Publications by authors named "Mostafa Sadeghi"

67 Publications

Giant lipoma pediculated in the falciform ligament: A case report.

Caspian J Intern Med 2021 ;12(Suppl 2):S471-S473

Endoscopic and Minimally Invasive Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

Background: The lipoma is one of the benign soft tissue tumors that occur most in adulthood. These tumors are one of the common tumors of the limbs, and rarely occur in the abdominal cavity. These masses usually grow slowly and are asymptomatic.

Case Presentation: In this article, we present a 23-year-old woman who was referred to a doctor with complaints of abdominal pain and enlargement that occurred in the last 4 months. Following surgery and sampling, it was found that the patient had a lipoma, and the liver was a phlegmatic liver in the liver form.

Conclusion: The recurrence and metastasis of the primary tumor histology are not always predictable, and the authors stated that all cases of individual fibrous tumors should be known as potentially malignant.
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http://dx.doi.org/10.22088/cjim.12.0.471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8559652PMC
January 2021

Characteristics of Emergency Medical Service Missions in Out-of-Hospital Cardiac Arrest and Death Cases in the Periods of Before and After the COVID-19 Pandemic.

Prehosp Disaster Med 2021 Dec 8;36(6):676-683. Epub 2021 Oct 8.

Prehospital and Hospital Emergency Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: Some studies in countries affected by the coronavirus disease of 2019 (COVID-19) pandemic have shown that the missions of Emergency Medical Service (EMS) have changed during the COVID-19 pandemic, and the rate of death and out-of-hospital cardiac arrest (OHCA) has been increased due to the direct and indirect effects of COVID-19.

Objective: The aim of this study was to determine the effect of the COVID-19 pandemic on the process of EMS missions, death, and OHCA.

Methods: This cross-sectional study was performed in Tehran, Iran. All conducted missions in the first six months of the three consecutive solar years of March 21 until September 22 of 2018-2020, which were registered in the registry bank of the Tehran EMS center, were assessed and compared. Based on the opinion of experts, the technician's on-scene diagnoses were categorized into 14 groups, and then death and OHCA cases were compared.

Results: In this study, the data of 1,050,376 missions performed in three study periods were analyzed. In general, the number of missions in 2020 was 17.83% fewer than that of 2019 (P < .001); however, the number of missions in 2019 was 30.33% more than that of 2018. On the other hand, the missions of respiratory problems, cardiopulmonary arrest, infectious diseases, and poisoning were increased in 2020 compared to that of 2019. The raw number of OHCA and death cases respectively in 2018, 2019, and 2020 were 25.0, 22.7, and 28.6 cases per 1,000 missions. Of all patients who died in 2020, 4.9% were probable/confirmed COVID-19 cases. The history of heart disease, hypertension, diabetes, and respiratory disease in patients in 2020 was more frequent than that of the other two years.

Conclusion: This study showed that the number of missions in the Tehran EMS in 2020 were decreased compared to that of 2019, however the number of missions in 2019 was more than that of 2018. Respiratory problems, infectious diseases, poisoning, death, and OHCA were increased compared to the previous two years and cardiovascular complaints, neurological problems, and motor vehicle collisions (MVCs) in 2020 were fewer than that of the other two years.
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http://dx.doi.org/10.1017/S1049023X21001138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8529353PMC
December 2021

Effects of coenzyme Q10 on reproductive performance of laying Japanese quail (Coturnix japonica) under cadmium challenge.

Poult Sci 2021 Nov 8;100(11):101418. Epub 2021 Aug 8.

Department of Poultry Science, University of Georgia, Athens, GA 30602-2772, USA.

Japanese quail is an increasingly important bird of economic importance for commercial egg and meat production, particularly in developing countries. There is a need for research aimed at improving efficiency of these birds during stressful challenges, such as oxidative stress. Coenzyme Q10 (CoQ10), a highly functional antioxidant, protects cells against oxidative stress. This study was conducted to determine the effects of CoQ10 on reproductive performance of Japanese quail under cadmium (Cd) challenge. A total of 216 six-wk-old Japanese quail were randomly allocated into 3 groups for an 8 wk experimental trial. The treatments include a negative control (NC): feeding basal diet; a positive control (PC): feeding basal diet and cadmium administration (1 mg/100 g BW, at 10 and 11 wk of age), and (CdQ10): feeding CoQ10 supplemented (900 mg/kg diet) basal diet and Cd administration. At 11 and 13 wk of age, egg production, body weight, mortality, oviduct, and ovarian biometry, were recorded. Histology and histopathology of isthmus and magnum, fertility, hatchability, hatchling quality, and HSP70 mRNA transcript abundance in the utero-vaginal junction (UVJ) were evaluated. Positive control and CdQ10 group had no significant effect on live body weight, stroma weight, follicle size, hatchability, and fertility; however, Cd administration increased (P < 0.01) mortality rate in the PC group compared to the NC and CdQ10 groups. CdQ10 quail produced more eggs and had a higher hatchling quality compared to the PC group (P < 0.01). The thickness and height of isthmus and magnum folds in the CdQ10 group was increased compared to the PC group (P < 0.01) and overall oviduct weight was increased with CoQ10 supplementation (P < 0.01). Compared to PC, the CdQ10 group had a reduction in infiltration of inflammatory cells. Relative abundance of HSP70 mRNA in UVJ was influenced by interactive effect of treatment × time (P < 0.05). In conclusion, dietary supplementation of CoQ10 showed beneficial effects on some reproduction characteristics of female Japanese quail under Cd-induced oxidative stress.
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http://dx.doi.org/10.1016/j.psj.2021.101418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8531857PMC
November 2021

Effects of zinc dosage and particle size on gut morphology, tight junctions and TNF-α expression in broiler breeder hens.

J Anim Physiol Anim Nutr (Berl) 2021 Sep 12. Epub 2021 Sep 12.

Department of Animal Science, University of Tehran, Karaj, Iran.

This study was performed to evaluate the effects of different amounts and particle size of zinc oxide (ZnO) on villus height (VH), villus width (VW), crypt depth (CD) and VH to CD ratio (VH: CD), and expression of zonula occludens-1 (ZO-1), occludin (OC) and tumour necrosis factor-α (TNF-α) in broiler breeders. A total of 350 (Ross 308) broiler breeder hens of 54 weeks randomly assigned to seven treatments, included control basal diet (C) without added Zn, C+ 100, and 130 mg Zn per kg of diet from Large (L) (100-1000 nm) and Small (S) (<100 nm) particle size ZnO (LZnO100 and 130; SZnO100 and 130), C and SZnO100 challenged with lipopolysaccharide (C+LPS and SZnO100+LPS). Each diet was fed to five replicates consisting of ten birds each. The middle part of the duodenum, jejunum and ileum was used for morphological assessments. To assess the gene expression of ZO-1, OC and TNF-α in the jejunum samples were excised. Results showed that the supplementing 130 ppm SZnO increased VH:CD in the duodenum (p < 0.05). VW in the duodenum and all the evaluated morphometric indices in jejunum and ileum were not affected by the dietary treatment (p > 0.05). ZO-1 mRNA abundance in C+LPS group compared to SZnO100+LPS group was significantly decreased and increased by LPS and SZnO100 respectively. The SZnO-100 increased OC gene expression in compare to C+LPS group. The expression of TNF-α in C+LPS treatment was higher than other groups (p < 0.05). The lowest and the highest litter moisture and foot-pad dermatitis (FPD) were observed in LZnO-130 and C treatments respectively (p < 0.05). Improving the physical properties of ZnO affect on VH:CD. Broiler breeder diet with ZnO enhance ZO-1, OC and mitigate TNF-α gene expression in jejunum maintenance of gut health in broiler breeders.
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http://dx.doi.org/10.1111/jpn.13638DOI Listing
September 2021

Omics Multi-Layers Networks Provide Novel Mechanistic and Functional Insights Into Fat Storage and Lipid Metabolism in Poultry.

Front Genet 2021 7;12:646297. Epub 2021 Jul 7.

One Health at UCalgary, University of Calgary, Calgary, AB, Canada.

Fatty acid metabolism in poultry has a major impact on production and disease resistance traits. According to the high rate of interactions between lipid metabolism and its regulating properties, a holistic approach is necessary. To study omics multilayers of adipose tissue and identification of genes and miRNAs involved in fat metabolism, storage and endocrine signaling pathways in two groups of broiler chickens with high and low abdominal fat, as well as high-throughput techniques, were used. The gene-miRNA interacting bipartite and metabolic-signaling networks were reconstructed using their interactions. In the analysis of microarray and RNA-Seq data, 1,835 genes were detected by comparing the identified genes with significant expression differences (p.adjust < 0.01, fold change ≥ 2 and ≤ -2). Then, by comparing between different data sets, 34 genes and 19 miRNAs were detected as common and main nodes. A literature mining approach was used, and seven genes were identified and added to the common gene set. Module finding revealed three important and functional modules, which were involved in the peroxisome proliferator-activated receptor (PPAR) signaling pathway, biosynthesis of unsaturated fatty acids, Alzheimer's disease metabolic pathway, adipocytokine, insulin, PI3K-Akt, mTOR, and AMPK signaling pathway. This approach revealed a new insight to better understand the biological processes associated with adipose tissue.
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http://dx.doi.org/10.3389/fgene.2021.646297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8292821PMC
July 2021

The Possible Factors Correlated with The Higher Risk of Getting Infected by COVID-19 in Emergency Medical Technicians; A Case-Control Study.

Bull Emerg Trauma 2021 Apr;9(2):67-72

School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Objective: To assess the possible factors associated with increasing risk of COVID-19 among EMTs.

Methods: This study was a case-control study conducted in Tehran, Iran. Case group was consisted of confirmed COVID-19 EMTs based on the results of reverse transcriptase polymerase chain reaction and/or lung computed tomography scan. Healthy EMTs were randomly selected as control group. Patients were asked to fill out a checklist including demographic data, data related to the work situation (such as number of missions and type of mask and cloth) and PPE precautions.

Results: Sixty-eight patients and 148 healthy persons took part in this study as case and control group, respectively. Having two EMTs involved directly in taking care of patients (<0.001) and working with a confirmed case teammate (<0.001), considering the precautions such as seal check after wearing the mask (=0.015), covering the hair with a medical hat (<0.001), not using personal items despite protective clothing (<0.001), and avoiding contact with the outer surface of clothing while removing (<0.001) had significant difference in two groups.

Conclusion: We found that the type and method of use of PPE were correlated with the increasing risk of COVID-19 in EMTs. Also, we found that when two EMTs were involved directly in taking care of the patients, and those who worked with a confirmed case teammate, more frequently affected.
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http://dx.doi.org/10.30476/BEAT.2021.89713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8195834PMC
April 2021

The Geographical Distribution of Probable COVID-19 Patients Transferred by Tehran Emergency Medical Services; a Cross Sectional Study.

Arch Acad Emerg Med 2021 10;9(1):e25. Epub 2021 Mar 10.

Prehospital and Hospital Emergency Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Introduction: Mapping of COVID-19 infection in the city can help us know more about how the disease is distributed and spread. This study was conducted to investigate the geographical distribution of probable COVID-19 patients who were transferred to destination hospitals by emergency medical services (EMS) in the first wave of the epidemic, in Tehran, Iran.

Methods: This cross-sectional study was performed based on recorded missions during the first 3-month period of the pandemic in Tehran, Iran. All probable cases of COVID-19 who were transferred to the hospitals following contact with Tehran EMS during the study period were enrolled. Arc-GIS software was utilized to draw the distribution map of the contact places of the cases.

Results: In this study, the data of 4018 patients were analyzed (60.9% male). The mean age of the patients was 54.1 ± 20.7 years; and the mean age of the patients had increased with time during the studied 3 months (p = 0.003). The average incidence rate of this disease in Tehran during the study period was 4.6 per 10,000 population. Generally, the lowest and highest raw frequencies of Tehran COVID-19 contamination were seen in municipal districts 21 and 4, respectively. The raw frequency of cases during the 3-month study period also showed that the highest number of cases in Tehran occurred in municipal districts 5 and 4, respectively.

Conclusion: In the present study, using geographic information systems (GIS), geographical distribution map of COVID-19 in Tehran, Iran, during the first 3 months of the pandemic was drawn.
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http://dx.doi.org/10.22037/aaem.v9i1.1177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126355PMC
March 2021

Microsatellite Analysis of Genetic Diversity and Population Structure of the Iranian Kurdish Horse.

J Equine Vet Sci 2021 03 16;98:103358. Epub 2020 Dec 16.

College of Animal Sciences Guangxi University, Nanning, China.

Native breeds are essential for national stocks and genetic reservoir; therefore, the preservation of indigenous breeds is a key policy priority for countries around the world. Many conservationists would assert that genetic diversity is a prerequisite for adaptive evolution, and preserving genetic diversity will need conservation efforts for the long-term survival of domestic species. This study intended to evaluate the genetic diversity of the Iranian Kurdish horse population based on microsatellite indicators, which can partially prevent it from becoming extinct. Fifty-eight tail hair and blood samples were randomly collected from Kurdistan, Kermanshah, Ilam, West Azerbaijan, Isfahan, Kerman, Hamadan, and Tehran. Genomic DNA extraction was performed by a modified salting out method. The polymerase chain reaction amplification conditions were also separately undertaken for each marker. All microsatellite loci revealed polymorphisms in the studied population. Genetic variation was examined using 12 microsatellite loci (HMS7, HMS3, HMS2, HMS6, ASB2, ASB23, VHL20, HTG10, LEX33, ASB17, AHT4, and AHT5). We found that the means of the observed and effective number of alleles were 7.58 and 4.95, with the minimum and maximum values for each of these indices associated with the loci of HMS2 and ASB17, respectively. Moreover, the mean of observed and expected heterozygosity, polymorphism information content, and Shannon's Information Index of the Iranian Kurdish population were 0.77, 0.78, 0.75, and 1.67, respectively, indicating a high degree of genetic diversity in the entire studied population. More specifically, we acquired a range of new alleles in the Iranian Kurdish horse breed that differed in their genetic structure to those of other Iranian breeds in other studies. This study provides an exciting opportunity to improve our knowledge of genetic information which will be beneficial as a base to identify purebred Kurdish horses for a further Iranian Kurdish horse genetic and breeding program.
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http://dx.doi.org/10.1016/j.jevs.2020.103358DOI Listing
March 2021

Putrescine-functionalized carbon quantum dot (put-CQD) nanoparticles effectively prime grapevine (Vitis vinifera cv. 'Sultana') against salt stress.

BMC Plant Biol 2021 Feb 27;21(1):120. Epub 2021 Feb 27.

Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Limassol, Cyprus.

Background: Salinity is an important global problem with destructive impacts on plants leading to different biochemical and metabolic changes in plants through induced oxidative stress that disturbs metabolism, growth, performance and productivity of plants. Given that putrescine (Put) and carbon quantum dots (CQDs), individually, have promising effects in different plant processes, the idea of their combination in a nano-structure "Put-CQD" lead to its synthesis to evaluate the potential exertion of synergistic effects. The current study aimed to investigate the application of newly-synthesized nanoparticles (NPs) consisting of CQDs and Put in grapevine (Vitis vinifera cv. 'Sultana') under salinity stress conditions. For this purpose, Put, CQDs and Put-CQD NPs at 5 and 10 mg L concentrations were applied as chemical priming agents in 'Sultana' grapevine 48 h prior salinity stress imposition (0 and 100 mM NaCl).

Results: Salinity significantly decreased (P ≤ 0.05) morphological parameters, photosynthetic pigments, chlorophyll fluorescence parameters and membrane stability index. In addition, salinity enhanced MDA, HO, proline content and antioxidant enzyme activity. Results revealed that Put-CQD NPs, particularly at 10 mg L concentration, alleviated the destructive impacts of salinity stress by improving leaf fresh and dry weights, K content, photosynthetic pigments, chlorophyll fluorescence and SPAD parameters, proline content, total phenolics and antioxidant enzymatic activities (CAT, APX, GP and SOD), while decreasing Na content, EL, MDA and HO levels.

Conclusion: To conclude, Put-CQD NPs represent an innovative priming treatment that could be effectively applied on grapevine to improve plant performance under salinity stress conditions.
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http://dx.doi.org/10.1186/s12870-021-02901-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7913407PMC
February 2021

Effect of Diode Low-level Laser Irradiation Time on Socket Healing.

J Contemp Dent Pract 2020 Jun 1;21(6):640-644. Epub 2020 Jun 1.

Rafsanjan University of Medical Sciences, Rafsanajan, Iran, Phone: +98 9157648401, e-mail:

Aim: The purpose of this research was to survey the effect of low-level laser irradiation time on socket healing in rats.

Materials And Methods: This randomized nonblinded animal study was done on 24 male rats that were divided into four groups. First maxillary molars of rats were extracted through general anesthesia, and laser was used in all four groups: first group with zero radiation time, second group with 3 minutes, third group with 5 minutes, and fourth group with 10 minutes of radiation by the diode laser (power: 100 mW, wavelength: 980 nm). Half of the rats (three rats) were sacrificed on the 3rd day and another half of rats were sacrificed on the 7th day. Then, the presence of angiogenesis, bone trabeculae, fibroblasts, neutrophil cells, macrophage cells, and lymphocyte cells was assessed. Data were analyzed by SPSS (version 21) using parametric tests.

Results: Among 24 rats, on the 3rd day, the percentage of macrophage and bone trabecula increased significantly in the 5 minute group ( = 0.041 and < 0.01, respectively). Other changes in days 3 and 7 were not statistically significant ( > 0.05).

Conclusion: Low-level laser radiation can accelerate the process of tooth socket healing, which was particularly noticeable in the 5 minute radiation over 3 days.

Clinical Significance: Using a low-level laser can be helpful in accelerating the healing of the tooth socket and reduce the complications after tooth extraction.
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June 2020

Effects of Prehospital Traige and Diagnosis of ST Segment Elevation Myocardial Infarction on Mortality Rate.

Int J Gen Med 2020 4;13:569-575. Epub 2020 Sep 4.

Department of Anesthesiology, School of Medicine, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Introduction: Adverse outcomes and mortality associated with STEMI (ST segment elevation myocardial infarction) are associated with the management and diagnosis time. The aim of this study is to evaluate the outcomes of prehospital diagnosis of STEMI via emergency medical service (EMS) on mortality, in comparison to the patients who did not receive EMS.

Methods: This retrospective study included STEMI patients, who underwent primary angioplasty. The patients were categorized as group A: referred without emergency service, group B: patients who did not receive PPCI and group C: patients referred via ambulance and received telecardiology. Medical records of these patients were evaluated for the diagnosis time, door-to-balloon time, in-hospital, six months, one year and three-year mortality, left ventricular ejection fraction and previous history of cardiovascular conditions and surgeries. The data were recorded and statistically analyzed using SPSS v21.

Results: Of 424 patients studied, 79 were referred without emergency service (group A), 52 patients did not receive PPCI (group B) and 293 patients were referred via ambulance with telecardiology (group C). Door-to-balloon time was least in group C (57.78 min) compared to group A (141.70 min). In-hospital, six months, one year and three-year mortality was least in group C, however, the difference was not statistically significant. The left ventricular ejection fraction was significantly greater in group C.

Conclusion: The results of our study indicate that prehospital diagnosis and telecardiology significantly reduce door-to-balloon time in STEMI patients referred for percutaneous intervention and might have an influence on short-term and long-term mortality rates.
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http://dx.doi.org/10.2147/IJGM.S260828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481285PMC
September 2020

The Efficacy of Ketamine Administration in Prehospital Pain Management of Trauma Patients; a Systematic Review and Meta-Analysis.

Arch Acad Emerg Med 2020 30;8(1):e1. Epub 2019 Oct 30.

Department of Emergency Medicine, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Introduction: Although previous articles and reviews suggest that ketamine might effectively manage pain in trauma patients, these articles have serious limitations. Accordingly, the current meta-analysis aims to investigate the efficacy of ketamine administration in prehospital pain management of trauma patients.

Method: In the present meta-analysis, controlled human studies were included. An extensive search was conducted in electronic databases including Medline (via PubMed), Embase, Central, Scopus, Web of Science, and ProQuest, gathering data to the end of 2018. The efficacy and side effects of ketamine administration in pre-hospital pain management were compared with those of opioid analgesics based on standard mean difference (SMD) and odds ratio (OR) calculations with 95% confidence interval (95% CI).

Results: Data from seven articles were included in the present meta-analysis. Ketamine administration was not more effective than administrating morphine or fentanyl in prehospital pain management of trauma patients (SMD = -0.56, 95% CI: -1.38 to 0.26, p = 0.117). However, co-administration of ketamine+morphine was considerably more effective than ketamine alone, in alleviating pain in prehospital settings (SMD = -0.62, 95% CI: -1.12 to -0.12, p = 0.010). Finally, it was concluded that ketamine alone had less side effects than morphine alone (OR = 0.25, 95% CI: 0.11 to 0.56, p = 0.001). However, co-administration of ketamine+morphine increases the risk of side effects to 3.68 times compared to when morphine is prescribed solely (OR=3.68, 95% CI: 1.99 to 6.82, p<0.001).

Conclusion: For the first time, findings of the current meta-analysis demonstrated that ketamine, being administered alone, is an effective and safe medication in prehospital pain management in trauma patients, and can be considered as an acceptable alternative to opioid analgesics.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6946038PMC
October 2019

Beneficial effects of dietary coenzyme Q10 on the productive and reproductive variables of broiler breeder hens.

Anim Reprod Sci 2020 Feb 12;213:106256. Epub 2019 Dec 12.

Department of Poultry Science, Mississippi State University, MS 39762, USA.

The aim of this study was to evaluate the effects of supplementary CoQ10 in the diets of aged broiler breeder hens on productive and reproductive variables. A total of 128 hens)44 weeks of age) were randomly assigned to one of 16 groups (eight hens per group). The hen-groups (with equal mean egg production and egg weight) were randomly assigned to one of four diet-groups to provide four pen/groups per treatment. There was no CoQ10 supplementation or supplemental amounts of either 300, 600 or 900 mg CoQ10/kg added to the basal diet. Egg production, weight, and mass were determined weekly. To assess fertility, hatchability, and sperm penetration (SP) rate, the hens were artificially inseminated on a weekly basis (from 47-54 weeks of age). The hens were weighed and killed at the end of the experiment for evaluation of the ovarian morphology, oviduct histology, utero-vaginal junction (UVJ) total antioxidant capacity (TAC), and Pdss2, GDF9, and BMP15 mRNA transcript abundances in the germinal disc regions. The results indicated that there was a linear response curve to increasing amounts of supplemental dietary CoQ10 on fertility, hatchability of eggs, SP rates, TAC of the UVJ, fold height and surface epithelia of the magnum and isthmus, and abundance of GDF9, BMP15 and Pdss2 mRNA transcripts in the germinal disc region. In conclusion, the findings of the present study indicate diet supplementation with CoQ10 had beneficial effects on the productive and reproductive variables of aged hens.
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http://dx.doi.org/10.1016/j.anireprosci.2019.106256DOI Listing
February 2020

Pre-hospital pain management; a systematic review of proposed guidelines.

Arch Acad Emerg Med 2019 6;7(1):e55. Epub 2019 Oct 6.

Prehospital and Hospital Emergency Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Introduction: A standard guideline concerning pre-hospital pain management is still a matter of discussion. Therefore, the current umbrella review is determined to perform a comprehensive search in databases and Grey literature and collect and summarize the guidelines and protocols dealing with prehospital pain management.

Methods: In the present study, all of the available guidelines and protocols concerning pre-hospital pain management were reviewed. Presented guidelines are from 2010 up to present, as the majority of guidelines are considered old and become renewed after 10 years. Finally, the development quality of each guideline was evaluated using AGREE II instrument.

Results: The search conducted in databases and non-indexed protocols resulted in inclusion of 12 pre-hospital pain management guidelines. The time interval of the guidelines was from 2010 to 2019. Four guidelines were designed for pain management in trauma patients and other guidelines were presented for all of the clinical conditions associated with pain. All of the 12 included guidelines presented pain management instructions in adults. Pain management in children was reported in 10 guidelines. All of the guidelines persisted on a standard method for pain evaluation. Pain management was categorized in three groups; mild, moderate and severe pain. Most of the guidelines recommend paracetamol as an optional treatment for management of mild pain in both adults and children. In management of moderate and severe pain, fentanyl and morphine were suggested for both adults and children. In most of the treatment guidelines fentanyl is the optional choice for children.

Conclusion: The present umbrella review has summarized the current evidence in pre-hospital pain management for the first time via investigation of guidelines and protocols related to the matter. Based on the obtained evidence, no guideline is yet presented concerning opioid-free management of moderate and severe pain. The evidence is insufficient for using non opioid medications such as ketamine.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6905420PMC
October 2019

Use of supplemental dietary coenzyme Q10 to improve testicular function and fertilization capacity in aged broiler breeder roosters.

Theriogenology 2020 Jan 14;142:355-362. Epub 2019 Oct 14.

Department of Poultry Science, Mississippi State University, MS, 39762, USA.

In numerous studies it has been suggested that targeting mitochondria with specific compounds could efficiently inhibit various conditions associated with oxidative stress. The treatment of aged roosters with compounds such as coenzyme Q10 (CoQ10), may improve their reproductive performance by providing protection from oxidative stress. Therefore, this study was performed to assess the effect of supplemental dietary CoQ10 on the testicular function and fertility of aged broiler breeder roosters. A total of 36 roosters)47 weeks of age) were randomly divided into dietary treatments containing either 0, 300 or 600 mg CoQ10/kg diet. Three birds were allocated to each of four replicate groups in each dietary treatment. Between 47 and 54 weeks of age, ejaculates were obtained weekly from the three roosters in each replicate group. Samples in a replicate were pooled and analyzed as a single sample. Between 51 and 54 weeks of age, seminal plasma total antioxidant capacity (TAC), alanine amino transferase (ALAT) and aspartate amino transferase (ASAT) levels were assessed. Fertility, hatchability, and sperm penetration (SP) rates were likewise evaluated. Seminal volume, sperm concentration, sperm plasma membrane functionality, sperm plasma membrane integrity, seminiferous tubule diameter and seminiferous epithelium thickness exhibited quadratic increases in response to increasing levels of dietary CoQ10. Respectively, the 429.19, 433.33, 464.50, 613.50, 392.78 and 447.99 mg/kg dietary concentrations of CoQ10 provided the best results for each of the aforementioned variables. Also, other seminal traits, as well as testosterone concentration, fertility, and SP rates, displayed linear increases in response to the increasing levels of CoQ10. Dietary supplementation of CoQ10 linearly decreased seminal plasma ALAT and ASAT and linearly increased seminal plasma TAC. In conclusion, CoQ10 supplementation in the diet (a minimum of 300 mg CoQ10/kg diet) has the potential to improve the reproductive performance of aged broiler breeder roosters.
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http://dx.doi.org/10.1016/j.theriogenology.2019.10.011DOI Listing
January 2020

Exploring novel single nucleotide polymorphisms and haplotypes of the diacylglycerol O-acyltransferase 1 (DGAT1) gene and their effects on protein structure in Iranian buffalo.

Genes Genomics 2019 11 6;41(11):1265-1271. Epub 2019 Aug 6.

Department of Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Joongang-Ro 327, Ansung, Kyeonggi-Do, 456-749, Korea.

Background: Diacylglycerol O-acyltransferase 1 (DGAT1) plays a key role in the synthesis of triglycerides. Recent studies have shown that a transition mutation resulting in substitutions of guanine by adenine in the DGAT1 gene in cattle has considerable effects on milk yield and composition. Currently, there is no systematic research reporting on the utilization of this gene segment in Iranian buffalo (Bubalus bubalis).

Objective: In this study, the genetic differentiation of three indigenous Iranian buffalo populations was investigated in the region spanning exon 3 to exon 17 of the DGAT1 gene.

Methods: A total of 200 buffaloes were genotyped, all the samples were sequenced directly in both directions with forward and reverse sequencing primers.

Results: Sequence analysis showed novel SNPs compared to the reference GenBank sequence (DQ886485) at nucleotide positions g.6097A>G, g.7036C>T, g.7338G>A, g.7710C>T, g.8087C>T, g.8259G>A, g.8275G>A, g.8367C>T, and g.8426C>T. No polymorphisms were found within exon 8. Therefore, the K232A position was thought to be a conserved and fixed region for high milk fat content (K allele) in Bos indicus and all buffalo breeds. Comparison with Indian buffalo revealed three exonic SNPs, one of which was nonsynonymous. A unique 22 bp insertion was observed in intron 10 of DGAT1. Linkage disequilibrium analysis allowed the identification of nine haplotypes among the sampled animals. To our knowledge, this is the first report of sequencing analysis of the DGAT1 gene in Iranian buffalo.

Conclusion: Our results suggest that genetic diversity exists and could be useful in examining the association between the DGAT1 gene and milk production traits in buffalo.
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http://dx.doi.org/10.1007/s13258-019-00854-2DOI Listing
November 2019

Study of whole genome linkage disequilibrium patterns of Iranian water buffalo breeds using the Axiom Buffalo Genotyping 90K Array.

PLoS One 2019 31;14(5):e0217687. Epub 2019 May 31.

Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia, Australia.

Accuracy of genome-wide association studies, and the successful implementation of genomic selection depends on the level of linkage disequilibrium (LD) across the genome and also the persistence of LD phase between populations. In the present study LD between adjacent SNPs and LD decay between SNPs was calculated in three Iranian water buffalo populations. Persistence of LD phase was evaluated across these populations and effective population size (Ne) was estimated from corrected r2 information. A set of 404 individuals from three Iranian buffalo populations were genotyped with the Axiom Buffalo Genotyping 90K Array. Average r2 and |D'| between adjacent SNP pairs across all chromosomes was 0.27 and 0.66 for AZI, 0.29 and 0.68 for KHU, and 0.32 and 0.72 for MAZ. The LD between the SNPs decreased with increasing physical distance from 100Kb to 1Mb between markers, from 0.234 to 0.018 for AZI, 0.254 to 0.034 for KHU, and 0.297 to 0.119 for MAZ, respectively. These results indicate that a density of 90K SNP is sufficient for genomic analyses relying on long range LD (e.g. GWAS and genomic selection). The persistence of LD phase decreased with increasing marker distances across all the populations, but remained above 0.8 for AZI and KHU for marker distances up to 100Kb. For multi-breed genomic evaluation, the 90K SNP panel is suitable for AZI and KHU buffalo breeds. Estimated effective population sizes for AZI, KHU and MAZ were 477, 212 and 32, respectively, for recent generations. The estimated effective population sizes indicate that the MAZ is at risk and requires careful management.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0217687PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544294PMC
January 2020

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology.

Reprod Fertil Dev 2018 Jun;30(7):1038-1048

Department of Poultry Science, University of Georgia, Athens, GA 30602-2772, USA.

Male broiler breeders (n=32) of 55 weeks of age were administered four different doses of capsulated d-aspartate (DA; 0, 100, 200 or 300mgkg-1day-1, p.o. (DA0, DA100, DA200 and DA300 respectively)) for 12 successive weeks to assess reproductive performance, blood testosterone, testicular histology and transcript levels of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc), androgen receptor (AR), LH receptor (LHR), 3β-hydroxysteroid dehydrogenase (3BHSD), proliferating cell nuclear antigen (PCNA), glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) and glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B). Blood samples and ejaculates were collected, and bodyweight was recorded weekly for 10 weeks. AI was performed weekly for the last 2 weeks to determine the number of sperm penetration holes in the perivitelline layer, fertility and hatchability. Testes histology and transcript levels were evaluated in the 12th week. Bodyweight, numbers of Leydig cells and blood vessels, testis index and levels of sperm abnormalities were not affected (P>0.05) by the treatment. However, sperm total and forward motility, plasma membrane integrity and functionality of sperm, ejaculate volume, testosterone concentration and fertility were higher (P<0.05) in both the DA200 and DA300 groups compared with the other groups. In the DA100 and DA200 groups, sperm concentration, number of spermatogonia, thickness of the seminiferous epithelium and the diameter of tubules were significantly higher (P<0.05) than the other DA-treated groups. The number of penetration holes, hatchability and malondialdehyde concentration were higher in the DA200, all DA-treated and DA300 groups respectively compared with the control and other treatment groups. Except for P450scc, AR, LHR and PCNA transcript levels in the DA300 groups, the relative expression of the genes evaluated improved significantly in the other DA-treated groups. Based on these experimental findings, it is concluded that DA improves reproductive performance of aged roosters.
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http://dx.doi.org/10.1071/RD17072DOI Listing
June 2018

Dietary Guanidinoacetic acid modulates testicular histology and expression of c-Kit and STRA8 genes in roosters.

Theriogenology 2019 May 12;130:140-145. Epub 2019 Mar 12.

Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Decline in semen quality is considered as a major contributing factor in age-related subfertility of broiler breeder flocks. This study was aimed to investigate the effect of dietary supplementation of Guanidinoacetic acid (GAA), as an alternative energy source along with antioxidant potential, on testicular histology and relative gene expression of some spermatogonial markers (c-Kit and STRA8) in aged roosters. Sixteen 24-week-old male broiler breeders were randomly allocated into four groups and fed a basal diet supplemented with increasing levels of GAA including 0 (GAA-0), 600 (GAA-600), 1200 (GAA-1200) or 1800 (GAA-1800) mg/kg diet/day for 26 successive weeks. At the end of the experiment, all the birds were killed and two ipsilateral testicle samples were taken to either quantify relative gene expression or do histology. Except for seminiferous tubules' diameter, testicular weight, and the number of blood vessels, dietary supplementation of GGA improved the epithelium thickness of seminiferous tubules, the number of spermatogonia and Leydig cells and the relative gene expression of c-Kit and STRA8 (P < 0.01). Increasing levels of GAA cubically affected (P < 0.01) the diameter of seminiferous tubules and their epithelium thickness as well as the number of spermatogonia. However, number of Leydig cells and relative expression of c-Kit were linearly, and relative expression of STRA8 was quadratically (P < 0.01) enhanced in response to graded levels of GAA supplementation. Taking all parameters into account, daily supplementation of 1300-1450 mg of GAA/kg diet was estimated as an optimum dosage maximizing the evaluated traits.
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http://dx.doi.org/10.1016/j.theriogenology.2019.03.006DOI Listing
May 2019

The effect of coenzyme Q10 on rooster semen preservation in cooling condition.

Theriogenology 2019 Apr 26;129:103-109. Epub 2019 Feb 26.

Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Oxidative stress has been known as a significant cause of the lower fertility rates correlated with liquid stored rooster semen. The effect of coenzyme Q10 (CoQ10), as a powerful antioxidant, seems be beneficial on semen storage of broiler breeder roosters at the cooled condition. Therefore, two experiments were performed to assess the effect of CoQ10 supplemented semen extender on sperm quality parameters, fertility, hatchability and sperm penetration (SP) rates of rooster semen stored at 5 °C. In the first experiment, semen samples of 12 roosters were weekly pooled for four weeks (47-50 weeks of age). The pooled semen was diluted by modified Beltsville poultry semen extender and divided into three equivalent parts containing different levels of CoQ10 [0 (Q-0), 100 (Q-100) and 200 (Q-200) μM/mL) and then stored for 24 h at 5 °C. Sperm quality including progressive motility, plasma membrane integrity and functionality were evaluated after 0 and 24 h storage. The results showed that progressive motility, plasma membrane integrity and functionality were improved in Q-200 compared to Q-0 after 24 h storage at 5 °C (P < 0.01, P < 0.05 and P < 0.01, respectively). According to the results of the first experiment, Q-200 group was selected to be used to evaluate the fertility, hatchability and SP rate in the second experiment during next four weeks (51-54 weeks of age). The results of the second experiment showed that fertility rate was significantly increased in Q-200 compared to control group by approximately 10%, although no significant difference was observed in hatchability and SP rates between Q-200 and control groups. In conclusion, the results of the present research confirm that supplementation of rooster semen extender with CoQ10 might be potentially used to improve semen quality and fertility rates.
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http://dx.doi.org/10.1016/j.theriogenology.2019.02.028DOI Listing
April 2019

Expression of genes related to liver fatty acid metabolism in fat-tailed and thin-tailed lambs during negative and positive energy balances.

J Anim Physiol Anim Nutr (Berl) 2019 Mar 14;103(2):427-435. Epub 2018 Dec 14.

Departmen of Animal Science, University of Tehran, Karaj, Iran.

Fat-tailed sheep breeds can tolerate periods of negative energy balance without suffering from elevated concentration of plasma non-esterified fatty acid (NEFA). This ability was attributed to unique metabolism of fat-tailed adipose depot, whereas role of liver as an influential organ in fatty acid metabolism was not evaluated yet. Hence, current study was conducted to evaluate the effects of negative and positive energy balances on liver expression of genes related to fatty acid metabolism in fat-tailed and thin-tailed lambs. Lambs experienced negative (21 days) and positive (21 days) energy balances and were slaughtered at the beginning and end of negative energy balance and at the end of positive energy balance. Real-time quantitative polymerase chain reaction (RT-Q-PCR) was conducted to evaluate changes in gene expression. Expression of diglyceride acyltransferase 1 (DGAT1), 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) and apolipoprotein B (APOB) was not affected by genotype, energy balance and their interaction. Expression of carnitine palmitoyltransferase 1 (CPT1) was significantly higher in liver of fat-tailed comparing to thin-tailed lambs regardless of energy balance (p < 0.02). Catalase mRNA abundance was increased in response to negative energy balance (p < 0.02), and severity of this enhancement was higher in fat-tailed lambs (p < 0.06). Expression of CPT1 was positively correlated with expression of HMGCS2 in both fat-tailed (p < 0.05) and thin-tailed lambs (p < 0.002); however, the correlation was weaker in fat-tailed lambs (0.72 vs. 0.57, respectively, for thin-tailed and fat-tailed lambs). There was a positive correlation between DGAT1 and APOB genes expression in fat-tailed lambs (0.94; p < 0.001), whereas this correlation was not observed in thin-tailed lambs. Results demonstrate that liver of fat-tailed lambs has higher capacity for metabolism of mobilized NEFA exposed to liver during negative energy balance.
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http://dx.doi.org/10.1111/jpn.13036DOI Listing
March 2019

A genome-wide scan for signatures of selection in Azeri and Khuzestani buffalo breeds.

BMC Genomics 2018 Jun 11;19(1):449. Epub 2018 Jun 11.

Davies Research Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, SA, 5371, Australia.

Background: Identification of genomic regions that have been targets of selection may shed light on the genetic history of livestock populations and help to identify variation controlling commercially important phenotypes. The Azeri and Kuzestani buffalos are the most common indigenous Iranian breeds which have been subjected to divergent selection and are well adapted to completely different regions. Examining the genetic structure of these populations may identify genomic regions associated with adaptation to the different environments and production goals.

Results: A set of 385 water buffalo samples from Azeri (N = 262) and Khuzestani (N = 123) breeds were genotyped using the Axiom® Buffalo Genotyping 90 K Array. The unbiased fixation index method (F) was used to detect signatures of selection. In total, 13 regions with outlier F values (0.1%) were identified. Annotation of these regions using the UMD3.1 Bos taurus Genome Assembly was performed to find putative candidate genes and QTLs within the selected regions. Putative candidate genes identified include FBXO9, NDFIP1, ACTR3, ARHGAP26, SERPINF2, BOLA-DRB3, BOLA-DQB, CLN8, and MYOM2.

Conclusions: Candidate genes identified in regions potentially under selection were associated with physiological pathways including milk production, cytoskeleton organization, growth, metabolic function, apoptosis and domestication-related changes include immune and nervous system development. The QTL identified are involved in economically important traits in buffalo related to milk composition, udder structure, somatic cell count, meat quality, and carcass and body weight.
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http://dx.doi.org/10.1186/s12864-018-4759-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996463PMC
June 2018

Dynamic modeling of folliculogenesis signaling pathways in the presence of miRNAs expression.

J Ovarian Res 2017 Dec 19;10(1):76. Epub 2017 Dec 19.

Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.

Background: TEK signaling plays a very important role in folliculogenesis. It activates Ras/ERK/MYC, PI3K/AKT/mTORC1 and ovarian steroidogenesis activation pathways. These are the main pathways for cell growth, differentiation, migration, adhesion, proliferation, survival and protein synthesis.

Results: TEK signaling on each of the two important pathways where levels of pERK, pMYC, pAkt, pMCL1 and pEIF4EBP1 are increased in dominant follicles and pMYC is decreased in dominant follicles. Over activation of ERK and MYC which are the main cell growth and proliferation and over activation of Akt, MCl1, mTORC1 and EIF4EBP1 which are the main cell survival and protein synthesis factors act as promoting factors for folliculogenesis. In case of over expression of hsa-miR-30d-3p and hsa-miR-451a, MYC activity level is considerably increased in subordinate follicles. Our simulation results show that in the presence of has-miR-548v and bta-miR-22-3p, downstream factors of pathways are inhibited.

Conclusions: Our work offers insight into the design of natural biological procedures and makes predictions that can guide further experimental studies on folliculogenesis pathways. Moreover, it defines a simple signal processing unit that may be useful for engineering synthetic biology and genes circuits to carry out cell-based computation.
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http://dx.doi.org/10.1186/s13048-017-0371-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735818PMC
December 2017

Psychometric Evaluation of the Persian Version of Barkley Adult Attention Deficit/Hyperactivity Disorder Screening Tool among the Elderly.

Scientifica (Cairo) 2017 1;2017:9109783. Epub 2017 Nov 1.

Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

Background: The Barkley Adult Attention Deficit/Hyperactivity Disorder (ADHD) Rating Scale-IV (BAARS-IV) was developed, and it demonstrated good psychometric properties. The BAARS-IV includes 27 questions on the symptoms of adult ADHD. The purpose of the present study is to investigate the psychometric testing of the Persian version of BAARS-IV among the elderlies in Tabriz City.

Method: This cross-sectional study was conducted in Tabriz City-in the west of Iran-in 2015 via enrolling of 121 old-aged people. We did the process of translation and adaptation of BAARS-IV and examined its concurrent validity, internal consistency, and test-retest reliability.

Result: The BAARS-IV demonstrated good internal consistency and test-retest reliability. Correlations between the BAARS-IV and the CAARS-S: SV were high and evidence supporting concurrent validity was revealed. Cronbach's alpha for the overall scale and subscales stood at 0.89, 0.81, 0.66, 0.56, and 0.82, respectively.

Conclusion: The Persian BAARS-IV showed acceptable reliability and validity. BAARS-IV was determined to be composed of internally consistent and psychometrically sound items.
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http://dx.doi.org/10.1155/2017/9109783DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687135PMC
November 2017

Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production.

Theriogenology 2018 Jan 16;106:265-270. Epub 2017 Oct 16.

Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran; Department of Animal Science, Ramin Agriculture and Natural Resource University of Khozestan, Ahvaz, Iran.

Investigations in the past decades have shown that oocytes developmental competence following in vitro fertilization is greatly influenced by an interval between isolation of the ovaries immediately after death/slaughter and oocytes recovery from the visible follicles. In order to determine the optimal conditions for long-term preservation of ovaries, an experiment was conducted with adding different doses of melatonin (0 (C), 500 (M1), 600 (M2), 700 (M3) and 800 (M4) μM) as an antioxidant to sheep ovaries preservation medium (PBS) maintained at 4 and 20 °C for 24 h. The effects on in vitro embryo production (IVEP) parameters including maturation, fertilization, cleavage, and blastocyst rates and the total number of blastomere were evaluated after the ovaries preservation. Melatonin reduced the decline in fertilization rate as an indicator of success in vitro maturation (P ≤ 0.05). Furthermore, ovarian storage time had significant negative effect (P ≤ 0.05) on IVEP parameters. Supplementation with melatonin increased the total cell number of blastocysts as an indicator of embryo quality (i.e. mean blastomeric cells in 4°C groups: 86.00 ± 3.00, 98.50 ± 3.5, 111.5 ± 1.5, 125.5 ± 2.00 and 126.50 ± 5.5 for C, M1, M2, M3 and M4. respectively). Overall, the results showed that the use of melatonin antioxidant in the ovaries storage medium had beneficial effects on sheep oocytes development and embryos quality.
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http://dx.doi.org/10.1016/j.theriogenology.2017.10.009DOI Listing
January 2018

Regulation of lipid metabolism in adipose depots of fat-tailed and thin-tailed lambs during negative and positive energy balances.

Gene 2018 Jan 21;641:203-211. Epub 2017 Oct 21.

Department of Animal Science, University of Tehran, P.O. Box # 3158711167-4111, Karaj, Iran.

This study aimed to evaluate the effects of negative and positive energy balances on gene expression of regulators and enzymes controlling lipogenesis and lipolysis in muscle and adipose depots of fat-tailed and thin-tailed lambs. Lambs were slaughtered during neutral, negative and positive energy balances for sample collection. Real time q-PCR was conducted to measure the gene expression. Expression of PPARγ was increased in response to positive energy balance regardless of genotype and type of tissue (P<0.04). Expression of SREBF1 was reduced in response to negative and positive energy balances in fat-tailed lambs, whereas in thin-tailed lambs, downregulated SREBF1 was restored during positive energy balance (P<0.01). Enhancement in FABP4 expression in response to negative and positive energy balances was respectively higher in thin-tailed and fat-tailed lambs affected by interaction of genotype and energy balance (P<0.11). In thin-tailed lambs, the enhanced FABP4 expression in response to negative energy balance was considerably higher in mesenteric adipose depot, whereas in fat-tailed lambs, positive energy balance induced enhancement in FABP4 expression was considerably higher in fat-tail adipose depot. The results demonstrate that transcription regulation of lipogenesis and lipolysis during negative and positive energy balances occurs differently in fat-tailed and thin-tailed lambs. Thin-tailed and fat-tailed lambs are respectively more responsive to negative and positive energy balances and mesenteric and fat-tail adipose depots respectively in thin-tailed and fat-tailed lambs are the main adipose depots responsible for higher responsiveness of thin-tailed and fat-tailed lambs to negative and positive energy balances.
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http://dx.doi.org/10.1016/j.gene.2017.10.065DOI Listing
January 2018

miRNA-mRNA network involved in folliculogenesis interactome: systems biology approach.

Reproduction 2017 07 27;154(1):51-65. Epub 2017 Apr 27.

Molecular Biology Research CenterBaqiyatallah University of Medical Sciences, Tehran, Iran

At later phases of folliculogenesis, the mammalian ovarian follicle contains layers of granulosa cells surrounding an antral cavity. To better understand the molecular basis of follicular growth and granulosa cell maturation, we study transcriptome profiling of granulosa cells from small (<5 mm) and large (>10 mm) bovine follicles using simultaneous method of Affymetrix microarrays (24,128 probe sets) and RNA-Seq data sets. This study proposes a computational method to discover the functional miRNA-mRNA regulatory modules, that is, groups of miRNAs and their target mRNAs that are believed to take part cooperatively in post-transcriptional gene regulation under specific conditions. The reconstructed network was named Integrated miRNA-mRNA Bipartite Network. 277 genes and 6 key modules were disclosed through clustering for mRNA master list. The 66 genes are among the genes that belong to at least two modules. All these genes, being involved in at least one of the phenomena, namely cell survival, proliferation, metastasis and apoptosis, have an overexpression pattern ( < 0.01). For miRNA master list, a total of 172 sequences were differentially expressed ( < 0.01) between dominant (large) and each of subordinate (small) follicles. Within the follicle, these miRNAs were predominantly expressed in mural granulosa cells. Finally, predicted and validated targets of these miRNAs enriched in dominant (large) follicles were identified, which are mapped to signaling pathways involved in follicular cell proliferation, steroidogenesis, PI3K/AKT/mTOR and Ras/Raf/MEK/ERK. The identification of miRNAs and their target mRNAs and the construction of their regulatory networks may give new insights into biological procedures.
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http://dx.doi.org/10.1530/REP-17-0049DOI Listing
July 2017

Improvement of post-thawed sperm quality and fertility of Arian rooster by oral administration of d-aspartic acid.

Theriogenology 2017 Apr 8;92:69-74. Epub 2017 Jan 8.

Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran; Department of Embryology at Reproduction Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran.

This study was conducted to investigate the effect of d-Aspartic acid (D-Asp) on post-thawed sperm quality, fertility and hatchability outcomes in male broiler breeders. Twenty 55-week-old roosters were selected and equally split into four groups (n = 5 rooster/group). Different daily D-Asp doses including 0 (D-0), 100 (D-100), 200 (D-200) or 300 (D-300) mg/kg BW were capsulated and individually administered for 12 weeks to roosters in each group. Semen samples were weekly collected from 7th to 12th week of experiment. Sperm quality from 7th to 11th week was evaluated in both fresh (total and forward motility and plasma membrane functionality) and post-thawed (total and forward motility, plasma membrane functionality, apoptosis status and mitochondrial activity) conditions. Also, collected semen samples on the 12th week were frozen and artificially inseminated to evaluate fertility and hatchability. The results from fresh condition showed that total and forward motility and plasma membrane functionality were significantly higher in D-200 compared to other groups. Also, interaction effect of time and treatment was not significant for all assessed parameters in fresh condition. In post-thawed condition, D-200 showed significantly higher total and forward motility, fertility and hatchability compared to other groups. The higher value for plasma membrane functionality and mitochondrial activity was observed in D-200 compared to D-0 and D300 groups. However, the percentage of live, early apoptotic and dead spermatozoa were not significantly affected by applied treatment in the current study. No significant difference for time and treat interaction effect was observed for all assessed parameters except forward motility. In conclusion, it seems that D-Asp administration could improve fresh and post-thawed sperm quality and post-thawed sperm fertility in male broiler breeders.
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http://dx.doi.org/10.1016/j.theriogenology.2017.01.014DOI Listing
April 2017

Incidence and Mortality of Testicular Cancer and Relationships with Development in Asia.

Asian Pac J Cancer Prev 2016 ;17(9):4251-4257

Faculty of Health, Department of Biostatistics and Epidemiology, Tabriz University of Medical Science, Tabriz, Iran E-mail :

Background: Testicular cancer is one of the most common cancers among young men between ages 20-34 in countries with high or very high levels of the Human Development Index (HDI). This study investigated the incidence and mortality of prostate cancer and the relationship with the HDI and its dimensions in Asia in 2012.

Materials And Methods: The study was conducted based on data from the world data of cancer and the World Bank (including the HDI and its components). Standardized incidence and mortality rates of testicular cancer were calculated for Asian countries. Correlations between incidence and/ormortality rates, and the HDI and its components were assessed with the use of the correlation test, using SPSS software.

Results: There was a total of 14902 incidences and 5832 death were recorded in Asian countries in 2012. Among the Asian countries, the five countries with the highest standardized incidence rates of testicular cancer were Israel, Georgia, Turkey, Lebanon and Kazakhstan and the five countries with the highest standardized mortality rates were Turkey, Georgia, Jordan, Cambodia and the Syrian Arab Republic. A positive correlation of 0.382 was observed between the standardized incidence rates of testicular cancer and the HDI (p=0.009). Also a negative correlation of 0.298 between the standardized mortality rate of testicular cancer and the Human Development Index was noted although this relation was statistically non-significant (p=0.052).

Conclusions: There is a positive correlation between HDI and the standardized incidence rate of testicular cancer and negative correlation with standardized mortality rate.
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February 2017

Identification of novel SNP in caprine β-lactoglobulin gene.

J Genet 2016 Sep;95(3):485-90

Department of Animal Science University College of Agriculture and Natural Resources, University of Tehran, Karaj 3158711167, Iran.

β-lactoglobulin (β-LG) gene is suggested as a functional candidate gene for milk yield and milk composition. β-LG polymorphism has been reported to be associated with milk yield in cows, sheep and Indian goats. This study was performed to identify SNPs in exon 7 of β-LG gene and their association with milk traits in Iranian local Mahabadi goats using polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) and PCR-sequencing. Three SSCP patterns were observed with frequencies 0.678, 0.096 and 0.226, respectively. Subsequently, after sequencing each unique pattern nine novel mutations were identified. These mutations include: T InDel at nucleotide position 93 and substitutions T/C, T/G, T/C, G/T, T/G,T/C, G/A and A/T at nucleotide positions 99, 124, 126, 134, 147, 156, 176 and 177, respectively. Of these, seven mutations were same among the genotypic patterns while differences were related to T deletion and insertion (-/T) at nucleotide position 93 with frequencies 0.22 and 0.78 in the presence and absence of T allele, respectively; and substitution (A/T) at nucleotide position 177 with frequencies 0.16 and 0.84 for A and T alleles, respectively. Milk traits including milk production (gr), milk fat and protein (%) were also measured. These findings demonstrated that β-LG gene had a significant effect on milk protein percentage (P < 0.05), but had no significant effect on milk production and milk fat percentage.
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http://dx.doi.org/10.1007/s12041-016-0662-xDOI Listing
September 2016
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