Publications by authors named "Mohammed Ishaq"

21 Publications

  • Page 1 of 1

Granular cell tumor of the bronchus: A case report.

Int J Surg Case Rep 2022 Jan 20;90:106704. Epub 2021 Dec 20.

King Saud Medical City, Riyadh, Saudi Arabia.

Introduction And Importance: Pulmonary granular cell tumor (GCT) is a very rare neoplasm that originates from Schwann cells.

Case Presentation: Our case report describes a symptomatic benign pulmonary GCT found during follow-up CT imaging and was eventually excised with a good outcome.

Clinical Discussion: GCT are mostly benign tumors. Pulmonary GCTs mostly occur in the endobronchial region and can be symptomatic or discovered incidentally.

Conclusion: Treatment options for pulmonary GCT are either conservative treatment or surgical resection depending on the size of the tumor and the presence of tracheal wall invasion.
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http://dx.doi.org/10.1016/j.ijscr.2021.106704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715071PMC
January 2022

Long-term Outcome following Percutaneous Intervention of Intra-stent Coronary Occlusion and Evaluating the Different Treatment Modalities.

Int J Cardiol Heart Vasc 2021 Jun 31;34:100803. Epub 2021 May 31.

Heartlands Hospital, University Hospitals Birmingham, United Kingdom.

Background: Angioplasty for ISR remains a challenge with relatively high rates of recurrence. Although there is a plethora of data on ISR, there is relatively less data on intra-stent-CTO. In this study, we explore the long-term clinical outcomes following angioplasty to intra-stent CTO and study the differences in clinical outcomes between three treatment-arms: POBA vs. DES vs. DCB.

Methods And Results: We evaluated all patients who underwent PCI to intra-stent CTO between 2011 and 2017. The endpoints used were: cardiac-death, TVMI, TLR, TVR, and MACE.During the study period, 403-patients with a mean age of 69.2 years had successful PCI to intra-stent CTO. 50% were diabetic, 38% had CKD and 32% had left ventricular dysfunction. 93% of cases were stable angina. 22% (n = 88) received only POBA, 28% (n = 113) received DCB and 50% (n = 202) received DES. During the median follow-up of 48-months, cardiac-death occurred in 5.8% (n = 23), TVMI in 4% (n = 16), TLR in 45.6% (n = 182), TVR in 48.7% (n = 194) and MACE of 46%. There were no differences in the hard endpoints between the 3treatment arms. However, the TLR and overall MACE were better in DCB and DES-groups as compared to POBA (TLR: 33%vs.42%vs.49%; p = 0.06); MACE (34% vs. 45% vs. 52%; p = 0.05).

Conclusion: This is the first study that has focussed on the outcomes following angioplasty to intra-stent CTOs with a very long-term follow-up. The hard endpoints were low, although the TLR rates were high. In regards to treatment strategy, the DCB and DES provide relatively better outcomes than POBA.
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http://dx.doi.org/10.1016/j.ijcha.2021.100803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245734PMC
June 2021

Indoor transmission dynamics of expired SARS-CoV-2 virus in a model African hospital ward.

J Environ Health Sci Eng 2021 Jun 22;19(1):331-341. Epub 2021 Jan 22.

Environmental Engineering Research Laboratory, Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria.

Cough and sneeze droplets' interactions with indoor air of a typical hospital clinic that could be majorly found in developing African countries were studied to investigate the effectiveness of existing guidelines/protocols being adopted in the control of the widespread coronavirus disease (COVID-19) transmission. The influences of indoor air velocity, the type, size distribution, residence time in air, and trajectory of the droplets, were all considered while interrogating the effectiveness of physical distancing measures, the use of face covers, cautionary activities of the general public, and the plausibility of community spread of the SARS-CoV-2 virus through airborne transmission. Series of 3-D, coupled, discrete phase models (DPM) were implemented in the numerical studies. Based on DPM concentration maps as function of particle positions and particle residence times that were observed under different droplets release conditions, the virus-laden droplets could travel several meters away from the source of release (index patient), with smaller-sized particles staying longer in the air. The behavior of indoor air was also found to indicate complex dynamics as particle transports showed no linear dependence on air velocity.

Supplementary Information: The online version contains supplementary material available at 10.1007/s40201-020-00606-5.
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http://dx.doi.org/10.1007/s40201-020-00606-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821173PMC
June 2021

Selected Heavy Metals Removal From Electroplating Wastewater by Purified and Polyhydroxylbutyrate Functionalized Carbon Nanotubes Adsorbents.

Sci Rep 2019 03 14;9(1):4475. Epub 2019 Mar 14.

Department of Physics, University of the Free State, P.O. Box 339, ZA-9300, Bloemfontein, Republic of South Africa.

This research investigated the removal of heavy metals (As, Pb, Cr, Cd, Ni, Cu, Fe, and Zn) via batch adsorption process from industrial electroplating wastewater using two different nano-adsorbents; purified carbon nanotubes (P-CNTs) and polyhydroxylbutyrate functionalized carbon nanotubes (PHB-CNTs), both produced through catalytic chemical vapour deposition (CCVD) method. HRSEM, HRTEM, XRD, DLS, BET, FTIR, XPS, TGA, pH drift and Raman spectroscopy were used to characterize the developed nano-adsorbents. In the batch adsorption process, the effects of contact time, dosage, temperature and pH were studied. Both nano-adsorbents gave optimum contact time, equilibrium time, optimum dosage, and pH of 10 minutes, 70 minutes, 20 mg, and 5.63-5.65 respectively. The heavy metals removal efficiencies by the nano-adsorbents followed the order of PHB-CNTs > P-CNTs based on ion exchange and electrostatic forces mechanism. For P-CNTs and PHB-CNTs, the equilibrium sorption isotherm suits temkin model, kinetic data fitted to pseudo-second order based on the linear regression correlation coefficient, and the thermodynamic study established spontaneity and endothermic nature of the adsorption process. The findings in this research conclude that both nano-adsorbents have exceptional capacity to remove heavy metals from the adsorbate, with PHB-CNTs possessing better quality. The treated adsorbate meets the standard for industrial or irrigation re-use.
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http://dx.doi.org/10.1038/s41598-018-37899-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418241PMC
March 2019

Association and gene-gene interaction analyses for polymorphic variants in CTLA-4 and FOXP3 genes: role in susceptibility to autoimmune thyroid disease.

Endocrine 2019 06 15;64(3):591-604. Epub 2019 Feb 15.

Department of Genetics, Osmania University, Hyderabad, Telangana, India.

Purpose: Polymorphic variants of cytotoxic T-lymphocyte antigen-4 (CTLA-4) and forkhead box protein P3 (FOXP3) genes are implicated in dysregulated immune homeostasis and autoimmune disorders. We analyzed the association between CTLA-4 rs231775 and FOXP3 rs3761548, rs3761549 polymorphisms and predisposition to autoimmune thyroid disease (AITD), inclusive of Hashimoto's thyroiditis (HT) and Graves' disease (GD) in South-Indian population.

Methods: A total of 355 AITD subjects (comprising 275 HT and 80 GD) and 285 randomly selected age- and sex-matched control subjects were genotyped for the aforementioned polymorphisms by PCR-RFLP method.

Results: The rs231775 "G" allele was preponderant in HT and GD subjects when compared with controls and exerted a dominant influence on the susceptibility to HT (p = 0.009) and GD (p = 0.02), respectively. There was no allelic association of rs3761548 and rs3761549 polymorphisms with AITD susceptibility, albeit a significant difference in genotype distribution with respect to rs3761549. Haplotype analysis revealed an increased frequency of rs3761548 "C"-rs3761549 "T" in HT and GD subjects, thereby associating it with disease predisposition (p = 0.03). Epistatic interaction analysis by multifactor dimensionality reduction approach revealed redundancy between CTLA-4 and FOXP3 genes in influencing the susceptibility to AITD.

Conclusions: The genetic variation in CTLA-4 gene with reference to rs231775 polymorphism contributes to an increased predisposition to HT and GD. Also, in conjunction with FOXP3 gene variants it seems to influence the susceptibility to HT and GD respectively. The significance of these findings in combination with antithyroid antibody screening could plausibly contribute towards meticulous case-finding for effective treatment of HT and GD.
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http://dx.doi.org/10.1007/s12020-019-01859-3DOI Listing
June 2019

Unusual Malposition of a Chest Tube, Intrathoracic but Extrapleural.

Case Rep Radiol 2018 6;2018:8129341. Epub 2018 Aug 6.

Organ Transplant Centre, King Faisal Specialist Hospital & Research Centre (KFSH&RC), Riyadh, Saudi Arabia.

Chest tube malpositioning is reported to be the most common complication associated with tube thoracostomy. Intraparenchymal and intrafissural malpositions are the most commonly reported tube sites. We present a case about a 21-year-old patient with cystic fibrosis who was admitted due to bronchiectasis exacerbation and developed a right-sided pneumothorax for which a chest tube was inserted. Partial initial improvement in the pneumothorax was noted on the chest radiograph, after which the chest tube stopped functioning and the pneumothorax remained for 19 days. Chest computed tomography was done and revealed a malpositioned chest tube in the right side located inside the thoracic cavity but outside the pleural cavity (intrathoracic, extrapleural). The removed chest tube was patent with no obstructing materials in its lumen. A new thoracostomy tube was inserted and complete resolution of the pneumothorax followed.
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http://dx.doi.org/10.1155/2018/8129341DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6106908PMC
August 2018

Biomarkers of Prostatic Cancer: An Attempt to Categorize Patients into Prostatic Carcinoma, Benign Prostatic Hyperplasia, or Prostatitis Based on Serum Prostate Specific Antigen, Prostatic Acid Phosphatase, Calcium, and Phosphorus.

Prostate Cancer 2017 12;2017:5687212. Epub 2017 Jan 12.

Salar-e-Millat Research Centre, PEH, DCMS, Hyderabad, Telangana, India.

Prostatitis, BPH, and P.Ca are the most frequent pathologies of the prostate gland that are responsible for morbidity in men. Raised levels of PSA are seen in different pathological conditions involving the prostate. PAP levels are altered in inflammatory or infectious or abnormal growth of the prostate tissue. Serum calcium and phosphorus levels were also found to be altered in prostate cancer and BPH. The present study was carried out to study the levels of PSA, PAP, calcium, and phosphorus in serum of patients with Prostatitis, BPH, or P.Ca and also to evaluate the relationship between them. Males in the age group of 50-85 years with LUTS disease symptoms and with PSA levels more than 4 ng/mL were included. A total of 114 patients were analyzed including 30 controls. Prostatitis in 35.7% of cases, BPH in 35.7% of the cases, and P.Ca in 28.57% of the cases were observed. Thus, the nonmalignant cases constitute a majority. PSA, a marker specific for prostatic conditions, was significantly high in all the diseases compared to controls. A rise in serum PSA and PAP indicates prostatitis or, in combination with these two tests, decreased serum calcium shows advanced disease.
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http://dx.doi.org/10.1155/2017/5687212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5266858PMC
January 2017

Asymptomatic coronary artery spasm with acute pathological ST elevation on routine ECG: is it common?

BMJ Case Rep 2014 Aug 12;2014. Epub 2014 Aug 12.

Fairfield Hospital, Bury, UK.

Asymptomatic spontaneous coronary artery spasm is rare and there are no case reports in literature presenting with acute ST elevation on routine ECG. We present the case of a 68-year-old Caucasian man who presented to a primary care physician for a routine ECG as part of hypertension follow-up. ECG revealed ST elevation in inferior leads II, III and aVF with reciprocal ST depression in leads I, aVL and also ST depression in anterior leads V1, V2 and V3 suggesting ongoing inferoposterior ST elevation myocardial infarction. The patient was completely well, stable and asymptomatic and he was rushed immediately to the coronary care unit via emergency ambulance. The patient was subjected to a battery of urgent investigations which were all normal. Also an urgent coronary angiogram was undertaken which showed completely normal coronary anatomy.
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http://dx.doi.org/10.1136/bcr-2013-202586DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139574PMC
August 2014

Polymorphisms in oxidative stress pathway genes and risk of diabetic nephropathy in South Indian type 2 diabetic patients.

Nephrology (Carlton) 2014 Oct;19(10):623-9

Department of Genetics, Osmania University, Hyderabad, India.

Aim: Diabetic nephropathy (DN), a common microvascular complication of type 2 diabetes mellitus (T2DM) is polygenic, with a vast array of genes contributing to disease susceptibility. Accordingly, we explored the association between DN and six polymorphisms in oxidative stress related genes, namely eNOS, p22phox subunit of NAD(P)H oxidase, PARP-1 and XRCC1 in South Indian T2DM subjects.

Methods: The study included 155 T2DM subjects with DN and 162 T2DM patients with no evidence of DN. The selected polymorphisms were genotyped by polymerase chain reaction and Taqman allele discrimination assay.

Results: No significant difference was observed in the genotype and allele distribution of eNOS -786T > C, intron 4a4b, p22phox 242C > T and XRCC1 Arg399Gln polymorphisms between T2DM groups with and without DN. Contrastingly, there appeared to be a significant association of eNOS 894G > T and PARP-1 Val762Ala polymorphisms with DN wherein, the presence of 894T allele was associated with an enhanced risk for DN [P = 0.005; OR = 1.78 (1.17-2.7)], while the 762Ala allele seemed to confer significant protection against DN [P = 0.02; OR = 0.59 (0.37-0.92)]. Multiple logistic regression analysis revealed a significant and independent association of eNOS 894G > T, PARP-1 Val762Ala polymorphisms and hypertension with DN in T2DM individuals.

Conclusions: eNOS 894G > T and PARP-1 Val762Ala polymorphisms appeared to associate significantly with DN, with the former contributing to an enhanced risk and the latter to a reduced susceptibility to DN in South Indian T2DM individuals.
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http://dx.doi.org/10.1111/nep.12293DOI Listing
October 2014

A young pregnant woman with spontaneous carotid artery dissection--unknown mechanisms.

BMJ Case Rep 2014 May 30;2014. Epub 2014 May 30.

Stafford Hospital, Stafford, UK.

Spontaneous carotid artery dissection in pregnancy has not been reported before. We present a case of a 31-year-old Caucasian woman who was 11 weeks pregnant and presented with neck pain, headache, vomiting and left side Horner's syndrome. Subsequent investigations with MR angiography confirmed spontaneous left internal carotid artery dissection.
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http://dx.doi.org/10.1136/bcr-2013-202541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039886PMC
May 2014

Association Analysis of Polymorphisms in Genes Related to Oxidative Stress in South Indian Type 2 Diabetic Patients with Retinopathy.

Ophthalmic Genet 2016 12;37(1):1-8. Epub 2014 Mar 12.

a Department of Genetics , Osmania University , Hyderabad , India and.

Background: Diabetic Retinopathy (DR) is one of the most common microvascular complications of type 2 diabetes mellitus (T2DM) and is polygenic with a multitude of genes contributing to disease susceptibility. The present study aimed at exploring the association between DR and seven polymorphisms in oxidative stress-related genes, i.e. ACE, eNOS, p22phox subunit of NAD(P)H oxidase, PARP-1 and XRCC1 in South Indian T2DM subjects.

Materials And Methods: The study included 149 T2DM subjects with DR (diagnosed through funduscopic examination) and 162 T2DM patients with no evidence of DR. The selected polymorphisms were genotyped by polymerase chain reaction (PCR) and Taqman allele discrimination assay.

Results: There was no significant difference in the genotype and allele distribution of ACE ins/del, eNOS-786T>C, 894G>T, 4a4b and p22phox 242C>T polymorphisms between T2DM groups with and without DR. Contrastingly, there appeared to be a significant association of PARP-1 Val762Ala and XRCC1 Arg399Gln polymorphisms with DR, wherein 762Ala allele seemed to confer significant protection against DR (p = 0.01; OR = 0.51 [0.3-0.86]), while the presence of 399Gln allele was associated with an enhanced risk for DR (p = 0.02; OR = 1.52 [1.07-2.15]). Multiple logistic regression analysis revealed a significant and independent association of Val762Ala and Arg399Gln polymorphisms and other putative risk factors with DR in T2DM individuals.

Conclusions: The polymorphisms in the DNA repair genes PARP-1 and XRCC1 tended to associate significantly with DR. While Val762Ala polymorphism was associated with reduced susceptibility to DR, the Arg399Gln polymorphism contributed an elevated to risk for DR in South-Indian T2DM individuals.
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http://dx.doi.org/10.3109/13816810.2014.895015DOI Listing
September 2016

An unusual intracardiac electrogram showing cause for false electrical discharge from an ICD.

BMJ Case Rep 2013 Aug 2;2013. Epub 2013 Aug 2.

Department of Cardiology/Medicine, West Midlands Deanery, Birmingham, UK.

We describe a rare case of inappropriate implantable cardioverter defibrillator (ICD) therapy due to false sensing of electromechanical interference from diathermy as 'ventricular fibrillation (VF)'. This occurred during surgical removal of sternal wires under general anaesthesia. Postsurgical interrogation of ICD revealed the intracardiac electrogram showing the mechanical interference sensed as 'VF' by ICD and subsequent shock delivery.
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http://dx.doi.org/10.1136/bcr-2013-010202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3761679PMC
August 2013

Association of FOXP3 (rs3761548) promoter polymorphism with nondermatomal vitiligo: A study from India.

J Am Acad Dermatol 2013 Aug 14;69(2):262-6. Epub 2013 Mar 14.

Department of Genetics, Osmania University, Hyderabad, India.

Background: The rs3761548 polymorphism (-3279 C>A) of FOXP3 gene is associated with several autoimmune disorders.

Objective: We sought to determine whether rs3761548 polymorphism is associated with nondermatomal vitiligo in Indian subjects.

Methods: Genomic DNA was isolated from blood samples of 303 patients and 305 control subjects and genotyping was done by allele-specific primers. Data analysis was carried out for the entire cohort and separately for male and female participants as FOXP3 is an X-linked marker. Statistics were performed using software.

Results: The genotype frequencies differed significantly from patients to control subjects (P = .002). Further analysis demonstrated female participants with CC genotype were protected (CC vs CA+AA; odds ratio 0.38, 95% confidence interval 0.238-0.615) and those with CA genotype were at higher risk to develop vitiligo (CA vs CC+AA; odds ratio 2.634, 95% confidence interval 1.604-4.325). However, no such statistical difference was observed in male participants.

Limitations: Our study is, to our knowledge, the first report from India with respect to vitiligo and rs3761548; however, we lack adequate literature assistance.

Conclusions: The rs3761548 of FOXP3 gene in our population may be associated with susceptibility to vitiligo because of altered expression. CC genotype appears to be protective and CA genotype seems to impart nearly 3-fold risk to develop vitiligo in women and girls.
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http://dx.doi.org/10.1016/j.jaad.2013.01.035DOI Listing
August 2013

Arg399Gln polymorphism of X-ray repair cross-complementing group 1 gene is associated with angiographically documented coronary artery disease in South Indian type 2 diabetic patients.

Genet Test Mol Biomarkers 2013 Mar 29;17(3):236-41. Epub 2013 Jan 29.

Department of Genetics, Osmania University, Hyderabad, India.

Aims: DNA damage resulting from oxidative stress contributes significantly to the development and progression of atherosclerosis in type 2 diabetic (T2DM) individuals, thereby implicating polymorphisms in DNA repair genes in the modulation of DNA repair efficiency. Based on this premise, we explored the association between X-ray repair cross-complementing group 1 (XRCC1) gene Arg399Gln polymorphism, coronary artery disease (CAD), and myocardial infarction (MI) in type 2 diabetic patients. We screened 283 T2DM patients, inclusive of 160 with angiographically defined CAD, 73 with MI, 89 without MI, and 121 T2DM individuals with no evidence of CAD for XRCC1 Arg399Gln polymorphism.

Results: There appeared to be a significant difference in the distribution of genotype and allele frequencies of XRCC1 Arg399Gln polymorphism between T2DM groups with and without CAD (p=0.03), albeit no significant association with MI was observed (p=0.055). A further analysis revealed that the frequencies of the Arg/Gln, Gln/Gln genotypes and 399Gln allele were considerably higher in patients with triple vessel disease (TVD) as compared with those with the single and double vessel disease (p=0.03), thereby associating this polymorphism with severity of CAD in T2DM individuals. Multiple logistic regression analysis revealed a significant and independent association of XRCC1 Arg399Gln polymorphism and other putative risk factors with CAD/TVD in T2DM individuals.

Conclusions: These findings reveal a significant association between XRCC1 gene Arg399Gln polymorphism, CAD/TVD, and coincident putative risk factors in T2DM individuals in the South Indian population.
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http://dx.doi.org/10.1089/gtmb.2012.0330DOI Listing
March 2013

Association of the genetic variants of endothelial nitric oxide synthase gene with angiographically defined coronary artery disease and myocardial infarction in South Indian patients with type 2 diabetes mellitus.

J Diabetes Complications 2013 May-Jun;27(3):255-61. Epub 2012 Nov 20.

Department of Genetics, Osmania University, Hyderabad, 500 007, India.

Introduction: The polymorphic variants of endothelial nitric oxide synthase (eNOS) gene have been implicated in endothelial dysfunction and are highly relevant to macroangiopathies. We investigated the relationship between eNOS gene T-786C, G894T, intron 4a/b polymorphisms and coronary artery disease (CAD) in South Indian type 2 diabetic (T2DM) individuals.

Methods: We screened 283 T2DM patients, inclusive of 160 with angiographically defined CAD, 73 with myocardial infarction (MI), 89 without MI and 121 T2DM individuals with no evidence of CAD for eNOS gene polymorphisms.

Results: There appeared to be a significant difference in the genotype and allele distribution of eNOS T-786C polymorphism between T2DM groups with and without CAD (p=0.004), albeit no significant association with MI was observed. The frequencies of TC and CC genotypes and -786C allele were considerably higher in patients with triple vessel disease (TVD) as compared to those without CAD (p=0.003), thereby associating this polymorphism with severity of CAD. Genotype and allele distributions of G894T and intron 4a/b polymorphisms were not significantly different between T2DM subjects with and without CAD/MI. Significant linkage disequilibrium was observed between intron 4a/b and T-786C polymorphisms. Multiple logistic regression analysis revealed a significant and independent association of eNOS T-786C polymorphism and other putative risk factors with CAD/TVD in T2DM individuals.

Conclusions: These findings reveal a significant association between eNOS T-786C polymorphism, CAD/TVD and coincident putative risk factors in T2DM individuals in South Indian population.
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http://dx.doi.org/10.1016/j.jdiacomp.2012.10.009DOI Listing
November 2013

Relationship between NADPH oxidase p22phox C242T, PARP-1 Val762Ala polymorphisms, angiographically verified coronary artery disease and myocardial infarction in South Indian patients with type 2 diabetes mellitus.

Thromb Res 2012 Nov 2;130(5):e259-65. Epub 2012 Oct 2.

Department of Genetics, Osmania University, Hyderabad, India.

Introduction: There has been compelling evidence for the role of oxidative stress in the pathogenesis of cardiovascular complications in type 2 diabetes mellitus (T2DM). We analyzed the association of C242T and Val762Ala polymorphisms of NADPH oxidase p22phox and poly (ADP-ribose) polymerase-1 (PARP-1) genes respectively with coronary artery disease (CAD) and its severity, myocardial infarction (MI) and cardiovascular risk factors in T2DM patients.

Materials And Methods: We screened 283 T2DM patients, inclusive of 160 with angiographically defined CAD, 73 with and 89 without MI and 121 T2DM individuals with no evidence of CAD for the two gene polymorphisms.

Results: The 242T and 762Ala alleles were significantly more frequent in T2DM subjects without CAD than those with CAD, thereby associating them with a significant protective effect against development of CAD [p=0.002 (C242T); 0.02 (Val762Ala)]. The association was further characterized by a relatively lower frequency of 242T and 762Ala alleles in T2DM patients with multi (MVD)/triple vessel disease respectively [p=0.003 (C242T); 0.02 (Val762Ala)]. Conversely, the genotype and allele frequencies of these polymorphisms were not significantly different in T2DM+CAD patients with or without MI. Stratification of risk by putative risk factors for CAD revealed a significant interaction with these polymorphisms. Multiple logistic regression analysis revealed a significant and independent association of C242T and Val762Ala polymorphisms and other putative risk factors with CAD/MVD in T2DM individuals.

Conclusions: Our observations indicate a significant relationship between p22phox C242T and PARP-1 Val762Ala polymorphisms, CAD and its severity, but not with occurrence of MI in T2DM individuals with significant coronary stenoses.
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http://dx.doi.org/10.1016/j.thromres.2012.09.012DOI Listing
November 2012

Relationship between angiotensin-converting enzyme gene insertion/deletion polymorphism, angiographically defined coronary artery disease and myocardial infarction in patients with type 2 diabetes mellitus.

J Renin Angiotensin Aldosterone Syst 2012 Dec 7;13(4):478-86. Epub 2012 Jun 7.

Department of Genetics, Osmania University, India. Hyderabad , India.

Introduction: The insertion/deletion (I/D) polymorphism of the angiotensin-converting enzyme (ACE) gene has been implicated in the pathogenesis of cardiovascular diseases. The objective of the present study was to investigate the influence of ACE gene I/D polymorphism on the development and progression of coronary artery disease (CAD) and myocardial infarction (MI) in type 2 diabetic (T2DM) patients.

Materials And Methods: We screened 283 T2DM patients, inclusive of 160 patients with angiographically defined CAD, 73 patients with MI, 89 patients without MI and 121 T2DM individuals with no evidence of CAD for ACE gene I /D polymorphism.

Results: There was no significant difference in the distribution of genotypes and alleles of ACE gene I/D polymorphism between T2DM+CAD and T2DM (non-CAD) groups. However, a significant association of this polymorphism with MI in T2DM+CAD patients (p=0.024) was observed. Further analysis revealed that the frequencies of the DD and ID genotypes increased with the number of stenosed coronary vessels (p=0.026). The DD genotype and the D allele were more frequent in the subgroup of T2DM patients with multivessel CAD (p=0.01) than in individuals with single vessel stenosis.

Conclusions: These findings reveal a significant relationship between ACE gene I/D polymorphism, multivessel CAD and also the occurrence of MI in T2DM individuals with significant coronary stenoses in our population.
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http://dx.doi.org/10.1177/1470320312448947DOI Listing
December 2012

Analytical method development and validation of prasugrel in bulk and its pharmaceutical formulation using the RP-HPLC method.

Pharm Methods 2011 Jul;2(3):173-7

Department of Pharmaceutical Analysis, SSJ College of Pharmacy, Gandipet, Hyderabad, India.

Purpose: This study was designed to develop and validate a simple, sensitive, precise, and specific reverse phase high-performance liquid chromatographic (HPLC) method for the determination of prasugrel in bulk and its tablet dosage forms.

Materials And Methods: The HPLC separation was carried out by reverse phase chromatography on an inertsil ODS-3V column (5 μm; 250 × 4.6mm(2)) with a mobile phase composed of 0.02 M potassium dihydrogen orthophosphate, 0.02 M dipotassium hydrogen orthophosphate in water:acetonitrile (30:70 v/v) in isocratic mode at a flow rate of 1 ml/min. The detection was monitored at 210 nm.

Results: The calibration curve for prasugrel was linear from 100 to 600 μg/ml. The inter-day and intra-day precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility, and specificity for the determination of prasugrel in bulk and its tablet dosage forms. The limit of detection and limit of quantification for prasugrel were found to be 0.25 μg/ml and 0.75 μg /ml, respectively. Accuracy (recoveries: 99.8-101.2%) and reproducibility were found to be satisfactory.

Conclusion: The proposed method is simple, fast, accurate, and precise for the simultaneous quantification of prasugrel in the dosage form, bulk drugs as well as for routine analysis in quality control.
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http://dx.doi.org/10.4103/2229-4708.90357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658055PMC
July 2011

Angiotensin converting enzyme (ACE) gene polymorphism in vitiligo: protective and predisposing effects of genotypes in disease susceptibility and progression.

Eur J Dermatol 2011 Mar-Apr;21(2):173-7

Osmania University, Dept. of Genetics, Hyderabad, Andhra Pradesh, Hyd -12, India.

Vitiligo is a depigmenting skin disorder with profound heterogenity in its aetio-pathophysiology, and is associated with inter-individual variation in progression of disease. Angiotensin converting enzyme (ACE) is a regulator of renin angiotensin system (RAS) that plays an important role in the physiology of the vasculature, blood pressure, inflammation, adipocyte distribution of various diseases. The present study was carried out in 243 vitiligo patients (132 males and 111 females), aged between 3-62 years with a mean age at onset of 21.6  ±  13.6 yrs, and in 205 healthy controls of south Indian origin. The main objectives of the present study were to evaluate the ACE I/D (insertion/deletion) polymorphism in the patient and control groups. Further, I/D genotypes were compared among the patients with and without the family history of vitiligo as well as the progression of the disease, through polymerase chain reaction (PCR) methods.

The results revealed a highly significant association of DD genotype with disease susceptibility (p < 0.01) in patients with a family history of vitiligo (p < 0.05) in terms of early age at onset. Further, the pre-dominance of ID genotype among patients revealed its association with a slow progression of the disease (p < 0.05). The present study is the first report to highlight the protective role of II genotype and the significant association of ID genotype with slow progression of the disease.

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http://dx.doi.org/10.1684/ejd.2011.1279DOI Listing
July 2011
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