Publications by authors named "Morvarid Asadipour"

6 Publications

  • Page 1 of 1

Variations in IL-22, IL-27 and IL-35 serum levels in untreated and treated hepatitis C patients.

Eur Cytokine Netw 2020 Dec;31(4):134-139

Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Hepatitis C virus (HCV) is the leading cause of chronic liver diseases including hepatic fibrosis, cirrhosis, and hepatocellular carcinoma. We aimed to assess serum levels of interleukin (IL)-22, IL-27 and IL-35 in patients with hepatitis C and healthy controls to investigate their possible relationship with viral genotypes and liver enzyme levels.

Method: A total of 30 newly diagnosed hepatitis C patients with no history of antiviral therapy and 30 healthy individuals participated in this study. Serum levels of IL-22, IL-27 and IL-35 were determined by ELISA in peripheral blood samples from patients prior to and following treament with pan-genotypic direct-acting anti-viral therapy. Serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were measured to determine any possible association between hepatic enzymes and cytokine serum levels concentrations.

Result: The results show elevated serum levels of of IL-35 in HCV-infected patients compared to treated cases and healthy controls, whereas there was no significant difference in IL-22 and IL-27 serum levels among the three groups. Additionally, the cytokine levels were not significantly correlated with certain genotypes and levels of liver enzymes.

Conclusion: Our findings indicate a potential role for IL-35 in chronic HCV infection and therapeutic management of patients with hepatitis C infection.
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http://dx.doi.org/10.1684/ecn.2020.0455DOI Listing
December 2020

IL-27, a pleiotropic cytokine for fine-tuning the immune response in cancer.

Int Rev Immunol 2020 Nov 4:1-11. Epub 2020 Nov 4.

Shiraz Institute for Cancer Research, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells. IL-27 binding to IL-27R starts downstream signaling based on the target cells. It can instigate inflammation by inducing CD4 T cell proliferation, Th1 polarization, cytotoxic T cell activation, generation of the natural killer cell, and macrophage and dendritic cell activation. However, by inducing programmed cell death and suppression of effector cells, IL-27 can suppress inflammation and return the immune response to hemostasis. Altogether, IL-27 displays multifaceted dual functions, which may result in either pro- or anti-inflammatory effects. Recent investigations indicated the antitumor activity of IL-27 via inducing Th1, and CTL responses and generating NK cells. On the other hand, IL-27 also can promote tumor cells' proliferation, survival, and angiogenesis. In the present review, we'll discuss recent advances concerning the role of IL-27 in inflammatory diseases such as infections, autoimmune diseases with a focus on cancer.
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http://dx.doi.org/10.1080/08830185.2020.1840565DOI Listing
November 2020

IL-18 in Blood Serum of Hepatitis C Patients Might be of Predictive Value for Individual Outcomes.

Infect Disord Drug Targets 2020 Jul 7. Epub 2020 Jul 7.

Department of Immunology, Shiraz University of Medical Sciences, Shiraz. Iran.

Objective: Pro- inflammatory cytokines including Interleukin (IL)-18 have been shown to be involved in the clearance of Hepatitis C virus (HCV) infection. However, changes in the balance of pro- and anti-inflammatory cytokines production during the immune response, can elicit a variety of liver damage. Therefore, it is of interest to study IL-18 serum levels in hepatitis patients and its correlation with HCV infection.

Methods: Twenty-nine newly diagnosed HCV+ patients with no history of antiviral therapy, and 17 healthy controls were enrolled in our study. Biochemical markers of liver disease were evaluated by biochemistry assay kits. Serum concentrations of IL-18 were determined with the ELISA method before and after treatment with pangenotypic direct-acting antivirals (DAAs) treatment.

Results: Our results showed statistically significant difference in serum levels of IL-18 in HCV+ patients (692.261 ± 48.76) compared to healthy controls (520.00 ± 44.73) (P=0.021). However, there was no significant difference in IL-18 serum levels between treated group compared to untreated patients (P=0.74). No significant correlations were detected between the level of IL-18 and liver enzyme levels.

Conclusion: According to our study, IL-18 might be a disease marker associated with HCV infection; however, this conclusion requires further investigation.
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http://dx.doi.org/10.2174/1871526520666200707113401DOI Listing
July 2020

Anti-leukemic activity of occurs by apoptosis in human cells.

Biotech Histochem 2020 Oct 17;95(7):506-513. Epub 2020 Mar 17.

Immunology Department, Shiraz University of Medical Sciences , Shiraz, Iran.

Failure of apoptosis contributes to leukemia progression. We investigated extracts of a native Iranian plant, , for possible anti-leukemia activity by induction of apoptosis and changes to the cell cycle. Growth inhibition caused by aqueous, butanol, dichloromethane and hexane extracts of on K562 and Jurkat leukemia cells was assessed using a colorimetric assay. Extracts were analyzed for induction of apoptosis and cell cycle arrest using flow cytometry and measurement of caspase-3 activity. Dichloromethane and hexane extracts inhibited leukemia cell proliferation in a dose-dependent manner. The IC values of these extracts were 22-33 µg/ml. Flow cytometric determination of annexinV/propidium iodide positive cells verified a significantly increased percentage of apoptotic cells compared to negative controls. Both 50 μg/ml dichloromethane and hexane extracts induced apoptosis in 89-97% of K562 and 94-97% of Jurkat cells 48 h after treatment. The effects of extracts on the cell cycle included significantly increased numbers of K562 and Jurkat cells in the subG1 phase and decreased numbers of cells in the G1, S and G2/M phases. After 24 h, we found increased levels of caspase-3 activation in cells treated with 25 μg/ml dichloromethane and hexane extracts compared to untreated cells. Our findings indicate the anti-leukemic effects of dichloromethane and hexane extracts of due to induction of apoptosis and inhibition of cell cycle progression.
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http://dx.doi.org/10.1080/10520295.2020.1725834DOI Listing
October 2020

induces cell death and inhibited cell cycle progression in K562 myelogenous and Jurkat T cell leukemia cell lines.

J Immunoassay Immunochem 2019 17;40(5):459-472. Epub 2019 Jun 17.

Immunology Department, Shiraz University of Medical Sciences , Shiraz , Iran.

Several plants of genus have shown anti-tumor activity. We investigated the antileukemia effects of different fractions of (Summer savory). The growth inhibitory effect of fractions on K562 and Jurkat leukemia cells were determined by MTT assay. The most effective fractions were analyzed by flow cytometry and colorimetric assay for apoptosis induction and cell cycle changes. Various fractions from showed growth inhibitory effects on leukemia cells, among them two hexane and dichloromethane fractions with IC values of 32.1-47.8 μg/ml (K562) and 44.3-45.7 μg/ml (Jurkat) were the most effective. According to annexin V staining, both of these fractions significantly induced apoptosis at 50μg/ml in K562 (hexane; 73.06 ± 5.11% and dichloromethane; 96.14 ± 2.33%) and Jurkat cells (hexane; 78.85 ± 11.9% and dichloromethane; 94.05 ± 2.47%) 48 h after treatment. They increased cell accumulation in sub-G1 phase (>50%, < .001) and decreased number of cells in G0-G1, S and G2M phases. The fractions significantly increased the caspase-3 activity in both cell lines (≈2.5-3.5 fold of untreated cells). Hexane and dichloromethane fractions of had the capacity to induce death and change the cell cycle distribution in leukemia cells; therefore they might be good candidates for more studies in regard to their possible therapeutic usefulness in leukemia.
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http://dx.doi.org/10.1080/15321819.2019.1629592DOI Listing
October 2019

The Legacy of Mesenchymal Stem Cells in Vindicating the Clonal Evolution Model of Cancer

Asian Pac J Cancer Prev 2018 Sep 28;19(8):2029-2030. Epub 2018 Sep 28.

Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Email:

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http://dx.doi.org/10.22034/APJCP.2018.19.8.2029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171386PMC
September 2018