterrey, NL, Mexico.

Studies have proposed that and promote a nonspecific inflammatory response that could produce systemic disease. Oral inoculation of and on the immune and arthritis response was evaluated in BALB/C mice divided into four groups: (1) sham; (2) food contaminated with ; (3) complete Freund's adjuvant (CFA) + ; and (4) CFA alone. CFA was administered subcutaneously on days 1 and 14. The arthritis response was monitored for 21 days after day 14 of CFA administration. IL-1 and IL-6 were determined in serum. T cell activation was evaluated by CD25 in salivary lymph nodes or mouse spleen. Pad inflammation appeared by day 19 in the CFA group, but animals with bacteria inoculation presented a delay. A significant increase in IL-6 was found in Groups 3 and 4, but not with respect to IL-1. We observed an increase in CD25 in cells derived from cervical nodes and in animals with bacteria inoculation and CFA. A local immune response was observed in mice inoculated with and (T cell activation); a systemic response was observed with CFA. Since pad inflammation was delayed by bacterial inoculation this suggests that local T cell activation could decrease pad inflammation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2017/2052938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5476872PMC
June 2017

Miconia sp. Increases mRNA Levels of PPAR Gamma and Inhibits Alpha Amylase and Alpha Glucosidase.

Evid Based Complement Alternat Med 2016 13;2016:5123519. Epub 2016 Jul 13.

Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Pedro de Alba, s/n, Ciudad Universitaria, 66455 San Nicolas de los Garza, NL, Mexico.

Diabetes mellitus is a public health problem worldwide. For this reason, ethanolic extract of Miconia sp. from Oaxaca, Mexico, was selected in search of an alternative against this disease. The effect of Miconia sp. on mRNA expression of PPARγ on cell line 3T3-L1, its effect on alpha amylase and alpha glucosidase, lipid accumulation during adipogenesis, and cell viability on VERO cells were evaluated. The mRNA levels of PPARγ increased on 1.393 ± 0.008 folds, lipid accumulation was increased by 29.55% with Miconia sp. extract and 34.57% with rosiglitazone, and α-amylase and α-glycosidase were inhibited with IC50 values from 28.23 ± 2.15 μg/mL and 1.95 ± 0.15 μg/mL, respectively; the IC50 on antiproliferative activity on VERO cells was 314.54 ± 45.40 μg/mL. In case of α-amylase and α-glycosidase assays, IC50 (inhibitory concentration 50) refers to necessary extract amounts to inhibit 50% of enzymatic activity. On the other hand, on antiproliferative activity, IC50 (inhibitory concentration 50) refers to necessary extract amounts to inhibit 50% of cell proliferation. It was concluded that the compounds present in Miconia sp. ethanolic extract increase mRNA expression of PPARγ, inhibit α-amylase and α-glucosidase, and increase lipid accumulation. It constitutes an alternative as adjuvant in diabetes mellitus treatment; therefore, we recommend continuing identifying the compounds responsible for its promising in vivo antidiabetic activity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2016/5123519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961835PMC
August 2016

Antimicrobial properties and dental pulp stem cell cytotoxicity using carboxymethyl cellulose-silver nanoparticles deposited on titanium plates.

Acta Biomater Odontol Scand 2016 Dec 29;2(1):60-67. Epub 2016 Mar 29.

Posgrado de Ortodoncia, Facultad de Odontologia, Universidad Autonoma de Nuevo LeónMonterreyMexico.

To evaluate the antimicrobial properties and dental pulp stem cells (DPSCs) cytotoxicity of synthesized carboxymethyl cellulose-silver nanoparticles impregnated on titanium plates. The antibacterial effect of silver nanoparticles in a carboxymethyl cellulose matrix impregnated on titanium plates (Ti-AgNPs) in three concentrations: 16%, 50% and 100% was determined by adding these to bacterial cultures of and . The Ti-AgNPs cytotoxicity on DPSCs was determined using a fluorimetric cytotoxicity assay with 0.12% chlorhexidine as a positive control. Silver nanoparticles in all concentrations were antimicrobial, with concentrations of 50% and 100% being more cytotoxic with 4% cell viability. Silver nanoparticles 16% had a cell viability of 95%, being less cytotoxic than 0.12% chlorhexidine. Silver nanoparticles are a promising structure because of their antimicrobial properties. These have high cell viability at a concentration of 16%, and are less toxic than chlorhexidine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3109/23337931.2016.1160783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433232PMC
December 2016

Prevalence of caries and malocclusion in an indigenous population in Chiapas, Mexico.

Int Dent J 2015 Oct 18;65(5):249-55. Epub 2015 Sep 18.

San Francisco School of Dentistry, University of California, San Francisco, CA, USA.

Objectives: To assess the prevalence of caries and malocclusion in Mayan Mexican adolescents, 14-20 years of age, living in Chiapas, Mexico.

Methods: This was a cross-sectional, population-based, quantitative, epidemiological study. Sites were chosen to capture subjects representative of the state's Mayan population. A total of 354 subjects were recruited. Caries experience was quantified, via visual inspection, using the Decayed, Missing and Filled Surface (DMFS) index. Malocclusion was quantified using the Index of Complexity, Outcome and Need (ICON).

Results: Our data showed that 99% of the population had caries experience, with a median DMFS score of 8. Of the 99% with caries experience, over half had caries affecting more than five tooth surfaces. Thirty-seven per cent of the students had unmet orthodontic treatment need, and 46.46% presented a Class II, and 39.09% a Class III, anterior-posterior relationship.

Conclusions: Less than 1% of the population had any exposure to orthodontics, demonstrating the lack of access to care. Likewise, only 1% of the population was found to have no caries experience, exhibiting a large unmet treatment need. The median DMFS score of 8 was also high in comparison with the median DMFS in the USA of 6. Our data suggest a correlation between the lack of access to care and high prevalence of caries and malocclusion in Mexican Mayans who inhabit Chiapas, Mexico.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/idj.12177DOI Listing
October 2015

Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro.

Head Face Med 2015 Sep 3;11:29. Epub 2015 Sep 3.

Unidad de Odontología Integral y Especialidades, Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México.

Introduction: Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) play an important role in extracellular matrix mineralization, a complex process required for proper bone regeneration, one of the biggest challenges in dentistry. The purpose of this study was to evaluate the osteogenic potential of EGF and bFGF on dental pulp stem cells (DPSCs).

Material And Methods: Human DPSCs were isolated using CD105 magnetic microbeads and characterized by flow cytometry. To induce osteoblast differentiation, the cells were cultured in osteogenic medium supplemented with EGF or bFGF at a low concentration. Cell morphology and expression of CD146 and CD10 surface markers were analyzed using fluorescence microscopy. To measure mineralization, an alizarin red S assay was performed and typical markers of osteoblastic phenotype were evaluated by RT-PCR.

Results: EGF treatment induced morphological changes and suppression of CD146 and CD10 markers. Additionally, the cells were capable of producing calcium deposits and increasing the mRNA expression to alkaline phosphatase (ALP) and osteocalcin (OCN) in relation to control groups (p < 0.001). However, bFGF treatment showed an inhibitory effect.

Conclusion: These data suggests that DPSCs in combination with EGF could be an effective stem cell-based therapy for bone tissue engineering applications in periodontics and oral implantology.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s13005-015-0086-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558932PMC
September 2015

Antimicrobial Effect of Lactobacillus reuteri on Cariogenic Bacteria Streptococcus gordonii, Streptococcus mutans, and Periodontal Diseases Actinomyces naeslundii and Tannerella forsythia.

Probiotics Antimicrob Proteins 2015 Mar;7(1):1-8

Unidad de Odontología Integral y Especialidades, Centro de Investigación y Desarrollo en Ciencias de la Salud (CIDICS), Universidad Autónoma de Nuevo León (UANL), Ave. Carlos Canseco s/n con Ave. Gonzalitos, Mitras Centro, CP: 64460, Monterrey, Nuevo León, Mexico.

Lactic acid bacteria (LAB) are well known for their beneficial effects on human health in the intestine and immune system; however, there are few studies on the impact they can generate in oral health. The aim of this study was to test and compare in vitro antimicrobial activity of L. reuteri on pathogenic bacteria involved in the formation of dental caries: S. mutans, S. gordonii, and periodontal disease: A. naeslundii and T. forsythia. Also, we determined the growth kinetics of each bacterium involved in this study. Before determining the antimicrobial action of L. reuteri on cariogenic bacteria and periodontal disease, the behavior and cell development time of each pathogenic bacterium were studied. Once the conditions for good cell growth of each of selected pathogens were established according to their metabolic requirements, maximum exponential growth was determined, this being the reference point for analyzing the development or inhibition by LAB using the Kirby Bauer method. Chlorhexidine 0.12% was positive control. L. reuteri was shown to have an inhibitory effect against S. mutans, followed by T. forsythia and S. gordonii, and a less significant effect against A. naeslundii. Regarding the effect shown by L. reuteri on the two major pathogens, we consider its potential use as a possible functional food in the prevention or treatment of oral diseases.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12602-014-9178-yDOI Listing
March 2015

Zerovalent bismuth nanoparticles inhibit Streptococcus mutans growth and formation of biofilm.

Int J Nanomedicine 2012 24;7:2109-13. Epub 2012 Apr 24.

Instituto de Biotecnologia, Centro de Investigacion y Desarrollo en Ciencias de la Salud, CIDICS, Facultad de Odontologia, Universidad Autonoma de Nuevo Leon, UANL, Monterrey, Nuevo Leon, Mexico.

Background And Methods: Despite continuous efforts, the increasing prevalence of resistance among pathogenic bacteria to common antibiotics has become one of the most significant concerns in modern medicine. Nanostructured materials are used in many fields, including biological sciences and medicine. While some bismuth derivatives has been used in medicine to treat vomiting, nausea, diarrhea, and stomach pain, the biocidal activity of zerovalent bismuth nanoparticles has not yet been studied. The objective of this investigation was to analyze the antimicrobial activity of bismuth nanoparticles against oral bacteria and their antibiofilm capabilities.

Results: Our results showed that stable colloidal bismuth nanoparticles had 69% antimicrobial activity against Streptococcus mutans growth and achieved complete inhibition of biofilm formation. These results are similar to those obtained with chlorhexidine, the most commonly used oral antiseptic agent. The minimal inhibitory concentration of bismuth nanoparticles that interfered with S. mutans growth was 0.5 mM.

Conclusion: These results suggest that zerovalent bismuth nanoparticles could be an interesting antimicrobial agent to be incorporated into an oral antiseptic preparation.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJN.S29854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3356212PMC
September 2012

Detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis patients by multiplex PCR.

Acta Odontol Latinoam 2008 ;21(2):163-7

Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, México.

A Multiplex PCR assay for the detection of Porphyromonas gingivalis and Streptococcus intermedius in chronic periodontitis is presented. A total of 180 samples from 65 adults with untreated periodontitis and 17 healthy volunteers were taken and processed in a simple boiling step. Cell lysates were used as DNA source for multiplex PCR assays. Primers were designed from 16S rRNA gene sequences from the GenBank-EMBL database showing specificity for target pathogens. This multiplex PCR system could detect 8.2 P gingivalis and S. intermedius cells. In untreated periodontitis patients, only 78.5% were positive for one or both bacteria; 37% were positive for P gingivalis only, 17% for S. intermedius and 24.5% for both. P. gingivalis was detected in 23.5% of healthy volunteers, while S. intermedius was not detected in the same patients. The distribution of these bacteria was related to the periodontal probing depth, while 95.23% of patients with pockets wih 6 to 7 mm deep were positive for either or both, only 70.45% of of them with 4 to 5 mm pockets were positive.
View Article and Find Full Text PDF

Download full-text PDF

Source
April 2009
-->