Publications by authors named "Mojgan Aryaeipour"

17 Publications

  • Page 1 of 1

Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Sensu Stricto Complex.

Iran J Public Health 2020 Sep;49(9):1758-1768

Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Background: Human a cyclo-zoonotic infection caused by tapeworms of the sensu stricto complex. The detection of mitochondrial genome data of genus can reflect the taxonomic status, genetic diversity, and population structure genetics.

Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 () gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity.

Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified.

Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran.
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http://dx.doi.org/10.18502/ijph.v49i9.4097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7898111PMC
September 2020

Identification and Phylogenetic Classification of species Isolated from Sheep and Cattle by PCR-RFLP in Zabol, in Sistan and Baluchistan Province, Southeast Iran.

Iran J Public Health 2019 May;48(5):934-942

Department of Medical Parasitology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.

Background: The detection of species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related genotypes by restriction fragment length polymorphism PCR.

Methods: Seventy adult flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, south-east Iran (Jan-Jul 2017). species were determined based on molecular features. For molecular detection, ITS1 region was amplified and sequenced. A 700 bp fragment was amplified. These were digested with RasΙ enzyme. specific fragments were 47, 59, 68, 104, and 370, while those related to had 45, 55, 170, 370.

Results: The two main species of and are responsible for fasciolosis in sheep and cattle in our region. From 35 isolated from cattle, 3 and 32 were and respectively. From 35 isolated from sheep, 4 were and 31 were

Conclusion: All Seventy samples from two different hosts (cattle and sheep) were identified as either or by PCR-RFLP. Genotypic variability of species was high in our region. It is recommended to assess molecular variation of isolates in other host livestock.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717417PMC
May 2019

Epidemiology of Fascioliasis in Kermanshah Province, Western Iran.

Iran J Public Health 2018 Jul;47(7):967-972

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: We aimed to determine the prevalence of fasciolosis in the definitive hosts (human and livestock) and intermediate ( snails) hosts in Kermanshah Province, western Iran from 2014-2016.

Methods: The study on animals was descriptive and retrospective one. All daily records of animals slaughtered in the abattoirs were analyzed. For the study of human fascioliasis, 975 serum samples were collected from different parts of Kermanshah Province and analyzed using ELISA based on excretory-secretory antigens. Moreover, 4400 snails were collected from 25 habitats. The snails were identified and examined for presence of cercariae by shedding method.

Results: Fasciolosis was diagnosed in 1.7% of slaughtered animals, which was significantly greater than the other species (<0.005). There was significant difference (<0.001) between the prevalence of fasciolosis and seasonal pattern. As for human cases, five cases (0.5%) were positive for fascioliasis. Regarding the seropositivity to fasciolosis, no significant differences were found for age groups, sex, level of education and occupation. No infection was seen in snails of the family Lymnaeidae.

Conclusion: The prevalence of parasite was lower compared to other provinces. This is probably due to sequential decline in rainfall and hot climate that makes conditions difficult for the snail intermediate host snails and the larval stages of fasciolid trematodes. The habitual food of people is another important point.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119572PMC
July 2018

Molecular Evidence of Human Fasciolosis Due to in Iran: A Case Report.

Iran J Public Health 2018 May;47(5):750-754

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Fascioliasis is a foodborne zoonotic disease caused by the two parasite species and . In spite of the presence of both species of in the livestock, to our knowledge, to date, no cases of human infection have been reported in Iran officially. Here, we report such a case in a 25 yr old woman referred to The Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran in 2016. CT imaging and MRCP revealed an ill-defined lesion of segments of liver. Specific ELISA produced a positive result besides detecting egg of the parasite via stool exam. The identification of parasite species was performed by the DNA extracted from the eggs and sequencing ITS-1, in addition to comparison to GenBank retrieved sequences, using the BLAST search tool. The sample showed 100% identity with . She was treated for fasciolosis with a single dose of Egaten® 10 mg/kg with positive response. This is the first case of human fasciolosis due to reported in Iran.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6005974PMC
May 2018

Seroprevalence of Human Cystic Echinococcosis in Alborz Province, Central Iran in 2015.

Iran J Public Health 2018 Apr;47(4):561-566

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: The aim of this study was to conduct a sero-epidemiological survey in Alborz Province, central Iran to detect the rate of hydatidosis in the city and nearby villages.

Methods: Overall, 680 serum samples were collected from 536 male and 127 female subjects referred to different health centers of Karaj, Alborz Province, central Iran and nearby villages in 2014-15. All patients filled out a questionnaire and an informed consent. Sera were analyzed using indirect-ELISA test with AgB. Ten μg/ml antigens (Proceeded hydatid fluid), serum dilutions of 1:500 and conjugate anti-human coombs with 1:10000 dilutions were utilized to perform the test. Data analysis was conducted using SPSS software ver. 11.5.

Results: Twenty-three cases (3.4%) were positive for hydatidosis by ELISA test. The prevalence of hydatidosis among females and males was 3.1% and 4.7%, respectively. The rate of the disease was significantly higher in areas where dogs were higher (<0.05). There was no significant difference as regards age groups, sex, job, residency, and literacy. Regarding occupation, housekeepers had the highest rate of infection as 5.9%. The seroprevalence of infection was 4.2% in bachelors and master people which showed the highest rate. As regards residency, urban life showed no significant difference with rural life (2.8% vs. 4.4%). Age group of 30-39 yr old, with 4.3% as prevalence had the highest rate of positivity (>0.05).

Conclusion: Because of the specific situation of Alborz Province, and availability of many stray dogs, obtained rate of hydatidosis shows that the authorities should be cautious to monitor the disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996319PMC
April 2018

Seroprevalence of Human Fasciolosis in Lorestan Province, Western Iran, in 2015-16.

Iran J Parasitol 2017 Jul-Sep;12(3):389-397

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: The aim of this study was the seroepidemiological survey for detecting the status of human fasciolosis in Lorestan Province, western Iran.

Methods: This cross-sectional study was conducted in 2015-16. Based on statistical estimations, 1256 serum samples were collected from different parts of Lorestan Province, western Iran, and stored at -20 °C until use. The collected serum samples were analyzed at Tehran University of Medical Sciences, Tehran, Iran using indirect ELISA method.

Results: Anti- antibodies were detected in 16 (1.3%) individuals. Regarding the seropositivity to fasciolosis, no significant differences were found between age groups, sex, level of education and occupation; however significant differences were observed regarding location, consuming local freshwater plants and water resources (<0.02.).

Conclusion: Local freshwater plants and unfiltered water resources were probably the main sources of the infection. Health education by local health centers to elevate awareness of people, and providing facilities for safer drinking water, especially in rural areas may help decrease the risk of fasciolosis infection in this region.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5623919PMC
October 2017

Molecular and Morphometrical Characterization of Species Isolated from Domestic Ruminants in Ardabil Province, Northwestern Iran.

Iran J Public Health 2017 Mar;46(3):318-325

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: We aimed to describe morphological and morphometrical characteristics of spp. in livestock from Ardabil Province, Northwest Iran.

Methods: Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as or based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified spp. based on morphological and metric assessment of external features of fresh adults, morphological and metric assessment of internal anatomy of stained mounted worms. Statistical analysis was conducted using the Student's t-test implemented in SPSS 15.0 (SPSS, Chicago, Illinois). Then the morphometric criteria of samples were compared with PCR-RFLP data. The results of PCR-RFLP were confirmed by COI gene sequence.

Results: The differences between the body length, area of the body, peripheral of the body, succer area, cone length, cone width, in two species were significant ( < 0.05). Based on Morphological characterizations, 6 specimens had the intermediate morphological features and 19 and 15 specimens had morphological features of and , respectively. In contrast, RFLP results showed, was present in 20 of the isolates, and in 20 isolates. No hybrid forms were detected.

Conclusion: PCR-RFLP method can be used for differentiation of species, which is more reliable method than morphology. Using morphology methods, merely, is not efficient for determination of genetic diversity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395527PMC
March 2017

Modulation of the Immune Response to DNA Vaccine Encoding Gene of 8-kDa Subunit of Antigen B Using Murine Interleukin-12 Plasmid in BALB/c Mice.

Iran J Parasitol 2016 Oct-Dec;11(4):480-489

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.

Background: The current study was designed to evaluate immune responses induced by DNA vaccines encoding 8-kDa subunit of antigen B (HydI) of and murine interleukin 12 (IL-12) as genetic adjuvants in BALB/c mice.

Methods: Expression plasmid pcDNA3.1 containing HydI (pcHyd1) as vaccine along with the murine interleukin 12 (pcMIL12) as adjuvant were used. Thirty-five mice in the five experimental groups received PBS, empty pcDNA3.1, pcHydІ, pcMIL-12, and pcHydІ+ pcMIL-12 in days zero, 14 and 28. Two weeks after the last immunization, evaluation of the immune response was performed by evaluating the proliferation of splenic lymphocytes, IFN-γ and IL-4, determination of IgG isotyping titer.

Results: Mice that received the pcHydI+pcMIL12 exhibited higher levels of lymphocyte proliferation compared to mice that received the pcHydI alone (<0.001), and produced significantly more IFN-γ in comparison to other groups (< 0.001). In addition, they produced significantly less IL-4 than mice receiving the PBS and the empty plasmid (<0.023). The IgG2a levels were clearly higher in pcHydI+pcMIL12 group in comparison with the groups of pcHydI alone, empty plasmid, and PBS. In contrast, IgG1 was elevated in the group of pcHydI.

Conclusion: Co-delivery of IL-12 with DNA encoding 8-kDa subunit of antigen B was effective significantly in inducing the immune response in mice.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5251176PMC
January 2017

Molecular Characterization of Animal spp. Isolates from Kermanshah, Western Iran.

Iran J Public Health 2016 Oct;45(10):1315-1321

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.

Background: We evaluated the genetic diversity of samples identified morphologically as spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.

Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.

Results: In Kermanshah, was present in 96% of the samples, was found only in two cattle sample. No hybrid forms were detected in the present study.

Conclusion: Our results contribute to clarify the dark spots of genotyping in different parts of Iran.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5149495PMC
October 2016

Serological study of Human Fasciolosis in Patients Referring to the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran during 2008-2014.

Iran J Parasitol 2015 Oct-Dec;10(4):517-22

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Infectious Diseases, AJA University of Medical Sciences, Tehran, Iran.

Background: Fascioliasis is a zoonotic disease of livestock and human caused by Fasciola species. Here in, the results of serological evaluation of fascioliasis in people referring to the School of Public Health, Tehran University of Medical Sciences during 2008-2014 are presented.

Methods: Demographic characterizations, symptoms and eosinophil rate were registered for every patient. Using somatic antigen of Fasciola, ELISA was performed and the results were analyzed. Data of questioners were analyzed as well.

Results: Among 206 applicants, 24.8% were seropositive for fascioliasis, included 21% female and 28.3% male. Mean range of age of patients was between 13 to 67 yr. The highest rate of seropositivity was found among 20-30 yr old patients. Most of the patients had hypereosinophilia. All patients had history of eating raw vegetables, or drinking unsafe water. Patients were referring from different provinces of Iran, including Gilan, Mazandaran, Tehran, Ardabil, Khuzestan, Lorestan, North Khorasan, Kermanshah, Azerbaijan, Fars, Kordestan, Hamedan and Markazi.

Conclusion: During recent years, variety of provinces in Iran, where patients with fascioliasis are referred, has been increased. Patients coming from Gilan and Mazandaran provinces were referred early after the onset of their symptoms. Most probably, physicians in Gilan and Mazandaran are more alert on fascioliasis than other provinces. Previous wrong diagnosis was more common among patients referring from other provinces than Gilan and Mazandaran provinces.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4724826PMC
January 2016

Seroepidemiology of Human Hydatidosis Using AgB-ELISA Test in Isfahan City and Suburb Areas, Isfahan Province, Central Iran.

Iran J Public Health 2015 Sep;44(9):1219-24

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran ; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.

Background: The aim of this study was to conduct a sero-epidemiological survey in Isfahan City and suburb areas, central Iran to detect the rate of human hydatidosis using ELISA test.

Methods: Overall, 635 serum samples were collected from subjects referred to different health centers in urban and rural regions of the city. Sera were analyzed using Ag-B ELISA test. Ten μg/ml antigens, serum dilutions of 1:500 and conjugate anti-human coombs with 1:10000 dilutions were utilized to perform the test. All subjects filled out a questionnaire and an informed consent. Data analysis was conducted using SPSS 18 software. Cut-off was calculated as X+3 SD.

Results: Cut-off value was calculated 0.19. Seven cases (1.1%) were seropositive for hydatidosis by ELISA test. The sero-prevalence of hydatidosis was 0.27% among females and 2.24% among males (P=0.019). Age group of 60-69 years old, with 2.59% as prevalence had the highest rate of positivity. There was no significant difference as regards age groups, job, residency, contact by dog and literacy. According to job, self-employed people had the highest rate of infection as 3.05%. The sero-prevalence of infection was 1.14% in diploma and 1.13% in illiterates. As regards residency, urban life (1.49%) showed no significant difference with rural life.

Conclusion: The rate of prevalence in this region showed that necessary cautions should be taken into account to monitor the spread of human hydatidosis in this region. In comparison with other studies, the rate of infection was roughly less than other regions.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645779PMC
September 2015

Molecular Cloning and Expression an 8-kDa Subunit of Antigen B from G1 strain of Echinococcus granulosus.

Iran J Public Health 2015 Jul;44(7):962-8

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran ; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.

Background: Echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection caused by the larval stage of the dog taeniid tapeworm Echinococcus granulosus. Vaccination has been considered as one of the ways to prevent of hydatidosis in recent decades. The aim of this study was to construct a pcDNA3.1 eukaryotic expression vector containing the subunit 8-kDa antigen B (Hyd1) of E. granulosus (G1 strain) and investigate its capability to induce protein expression in mammalian cell line, as a basis toward developing a DNA vaccine against hydatidosis.

Methods: The coding sequence of HydI was amplified by PCR with the specific PCR primers from pQE/HydI, and then was sub-cloned into pcDNA3.1 plasmid as expression vector. The pcHyd1 plasmid was digested by restriction enzymes and amplified with the specific PCR primers to confirm cloning of this gene in pcDNA3 plasmid. In last step, the sub-cloned gene was expressed in mammalian cell line (NIH 3T3 cells).

Result: The subunit 8-kDa antigen B (Hyd1) was successfully sub-cloned in pcDNA3.1 and Hyd1 protein was expressed in eukaryotic cell confirmed by SDS-PAGE and Western blot.

Conclusion: Recombinant plasmid of pcDNA3.1 was successfully constructed and express of recombinant Hyd1 protein was confirmed. That is promising step for forthcoming measures on providing vaccine against human and animal hydatidosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645767PMC
July 2015

Genotyping and Phylogenetic Analysis of Fasciola Spp. Isolated from Sheep and Cattle Using PCR-RFLP in Ardabil Province, Northwestern Iran.

Iran J Public Health 2014 Oct;43(10):1364-71

3. Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran ; 4. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences , Tehran, Iran.

Background: The aim of this study was to detect the genotype of Fasciola spp. in Meshkin-Shahr, Ardabil Province, northwestern Iran in different hosts using PCR-RFLP.

Methods: The parasite hosts included cattle, and sheep. Overall, 70 adult flukes from livers of slaughtered animals were collected from the abattoirs of aforementioned area. The included 35 samples from infected sheep and 35 samples from 35 infected cattle. PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS 1) region from Fasciola species were used to conduct the study.

Results: The fragment of approximately 700bp in all of the Fasciola samples was amplified. PCR products of ITS 1 were subjected for digestion by restriction enzyme. RsaI restriction enzyme was selected for RFLP method that caused the separation specifically of Fasciola species. Amplicons with the sequences of F. hepatica had a pattern of about 360, 100, and 60 bp band size, whereas F. gigantica worms had a profile of 360, 170, and 60 bp in size, respectively. Results based on PCR-RFLP analysis were confirmed by sequence analysis of representative ITS 1 amplicons. No hybrid forms were detected in the present study. All sheep were infected with F. hepatica but cattle were infected with both species.

Conclusion: Both species of Fasciola are present in Ardabil. The method described here can be valuable for identification of Fasciola species in endemic parts for fasciolosis, regions with intermediate species and in that overlapping distribution area.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4441889PMC
October 2014

Seroprevalence of human fascioliasis using indirect ELISA in isfahan district, central iran in 2013.

Iran J Parasitol 2014 Oct-Dec;9(4):461-5

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran ; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.

Background: The aim of this study was to detect the seroprevalence of human fascioliasis in Isfahan County, central Iran in 2013.

Methods: Overall, 471 sera samples were collected from people recalled randomly to 20 health centers in the city of Isfahan and 10 related villages in 2014. Sera were examined using ELISA test. A questionnaire was filled out for each participant.

Results: Altogether eight cases (1.7%) were seropositive which had the OD absorbance in ELISA test more than the calculated cutoff of 0.36. All of them were female. One positive subject had a history of consuming Delar (Local dish) and three seropositive cases with history of eating Zeitoon-Parvadeh (Proceeded olive).

Conclusion: Isfahan County might be considered as one area apt for fascioliasis. More studies in terms of veterinary investigation and verifying the risk factors are necessary.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345084PMC
March 2015

Seroprevalence of human fascioliasis in meshkin-shahr district, ardabil province, northwestern iran in 2012.

Iran J Parasitol 2013 Oct;8(4):516-21

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.

Background: The aim of this study was to conduct a seroprevalence survey in Meshkin-Shahr, Ardabil Province, north western Iran to detect the rate of human fascioliasis in the city and nearby villages. Literature shows that no such study has been conducted so far.

Methods: Overall, 458 serum samples were collected by randomized cluster sampling method from 153 males and 305 females referred to different health centers of the region after recalling by staff in those centers in 2012. All cases filled out a questionnaire and an informed consent. Sera were analyzed using indirect-ELISA test. Ten μg /ml antigens (Liver Fluke Homogenate), serum dilutions of 1:500 and conjugate anti-human coombs with 1:10000 dilutions were utilized to perform the test. Data analysis was conducted using SPSS software ver. 18.

Results: Nine cases (1.96%) were positive for fascioliasis by ELISA test. The seroprevalence of fascioliasis among females was 1.63% and 2.6% in males. There was no significant difference as regards age groups, sex, job, residency, literacy and consuming row vegetable. According to job, unemployment subjects had the highest rate of infection as 5.9%. The seroprevalence of infection was 1.52% in illiterate people. As for residency, urban life showed no significant difference with rural life (2.4% vs 1.42). Age group of 40-49 yr old, with 3.3% seropositivity had the highest rate.

Conclusion: Obtained seroprevalence of fascioliasis shows immediate attention of health authorities to the diseases in the area. The adjacent of Ardabil Province to endemic areas of fasciolosis accentuates this attention.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4266114PMC
October 2013

Seroepidemiology of Human Hydatidosis Using AgB-ELISA Test in Arak, Central Iran.

Iran J Public Health 2013 1;42(4):391-6. Epub 2013 Apr 1.

Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.

Background: On the continuation of sorting the puzzle of the situation of hydatid disease in Iran and considering that so far no survey was conducted in this context in Arak City, Markazi Province central Iran, the present study was conducted to determine the seroprevalence of human hydatidosis using AgB-ELISA test.

Methods: Totally 578 serum samples randomly were collected from patients referred to hospitals and different health centers in the city and 3 nearby villages of Arak. All sera were examined by ELISA tests using AgB. Before sampling, a questionnaire was filled out for each case. Data were analyzed using Chi-square test and multivariate logistic regression for risk factors analysis. P<0.05 was considered significant.

Results: Cut-off value was calculated 0.32. Twenty cases (3.46%) were seropositive for hydatidosis in the region. This rate for females was 3.99% and for males 2.26%, respectively. There was no significant difference as regards all factors studied except location (P<0.001). As for job, farmers and ranchmen had the highest rate of infection as 6.67%. The seropositivity rate infection was 4.8% in illiterate people, which showed the highest rate. As regards residency, rural life showed significant difference with urban life (1.5% vs. 7 %). Age group of 40-49 yr old (6.25%) had the highest rate of positivity.

Conclusion: The rate of prevalence in this region shows more or less the same range with other cities of Iran. Obtained result might assist the policy makers to take sanitary measures to control the disease.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684725PMC
June 2013