Publications by authors named "Myeong-Hyeon Wang"

101 Publications

Discovery and Functional Evaluation of Antimicrobials.

Antibiotics (Basel) 2021 Jun 23;10(7). Epub 2021 Jun 23.

Department of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Korea.

Microbial infections pose a continuous threat to human health and healthcare systems around the globe [...].
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http://dx.doi.org/10.3390/antibiotics10070765DOI Listing
June 2021

Chemical composition, antioxidant, and anti-diabetic activities of ethyl acetate fraction of Stachys riederi var. japonica (Miq.) in streptozotocin-induced type 2 diabetic mice.

Food Chem Toxicol 2021 Jun 26;155:112374. Epub 2021 Jun 26.

Department of Bio-Health Convergence, Kangwon National University, Chuncheon, 24341, Republic of Korea. Electronic address:

This work analysed the chemical composition, antioxidant, and enzyme inhibitory activities of solvent extract (SJ-ME) and fractions (SJ-HF, SJ-EAF, and SJ-MF) of the Stachys riederi var. japonica (Miq.) (SJ). Furthermore, the effect of SJ-EAF in STZ induced type 2 diabetic mice was examined. Among the samples, SJ-EAF exhibited a lower IC concentration of 64.2 ± 0.48 μg/mL for DPPH and 82.6 ± 0.09 μg/mL for ABTS. The SJ-EAF concentration of 2.89 ± 0.03 μg and 2.27 ± 0.98 μg was equivalent to 1 μg of acarbose mediated enzyme inhibitory effect against α-amylase and α -glucosidase, respectively. The SJ-EAF did not show cytotoxicity (<80%) to NIH3T3 nor HepG2 cells but enhanced the glucose uptake in the IR-HepG2. LC-MS/MS of SJ-EAF showed the presence of a total of 16 compounds. Among the identified compounds, rosmarinic acid, caffeic acid, oleanolic acid, and ursolic acid showed high catalytic activity of α-amylase and α-glucosidase. The treatments of SJ-EAF restored the level of blood glucose, body weight, insulin, HDL and mRNA level of IRS1, GLUT2, GLUT4 and Akt whereas it reduced the excess elevation of total cholesterol, total triglycerides, LDL, AST, ALT, ALP, BUN, and creatinine in STZ induced diabetic mice. Overall, the present study concluded that the SJ-EAF exhibited promising antidiabetic activity.
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http://dx.doi.org/10.1016/j.fct.2021.112374DOI Listing
June 2021

Antimicrobial and Wound Healing Properties of FeO Fabricated Chitosan/PVA Nanocomposite Sponge.

Antibiotics (Basel) 2021 May 3;10(5). Epub 2021 May 3.

Department of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Korea.

Diabetic and anemia-associated diabetic wounds increase the considerable morbidity and mortality in people, as reported by clinical studies. However, no anemia-associated diabetic wound dressing materials have been developed until now. Hence, this study aimed to develop a nanocomposite scaffold composed of chitosan (CS), poly (vinyl alcohol) (PVA), and phytogenic iron oxide nanoparticles (FeO NPs), for accelerated anemia-associated diabetic wound healing. The aqueous leaves extract of (PD) was utilized for the synthesis of iron oxide nanoparticles (FeO NPs). TEM and elemental analysis confirmed smaller size PD-FeO NPs (<50 nm) synthesis with the combination of iron and oxide. In addition, in vitro biological studies displayed the moderate antioxidant, antidiabetic activities, and considerable antibacterial activity of PD-FeO NPs. Further, the different concentrations of PD-FeO NPs (0.01, 0.03, and 0.05%) incorporated CS/PVA nanocomposites sponges were developed by the freeze-drying method. The porous structured morphology and the presence of PD-FeO NPs were observed under FE-SEM. Among nanocomposite sponges, PD-FeO NPs (0.01%) incorporated CS/PVA sponges were further chosen for the in vitro wound-healing assay, based on the porous and water sorption nature. Furthermore, the in vitro wound-healing assay revealed that PD-FeO NPs (0.01%) incorporated CS/PVA has significantly increased the cell proliferation in HEK293 cells. In conclusion, the CS/PVA-PD-FeO NPs (0.01%) sponge would be recommended for diabetic wound dressing after a detailed in vivo evaluation.
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http://dx.doi.org/10.3390/antibiotics10050524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147619PMC
May 2021

Synthesis, characterization, and cytotoxicity of starch-encapsulated biogenic silver nanoparticle and its improved anti-bacterial activity.

Int J Biol Macromol 2021 Jul 19;182:1409-1418. Epub 2021 May 19.

Department of Bio-Health Convergence, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address:

The present work reported synthesis, characterization, and biocompatibility of starch encapsulated silver nanoparticles (St-PF-AgNPs) and their antibacterial activity. The synthesis of St-PF-AgNPs involved in two steps: (i) synthesis of the biogenic silver nanoparticles using the fungal extracts (PF-AgNPs); and, (ii) encapsulation of starch in PF-AgNPs (St-PF-AgNPs). The surface plasmon resonance was found at 420 nm for the PF-AgNPs while it was at 260 and 420 nm for the St-PF-AgNPs. FTIR spectrum demonstrated the capping and encapsulation of the fungal extracts and starch in PF-AgNPs and St-PF-AgNPs. The XRD and TEM-EDS confirmed the crystalline nature, spherical-shaped , and polydispersed- PF-AgNPs and St-PF-AgNPs with strong signals of Ag. The St-PF-AgNPs showed a Z-average size of 115.2 d.nm and zeta potential of -17.8 (mV) as indicated by DLS and zeta potentials. The cytotoxicity results demonstrated higher toxicity of PF-AgNPs than St-PF-AgNPs in HEK293 cells. The antibacterial activity of St-PF-AgNPs were higher than PF-AgNPs in S. aureus. Overall, this work concluded that the starch encapsulation significantly increased the antibacterial activity of PF-AgNPs and this opens a new avenue for the treatment of bacterial infections through the sustained release of PF-AgNPs to target pathogenic bacterial cells.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.05.036DOI Listing
July 2021

pH-controlled nucleolin targeted release of dual drug from chitosan-gold based aptamer functionalized nano drug delivery system for improved glioblastoma treatment.

Carbohydr Polym 2021 Jun 5;262:117907. Epub 2021 Mar 5.

Department of Bio-Health Convergence, Kangwon National University, Chuncheon, 200-701, Republic of Korea. Electronic address:

This study developed the pH, and over-expressed nucleolin receptor responsive nano-drug delivery system (nDDS) composed by bio-synthesized gold nanoparticles (Au NPs), chitosan (CS) with aptamer (Apt) to deliver the 5-fluorouracil (5FU) and doxorubicin (Dox) for the improved glioblastoma treatment. The characterization results demonstrated that Apt-Dox-CS-Au-5FU NPs were monodispersed in nature with an average hydrodynamic particle size of 196.2 ± 2.89 nm and zeta potential of 16.26 ± 0.51 mV. The drug release, drug encapsulation efficiency (DEE), and loading efficiency (DLE) were measured by HPLC. The pH-responsive dual drug release was instigated the higher glioblastoma cell death instead of the single drug release through G/G phase cell cycle arrest. In addition, the internalization of Apt-Dox-CS-Au-5FU NPs in cell organelles was affirmed by bio-TEM analysis. Overall, this work revealed the newly designed drug-loaded smart nDDS improved the glioblastoma treatments.
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http://dx.doi.org/10.1016/j.carbpol.2021.117907DOI Listing
June 2021

Effects of yellow and red bell pepper (paprika) extracts on pathogenic microorganisms, cancerous cells and inhibition of survivin.

J Food Sci Technol 2021 Apr 5;58(4):1499-1510. Epub 2020 Sep 5.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, 200-701 South Korea.

The present work examined the biomedical value of red and yellow bell pepper extracts (YME and RME) in terms of antioxidant, antibacterial and anticancer activities by in vitro and virtual studies. The yield of extract was 3.49% for RME and 2.92% for YME. The level of total phenols and total flavonoids significantly varied between the type of extracts, and it was higher in RME than that in YME. The extracts showed promising DPPH and ABTS free radical scavenging rates. The extracts showed an excellent antibacterial activity. The minimal inhibitory concentration (MIC) of RME was 0.20 mg mL for , 0.30 mg mL for , 0.50 mg mL for and 0.60 mg mL and for , while the MIC of YME was 0.40 mg mL for 0.40 mg mL for , 0.50 mg mL for , and 0.60 mg mL for . TEM results demonstrated the cellular damage induced by RME in biofilm. The RME did not show any cytotoxicity in normal NIH3T3 cells, but at 125 μg mL did a strong cytotoxicity in human lung cancer cell line A549 as evident by cytotoxicity assay, ROS and AO/EB staining. The virtual biological examination indicated that β-carotene from RME was a potential compound with higher docking energy against both targeted enzymes and proteins as - 14.30 for LpxC and - 15.59 for survivin. Therefore, it is recommended that RME is a better functional food with novel biomedical properties and it deserves further evaluation for its the novel molecules against multidrug resistant pathogens.
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http://dx.doi.org/10.1007/s13197-020-04663-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925738PMC
April 2021

Metabolite Profiling of Methanolic Extract of by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities.

Pharmaceuticals (Basel) 2021 Jan 28;14(2). Epub 2021 Jan 28.

Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Korea.

In this study, the methanolic extract from seeds of exhibited strong antioxidant and enzyme inhibition activities with less toxicity to NIH3T3 and HepG2 cells at the concentration of 100 µg/mL. The antioxidant activities (DPPH and ABTS), α-amylase, and α-glucosidase inhibition activities were found higher in methanolic extract (MeOH-E) than HO extract. Besides, 9.82 ± 0.62 µg and 6.42 ± 0.26 µg of MeOH-E were equivalent to 1 µg ascorbic acid for ABTS and DPPH scavenging, respectively while 9.02 ± 0.25 µg and 6.52 ± 0.15 µg of MeOH-E were equivalent to 1 µg of acarbose for inhibition of α-amylase and α-glucosidase respectively. Moreover, the cell assay revealed that the addition of MeOH-E (12.5 µg/mL) increased about 37% of glucose uptake in insulin resistant (IR) HepG2 as compared to untreated IR HepG2 cells. The LC- MS/MS and GC-MS analysis of MeOH-E revealed a total of 54 compounds including terpenoids, glycosides, fatty acid, phenolic acid derivatives. Among the identified compounds, chlorogenic acid and jasminoside A were found promising for anti-diabetic activity revealed by molecular docking study and these molecules are deserving further purification and molecular analysis.
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http://dx.doi.org/10.3390/ph14020102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7912419PMC
January 2021

Ethyl Acetate Fraction of L. Induces Anti-Diabetic, and Wound-Healing Activities in Insulin-Resistant Human Liver Cancer and Mouse Fibroblast Cells.

Antioxidants (Basel) 2021 Jan 12;10(1). Epub 2021 Jan 12.

Department of Bio-health Convergence, Kangwon National University, Chuncheon 200-701, Korea.

Traditional, complementary, and integrative medicine are globally accepted alternative methods for the treatment of diabetes mellitus (DM). However, the mechanism of anti-diabetic effects of L. remains unproven. In the present study, antioxidant and anti-diabetic activity of the tubers of were studied in detail. Methanolic extracts of tubers were subjected to solvent fractionation method by increasing the polarity of the solvent using -hexane, and ethyl acetate. The obtained methanol extracts and its fractions were subjected to free radical scavenging activity (DPPH and ABTS assay) and in vitro enzyme (α-amylase and α-glucosidase) inhibition assay. Moreover, glucose uptake in insulin-resistant HepG2 cell line was analyzed. The preliminary phytochemical analysis confirmed the presence of phenolic and flavonoid compounds in the active fraction. The radical scavenging and in vitro diabetic related enzyme inhibitory activities were found to be dose dependent. The maximum ABTS and DPPH scavenging activity was documented in ethyl acetate fraction of the followed by methanol extract, hexane fraction, and methanol fraction. We also found that showed a less toxicity in mouse fibroblast cells and enhance the glucose uptake in insulin-resistant HepG2 cells. Besides, the ethyl acetate fraction of the analyzed by UPLC-QTOF-MS-MS and GC/MS revealed the presence of phenolic compounds such as neochlorogenic acid, chlorogenic acid, caffeic acid, 5--(4-coumaroyl)-quinic acid, feruloylquinic acid, caffeoylquinic acid, isoxazolidine, salicylic acid β-D-glucoside, dicaffeoylquinic acid isomers, salvianolic acid derivative isomers, and 1,4 dicaffeoylquinic acid etc. Among the identified phytochemicals, six were chosen for molecular docking study to explore their its inhibitory interactions with α-amylase and α-glucosidase. Taken together, the findings of the present study suggested that phytocompounds of EAF were responsible for the significant in vitro antioxidant, wound-healing, and anti-diabetic activities.
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http://dx.doi.org/10.3390/antiox10010099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7828129PMC
January 2021

Kenaf ( L.) Leaves and Seed as a Potential Source of the Bioactive Compounds: Effects of Various Extraction Solvents on Biological Properties.

Life (Basel) 2020 Sep 28;10(10). Epub 2020 Sep 28.

Department of Bio-Health Technology, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Korea.

(Kenaf) is a potential source of bioactive constituents and natural antioxidant. The current study determined the impact of various solvents on extraction yield, recovery of polyphenol and flavonoid, antioxidant, anticancer, and antibacterial properties of Kenaf leaves and seed. The powder of leaves and seed was separately extracted with -hexane, ethyl acetate, ethanol, and water solvent. Among them, the ethanol extract of leaves and seed showed the highest extraction yield, and their GC-MS analysis revealed a total of 55 and 14 bioactive compounds, respectively. The total polyphenols (TP) and flavonoids (TF) content were quantified by a spectrophotometric technique where water extracts displayed a noteworthy amount of TP and TF content compared to other extracts. A similar demonstration was noticed in antioxidant activity, evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydrogen peroxide scavenging capacity. In addition, cytotoxicity and anti-lung cancer activity were identified against mouse embryonic fibroblast (NIH3T3) and human lung cancer (A549) cells. All extracts of leaves and seed were observed as non-toxic to the NIH3T3 cells, but slight toxicity was expressed by -hexane extracts at the optimum dose (1000 µg/mL) of treatment. In parallel, -hexane and ethanol extracts (leaves and seed) exposed promising anti-lung cancer activity at the same concentration. Furthermore, antibacterial activity was assessed using disc diffusion assay, and seed extracts exhibited a significant inhibition zone against Gram-positive and Gram-negative microorganisms. Overall, Kenaf seed extracted with polar solvents was found very potent in terms of important bioactive compounds and pharmacological aspects, which can be an excellent biological matrix of natural antioxidants.
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http://dx.doi.org/10.3390/life10100223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601236PMC
September 2020

Chitosan nanoparticles as edible surface coating agent to preserve the fresh-cut bell pepper (Capsicum annuum L. var. grossum (L.) Sendt).

Int J Biol Macromol 2020 Dec 28;165(Pt A):948-957. Epub 2020 Sep 28.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea. Electronic address:

This work synthesized chitosan nanoparticles (CSNPs) by ionic gelation method using sodium tripolyphosphate pentabasic (STPP) and applied as nano-coating agent to extend the shelf life of fresh cut bell pepper (FCP) by preventing the microbial contaminations. The CSNPs were spherical shaped and 22.55 ± 1.69 d.nm sized with ζ-potential of 45.10 ± 1.42 mV confirmed by UHR-SEM, FE-TEM, and ζ-potential size analyses. The crystallinity and functional group changes were determined by XRD and FT-IR analyses respectivelly. The antioxidant activity of CSNPs was determined in terms of DPPH and ABTS scavenging activities. The 1% and 3% of CSNPs were found to be minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) respectively against all tested bacteria. The MBC of CSNPs was exhibited the promising anti-biofilm activity. Therefore, the 3% of CSNPs was used to prepare the chitosan nano-coating (CSNC), and applied on surface of FCP to prevent the microbial contaminations such as fungi, bacteria including Listeria monocytogenes and Salmonella enterica. The experimental results showed that the application of CSNC was maintained the FCP for 12 days at 5 °C without loss of weight, and sensory quality.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.09.176DOI Listing
December 2020

Synthesis and characterization of nano-chitosan capped gold nanoparticles with multifunctional bioactive properties.

Int J Biol Macromol 2020 Dec 24;165(Pt A):747-757. Epub 2020 Sep 24.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea. Electronic address:

The present work synthesized nano-chitosan capped gold nanoparticles (CS-AuNPs) and tested its bioactivities. The fourier-transform infrared spectroscopy (FTIR) exhibited glucosamine group revealed the capping of chitosan in CS-AuNPs. The X-ray photoelectron spectroscopy (XPS) demonstrated the peaks at 84.49 eV and 88.49 eV was corresponding to gold. The dynamic light scattering (DLS) analysis indicated the size of 98.14 ± 6.62 nm, poly dispersion index (PDI) of 0.232 and zeta potential of 46.8 ± 2.07 mV for CS-AuNPs. The CS-AuNPs was exhibited the higher antioxidant, antibacterial and diabetes related enzyme inhibitory activities than that in CSNPs. The 50% of LN229 cell death was occurred through the treatment of 218.75 μg. mL and 500 μg. mL of CS-AuNPs and CSNPs respectively. The inhibitory concentration (IC) of CS-AuNPs showed higher cytotoxicity in LN229 via cellular damage, reactive oxygen species (ROS), and arresting about 42.33% cells in the G phase. These results proved the multifunctional bioactive properties of CS-AuNPs.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.09.177DOI Listing
December 2020

GG and Biochemical Agents Enrich the Shelf Life of Fresh-Cut Bell Pepper ( L. var. grossum (L.) Sendt).

Foods 2020 Sep 7;9(9). Epub 2020 Sep 7.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Korea.

This work analyzed the individual and combined effects of biochemical additives and probiotic strain GG on red and yellow fresh-cut bell pepper (R- and Y-FCBP, respectively) stored at two different temperatures (4 °C and 15 °C) for 15 days. The results revealed that the combined application of biochemical additives and GG inhibited the colonization of total bacterial counts (25.10%), total counts (38.32%), total counts (23.75%), and total fungal counts (61.90%) in FCBP. Total bacterial colonization was found to be higher in R-FCBP (1188.09 ± 9.25 CFU g) than Y-FCBP (863.96 ± 7.21 CFU g). The storage at 4 °C was prevented 35.38% of microbial colonization in FCBP. Importantly, the GG count remained for up to 12 days. Moreover, the combined inoculation of the biochemical additives and GG treatments (T3) maintained the quality of R- and Y-FCBP for up to 12 days at 4 °C without any loss of antioxidant properties. This work reports the successful utilization of GG as a preservative agent for maintaining the quality of FCBP by preventing microbial colonization.
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http://dx.doi.org/10.3390/foods9091252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7555232PMC
September 2020

Folic acid functionalized starch encapsulated green synthesized copper oxide nanoparticles for targeted drug delivery in breast cancer therapy.

Int J Biol Macromol 2020 Dec 9;164:2073-2084. Epub 2020 Aug 9.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address:

This work aimed to synthesis copper oxide nanoparticles (CuONPs) using the Helianthus tuberosus (Ht) extracts then encapsulated with starch (ST) followed by conjugated with folic acid (FA) to facilitate the targeted release in MDA-MB-231 cells and this nanoparticles (NPs) was named as FA-ST-HtCuONPs. The TEM and DLS revealed that the FA-ST-HtCuONPs was hexagonal, oval-shaped with size of ~108.83 nm, and zeta potential of 43.26 mV. FTIR analysis indicated the presence of functional derivatives related to starch, folic acid and phytomolecules in NPs. Besides, the about 241.25 nmol/mg of folic accumulation on surface of the FA-ST-HtCuONPs were confirmed by UV-visible spectroscopic method. The cytotoxicity results revealed that among the samples, the inhibitory concentration (IC) of FA-ST-HtCuONPs (21.03 ± 1.85 μg/mL) was exhibited higher cytotoxicity to human breast cancer (MDA-MB-231) cells through activating reactive oxygen species (ROS) generation, nuclear damage and reduction of mitochondrial membrane potential and activating the apoptosis-related protein expression. Overall, the results proved that folic acid and starch decoration were increased the NPs penetration in cell through folate receptor-based endocytosis for enhanced breast cancer therapy.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.036DOI Listing
December 2020

Preparation, characterization and anti-cancer activity of graphene oxide-‑silver nanocomposite.

J Photochem Photobiol B 2020 Sep 30;210:111984. Epub 2020 Jul 30.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address:

This work reported the preparation, characterization, cytotoxicity of green synthesized Lespedeza cuneate mediated silver nanoparticles (Lc-AgNPs) and graphene oxide‑silver nanocomposite (GO-AgNComp) using Lc-AgNPs. The UV absorption spectrum at 419 nm indicated the successful formation of GO-AgNComp. The TEM analysis displayed the thin sheet of graphene decorated Lc-AgNPs in GO-AgNComp. Zeta potential was -13.2 mV for Lc-AgNPs and -30.5 mV for GO-AgNComp. The photothermal conversion efficiency was calculated as 31.09% for GO-AgNComp. The negatively charged zeta potential of GO-AgNComp enhanced its cellular penetration through enhanced permeability and retention (EPR) effect. The near-infrared laser (NIR) induced the anticancer activity of Lc-AgNPs and GO-AgNComp in human lung cancer cells (A549) and brain tumour (LN229). The results indicated that about 50% of A549 cells and LN229 cells were ablated by treatment of 24.73 ± 2.98 μg/mL and 27.34 ± 1.62 μg/mL of Lc-AgNPs, as well by 15.46 ± 2.31 μg/mL and 20.95 ± 1.35 μg/mL of GO-AgNComp respectively. Moreover, GO-AgNComp was not cytotoxic to normal mouse fibroblast cells (NIH3T3), but it caused the cancer cell death in A549 and LN229 through ROS generation, nuclear damage, and mitochondrial membrane potential (∆ψm) loss. This work reported the anticancer potential of GO-AgNComp, which deserves further study on the molecular elucidation of GO-AgNComp mediated human lung and tumour therapy.
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http://dx.doi.org/10.1016/j.jphotobiol.2020.111984DOI Listing
September 2020

Dual stimuli-responsive release of aptamer AS1411 decorated erlotinib loaded chitosan nanoparticles for non-small-cell lung carcinoma therapy.

Carbohydr Polym 2020 Oct 20;245:116407. Epub 2020 May 20.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, 200-701, South Korea. Electronic address:

The present work was developed the pH dependent-aptamer AS1411 (APT) decorated and erlotinib (En) loaded chitosan nanoparticles (CSNPs) for promising non-small-cell lung carcinoma (NSCLC) treatment. The characterization studies revealed that formulated APT-En-CSNPs were spherical in shape with size of 165.95 d. nm and PDI of 0.212. FTIR spectrum recorded molecular chemical interactions with composition of En or En-CSNPs. Cell viability assay, flow cytometry and fluorescent microscopy results revealed that APT-En-CSNPs triggered cancer cell death through pH-sensitive and nucleolin receptor-targeted release of En. The decoration of the APT improved the cellular uptake of En as evidenced by cellular sensing fluorescence and BioTEM assay. The APT-En-CSNPs induced the apoptosis through excessive ROS generation, nucleus damage and Δψm loss in the A549 cells. Hence, the present study revealed that the APT-En-CSNPs improved the therapeutic efficiency of En in NSCLC through the nucleolin targeted drug release.
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http://dx.doi.org/10.1016/j.carbpol.2020.116407DOI Listing
October 2020

Trigonelline-loaded chitosan nanoparticles prompted antitumor activity on glioma cells and biocompatibility with pheochromocytoma cells.

Int J Biol Macromol 2020 Nov 22;163:36-43. Epub 2020 Jun 22.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address:

Trigonelline-loaded water-soluble chitosan nanoparticles (Trigo-WSCS NPs) were prepared for the treatment of glioblastoma (targeting C6 glioma cells) and also evaluated its biocompatibility with rat adrenal pheochromocytoma cells (PC12 cells). WSCS-Trigo NPs characteristics were determined using UV-Visible spectrophotometer, FTIR, XRD, TEM, DLS, and Zeta potential. Trigo-WSCS NPs were noted to have a spherical shape, with an average size of 356 nm. Trigo-WSCS NPs zeta potential was 30.9 mv, which expresses its good stability. The WSCS-Trigo NPs considerably inhibited the growth of rat C6 glioma cells and exhibited an IC concentration of 34 μg/mL. Further, Trigo-WSCS NPs were biocompatible with PC12 cells in terms of enhancing neurite growth and differentiation. In conclusion, Trigo-WSCS NPs could act as an antitumor drug for the treatment of glioblastoma as suggested by the in vitro studies.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.165DOI Listing
November 2020

An effective datasets describing antimicrobial peptide produced from - purification and mode of action determined by molecular docking.

Data Brief 2020 Aug 22;31:105745. Epub 2020 May 22.

Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Korea.

Most of the probiotics Bacterial cells, express native antibacterial genes, resulting in the production of, antimicrobial peptides, which have various applications in biotechnology and drug development. But the identification of antibacterial peptide, structural characterization of antimicrobial peptide and prediction on mode of action. Regardless of the significance of protein manufacturing, three individual factors are required for the production method: gene expression, stabilization and specific peptide purification. Our protocol describes a straightforward technique of detecting and characterizing particular extracellular peptides and enhancing the antimicrobial peptide expression we optimized using low molecular weight peptides. This protocol can be used to improve peptide detection and expression. The following are the benefits of this method, (DOI - https://doi.org/10.1016/j.ijbiomac.2019.10.196 [1]). The data briefly describe a simple method in detection identification, characterization of antimicrobial extracellular peptide, predicating the mode of action of peptide in targeting pathogens ( method), brief method on profiling of antimicrobial peptide and its mode of action [1]. Further the protocol can be used to enhance the specific peptide expressions, detection of peptides. The advantages of this technique are presented below:•Characterization protocol of specific antimicrobial peptide•The folded antimicrobial peptide expression were less expressed or non-expressed peptides.•Besides being low cost, less time-consuming, easy to handle, universal and fast to execute, the suggested technique can be used for multiple proteins expressed in probiotics () expression system.
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http://dx.doi.org/10.1016/j.dib.2020.105745DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7287260PMC
August 2020

Bioactive Potential of 2-Methoxy-4-vinylphenol and Benzofuran from L. var. (Red Cabbage) on Oxidative and Microbiological Stability of Beef Meat.

Foods 2020 May 4;9(5). Epub 2020 May 4.

Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.

In the future, plant based phytochemicals will be considered as efficient replacement sources of chemical preservatives, to act as potential bio-preservatives. We investigated the antibacterial and antioxidant activity of red cabbage (RC) extracts using different solvents. Among all extracts, chloroform extract exhibited strong antimicrobial and antioxidant activities. Hence, the phytochemical constitutions of the RC chloroform extract was examined by GC-MS analysis, and further, based on molecular docking analysis, revealed 2-Methoxy-4-vinylphenol and benzofuran as two major compounds found to be possessing higher degrees of interaction with DNA gyrase (4PLB; -8.63 Kcal.mol) and lipoprotein (LpxC-8.229 Kcal.mol), respectively, of the bacterial cell wall, which leads to higher antimicrobial efficacy. Further, it was confirmed with that the in vivo model (but no cytotoxic effect) was exhibited in the MCF-7 cell line. Thus, we investigated the influence of this extract on the shelf life of meat under refrigeration storage. The physicochemical properties were observed periodically, and microbial analysis was conducted. The shelf life of the beef was enhanced (up to eight days) in terms of microbial and physiochemical properties, at 4 ± 2 °C when compared to control. We concluded that chloroform extract of RC has potential as a natural preservative in the meat processing industry.
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http://dx.doi.org/10.3390/foods9050568DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7278745PMC
May 2020

Simple and cleaner system of silver nanoparticle synthesis using kenaf seed and revealing its anticancer and antimicrobial potential.

Nanotechnology 2020 Apr 6;31(26):265101. Epub 2020 Mar 6.

Department of Bio-Health Technology, College of Biomedical Science, Kangwon National University, 1 Kangwon daehakgil, Chuncheon-si, Kangwon-do 24341, Republic of Korea. Institute of Kenaf Co., Ltd., Chuncheon 24341, Republic of Korea.

The clean and eco-friendly synthesis of silver nanoparticles (AgNPs) has provided promising characteristics with impressive biomedical related potential. Here, we have employed a green process for the synthesis of AgNPs using kenaf seed (KS) extract as a bilateral mediator for reducing and capping of Ag ions under hydrothermal condition. The synthesis pathways, such as varying amounts of KS, Ag ion concentration and autoclaving time were optimized. The manifestation of a strong absorption peak from 420-430 nm in UV-vis spectroscopy indicated the successful synthesis of [email protected] Fourier transform infrared spectroscopy confirmed the presence of hydroxyl and carbonyl functionalities involved in the reduction and stabilization of Ag ions. Furthermore, transmission electron microscopy revealed that the [email protected] are spherical in shape having a size around 7-11 nm, whereas high-quality crystals were evidenced by x-ray diffraction analysis. Moreover, inductively coupled plasma-optical emission spectrometry revealed that 19.6 μg l of Ag ions were released from the [email protected] In cell line studies, [email protected] at a higher dose were shown to be non-toxic to the healthy (NIH3T3) cells, while strong anti-proliferative response was found in the case of lung cancer (A549) cells. Furthermore, a significant zone of inhibition was observed for both Gram-positive and Gram-negative microorganisms, and a combination of [email protected] with ampicillin revealed a notable synergistic anti-pathogenic effect. Overall, our study proved the potentiality of KS as an efficient bio-resource for the synthesis of AgNPs and also its original feature as an anti-cancer and antimicrobial agent.
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http://dx.doi.org/10.1088/1361-6528/ab7d72DOI Listing
April 2020

Biocompatible fungal chitosan encapsulated phytogenic silver nanoparticles enhanced antidiabetic, antioxidant and antibacterial activity.

Int J Biol Macromol 2020 Jun 26;153:63-71. Epub 2020 Feb 26.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon 24341 Republic of Korea. Electronic address:

The work synthesized the fungal chitosan (FCS) encapsulated Gynura procumbens (GP) mediated silver nanoparticles (GP-AgNPs) for enhanced antidiabetic, antioxidant and antibacterial activity. The FCS-GP-AgNPs were characterized through UV-Visible spectroscopy, FTIR, XRD, Zeta size analyzer and TEM. The FTIR spectrum of GP-AgNPs exhibited functional groups of phenolic and flavonoids. The crystal peaks related to silver and chitosan in FCS-GP-AgNPs were demonstrated by XRD spectrum. The polydispersed nanoparticles such as AgNPs and FCS-GP-AgNPs were observed with size <100 nm by TEM. Zeta potential size analyzer indicated the average size and zeta potential of GP-AgNPs were 78.37 nm and -32.9 mV, whereas FCS-GP-AgNPs had 53.6 mV and 79.65 nm respectively. The FCS-GP-AgNPs was inhibited the α-glucosidase and α-amylase at 3.6 and 7.5 μg/mL respectively. Furthermore, FCS-GP-AgNPs were showed minimal inhibitory concentration (MIC) for Bacillus cereus (8.12 ± 0.12 μg/mL), Staphylococcus aureus (4.08 ± 0.47 μg/mL), Listeria monocytogenes (4.95 ± 0.32 μg/mL), Escherichia coli (8.25 ± 0.18 μg/mL), and Salmonella enterica (4.12 ± 0.64 μg/mL). In addition, the biocompatibility of FCS-GP-AgNPs was tested in A549, LN229, and NIH3T3 cells. This work concluded that FCS-GP-AgNPs proved to be biocompatible in terms of less cytotoxicity and promising in antibacterial and diabetics related enzyme inhibitory activity.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.02.291DOI Listing
June 2020

Physical and bioactivities of biopolymeric films incorporated with cellulose, sodium alginate and copper oxide nanoparticles for food packaging application.

Int J Biol Macromol 2020 Jun 24;153:207-214. Epub 2020 Feb 24.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea. Electronic address:

The present work prepared the antibacterial polymeric film (APF) using the different combination of sodium alginate (SA) and cellulose nano whisker (CNW) embedded with copper oxide nanoparticles (CuO NPs). Here, the SA acts as a plasticizer and provides flexibility. The CNW improves barrier properties of the film to limit moisture penetration into food. CuO NPs prevent the microbial contamination to food. Cross-linking and functional relationship of SA-CNW-CuNPs film were confirmed by adopting characterization including SEM, FTIR, EDS and XRD. The film composed with CNW (0.5%)-SA (3%)-CuNPs (5 mM) exhibited promising antibacterial activity against several pathogens in terms of higher zone of inhibition against S. aureus (27.49 ± 0.91 mm), E. coli (12.12 ± 0.58 mm), Salmonella sp. (25.21 ± 1.05 mm), C. albicans (23.35 ± 0.45 mm) and Trichoderma spp. (5.31 ± 1.16 mm). In addition, CNW (0.5%)-SA(3%)-CuO NPs (5 mM) film showed the challenging antioxidant activity in terms of DPPH and ABTS scavenging. Also, the optimized composition of film composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) was preventing the microbial contamination in fresh cut pepper (FCP). Therefore, APF composed with CNW (0.5%)-SA (3%)-CuO NPs (5 mM) is proved to be an active food packaging system (FPS) for its utility in food industry to overcome the limitations in conventional food packaging.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.02.250DOI Listing
June 2020

Enhanced anti-lung carcinoma and anti-biofilm activity of fungal molecules mediated biogenic zinc oxide nanoparticles conjugated with β-D-glucan from barley.

J Photochem Photobiol B 2020 Jan 3;203:111728. Epub 2019 Dec 3.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address:

This work reports the optimization, synthesis, characterization, anticancer, and antibacterial activity of the Trichoderma-β-D-glucan‑zinc oxide nanoparticles (T-β-D-glu-ZnO NPs). Firstly, the T-ZnO NPs was synthesized using the fungal mycellial water extract (FWME) derived from T. harzianum (SKCGW009) under the optimized condition of extract concentration (5.99 mL), temperature (43.11 °C), pH (8) and time (69.04 h). The successful conjugation of T-ZnO NPs with β-D-glucan (T-β-D-glu-ZnO NPs) was confirmed by PACE and FTIR. The XRD, UHR SEM, and TEM EDS results pointed the spherical shape of NPs with the mean size of 30.34 nm. Further, the XPS survey scan and high-resolution fitting of Zn2p results also claimed the successful formation of the T-β-D-glu-ZnO NPs. Cytotoxicity results indicated that the NPs were not toxic to NIH3T3 cells, while exhibited the dose-dependent inhibitory effect to human pulmonary carcinoma A549 cells. The IC of T-ZnO NPs and T-β-D-glu-ZnO NPs against A549 cells was 158 and 56.25 μg.mL, respectively, which was also verified by fluorescent cytochemistry. Annexin V-FITC staining results indicated the presence of apoptotic cells in the NPs treated A549 cells, which was not seen in the non-treated control A549 cells. Interestingly, the number of necrosis cells was higher in the T-ZnO NPs (3.38%) comparing to T-β-D-glu -ZnO NPs (0.07%). The early or late apoptosis was found higher in the cells treated T-β-D-glu -ZnO NPs (6.43%) comparing with T-ZnO NPs (4%). These results indicated that T-ZnO NPs and T-β-D-glu-ZnO NPs induced the cancer cell death through necrosis and apoptosis pathway, respectively. The antibacterial results indicated that the NPs treatment were significantly inhibited the growth of the Staphylococcus aureus inside of roundworm and enhanced growth of roundworm. Overall, anticancer and in vitro, in vivo antibacterial studies proved the high caliber of T-β-D-glu-ZnO NPs for the further pharmaceutical evaluation.
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http://dx.doi.org/10.1016/j.jphotobiol.2019.111728DOI Listing
January 2020

Biopolymer K-carrageenan wrapped ZnO nanoparticles as drug delivery vehicles for anti MRSA therapy.

Int J Biol Macromol 2020 Feb 7;144:9-18. Epub 2019 Dec 7.

Natural Product Biomedical Technology Laboratory, Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea. Electronic address:

Kappa-Carrageenan wrapped ZnO nanoparticles (KC-ZnO NPs) was synthesized, physico-chemically characterized and evaluated their biocompatibility and antimicrobial therapy against MRSA. XRD showed the highly crystalline and hexagonal phase structure of ZnO NPs. FETEM confirmed the spherical and hexagonal shaped particle with the mean size of 97.03 ± 9.05 nm. The synthesized KC-ZnO NPs exhibited significant antibacterial activity against MRSA. The biofilm growth of MRSA was greatly inhibited at 100 μg/ml as observed through live and dead cell assay. KC-ZnO NPs have shown invitro anti-inflammatory activity (82%) at 500 μg/ml. KC-ZnO NPs was non-toxic to NIH3T3 mouse embryonic fibroblasts cell lines. Further, no apoptotic and necrotic mediated death in NIH3T3 mouse embryonic fibroblasts cells were noticed by flow cytometric analysis. KC-ZnO NPs have good biocompatibility as recorded by the least hemolysis percentage (<3%) up to 100 μg/ml, which is much lesser than the acceptable limit. In addition, ecosafety analysis has shown that KC-ZnO NPs and kappa karrageenan (0-500 μg/ml) caused no mortality of A. salina after 48 h. However, bare zinc acetate has shown 35% mortality of A. salina after 48 h. The results conclude that KC-ZnO NPs could be a novel antibacterial therapy for the treatment of MRSA associated infectious.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.12.030DOI Listing
February 2020

Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model.

Int J Biol Macromol 2020 Jan 27;143:555-572. Epub 2019 Nov 27.

Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, South Korea. Electronic address:

Human-milk-based probiotics play a major role in the early colonization and protection of infants against gastrointestinal infection. We investigated potential probiotics in human milk. Among 41 Lactic acid bacteria (LAB) strains, four strains showed high antimicrobial activity against Escherichia coli 0157:H7, Listeria monocytogenes ATCC 15313, Bacillus cereus ATCC 14576, Staphylococcus aureus ATCC 19095, and Helicobacter pylori. The selected LAB strains were tested in simulated gastrointestinal conditions for their survival. Four LAB strains showed high resistance to pepsin (82%-99%), bile with pancreatine stability (96%-100%), and low pH (80%-94%). They showed moderate cell surface hydrophobicity (22%-46%), auto-aggregation abilities (12%-34%), and 70%-80% co-aggregation abilities against L. monocytogenes ATCC 15313, S. aureus ATCC 19095, B. cereus ATCC 14576, and E. coli 0157:H7. All four selected isolates were resistant to gentamicin, imipenem, novobiocin, tetracycline, clindamycin, meropenem, ampicillin, and penicillin. The results show that Pediococcus acidilatici is likely an efficient probiotic strain to produce < 3 Kda pediocin-based antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography mass spectrometry and HPLC with the corresponding sequences from class 2 bacteriocin, and based on the molecular docking, the mode of action of pediocin was determined on LipoX complex, further the C nuclear magnetic resonance structural analysis, which confirmed the antimicrobial peptide as pediocin.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.10.196DOI Listing
January 2020

Emerging Strategies in Stimuli-Responsive Nanocarriers as the Drug Delivery System for Enhanced Cancer Therapy.

Curr Pharm Des 2019 ;25(24):2609-2625

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon, 24341, Korea.

The conventional Drug Delivery System (DDS) has limitations such as leakage of the drug, toxicity to normal cells and loss of drug efficiency, while the stimuli-responsive DDS is non-toxic to cells, avoiding the leakage and degradation of the drug because of its targeted drug delivery to the pathological site. Thus nanomaterial chemistry enables - the development of smart stimuli-responsive DDS over the conventional DDS. Stimuliresponsive DDS ensures spatial or temporal, on-demand drug delivery to the targeted cancer cells. The DDS is engineered by using the organic (synthetic polymers, liposomes, peptides, aptamer, micelles, dendrimers) and inorganic (zinc oxide, gold, magnetic, quantum dots, metal oxides) materials. Principally, these nanocarriers release the drug at the targeted cells in response to external and internal stimuli such as temperature, light, ultrasound and magnetic field, pH value, redox potential (glutathione), and enzyme. The multi-stimuli responsive DDS is more promising than the single stimuli-responsive DDS in cancer therapy, and it extensively increases drug release and accumulation in the targeted cancer cells, resulting in better tumor cell ablation. In this regard, a handful of multi-stimuli responsive DDS is in clinical trials for further approval. A comprehensive review is crucial for addressing the existing knowledge about multi-stimuli responsive DDS, and hence, we summarized the emerging strategies in tailored ligand functionalized stimuli-responsive nanocarriers as the DDS for cancer therapies.
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http://dx.doi.org/10.2174/1381612825666190709221141DOI Listing
April 2020

Garlic clove extract assisted silver nanoparticle - Antibacterial, antibiofilm, antihelminthic, anti-inflammatory, anticancer and ecotoxicity assessment.

J Photochem Photobiol B 2019 Sep 17;198:111558. Epub 2019 Jul 17.

Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science campus 6th floor, Alagappa University, Karaikudi 630004, Tamil Nadu, India. Electronic address:

Facile and low cost garlic clove extract based silver nanoparticles was synthesized and its broad spectrum of therapeutic activity including antibiofilm, antiparasitic and anti-breast cancer activity was evaluated. The synthesized garlic‑silver nanoparticles (G-AgNPs) were characterized by various physico-chemical techniques. G-AgNPs showed good optical property, highly crystalline nature, spherical shape and uniformly dispersed with size measuring between 10 and 50 nm. G-AgNPs have shown greater anti-bacterial and antibiofilm activity on clinically important pathogens methicillin-resistant S. aureus and P. aerigunosa at 100 μg ml. The efficacy of G-AgNPs against earthworm evidenced its effectiveness as anti-helminthic agent in treating intestinal parasites. The significant inhibition of BSA protein denaturation proves its anti-inflammatory property. In addition, G-AgNPs have shown remarkable anticancer effect and significantly inhibited the human breast cancer cell (MCF-7) viability at 100 μg ml after 24 h. A noticeable change in the morphology of MCF-7 cells was also noticed. G-AgNPs were non-toxic to human HEK293 embryonic cells. Also, the non-toxic nature of G-AgNPs to C. cornuta and no morphological, physiological changes proved its safety to the environment. It is concluded that G-AgNPs have a broad range of biological applications and it can be used as an eco-friendly material without having negative effects in the environment.
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http://dx.doi.org/10.1016/j.jphotobiol.2019.111558DOI Listing
September 2019

Enhanced cancer therapy with pH-dependent and aptamer functionalized doxorubicin loaded polymeric (poly D, L-lactic-co-glycolic acid) nanoparticles.

Arch Biochem Biophys 2019 08 5;671:143-151. Epub 2019 Jul 5.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, 200-701, Republic of Korea. Electronic address:

Aptamer based drug delivery systems are gaining the importance in anticancer therapy due to their targeted drug delivery efficiency without harming the normal cells. The present work formulated the pH-dependent aptamer functionalized polymer-based drug delivery system against human lung cancer. The prepared aptamer functionalized doxorubicin (DOX) loaded poly (D, L-lactic-co-glycolic acid) (PLGA), poly (N-vinylpyrrolidone) (PVP) nanoparticles (APT-DOX-PLGA-PVP NPs) were spherical in shape with an average size of 87.168 nm. The crystallography and presence of the PLGA (poly (D, L-lactic-co-glycolic acid)) and DOX (doxorubicin) in APT-DOX-PLGA-PVP NPs were indicated by the X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FTIR), and H and C nuclear magnetic resonance spectrometer (NMR). The pH-dependent aptamer AS1411 based drug release triggered the cancer cell death was evidenced by cytotoxicity assay, flow cytometry, and fluorescent microscopic imaging. In addition, the cellular uptake of the DOX was determined and the apoptosis-related signaling pathway in the A549 cells was studied by Western blot analysis. Further, the in vivo study revealed that mice treated with APT-DOX-PLGA-PVP NPs were significantly recovered from cancer as evident by mice weight and tumor size followed by the histopathological study. It was reported that the APT-DOX-PLGA-PVP NPs induced the apoptosis through the activation of the apoptosis-related proteins. Hence, the present study revealed that the APT-DOX-PLGA-PVP NPs improved the therapeutic efficiency through the nucleolin receptor endocytosis targeted drug release.
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http://dx.doi.org/10.1016/j.abb.2019.07.004DOI Listing
August 2019

Effect of Rice Processing towards Lower Rapidly Available Glucose (RAG) Favors , a South Indian Fermented Food Suitable for Diabetic Patients.

Nutrients 2019 Jun 30;11(7). Epub 2019 Jun 30.

Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, Korea.

The Asian food pattern primarily embraces rice and rice-based products, which mainly comprise 90% starch. Among these various food products, is mostly prepared through fermentation. It has high palatability, and the rapid and highly digestible nature of the food product results in a higher post-glucose level in diabetic patients. A heat-treated rice sample was prepared by roasting parboiled rice at the temperature range of 155 to 165 °C for 65 to 75 s. /rice-based Dokala made from heat-treated rice is better when compared to untreated rice in terms of its microbiological profile and physiochemical properties. The proximate composition of heat-treated parboiled rice /Rice Dokala showed slightly higher values than the untreated parboiled rice /Rice Dokala, which reveals that it has marginally higher nutritive value. Determination of the Rapidly Available Glucose (RAG) and Slowly Available Glucose (SAG) values, SEM analysis, resistant starch analysis, microbial assay, and in vivo studies were performed to determine the glycemic index (GI) and glycemic load in normal and diabetic persons. Sensory analysis also proved that heat-treated /Rice Dokala is superior to untreated based on the color, flavor, appearance, taste, and texture.
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http://dx.doi.org/10.3390/nu11071497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6683079PMC
June 2019

Mycosynthesis, characterization, anticancer and antibacterial activity of silver nanoparticles from endophytic fungus .

Int J Nanomedicine 2019 9;14:3427-3438. Epub 2019 May 9.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea.

Biogenic silver nanoparticles (AgNPs) have wider range of biomedical applications. The present work synthesized Tp-AgNPs using mycelial extract of endophytic fungus (MEEF), characterized, and analyzed for antibacterial, anti-proliferation and cell wounding healing activities. The synthesized Tp-AgNPs were characterized by UV-visible spectrophotometer (UV-Vis), field emission transmission electron microscopy (FETEM) with energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), particle size analysis (PSA) and X-ray diffraction (XRD). Further, antibacterial activity was determined by Kirby-Bauer test and anti-proliferation activity was tested in human lung carcinoma A549 by water-soluble tetrazolium and flow cytometer assay. In addition, cell wounding healing activity was determined by scratch assay. UV-Vis results displayed a strong absorption peak from 390 nm to 420 nm, which indicated the successful synthesis of Tp-AgNPs. FETEM-EDS results indicated the round and triangle shaped Tp-AgNPs with the average size of 25 nm in accordance with PSA. FTIR analysis indicated the involvement of various functional molecules from MEEF in the synthesis of Tp-AgNPs. XRD result proved nature of Tp-AgNPs as a high-quality crystal. The Tp-AgNPs significantly inhibited the growth of bacterial pathogens at the minimal inhibitory concentration of 16.12 μg.mL for Gram, and 13.98 μg.mL for Gram bacteria. Further, Tp-AgNPs (2 μg.mL) showed a strong anti-proliferation effect in A549. Interestingly, Tp-AgNPs was not cytotoxic to normal NIH3T3 cells. In addition, the NPs exhibited a strong cell wounding healing activity. This work biosynthesized AgNPs with strong antibacterial, anticancer and cell wound healing properties using endophytic fungus .
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http://dx.doi.org/10.2147/IJN.S200817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6515543PMC
August 2019

Unveiling the potentials of biocompatible silver nanoparticles on human lung carcinoma A549 cells and Helicobacter pylori.

Sci Rep 2019 04 8;9(1):5787. Epub 2019 Apr 8.

Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon, 24341, Republic of Korea.

Silver nanoparticles (AgNPs) are gaining importance in health and environment. This study synthesized AgNPs using the bark extract of a plant, Toxicodendron vernicifluum (Tv) as confirmed by a absorption peak at 420 nm corresponding to the Plasmon resonance of AgNPs. The AgNPs were spherical, oval-shaped with size range of 2-40 nm as evident by field emission transmission electron microscopy (FE-TEM) and particle size analysis (PSA). The particles formed were crystalline by the presence of (111), (220) and (200) planes, as revealed by X ray diffraction (XRD) and energy dispersive spectroscopy (EDS). The presence of amine, amide, phenolic, and alcoholic aromatics derived from Tv extract was found to be capping and or reducing agents as evident by Fourier-transform infrared spectroscopy (FTIR) spectra. The Tv-AgNPs were observed to be biocompatible to chick embryonic and NIH3T3 cells at various concentrations. Interestingly, Tv-AgNPs at the concentration of 320 µg. mL induced 82.5% of cell death in human lung cancer, A549 cells and further 95% of cell death with annexin V FITC/PI based apoptosis. The Tv-AgNPs selectively targeted and damaged the cancer cells through ROS generation. The Tv-AgNPs displayed minimal inhibitory concentration (MIC) of 8.12 µg.mL and 18.14 µg.mL against STEC and H. pylori respectively. This multi-potent property of Tv-AgNPs was due to shape and size specific property that facilitated easy penetration into the bacterial and cancer cells for targeted therapy.
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http://dx.doi.org/10.1038/s41598-019-42112-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453883PMC
April 2019