Publications by authors named "Mohammadreza Gholami"

33 Publications

A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice.

Oxid Med Cell Longev 2020 23;2020:9132724. Epub 2020 Jun 23.

Department of Anatomy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: Both antioxidant and prooxidant activities have been previously reported for cerium oxide (CeO). The aim of this study was to investigate the effects of CeO at different doses on changes in kidney tissues and markers in neonatal mice.

Methods: We randomly divided 30 pregnant NMRI mice into five groups ( = 6 per group)-a control group and four groups treated with intraperitoneal (i.p.) administration of different doses of CeO (10, 25, 80, or 250 mg/kg body weight (bw)) on gestation days (GD) 7 and GD14. At the end of the treatment period, we analyzed the kidney tissues and serum samples. The levels of two serum redox markers, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP), were determined. Data were analyzed using one-way ANOVA and Tukey's test, and a value of <0.05 was considered significant.

Results: The mean total volumes of the renal corpuscle, glomeruli, and Bowman's capsule membranes significantly increased, and there was a significant decrease in the mean total volume of Bowman's space in the high-dose CeO group compared to that in the control group. No statistically significant differences existed in the serum levels of MDA and FRAP in the treated and control groups.

Conclusion: Our results suggest that high doses of CeO impair fetal renal development in pregnant mice, which results in kidney damage. Therefore, CeO administration during pregnancy could have dose-dependent adverse effects on the developing kidneys in neonates.
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http://dx.doi.org/10.1155/2020/9132724DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7330649PMC
June 2020

Protective effects of royal jelly on testicular torsion induced ischaemia reperfusion injury in rats.

Andrologia 2020 Oct 22;52(9):e13716. Epub 2020 Jun 22.

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

This study was performed to investigate the protective effects of royal jelly (RJ) on a testicular torsion-induced ischaemia/reperfusion (I/R) injury in adult rats. A total of 40 male Wistar rats were divided into four groups, including 10 rats in each group: Group 1 (sham), Group 2 (Control), group 3 (I/R rats treated with 100 mg/kg RJ for 50 days after torsion) and group 4( I/R rats treated with 20 mg/kg vitamin C for 50 days after torsion). Testicular torsion was created by rotating the right testes 720° a clockwise direction for 90 min. The levels of testosterone were measured by ELISA. Pathological evaluation, mean maturity and quality of the seminiferous tubules were used. Results showed that the testicular histopathology standards and testosterone levels changes were statistically significant in groups 3 and 4. The results obtained in this study may suggest that RJ like vitamin C had protective effects on a testicular ischaemia/reperfusion-induced injury in rats.
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http://dx.doi.org/10.1111/and.13716DOI Listing
October 2020

Quercetin postconditioning attenuates gastrocnemius muscle ischemia/reperfusion injury in rats.

J Cell Physiol 2020 12 21;235(12):9876-9883. Epub 2020 May 21.

Department of Anatomical Sciences, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Quercetin, an antioxidant derived from plants, can play a beneficial role in the protection of various tissues against ischemia-reperfusion injuries (IRI). The purpose of the present research was to investigate the protective effects of quercetin on gastrocnemius muscle ischemia-reperfusion. A total of 80 adult male Wistar rats (weights: 250-300 g) were divided into ten groups (n = 8 per group). We used silk 6.0 surgical thread to create a knit to occlude the femoral artery and vein for 3 hr. The treated groups, which comprised half of each experimental group, received intraperitoneal injections of 150 mg/kg quercetin after the ischemia. Blood flow was subsequently reestablished in the reperfusion phase. The rats were kept in reperfusion for 3, 7, 14, or 28 days after which they were killed with high doses of anesthetic drugs, and the gastrocnemius muscles were removed and fixed. Tissue processing, hematoxylin and eosin and toluidine blue staining, and immunohistochemistry were used to assess tumor necrosis factor-α (TNF-α) and nuclear factor κB (NF-κB) levels. A comparison between treated and untreated ischemic sites showed that on the third day of reperfusion, the severity of edema and NF-κB level decreased significantly; on the 7th day of reperfusion, the severity of edema and the levels of TNF-α and NF-κB decreased significantly; and on the 14th day of reperfusion, all of the parameters showed significant decreases. On the 28th day of reperfusion, there were significantly decreased levels of TNF-α and NF-κB, and decreased mast cell infiltration when compared with the untreated groups. According to the results, administration of quercetin after ischemia could significantly prevent gastrocnemius muscle IRI.
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http://dx.doi.org/10.1002/jcp.29801DOI Listing
December 2020

Pre-Implantation Effects of Progesterone Administration on Ovarian Angiogenesis after Ovarian Stimulation: A Histological, Hormonal, and Molecular Analysis.

JBRA Assist Reprod 2020 Jul 14;24(3):289-295. Epub 2020 Jul 14.

Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.

Objective: Progesterone (P4) is known to directly affect ovarian tissue angiogenesis. The present study was designed to show how P4 affects ovarian angiogenesis in hormonal, histological, and molecular levels.

Methods: Fifteen adult female NMRI mice were divided into three groups: Control Group; Case Group I (ovarian stimulation alone); and Case Group II (ovarian stimulation followed by P4 administration). Blood and ovarian tissue samples were assessed for hormonal, histological, and molecular alterations. Gene expression for ovarian vascular endothelium growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1α) was analyzed using real-time PCR.

Results: Ovarian hormone levels were increased in the case groups compared with the control group (p<0.05). Quantitative corpus luteum parameters were increased in the case groups compared with the control group (p<0.05). Quantitative ovarian vascular parameters were significantly different in the case groups compared with the control group. Gene expression analyses shows that the mice in Case Group I had higher levels of ovarian VEGF expression than the mice in the control group (p<0.05). No significant difference in gene expression was observed for HIF-1ɑ.

Conclusion: Treatment with P4 after ovarian stimulation enhanced ovarian angiogenesis by increasing hormone levels and causing significant structural changes.
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http://dx.doi.org/10.5935/1518-0557.20190076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365533PMC
July 2020

Effect of selenium on freezing-thawing damage of mice spermatogonial stem cell: a model to preserve fertility in childhood cancers.

Stem Cell Investig 2019 26;6:36. Epub 2019 Nov 26.

Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: During treatment of childhood cancers, fertility of boys may be affected. Therefore, freezing spermatogonial stem cell (SSC) is recommended. However, freezing-thawing process may cause damage to SSCs. This study was conducted to evaluate protective effects of selenium on freezing-thawing damage of mice SSCs using investigation of cell viability and investigation of apoptosis related genes expression including , , , and .

Methods: SSCs were extracted from 80 6-day-old mice. The SSCs were divided into four groups: cryopreservation along with selenium (low and high dose), vitrification along with selenium (low and high dose), cryopreservation control, and vitrification control. Trypan blue staining and real-time polymerase chain reaction (real-time PCR) were used to investigate cell viability and gene expression, respectively.

Result: Comparison of cell viability in the experimental groups did not show a significant association. Expression of and was significantly lower in cryopreservation group with low-dose selenium. Expression of was significantly lower in cryopreservation group with high-dose selenium. Expression of and was significantly lower in vitrification group with low-dose selenium, and expression of was significantly upper. Expression of and was significantly lower in vitrification group with high-dose selenium, and expression of was significantly upper (P<0.001).

Conclusions: Selenium had dose dependent effect on apoptosis related genes profile. The only evident effect was the effect of low-dose selenium in cryopreservation on inhibition of apoptosis via extrinsic pathway.
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http://dx.doi.org/10.21037/sci.2019.10.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917556PMC
November 2019

The Effect of Cerium Oxide During Pregnancy on the Development of the Testicular Tissue of Newborn NMRI Mice.

Biol Trace Elem Res 2020 May 14;195(1):196-204. Epub 2019 Aug 14.

Department of Anatomy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Cerium(IV) oxide is widely used as a catalyst in all aspects of human life and human beings are exposed to these materials. The purpose of this experimental study was to investigate the effect of CeO during pregnancy on alterations in the testis tissue and blood biochemical parameters in newborn mice. Pregnant NMRI mice were divided randomly into five groups (n = 6 for each group) including one control group and 4 treatment groups. Injection of CeO solution was administered intraperitoneally at the doses of 10, 25, 80, and 250 mg/kg.bw, respectively, on GD 7 and GD 14. At the end of treatment period, the testicular histological and biochemical parameters of 2- and 6-day-old newborns were analyzed, as well as the biochemical parameters in serum samples of 15-day-old newborns. The number of spermatogonia, Sertoli, and Leydig cells in the testis of the 2-day-old newborn and spermatogonia and Leydig cells in the testis of the 6-day-old newborns in the 250 mg/kg.bw CeO treatment group was significantly reduced compared with the control group (P < 0.05). Testis MDA of the 2- and 6-day-old newborns in the treated group receiving 250 mg/kg.bw of CeO was significantly higher than the control group (P < 0.001). There was no significant difference between serum MDA and TAC levels between the treated groups with different doses of CeO compared with the control group. Therefore, CeO given to dams during pregnancy may affect the testicular tissue and blood biochemical parameters in neonates and may be dose-dependent.
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http://dx.doi.org/10.1007/s12011-019-01836-xDOI Listing
May 2020

stimulate proliferation of mouse embryonic fibroblasts via expression of growth factors TGFβ1 and bFGF.

Inflamm Regen 2019 20;39. Epub 2019 Apr 20.

5Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.

Background: TGF-β has an important role in the process of wound healing and scar formation. The aim of this study is to determine the effects of ethanolic and methanolic extracts of on the expression of TGFβ1 and bFGF in the mouse embryonic fibroblast cells (MEFs).

Methods: extract was purchased and different substances defined with gas chromatography and mass spectrometry. MEFs were prepared and after incubating for 15 min, cell viability analyzed. TGF β 1 and bFGF gene expression was evaluated by real-time PCR. TGFβ1 and bFGF protein expression analyzed by ELISA. The statistical analysis of data was done by using SPSS software. Differences were considered significant at ( < 0.05).

Results: The results of the MTT test showed that the concentrations of 5 μg/ml and10 μg/ml were more suitable for cell proliferation. There was an increase in TGF β 1 gene expression in the MEFs. Expression of TGF β 1 gene remains the same after 24 h. Gene expression of bFGF showed a similar pattern with β expression for both solvents. Analysis of TGFβ1 protein expression showed an increase in TGFβ1 gene expression in the MEFs. Protein expression of bFGF in the MEFs increased at different concentrations at 12 and 24 h after treatment ( < 0.05 and  < 0.01 respectively).

Conclusion: stimulates proliferation of MEFs. Calendula via increased expression of growth factors (TGFβ1 and bFGF) at the first 12 h and a decrease of these factors at 24 h after treatment may ameliorate function of the MEFs in the during wound healing.
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http://dx.doi.org/10.1186/s41232-019-0097-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475102PMC
April 2019

Effect of royal jelly on testicular antioxidant enzymes activity, MDA level and spermatogenesis in rat experimental Varicocele model.

Tissue Cell 2019 Apr 20;57:70-77. Epub 2019 Feb 20.

Animal Sciences Research Institute (ASRI) of Jihad-e-Agriculture Ministry, Iran.

Varicocele is one of the most prevalent causes of infertility. It causes induction of oxidative stress, increases lipid peroxidation in the testis and disrupts spermatogenesis cycle. The aim of the study was to investigate the possible protective effects of royal jelly against varicocele induced oxidative stress, biochemical and histological alterations in the experimental varicocele model in rat. Twenty-one adult Wistar rats were divided into three groups. The control group (I), Varicocele and administration of normal saline (II), varicocele and treatment with RJ (III). At the end of the experiment, all the animals were sacrificed and testes excised. The activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and MDA levels were measured. Also, histopathological examinations, Johnsen scores and sperm parameters were determined. There was a significant (p<0.05) increase in the activity level of CAT (0.223±0.005), SOD (0.177±0.0062), GPx (9.575±0.318) and a significant (p<0.05) reduction in the MDA level (2.674±0.336) of the experimental varicocele treated with royal jelly when compared to the activity of CAT (0.011±0.004), SOD (0.035±0.0096), GPx (8.864±0.397) and MDA level (4.630±0.579) of the experimental varicocele and administration of normal saline. Results of the Johnsen score showed a significant increase (p<0.05) in the mean score of the RJ group (7.94±1.5) when compared to the normal saline group (6.04±1.4). Therefore, RJ is a potential area for further studies and improving in spermatogenesis cycle after varicocele.
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http://dx.doi.org/10.1016/j.tice.2019.02.005DOI Listing
April 2019

Encapsulation of extract in alginate hydrogel accelerate wound healing in adult male rats.

Inflamm Regen 2019 30;39. Epub 2019 Jan 30.

6Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.

Background: Finding the best dressing for a specific had continued from the past to present. The aim of this study was to evaluate the of encapsulated extract of in hydrogel alginate at wound healing.

Methods: Thirty-two male Wistar rats with a puncture wound in the back of the neck skin were divided randomly into four groups including a control group, -treated group, hydrogel alginate-treated group, and encapsulated in hydrogel alginate-treated group. Rats were treated for 22 days. The skin samples were taken on 3rd, 7th, 14th, and 22nd days after treatment for light microscopy. Results were analyzed in accordance with Kruskal-Wallis and Friedman test (for histopathology analysis) by using SPSS v.22 software.

Results: Macroscopically evaluations and measurement of wound size showed increased wound healing process in the treated groups. The complete improvement was created on the 14th day. The wound site was not observed on the 22nd day. But the wound site was observed on the 22nd day in the control group. Also, comparison of the percentage of wound healing between the treated and control groups on 3rd, 7th, 14th, and 22nd days showed a significant difference ( < 0.05). Comparison of the H&E stained sections in the studied groups showed that treated groups were effective on wound healing in comparison with the control group.

Conclusions: Encapsulated extract of in hydrogel alginate may accelerate wound improvement and increase the rate of wound healing without scar formation.
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http://dx.doi.org/10.1186/s41232-019-0090-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6352332PMC
January 2019

The Effect of Caffeic Acid on Spermatogonial Stem Cell-type A Cryopreservation.

Rep Biochem Mol Biol 2018 Oct;7(1):85-93

Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. However, during this process, the stem cells often become damaged which degrades their value for experiments and treatments. Caffeic acid (CA) is an antioxidant that has been shown to increase the viability of cells under stress. The aim of this study was to investigate the effect of CA has on spermatogonial stem cell (SSC) cryopreservation.

Methods: Spermatogonial stem cells isolated from the testes of Balb/c mice pups were cultured in laminincoated dishes, purified using CD90.1 microbeads, then cryopreserved in vitrification media supplemented with 10 µM CA either through a slow or rapid freezing process. After thawing, cell viability was evaluated. Expression of Bax, Fas, Bcl-2 and P53 genes was determined by real-time PCR. Gel electrophoresis was used to confirm the results of the real-time PCR.

Results: The viability of the SSCs that were rapidly frozen and treated with CA was observed to be significantly reduced compared to the control group (p < 0.003). The viability SSCs that received CA and underwent the slow freezing treatment was significantly reduced compared to controls (p < 0.002). The expression levels of BAX, BCL-2, and Fas in the rapid freeze-thaw group didn't significantly change. However, the levels of P53 expression were shown to increase. In the group of SSCs that underwent the slow freezing process, the BAX gene expression levels increased, while the levels of BCL-2 gene expression decreased. No significant changes in the level of Fas and P53 expression were detected. When comparing the groups that received CA treatment, SSCs that were rapidly frozen showed an up-regulation of Fas and P53 expression and a down-regulation of Bcl-2 and Bax expression.

Conclusion: Caffeic acid may protect intact SCCs during the cryopreservation process through stimulating the induction of apoptosis in injured SSCs. Supplementing the vitrification media with CA has a superior effect on the preservation of SSCs.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175585PMC
October 2018

Troxerutin protect sperm, seminiferous epithelium and pituitary-gonadal axis from torsion-detorsion injury: An experimental study.

Int J Reprod Biomed 2018 May;16(5):315-322

Department of Anatomical Sciences, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: Troxerutin is a flavonoid antioxidant that protect different organ against damage caused by ischemia-reperfusion

Objective: The aim of this study was to evaluate the effect of troxerutin in reducing the damages caused by ischemia-reperfusion in rat's testis.

Materials And Methods: 40 Male Wistar rats (2 month old) were divide to four groups (n=10). Group1 (sham), Group 2 (control, ischemia-reperfusion (I/R) without treatment), Group 3 (I/R+150 mg/kg of troxerutin), and group 4 (I/R+20 mg/kg of vitamin C). Treatment of group 3 and group 4 during torsion (twists 720 counter clock at 90 min) followed by 50 days detorsion. After 50 days, blood samples were collected and rats in all study groups were killed and their testes were removed, and fixed with Bouin's solution. Testis was stained with hematoxylin and eosin dye and the level of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were measured with ELISEA methods. TUNEL was employed to detect apoptosis. Epididymis caudal part was removed and total sperm count was determined. Johnson techniques were used for assessment of seminiferous tubules quality.

Results: Troxerutin treated group has higher Johnson score's (p≤0.001), antiapoptotic properties (p≤0.001), sperm count (p=0.065), and higher LH (p≤0.001), FSH (p≤0.001) and testosterone (p=0.002) levels than control group. Vitamin C treated group showed increase level of testosterone but didn't show significant differences on the number of apoptotic cells, Johnson scores, LH, FSH and sperm count than control group.

Conclusion: Troxerutin has protective effects on testicular torsion induced injury and can ameliorate spermatogenesis in the torsion-detorsion models.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6046204PMC
May 2018

Effect of ghrelin on the biochemical and histopathology parameters and spermatogenesis cycle following experimental varicocele in rat.

Andrologia 2018 Dec 13;50(10):e13106. Epub 2018 Jul 13.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Varicocele is one of the major causes of infertility in men in which testicular function is progressively damaged.

Objectives: This study aimed to determine the effect of ghrelin on antioxidant enzymes activity (catalase, SOD, GPx), malondialdehyde (MDA) level and spermatogenesis cycle after induction of varicocele in rat.

Materials And Methods: Twenty-one male Wistar rats were randomly divided into three groups: I-control group, II-rats with induced varicocele and injection of physiological saline and III-rats with induced varicocele and injection of ghrelin. At the end of the experiment, animals were sacrificed and their testes were removed. Antioxidant enzymes activity and MDA level were measured. Histopathological tests, Johnsen's score and sperm parameters were also evaluated.

Results: In varicocele group with ghrelin administration (group III), the levels of SOD (0.183 ± 0.024), GPX (9.4250 ± 0.103) and TAC (2.79 ± 0.464) increased significantly (p < 0.05), while MDA (0.304 ± 0.004) level decreased significantly (p < 0.05) compared with varicocele and normal saline group (II). There was no significant difference in the activity of catalase between group III (0.122 ± 0.018) and group II (0.108 ± 0.018), although ghrelin improved catalase activity in group III compared to group II. Also, in group III, there were significant increases in the Johnsen's score (7.920), sperm count (70.29 ± 5.82) and sperm viability (87.14 ± 5.21) compared with group II (p < 0.05).

Conclusion: Ghrelin can improve the capacity of antioxidant enzymes to reduce the oxidative stress caused by varicocele and reduce spermatogenesis cycle. Therefore, special attention should be paid to ghrelin in studies evaluating antioxidant compounds in varicocele.
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http://dx.doi.org/10.1111/and.13106DOI Listing
December 2018

Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury.

J Complement Integr Med 2018 May 23;15(4). Epub 2018 May 23.

School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Introduction The aim of the present study was to survey the protective effect of pretreatment with Persian honey on amelioration of side effects of chemotherapy and ischemia/reperfusion induced testicular injury. Materials and methods Forty adult's male wistar rats were divided into four groups of ischemia-reperfusion (IR), honey + ischemia-reperfusion (HIR), Busulfan (B) and Busulfan intraperitoneally+ honey (BH). The seminiferous tubules were rated for their modified spermatogenesis index (SI) by Johnsons score. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed using the in-situ cell death detection kit. Total serum concentration of Follicle-stimulating hormone (FSH) , Luteinizing hormone (LH) and testosterone was measured using ELISA. All data were expressed as mean ± SD and significance was set at p≤0.05. Results Honey improved SI in the HIR and BH groups and serum levels of FSH and LH in the BH and HIR groups (p<0.001). Also, serum levels of testosterone were significantly higher in BH and HIR groups. But, apoptotic cells in IR and B groups significantly increased (p<0.001), while in HIR and BH groups, the number of apoptotic cells decreased and the positive cells of TUNEL (TdT-mediated dUTP-X nick end labelling) staining were detected in spermatocytes and spermatid. Discussion Pretreatment with honey protect testis against chemotherapy and testicular IR injury, increase FSH and LH and testosterone and decrease the cellular damage and apoptosis. Honey can decrease the side effects of chemotherapy on reproductive system and prevent sterility.
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http://dx.doi.org/10.1515/jcim-2016-0035DOI Listing
May 2018

Protective effects of melatonin and ghrelin on spermatogenesis: A narrative review of the literature.

Int J Reprod Biomed 2017 May;15(5):265-272

Department of Veterinary Pathology, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

Spermatocytogenesis starts from lumens of seminiferous cords and after migration to the basal membrane ends to the lumens again. We attempt to review the protective effects of melatonin and ghrelin on Spermatocytogenesis and in particular on spermatogonial stem cells, as two rather newly-discovered hormones. Testicular freezing prior to chemotherapy and radiotherapy is one of the ways of preserving fertility in children with cancer. The freezing has two methods of slow-freezing (cryopreservation) and rapid-freezing (vitrification). Administration of melatonin can maintain the quality of the germ cells underwent such processes, as well as ghrelin, can protect germ cells from the toxicities secondary to ischemic injuries, and pathologic apoptosis. This review indicates that in vitro or in vivo administration of melatonin or ghrelin, could be effective to preserve fertilization and also they can be used in assisted reproductive technologies to improve the quality of sperms. Future original studies should be propelled toward human studies, of course with observing the ethics.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5510579PMC
May 2017

Effects of pomegranate peel extract on histopathology, testosterone levels and sperm of testicular torsion-detorsion induced in adult Wistar rats.

J Complement Integr Med 2017 Jul 22;14(4). Epub 2017 Jul 22.

.

Background In the present study, effects of pomegranate peel extract have been evaluated on decreasing the damage induced by testis torsion. Methods In this study, 30 adult Wistar rats were randomly divided into three groups of control, experimental (1) and experimental (2).

Control: no ischemia, received vehicle alone, exposed to sham operation. Experimental (1): Received the vehicle alone during ischemia followed by 60 days' reperfusion. Experimental (2): After performing ischemia reperfusion, 500 mg/kg of pomegranate peel extract has been used for 60 days. Blood samples and sperm samples were collected. Testes were harvested and stained with haematoxylin and eosin to study the structure of seminiferous tubules. Results The statistical comparison between sperm count and their viability and testosterone hormone amount showed a significant difference between control and experimental (1) groups and control and experimental (2) groups. The results showed an improvement of morphological condition of seminiferous tubules. Conclusions Pomegranate peel extract has revealed desirable changes on the effective parameters in infertility.
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http://dx.doi.org/10.1515/jcim-2017-0009DOI Listing
July 2017

Coenzyme Q10 protects skeletal muscle from ischemia-reperfusion through the NF-kappa B pathway.

Perfusion 2017 Jul 21;32(5):372-377. Epub 2016 Dec 21.

4 School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Objective: Coenzyme Q10 (CoQ10) has antioxidant and anti-inflammatory activity. The aim of this study was to investigate the effects of CoQ10 on the inhibition of nuclear factor-kappa B (NF-κB) activation during ischemia-reperfusion (I/R) of skeletal muscle.

Methods: For ischemia induction, the animals were anesthetized and the external iliac vessels blocked for three hours. CoQ10 or vehicle was given intraperitoneally during ischemia, just before reperfusion. Four groups received 3,7,14 and 28 days' reperfusion, respectively, after the intraperitoneal injection of CoQ10 and four corresponding groups received vehicle only. After reperfusion, the gastrocnemius muscles were removed, fixed and stained for the analysis of edema and mast cell infiltration.

Results: Immuno-histochemistry staining was performed for the detection of tumor necrosis factor alpha (TNF-α) and NF-κB. CoQ10-treated groups showed a significant decrease of mast cell infiltration in the gastrocnemius muscle and edema as compared with the corresponding non-treated groups. Also, CoQ10-treated groups showed a significant TNF-α and NF-κB expression decrease when compared to the corresponding non-treated controls. The results of this study showed CoQ10 administration with ischemia decreased interstitial edema, degeneration of muscle fibers and infiltration of mast cells.

Conclusions: It seems that CoQ10 has inhibitory effects on NF-κB and TNF-α activation.
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http://dx.doi.org/10.1177/0267659116683790DOI Listing
July 2017

Evaluation of selenium on kidney function following ischemic injury in rats; protective effects and antioxidant activity.

J Renal Inj Prev 2017 24;6(2):93-98. Epub 2016 Nov 24.

Department of Pharmacotherapy, Faculty of pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.

Renal dysfunction is caused by ischemia-reperfusion (I/R) injury, which is a common problem in kidney surgery or kidney transplantation. The human body consists of enormous complex antioxidant systems, which inquires adequate selenium (Se) absorption for normal physiologic function. It is known that Se has some antioxidant effects. In the present research, effects of the Se on damages caused by I/R injury investigated. In this experimental research, four groups of rats (weighing 220±10 g) used, include control group, I/R group, healthy group treated with Se for two weeks, and I/R group with two-week Se treatment. On the test day, I/R was treated in both right and left renal arteries for 45 minutes and the reperfusion was done for 24 hours. In I/R group, the amount of urea and serum creatinine (Cr) was an injury indicator of the kidney cells which showed a significant increase compared with the control group. When the treatment with Se significantly reduced these indicators, glutathione (GSH) enzyme levels reduced significantly in the second group and the enzyme levels increased due to Se treatment in the fourth group. Furthermore, malondialdehyde (MDA) enzyme levels increased in I/R group due to the Se treatment in the fourth group which was significantly reduced. In addition, the tissue damage was reduced in the fourth group compared with I/R group. Se has a protective effect against the I/R injury. This effect might be due to the antioxidant properties of Se.
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http://dx.doi.org/10.15171/jrip.2017.18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5423291PMC
November 2016

Evaluation of alginate hydrogel cytotoxicity on three-dimensional culture of type A spermatogonial stem cells.

Int J Biol Macromol 2017 Feb 27;95:888-894. Epub 2016 Oct 27.

Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran. Electronic address:

The culture of spermatogonial cells for future transplantation, based on the specific biology of these cells is important and necessary. Recently, the use of scaffolds especially alginate for culturing stem cells has been the focus of many researchers. The aim of this study was to evaluate the cytotoxicity of alginate hydrogels to cultures of type A spermatogonial stem cells. Spermatogonial stem cells of 6day-old immature mice were isolated by surgery; thereafter, the cells were purified by MACS using antibodies against thy-1 and C-kit and cultured on a layer of laminin. After purification, spermatogonial stem cells were encapsulated in alginate hydrogels. After one month of encapsulation and culture in DMEM culture medium containing 10ng/ml GDNF, cells were removed from hydrogel and were examined for viability, cell morphology and structure, cytotoxicity and expression of apoptosis genes Fas, P53, Bax, Bcl2, Caspase3 by staining with trypan blue, scanning electron microscopy, LDH test, and Real time PCR, respectively. The encapsulation did not change the morphology and viability of spermatogonial stem cells. Investigations showed that spermatogonial stem cells preserve by the high viability (74.08%) and cytotoxicity of alginate hydrogel was estimated to be 5%. Expression of Fas gene increased in main group compared with the control group, and expression of Bax and P53 was reduced in main group compared with the control group. Expression of Bcl2 and Caspase3 genes did not show any significant difference between the main group and the control group. Considering the lack of cytotoxicity and antioxidant properties of alginate hydrogel scaffold and high viability of cells, this three-dimensional scaffold is applicable for culturing and encapsulation of spermatogonial stem cells.
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http://dx.doi.org/10.1016/j.ijbiomac.2016.10.074DOI Listing
February 2017

Expression of liver alpha-amylase in obese mouse hepatocytes.

Gastroenterol Hepatol Bed Bench 2016 ;9(4):278-285

Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Deptartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.

Aim: The aim of this study is to demonstrate the relation between the expression of liver alpha-amylase and obesity.

Background: Alpha-amylase catalyses the hydrolysis of 1, 4-alpha-glucosidic linkages in polysaccharides and has three main subtypes, including: salivary, pancreatic, and hepatic. Hepatic alpha-amylase is involved in glycogen metabolism, and has a role in obesity and its management. In this study, we aimed to analyze the expression of liver alpha-amylase in overweight and obese mouse.

Material And Methods: In this study, NMRI male mice were randomly divided into two groups. The sample group (obese) took a high-fat and carbohydrate diet, while the control group (normal) took a laboratory pellet chow for eight weeks. During this period, their weight was measured. After eight weeks, liver hepatocytes were isolated using an enzymatic digestion method. Immunocytochemistry (ICC) and flow cytometry analysis were performed to measure alpha amylase protein expression in mouse liver hepatocyte cells.

Results: A significant difference in the body weight was observed between the two groups (p<0.05). The qualitative protein expression of liver alpha-amylase was found to be higher in the obese group in both tests (immunocytochemistry and flow cytometry). Animals from the test group presented higher alpha-amylase expression, which suggests that this hepatic protein may constitute a potential indicator of susceptibility for fat tissue accumulation and obesity. The present data demonstrates an increased expression of liver amylase in obese mice.

Conclusion: These results suggest that liver amylase secretion might be useful for predicting susceptibility to obesity induced by consumption of a high-fat and carbohydrate diet.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5118852PMC
January 2016

Selenium Ameliorate Peripheral Nerve Ischemic-Reperfusion Injury via Decreased TNF-α.

Biol Trace Elem Res 2017 Apr 6;176(2):328-337. Epub 2016 Sep 6.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Selenium is considered as a trace element that plays antioxidant role in the body. So, the aim of this study was to evaluate the effect of selenium on ameliorating of sciatic nerve ischemia-reperfusion injury. Eighty (80) adult male Wistar rats weighing 250-300 g were used. They were divided into 10 groups (n = 8). Then, femoral vessels were obstructed by using 4/0 silk and splitknot techniques. After 3-h ischemia for all the groups, reperfusion was applied for different periods: 3, 7, 14, and 28 days. In half of each experimental group, 0.2 mg/kg selenium was injected intraperitoneally, coinciding with ischemia. After reperfusion, according to the grouping, rats were killed by using high dose of anesthetic drug and then sciatic nerve was removed and fixed. Then, tissue samples were processed and subsequently stained with hematoxylin-eosin, apoptosis, and immunohistochemistry stains. On the third day of reperfusion, the amount of TNF-α as an inflammatory marker of ischemia-reperfusion acute phase increased. On the seventh day of reperfusion, the amount of NF-кB as an apoptotic index and infiltration of mast cells increased in the tissue as a result of development of inflammation. But, on the 14th day of reperfusion, the amount of NF-кB as an apoptotic index decreased to the lowest amount. On the 28th day of reperfusion, the amount of TNF-α as an inflammatory marker decreased to its lowest level. Prescription of selenium concurrent with development of ischemia can reduce the damage caused by sciatic nerve ischemia-reperfusion.
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http://dx.doi.org/10.1007/s12011-016-0836-7DOI Listing
April 2017

Supplementation freeze-thawed media with selenium protect adipose-derived mesenchymal stem cells from freeze-thawed induced injury.

Cryobiology 2016 10 19;73(2):135-9. Epub 2016 Aug 19.

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Anatomical Sciences, Lorestan University of Medical Science, Khorramabad, Iran. Electronic address:

Successful freezed-thaw of adipose-derived mesenchymal stem cells (ADMSCs) could be a major step in regenerative medicine as well as in the cloning of animal breeds. The aim of this study was to evaluate the efficacy of selenium on the optimizing of freezed-thaw media in the ADMSCs. ADMSCs were extracted from NMRI mice and purified with positive selection Monoclonal CD105 Antibody (PE) and negative selection Monoclonal CD31 and CD45 Antibody using MACS method as well as differentiation to adipose and bone tissue. ADMSCs were divided into four groups. ADMSCs were freezed-thaw under standard condition with or without the addition of 5 ng/ml selenium to both the cryopreservation and thawing solutions. Frozen cells were thawed after four months and viability and cytotoxicity of the cells were analyzed by the Trypan blue test and MTT assay respectively. RNA was extracted and cDNA was synthesized and the expression of apoptotic genes (P53, Fas, Bax, Caspase3, and Bcl2) was examined using Real time-PCR Rotor gene 2009. This study compares slow and rapid methods of cryopreservation. After thawing, viability of the cells treated with selenium was higher than the control group in rapid and slow cryopreserved ADMSCs. Also, the percentage of living cells in the slow cooling method was considerably more than with the rapid cooling method. After analysis of the results using Real time-PCR, the Bcl2 gene was shown to be expressed in both the rapid and slow cooling methods. In the rapid cooling group in addition to the BCL-2 gene, p53 was also expressed. It appears that selenium prevented the apoptotic genes from expression due to its anti-apoptotic effects. The slow cooling method is better and more optimized for ADMSCs protecting them from oxidative damage to a greater extent compared to the rapid cooling method.
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http://dx.doi.org/10.1016/j.cryobiol.2016.08.009DOI Listing
October 2016

Prevalence of HCV Infections and Co-Infection With HBV and HIV and Associated Risk Factors Among Addicts in Drug Treatment Centers, Lorestan Province, Iran.

Int J High Risk Behav Addict 2016 Mar 18;5(1):e25028. Epub 2016 Jan 18.

Deputy of Health Services, Lorestan University of Medical Sciences, Khorramabad, IR Iran.

Background: Hepatitis C is an infectious disease caused by blood-borne pathogen, hepatitis C virus (HCV).

Objectives: The purpose of this study was to investigate the prevalence of HCV infection and associated risk factors among addicts in drug treatment centers in Lorestan Province, Iran.

Patients And Methods: A cross-sectional sero-behavioral survey was given to drug addicts in the drug treatment centers of Khorramabad, Lorestan Province, Iran during June 2012 - March 2013. Drug addicts were interviewed using a standard questionnaire including demographic, imprisonment history, and HCV-related risk behavior items. Thereafter, the sera drawn from the participants were tested for anti-HCV antibody (Ab), anti-human immunodeficiency virus (HIV) Ab, and hepatitis B surface antigen (HBsAg).

Results: The mean age of the cohorts was 31.7. Up to 60.2% of drug users had educational levels less than high school, 67.5% were self-employed, and 32.5% were office workers. The mean duration of drug injection was 6.8 years. Statistical analyses indicated that the prevalence of HCV among drug addicts was positively associated with age, past incarceration, drug injection history, the duration of drug use, and tattooing. In addition, 16.23% of volunteers were HCV-positive. Of those infected with HCV, 1.10% was co-infected with HBV, 2.95% were positive for HIV, and 0.36% of HCV-positive cases were infected with all three viruses.

Conclusions: The high prevalence of HCV infection among this group implies a high rate of transmission and exposure to the risk of serious diseases. It is important that the high prevalence of HCV infection be taken into consideration to control further transmission of this infection.
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http://dx.doi.org/10.5812/ijhrba.25028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859996PMC
March 2016

The effect of progesterone treatment after ovarian induction on endometrial VEGF gene expression and its receptors in mice at pre-implantation time.

Iran J Basic Med Sci 2016 Mar;19(3):252-7

Deptartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.

Objectives: Progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. It is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time.

Material And Methods: Adult female NMRI mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimulation]. Uterine horn samples removed at pre-implantation time in each group. Motic image Plus 2 software was used to assess the quantitative vascular parameters of endometrium. Gene expression was determined for vascular endothelial growth factor (VEGF), FMS-like tyrosine kinase (FLT) and Kinase insert domain protein receptor (FLK) genes using the real time PCR method. Data analysis was done with LinReg PCR and Rest-RG software.

Results: Comparison between progestrone treated mice after ovarian stimulation with control group showed that increase in rate of VEGF gene expression [0.775] and decrease in rate of FLK [6.072] and FLT [1.711] gene expression. Analysis of the data on quantitative vascular parameters were indicated remarkable increase in quantitative vascular parameters of progestrone treated mice compare to control group.

Conclusion: Biological effect of progestrone on the vascular changes after ovarian stimulation resulted in an increase in VEGF receptors experession, it seems that induced angiogenesis by progesterone could result in better condition for implantation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4834114PMC
March 2016

Quercetin ameliorates peripheral nerve ischemia-reperfusion injury through the NF-kappa B pathway.

Anat Sci Int 2017 Jun 14;92(3):330-337. Epub 2016 Mar 14.

Stem cells and tissue engineering research center, Shahroud University of Medical Sciences, Shahroud, Iran.

This study aimed to investigate the protective effect of quercetin against ischemia-reperfusion (IR) injury induced in the sciatic nerve of the rat. Quercetin (20 mg/kg) was given during ischemia just before reperfusion. Four groups of rats (Q+IR3, Q+IR7, Q+IR14, and Q+IR28) received 3, 7, 14, and 28 days of reperfusion, respectively, after the intraperitoneal injection of quercetin. After reperfusion, a behavioral test was performed and the sciatic functional index was calculated. Each sciatic nerve was stained to check for edema and ischemic fiber degeneration. Immunohistochemical staining was performed to detect TNF-alpha and NF-kappa B, and TUNEL staining was carried out to detect apoptosis. The Q+IR3, Q+IR7, and Q+IR14 groups showed significantly increased behavioral scores and ameliorated sciatic functional index values compared to IR-injured rats that received vehicle alone during ischemia and then the same period of reperfusion. The Q+IR3, Q+IR7, Q+IR14, and Q+IR28 groups presented significant ischemic fiber degeneration (IFD), TNF-alpha expression, and apoptosis as compared with the IR-injured and perfused rats that did not receive quercetin. The Q+IR3, Q+IR7, and Q+IR28 groups also exhibited significantly decreased NF-kappa B expression (p < 0.001, p = 0.001, p = 0.026) as compared with the IR-injured rats that were perfused but did not receive quercetin. These results imply that quercetin may be beneficial in the treatment of sciatic IR injury because of its antiapoptotic and antiinflammatory effects and its ability to decrease the expression of NF-kappa B.
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http://dx.doi.org/10.1007/s12565-016-0336-zDOI Listing
June 2017

Synergistic effects of nitric oxide and exercise on revascularisation in the infarcted ventricle in a murine model of myocardial infarction.

EXCLI J 2015 14;14:1104-15. Epub 2015 Oct 14.

Department of Physiology, Physiology research center, School of Medicine, Iran Universty of Medical Sciences, Tehran, Iran.

It has been shown that density of microvessels decreases in the left ventricular after myocardial infarction (MI). The change of angiogenic and angiostatic factors as the main factors in revascularisation after exercise training in area at risk is not determined yet in MI. Therefore, the aim of the present study was the effect of exercise training and L-arginine supplementation on area at risk angiogenesis in myocardial infarction rat. Four weeks after surgery (Left Anterior Descending Coronary artery Ligation), myocardial infarction rats were divided into 4 groups: Sedentary rats (Sed-MI); L-arginine supplementation (La-MI); Exercise training (Ex-MI) and Exercise + L-arginine (Ex+La). Exercise training (ET) lasted for 10 weeks at 17 m/min for 10-50 min day(-1). Rats in the L-arginine-treated groups drank water containing 4 % L-arginine. After ET and L-arginine supplementation, ventricular function was evaluated and angiogenic and angiostatic indices were measured at ~1 mm from the edge of scar tissue (area at risk). Statistical analysis revealed that gene expression of VEGF as an angiogenic factor, angiostatin as an angiostatic factor and caspase-3 at area at risk decrease significantly in response to exercise training compared to the sedentary group. The capillary and arteriolar density in the Ex groups were significantly higher than those of the Sed groups. Compared to the Ex-MI group, the Ex+La group showed a markedly increase in capillary to fiber ratio. No significant differences were found in infarct size among the four groups, but cardiac function increased in response to exercise. Exercise training increases revascularization at area at risk by reduction of angiostatin. L-arginine supplementation causes additional effects on exercise-induced angiogenesis by preventing more reduction of VEGF gene expression in response to exercise. These improvements, in turn, increase left ventricular systolic function and decrease mortality in myocardial infarction rats.
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http://dx.doi.org/10.17179/excli2015-510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746998PMC
February 2016

Evaluation of the Persian Transcript of the COPD Assessment Test in the Measurement of COPD Health Status in Iranian COPD Patients.

Glob J Health Sci 2015 Oct 20;8(5):225-30. Epub 2015 Oct 20.

School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

Aim: Chronic obstructive pulmonary disease is a completely irreversible obstructive airway disease. The COPD assessment test (CAT) is one of the standard methods for the clinical assessment of the disease, which is translated into Persian. This study investigated the reliability of the test and its relationship with the severity of the disease.

Methods: In this cross-sectional study, 120 patients filled out the Persian transcript of the test. After two weeks, the patients filled out the CAT test again. Obstruction severity was determined for all the patients using spirometry, and the patients were categorized into four groups according to the Global Initiative for Chronic Obstructive Lung Disease criteria. The relationship between the test scores and the disease severity wan validated.

Results: The mean age of the patients was 51.5 years. The Cronbach's alpha coefficient of the Persian transcript of the test was 0.872 in the first time, and 0.885 in the second time. Intragroup reliability, test re-test and intragroup correlations were significant for all the questions (<0.001). The relationship between the test mean score and obstruction severity was significant, and the correlation between disease categorization in accordance with obstruction severity and categorization according to the test score was significant as well.

Conclusion: The Persian transcript of the assessment test for COPD was reliable and is directly related to the disease severity according to airflow limitation.
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http://dx.doi.org/10.5539/gjhs.v8n5p225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877241PMC
October 2015

Evaluation of Trends in the Use of Complementary and Alternative Medicine in Health Centers in Khorramabad (West of Iran).

Glob J Health Sci 2015 Jun 4;8(2):72-6. Epub 2015 Jun 4.

School of medicine, Lorestan University of Medical Sciences, Khorramabad, Iran..

Aim: To determine the use of most popular forms of complementary and alternative medicine(CAM), sociodemographic characteristics of CAM users, and communication between CAM users and their physicians by adult Iranian in Khorramabad city.

Methods: This cross-sectional study was carried out on clients who were at least 15 years in age referring to health centers and hospitals in Khorramabad town in 2014. A multi-part questionnaire was used to gather information. The demographic data and details regarding usage (number of times and underlying reasons) of different kinds of complementary and traditional medicine in the past were gathered using a questionnaire.

Results: In this study 790 subjects were surveyed using the questionnaire. The mean age of the participant was 38.9 years. 79.8% of the subjects had used at least one of the methods of complementary medicine. Among the participants, 58.2% had used at least one of the complementary medicines in the previous year. Herbal medicine and prayers treatment had the highest use with 69.2% and 37.2%, respectively. Concerns of the side effects of medical therapy, beliefs in less risky being and fewer side effects of complementary medicine, Dissatisfaction of General Practitioners, the increase of being-well feelings in physical conditions, and were among the most important reasons of inclination towards such treatment methods.

Conclusion: The analysis of using complementary medicine among people is the first step for planning proper use of the beneficial methods of complementary medicine and the prevention of inefficient and harmful methods in this respect.
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http://dx.doi.org/10.5539/gjhs.v8n2p72DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803943PMC
June 2015

Effects of Vitamin D Intake on FEV1 and COPD Exacerbation: A Randomized Clinical Trial Study.

Glob J Health Sci 2015 Jan 14;7(4):243-8. Epub 2015 Jan 14.

School of medicine, Lorestan University of Medical Sciences, Khorramabad, Iran..

Aim: This study aimed to evaluate the effects of vitamin D intake on COPD exacerbation and FEV1 in the patients with severe and very severe COPD.

Methods: This double blind placebo control randomized clinical trial study was done in the Ashayer university hospital in Khorramabad in 2012. Eighty eight patients with severe and very severe COPD were randomly selected from those who recoursed to the internal medicine clinic of Ashayer hospital. They were randomly allocated to case and placebo group. The patients received routine treatment for COPD. Along with the routine treatment, placebo group received 100,000 IU of oral vitamin D per month, for 6 months. Data was analyzed using SPSS computer software, paired t-test, independent t-test, non parametric t-test and Pearson correlation coefficients.

Results: In each group, there were 44 patients. After the intervention, there were significant differences in FEV1 and the number of COPD exacerbation between the case and control group patients. Also, after the study, in the case group, FEV1 was increased and the number of COPD exacerbation was decreased significantly.

Conclusion: Vitamin D intake decreased COPD exacerbation and improved FEV1 in the patients with severe and very severe COPD. It is suggested that baseline serum vitamin D levels will recorded in similar studies and the effect of vitamin D intake will evaluated regarding the baseline serum vitamin D levels.
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http://dx.doi.org/10.5539/gjhs.v7n4p243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4802087PMC
January 2015

Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.

Biol Trace Elem Res 2015 Apr 28;164(2):205-11. Epub 2014 Dec 28.

Razi Herbal Medicines Research Center and Department of Anatomy, Lorestan University of Medical Sciences, Khorramabad, Lorestan, Islamic Republic of Iran,

Selenium is a trace element that has antioxidant and neuroprotective effects. The aim of this study is to investigate the effects of selenium in reducing ischemia-reperfusion injury of the gastrocnemius muscle. In this experimental study, 80 adult male Wistar rats weighing 250-300 g were divided into ten groups (N = 8 per group). Group 1 is control group (without ischemia-reperfusion). Group 2 received 0.2 mg/kg selenium. Group 3 received ischemia + 3 d reperfusion + 0.2 mg/kg selenium, group 4 received ischemia + 3 d reperfusion + 0.2 mg/kg placebo, group 5 received ischemia + 7 d reperfusion + 0.2 mg/kg selenium, group 6 received ischemia + 7 d reperfusion + 0.2 mg/kg placebo, group 7 received ischemia + 14 d reperfusion + 0.2 mg/kg selenium, group 8 received ischemia + 14 d reperfusion + 0.2 mg/kg placebo, group 9 received ischemia + 28 d reperfusion + 0.2 mg/kg selenium and group 10 received ischemia + 3 d reperfusion + 0.2 mg/kg placebo. External iliac artery blocked for 3 h. After reperfusion, rats killed and gastrocnemius muscle removed, fixed, and tissue processing performed. Samples stained with hematoxylin-eosin for edema evaluation, toluidine blue for mast cell infiltration evaluation and immunohistochemistry for detection TNF-alpha and NF-kappa B proteins. Comparison of mast cell infiltration, edema of the interstitial fluid on the tissue, expression of TNF-alpha protein, and expression of NF-kappa B protein in the groups that received selenium with corresponding placebo group showed that selenium can reduce edema, mast cell infiltration, and TNF-alpha expression and inactivated NF-kappa B. The use of selenium simultaneously with creating ischemia can reduce ischemia-reperfusion injury of the gastrocnemius muscle.
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http://dx.doi.org/10.1007/s12011-014-0218-yDOI Listing
April 2015

Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice.

Iran J Basic Med Sci 2014 Feb;17(2):93-9

Department of Medical Genetics, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Objective(s): Transplantation quality improvement and reduction of cellular damage are important goals that are now considered by researchers. Melatonin is secreted from the pineal gland and some organs such as testes. According to beneficial effects of melatonin (such as its antioxidant and antiapoptotic properties), researchers have proposed that the use of melatonin may improve transplantation quality. The aim of this study was to investigate the effects of melatonin on the spermatogonial stem cells transplantation in the azoospermic mice.

Materials And Methods: The testes of the BALB/c mice pups (6-day-old) after vitrified-thawed, were digested with enzymes (collagenase, DNaseΙ, trypsin-EDTA) to disperse the cells. The SSCs, type A, were isolated from the rest of testicular cells by MACS. Spermatogonial stem cells were labeled with PKH26 fluorescent kit. Labeled spermatogonial stem cells were transplanted into the testes of infertile mice (busulfan 40 mg/kg). The mice died two months after transplantation and the efficiency of spermatogenesis was investigated. TNP2 and hematoxyline-eosin staining were used to detect the efficiency of cell transplantation.

Results: TNP2 were detected in the samples that received melatonin and spermatogonial stem cells transplantation, simultaneously. TNP2 was not detectable in the transplant recipient mice that received placebo for 10 weeks (control group). According to hematoxyline-eosin staining, melatonin improved structure of testes.

Conclusion: Administration of melatonin (20 mg/kg) simultaneously with transplantation of spermatogonial stem cells in azoospermia mouse testis increases the efficiency of transplantation and improves structural properties of the testes tissue.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3976745PMC
February 2014