Publications by authors named "Muhammad Ishaq Ali Shah"

10 Publications

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Growth performance, haematological assessment and chemical composition of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) fed different levels of Aloe vera extract as feed additives in a closed aquaculture system.

Saudi J Biol Sci 2022 Jan 6;29(1):296-303. Epub 2021 Sep 6.

Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore Pakistan.

A 105-day experimental trial was conducted to assess different levels of dietary Aleo vera extract supplementation on water quality parameters, proximate composition, growth performance and haematological parameters of fry Oreochromis niloticus. Four different percentages of dietary leaf extract powder of Aleo vera (ALE) with a basal feed, designated as, i.e., T0 (Control group; without ALE), T1 (1% ALE), T2 (2% ALE), and T3 (3% ALE). Fish fry was reared in concrete tanks (7.0 m, 1.6 m, 1.0: L, W, H; water volume 11.2 m3/tank), with an average initial weight 4.04 ± 0.03 g/ fry, and each treatment was triplicated. Fry was randomly distributed at a stocking rate of 450 individuals/ tanks. The water quality parameters revealed that temperature, pH, salinity, dissolved oxygen (DO) and nitrates were found in a promising range as given by FAO/WHO limits. However, the record values obtained for Electric Conductivity (EC), Total dissolved solids (TDS), and alkalinities were not found in all tanks' suitable range according to FAO/WHO limits. The results revealed a significant impact of different percentages of dietary ALE supplementation on fry's body composition and haematological parameters. Moreover, the final body weight, final body length, average daily weight gain (g), net weight gain (g) and specific growth rate (%) were significantly higher (p < 0.05) in T1 and T2 compared with T0 and T3 treatments. The poorest feed conversion ratio was recorded in the T2 group compared with other treatments. Thus, the current study provides information about the nutritional quality of Nile tilapia culturing in Pakistan.
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http://dx.doi.org/10.1016/j.sjbs.2021.08.098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716900PMC
January 2022

Selected Thiadiazine-Thione Derivatives Attenuate Neuroinflammation in Chronic Constriction Injury Induced Neuropathy.

Front Mol Neurosci 2021 16;14:728128. Epub 2021 Dec 16.

Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.

Neuropathic pain refers to a lesion or disease of peripheral and/or central somatosensory neurons and is an important body response to actual or potential nerve damage. We investigated the therapeutic potential of two thiadiazine-thione [TDT] derivatives, 2-(5-propyl-6-thioxo-1, 3, 5-thiadiazinan-3-yl) acetic acid [TDT1] and 2-(5-propyl-2-thioxo-1, 3, 5-thiadiazinan-3-yl) acetic acid [TDT2] against CCI (chronic constriction injury)-induced neuroinflammation and neuropathic pain. Mice were used for assessment of acute toxicity of TDT derivatives and no major toxic/bizarre responses were observed. Anti-inflammatory activity was assessed using the carrageenan test, and both TDT1 and TDT2 significantly reduced carrageenan-induced inflammation. We also used rats for the induction of CCI and performed allodynia and hyperalgesia-related behavioral tests followed by biochemical and morphological analysis using RT-qPCR, immunoblotting, immunohistochemistry and immunofluorescence. Our findings revealed that CCI induced clear-cut allodynia and hyperalgesia which was reversed by TDT1 and TDT2. To determine the function of TDT1 and TDT2 in glia-mediated neuroinflammation, Iba1 mRNA and protein levels were measured in spinal cord tissue sections from various experimental groups. Interestingly, TDT1 and TDT2 substantially reduced the mRNA expression and protein level of Iba1, implying that TDT1 and TDT2 may mitigate CCI-induced astrogliosis. molecular docking studies predicted that both compounds had an effective binding affinity for TNF-α and COX-2. The compounds interactions with the proteins were dominated by both hydrogen bonding and van der Waals interactions. Overall, these results suggest that TDT1 and TDT2 exert their neuroprotective and analgesic potentials by ameliorating CCI-induced allodynia, hyperalgesia, neuroinflammation and neuronal degeneration in a dose-dependent manner.
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http://dx.doi.org/10.3389/fnmol.2021.728128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716630PMC
December 2021

Effect of feeding frequency as a rearing system on biological performance, survival, body chemical composition and economic efficiency of Asian seabass (Bloch, 1790) reared under controlled environmental conditions.

Saudi J Biol Sci 2021 Dec 19;28(12):7360-7366. Epub 2021 Aug 19.

Department of Fisheries, Faculty of Agriculture, University of Rajshahi, Rajshahi, Bangladesh.

A 70-day rearing trial was done to determine the optimal frequency of feeding on growth performance (GP), feed conversion rate (FCR), cannibalism, survival rate (SR), body chemical composition and economic efficiency of the Asian sea bass. This study tested four different treatments of feeding frequencies (FF), once (T1), twice (T2), three times (T3), and four times (T4) per day. An average initial weight of Asian sea bass fry was 0.2 g (SD = ±0.12) were stocked 10 individuals per m (9.14 m × 1.82 m × 1.22 m, L × W × H; water depth 0.61 m) with two replicates per treatment (4 × 2 = 8). Fry were fed a mixture of larval commercial feed and shrimp with a pellet diet containing (46% CP). Initially, the feeding rate of 8% biomass per day was further adjusted according to fish biomass on a weekly basis. Results showed that, the FF significantly affected ( < 0.05) on growth indictors and survival rate (SR). Specifically fry fed three times a day (T3) had the best FBW, FL, SGR, ADWG and FCR followed by T4 and T2 while fry fed one time a day was the lowest in these parameters. Also, VSI, HSI and CF (k) significantly differed among the treatments. The fish whole body content of protein, moisture and ash did not significantly ( < 0.05) be affected by feeding frequency, but lipid content differed and both T3, T4 were the highest. It could be concluded that, increasing FF up to three times a day had a positive effect on weight gain, survival rate and feed utilization of . The second degree polynomial regression indicates that fed three times a day is optimum for best growth performance and survival for Asian sea bass.
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http://dx.doi.org/10.1016/j.sjbs.2021.08.031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8626325PMC
December 2021

Evaluating groundwater nitrate and other physicochemical parameters of the arid and semi-arid district of DI Khan by multivariate statistical analysis.

Environ Technol 2021 Oct 16:1-10. Epub 2021 Oct 16.

Government Post Graduate College for Boys Lund Khwar, Mardan, Pakistan.

Nitrate as an important water pollutant, causing eutrophication was analyzed in Pakistan at different water sources (hand pump (HP), bore hole (BH) and tube well (TW)) to assess the contamination level caused by NO. NO concentrations in the HP water samples were 31 mg L to 59 mg L, in BH 20 mg L to 79 mg L while in TW water samples it was between 29 to 55 mg L. The association of NO with other selected parameter in groundwater can be determined by using statistical approaches. Different physicochemical parameters (pH, electrical conductivity (EC), temperature and dissolved oxygen (DO)) were studied in groundwater samples of the research district. The Pearson correlation coefficient (r) for groundwater characteristics were calculated. Hierarchical Cluster Analysis (HCA) was used to categorize samples based on their groundwater quality similarities and to find links between groundwater quality factors. The key relationship of the groundwater for HP samples on EC and TDS ( = 1) had a great correlation, while all other parameters correlations were lower ( = 0.40), BH's parameters on WT and WSD ( = 0.57), WT and pH ( = 0.57), EC and DO ( = 0.50), DO and TDS (0.50), EC and TDS ( = 1) had a quite high correlation, while all other parameters correlations were less than ( = 0.40), on the other hand, tube well parameters on TDS and EC ( = 1) had a perfect correlation, DO and pH ( = 0.75) parameters correlations were less than ( = 0.40).
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http://dx.doi.org/10.1080/09593330.2021.1987532DOI Listing
October 2021

Assessing the physico-chemical parameters and some metals of underground water and associated soil in the arid and semiarid regions of Tank District, Khyber Pakhtunkhwa, Pakistan.

Environ Monit Assess 2021 Aug 30;193(9):610. Epub 2021 Aug 30.

Department of Chemistry, Abdul Wali Khan University Mardan, Mardan, 23200, KPK, Pakistan.

Good-quality water and food are the basic needs of humans, plants, and animals. Polluted groundwater and soil directly and indirectly affect organisms, which is the main environmental concern. In the current study, standard protocols of atomic absorption spectrometry were adopted for the investigation of selected metals (lead, chromium, and iron) in the collected groundwater and soil samples. The Pearson correlation coefficient (r) applied to groundwater and soil samples shows a positive perfect correlation among water parameters (conductivity and total dissolved solids) in all three sources. In the hand pump samples between water table (WT) and water source depth (WSD), Pearson correlation coefficient (r) value was found (r = 0.87) while between EC and TDS, it was r = 1. Similarly, in the bore hole samples between WT and WSD (r = 0.66), EC and TDS (r = 1), EC and Cr (r = 0.70), and TDS and Cr (r = 0.70), which showed weaker correlation. In the tube well samples, correlation between EC and TDS was high (r = 1). The correlation coefficient (r) values of the soil parameters in the hand pump (soil) samples between Fe and Cr (r = 0.86), in bore hole samples between Fe and Cr (r = 0.77), in tube well samples between Fe and Cr (r = 0.69), while all the other parameter correlations were found lower (r = 0.60). Between electrical conductivity and total dissolved solids, high relation has been observed between them (r = 1). Overall, results showed that in most of the studied samples, contents of the target metals were found above the allowable limit set by the World Health Organization (WHO) and the United States Environmental Protection Agency (USEPA).
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http://dx.doi.org/10.1007/s10661-021-09370-xDOI Listing
August 2021

Zoonotic and non-zoonotic helminths in black rats of rain-fed and irrigated areas of Swat, Khyber Pakhtunkhwa, Pakistan.

Saudi J Biol Sci 2021 Apr 28;28(4):2285-2290. Epub 2021 Jan 28.

Department of Chemistry, Abdul Wali Khan University, Mardan, Pakistan.

Present study was conducted to get information on helminth parasites of zoonotic importance among the black rats of district Swat, Pakistan. Two hundred and sixty nine rats were captured from agricultural ecosystem of the district using live captured traps from 2011 to 2013. Captured rats were anesthetized and surveyed for the presence of ectoparasites, then were carefully dissected for investigation of endoparsites. Helminth parasites of 8 species were identified. Presence of parasite was noticed in 23.7% of sampled rats. The infection rates of sampled rats was given in order of their infectivity as 13(4.83%), 13(4.83%), 12 (4.46%), . 9(3.34%), 8(2.97%), 4(1.48%), 4(1.48%) and spp. 1 (0.37%). No significant difference (P < 0.4289) was found in prevalence of parasites among areas, crops, crop stages and sex of the host while adult rats were found more infected than sub-adults. and were the most common species of helminths while sp., 1 (0.37%) was found only in one host. (the black rat) was regarded as the host of helminth parasites of zoonotic importance, therefore the hidden health hazards of this rodent species needed to be considered to prevent infectivity of humans. Current study was concluded that harbored a wide variety of helminth parasites which shows a hidden risk to inhabitants of the region. Monitoring rats' population in settle areas and educating the local community about the risk of rat borne parasitic diseases transmission through rats appears to be absolutely essential.
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http://dx.doi.org/10.1016/j.sjbs.2021.01.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071885PMC
April 2021

Acute oral toxicity and antioxidant studies of an amine-based diselenide.

BMC Complement Altern Med 2019 Apr 3;19(1):80. Epub 2019 Apr 3.

Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, CEP 97105-900, Brazil.

Background: Organochalcogen compounds have attracted the interest of a multitude of studies for their promising Pharmacological and biological activities. The antioxidant activity and acute toxicity of an organoselenium compound, 1-(2-(2-(2-(1-aminoethyl)phenyl)diselanyl)phenyl)ethanamine (APDP) was determined in mice.

Methods: Mice were randomly divided into four groups, with each group comprising of seven animals. Canola oil (1ml/kg of body weight) was administered to 1st group, while 2nd, 3rd & 4th groups were administered with 10 mg/kg, 30 mg/kg & 350 mg/kg of APDP respectively. APDP was administered by Intragastric gavage as a single oral dose.

Results: The APDP oral administration was found to be safe up to 350 mg/kg of body weight and no deaths of animals were recorded. The lethal dose 50 (LD) for APDP was determined at 72 h and was estimated to be > 350 mg/kg. After acute treatment, all mice were sacrificed by decapitation to determine the antioxidant enzymes and lipid peroxidation values for the treated mice liver. No fluctuation in lipid peroxidation, vitamin C and non protein thiol (NPSH) levels was observed due to the administration of APDP. hepatic α-ALA-D activity, catalase (CAT), superoxide dismutase (SOD) and the biochemical parameters were evaluated. Experimental observation demonstrated that APDP protected Fe(II) induced thiobarbituric acid reactive substances (TBARS) production in liver homogenate significantly (p < 0.05). The administration of APDP (an amine-based diselenide) both in vitro and in vivo clearly demonstrated that this potential compound has no acute toxicity towards mice among all the tested parameter.

Conclusion: On the basis of experimental results, it is concluded that APDP is a potential candidate as an antioxidant compound for studying pharmacological properties.
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http://dx.doi.org/10.1186/s12906-019-2489-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448241PMC
April 2019

Pharmacological mechanisms underlying gastroprotective activities of binapthyl diselenide in Wistar rats.

Inflammopharmacology 2018 Aug 13;26(4):1117-1123. Epub 2018 Mar 13.

Programa de Pós-Graduação em Ciências Biológicas- Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, CEP 97105-900, Brazil.

Selenium (Se) is a dietary essential trace element with important biological roles. It is a nutrient related to the complex metabolic and enzymatic functions. Organoselenium compounds have been reported to have anti-ulcer activity and used as drug for the treatment of gastrointestinal disorders. The antiulcer activity of binapthyl diselenide (NapSe)2 was investigated in ethanol-induced gastric lesions in rats. A number of markers of oxidative stress were examined in rats stomach including thiobarbituric acid reactive species (TBARS), catalase (CAT), superoxide dismutase (SOD), non-protein thiol groups (NPSH) and ascorbic acid. (NapSe)2 was found to be significantly restoring the deficits in the antioxidant defense mechanisms (CAT, SOD, NPSH and ascorbic acid), and suppressed lipid peroxidation in rat stomach resulting from EtOH administration. It is experimentally concluded that ethanol exposure causes alterations in the antioxidant defense system and induces oxidative stress in rat stomach. These studies establish a promising foundation for investigating and understanding the beneficial effects of organoselenium compounds on human health. Moreover, (NaPSe) deserves further investigation as a therapeutic and preventive agent against gastric ulcer in humans.
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http://dx.doi.org/10.1007/s10787-018-0451-7DOI Listing
August 2018

2-[6-Thioxo-5-(2,4,6-trimethyl-phen-yl)-1,3,5-thia-diazinan-3-yl]acetic acid.

Acta Crystallogr Sect E Struct Rep Online 2009 Mar 28;65(Pt 4):o902. Epub 2009 Mar 28.

In the mol-ecule of the title compound, C(14)H(18)N(2)O(2)S(2), the 1,3,5-thia-diazinane-2-thione ring adopts an envelope conformation with one of the N atoms at the flap position. The plane throught the five co-planar atoms of the heterocycle is oriented at a dihedral angle of 80.59 (8)° with respect to the aromatic ring. In the crystal structure, weak inter-molecular O-H⋯S inter-actions link the mol-ecules into chains along the b axis.
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http://dx.doi.org/10.1107/S1600536809011027DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2968990PMC
March 2009

3-Benzyl-5-butyl-1,3,5-thia-diazinane-2-thione.

Acta Crystallogr Sect E Struct Rep Online 2009 Feb 6;65(Pt 3):o468. Epub 2009 Feb 6.

In the title compound, C(14)H(20)N(2)S(2), the 1,3,5-thia-diazinane-2-thione ring adopts an envelope conformation. The S=C bond length is 1.6776 (15) Å, whereas the S-C bond lengths are 1.7470 (15) and 1.8479 (17) Å. The intramolecular C-H⋯S hydrogen bond between the thione and the benzyl units along with the C-H⋯π interaction between the butyl group and the centroid of the benzene ring may be effective in stabilizing the molecule.
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http://dx.doi.org/10.1107/S1600536809003882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2968617PMC
February 2009
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