Publications by authors named "Muhammad A Alsherbiny"

9 Publications

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Portulaca oleracea seeds' extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats.

BMC Complement Med Ther 2021 Apr 14;21(1):122. Epub 2021 Apr 14.

Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.

Background: Acrylamide (ACR) is a widespread industrial and food contaminant that garnered considerable attention for its carcinogenic, neurotoxic, and reproductive toxic effects. The antioxidant effects of Portulaca oleracea seeds extract (POS) and its fertility-enhancing effects were inspiring to evaluate the protective potential and pinpoint the mechanisms and molecular targets of the UPLC-MS fingerprinted POS extract on ACR-induced testicular toxicity in rats.

Methods: Male Wistar rats were divided into 6 equal groups of negative control, ACR model (10 mg/kg b.wt.), POS at doses of (200 and 400 mg/kg b.wt.) and POS-treated ACR groups. All treatments were given by oral dosing every day for 60 days.

Results: Administration of POS extract reversed the ACR-induced epididymides weight loss with improved semen quality and count, ameliorated the ACR-decreased testicular lesion scoring, testicular oxidative stress, testicular degeneration, Leydig cell apoptosis and the dysregulated PCNA and Caspase-3 expression in a dose-dependent manner. It upregulated the declined level of serum testosterone and the expression of steroidogenic genes such as CYP11A1 and 17β3-HSD with an obvious histologic improvement of the testes with re-establishment of the normal spermatogenic series, Sertoli and Leydig cells.

Conclusions: The supplementation with POS extract may provide a potential protective effect for ACR-induced testicular dysfunction which is mediated by its antioxidant, antiapoptotic and steroidogenic modulatory effects.
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http://dx.doi.org/10.1186/s12906-021-03286-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045344PMC
April 2021

Broad-spectrum pharmacological activity of Australian propolis and metabolomic-driven identification of marker metabolites of propolis samples from three continents.

Food Funct 2021 Mar 8;12(6):2498-2519. Epub 2021 Mar 8.

NICM Health Research Institute, Western Sydney University, Penrith, NSW, Australia.

Propolis is a by-product of honeybee farming known for its broad therapeutic benefits around the world and is extensively used in the health food and beverage industry. Despite Australia being one of the world's megadiverse countries with rich flora and fauna, Australian propolis samples have not been explored adequately with most in vitro and in vivo studies centred on their Brazilian and Chinese counterparts. In view of this, our study was designed to investigate the chemical composition and anti-proliferative, antibacterial, antifungal, anti-inflammatory and antioxidant properties of Australian propolis (AP-1) extract to draw a comparison with Brazilian (BP-1) and Chinese propolis (CP-1) extracts. The AP-1 extract displayed significantly greater anti-proliferative activity against the MCF7 and the MDA-MB-231 metastatic breast adenocarcinoma cell lines compared to BP-1 and CP-1 (p < 0.05). Similar trends were also observed in the antibacterial (Escherichia coli and Staphylococcus aureus), anti-inflammatory (lipopolysaccharide-induced RAW264.7 macrophages) and antioxidant assays (ABTS, DPPH and CUPRAC) with AP-1 exhibiting more potent activity than BP-1 and CP-1. The ultra-high performance liquid chromatography (UPLC) coupled with quadrupole high-resolution time of flight mass spectrometry (qTOF-MS) and chemometrics implementing unsupervised PCA and supervised OPLS-DA analyses of the propolis samples from Australia, China and Brazil revealed 67 key discriminatory metabolites belonging to seven main chemical classes including flavonoids, triterpenes, acid derivatives, stilbenes, steroid derivatives, diterpenes and miscellaneous compounds. Additionally, seven common phenolic compounds were quantified in the samples. Further mechanistic studies are necessary to elucidate the modes of action of Australian propolis for its prospective use in the food, nutraceutical and pharmaceutical industries.
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http://dx.doi.org/10.1039/d1fo00127bDOI Listing
March 2021

The Odyssey of Bioactive Compounds in Avocado () and Their Health Benefits.

Antioxidants (Basel) 2019 Sep 24;8(10). Epub 2019 Sep 24.

School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.

, commonly known as avocado, has recently gained substantial popularity and is often marketed as a "superfood" because of its unique nutritional composition, antioxidant content, and biochemical profile. However, the term "superfood" can be vague and misleading, as it is often associated with unrealistic health claims. This review draws a comprehensive summary and assessment of research performed in the last few decades to understand the nutritional and therapeutic properties of avocado and its bioactive compounds. In particular, studies reporting the major metabolites of avocado, their antioxidant as well as bioavailability and pharmacokinetic properties, are summarized and assessed. Furthermore, the potential of avocado in novel drug discovery for the prevention and treatment of cancer, microbial, inflammatory, diabetes, and cardiovascular diseases is highlighted. This review also proposes several interesting future directions for avocado research.
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http://dx.doi.org/10.3390/antiox8100426DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6826385PMC
September 2019

LC-MS/MS quantitation of phytocannabinoids and their metabolites in biological matrices.

Talanta 2019 Nov 13;204:846-867. Epub 2019 Jun 13.

Faculty of Pharmacy & Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada. Electronic address:

Marijuana (i.e., cannabis) and its derivatives are considered the most commonly used of illicit drugs. Within the last two decades, phytocannabinoids and their synthetic analogues have emerged as potential medicines for the treatment of various disorders via targeting of the endocannabinoid system. Recently, some countries have legalized (medicinal/recreational) cannabis, which now allows for more research to be conducted. Accordingly, sensitive and specific analytical assays are required to identify and quantify these compounds in different human matrices. These analytical approaches were developed using mass spectrometric detection, where LC-MS/MS specifically has become the mainstay for the quantitative analysis of tetrahydrocannabinol and other cannabinoids. This is due to its superior selectivity and sensitivity, and ability to determine free and conjugate analytes within the same analysis. This tabular review of such methods is prefaced by a short overview of the major cannabinoids and some of their physiological actions.
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http://dx.doi.org/10.1016/j.talanta.2019.06.053DOI Listing
November 2019

Medicinal Cannabis-Potential Drug Interactions.

Medicines (Basel) 2018 Dec 23;6(1). Epub 2018 Dec 23.

NICM Health Research Institute, Western Sydney University, Westmead, NSW 2145, Australia.

The endocannabinoids system (ECS) has garnered considerable interest as a potential therapeutic target in various carcinomas and cancer-related conditions alongside neurodegenerative diseases. Cannabinoids are implemented in several physiological processes such as appetite stimulation, energy balance, pain modulation and the control of chemotherapy-induced nausea and vomiting (CINV). However, pharmacokinetics and pharmacodynamics interactions could be perceived in drug combinations, so in this short review we tried to shed light on the potential drug interactions of medicinal cannabis. Hitherto, few data have been provided to the healthcare practitioners about the drug⁻drug interactions of cannabinoids with other prescription medications. In general, cannabinoids are usually well tolerated, but bidirectional effects may be expected with concomitant administered agents via affected membrane transporters (Glycoprotein p, breast cancer resistance proteins, and multidrug resistance proteins) and metabolizing enzymes (Cytochrome P450 and UDP-glucuronosyltransferases). Caution should be undertaken to closely monitor the responses of cannabis users with certain drugs to guard their safety, especially for the elderly and people with chronic diseases or kidney and liver conditions.
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http://dx.doi.org/10.3390/medicines6010003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6473892PMC
December 2018

Ameliorative and protective effects of ginger and its main constituents against natural, chemical and radiation-induced toxicities: A comprehensive review.

Food Chem Toxicol 2019 Jan 22;123:72-97. Epub 2018 Oct 22.

NICM Health Research Institute, Western Sydney University, Westmead, 2145, NSW, Australia. Electronic address:

Fatal unintentional poisoning is widespread upon human exposure to toxic agents such as pesticides, heavy metals, environmental pollutants, bacterial and fungal toxins or even some medications and cosmetic products. In this regards, the application of the natural dietary agents as antidotes has engrossed a substantial attention. One of the ancient known traditional medicines and spices with an arsenal of metabolites of several reported health benefits is ginger. This extended literature review serves to demonstrate the protective effects and mechanisms of ginger and its phytochemicals against natural, chemical and radiation-induced toxicities. Collected data obtained from the in-vivo and in-vitro experimental studies in this overview detail the designation of the protective effects to ginger's antioxidant, anti-inflammatory, and anti-apoptotic properties. Ginger's armoury of phytochemicals exerted its protective function via different mechanisms and cell signalling pathways, including Nrf2/ARE, MAPK, NF-ƙB, Wnt/β-catenin, TGF-β1/Smad3, and ERK/CREB. The outcomes of this review could encourage further clinical trials of ginger applications in radiotherapy and chemotherapy regime for cancer treatments or its implementation to counteract the chemical toxicity induced by industrial pollutants, alcohol, smoking or administered drugs.
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http://dx.doi.org/10.1016/j.fct.2018.10.048DOI Listing
January 2019

Comparative Molluscicidal and Schistosomicidal Potentiality of Two Species and Its Isolated Glycoalkaloids.

Pharmacognosy Res 2018 Jan-Mar;10(1):113-117

Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Schistosomiasis is the most noteworthy parasitic disease after malaria. Furthermore, the significant activity of the genus against worms and its intermediate host snails reinforced the study of Andr. (SS) and L. (SM) for their molluscicidal and schistosomicidal potentiality. In this study, different extracts, fractions and isolated compounds of both species are evaluated for the molluscicidal and schistosomicidal potentialities. The niclosamide was used as positive molluscicide control against snails. Different extracts, fractions, or isolated compounds were used at a concentration of 100 μg/ml and dead snails were counted in each case. On the other hand, washed and sterilized adult worms were used in three replicates, and three worm pairs were placed in each well with 2 ml test solution of 100 μg/ml concentration. Positive (praziquantel [PZQ] 0.2 ug/ml) and negative controls were concurrently used and examined daily for 3 days for viability. The mortality rate was calculated and then both LC and LC were determined in triplicates. Highest potency was indicated to total glycoalkaloid (TGA) fraction of SM followed by TGA of SS. On the other hand, TGA fractions of both species showed higher potency than other extracts and isolated compounds. Meanwhile, solasodine-free aglycone showed declined activity compared to its glycosides. Promising molluscicidal and schistosomicidal activities were displayed which are attributed to the glycoalkaloid content. Therefore, this study can efficiently contribute toward validation of the traditional use of SS and SM in schistosomiasis control.

Summary: The current study evaluated the molluscicidal and schistosomicidal activities of different extracts and fractions of two species. The glycoalkaloids content depicted a promising activity against both the snails and the adult worms. PZQ; Praziquantel, SM; , SS; , TGA; total glycoalkaloid.
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http://dx.doi.org/10.4103/pr.pr_71_17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5855367PMC
March 2018

Synthesis and Cytotoxic Activity of Biphenylurea Derivatives Containing Indolin-2-one Moieties.

Molecules 2016 Jun 10;21(6). Epub 2016 Jun 10.

Department of Applied Organic Chemistry, National Research Center, Dokki, Giza 12622, Egypt.

In our endeavor towards the development of potent anticancer agents, two different sets of biphenylurea-indolinone conjugates, 5a-s and 8a,b were synthesized. The in vitro cytotoxicity of the synthesized compounds was examined in two human cancer cell lines, namely MCF-7 breast cancer and PC-3 prostate cancer cells using the sulforhodamine B (SRB) colorimetric assay. In particular, the MCF-7 cancer cell line was more susceptible to the synthesized compounds. Compound 5o (IC50 = 1.04 ± 0.10 μM) emerged as the most active member in this study against MCF-7, with 7-fold increased activity compared to the reference drug, doxorubicin (IC50 = 7.30 ± 0.84 μM). Compounds 5l, 5q and 8b also exhibited superior cytotoxic activity against MCF-7 with IC50 values of 1.93 ± 0.17, 3.87 ± 0.31 and 4.66 ± 0.42 μM, respectively. All of the tested compounds were filtered according to the Lipinski and Veber rules and all of them passed the filters. Additionally, several ADME descriptors for the synthesized compounds 5a-s and 8a,b were predicted via a theoretical kinetic study performed using the Discovery Studio 2.5 software.
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http://dx.doi.org/10.3390/molecules21060762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274071PMC
June 2016

Synthesis, Biological Evaluation and Molecular Docking of Certain Sulfones as Potential Nonazole Antifungal Agents.

Molecules 2016 Jan 20;21(1):E114. Epub 2016 Jan 20.

Department of Applied Organic Chemistry, National Research Center, Dokki, Giza 12622, Egypt.

We reported herein the synthesis, antifungal activity, docking and in silico ADME prediction studies of four novel series of sulfones 6a-f, 8a-c, 10a-f and 12a-c. All the newly synthesized sulfones were tested against four strains of Candida (including fluconazole-resistant Candida), two strains of Aspergillus, two dermatophytic fungi (Trichophytons mentagrophyte and Microsporum canis) and Syncephalastrum sp. with fluconazole as a reference drug. In general, compounds 8a and 10b showed selective and potent anticandidal activity (MIC: 0.19-0.81 µM) relative to fluconazole (MIC = 1.00 µM). Furthermore, 10e and 12a elicited a remarkable and selective antifungal activity against Aspergillus sp. and the dermatophytic fungi (MIC: 0.16-0.79 µM) relative to fluconazole (MIC: 2-2.6 µM). Moreover, the docking results of the sulfones 6a, 8a, 10a and 10b at the active site of CYT P450 14α-sterol demethylase showed a comparable binding interaction (interaction Energy = -34.87 to -42.43 kcal/mol) with that of fluconazole (IE = -40.37 kcal/mol).
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http://dx.doi.org/10.3390/molecules21010114DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274496PMC
January 2016