I am Dr. Haider, a Post-doc researcher at Nanjing Agricultural University, China. My major expertise are:
>Plant molecular biology
>High-throughput sequencing tech
Primary Affiliation: Nanjing Agricultural University - Nanjing, Jiangsu , China
Date palm (Phoenix dactylifera L.) is an important part of human diet due to the impressive panel of sugars, polyphenols, antioxidants and essential elements. In this study, ten elite indigenous Pakistani date palm cultivars were characterized for their fruit morphological and biochemical traits at three fruit ripening stages. Results depicted significant distinction in size, shape and fruit dimensions during their different softening patterns. Moreover, the moisture contents, soluble protein contents, total phenolic contents (TPC), antioxidant activity (AA), specific activity of catalase (CAT) and peroxidase (POD) were high at khalal stage, followed by rutab stage, and lowest at tamar stage. On the converse, pH and reducing sugars (glucose and fructose) increased up to the full-ripe stage in all the investigated cultivars. Pearson’s test was also established in fruit morphological parameters and sugar components. The disparity in nutritional composition mainly depended on the type of cultivar and fruit maturation stages. Our findings revealed that the indigenous date palm germplasm was the potential source of sugar contents and variety of antioxidants and could possibly be used as functional food components.
Nafees, M., M.J. Jaskani, S.A. Naqvi, M.S. Haider and I.A. Khan, 2018. Evaluation of elite pomegranate genotypes of Balochistan based on morphological, biochemical and molecular traits. Int. J. Agric. Biol., 20: 1405‒1412
Jia, H. , Wang, C. , Zhang, C. , Haider, M. , Zhao, P. , & Liu, Z. , et al. (2016). Functional analysis of vvbg1 during fruit development and ripening of grape. Journal of Plant Growth Regulation, 1-13.
Journal of Plant Growth Regulation
b-glucosidase (BG) was believed to take part in abscisic acid (ABA) synthesis via hydrolysis of ABA glucose ester to release active ABA during plant growth and development. However, there is no genetic evidence available to indicate the role of genes during fruit ripening. Here, the expression patterns of three genes (VvBG1, VvBG2, and VvBG3) encoding b-glucosidase were analyzed during grape fruit development, and it was found that b-glucosidase activity increased in grape fruit in response to various stresses. Furthermore, to verify the function of b-glucosidase during fruit ripening, heterogeneous expression of the VvBG1 gene in strawberry fruit was validated, and the results showed that the VvBG1 overexpression increased b-glucosidase and promoted the fruit ripening process in strawberry. In addition, we found that
ABA contents increased in the VvBG1 over-expression of strawberry fruit, which induced fruit anthocyanin, soluble
solid accumulation, and fruit softening. Moreover, genes related to coloring (CHS, CHI, F3H, and UFGT), softening
(PG1, PL1, and EXP1), and aroma (SAAT, and QR) were up-regulated. This work will elucidate the specific roles of
VvBGs in the synthesis of ABA and provide some new insights into the ABA-controlled grape ripening mechanism.
Date palm is a dioecious monocotyledonous plant which belongs to Arecaceae family. Date palm pollen retains
viability for a single growing season. Air dried pollen was enclosed in sealed capsules and stored at 4°C, -20°C and -80°C
for different storage periods (1, 3, 6, 12 months). Pollen viability of fresh and stored pollen was determined by in vitro
germination and staining test. After thawing, stored pollens were cultured in petri dishes and incubated at three different
temperature i.e., 20°C, 25°C and 30°C for different time periods (3, 6, 12 and 24 hours). Pollen grains looking normal and
stained red were considered viable, whereas weakly stained or colorless were recorded as non viable. Germination test
showed that pollen of cultivar Khadrawy stored at -20°C had higher germination (71.22%) when incubated at 30°C for 24
hours after 12 months of storage while least was in Hillawi i.e. 34.86%.
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