Publications by authors named "Mu Qin"

71 Publications

Self-adaptive cardiac optogenetics device based on negative stretching-resistive strain sensor.

Sci Adv 2021 Nov 24;7(48):eabj4273. Epub 2021 Nov 24.

National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Shanghai Jiao Tong University, Shanghai 200240, China.

[Figure: see text].
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http://dx.doi.org/10.1126/sciadv.abj4273DOI Listing
November 2021

Radiofrequency ablation for paroxysmal atrial fibrillation in a patient with dextrocardia and interruption of the inferior vena cava: a case report.

Eur Heart J Case Rep 2021 May 19;5(5):ytab191. Epub 2021 May 19.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, No.241 West Huaihai Road, Xuhui District, Shanghai 200030, China.

Background: Dextrocardia with interruption of the inferior vena cava (I-IVC) is a very rare anatomical variant. Catheter ablation of atrial fibrillation (AF) in patients with this anatomical variant is challenging for electrophysiologists. This case report presents a safe, effective, and radiation-free approach for high-power ablation of AF via a superior transseptal approach in patients with dextrocardia and I-IVC.

Case Summary: A 57-year-old man with paroxysmal AF with dextrocardia and I-IVC with azygos continuation was referred to our hospital for radiofrequency (RF) ablation. It was evident that transseptal puncture and pulmonary vein isolation (PVI) would be impossible using an IVC approach via the femoral vein. Therefore, we decided to perform left atrium (LA) ablation via the superior vena cava approach. A phased array intracardiac echocardiography (ICE) catheter was inserted in the right femoral vein. Three-dimensional (3D) anatomical reconstruction of LA, right atrium (RA), and coronary sinus (CS) ostium were performed using ICE with azygos vein and RA imaging. Navigation-enabled electrodes were inserted into annotated CS on cardiac 3D ICE image. The left internal jugular vein was accessed using an SL1 transseptal sheath and Brockenbrough needle. Transseptal puncture was performed under ICE with an RF-assisted approach. We accomplished ablation index guided high-power pulmonary vein isolation using a bi-directional guiding sheath with visualization capabilities and a surround flow contact force-sensing catheter. No complications occurred during or after the procedure.

Discussion: With the application of multitude of newer technologies, we can accomplish safe, effective, and fluoroscopy-free RF ablation of AF using the superior approach in patients with complex anomaly.
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http://dx.doi.org/10.1093/ehjcr/ytab191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276615PMC
May 2021

Characterization of expansin genes and their transcriptional regulation by histone modifications in strawberry.

Planta 2021 Jul 3;254(2):21. Epub 2021 Jul 3.

State Key Laboratory of Plant Genetics and Germplasm Enhancement and College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

Main Conclusion: The possible candidate expansin genes, which may be important for strawberry fruit softening, have been identified in the diploid woodland strawberry Fragaria vesca and the octoploid cultivated strawberry Fragaria × ananassa and their transcriptional regulation by histone modifications has been studied. Softening process greatly affects fruit texture and shelf life. Expansins (EXPs) are a group of structural proteins participating in cell wall loosening, which break the hydrogen bonding between cellulose microfibrils and hemicelluloses. However, our knowledge on how EXP genes are regulated in fruit ripening, especially in non-climacteric fleshy fruits, is limited. Here, we have identified the EXP genes in both the octoploid cultivated strawberry (Fragaria × ananassa) and one of its diploid progenitor species, woodland strawberry (Fragaria vesca). We found that EXP proteins in F. × ananassa were structurally more divergent than the ones in F. vesca. Transcriptome data suggested that FaEXP88, FaEXP114, FveEXP11 and FveEXP33 were the four candidate EXP genes more likely involved in fruit softening, whose transcript levels dramatically increased when firmness decreased during fruit maturation. Phylogenetic analyses showed that those candidate genes were closely clustered, indicating the presence of homoeolog expression dominance in the EXP gene family in strawberry. Moreover, we have performed chromatin immunoprecipitation (ChIP) experiments to investigate the distribution of histone modifications along the promoters and genic regions of the EXP genes in F. vesca. ChIP data revealed that the transcript levels of EXP genes were highly correlated with the enrichment of H3K9/K14 acetylation and H3K27 tri-methylation. Collectively, this study identifies the key EXP genes involved in strawberry fruit softening and reveals a regulatory role of histone modifications in their transcriptional regulation, which would facilitate functional studies of the EXP genes in the ripening of non-climacteric fruits.
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http://dx.doi.org/10.1007/s00425-021-03665-6DOI Listing
July 2021

CaMKII in Regulation of Cell Death During Myocardial Reperfusion Injury.

Front Mol Biosci 2021 1;8:668129. Epub 2021 Jun 1.

Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China.

Cardiovascular disease is the leading cause of death worldwide. In spite of the mature managements of myocardial infarction (MI), post-MI reperfusion (I/R) injury results in high morbidity and mortality. Cardiomyocyte Ca overload is a major factor of I/R injury, initiating a cascade of events contributing to cardiomyocyte death and myocardial dysfunction. Ca/calmodulin-dependent protein kinase II (CaMKII) plays a critical role in cardiomyocyte death response to I/R injury, whose activation is a key feature of myocardial I/R in causing intracellular mitochondrial swelling, endoplasmic reticulum (ER) Ca leakage, abnormal myofilament contraction, and other adverse reactions. CaMKII is a multifunctional serine/threonine protein kinase, and CaMKIIδ, the dominant subtype in heart, has been widely studied in the activation, location, and related pathways of cardiomyocytes death, which has been considered as a potential targets for pharmacological inhibition. In this review, we summarize a brief overview of CaMKII with various posttranslational modifications and its properties in myocardial I/R injury. We focus on the molecular mechanism of CaMKII involved in regulation of cell death induced by myocardial I/R including necroptosis and pyroptosis of cardiomyocyte. Finally, we highlight that targeting CaMKII modifications and cell death involved pathways may provide new insights to understand the conversion of cardiomyocyte fate in the setting of myocardial I/R injury.
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http://dx.doi.org/10.3389/fmolb.2021.668129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204011PMC
June 2021

Absence of Rgs5 Influences the Spatial and Temporal Fluctuation of Cardiac Repolarization in Mice.

Front Physiol 2021 18;12:622084. Epub 2021 Mar 18.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Aims: This study investigated the contribution of the regulator of G-protein signaling 5 (Rgs5) knockout to the alteration of the action potential duration (APD) restitution and repolarizing dispersion in ventricle.

Methods And Results: The effects of Rgs5 were investigated by QT variance (QTv) and heart rate variability analysis of Rgs5 mice. Monophasic action potential analysis was investigated in isolated Rgs5 heart. Rgs5 did not promote ventricular remodeling. The 24-h QTv and QT variability index (QTVI) of the Rgs5 mice were higher than those of wild-type (WT) mice ( < 0.01). In WT mice, a positive correlation was found between QTv and the standard deviation of all NN intervals ( = 0.62; < 0.01), but not in Rgs5 mice ( = 0.01; > 0.05). The absence of Rgs5 resulted in a significant prolongation of effective refractory period and APD in isolated ventricle. In addition, compared with WT mice, the knockout of Rgs5 significantly deepened the slope of the APD recovery curve at all 10 sites of the heart ( < 0.01) and increased the spatial dispersions of S (COV-S) (WT: 0.28 ± 0.03, Rgs5: 0.53 ± 0.08, < 0.01). Compared with WT heart, Rgs5 increased the induced S1-S2 interval at all sites of heart and widened the window of vulnerability of ventricular tachyarrhythmia ( < 0.05).

Conclusion: Our findings indicate that Rgs5 is an important regulator of ventricular tachyarrhythmia in mice by prolonging ventricular repolarization and increasing spatial dispersion in ventricle.
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http://dx.doi.org/10.3389/fphys.2021.622084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8012757PMC
March 2021

Clinical Safety and Efficacy of Ablation for Atrial Fibrillation Patients With a History of Stroke.

Front Cardiovasc Med 2021 10;8:630090. Epub 2021 Mar 10.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.

To evaluate the clinical safety and efficacy of radiofrequency catheter ablation for atrial fibrillation patients with a history of stroke. A total of 116 symptomatic, drug-refractory AF patients with a history of stroke, and 1:2 matched patients without a history of stroke were enrolled. Of these, 28 cases occurred stroke within 3 months (Group 1), 88 cases with stroke history longer than 3 months (Group 2), and 232 cases without stroke (Group 3). PVI was performed in all patients, extended to ablation of linear lesions ablation. The periprocedural stroke rates and other procedure-related in-hospital complications did not differ significantly among the three groups. The maintenance rate of SR after the procedure showed no significant difference ( = 0.333), 52.7, 66.4, and 70.7% in Group 1, 2, and 3, respectively. Furthermore, the comparison between a history of stroke and those without it were also shown no significant difference ( = 0.351). Radiofrequency ablation for AF patients occurred stroke, even within 3 months is safe and effective, without higher periprocedural complication rate and recurrence rate.
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http://dx.doi.org/10.3389/fcvm.2021.630090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7987660PMC
March 2021

Research on Carbide Characteristics and Their Influence on the Properties of Welding Joints for 2.25Cr1Mo0.25V Steel.

Materials (Basel) 2021 Feb 13;14(4). Epub 2021 Feb 13.

School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

The carbide characteristics of 2.25Cr1Mo0.25V steel have an extremely important influence on the mechanical properties of welding joints. In addition, hydrogen resistance behavior is crucial for steel applied in hydrogenation reactors. The carbide morphology was observed by scanning electron microscopy (SEM) and the carbide microstructure was characterized by transmission electron microscopy (TEM). Tensile and impact tests were carried out and the influence of carbides on properties was studied. A hydrogen diffusion test was carried out, and the hydrogen brittleness resistance of welding metal and base metal was studied by tensile testing of hydrogenated samples to evaluate the influence of hydrogen on the mechanical properties. The research results show that the strength of the welding metal was slightly higher and the Charpy impact value was significantly lower compared to the base metal. The hydrogen embrittlement resistance of the welding metal was stronger than that of the base metal. The presence of more carbides and inclusions was the main cause of the decreased impact property and hydrogen brittleness resistance of the welding metal. These conclusions have certain reference value for designing and manufacturing hydrogenation reactors.
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http://dx.doi.org/10.3390/ma14040891DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7922991PMC
February 2021

Extra-pulmonary vein driver mapping and ablation for persistent atrial fibrillation in obese patients.

Europace 2021 05;23(5):701-709

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, No.241 West Huaihai Road, Xuhui District, Shanghai 200030, China.

Aims: The aim of this study was to determine whether driver ablation effectively treats persistent atrial fibrillation (AF) in obese patients.

Methods And Results: We randomly assigned 124 persistent AF obese patients to two groups, one undergoing conventional ablation (n = 62) and the other undergoing driver ablation (n = 62). Sixty-two non-obese patients with persistent AF undergoing driver ablation served as matched controls. Bipolar electrogram dispersion was analysed for driver mapping. Epicardial adipose tissue (EAT) volume was measured using cardiac computed tomography. Obese patients had a higher proportion of driver regions in the posterior wall (56.5% vs. 32.3%, P = 0.007). Driver complexity, measured as the average number and area of driver regions, was higher in the obese group than in the non-obese group (3.5 ± 1.0 vs. 2.9 ± 0.9, P < 0.001; 15.5% ± 4.2% vs. 9.8 ± 2.6%, P < 0.001, respectively). Left atrial EAT volume correlated better with the proportion of area of driver regions than did body mass index (BMI) and total EAT (BMI: r2 = 0.250, P < 0.001; total EAT: r2 = 0.379, P < 0.001; and left atrial EAT: r2 = 0.439, P < 0.001). The rate of AF termination was significantly higher in the driver ablation group than in the conventional ablation group (82.9% vs. 22.8%, P < 0.001). During the follow-up period of 16.9 ± 6.5 months, patients in the driver ablation group had significantly better AF-free survival (91.91% vs. 79.0%, log rank test, P = 0.026) and AF/atrial tachycardia-free survival (83.9% vs. 64.5%, log rank test, P = 0.011) than did patients in the conventional ablation group.

Conclusion: Obesity is associated with increased driver complexity. Driver ablation improves long-term outcomes in obese patients with persistent AF.
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http://dx.doi.org/10.1093/europace/euaa314DOI Listing
May 2021

A Study of Cardiogenic Stroke Risk in Non-valvular Atrial Fibrillation Patients.

Front Cardiovasc Med 2020 5;7:604795. Epub 2020 Nov 5.

Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.

We attempted to develop more precisely quantified risk models for predicting cardiogenic stroke risk in non-valvular atrial fibrillation (NVAF) patients. We conducted a case-control study, using data from hospitalized patients with AF who underwent transesophageal echocardiography at Shanghai Chest Hospital. A total of 233 high cardiogenic stroke risk patients with left atrial appendage thrombus (LAT) or left atrial spontaneous echo contrast (LA-SEC) and 233 controls matched for age, sex, AF type. AF history, LA diameter enlargement, larger left ventricular end diastolic diameter, lower ejection fraction, greater serum uric acid (SUA), and brain natriuretic peptide (BNP) levels showed association with high stroke risk. The multivariate logistic regression analysis revealed that AF duration, left atrial diameter (LAd), left ventricular ejection fraction (LVEF), SUA, and BNP were independent risk factors of the LAT/LA-SEC. We used LAd, LVEF, SUA, and BNP to construct a combined predictive model for high stroke risk in NVAF patients (the area under ROC curve: 0.784; sensitivity 66.1%; specificity 76.8%; 95% CI 0.744-0.825, < 0.001). Comprehensive evaluation of LAd, LVEF, SUA, and BNP may help stratify the cardiogenic stroke risk among non-valvular AF patients, guiding anticoagulation therapy.
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http://dx.doi.org/10.3389/fcvm.2020.604795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7683797PMC
November 2020

How does nitrate regulate plant senescence?

Plant Physiol Biochem 2020 Dec 28;157:60-69. Epub 2020 Aug 28.

College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China; State Key Laboratory of Crop Biology, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China. Electronic address:

Nitrogen is an essential macronutrient for plant growth and development and plays an important role in the whole life process of plants. Nitrogen is an important component of amino acids, chlorophyll, plant hormones and secondary metabolites. Nitrogen deficiency leads to early senescence in plants, which is accompanied by changes in gene expression, metabolism, growth, development, and physiological and biochemical traits, which ensures efficient nitrogen recycling and enhances the plant's tolerance to low nitrogen. Therefore, it is very important to understand the adaptation mechanisms of plants under nitrogen deficiency for the efficient utilization of nitrogen and gene regulation. With the popularization of molecular biology, bioinformatics and transgenic technology, the metabolic pathways of nitrogen-deficient plants have been verified, and important progress has been made. However, how the responses of plants to nitrogen deficiency affect the biological processes of the plants is not well understood. The current research also cannot completely explain how the metabolic pathways identified show other reactions or phenotypes through interactions or cascades after nitrogen inhibition. Nitrate is the main form of nitrogen absorption. In this review, we discuss the role of nitrate in plant senescence. Understanding how nitrate inhibition affects nitrate absorption, transport, and assimilation; chlorophyll synthesis; photosynthesis; anthocyanin synthesis; and plant hormone synthesis is key to sustainable agriculture.
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http://dx.doi.org/10.1016/j.plaphy.2020.08.041DOI Listing
December 2020

Evolution of Welding Residual Stresses within Cladding and Substrate during Electroslag Strip Cladding.

Materials (Basel) 2020 Sep 17;13(18). Epub 2020 Sep 17.

Lanzhou LS Heavy Equipment CO., LTD, Lanzhou 730300, China.

Hydrogenation reactors are important oil-refining equipment that operate in high-temperature and high-pressure hydrogen environments and are commonly composed of 2.25Cr-1Mo-0.25V steel. For a hydrogenation reactor with a plate-welding structure, the processes and effects of welding residual stress (WRS) are very complicated due to the complexity of the welding structure. These complex welding residual stress distributions affect the service life of the equipment. This study investigates the evolution of welding residual stress during weld-overlay cladding for hydrogenation reactors using the finite element method (FEM). A blind hole method is applied to verify the proposed model. Unlike the classical model, WRS distribution in a cladding/substrate system in this study was found to be divided into three regions: the cladding layer, the stress-affected layer (SAL), and the substrate in this study. The SAL is defined as region coupling affected by the stresses of the cladding layer and substrate at the same time. The evolution of residual stress in these three regions was thoroughly analyzed in three steps with respect to the plastic-strain state of the SAL. Residual stress was rapidly generated in Stage 1, reaching about -440 MPa compression stress in the SAL region at the end of this stage after 2.5 s. After cooling for 154 s, at the end of Stage 2, the WRS distribution was fundamentally shaped except for in the cladding layer. The interface between the cladding layer and substrate is the most heavily damaged region due to the severe stress gradient and drastic change in WRS during the welding process. The effects of substrate thickness and preheat temperature were evaluated. The final WRS in the cladding layer first increased with the increase in substrate thickness, and then started to decline when substrate thickness reached a large-enough value. WRS magnitudes in the substrate and SAL decreased with the increase in preheat temperature and substrate thickness. Compressive WRS in the cladding layer, on the other hand, increased with the increase in preheat temperature.
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http://dx.doi.org/10.3390/ma13184126DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7560439PMC
September 2020

Management of catheter ablation in arrhythmia patients during the coronavirus disease 2019 epidemic.

ESC Heart Fail 2020 Sep 17. Epub 2020 Sep 17.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Aims: Health care providers have reduced outpatient services and delayed elective cardiac surgeries during the coronavirus disease 2019 (COVID-19) epidemic, while a large number of patients face more cardiovascular risks compared with those of the pandemic. The present study attempted to explore the management of catheter ablation in arrhythmia patients during the pandemic.

Methods And Results: From 1 January 2020 to 24 March 2020, a total of 269 arrhythmia patients hospitalized for catheter ablation under strict risk assessments and careful infection prevention were retrospectively analysed and considered for our analysis at our centre. Preventive measured included a 14 day quarantine, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection, adoption of personal protection equipment, application of hand disinfectant, management of hospital visitors, and maintenance of environmental hygiene. Three patients who had fever following ablation and were finally excluded from SARS-CoV-2 infection, and no hospital infection occurred during the 3.1 ± 1.5 day hospitalization period. Additionally, 258/269 (95.9%) patients were free from arrhythmia after a median 133 day follow-up (Q1, 81; Q3, 143; ending 1 June 2020), and no SARS-CoV-2 infections occurred at follow-up.

Conclusions: By adopting strict risk assessments and careful infection prevention measures, catheter ablation in patients with arrhythmia can be safely and effectively performed during the COVID-19 epidemic in well-controlled areas.
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http://dx.doi.org/10.1002/ehf2.13009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7754755PMC
September 2020

Prevalence of Cardiovascular Comorbidities in Coronavirus Disease 2019, Severe Acute Respiratory Syndrome, and Middle East Respiratory Syndrome: Pooled Analysis of Published Data.

J Am Heart Assoc 2020 09 15;9(17):e016812. Epub 2020 Jul 15.

Department of Cardiology Shanghai Chest Hospital Shanghai Jiao Tong University Shanghai China.

Background Coronavirus disease 2019 (COVID-19) is spreading widely around the world. We conducted this meta-analysis to explore the prevalence of cardiovascular comorbidities in COVID-19, severe acute respiratory syndrome (SARS), and Middle East respiratory syndrome (MERS) cases. Methods and Results Relevant reports updated to April 17, 2020, were searched from PubMed, Embase, Web of Science, and the Cochrane Library with no restriction on language. A random-effects model was used in this meta-analysis to obtain pooled proportions of cardiovascular comorbidities in COVID-19, SARS, and MERS. A total of 22 studies (12 for COVID-19, 4 for SARS, and 6 for MERS) were included in this analysis, and the average age of patients with COVID-19, SARS, and MERS was 46.41±1.79, 39.16±2.25, and 52.51±4.64 years, respectively. Proportions of cardiovascular comorbidities in coronavirus diseases were as follows: COVID-19: proportion of hypertension was 17.1% (95% CI, 13.2%-20.9%), proportion of cardiac disease was 4.5% (95% CI, 3.6%-5.5%) and proportion of diabetes mellitus was 8.5% (95% CI, 5.5%-11.4%); SARS: proportion of hypertension was 4.5% (95% CI, 2.0%-7.0%), proportion of cardiac disease was 2.1% (95% CI, 0.6%-3.7%) and proportion of diabetes mellitus was 3.7% (95% CI, 1.0%-6.4%); MERS: proportion of hypertension was 30.3% (95% CI, 18.3%-42.2%), proportion of cardiac disease was 20.9% (95% CI, 10.7%-31.1%), and proportion of diabetes mellitus was 45.4% (95% CI, 27.3%-63.5%). Conclusions The prevalence of cardiovascular comorbidities varies among different coronavirus-associated diseases. With the development of time, proportions of cardiovascular comorbidities in COVID-19 need further attention.
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http://dx.doi.org/10.1161/JAHA.120.016812DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7660764PMC
September 2020

Coronavirus Disease 2019 (COVID-19) and Cardiac Injury-Reply.

JAMA Cardiol 2020 10;5(10):1199-1200

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

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http://dx.doi.org/10.1001/jamacardio.2020.2456DOI Listing
October 2020

Key Role of Left Atrial Appendage during Redo Ablation in a Case of Long-Standing Persistent Atrial Fibrillation.

Case Rep Cardiol 2020 19;2020:9691584. Epub 2020 Jun 19.

Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Extrapulmonary vein focal sources have been recognized as the source of atrial fibrillation in some cases, and empiric electric isolation of the left atrial appendage has been proposed for long-standing persistent atrial fibrillation by some. Here, we present a case of redo ablation of long-standing persistent atrial fibrillation in which the left atrial appendage played a key role in maintaining AF during ablation, and atrial fibrillation was terminated by electrical isolation of the LAA. During the ablation, a rare phenomenon of half of the atria in atrial fibrillation while the other half of the atria in atrial flutter was seen.
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http://dx.doi.org/10.1155/2020/9691584DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321523PMC
June 2020

Characteristics and clinical significance of myocardial injury in patients with severe coronavirus disease 2019.

Eur Heart J 2020 06;41(22):2070-2079

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

Aims: To investigate the characteristics and clinical significance of myocardial injury in patients with severe coronavirus disease 2019 (COVID-19).

Methods And Results: We enrolled 671 eligible hospitalized patients with severe COVID-19 from 1 January to 23 February 2020, with a median age of 63 years. Clinical, laboratory, and treatment data were collected and compared between patients who died and survivors. Risk factors of death and myocardial injury were analysed using multivariable regression models. A total of 62 patients (9.2%) died, who more often had myocardial injury (75.8% vs. 9.7%; P < 0.001) than survivors. The area under the receiver operating characteristic curve of initial cardiac troponin I (cTnI) for predicting in-hospital mortality was 0.92 [95% confidence interval (CI), 0.87-0.96; sensitivity, 0.86; specificity, 0.86; P < 0.001]. The single cut-off point and high level of cTnI predicted risk of in-hospital death, hazard ratio (HR) was 4.56 (95% CI, 1.28-16.28; P = 0.019) and 1.25 (95% CI, 1.07-1.46; P = 0.004), respectively. In multivariable logistic regression, senior age, comorbidities (e.g. hypertension, coronary heart disease, chronic renal failure, and chronic obstructive pulmonary disease), and high level of C-reactive protein were predictors of myocardial injury.

Conclusion: The risk of in-hospital death among patients with severe COVID-19 can be predicted by markers of myocardial injury, and was significantly associated with senior age, inflammatory response, and cardiovascular comorbidities.
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http://dx.doi.org/10.1093/eurheartj/ehaa408DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239100PMC
June 2020

Association of Cardiac Injury With Mortality in Hospitalized Patients With COVID-19 in Wuhan, China.

JAMA Cardiol 2020 07;5(7):802-810

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China.

Importance: Coronavirus disease 2019 (COVID-19) has resulted in considerable morbidity and mortality worldwide since December 2019. However, information on cardiac injury in patients affected by COVID-19 is limited.

Objective: To explore the association between cardiac injury and mortality in patients with COVID-19.

Design, Setting, And Participants: This cohort study was conducted from January 20, 2020, to February 10, 2020, in a single center at Renmin Hospital of Wuhan University, Wuhan, China; the final date of follow-up was February 15, 2020. All consecutive inpatients with laboratory-confirmed COVID-19 were included in this study.

Main Outcomes And Measures: Clinical laboratory, radiological, and treatment data were collected and analyzed. Outcomes of patients with and without cardiac injury were compared. The association between cardiac injury and mortality was analyzed.

Results: A total of 416 hospitalized patients with COVID-19 were included in the final analysis; the median age was 64 years (range, 21-95 years), and 211 (50.7%) were female. Common symptoms included fever (334 patients [80.3%]), cough (144 [34.6%]), and shortness of breath (117 [28.1%]). A total of 82 patients (19.7%) had cardiac injury, and compared with patients without cardiac injury, these patients were older (median [range] age, 74 [34-95] vs 60 [21-90] years; P < .001); had more comorbidities (eg, hypertension in 49 of 82 [59.8%] vs 78 of 334 [23.4%]; P < .001); had higher leukocyte counts (median [interquartile range (IQR)], 9400 [6900-13 800] vs 5500 [4200-7400] cells/μL) and levels of C-reactive protein (median [IQR], 10.2 [6.4-17.0] vs 3.7 [1.0-7.3] mg/dL), procalcitonin (median [IQR], 0.27 [0.10-1.22] vs 0.06 [0.03-0.10] ng/mL), creatinine kinase-myocardial band (median [IQR], 3.2 [1.8-6.2] vs 0.9 [0.6-1.3] ng/mL), myohemoglobin (median [IQR], 128 [68-305] vs 39 [27-65] μg/L), high-sensitivity troponin I (median [IQR], 0.19 [0.08-1.12] vs <0.006 [<0.006-0.009] μg/L), N-terminal pro-B-type natriuretic peptide (median [IQR], 1689 [698-3327] vs 139 [51-335] pg/mL), aspartate aminotransferase (median [IQR], 40 [27-60] vs 29 [21-40] U/L), and creatinine (median [IQR], 1.15 [0.72-1.92] vs 0.64 [0.54-0.78] mg/dL); and had a higher proportion of multiple mottling and ground-glass opacity in radiographic findings (53 of 82 patients [64.6%] vs 15 of 334 patients [4.5%]). Greater proportions of patients with cardiac injury required noninvasive mechanical ventilation (38 of 82 [46.3%] vs 13 of 334 [3.9%]; P < .001) or invasive mechanical ventilation (18 of 82 [22.0%] vs 14 of 334 [4.2%]; P < .001) than those without cardiac injury. Complications were more common in patients with cardiac injury than those without cardiac injury and included acute respiratory distress syndrome (48 of 82 [58.5%] vs 49 of 334 [14.7%]; P < .001), acute kidney injury (7 of 82 [8.5%] vs 1 of 334 [0.3%]; P < .001), electrolyte disturbances (13 of 82 [15.9%] vs 17 of 334 [5.1%]; P = .003), hypoproteinemia (11 of 82 [13.4%] vs 16 of 334 [4.8%]; P = .01), and coagulation disorders (6 of 82 [7.3%] vs 6 of 334 [1.8%]; P = .02). Patients with cardiac injury had higher mortality than those without cardiac injury (42 of 82 [51.2%] vs 15 of 334 [4.5%]; P < .001). In a Cox regression model, patients with vs those without cardiac injury were at a higher risk of death, both during the time from symptom onset (hazard ratio, 4.26 [95% CI, 1.92-9.49]) and from admission to end point (hazard ratio, 3.41 [95% CI, 1.62-7.16]).

Conclusions And Relevance: Cardiac injury is a common condition among hospitalized patients with COVID-19 in Wuhan, China, and it is associated with higher risk of in-hospital mortality.
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http://dx.doi.org/10.1001/jamacardio.2020.0950DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7097841PMC
July 2020

Long non-coding RNA LEF1-AS1 is involved in the progression of retinoblastoma through regulating the Wnt/β-catenin pathway.

Authors:
Hua He Mu Qin

Clin Exp Pharmacol Physiol 2020 05 21;47(5):886-891. Epub 2020 Feb 21.

Department of Ophthalmology, Affiliated Hospital of Xiangnan University, Chenzhou, China.

The lymphoid enhancer binding factor 1 antisense RNA 1 (LEF1-AS1) has been suggested to function as a tumour-associated lncRNA in several types of human cancers, but there is no study to date about the role of LEF1-AS1 in retinoblastoma. In our study, LEF1-AS1 expression was increased in retinoblastoma tissues and cell lines compared with paired adjacent normal tissues and the retinal pigment epithelial cell line, respectively. Meanwhile, we found that patients with retinoblastoma with IIRC D-E or undifferentiated type had notably higher levels of LEF1-AS1 expression than those with IIRC A-C or differentiated type. High LEF1-AS1 expression predicted poor disease-free survival in patients with retinoblastoma. The in vitro assays suggested that silencing of LEF1-AS1 suppressed retinoblastoma cell proliferation, migration, and invasion through regulating the Wnt/β-catenin pathway. In conclusion, LEF1-AS1 functions as an oncogenic lncRNA in retinoblastoma.
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http://dx.doi.org/10.1111/1440-1681.13263DOI Listing
May 2020

Osteopontin induces atrial fibrosis by activating Akt/GSK-3β/β-catenin pathway and suppressing autophagy.

Life Sci 2020 Mar 15;245:117328. Epub 2020 Jan 15.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Aims: Atrial fibrosis is a common feature of atrial fibrillation (AF). Recently, it is reported that osteopontin (OPN) can induce fibrosis in lungs, livers and kidneys. However, its role in atrial fibrosis remains unclear. Here, we sought to determine the involvement of OPN in atrial fibrosis and the underlying mechanisms during this pathological remodeling process.

Materials And Methods: Protein expressions were determined by enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining and immunoblotting. mRNA expressions were detected by qRT-PCR. Cell proliferation was assessed by CCK-8. Left atrial electroanatomical voltage maps were created using PentaRay catheters and a 3-dimensional mapping system.

Key Findings: OPN was highly expressed in the circulation of AF patients and was further increased with the progression of AF. In addition, correlation analysis showed that circulating OPN positively related with low-voltage areas (LVAs, a marker of atrial fibrosis) in AF patients. Immunohistological staining and immunoblotting revealed an increased expression of OPN in AF patients who present a higher degree of atrial fibrosis. Furthermore, in vitro studies in cultured human atrial fibroblasts (hAFs) demonstrated that OPN promoted the proliferation of fibroblasts and increased production of collagen I and fibronectin. Mechanistically, the profibrotic effects of OPN on atrial fibroblasts were determined via activating Akt/GSK-3β/β-catenin signaling and suppressing autophagy.

Significance: This study uncovered a previously unrecognized profibrotic role of OPN in atrial fibrosis, which was achieved through activation of Akt/GSK-3β/β-catenin signaling pathway and suppression of autophagy, implying a promising therapeutic target in atrial fibrosis and AF.
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http://dx.doi.org/10.1016/j.lfs.2020.117328DOI Listing
March 2020

Electrogram dispersion-guided driver ablation adjunctive to high-quality pulmonary vein isolation in atrial fibrillation of varying durations.

J Cardiovasc Electrophysiol 2020 01 20;31(1):48-60. Epub 2019 Nov 20.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.

Objective: To investigate the role of driver mechanism and the effect of electrogram dispersion-guided driver mapping and ablation in atrial fibrillation (AF) at different stages of progression.

Methods: A total of 256 consecutive patients with AF who had undergone pulmonary vein isolation (PVI) plus driver ablation or conventional ablation were divided into three groups: paroxysmal atrial fibrillation (PAF; group A, n = 51); persistent atrial fibrillation (PsAF; group B, n = 38); and long standing-persistent atrial fibrillation (LS-PsAF; group C, n = 39). PVI was performed with the guidance of the ablation index. The electrogram dispersion was analyzed for driver mapping.

Results: The most prominent driver regions were at roof (28.0%), posterior wall (17.6%), and bottom (21.3%). From patients with PAF to those with PsAF and LS-PsAF: the complexity of extra-pulmonary vein (PV) drivers including distribution, mean number, and area of dispersion region increased (P < .001). Patients who underwent driver ablation vs conventional ablation had higher procedural AF termination rate (76.6% vs 28.1%; P < .001). With AF progression, the termination rate gradually decreased from group A to group C, and the role of PVI in AF termination was also gradually weakened from group A to group C (39.6%, 7.4%, and 4.3%; P < .001) in patients with driver ablation. At the end of the follow-up, the rate of sinus rhythm maintenance was higher in patients with driver ablation than those with conventional ablation (89.1% vs 70.3%; P < .001).

Conclusion: The formation of extra-PV drivers provides an important mechanism for AF maintenance with their complexity increasing with AF progression. Electrogram dispersion-guided driver ablation appears to be an efficient adjunctive approach to PVI for AF treatment.
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http://dx.doi.org/10.1111/jce.14268DOI Listing
January 2020

Identification and characterization of cherry (Cerasus pseudocerasus G. Don) genes responding to parthenocarpy induced by GA3 through transcriptome analysis.

BMC Genet 2019 08 1;20(1):65. Epub 2019 Aug 1.

College of Horticulture Science and Engineering, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, China.

Background: Fruit set after successful pollination is key for the production of sweet cherries, and a low fruit-setting rate is the main problem in production of this crop. As gibberellin treatment can directly induce parthenogenesis and satisfy the hormone requirement during fruit growth and development, such treatment is an important strategy for improving the fruit-setting rate of sweet cherries. Previous studies have mainly focused on physiological aspects, such as fruit quality, fruit size, and anatomical structure, whereas the molecular mechanism remains clear.

Results: In this study, we analyzed the transcriptome of 'Meizao' sweet cherry fruit treated with gibberellin during the anthesis and hard-core periods to identify genes associated with parthenocarpic fruit set. A total of 25,341 genes were identified at the anthesis and hard-core stages, 765 (681 upregulated, 84 downregulated) and 186 (141 upregulated, 45 downregulated) of which were significant differentially expressed genes (DEGs) at the anthesis and the hard-core stages after gibberellin 3 (GA3) treatment, respectively. Based on DEGs between the control and GA3 treatments, the GA3 response mainly involves parthenocarpic fruit set and cell division. Exogenous gibberellin stimulated sweet cherry fruit parthenocarpy and enlargement, as verified by qRT-PCR results of related genes as well as the parthenocarpic fruit set and fruit size. Based on our research and previous studies in Arabidopsis thaliana, we identified key genes associated with parthenocarpic fruit set and cell division. Interestingly, we observed patterns among sweet cherry fruit setting-related DEGs, especially those associated with hormone balance, cytoskeleton formation and cell wall modification.

Conclusions: Overall, the result provides a possible molecular mechanism regulating parthenocarpic fruit set that will be important for basic research and industrial development of sweet cherries.
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http://dx.doi.org/10.1186/s12863-019-0746-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670208PMC
August 2019

Long-Term Effect of Different Optimizing Methods for Cardiac Resynchronization Therapy in Patients with Heart Failure: A Randomized and Controlled Pilot Study.

Cardiology 2019;142(3):158-166. Epub 2019 Jun 12.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Aim: During cardiac resynchronization therapy (CRT), optimized programming of the atrioventricular (AV) delay and ventricular-to-ventricular (VV) interval can lead to improved hemodynamics, symptomatic response, and left ventricular systolic function. Currently, however, there is no recommendation for the best optimization method. This study aimed to compare the long-term clinical outcomes of 4 different CRT optimization methods.

Methods: One hundred and twenty-four consecutive CRT patients with severe heart failure and left bundle-branch block configuration were randomly assigned into four groups to undergo AV/VV delay optimization through echocardiogram (ECHO; n = 30), electrocardiogram (ECG; n = 32), QuickOpt algorithm (n = 28), and nominal AV/VV (n = 36) groups. Patients were followed up and underwent examinations, including New York Heart Association (NYHA) cardiac functional classification, 6-min walking distance (6MWD), and echocardiography, at 6, 12, 24, 36, and 48 months, respectively. The patients' survival and clinical outcomes were compared among the four groups.

Results: Kaplan-Meier survival analyses showed that the median survival was the same in the 4 groups: ECHO, 43 months; ECG, 44 months; QuickOpt, 44 months, and nominal, 41 months. At the 6-month follow-up, the reduction in left ventricular end diastolic diameter (LVEDD) was significantly less in the nominal group (-1.91 ± 2.58 mm) than that in the other three groups (ECHO: -3.70 ± 2.78 mm, p = 0.012; ECG: -3.53 ± 3.14 mm, p = 0.020; QuickOpt: -3.46 ± 2.65 mm, p = 0.032); 6MWD was significantly shorter in the nominal group (87.88 ± 34.76 m) than that in the other three groups (ECHO: 120.63 ± 56.93 m, p = 0.006; ECG: 114.97 ± 54.95 m, p = 0.020; QuickOpt: 114.57 ± 35.41 m, p = 0.027). Left ventricular ejection fraction (LVEF) significantly increased in ECHO (7.23 ± 2.76%, p = 0.010), ECG (8.50 ± 3.17%, p < 0.001), and QuickOpt (8.39 ± 2.90%, p < 0.001) compared with the nominal group (5.35 ± 2.59%). There were no significant differences among the groups in the aforementioned parameters at 24, 36, and 48 months, respectively.

Conclusion: While LVEDD, LVEF, 6MWD, and NYHA were significantly improved in ECHO, ECG, and QuickOpt at 6 months, there were no significant improvements in any of the groups at 12, 24, and 48 months. These findings suggested that the long-term effect of the four CRT methods for heart failure was not significantly different.
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http://dx.doi.org/10.1159/000499502DOI Listing
December 2019

The cardiac autonomic nervous system: A target for modulation of atrial fibrillation.

Clin Cardiol 2019 Jun 6;42(6):644-652. Epub 2019 May 6.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.

The cardiac autonomic nerve system (CANS) is a potentially potent modulator of the initiation and perpetuation of atrial fibrillation (AF). In this review, we focus on the relationship between the autonomic nervous system (ANS) and the pathophysiology of AF and the potential benefit and limitations of neuromodulation in the management of this arrhythmia from eight aspects. We conclude that Activation and Remodeling of CANS involved in the initiation and maintenance of AF. The network control mechanism, innervation regions, and sympathetic/parasympathetic balance play an important role in AF substrate. And the formation of Complex Fractional Atrial Electrograms also related to CANS activity. In addition, modulating CANS function by potential therapeutic applications include ganglionated plexus ablation, renal sympathetic denervation, and low-level vagal nerve stimulation, may enable AF to be controlled. Although the role of the ANS has long been recognized, a better understanding of the complex interrelationships of the various components of the CANS will lead to improvement of treatments for this common arrhythmia.
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http://dx.doi.org/10.1002/clc.23190DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6553352PMC
June 2019

Integrative Analysis Reveals Key Circular RNA in Atrial Fibrillation.

Front Genet 2019 19;10:108. Epub 2019 Feb 19.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China.

Circular RNAs (circRNAs) are an emerging class of RNA species that may play a critical regulatory role in gene expression control, which can serve as diagnostic biomarkers for many diseases due to their abundant, stable, and cell- or tissue-specific expression. However, the association between circRNAs and atrial fibrillation (AF) is still not clear. In this study, we used RNA sequencing data to identify and quantify the circRNAs. Differential expression analysis of the circRNAs identified 250 up- and 126 down-regulated circRNAs in AF subjects compared with healthy donors, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the parental genes of the dysregulated circRNAs indicated that the up-regulated parental genes may participate in the process of DNA damage under oxidative stress. Furthermore, to annotate the dysregulated circRNAs, we constructed and merged the competing endogenous RNA (ceRNA) network and protein-protein interaction (PPI) network, respectively. In the merged network, 130 of 246 dysregulated circRNAs were successfully characterized by more than one pathway. Notably, the five circRNAs, including chr9:15474007-15490122, chr16:75445723-75448593, hsa_circ_0007256, chr12:56563313-56563992, and hsa_circ_0003533, showed the highest significance by the enrichment analysis, and four of them were enriched in cytokine-cytokine receptor interaction. These dysregulated circRNAs may mainly participate in biological processes of inflammatory response. In conclusion, the present study identified a set of dysregulated circRNAs, and characterized their potential functions, which may be associated with inflammatory responses in AF. To our knowledge, this is the first study to uncover the association between circRNAs and AF, which not only improves our understanding of the roles of circRNAs in AF, but also provides candidates of potentially functional circRNAs for AF researchers.
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http://dx.doi.org/10.3389/fgene.2019.00108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6389718PMC
February 2019

Regulation of Atrial Fibrosis by the Bone.

Hypertension 2019 02;73(2):379-389

National Clinical Research Center for Oral Diseases, China (X.-N.S., L.-J.D., Yuan Liu, Yan Liu, J.-Y.S., Z.T., M.X., B.F., S.-Z.D.).

MR (mineralocorticoid receptor) antagonists have been demonstrated to provide beneficial effects on preventing atrial fibrosis. However, the underlying cellular and molecular mechanisms remain unclear. We aim to determine the role of osteoblast MR in atrial fibrosis and to explore the underlying mechanism. Using osteoblast MR knockout mouse in combination with mutant TGF (transforming growth factor)-β1 transgenic mouse, we demonstrated that MR deficiency in osteoblasts significantly attenuated atrial fibrosis. Mechanistically, MR directly regulated expression of OCN (osteocalcin) in osteoblasts. Both carboxylated and undercarboxylated OCNs (ucOC) were less secreted in osteoblast MR knockout mice. Mutant TGF-β1 transgenic mice supplemented with recombinant ucOC showed aggravated atrial fibrosis. In cultured atrial fibroblasts, ucOC treatment promoted proliferation and migration of atrial fibroblasts, whereas cotreatment with an antagonist for a GPRC6A (G-protein-coupled receptor, family C, group 6, member A) abolished these effects. Western blotting analysis revealed upregulation of PKA (protein kinase A) and CREB (cAMP-response element-binding protein) phosphorylation after ucOC treatment. Inhibition of PKA with its antagonist reduced ucOC-induced proliferation and migration of atrial fibroblasts. Finally, the impact of osteoblast MR deficiency on atrial fibrosis was abolished by ucOC administration in mutant TGF-β1 transgenic mice. Taken together, MR deficiency in osteoblasts attenuated atrial fibrosis by downregulation of OCN to promote proliferation and migration of atrial fibroblasts.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.118.11544DOI Listing
February 2019

Dispersion-guided ablation in conjunction with circumferential pulmonary vein isolation is superior to stepwise ablation approach for persistent atrial fibrillation.

Int J Cardiol 2019 Mar 19;278:97-103. Epub 2018 Dec 19.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Background: Due to the lack of optimal ablation strategy, the success rate of persistent atrial fibrillation (AF) is still low. We hypothesize that a strategy that targeting pulmonary triggers and dispersion areas in atria improves prognosis of persistent AF.

Methods: We prospectively enrolled 142 persistent AF patients admitted for catheter ablation. These patients were randomly assigned in a 1:1 ratio to ablation with circumferential pulmonary vein isolation (CPVI) + ablation of electrogram dispersion areas (71 patients, group A) or stepwise ablation strategy (71 patients, group B).

Results: Procedural time and fluoroscopy time did not differ between group A and group B (204.6 ± 26.9 min vs 207.8 ± 26.3 min and 7.3 ± 1.3 min vs 7.1 ± 1.3 min, respectively, P > 0.05), however, radiofrequency delivery time in group A was significantly shorter than that in group B (70 ± 7.2 min vs 83.2 ± 9.1 min, P < 0.001). In total, 265 electrogram dispersion areas were identified in 67 patients, and the most prominent areas were roof, bottom, and inferoposterior wall. The rates of acute AF endpoint (including AF termination and AFCL elongation >30 ms) and termination in group A were significantly higher than that in group B (97.2% vs. 71.8% and 70.4% vs. 15.5%, respectively, P < 0.001). During a follow-up period of 204 ± 67 days, both AF-free and AF/AT-free survival in group A were significantly higher than that in group B (P = 0.012 and P = 0.014, respectively).

Conclusion: Dispersion-guided ablation in conjunction with CPVI is efficient, personalized, and accurate for persistent AF.
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http://dx.doi.org/10.1016/j.ijcard.2018.12.051DOI Listing
March 2019

Extra pulmonary vein driver mapping and ablation in paroxysmal atrial fibrillation by electrogram dispersion analysis.

J Cardiovasc Electrophysiol 2019 02 21;30(2):164-170. Epub 2018 Nov 21.

Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai, China.

Background: The adjunctive approach is still unknown for atrial fibrillation (AF), which cannot be terminated after pulmonary vein isolation (PVI). We hypothesized that the driver ablation plus PVI was superior to PVI alone.

Methods And Results: A total of 98 patients with paroxysmal AF were enrolled in this study and were divided into two groups, with one group undergoing PVI (n = 49) and the other group undergoing PVI + driver ablation (n = 49). The driver regions were defined as clusters of bipolar electrograms that displayed spatial dispersion spread over mean AF cycle length at a minimum of three adjacent bipolars of a PentaRay catheter. During the procedure, the most prominent driver regions before PVI were the roof (n = 27; 55.1%), PV antrum (n = 23; 46.9%), and the inferoposterior wall (n = 11; 22.4%). PVI can eliminate all drivers at PV antrum, but only terminate 30.4% of AF in the driver group. The AF termination rate in the driver ablation group was significantly higher than that in conventional ablation (93.9% vs 40.6%; P < 0.001). The rate of freedom from atrial tachyarrhythmia episodes by a single procedure at 6 months was significantly higher in the driver group than in the conventional group (91.6% vs 72.4%; P = 0.02).

Conclusion: The present method is effective for AF driver identification. It guided ablation adjunctive to PVI increasing the rate of AF termination and improving the outcomes in patients with paroxysmal AF.
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http://dx.doi.org/10.1111/jce.13784DOI Listing
February 2019

Characterization of MK₈(H₂) from sp. B7740 and Its Potential Antiglycation Capacity Measurements.

Mar Drugs 2018 Oct 18;16(10). Epub 2018 Oct 18.

Natural Product Laboratory, Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK₂), which is mainly produced through the fermentation of microorganisms. MK₈(H₂) was identified to be the main menaquinone from sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK₈(H₂) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK₈(H₂) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK₈(H₂) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK₄. High-performance liquid chromatography⁻mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK₈(H₂) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK₈(H₂) a promising natural active ingredient with future food and medicine applications.
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http://dx.doi.org/10.3390/md16100391DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213960PMC
October 2018

Atrial Ganglionated Plexus Modification: A Novel Approach to Treat Symptomatic Sinus Bradycardia.

JACC Clin Electrophysiol 2017 09 26;3(9):950-959. Epub 2017 Apr 26.

Department of Medicine and Heart Rhythm Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma.

Objectives: This study sought to determine if anatomic atrial ganglionated plexus (GP) ablation leads to long-term sinus rate (SR) increase and improves quality of life in patients with symptomatic sinus bradycardia (SB).

Background: Atrial GP ablation has been demonstrated to increase SR in our previous study. Atrial GP ablation may also be effective in treating patients with symptomatic SB.

Methods: Sixty-two patients with symptomatic SB were recruited: Group A included patients <50 years of age (n = 40); Group B included patients ≥50 years of age (n = 22). All patients underwent anatomic ablation of the main atrial GP, and 24-h Holter monitoring and quality-of-life assessment were performed during 1 year of follow-up. Quality of life was accessed by the Medical Outcomes Study Short-Form 36 Health Survey.

Results: Although SR markedly increased in all patients after GP ablation, the increase was significantly greater in patients <50 years of age than in patients ≥50 years of age (19.3 ± 9.9 beats/min vs. 10.8 ± 5.4 beats/min; p = 0.001). The right anterior GP and the GP at the junction of the aorta and superior vena cava made the greatest contributions to SR increase among all GP. The mean and minimal SR increased significantly after ablation and remained elevated for 12 months only in Group A patients. Although symptoms and quality of life improved in all patients, 5 of the 8 domains of the Medical Outcomes Study Short-Form 36 Health Survey did not show obvious improvements in patients of Group B at 12 months.

Conclusions: Anatomic atrial GP ablation effectively increased SR and improved quality of life in patients <50 years of age with symptomatic SB.
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http://dx.doi.org/10.1016/j.jacep.2017.01.022DOI Listing
September 2017
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