Publications by authors named "Muhammad Atiq"

13 Publications

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First Report of Lasiodiplodia pseudotheobromae Causing Stem End Rot of Mango Fruit in Pakistan.

Plant Dis 2021 Mar 3. Epub 2021 Mar 3.

Plant Pathology, University of Agriculture, Faisalabad, Punjab, Pakistan;

Mango (Mangifera indica L.) is considered a desirable fruit in international markets and is grown throughout tropical and sub-tropical countries around the world (Alemu, 2014). Stem end rot is the most damaging and complex postharvest disease of mango, resulting in losses of up to 40% in Pakistan, which is the leading producer and exporter (Alam et al. 2017). A field survey was conducted in June of 2017 and 2018 in the Rahim Yar Khan and Multan- major mango producing regions of Punjab Province. After mature but unripe mango fruit (cv. Samar Bahisht Chaunsa) were stored at 12°C for 2 weeks to permit ripening, water-soaked, dark brown to purplish black decay began to appear around the stem end portion. The decay gradually enlarged and covered the whole fruit after 7 days. Disease incidence was estimated at 30%. Small pieces (3 to 4 mm2) from the periphery of 15 diseased fruit were surface disinfected with 1% sodium hypochlorite for 2 min, rinsed three times in sterilized distilled water, air dried, and then placed aseptically onto potato dextrose agar (PDA) medium and incubated at 25°C under a 12-h light/dark photoperiod for 7 days. Twelve single-spore isolates with similar morphology were isolated from the infected tissues. Initially the fungus produced thick, fluffy and greyish-white aerial mycelium, that later turned into dark gray colonies. Conidia were unicellular, ellipsoidal, and initially hyaline, but with age became dark brown and developed a central septum. Conidia measured 24.5 to 31.5 × 11.4 to 15.7 µm (n = 60). Conidiophores were inflated at their base with one diaphragm which reduced to conidiogenous cells. Conidiogenous cells were hyaline and cylindrical. On the basis of morphological characteristics, the fungus was tentatively identified as Lasiodiplodia sp., a member of the family Botryosphaeriaceae (Alves et al. 2008). For molecular identification, genomic DNA was extracted from mycelium following the CTAB method. The internal transcribed spacer (ITS) region of rDNA and translation elongation factor 1-alpha (TEF1-α) gene were amplified using ITS1/ITS4 (White et al. 1990) and EF1-728F/EF1-986R primer sets (Carbone and Kohn 1999), respectively. BLASTn searches of sequences revealed 99% to 100% identity with the reference sequences of various Lasiodiplodia pseudotheobromae isolates (GenBank accession nos. MH057189 for ITS; MN638768 for TEF-1a). The sequences were deposited in GenBank (accession nos. MW439318, MW433883 for ITS; and MW463346, MW463347 for TEF-1a). To fulfill Koch's postulates, a suspension of 105 conidia/ml from a 7-day-old culture of L. pseudotheobromae was used to inoculate fully mature but unripe mango fruit (cv. Samar Bahisht Chaunsa). Fruit were pricked with a sterilized needle to a depth of 4 mm at the stem end portion, injected with 50 μl of the prepared spore suspension (Awa et al. 2012), and stored at 12°C for 3 weeks under 70 to 80% RH. Twenty mango fruit were inoculated, and 10 were inoculated with sterile water only. After 15 days, most fruit showed typical symptoms at the stem end. Reisolations from symptomatic fruit following the procedures described above for isolating and identifying the fungal cultures from infected field samples, consistently yielded a fungus identical to L. pseudotheobromae. Control fruit remained disease-free. Although L. pseudotheobromae was previously reported on several forest and fruit trees (Alves et al. 2008; Awan et al. 2016), this is the first report of the pathogen causing stem end rot disease of mango in Pakistan. This report is important for the new studies aiming at management of stem end rot disease of mango caused by L. pseudotheobromae in Pakistan.
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http://dx.doi.org/10.1094/PDIS-01-21-0099-PDNDOI Listing
March 2021

A Case of Docetaxel-Induced Rhabdomyolysis.

Cureus 2020 Jul 25;12(7):e9380. Epub 2020 Jul 25.

Hematology and Oncology, Thomas Jefferson University, Philadelphia, USA.

Docetaxel is an antimicrotubule agent with activity in a variety of cancers. Its toxicity profile includes myelosuppression, fluid retention/edema, and peripheral neuropathy. It is also associated with myalgias but the frequency and extent of this toxicity are not well described. Here, we present a case of a 48-year-old female with breast cancer who developed bilateral proximal leg pain and inability to walk two weeks after the third cycle of docetaxel and cyclophosphamide. Initial workup showed elevated creatinine kinase (CK) levels which trended up to 9000 U/L suggesting rhabdomyolysis. She was treated with IV isotonic fluids without renal complications. Her CK was 1800 U/L at discharge and normalized two weeks post-discharge. To our knowledge, this is one of the few reports of docetaxel-induced rhabdomyolysis. The purpose of this report is to alert physicians of this rare but morbid complication.
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http://dx.doi.org/10.7759/cureus.9380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447151PMC
July 2020

Takotsubo cardiomyopathy: a rare complication of acute viral gastroenteritis.

J Community Hosp Intern Med Perspect 2020 Jun 14;10(3):258-261. Epub 2020 Jun 14.

Internal Medicine, Abington - Jefferson Health, Abington, PA, USA.

Takotsubo Cardiomyopathy (TCM) is characterized by a transient but reversible ventricular dysfunction in post-menopausal females following, but not always, a recent emotional or physical stress. Typically, chest pain is reported as a presenting symptom in the majority of patients. The severe diarrheal illness secondary to acute viral gastroenteritis is not commonly reported as the stressor event prior to TCM. We report a unique case of a middle-aged male presented with syncope shortly after loose bowel movements. He was diagnosed with TCM and was successfully managed with supportive care. The purpose of this case is to make clinicians aware of this rare association.
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http://dx.doi.org/10.1080/20009666.2020.1767273DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426982PMC
June 2020

Rhabdomyosarcoma from uterus to heart.

J Community Hosp Intern Med Perspect 2020 Jun 14;10(3):245-249. Epub 2020 Jun 14.

Internal Medicine, Resident Physician Jefferson Health - Abington.

Rhabdomyosarcoma (RMS) is a malignant soft tissue tumor of the pediatric population which is  rarely seen in adults. Metastatic rhabdomyosarcoma is even rarer. We present an unusual case of a 49 year old female presenting with palpitations and uterine bleeding. An Echo-cardiogram revealed a large oval mass on the posterior mitral leaflet and a Computerized Tomography (CT) scan of the abdomen revealed a uterine growth. Surgical excision of the cardiac mass was done and histological analysis of cardiac lesion confirmed it to be rhabdomyosarcoma with a primary source in the uterus. The patient became asymptomatic from a cardiac standpoint after excision of the mass and was scheduled for chemo/radiation therapy for the primary uterine malignancy. Metastatic cardiac rhabdomyosarcoma can be confused with a myxoma or any other primary or secondary cardiac tumors resulting in delayed diagnosis. However, its aggressive nature makes it a life-threatening tumor that requires an early diagnosis to prevent fatal consequences.
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http://dx.doi.org/10.1080/20009666.2020.1760465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7426992PMC
June 2020

Developmental Differences on the Internal Reproductive Systems between the Prediapause and Prereproductive Adults.

Insects 2020 Jun 4;11(6). Epub 2020 Jun 4.

Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

(Heteroptera: Alydidae), an important crop pest, is capable of entering reproductive adult diapause under short-day photoperiods. Though the physiological aspects of adult diapause have been well studied in this species, little is known about its morphological development. In the present study, the adult females are discriminated as prediapause and prereproductive based on the absence and presence of mature oocytes in ovarioles, respectively. We also measured the morphological development of vitellarium and lateral oviduct in females, and the accessory gland, ejaculatory duct, and testes in males of both prereproductive and prediapause adults. Our results revealed that there is a clear significant difference in the reproductive development of prediapause and prereproductive insects. Moreover, the internal morphology of reproductive organs was suppressed in prediapausebugs compared to prereproductive bugs, and the insects developedthe reproductive parts as newly emerged adults. The above findings provide basic knowledge on the characterization of diapause and reproductive adults, which would be applicable to molecular investigations.
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http://dx.doi.org/10.3390/insects11060347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7349029PMC
June 2020

Species Diversity of and Causing Potato Blackleg Disease in Pakistan.

Plant Dis 2020 May 8;104(5):1492-1499. Epub 2020 Mar 8.

Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.

Potato blackleg is caused by a diverse species of pectinolytic bacteria. In Pakistan, approximately 90% of the pathogens involved belong to Survey (2014 to 2017), sampling, and isolation from different potato growing areas of Punjab, Pakistan depicted an overall disease incidence of approximately 15%. Thirty-six pectinolytic strains confirmed through biochemical and pathogenicity testing were characterized via gene to identify them at the species level. To further validate the identification, one strain from each species SS26 (), SS28 (), SS70 (), SS90 (), SS95 (), and SS96 () were selected for draft genome sequencing and multilocus sequence analysis of 13 housekeeping genes (, , , , , , , , , , , , and ). Phylogenetic analysis revealed considerable genetic diversity in the genus . DNA-DNA hybridization and average nucleotide identity values of the strains selected for genome sequencing were determined with other reference and strains. Moreover, all six representative strains were also phenotypically characterized on the basis of metabolism of different carbon sources. Overall, on the basis of genotypic and phenotypic characteristics, these 36 isolates were grouped into six species: , , , , , and .
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http://dx.doi.org/10.1094/PDIS-08-19-1743-REDOI Listing
May 2020

Unexpected Outcome of Daptomycin-induced Eosinophilic Pneumonia: Rarity Within a Rarity.

Cureus 2019 Dec 2;11(12):e6271. Epub 2019 Dec 2.

Pulmonary and Critical Care Medicine, Abington Hospital-Jefferson Health, Abington, USA.

Eosinophilic pneumonia comprises a group of lung diseases in which eosinophils appear in increased numbers in the lungs and sometimes in the bloodstream. Among several causes of pulmonary eosinophilia, drug-induced pulmonary eosinophilia and subsequent pneumonia is a well-known side effect of many medications. Daptomycin is now being increasingly recognized culprit medication in patients with eosinophilic pneumonia. Here we present a patient with daptomycin-induced acute eosinophilic pneumonia, who had an unusual response to usual treatment.
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http://dx.doi.org/10.7759/cureus.6271DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6937480PMC
December 2019

Idiosyncratic Reaction Causing a Rare Side Effect: Isotretenoin-induced Pancreatitis.

Cureus 2019 Nov 8;11(11):e6102. Epub 2019 Nov 8.

Internal Medicine, Abington Hospital-Jefferson Health, Abington, USA.

Isotretinoin is a frequently prescribed medication for severe nodulocystic acne. It is also used in higher doses and other forms to treat some carcinomas. Pancreatitis remains a well-known but rare side effect of this medication. Two proposed mechanisms for pancreatitis are hypertriglyceridemia induced and idiosyncratic reaction. Here, we present a case of a young man who presented for the evaluation of abdominal pain. His blood work showed elevated lipase levels but computed tomography (CT) of the abdomen did not show any pancreatic inflammation.
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http://dx.doi.org/10.7759/cureus.6102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901366PMC
November 2019

Idiosyncratic Drug Reaction: A Rare Mechanism of Acute Tylenol Toxicity.

Cureus 2019 Nov 8;11(11):e6099. Epub 2019 Nov 8.

Internal Medicine, Abington Hospital-Jefferson Health, Abington, USA.

Acetaminophen (APAP) is perhaps the most commonly used drug both inside and outside the hospital due to its relative safety and over-the-counter availability. Despite its safety, it can cause drug-related side effects, especially acute liver injury that can be unpredictable. Additionally, due to its variable, delayed and nonspecific symptomatology, it can pose a significant diagnostic challenge. Due to potential reversibility with an antidote and adverse outcome related to liver failure, timely recognition and treatment is key in suspected toxicity. Here we present a case of a young female who presented for the evaluation of seizure and found to have APAP-related liver failure with only 2 g of APAP taken over two days duration.
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http://dx.doi.org/10.7759/cureus.6099DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901368PMC
November 2019

Outcomes after Surgical Resection of Hilar Cholangiocarcinoma.

J Coll Physicians Surg Pak 2019 Sep;29(9):874-877

Department of BPB Surgery and Liver Transplant, Shifa International Hospital, Islamabad, Pakistan.

Objective: To report long term outcomes after surgical resection of hilar cholangiocarcinoma (hCCA).

Study Design: Cohort study.

Place And Duration Of Study: Department of Liver Transplant and Hepatopancreaticobiliary Surgery, Shifa International Hospital, Islamabad, Pakistan, from October 2011 to April 2018.

Methodology: A prospective review of maintained database of patients who underwent surgical resection for hCCA was performed. A total of 24 patients were included. Outcome was assessed, based upon 90-day morbidity and mortality, 5-year recurrence-free survival (RFS) and overall survival (OS).

Results: Median age was 49 (23-73) years. Male to female ratio was 1.4:1. Median CA 19-9 level was 113 (2-1200) U/ml. Nine patients (37.5%) underwent right hepatectomy, six had right trisectionectomy (25%), three had central hepatectomy (12.5%) and left hepatectomy (12.5%) each, while three (12.5%) had other surgical procedures. In addition, two (8.3%) patients required portal vein resection and reconstruction. Median blood loss was 1350 (100-2000) ml. Median ICU stay was 4 (2-13) days, while hospital stay was 10 (6-32) days. Sixteen (66.7%) patients experienced at least one morbidity within 90 days, while 90-day mortality was 1/24 (4.1%). The overall recurrence rate was 6/18 (33.4%) and mortality was 9/18 (50%). The actuarial 5-year RFS was 60% and OS was 39%.

Conclusion: hCCA remains a technically challenging surgical problem. Outcomes comparable to international standards can be achieved in experienced centres.
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http://dx.doi.org/10.29271/jcpsp.2019.09.874DOI Listing
September 2019

The increasing carbon dioxide (CO) due to global climate change strengthens the plants and inhibits pathogenic infection.

Environ Sci Pollut Res Int 2018 09 23;25(27):26850-26851. Epub 2018 Jul 23.

National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.

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http://dx.doi.org/10.1007/s11356-018-2787-3DOI Listing
September 2018

Antifungal exploitation of fungicides against Fusarium oxysporum f. sp. capsici causing Fusarium wilt of chilli pepper in Pakistan.

Environ Sci Pollut Res Int 2018 Mar 20;25(7):6797-6801. Epub 2017 Dec 20.

Department of Plant Pathology, College of Agriculture, BZU Bahadur Sub Campus Layyah, Layyah, Pakistan.

The research was conducted to evaluate in-vitro efficacy of numerous fungicides against Fusarium oxysporum f. sp. capsici. In present research, six treatments (T) viz. Carbendazim, Benomyl, Topsin-M, Difenoconazole, Nativo, and Alliete along with control, various concentrations (C), days (D), and their interactions, i.e., (T × C), (T × D), (C × D), and (T × C × D) were exploited in a laboratory through food poison technique. Alliete expressed maximum colony growth (1.93 cm) as compared to all other fungicides with respect to control. Interaction between treatments and concentration (T × C) exhibited maximum colony growth of all treatments (Carbendazim, Benomyl, Topsin-M, Difenoconazole, Nativo, and Alliete), i.e., 0.87, 1.23, 1.73, 2.20, 2.53, and 2.93 cm at 300 ppm as compared to 500 and 700 ppm concentrations, respectively. Similar trend was also observed concerning interaction between (fungicides × days) and (tested concentrations × days). Results of the present study revealed that among tested fungicides, Carbendazim at 700 ppm expressed significant reduction in fungal growth.
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http://dx.doi.org/10.1007/s11356-017-1032-9DOI Listing
March 2018

A comparison of three induction therapies on patients with delayed graft function after kidney transplantation.

J Nephrol 2017 Apr 9;30(2):289-295. Epub 2016 Apr 9.

Section of Nephrology and Hypertension, Department of Medicine, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA, USA.

We compare the outcomes of induction therapies with either methylprednisolone (group 1, n = 58), basiliximab (group 2, n = 56) or alemtuzumab (group 3, n = 98) in primary deceased donor kidney transplants with delayed graft function (DGF). Protocol biopsies were performed. Maintenance was tacrolimus and mycophenolate with steroid (group 1 and 2) or without steroid (group 3). One-year biopsy-confirmed acute rejection (AR) rates were 27.6, 19.6 and 10.2 % in group 1, 2 and 3 (p = 0.007). AR was significantly lower in group 3 (p = 0.002) and group 2 (p = 0.03) than in group 1. One-year graft survival rates were 90, 96 and 100 % in group 1, 2 and 3 (log rank p = 0.006). Group 1 had inferior graft survival than group 2 (p = 0.03) and group 3 (p = 0.002). The patient survival rates were not different (96.6, 98.2 and 100 %, log rank p = 0.81). Multivariable analysis using methylprednisolone induction as control indicated that alemtuzumab (OR 0.31, 95 % CI 0.11-0.82; p = 0.03) and basiliximab (OR 0.60, 95 % CI 0.23-0.98; p = 0.018) were associated with lower risk of AR. Therefore, alemtuzumab or basiliximab induction decreases AR and improves graft survival than methylprednisolone alone in patients with DGF. Alemtuzumab induction might also allow patients with DGF to be maintained with contemporary steroid-withdrawal protocol.
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http://dx.doi.org/10.1007/s40620-016-0304-7DOI Listing
April 2017