Publications by authors named "Muhammad Naveed Aslam"

4 Publications

  • Page 1 of 1

Exogenous melatonin mitigates chromium toxicity in maize seedlings by modulating antioxidant system and suppresses chromium uptake and oxidative stress.

Environ Geochem Health 2021 Apr 2. Epub 2021 Apr 2.

Department of Forestry and Wildlife, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.

Melatonin, being an endogenous signaling molecule plays important role in plant growth and stress alleviation. The present study was conducted to evaluate the ameliorative role of melatonin against Cr toxicity in maize seedlings. The Cr toxicity (50, 100 and 200 µM) severely affected hydroponically grown seedlings growth in a dose-dependent manner; however, the melatonin (0.5 and 1.0 µM) application markedly restored toxicity-induced growth retardation. Higher dose of melatonin (1.0 µM) was more effective in case of lower Cr toxicity (50 and 100 µM). Exposure of 200 µM Cr caused 45% and 43% reduction in shoot and root lengths and more than 80% reduction in biomass. In case of 200 µM Cr toxicity, application of 1.0 µM MT effectively restored shoot and root lengths reduction (from 45 to 30%) and biomass decline (from 80 to around 60%). Biomass restoration by 1.0 µM melatonin under 50 and 100 µM Cr was even more pronounced bringing it near to control plants having no Cr exposure. Further, both melatonin levels also improved root tips, root diameter, root volume and root surface area that had been damaged by Cr exposure. The melatonin also alleviated Cr-induced chlorophyll and carotenoids inhibition, improved relative water content, and markedly lowered proline and MDA content in shoots. Lower accumulation of MDA and proline, and greater membrane stability indices indicate that the melatonin conferred better plant growth by playing the role of antioxidant and detoxifying oxidative stress creating substances. Although antioxidant enzymes viz. SOD, POD, CAT and APX activities were also elevated by MT, this increase was not significantly different in the most of cases. No significant difference in NPK contents of shoot was observed by Cr and melatonin application indicating the growth retardation being caused directly by Cr intrinsic toxicity and not by nutrients deficiency. The melatonin-based amelioration of Cr toxicity in maize seedlings seems as the result of its nature as antioxidant, and not by activation/elevation of antioxidative enzymatic system.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10653-021-00908-zDOI Listing
April 2021

Evaluation of Antibiotic Resistance and Virulence Genes among Clinical Isolates of Pseudomonas aeruginosa from Cancer Patients.

Asian Pac J Cancer Prev 2020 May 1;21(5):1333-1338. Epub 2020 May 1.

Department of Microbiology, University of Central Punjab Lahore, Pakistan.

Objectives: The objectives of this study were to evaluate P. Aeruginosa isolates from cancer patients for the phenotypic pattern of antibiotic resistance and to detect the gene responsible for virulence as well as antibiotic resistance.

Methods: A total of 227 P. aeruginosa isolates were studied and 11 antibiotics were applied for susceptibility testing. PCR detection of the genes BIC, TEM, IMP, SPM, AIM, KPC, NDM, GIM, VIM, OXA, toxA and oprI was done. Finally, the carbapenem resistant isolates were tested for phenotypic identification of carbapenemase enzyme by Modified Hodge test.

Results: The results showed that the isolates were resistant to imipenem (95%), cefipime (93%), meropenem (90%), polymixin B (71%), gentamicin (65%), ciprofloxacin (48%), ceftazidime (40%), levofloxacin (39%), amikacin (32%), tobramycin (28%) and tazobactum (24%). The PCR detection of the carbapenem resistant genes showed 51% isolates were positive for IMP, GIM and VIM, 38% for AIM and SPM, 30% for BIC, 20% for TEM and NDM, 17% for KPC and 15% for OXA. However, toxA and oprI genes were not detected. 154 carbapenem resistant isolates were found positive phenotypically for carbapenemase enzyme identification by Modified Hodge test.

Conclusion: The co-existence of multiple drug-resistant bodies and virulent genes has important implications for the treatment of patients. This study provides information about treating drug-resistant P. Aeruginosa and the relationship of virulent genes with phenotypic resistance patterns.
.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.31557/APJCP.2020.21.5.1333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541853PMC
May 2020

First record and taxonomic description of the genus (Fabricius) (Lepidoptera: Noctuidae: Plusiinae) from Pakistan.

Saudi J Biol Sci 2020 May 13;27(5):1375-1379. Epub 2019 Dec 13.

Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia.

Species belonging to genus, Fabricius of the subfamily Plusiinae which are polyphagous in nature and pest of vegetables, foods, legumes, fodder, fruits, ornamental plants and cotton crops. Samples were collected from different localities of district Bahawalpur. For collection, comprehensive and comparative surveys were carried out during 2017-18 on taxonomic account of species of the genus Fabricius and resulted identified one species (Fabricius) first time from Pakistan. Morphological characters viz., vertex, frons, labial palpi, antennae, compound eyes, ocelli, proboscis, wing venation, male and female genital characteristics were used for the identification and classification. Dichotomous keys and photographs are also provided. There is hardly any substantial research work on taxonomic studies of subfamily Plusiinae Pakistan. So to fill this gap the present proposal was designed to study the diversity of Noctuid moths from Pakistan and very fruitful results have been obtained.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sjbs.2019.12.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182779PMC
May 2020

Assessing indigenous and local knowledge of farmers about pollination services in cucurbit agro-ecosystem of Punjab, Pakistan.

Saudi J Biol Sci 2020 Jan 3;27(1):189-194. Epub 2019 Jul 3.

Research Center for Advanced Materials Science (RCAMS), King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia.

Being the ultimate beneficiary of ecosystem services provided by on-farm agricultural biodiversity, the participation of farmers in its sustainable utilization and conservation is crucial. How much aware they are with the significance and conservation of agricultural biodiversity in order to improve their crop yield remains unclear, especially from the developing courtiers. Pollination is one of such ecosystem services, enormously contributed by the wild bees. In the present study, we have investigated the knowledge of farmers about bees and pollination in general in three districts i.e. Multan, Bahawalpur and Khanewal of southern Punjab, Pakistan. Some 300 farmers (100 cucurbit growers in each district using convenient sampling method) were interviewed using a semi-structured questionnaire. Respondents were first presented with a box of insect specimens and then were asked to identify bees among those. Those who identified correctly were asked to state about their nesting sites. Only 11% of the respondents could correctly identify the bees and half of them could report something about nesting sites. A majority (63%) of the farmers was unable to tell fertilization requirements in cucurbits, 59% could not distinguish female flower from the male flower and 64% could not state any benefit of bees. However, upon briefing about the significance of bee pollinators, 58% of the farmers showed eagerness to conserve bees at their farms. Keeping in view the inadequacies of farmers' knowledge about wild bees and pollination in general, the present study also gives some policy recommendations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.sjbs.2019.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933282PMC
January 2020