Publications by authors named "Mojgan Saki"

2 Publications

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In vitro and Ex vivo Antiparasitic Effect of Rheum ribes L. Extract Against the Hydatid Cyst Protoscoleces.

Infect Disord Drug Targets 2021 ;21(6):e170721187993

Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Cystic echinococcosis is a zoonotic infection in humans and herbivorous animals occurring worldwide, which is caused by the larva stage of Echinococcus granulosus. Rhubarb (Rheum ribes L.) as an herbal medicine has various therapeutic properties such as antioxidant, anticancer, and antimicrobial. With respect to the potential of the biological activities of this plant in traditional and modern medicine, we aim to examine its protoscolicidal effects against E. granulosus protoscoleces in vitro and ex vivo.

Methods: Collected protoscoleces from liver hydatid cysts of infected sheep were exposed to the different concentrations of the extract (225, 450, 900 mg/mL) for 5-60 min in vitro and ex vivo. Then, by using the eosin exclusion assay, the viability of protoscoleces was studied.

Results: R. ribes extract had a potent protoscolicidal activity in vitro ; at 450 and 900 mg/ml, it killed 56.3 and 100% of protoscoleces, respectively, after 10 min of exposure. In ex vivo assay, the extract needed more time to kill the protoscoleces than the in vitro; so that at the concentration of 900 mg/mL, all protoscoleces were killed after 15 minutes.

Conclusion: The obtained results exhibited the potent protoscolicidal effects of R. ribes extract, particularly at the concentration of 900 mg/ml, which completely killed the parasite after <15 min of exposure. However, more supplementary studies are required to verify these findings by assessing animal models and clinical subjects.
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http://dx.doi.org/10.2174/1871526520666201116094851DOI Listing
October 2021

and scolicidal effects of L. to inactivate the protoscolecs during hydatid cyst surgery.

Ann Med Surg (Lond) 2019 Jun 24;42:7-10. Epub 2019 Apr 24.

Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Nowadays, surgery is considered as one of the most important treatments for hydatidosis. Due to laceration, the cyst and spread of the content within it (protoscoleces) during the surgery that can put the patient at the risk of re-infection, anaphylaxis shock and even death, surgeons use some chemical drugs as protoscolicidal agents. The study is aimed to evaluate the scolicidal effects of olive ( L.) leaf extract on hydatid cyst protoscoleces and .

Methods: After the collection of protoscoleces from sheep livers infected with fertile hydatid cysts, they were treated with various concentrations of olive leave extract (75-300 mg/mL) for 5-30 min and . Finally, the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining).

Results: The mean of the mortality of protoscoleces was 100% after 10 min of incubation with the concentration of 300 mg/ml of leaves extract. On the other hand, the mean of the mortality of protoscoleces after 20 min of incubation with the concentration of 150 mg/ml of leaves extract was 100%. After injection of leaves extract directly into the hydatid cyst (), the mean of the mortality of protoscoleces was 100% after 12 and 25 min of incubation with the concentration of 300 and 150 mg/ml of leaves extract, respectively; indicating that the extract requiring a further time to display a potent protoscolicidal effects.

Conclusion: Based on the findings of the study, it can be concluded that the extract of olive leaf had a significant scolicidal activity on hydatid cyst protoscoleces. However, further research, especially in human and animal subjects, are required to reach this conclusion.
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http://dx.doi.org/10.1016/j.amsu.2019.04.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527941PMC
June 2019
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