Publications by authors named "Mohammed H Alqarni"

15 Publications

  • Page 1 of 1

Solubility Determination, Hansen Solubility Parameters and Thermodynamic Evaluation of Thymoquinone in (Isopropanol + Water) Compositions.

Molecules 2021 May 26;26(11). Epub 2021 May 26.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

This article studies the solubility, Hansen solubility parameters (HSPs), and thermodynamic behavior of a naturally-derived bioactive thymoquinone (TQ) in different binary combinations of isopropanol (IPA) and water (HO). The mole fraction solubilities () of TQ in various (IPA + HO) compositions are measured at 298.2-318.2 K and 0.1 MPa. The HSPs of TQ, neat IPA, neat HO, and binary (IPA + HO) compositions free of TQ are also determined. The data of TQ are regressed by van't Hoff, Apelblat, Yalkowsky-Roseman, Buchowski-Ksiazczak , Jouyban-Acree, and Jouyban-Acree-van't Hoff models. The maximum and minimum values of TQ are recorded in neat IPA (7.63 × 10 at 318.2 K) and neat HO (8.25 × 10 at 298.2 K), respectively. The solubility of TQ is recorded as increasing with the rise in temperature and IPA mass fraction in all (IPA + HO) mixtures, including pure IPA and pure HO. The HSP of TQ is similar to that of pure IPA, suggesting the great potential of IPA in TQ solubilization. The maximum molecular solute-solvent interactions are found in TQ-IPA compared to TQ-HO. A thermodynamic study indicates an endothermic and entropy-driven dissolution of TQ in all (IPA + HO) mixtures, including pure IPA and pure HO.
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http://dx.doi.org/10.3390/molecules26113195DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199087PMC
May 2021

Solubilization of -Resveratrol in Some Mono-Solvents and Various Propylene Glycol + Water Mixtures.

Molecules 2021 May 21;26(11). Epub 2021 May 21.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

This research deals with the determination of solubility, Hansen solubility parameters, dissolution properties, enthalpy-entropy compensation, and computational modeling of a naturally-derived bioactive compound -resveratrol (TRV) in water, methanol, ethanol, n-propanol, n-butanol, propylene glycol (PG), and various PG + water mixtures. The solubility of TRV in six different mono-solvents and various PG + water mixtures was determined at 298.2-318.2 K and 0.1 MPa. The measured experimental solubility values of TRV were regressed using six different computational/theoretical models, including van't Hoff, Apelblat, Buchowski-Ksiazczak λh, Yalkowsly-Roseman, Jouyban-Acree, and van't Hoff-Jouyban-Acree models, with average uncertainties of less than 3.0%. The maxima of TRV solubility in mole fraction was obtained in neat PG (2.62 × 10) at 318.2 K. However, the minima of TRV solubility in the mole fraction was recorded in neat water (3.12 × 10) at 298.2 K. Thermodynamic calculation of TRV dissolution properties suggested an endothermic and entropy-driven dissolution of TRV in all studied mono-solvents and various PG + water mixtures. Solvation behavior evaluation indicated an enthalpy-driven mechanism as the main mechanism for TRV solvation. Based on these data and observations, PG has been chosen as the best mono-solvent for TRV solubilization.
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http://dx.doi.org/10.3390/molecules26113091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8196874PMC
May 2021

Anti-Arthritic and Anti-Inflammatory Potential of Extract Fractions: An In Silico, In Vitro and In Vivo Approach.

Plants (Basel) 2021 Apr 21;10(5). Epub 2021 Apr 21.

Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.

The fruits of have traditionally been used for the management of rheumatism in the northeast region of India. The present study explores the probable anti-arthritis and anti-inflammatory potential of fruit extract's ethanolic fraction (EtoH-F). To support this study, we first approached the parameters in silico by means of the active constituents of the plant (beta amyrin, beta sitosterol, oleonolic acid and co-crystallised ligands, i.e., SPD-304) via molecular docking on COX-1, COX-2 and TNF-α. Thereafter, the absorption, distribution, metabolism, excretion and toxicity properties were also determined, and finally experimental activity was performed in vitro and in vivo. The in vitro activities of the plant extract fractions were evaluated by means of parameters like 1,1-Diphenyl-2- picrylhydrazyl (DPPH), free radical-reducing potential, albumin denaturation, and protease inhibitory activity. The in vivo activity was evaluated using parameters like COX, TNF-α and IL-6 inhibition assay and arthritis score in Freund Adjuvant (CFA) models at a dose of 400 mg/kg b.w. per day of different fractions (hexane, chloroform, alcoholic). The molecular docking assay was performed on COX-1, COX-2 and TNF-α. The results of in vitro studies showed concentration-dependent reduction in albumin denaturation, protease inhibitors and scavenging activity at 500 µg/mL. Administration of the alcoholic fraction at the abovementioned dose resulted in a significant reduction ( < 0.01) in arthritis score, paw diameters, TNF-α, IL-6 as compared to diseased animals. The docking results showed that residues show a critical binding affinity with TNF-α and act as the TNF-α antagonist. The alcoholic fraction of extract possesses beneficial effects on rheumatoid arthritis as well as anti-inflammatory potential, and can further can be used as a possible agent for novel target-based therapies for the management of arthritis.
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http://dx.doi.org/10.3390/plants10050825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143105PMC
April 2021

Simultaneous Estimation of Cinnamaldehyde and Eugenol in Essential Oils and Traditional and Ultrasound-Assisted Extracts of Different Species of Cinnamon Using a Sustainable/Green HPTLC Technique.

Molecules 2021 Apr 3;26(7). Epub 2021 Apr 3.

Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.

A wide range of analytical techniques are reported for the determination of cinnamaldehyde (CCHO) and eugenol (EOH) in plant extracts and herbal formulations either alone or in combination. Nevertheless, sustainable/green analytical techniques for the estimation of CCHO and EOH either alone or in combination are scarce in the literature. Accordingly, the present research was carried out to establish a rapid, highly sensitive, and sustainable high-performance thin-layer chromatography (HPTLC) technique for the simultaneous estimation of CCHO and EOH in the traditional and ultrasound-assisted methanolic extracts of , and and their essential oils. The simultaneous estimation of CCHO and EOH was performed through NP-18 silica gel 60 F254S HPTLC plates. The cyclohexane/ethyl acetate (90:10, ) solvent system was optimized as the mobile phase for the simultaneous estimation of CCHO and EOH. The greenness score of the HPTLC technique was predicted using AGREE software. The entire analysis was carried out at a detection wavelength of 296 nm for CCHO and EOH. The sustainable HPTLC technique was observed as linear in the range 10-2000 ng band for CCHO and EOH. The proposed technique was found to be highly sensitive, rapid, accurate, precise, and robust for the simultaneous estimation of CCHO and EOH. The content of CCHO in traditional methanolic extracts of , and was found to be 96.36, 118.49, and 114.18 mg g, respectively. However, the content of CCHO in ultrasound-assisted methanolic extracts of , and was found to be 111.57, 134.39, and 129.07 mg g, respectively. The content of CCHO in essential oils of , and was found to be 191.20, 214.24, and 202.09 mg g, respectively. The content of EOH in traditional methanolic extracts of , and was found to be 73.38, 165.41, and 109.10 mg g, respectively. However, the content of EOH in ultrasound-assisted methanolic extracts of , and was found to be 87.20, 218.09, and 121.85 mg g, respectively. The content of EOH in essential oils of , and was found to be 61.26, 79.21, and 69.02 mg g, respectively. The amounts of CCHO and EOH were found to be significantly higher in ultrasound-assisted extracts of all species compared to its traditional extraction and hence ultrasound extraction has been proposed as a superior technique for the extraction of CCHO and EOH. The AGREE analytical score of the present analytical technique was predicted as 0.75, suggesting excellent greenness profile of the proposed analytical technique. Based on all these observations and results, the proposed sustainable HPTLC technique can be successfully used for the simultaneous estimation of CCHO and EOH in different plant extracts and herbal products.
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http://dx.doi.org/10.3390/molecules26072054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038348PMC
April 2021

Combining Normal/Reversed-Phase HPTLC with Univariate Calibration for the Piperine Quantification with Traditional and Ultrasound-Assisted Extracts of Various Food Spices of L. under Green Analytical Chemistry Viewpoint.

Molecules 2021 Jan 31;26(3). Epub 2021 Jan 31.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

Due to unavailability of sustainable analytical techniques for the quantitation of piperine (PPN) in food and pharmaceutical samples, there was a need to develop a rapid and sensitive sustainable analytical technique for the quantitation of PPN. Therefore, the current research presents a fast and highly sensitive normal/reversed-phase high-performance thin-layer chromatography (HPTLC) technique with classical univariate calibration for the quantitation of PPN in various food spices of black pepper with traditional (TE) and ultrasound-assisted extracts (UBE) of various food spices of L. under green analytical chemistry viewpoint. The amount of PPN in TE of four different spices of black pepper-namely BPMH, BPLU, BPSH, and BPPA-was found to be 309.53, 304.97, 282.82, and 232.73 mg g, respectively using a sustainable normal-phase HPTLC technique. However, the amount of PPN in UBE of BPMH, BPLU, BPSH, and BPPA was recorded as 318.52, 314.60, 292.41, and 241.82 mg g, respectively using a sustainable normal phase HPTLC technique. The greenness of normal/reversed-phase HPTLC technique was predicted using AGREE metric approach. The eco-scale was found to be 0.90, suggested excellent greenness of normal/reversed-phase technique. UBE of PPN was also found to be superior over TE of PPN. Overall, the results of this research suggested that the proposed normal/reversed-phase densitometry technique could be effectively used for the quantitation of PPN in food and pharmaceutical samples.
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http://dx.doi.org/10.3390/molecules26030732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866824PMC
January 2021

Quantitative Analysis of Emtricitabine in Dosage Forms Using Green RP-HPTLC and Routine NP-HPTLC Methods-A Contrast of Validation Parameters.

ACS Omega 2020 Dec 18;5(51):33470-33477. Epub 2020 Dec 18.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

In this research, an antiviral drug emtricitabine (ECT) was quantified using the validated green reversed-phase high-performance thin-layer chromatography (RP-HPTLC) and routine normal-phase HPTLC (NP-HPTLC) methods in the marketed oral solutions and capsules. Green RP-HPTLC-densitometry quantification was performed using the acetone/water (70:30, v/v) solvent system as the mobile phase. Routine NP-HPTLC-densitometry quantification was performed using the chloroform/methanol (85:15, v/v) solvent system as the mobile phase. The detection was performed at λ-285 nm for both of the methods. Both densitometry methods were validated for different parameters. Most of the validation parameters including linearity, precision, accuracy, detection, and quantification limits for the green densitometry method were found to be superior compared to the routine densitometry technique. The ECT contents of commercial oral solution and commercial capsules were found to be 100.85 and 98.27%, respectively, using the green densitometry technique. The ECT contents of oral solutions and capsules were 97.16 and 95.54%, respectively, using the routine densitometry technique. Accordingly, the green densitometry technique was found to be better than the routine densitometry technique for ECT assays. Thus, the green densitometry technique can be successfully applied for the quantitation of ECT in the marketed formulations.
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http://dx.doi.org/10.1021/acsomega.0c05537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774264PMC
December 2020

Anti obesity prospective of (Roxb.) hydroalcoholic bark extract in high fat diet induced obese rats.

3 Biotech 2020 Nov 27;10(11):493. Epub 2020 Oct 27.

Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box No. 173, Al-Kharj, 11942 Kingdom of Saudi Arabia.

Obesity is an alarming sign and considered as a threat word wide. Since it not only hurt the human body but plays as a basis for other serious diseases like cardiovascular and many more. The 50% hydro-ethanolic extract of bark () (DLBE; %yield = 16.34) and methanolic extract (4.32%) of were made. The DLBE was used for the acute oral toxicity and anti-obesity activity in the rodent. However, methanolic extract was used for characterization by high-performance thin layer chromatography (HPTLC) method. During acute toxicity study, it was shown that certainly there was no mortality or morbidity observed up to the maximum dose of 2000 mg/kg after administration of DLBE. The ultimate body weight, food intake, liver weight, total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), triglycerides (TG), aspartate aminotransferase (AST), alanine transaminase (ALT), serum creatinine and blood urea nitrogen (BUN) of rats treated with DLBE at a dose of 200 and 400 mg/kg respectively was considerably diminished to  < 0.01 and  <  as compared with high-fat diet (HFD) induced obese animals. However, DLBE treated with quite smaller dose revealed a non-significant ( > 0.05) effects on above parameters. The histopathological findings of the study from the cross section of liver and kidney show normal architecture in the cells treated with DLBE at a dose of 200 and 400 mg/kg respectively. Thus we can conclude that the bark extract of can be used for the treatment of obesity and a novel approach for further investigations of its pathology.
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http://dx.doi.org/10.1007/s13205-020-02491-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591644PMC
November 2020

Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study.

Antibiotics (Basel) 2020 Jun 25;9(6). Epub 2020 Jun 25.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using "RP-18 silica gel 60 F254S HPTLC plates". However; NP-HPTLC estimation of DLFX was performed using "silica gel 60 F254S HPTLC plates". For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 //) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 //) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.
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http://dx.doi.org/10.3390/antibiotics9060359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344820PMC
June 2020

Simple and Accurate HPTLC-Densitometric Method for Quantification of Delafloxacin (A Novel Fluoroquinolone Antibiotic) in Plasma Samples: Application to Pharmacokinetic Study in Rats.

Antibiotics (Basel) 2020 Mar 23;9(3). Epub 2020 Mar 23.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

Delafloxacin (DLX) is a recently-approved fluoroquinolone antibiotic, which is recommended for the treatment of "acute bacterial skin and skin structure infections". A thorough literature survey revealed only a single published method for the estimation of DLX using UPLC-MS/MS technique in biological samples. There is no high-performance thin-layer chromatography (HPTLC) method has been reported for the estimation of DLX in dosage forms and/or biological samples. Therefore, a selective, sensitive, rapid and validated HPTLC-densitometry technique has been used for the estimation of DLX in human plasma for the first time. HPTLC quantification of DLX and internal standard (IS; gatifloxacin) was carried out on glass coated silica gel 60 F HPTLC plates using the ternary mixture of ethyl acetate:methanol:ammonia solution 5:4:2 (%, ) as the mobile phase. Densitometric detection was done at 344 nm. The R values were recorded as 0.43 and 0.27 for the DLX and the IS, respectively. The linearity range of DLX was obtained as 16-400 ng/band. A simple protein precipitation method was used for the extraction of analyte from plasma using methanol. The proposed HPTLC technique was validated for "linearity, accuracy, precision, and robustness". The proposed HPTLC technique was successfully utilized for the assessment of pharmacokinetic profile of DLX in rats after oral administration. After oral administration, the peak plasma concentration of DLX was obtained as 194.19 ng/ml in 1 h. The proposed HPTLC method could be applied in study of pharmacokinetic profile and therapeutic drug monitoring of DLX in clinical practice.
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http://dx.doi.org/10.3390/antibiotics9030134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175235PMC
March 2020

Chemical composition and protective effect of L. essential oil against CCl induced hepatotoxicity.

Saudi Pharm J 2019 Nov 15;27(7):945-951. Epub 2019 Jul 15.

Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.

The hepatoprotective activity of the total extract of L. against CCl induced toxicity in experimental animals was previously reported and indicated promising results. Essential oil of was prepared by hydrodistillation method. Components of the oil were identified by comparison of GC-MS and retention indexes with reported data. The hepatoprotective effect of the essential oil against CCl induced toxicity was studied using male Wistar rats and silymarin at 10 mg/kg p.o as standard drug. The protective effect was evaluated via serum biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), and total bilirubin as well as tissue parameters including non-protein sulfhydryl groups (NP-SH), malonaldehyde (MDA) and total protein (TP). Histopathological study was applied on the liver tissues using Mayer's hematoxylin stain, Periodic Acid Schiff - Hematoxylin (PAS-H) and Masson trichrome technique on light microscope. Electron microscope images were also obtained for more detailed study.
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http://dx.doi.org/10.1016/j.jsps.2019.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978616PMC
November 2019

Characterization and hepatoprotective evaluation of sesquiterpenes and diterpenes from the aerial parts of L.

Saudi Pharm J 2019 Nov 22;27(7):920-929. Epub 2019 Jun 22.

Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.

Previously, we reported on the hepatoprotective activity of the total extract of L. against CCl induced liver toxicity in experimental animals. Biologically directed phytochemical study was conducted to identify the active compounds. Male Wistar rats and the standard drug silymarin were used in the study. Hepatoprotective activity was evaluated via serum biochemical parameters such as aspartate amino transferase (AST), alanine aminotransferase (ALT), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) and total bilirubin. Tissue parameters including non-protein sulfhydryl groups (NP-SH), malonaldehyde (MDA) and total protein (TP) were also determined. Histopathological study was conducted utilizing Mayer's hematoxylin stain, Periodic Acid Schiff - Hematoxylin (PAS-H) and Masson trichrome technique on light microscope. Electron microscope images were also generated for the study. The activity of the total extract was trapped to the petroleum ether fraction after liquid-liquid fractionation where 51% reduction in the levels of AST, bilirubin and 44% in the levels of ALT were observed. Chromatographic purification of the petroleum ether fraction resulted in the isolation of nine compounds namely: -calamenene , cadalene (cadalin) , -cubenol , manool , calamenene-10-ol , calamenene-10-ol , 4--abietic acid , sandaracopimaric acid and isopimaric acid . Compounds -, and are belonging to cadinane sesquiterepenes, while compounds 4, 7-9 were of diterpene skeleton. The major compounds were tested for their hepatoprotective effect. Compounds showed marked improvement in the levels of AST and ALT, compound was effective in improving the levels of AST, ALT, GGT, ALP and bilirubin, while compound showed significant improvement in GGT, ALP and bilirubin levels.
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http://dx.doi.org/10.1016/j.jsps.2019.06.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978623PMC
November 2019

Chemical composition of fennel seed extract and determination of fenchone in commercial formulations by GC-MS method.

J Food Sci Technol 2019 May 10;56(5):2395-2403. Epub 2019 Apr 10.

4Kayyali Chair for Pharmaceutical Industries, Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451 Saudi Arabia.

In the present study, various phytoconstituents of methanolic extract of were identified using gas-chromatography mass spectrometry (GC-MS) method. GC-MS method was also applied for the analysis of biomarker fenchone in extract and eight different commercial formulations. The mass of prepared extract and formulations A-D and H (commercial herbal mixtures and commercial extract) used for the analysis of fenchone was 10 g. However, the mass of formulations E-G (soft gelatin capsules) was 100 mg. Fifty seven different phytoconstituents were identified in the methanolic extract of using GC-MS technique. The main compounds identified were trans-anethole (31.49%), 2-pentanone (25.01%), fenchone (11.68%) and benzaldehyde-4-methoxy (8.01%). Several other compounds were also identified in higher amounts and some compounds were identified in trace amounts. Many compounds have been reported for the first time in the methanolic extract of . The amount of fenchone was found to be maximum in plant extract (9.789 mg/g) in comparison with other commercial formulations by the proposed GC-MS technique. In three different commercial formulations (F, G and H), the amount of fenchone was obtained as more than 1.0 mg/g. However, in five different commercial formulations (A, B, C, D and E), the amount of fenchone was recorded as less than 0.1 mg/g. This method could be utilized for the analysis of fenchone contents in the commercial formulations containing fenchone as an active ingredient. The results obtained in this work could be useful in standardization of commercial formulations containing fenchone.
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http://dx.doi.org/10.1007/s13197-019-03695-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525722PMC
May 2019

Wound healing evaluation of self-nanoemulsifying drug delivery system containing essential oil.

3 Biotech 2019 Mar 14;9(3):82. Epub 2019 Feb 14.

2Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj, 11942 Saudi Arabia.

The objective of this work was to characterize and evaluate wound healing potential of oil (PO) via self-nanoemulsifying drug delivery system (SNEDDS) in comparison with standard gentamycin. PO loaded SNEDDS was prepared by low energy emulsification technique and characterized for thermodynamic stability, self-emulsification power and various physico-chemical parameters. An optimal formula of PO SNEDDS was subjected to wound healing evaluation, collagen determination and histomorphological examination in female Wistar rats as compared with pure PO and standard antibiotic/gentamycin. An optimal formula of PO SNEDDS showed significant wound healing effects in Wistar female rats in comparison with pure PO. However, wound healing effects of optimized SNEDDS were comparable with standard gentamycin. An optimized formulation also indicated significant enhancement in collagen content (0.82 mg/g) in comparison with pure PO (0.53 mg/g) and negative control (0.33 mg/g). While, the collagen content of SNEDDS (0.82 mg/g) treated rats were comparable with standard gentamycin treated animals (0.98 mg/g). Histopathological examinations of optimized SNEDDS treated animals showed no signs of inflammatory cells which indicated that prepared SNEDDS was safe and nontoxic to rats. The results obtained in this work showed the potential application of SNEDDS in enhancement of the wound healing activity of PO upon oral administration.
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http://dx.doi.org/10.1007/s13205-019-1630-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375821PMC
March 2019

Effect of hydroxylated solvents on the active constituents of Salvadora persica root "Siwak".

Saudi Pharm J 2019 Feb 8;27(2):220-224. Epub 2018 Nov 8.

Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.

Previously, the antimicrobial activity of was traced to benzyl isothiocyanate. In the present study known inactive compounds were isolated from extracts obtained by different solvents including -amyrin, -sitosterol, stigmasterol glucoside, benzyl cyanide and sulphur. However, some inactive compounds were present only in the ethanol and methanol extracts. This observation indicated that these compounds most likely are artifacts resulted from interaction with the solvents used in extraction. Pure benzyl isothiocyanate was kept with different solvents for 72 h and after TLC study they were heated under reflux for 8 h to explore the possibility of interactions. Only solvents with OH groups reacted with benzyl isothiocyanate and gave products similar to those isolated from the alcohol extracts. In conclusion extraction of with hydroxylated solvents will alter the structure of the active compound benzyl isothiocyanate and leads to loss of antimicrobial activity.
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http://dx.doi.org/10.1016/j.jsps.2018.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362162PMC
February 2019

Wound Healing Study of Eucalyptus Essential Oil Containing Nanoemulsion in Rat Model.

J Oleo Sci 2018 Aug 17;67(8):957-968. Epub 2018 Jul 17.

Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University.

The objective of this investigation was to develop nanoemulsion formulations of Eucalyptus essential oil (EEO) and to evaluate its wound healing effects in comparison with standard gentamycin in rat model. Various nanoemulsionns of EEO were prepared using aqueous phase titration method and the zones of nanoemulsion were identified by the construction of phase diagrams. EEO nanoemulsions were investigated in terms of physical stability, self-nanoemulsification efficiency and physicochemical characterization. Optimized nanoemulsion of EEO was selected for wound healing study, collagen estimation and histopathological evaluation in rats in comparison with pure EEO and standard gentamycin. Optimized nanoemulsion presented significant would healing activity in rats as compared with pure EEO upon oral administration. The wound healing activity of optimized nanoemulsion was comparable with standard gentamycin. Optimized EEO nanoemulsion also presented significant enhancement in collagen content as compared with pure EEO and negative control. However, the collagen contents of optimized nanoemulsion treated animals were comparable with standard gentamycin-treated animals. Histopathological studies of optimized nanoemulsion treated rats showed no signs of inflammatory cells which suggested the safety and non-toxicity of EEO nanoemulsion. This study suggested the potential of nanoemulsion in enhancing the wound healing activity of EEO upon oral administration.
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http://dx.doi.org/10.5650/jos.ess18005DOI Listing
August 2018