Publications by authors named "Mohd Khalizan Sabullah"

6 Publications

  • Page 1 of 1

A Review: Underutilized Plant of Sabah and its Potential Value.

Curr Pharm Biotechnol 2022 ;23(1):47-59

Faculty of Science and Natural Resources, University Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah,Malaysia.

Underutilized plants are referred to a plant species whose potential is not fully utilized yet and they are usually found abundantly in certain local areas but are globally rare. Sabah is known for high biodiversity and contains many underutilized plants. To our knowledge, this is the first review to provide overview information of the medicinal value and pharmacological properties of underutilized plants in Sabah. Extract and metabolites in different parts of several underutilized plants contain multiple beneficial bioactive compounds and the exploitation of these compounds was supported by additional data that plays various biological activities, including anti-atherosclerotic, anti-cancer antihypercholesterolemic and anti-ulcerogenic. A handful of pharmacological studies on these underutilized plants have conclusively outlined the mode of action in treatment of several diseases and in other health aspects. This paper limits its scope to review and highlight the potential of using underutilized plants in Sabah only which could serve as reliable resource for health product development in pharmaceutical and nutraceutical through continuous discovering of more active and sustainable resources as well as ingredients for food and medicine.
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http://dx.doi.org/10.2174/1389201022666210208201212DOI Listing
January 2022

In vivo and in vitro effects on cholinesterase of blood of by copper.

3 Biotech 2019 Feb 2;9(2):64. Epub 2019 Feb 2.

1Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor Malaysia.

The present study is aimed to evaluate the effects of sub-acute toxicity testing of copper sulphate (CuSO), on behavioural, histological and biochemical changes of the (black tilapia) blood tissues. The effects were assessed according to the previous results on sub-acute toxicity test after exposing fish to several concentrations (0.0, 2.5, 5.0, and 10.0 mg/L). The observations of scanning electron microscope, and transmission electron microscope studies revealed severe histopathological changes on the surface and the cellular changes in blood tissues, respectively. The morphological alterations in blood involved irregular structure of red blood cell and blood clot formation. CuSO affected the biochemical alteration of the blood cholinesterase also known as serum cholinesterase (ChE). Blood ChE inhibited up to 80% of activity when exposed to 10.0 mg/L CuSO. The findings from this study can further improve the quality standards of aquaculture industry and the fundamental basis in selecting suitable strains among freshwater fish species to be used as bioindicator.
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http://dx.doi.org/10.1007/s13205-019-1592-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361190PMC
February 2019

Toxic effects of copper on liver and cholinesterase of Clarias gariepinus.

Environ Sci Pollut Res Int 2017 Oct 13;24(28):22510-22523. Epub 2017 Aug 13.

Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor, Malaysia.

The release of pollutants, especially heavy metals, into the aquatic environment is known to have detrimental effects on such an environment and on living organisms including humans when those pollutants are allowed to enter the food chain. The aim of this study is to analyse the damage to Clarias gariepinus' liver caused by exposure to different concentrations of copper. In the present study, samples of C. gariepinus were exposed to sub-lethal copper sulphate (CuSO) concentrations (from 0.2 to 20.0 mg/L) for 96 h. Physiological and behavioural alterations were observed with respect to their swimming pattern, mucus secretion and skin colour. Mortality was also observed at high concentrations of copper. Histopathological alterations of the liver were analysed under light, transmission and scanning electron microscopies. The liver of the untreated group showed normal tissue structures, while histopathological abnormalities were observed in the treated fish under light and electron microscopes with increased copper concentrations. Histopathological abnormalities include necrosis, melanomacrophage, hepatic fibrosis and congested blood vessels. In addition, the enzyme activity of liver cholinesterase (ChE) was also found to be affected by copper sulphate, as 100% of cholinesterase activity was inhibited at 20.0 mg/L. Thus, liver enzyme activity and histopathological changes are proven to be alternative sources for biomarkers of metal toxicity.
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http://dx.doi.org/10.1007/s11356-017-9923-3DOI Listing
October 2017

Evaluation of acetylcholinesterase source from fish, Tor tambroides for detection of carbamate.

J Environ Biol 2016 Jul;37(4):479-84

Acetylcholinesterase (AChE) from the brain tissue of local freshwater fish, Tor tambroides was isolated through affinity purification. Acetylthiocholine iodide (ATCi) was preferable synthetic substrate to purified AChE with highest maximal velocity (V(max)) and lowest biomolecular constant (K(m)) at 113.60 Umg(-1) and 0.0689 mM, respectively, with highest catalytic efficiency ratio (V(max)/K(m)) of 1648.77. The optimum pH was 7.5 with sodium phosphate buffer as medium, while optimal temperature was in the range of 25 to 35 degrees C. Bendiocarp, carbofuran, carbaryl, methomyl and propoxur significantly lowered the AChE activity greater than 50%, and the IC50 value was estimated at inhibitor concentration of 0.0758, 0.0643, 0.0555, 0.0817 and 0.0538 ppm, respectively.
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July 2016

Anti-HMG-CoA Reductase, Antioxidant, and Anti-Inflammatory Activities of Amaranthus viridis Leaf Extract as a Potential Treatment for Hypercholesterolemia.

Evid Based Complement Alternat Med 2016 9;2016:8090841. Epub 2016 Mar 9.

Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

Inflammation and oxidative stress are believed to contribute to the pathology of several chronic diseases including hypercholesterolemia (elevated levels of cholesterol in blood) and atherosclerosis. HMG-CoA reductase inhibitors of plant origin are needed as synthetic drugs, such as statins, which are known to cause adverse effects on the liver and muscles. Amaranthus viridis (A. viridis) has been used from ancient times for its supposedly medically beneficial properties. In the current study, different parts of A. viridis (leaf, stem, and seed) were evaluated for potential anti-HMG-CoA reductase, antioxidant, and anti-inflammatory activities. The putative HMG-CoA reductase inhibitory activity of A. viridis extracts at different concentrations was determined spectrophotometrically by NADPH oxidation, using HMG-CoA as substrate. A. viridis leaf extract revealed the highest HMG-CoA reductase inhibitory effect at about 71%, with noncompetitive inhibition in Lineweaver-Burk plot analysis. The leaf extract showed good inhibition of hydroperoxides, 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and ferric ion radicals in various concentrations. A. viridis leaf extract was proven to be an effective inhibitor of hyaluronidase, lipoxygenase, and xanthine oxidase enzymes. The experimental data suggest that A. viridis leaf extract is a source of potent antioxidant and anti-inflammatory agent and may modulate cholesterol metabolism by inhibition of HMG-CoA reductase.
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http://dx.doi.org/10.1155/2016/8090841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804040PMC
April 2016

The assessment of cholinesterase from the liver of Puntius javanicus as detection of metal ions.

ScientificWorldJournal 2014 27;2014:571094. Epub 2014 Oct 27.

Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and -25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions.
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http://dx.doi.org/10.1155/2014/571094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225846PMC
November 2015
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