Publications by authors named "Mykhailo S Myroshnychenko"

15 Publications

  • Page 1 of 1

MORPHOLOGICAL EFFICACY EVALUATION OF GEL WITH CARBON DIOXIDE EXTRACT OF HOPS IN CASE OF COMPLICATED WOUND INFECTION ACNE VULGARIS.

Wiad Lek 2021 ;74(1):112-117

KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.

Objective: The aim of the study is to reveal in the experiment the morphological features of the infected skin wounds healing, which are a manifestation of acne vulgaris severe and very severe forms, using a gel with carbon dioxide extract of hops.

Patients And Methods: Materials and methods: An experimental study was carried out on 80 male WAG rats of three months of age. The animals were divided into 9 groups. Group 1 consisted of intact animals (n=6). Group 2 was represented by animals (n=6), which had hair epilation on the dorsal surface of the body in an area of 1 сm2, followed by application of 2 ml of placebo gel to this area. Group 3 included animals (n=6), which were epilated on the dorsal surface of the body in an area of 1 сm2 and applied 2 ml of 1 % gel with carbon dioxide extract of hops. Group 4 included rats (n=6), which were epilated on the dorsal surface of the body in an area of 1 сm2 and simulated thermal damage. Group 5 was represented by 10 rats, who were epilated on the dorsal surface of the body in an area of 1 сm2, simulated thermal damage, followed by applying 2 ml of placebo gel to the wound surface. Group 6 included rats (n=10), who underwent measures similar to group 5, followed by application of 2 ml of 1 % gel with carbon dioxide extract of hops to the wound surface. In group 7, there were 6 rats, which were epilated on the dorsal surface of the body in an area of 1 сm2, thermal damage to the skin with underlying soft tissues was simulated, followed by application to the wound surface the reference strains of Staphylococcus aureus, Streptococcus pyogenes, Proteus vulgaris, Propionibacterium acnes, Escherichia coli, Pseudomonas aeruginosa, Malassezia slooffiae, Malassezia pachydermatis, Candida albicans, Candida parapsilosis. In groups 8 and 9, there were 15 rats each, which underwent measures similar to group 7, followed by applying 2 ml of placebo gel on its surface on the next day after infection of the wound in group 8, and in group 9 - 2 ml of 1 % gel with carbon dioxide extract hops. The material for the study was the skin with underlying soft tissues. It was used histological, histochemical, morphometric and statistical methods.

Results: Results: This experimentally created gel with carbon dioxide extract of hops activates separation processes of horny masses from the surface of the epidermis, cleaning the pores of the skin; stimulating the proliferative activity of the epidermis, which is located in the marginal sections of the wound or covers the surface of the regenerate; activating the processes of cleansing the wound from necrotic tissue; activating the growth and maturation of granulation tissue with its subsequent transformation into connective tissue. It has anti-inflammatory, bactericidal and antimycotic effects, normalizing skin microbiocenosis.

Conclusion: Conclusions: The complex morphological study has showed that gel with carbon dioxide extract of hops is a highly effective drug in treatment of severe and very severe acne vulgaris, characterized by the development of deep and infected wound defects.
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April 2021

IMMUNOHISTOCHEMICAL FEATURES OF THE EXPRESSION OF HUMAN PAPILLOMA VIRUS TYPE 16 IN PLEOMORPHIC ADENOMAS OF SALIVARY GLAND.

Wiad Lek 2021 ;74(1):7-10

NATIONAL CANCER INSTITUTE, KYIV, UKRAINE.

Objective: The aim is to reveal the immunohistochemical features of human papilloma virus type 16 expression in various histological variants of pleomorphic adenomas of the salivary gland.

Patients And Methods: Materials and methods: The material of the study was surgical and biopsy material from 30 patients with pleomorphic adenomas of the salivary glands, among which in 15 cases mesenchymal was detected, in 10 - mixed, in 5 cases - epithelial histological variant, respectively. Immunohistochemical study was performed, using mouse monoclonal antibody to human papilloma virus type 16. Visualization was performed, using an EnVisionTM FLEX detection system. Histological sections of grade III cervical intraepithelial neoplasia (CIN III) were used as a positive control; for a negative control, the procedure was performed without primary antibodies. The immunohistochemical reaction was assessed by a semi-quantitative method by counting the percentage of positively stained cells in the field of view of a microscope × 400. Microspecimens were studied, photoarchived on an Olympus BX-41 microscope.

Results: Results: Expression of human papilloma virus type 16 of varying severity was determined in 26 cases of pleomorphic adenomas of the salivary glands, which was 86.7%. The epithelial component of the pleomorphic adenoma of the salivary gland was characterized by a more pronounced expression of the monoclonal antibody to human papilloma virus type 16 compared to the mesenchymal component of the tumor. The severity of the immunohistochemical reaction with a monoclonal antibody to human papilloma virus type 16 depended on the histological variant of the pleomorphic adenoma of the salivary gland. Epithelial, mixed and mesenchymal variants of pleomorphic adenoma of the salivary gland were characterized, respectively, by the most pronounced, pronounced and moderately pronounced expression of a monoclonal antibody to human papilloma virus type 16.

Conclusion: Conclusions: A comprehensive immunohistochemical study with a monoclonal antibody to human papilloma virus type 16 revealed the presence of a causal relationship between the infection of a patient with human papilloma virus type 16 and development of pleomorphic adenoma of the salivary gland in him.
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April 2021

CLINICAL AND MORPHOLOGICAL CORRELATION DEPENDENCIES AND THEIR SIGNIFICANCE IN PATIENTS WITH COMPLICATIONS OF LABORED ERUPTION OF THE LOWER THIRD MOLARS.

Wiad Lek 2021 ;74(3 cz 1):441-449

KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.

Objective: The aim is to determine the clinical and morphological dependencies, which are important for diagnostics, treatment and prediction of outcomes of pathological processes in the region of the LTM with complicated eruption, as based on the study of histopathological changes of paradental tissue (mucous membrane, walls of retromolar pocket, alveolar bone tissue).

Patients And Methods: Materials and methods: The materials of the study were 34 biopsy specimens of pathologically altered soft tissue and parodontium obtained as a result of pericoronectomy, extraction of the LTM and other surgical interferences performed based on the relevant indications in 28 patients in the region of the LTM with complicated eruption. Morphological and statistical research methods were used.

Results: Results: The local pathological processes, which chronologically precede the destructive changes in the hard tissue of a tooth (caries), are developed in patients of both genders with complicated LTM eruption in soft tissue of parodontium and the adjacent bone tissue of the alveolar wall in the majority of cases. As per biopsy examinations, the frequency of the main pathological processes in paradental tissue in case of complicated LTM eruption varies from 25 to 60 % of the number of biopsy specimens and occurs in various combinations in patients with different values of clinical parameters. The correlation relationships between the patients' clinical data and the morphological parameters of damage to paradental tissue are weak, multidirectional and uncertain in the majority of combinations (considering the available number of biopsy specimens studied). The close certain positive dependence between the damage of the squamous epithelium and the inflammation activity in the lamina propria mucosae, covering the tooth: in the vast majority of cases, the presence of damaged epithelium (within the biopsy specimen) is associated with the inflammation of high activity, was established as based on correlation relationships between the morphological parameters of damage to paradental tissue.

Conclusion: Conclusions: The found pathological changes and the correlations justify surgical tactics on paradental soft and osseous tissues that are directed on the LTM sparing.
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April 2021

BAD HABITS AND THEIR IMPACT ON STUDENTS' HEALTH.

Wiad Lek 2020 ;73(11):2386-2395

ZHYTOMYR IVAN FRANKO STATE UNIVERSITY, ZHYTOMYR, UKRAINE.

Objective: The aim: Is to investigate the bad habits of the students of higher education institutions and their impact on health.

Patients And Methods: Materials and methods: The research of the students' bad habits was conducted at Polissia National University and Zhytomyr Ivan Franko State University in 2014-2019. 647 students of different specialties between the ages of 17 and 23 were interviewed. The research methods included theoretical methods (the analysis and generalization of literary sources, the evaluation of the quality of students' performance in classes, the study and analysis of the medical records of students); empirical methods (pedagogical observations, questionnaires, surveys); the methods of mathematical statistics.

Results: Results: The study found that 32.4 % of male students and 14.9 % of female students smoke. It was defined that male students of the special education department smoke the most - 37.7 %; 32.5 % and 27.1 % of the male students of the main and sports departments smoke respectively. Among female students, 18.4 % of the students of the sports department, 14.6 % - of the main department and 13.4 % - of the special department smoke. The study determined that 2.7 % of both male and female students of the first year of study drink alcohol almost every day, 3.7 % - 2-3 times a week, 18.2 % - once a week, 66.0 % - only on holidays. Besides, the research indicated that some students use drugs. It was found that the students know that smoking, alcohol, and drugs are harmful but cannot or do not want to get rid of these bad habits.

Conclusion: Conclusions: The study established that the environment of students is unfavorable for the activation of the healthy lifestyle components; it contributes to the spread of bad habits, the consequences of which students do not realize fully. Many students do not have a need to take care of their own health.
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January 2021

MORPHOLOGICAL CHARACTERISTICS OF PLEOMORPHIC ADENOMAS OF SALIVARY GLANDS (ANALYSIS OF THE SURGICAL MATERIAL).

Wiad Lek 2020 ;73(11):2339-2344

SHUPYK NATIONAL MEDICAL ACADEMY OF POSTGRADUATE EDUCATION, KYIV, UKRAINE.

Objective: The aim of the study is to reveal the morphological features of pleomorphic adenomas of the salivary glands during a comprehensive examination of the surgical material.

Patients And Methods: Materials and methods: Surgical material from 30 patients with pleomorphic adenomas of the salivary glands was studied. Microspecimens stained with hematoxylin and eosin were studied, using an Olympus BX-41 microscope (Japan) with subsequent processing with the Olympus DP-soft version 3.1 software, which was used to conduct a morphometric study. By morphometry in the tumor tissue, the specific volumes of the parenchyma and stroma, the thickness of the capsule located between the tumor tissue and the tissue of the salivary gland were determined; the absolute number of vessels in the field of view of the microscope was counted at × 100 magnification.

Results: Results: Comprehensive morphological analysis of the surgical material of removed neoplasms of the salivary glands has showed that mesenchymal (15 cases, 50.0%) and mixed (10 cases, 33.3%) variants of pleomorphic adenomas are more common, and less often epithelial variants (5 cases, 16.7%). Pleomorphic adenoma is characterized by a different ratio of the epithelial (parenchymal) and mesenchymal (stromal) components forming this tumor, structural diversity and heterogeneity of the structure of these components, which do not have clear boundaries and are mixed with each other. A characteristic feature of pleomorphic adenoma is also the combination in each case of different types of epithelial cells and the structures that they form, as well as areas of various differentiation of the mesenchymal component. Mesenchymal and mixed variants of pleomorphic adenomas, in comparison with the epithelial variant, are more prone to progression and recurrence, as evidenced by our identified active processes of angiogenesis in tumor tissue, frequent tumor invasion of the capsule, thinning of the capsule or the absence of the capsule, less pronounced infiltration of the capsule by immune cells.

Conclusion: Conclusions: The morphological features of mesenchymal, mixed and epithelial variants of pleomorphic adenomas of the salivary glands revealed by the authors should be takeninto account by clinicians during choosing the tactics for treating the patient, which will undoubtedly help to reduce the incidence of tumor malignization and its recurrence.
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January 2021

FEATURES OF LOCAL IMMUNE REACTIONS IN SKIN WITH UNDERLYING SOFT TISSUES IN PATIENTS WITH MULTIPLE SCLEROSIS.

Wiad Lek 2020 ;73(10):2109-2115

KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.

Objective: The aim of the study is to identify the peculiarities of local immune reactions in the skin with underlying soft tissues in patients with different variants of the multiple sclerosis' course.

Patients And Methods: Material and methods: The study included 35 patients, hospitalized in the neurological department of the Communal Nonprofit Enterprise of Kharkiv Regional Council «Regional Clinical Hospital» with the established diagnosis of multiple sclerosis. The patients were divided into three study groups, based on different variants of this pathology's course. Group 1 included 16 patients with relapsing-remitting type of multiple sclerosis. Group 2 included 11 patients with a secondary-progressive type of multiple sclerosis course. Group 3 included 8 patients with a primary progressive type of multiple sclerosis. Patients of all groups underwent a biopsy of the skin with underlying soft tissues in the lower third of the inner surface of the right lower leg. The comparison group (group 4) was represented by 10 autopsy cases (7 women and 3 men) conducted on the basis of the pathological anatomy department of the Communal Nonprofit Enterprise of Kharkiv Regional Council «Regional Clinical Hospital». There were no signs of the nervous system's pathology during life in all cases of this group. The cause of death was a dislocation of the brain stem or hematocephaly and the main disease was arteriovenous malformation or congenital aneurysm of the cerebral vessels. The material for the morphological study was skin with underlying soft tissues. Microspecimens stained with histological and immunohistochemical methods were studied, using an Olympus BX-41 microscope. The obtained data were statistically processed, using Statistica 6.0 and Microsoft Excel 2003 programs.

Results: Results: Survey microscopy showed that in groups 1-3 in comparison with group 4 immune cell infiltrations were more pronounced in the skin with underlying hypodermis. Significantly larger mean values of the absolute number of CD 3-, CD 20- and CD 68-positive cells were revealed immunohistochemically in groups 1-3 compared with group 4. Thus, it was found in patients with multiple sclerosis the activation of T-cell immunity, B-cell immunity and macrophage system with the development of an immune imbalance between them. Our results allow us to think about the participation of all the above immune cells in the pathogenesis of multiple sclerosis development. The revealed disorders of local immune reactions in the skin with underlying hypodermis in patients with multiple sclerosis are less pronounced in the remitting-recurrent variant of the course of the disease, more pronounced in the secondary-progressing and, especially, primary-progressing variants.

Conclusion: Conclusions: In patients with multiple sclerosis in the skin with underlying hypodermis activation of T-cell immunity, B-cell immunity and the macrophage system is observed with the development of an immune imbalance between them, characterized by the prevalence of the absolute number of macrophages among all immune cells. Less pronounced violations of local immune reactions in the skin with underlying hypodermis are noted in remitting-relapsing variant of multiple sclerosis course, more pronounced in a secondary-progressing and, especially, primary-progressing variants.
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December 2020

CHARACTERISTICS OF AUTOPSY MATERIAL IN CASES OF CHRONIC DIFFUSE PATHOLOGY OF THE LIVER PARENCHYMA DIAGNOSED DURING PATIENT'S LIFETIME USING THE ULTRASOUND METHOD.

Wiad Lek 2020 ;73(9 cz. 2):1995-1999

KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.

Objective: The aim: Of the study is to analyze autopsy cases where chronic diffuse pathology of the liver parenchyma was diagnosed during patient's lifetime using the ultrasound method.

Patients And Methods: Material and methods: In this study archival material (autopsy protocols and microspecimens of these cases) of the pathological anatomy department of the Communal Nonprofit Enterprise «Regional Clinical Hospital» (Kharkiv) was used for the period from 2012 to 2018. The cases diagnosed with a chronic diffuse pathology of the liver parenchyma according to ultrasound data were selected among all cases. Microspecimens stained with hematoxylin and eosin, picrofuxin according to van Gieson were examined using an Olympus BX-41 microscope (Japan). The obtained digital indicators were statistically processed using the programs Statistica 6.0, Microsoft Excel 2003. To compare the parameters, parametric and nonparametric methods were used (Student's t-test, χ2 criterion, Mann-Whitney U-test). Differences were considered significant at p<0.05.

Results: Results: According to the analysis of archival material for the period from 2012 to 2018, there was no change in the relative number of cases with fatty hepatosis, fibrosis or cirrhosis of the liver, chronic hepatitis, malignant tumors of the liver, metastatic liver damage and liver infiltration by leukemic cells, liver abscesses. Among all cases with revealed liver pathology, both separately by year and as a whole for the entire studied period, a predominance of the relative number of cases with fatty hepatosis, fibrosis or cirrhosis of the liver was noted.

Conclusion: Conclusions: The term «chronic diffuse pathology of the liver parenchyma», which is widely used in ultrasound diagnostics, is morphologically a collective term that combines pathological conditions such as fatty hepatosis, fibrosis or cirrhosis of the liver, chronic hepatitis, malignant tumors of the liver, metastatic liver damage and liver infiltration by leukemic cells, liver abscesses. The ultrasound term «chronic diffuse pathology of the liver parenchyma» needs to be improved as well as additional diagnostic criteria should be developed in order to correctly use it in practice and avoid diagnostic errors.
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November 2020

NITRIC OXIDE METABOLISM FEATURES UNDER CONDITIONS OF EXPERIMENTAL INFECTED RADIATION-INDUCED SKIN INJURIES DEVELOPMENT AND THEIR TREATMENT WITH PHOTODYNAMIC THERAPY.

Wiad Lek 2020 ;73(8):1655-1658

KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.

Objective: The aim: To follow-up nitric oxide content values in rat serum at the development of Staphylococcus aureus infected radiation skin injuries and their photodynamic therapy.

Patients And Methods: Materials and methods: Eighty WAG male rats were studied in an experiment. Four groups were identified for evaluation. Group 1 included unaffected intact rats (n=20). Group 2 involved rats (n=20) with a modeled radiation-induced ulcer of the skin. The rats (n=20) with a modeled radiation-induced skin ulcer followed by infecting with Staphylococcus aureus were referred to group 3. Group 4 included rats (n=20) with Staphylococcus aureus infected radiation skin ulcer exposed to photodynamic therapy. Rats of groups 1-4 were sampled for biochemical blood examination on days 7, 14, 21, 30 and 45. Total nitric oxide metabolites (nitrites and nitrates) were measured according to V.A. Metelskaya et al. method.

Results: Results: Infectious agent (Staphylococcus aureus) present in skin ulcer impairs nitric oxide metabolism in rat blood serum that manifested in decreased total nitric oxide metabolites content on day 7, followed by its increase within days 14 to 45. While photodynamic therapy exposed on the Staphylococcus aureus infected radiation skin ulcer, total nitric oxide metabolites in blood serum had increased by day 7, but days 14 to 45 level was compliant with physiological norm.

Conclusion: Conclusions: Infecting radiation skin ulcers with Staphylococcus aureus causes impaired nitric oxide metabolism, while photodynamic therapy helps to normalize the metabolism of the above-mentioned chemical compound that can improve healing of radiation skin ulcers.
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October 2020

Clinical and morphological features of the ovarian brenner tumour: current state of the problem.

Wiad Lek 2020 ;73(7):1420-1426

Public Non-Profit Organization Of The Izium City Council «Сentral City Hospital Of Sandy Mother Of God», Izium, Ukraine.

Objective: Introduction: Ovarian tumours are an actual problem of present-day medicine, being one of the most difficult sections of modern oncology. The majority of ovarian tumours are of epithelial origin. The ovarian Brenner tumour represents a rare epithelial ovarian neoplasm and accounts for 1-2% of all ovarian neoplasms. The aim of the study is to identify clinical and morphological features of ovarian Brenner tumour.

Patients And Methods: Materials and methods: The material was 5 cases of Brenner ovarian tumour, diagnosed in the study of 4 cases of operational material and 1 case of autopsy observation for the period from 2007 to 2019. Histological and immunohistochemical staining methods were used. The microspecimens were examined on an Olympus BX-41 microscope (Japan).

Results: Results: Ovarian Brenner tumour is a rather rare pathology, the histogenesis of which is debatable. Morphological examination is the main method for its diagnosing. Ovarian Brenner tumours developed in women of middle and old age (the average age was 51.8 years). Women with a malignant ovarian Brenner tumour were older than women with a benign variant (the average age in women with a malignant variant was 55.8 years, with a benign variant - 49.3 years). Benign ovarian Brenner tumour occurred more frequently compared with a malignant one. Malignant and benign variants of ovarian Brenner tumour were characterized by a one-sided nature of the lesion with frequent involvement in the pathological process of the left ovary. Clinically, in patients with a benign variant of the Brenner tumour in all cases an abdominal pain syndrome was determined, combined in one case with metrorrhagia. A malignant ovarian Brenner tumour was clinically manifested by severe abdominal pain syndrome, combined in one case with complaints of an increase in the size of the abdomen, and in another case with intoxication syndrome and a clinic of small bowel obstruction. In all cases a malignant ovarian Brenner tumour metastasized to the omentum and in one case also to the small intestine wall. Macroscopically the ovarian Brenner tumour had the form of a node, the dimensions of which were significantly larger for the malignant variant compared with a benign, dense or soft consistency, on the cross section of a whitish-gray or brown color with cysts. A damaged ovary with a malignant variant of Brenner tumour significantly increased in size, while with a benign one, its size did not change or increased slightly. In all cases the malignant and benign variants of ovarian Brenner tumour were combined with various reproductive system organs pathologies (mucinous papillary cystadenoma of the ovary, serous ovarian cyst, ovarian endometriosis, endometrial hyperplasia, cervical nabothian cysts, uterine leiomyoma).

Conclusion: Conclusions: A study conducted by the authors revealed clinical and morphological features of a rare ovarian tumour - Brenner tumour, which will contribute to a better understanding of this pathology by the doctors of various specialties, and improve the treatment and diagnostic process.
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August 2020

Morphological assessment of wound healing after cryodestruction of skin using an aqueous colloidal solution of C60 fullerenes.

Wiad Lek 2020 ;73(4):642-647

Kharkiv National Medical University, Kharkiv, Ukraine.

Objective: The aim is to assess the morphological state of a cold skin wound in an experiment, using an aqueous colloidal solution of C60 fullerenes.

Patients And Methods: Materials and methods: An experimental study was conducted in 30 six-month-old male rats divided into three groups, 10 individuals in each group: group I included rats that did not undergo any manipulations; group II was represented by rats which underwent a cold wound simulation on the lateral thigh surface; group III was represented by rats who had a simulated cold wound followed by introduction of an aqueous colloidal solution of C60 fullerenes at a dose of 0.1 mg/kg for 5 days into the abdominal cavity, starting from the cryodamage day. Animals of all groups were removed from the experiment on days 7, 14 and 21. The material for the morphological study was skin with underlying soft tissues. The resulting sample was studied, using histological, histochemical, morphometric and statistical research methods.

Results: Results: C60 fullerenes stimulate proliferative activity in the epidermis, located in the wound marginal regions or covering the regenerate surface, which leads to an increase in epithelial layer thickness by 2.6, 2.6, 2.5 times on 7, 14 and 21 days; promotes faster cleansing of the wound from necrotic tissue, reducing the thickness of the zone of primary necrosis on days 7, 14 and 21 by 1.3 times by increasing the demarcation zone on days 7, 14 and 21 by 1.4, 1.4 and 2, 2 times; reduces the thickness of the secondary necrosis area on 7, 14 and 21 days by 1.4, 1.5 and 1.3 times; accelerates filling of the wound defect with granulation tissue, the layer thickness of which increases by 2.3, 2.2 and 1.4 times on the 7th, 14th and 21st days, respectively; reduces the severity of hemodynamic disorders and cell infiltration in the wound defect adjacent tissues of the dermis, hypodermis with the underlying muscle tissue.

Conclusion: Conclusions: A comprehensive morphological study of the experimental material indicates that the injection into the abdominal cavity of an aqueous colloidal solution of C60 fullerenes activates reparative processes in the skin cold wounds, which go through all the classical stages.
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August 2020

Morphological features of a cold skin wound under the influence of an extract of cryopreserved skin fragments of piglets (experimental study).

Wiad Lek 2020 ;73(2):306-311

Kharkiv National Medical University, Kharkiv, Ukraine.

Objective: The aim of the study is to identify in an experiment the effect of an extract of cryopreserved fragments of piglets on the morphological state of a cold skin wound.

Patients And Methods: Materials and methods: Hairless six-month-old male rats were used in the study. They were divided into III groups: group I included 10 rats that had not been manipulated; group II was represented by 10 rats with cold wounds on the lateral surface of the thigh; group III was represented by 10 rats that were with a cold wound, followed by the injection of an extract of cryopreserved skin fragments of piglets into the abdominal cavity at a dose of 50 μg per 100 g of animal body weight (peptide concentration 100 μg/ml) once a day for 5 days from the time of wound modeling. Animals in groups I-III were withdrawn from the experiment on the 7th, 14th and 21st days. The material for themorphological study was the fragments of intact skin with underlying soft tissues from the thigh area in group I and the fragments of skin with underlying soft tissues from the thigh area directly from the zone of cryoexposure in groups II and III. Histological, histochemical and morphometric methods were used. Microspecimens were studied using an Olympus BX-41 microscope (Japan). Statistical processing was performed using the Statistica 6.0 and Microsoft Excel 2003 software package. Nonparametric methods were used to compare numerical values (Mann-Whitney U-test, Kruskal-Wallis test). The significance of differences between the average values of the indicators was taken at the level of p<0.05.

Results: Results: The extract of cryopreserved skin fragments of piglets has an effective wound healing effect compared to the healing processes in a cold wound, which was not subjected to any therapeutic effects. It was manifested in the improved process of cleansing the wound from necrotic tissues that entered the zone of primary necrosis, as evidenced by 1,2 times decrease of the zone of primary necrosis on the 7th, 14th and 21st days; a decrease of the zone of secondary necrosis on the 7th, 14th and 21st days, respectively, - 1.2, 1.3, 1.2 times; growth and maturation of granulation tissue activation, as evidenced by an increase in the thickness of a granulation tissue layer on 7, 14, 21 days, respectively, - 1.9, 1.8, 1.2 times; activation of proliferative processes in the epithelial layer located in the marginal sections of the wound defect or covering the regenerate surface, as evidenced by more pronounced acanthotic growths in the underlying tissue and an increase in the thickness of the epithelial layer on the 7th, 14th and 21st days, respectively, - 2.1, 2.0, 2.2 times.

Conclusion: Conclusion: The extract of cryopreserved skin fragments of piglets has an effective wound healing effect and can be recommended for further research in order to study the possibility of its use in clinical practice.
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May 2020

Clinical-morphological features of wilms’ tumour: analysis of literature data and a case from practice

Wiad Lek 2019 Oct;72(10):2050-2055

Military Medical Clinical Centre Of The Northern Region, Kharkiv, Ukraine

Wilms’ tumour, or nephroblastoma, is a malignant tumour, originating from nephrogenic germ cells that copy histology of developing kidneys and often contain cells at different stages of their differentiation. The article analyses clinical-morphological features of Wilms’ tumour, which is typical for childhood and seldom occurs in adults. The authors suppose that one of the causes of Wilms’ tumour development can be maternal complications, which arise during pregnancy, leading to inhibition of glomerulogenesis and tubulogenesis in the offspring kidneys, an increase the number of foci of primitive (immature) tissue, from which this tumour, as it is known, can originate. The described саse from practice of Wilms’ tumour is of particular interest because of an untypical age category, when the above pathology was diagnosed, demonstrates necessity of a multidisciplinary approach to its identification and necessitates its inclusion into the differential diagnostic line for the detection of kidneys tumours in adults.
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October 2019

[Effect of experimental acute postnatal and mixed hypoxia on the morphological state of the rats liver stromal component].

Wiad Lek 2018;71(8):1541-1549

Kharkov National Medical University, Kharkiv, Ukraine.

Objective: Introduction: In children population of Ukraine the diseases of the gastrointestinal tract are quite common, among which a significant number of cases occur in liver diseases. The complexity of the liver functions formation in antenatal and intranatal periods, its morphofunctional immaturity lead to the high sensitivity of this organ to the influence of various factors from the mother organism. Actual issues are the detection of the mother's pathology influence on the formation of diseases «cargo» in children remote stages of ontogenesis. In literature there are no data about the influence of hypoxia as the most common damaging factor in antenatal, intranatal and postnatal periods on the liver morphofunctional state of children of different ages, including its stromal component. The aim of the study was the detection of morphological features of the stromal component of the rats liver, which were in different terms of postnatal ontogenesis, exposed to acute postnatal and mixed hypoxia.

Patients And Methods: Materials and methods: The study material was the rats liver tissue. In this study three groups were formed: I, which included rats of WAG line, born to mothers with physiological pregnancy and derived from the experiment on the 1st, 14th and 35th days of postnatal ontogenesis; II, which included rats of WAG line, born to mothers with physiological pregnancy, who were exposed to high altitude hypoxia on the 1st day of postnatal ontogenesis and derived from the experiment on the 1st, 14th and 35th days of postnatal ontogenesis; III, which included rats of «Black hood» line, that developed under conditions of chronic intrauterine hypoxia due to the presence of arterial hypertension in their mothers, exposed to high altitude hypoxia on the 1st day of postnatal ontogenesis and derived from the experiment on the 1st, 14th and 35th days of postnatal ontogenesis. It was conducted the morphometrical study for determine the specific volume of the liver stroma and an immunohistochemical study using monoclonal antibodies to types I, III collagen and fibronectin.

Results: Results: Mixed experimental hypoxia leads to increase of the specific volume of liver stroma of descendants from the 1st to the 35th days of postnatal ontogenesis due to the sclerotic changes development, which manifest by thickening of the connective tissue fibers bundles, a decrease the distance between them, the presence of places among the connective tissue fibers with only collagen fibers, activation the collagen formation processes with the predominance of the mature type I collagen over the immature type III collagen, increase fibronectin expression. Acute postnatal hypoxia does not affect the specific volume of the liver stroma of descendants from the 1st to the 35th days, however since the 14th day leads to the sclerotic changes development in this organ, which increase to the 35th day, less pronounced compared with the detected sclerotic changes in thecase of mixed hypoxia, and are characterized by the thickening of the connective tissue fibers bundles with the subsequent decrease the distance between them, the expressed predominance of collagen fibers over elastic fibers, increased fibronectin expression, activation the collagen formation processes with the predominance of the type I collagen over the type III collagen.

Conclusion: Conclusions: Acute postnatal hypoxia from the 14th day of postnatal life and mixed hypoxia the 1st day lead to the sclerotic changes development in the children liver, which are more pronounced in cases of mixed hypoxia modeling and increase with the age.
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June 2019

[Morphometrical evaluation of fetuses and newborns kidneys status developing under maternal iron deficiency anemaia conditions].

Wiad Lek 2018;71(7):1222-1230

Kharkiv National Medical University, Kharkiv, Ukraine.

Objective: Introduction: Kidneys in fetuses and newborns play an important role in the homeostasis regulation and are the most vulnerable to the action of damaging factors from the mother organism, therefore it is important to timely assess their morphofunctional state. The aim: The purpose of the study was to reveal the morphometrical features of fetuses and newborns kidneys from mothers, whose pregnancy was complicated by iron deficiency anemia of varying degrees of severity.

Patients And Methods: Materials and methods: The material of the study was the tissue of kidneys of mature fetuses and newborns from mothers with physiological pregnancy (28 cases), as well as from mothers whose pregnancy was complicated by iron deficiency anemia of varying degrees of severity (85 cases). Morphometrical examination was performed during which the nephrogenic zone thickness, the specific volumes of renal corpuscles, tubules and stroma were determined. The absolute number of renal corpuscles, the specific volumes of immature and mature renal corpuscles and tubules were counted.

Results: Results: Maternal iron deficiency anemia of mild degree of severity in the offspring kidneys leads to an increase of the nephrogenic zone thickness; does not affect the absolute number of renal corpuscles; in fetuses in the cortical layer does not affect the specific volume of renal corpuscles, in the cortical and medullary layers decreases the specific volume of tubules and increases the specific volume of stroma, while in newborns in the cortical layer does not affect the specific volumes of renal corpuscles and tubules, increases the specific volume of stroma, in the medullary layer reduces the specific volume of tubules and increases the specific volume of stroma; increases the specific volumes of immature renal corpuscles, tubules and reduces the specific volumes of mature renal corpuscles, tubules. Maternal iron deficiency anemia of moderate and severe degree of severity in the offspring kidneys leads to an increase of the nephrogenic zone thickness; reduces the absolute number of renal corpuscles; decreases the specific volume of renal corpuscles in the cortical layer, reduces the specific volume of tubules and increases the specific volume of stroma in the cortical and medullary layers; increases the specific volumes of immature renal corpuscles, tubules and decreases the specific volumes of mature renal corpuscles and tubules.

Conclusion: Conclusions: Quantitative changes of the structural elements in the fetuses and newborns kidneys from mothers with iron deficiency anemia, characterized by a decrease of the parenchymal component with the presence a significant number of immature structures in it and an increase of the stromal component, will lead to kidneys dysfunction in such children.
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June 2019

[Morphological characteristics of kidneys connective tissue of mature fetuses and newborns from mothers, whose pregnancy was complicated by preeclampsia of varying degrees of severity].

Wiad Lek 2018;71(3 pt 1):579-587

Petro Mohyla Black Sea National University, Mykolaiv, Ukraine.

Objective: Introduction: The kidneys connective tissue condition in the antenatal period affects the formation of tissues and it changes with the development of various general pathological processes in this organ. The aim of the study was to identify the morphological features of kidneys connective tissue of fetuses and newborns from mothers whose pregnancy was complicated by preeclampsia of varying degrees of severity.

Patients And Methods: Materials and methods: The material of the study was the tissue of kidneys of mature fetuses and newborns from mothers with physiological pregnancy (28 cases), as well as from mothers whose pregnancy was complicated by preeclampsia of varying degrees of severity (78 cases). Immunohistochemical study was performed by an indirect Coons method according to M. Brosman's technique using monoclonal antibodies to collagen type I, III and IV.

Results: Results: The kidneys connective tissue of fetuses and newborns developing under the maternal preeclampsia conditions is characterized by the qualitative and quantitative changes that indicate the development of sclerotic processes in this organ, the severity of which increase with the age and with the increase of the maternal preeclampsia severity. Qualitative changes are characterized by an increase of the fibrous component, thickening of the bundles of connective tissue fibers, and a decrease in the distance between them. Quantitative changes are characterized by a pronounced predominance of collagen fibers over elastic fibers, almost total absence in some field of view elastic fibers and the violation of the content of collagen type I, III and IV.

Conclusion: Conclusion: Maternal preeclampsia underlies the development of qualitative and quantitative changes in kidneys connective tissue of fetuses and newborns, which as a result will lead to disruption of the functions of these organs in such children.
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August 2018