Dr Mojdeh Hakemi Vala, PhD - Shahid Beheshti  University of Medical Sciences - Associate profesor

Dr Mojdeh Hakemi Vala

PhD

Shahid Beheshti University of Medical Sciences

Associate profesor

Tehran | Iran (Islamic Republic of)

Main Specialties: Medical Microbiology, Molecular Genetic Pathology

Additional Specialties: microbiologist

ORCID logohttps://orcid.org/0000-0002-8355-6885

Dr Mojdeh Hakemi Vala, PhD - Shahid Beheshti  University of Medical Sciences - Associate profesor

Dr Mojdeh Hakemi Vala

PhD

Introduction

Primary Affiliation: Shahid Beheshti University of Medical Sciences - Tehran , Iran (Islamic Republic of)

Specialties:

Additional Specialties:


View Dr Mojdeh Hakemi Vala’s Resume / CV

Education

Oct 2019
Melbourn University of Medical Sciences
Traininig(6months)
Microbiology
Jan 2001 - Jan 2006
Iran university of medical sciences
PhD
Microbiology

Experience

Jan 2009
Shahid Beheshti University of Medical Sciences School of Medicine

Microbiology

Publications

29Publications

66Reads

22Profile Views

3PubMed Central Citations

Evaluation of Clarithromycin Resistance Among Iranian Helicobacter pylori Isolates by E-Test and Real-Time Polymerase Chain Reaction Methods

2016 Mar 19;9(5):e29839

Jundishapur J Microbiol.

BACKGROUND: Helicobacter pylori is an important pathogen of human gastric mucosa. Antibiotic resistance, especially resistance to clarithromycin is a major factor for treatment failure of H. pylori infections. The main mechanism of clarithromycin resistance in these bacteria is related to point mutations in three different locations of 23S rRNA gene. OBJECTIVES: The aims of this study were to evaluate the resistance rate to clarithromycin among local H. pylori isolates by the E-test method and to determine the profile of point mutation in 23S rRNA by real-time polymerase chain reaction (PCR) method. PATIENTS AND METHODS: Eighty biopsy samples were collected from dyspeptic patients by endoscopy during 2011 - 2012. All samples were homogenized immediately and cultured on supplemented brucella blood agar and incubated under microaerophilic conditions. Further biochemical tests and ureC gene PCR was done for H. pylori confirmation. The H. pylori OC1096 strain was used as the control strain, simultaneously. Frequency of clarithromycin resistance was determined by the E-test method based on the clinical and laboratory standard institute (CLSI) standards. Point mutation profile was determined by real-time PCR and further analysis of melting curve, amplicon sequencing was done continuously. RESULTS: From 80 biopsy samples, 20 positive H. pylori isolates were detected and confirmed by biochemical tests and PCR method. Overall, 21.7% of the H. pylori isolates, showed clarithromycin resistance phenotype by use of the E-test. Also, the minimal inhibitory concentration of clarithromycin was determined as ≥ 0.5 mg/L by the E-test method. Only point mutation in the location of A2143G with melting temperature of 54.7°C was observed in all resistant isolates. CONCLUSIONS: This study showed that the frequency of H. pylori clarithromycin resistance in Iran is relatively high. Since clarithromycin is not commonly used in Iran for H. pylori eradication, the high rate of resistance could be related to cross-reactivity between other macrolides. Therefore, macrolide antibiotics must be prescribed with precaution in any case of treatment other than H. pylori infections. All resistant isolates showed A2143G mutation in 23S rRNA as the dominant pattern of point mutation at least in Tehran H. pylori isolates.

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March 2016
7 Reads

Efflux Pump Inhibitor Phenylalanine-Arginine Β-Naphthylamide Effect on the Minimum Inhibitory Concentration of Imipenem in Acinetobacter baumannii Strains Isolated From Hospitalized Patients in Shahid Motahari Burn Hospital, Tehran, Iran.

Jundishapur J Microbiol 2015 Oct 12;8(10):e19048. Epub 2015 Oct 12.

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran.

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http://dx.doi.org/10.5812/jjm.19048DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4639875PMC
October 2015
30 Reads
2 Citations
0.780 Impact Factor

Occurrence of Potentially Pathogenic Bacterial-Endosymbionts in Acanthamoeba Spp.

Iran J Parasitol 2015 Apr-Jun;10(2):181-8

Dept. of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522293PMC
August 2015
8 Reads
1 Citation
0.872 Impact Factor

Antibacterial and antifungal activities of the endemic species Glaucium vitellinum Boiss. and Buhse.

Avicenna J Phytomed 2015 Jan-Feb;5(1):56-61

Department of Pharmacognosy, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran - Iran (IAUPS).

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352533PMC
March 2015
9 Reads

Search for Proteins in the Liquid Extract of Edible Mushroom, Agaricusbisporus, and Studying their Antibacterial Effects.

Iran J Pharm Res 2012 ;11(1):145-50

Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medeical Sciences, Tehran, Iran. ; Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813085PMC
November 2013
12 Reads

Comparing invasive and non-invasive of isolated Shigella flexneri by electron microscopy of cell culture, SDS-PAGE and Congo red method.

Iran Biomed J 2007 Jan;11(1):47-52

Dept. of Microbiology, Pasture Institute of Iran, Tehran, Iran.

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January 2007
7 Reads

Professor

1969
5 Reads

Top co-authors

Mojdeh Hakemi Vala
Mojdeh Hakemi Vala

Shahid Beheshti University of Medical Sciences

2
Hadi Mehregan
Hadi Mehregan

Shahid Beheshti University of Medical Sciences

1
Jamileh Nowroozi
Jamileh Nowroozi

Islamic Azad University

1
Saeed Haghighi
Saeed Haghighi

Quality Control laboratory

1
Farideh Ghazi
Farideh Ghazi

National Research Institute of Tuberculosis and Lung Disease

1