Publications by authors named "Mohammad Soleimani"

146 Publications

Molecular detection of in biopsy samples of intervertebral disc with modic changes in patients undergoing herniated disc surgery.

Iran J Microbiol 2020 Dec;12(6):516-521

Department of Laboratory Sciences, Faculty of Paramedical Sciences, Aja University of Medical Sciences, Tehran, Iran.

Background And Objectives: Recent studies have hypothesized that sterile disc infection with the anaerobic , recently renamed , occurs in people with intervertebral disc (IVD) herniation. This study aimed to examine the presence of in patients who have Low back pain (LBP) with Modic changes observed in their Magnetic Resonance Imaging (MRI).

Materials And Methods: Thirty-seven patients who were candidates for surgery due to disc herniation and demonstrated Modic changes in MRI were included in the study. Before the surgery, the level of pain in patients was assessed using the visual analog score (VAS). All patients were asked to fill in the Oswestry Low Back Pain Disability Questionnaire. Intervertebral disc changes observed in MRI were recorded for all patients. Then, during surgery, sterile intervertebral disc samples were taken. detection was performed using PCR in the laboratory. Data analysis with Chi-squared test, independent samples t-test, and Mann-Whitney U test in SPSS 18.0.

Results: The mean age of 37 patients equaled 43.64 years and the mean duration of symptoms was 11.05 months. In molecular examination, of the 37 individuals, the genome of was positive in 23 cases (62.2%) and negative in 14 (37.8%). The relationship between VAS, disability score, changes in MRI, and patients' age with the positivity of the intervertebral disc sample was also assessed. Of these variables, only age was significantly correlated with the positive molecular finding, such that with an increase in age, the probability of positive findings was increased (p = 0.022).

Conclusion: Based on the results, lumbar disc infection with may play a significant role in causing Modic changes and the progression of the disease in patients with LBP.
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http://dx.doi.org/10.18502/ijm.v12i6.5025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884273PMC
December 2020

Correction to: Psychometric Properties of the Persian Version of Spiritual Well-Being Scale in Patients with Acute Myocardial Infarction.

J Relig Health 2021 Feb 11. Epub 2021 Feb 11.

Department of Biostatistics and Medical Informatics, Faculty of Medicine, Ahi Evran University, Kırşehir, Turkey.

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http://dx.doi.org/10.1007/s10943-021-01204-xDOI Listing
February 2021

Infectious keratitis: trends in microbiological and antibiotic sensitivity patterns.

Eye (Lond) 2021 Jan 19. Epub 2021 Jan 19.

Ophthalmology Department and Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Objectives: To report the spectrum and trends of isolated microorganisms and their antibiotic susceptibility profile in patients with infectious keratitis in a 6-year period at a referral centre in Tehran.

Methods: The microbiology records of all corneal scrapings with a diagnosis of infectious keratitis were reviewed.

Results: A total of 6282 corneal scrapings were performed during the study period, of which 2479 (39.5%) samples were culture positive. Pseudomonas aeruginosa was found to be the most common causative agent in patients with keratitis, although Streptococcus pneumonia was the most prevalently isolated microorganism in patients older than 50 years. Fusarium sp. was the most common responsible pathogen in patients with fungal keratitis. The prevalence of bacterial keratitis due to gram positive microorganisms increased over time, however the number of Pseudomonas keratitis decreased in the second half of the study. Gram negative organisms showed a good sensitivity to levofloxacin, however, 34.1% of S. aureus isolates and 29.7% of coagulase negative staphylococci were resistant to this antibiotic. The odds of ciprofloxacin and levofloxacin resistance increased 1.25 and 1.15 for each 1-year increase in culture date, respectively (P < 0.001, P = 0.004).

Conclusions: We documented an increasing trend in the percentage of gram positive bacteria. Levofloxacin monotherapy might still be a good option in patients with gram negative bacterial keratitis, however owing to increasing resistance of staphylococci to fluoroquinolones, a regimen consisting of a combination of fortified antibiotics may be more effective in staphylococcal keratitis.
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http://dx.doi.org/10.1038/s41433-020-01378-wDOI Listing
January 2021

Management of refractory Acanthamoeba keratitis, two cases.

Parasitol Res 2021 Mar 6;120(3):1121-1124. Epub 2021 Jan 6.

Ocular Trauma and Emergency Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Acanthamoeba keratitis is a serious infection of the eye that can result in permanent visual impairment or blindness, caused by free-living amoebae of the genus Acanthamoeba. Early diagnosis is necessary for effective treatment of Acanthamoeba keratitis. Acanthamoeba is abundant in nature and can be found in water, soil, and air. Acanthamoeba keratitis is usually diagnosed by culture from a scraping of the eye or by confocal microscopy. In this paper, two complicated Acanthamoeba keratitis cases are reported.
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http://dx.doi.org/10.1007/s00436-020-06997-6DOI Listing
March 2021

Predictive factors associated with death depression in women with breast cancer.

Death Stud 2020 Dec 30:1-11. Epub 2020 Dec 30.

Social Determinants of Health Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.

A cross-sectional study with a convenience sample of 241 patients with breast cancer was conducted to investigate death depression and its explanatory factors in Iranian women who were diagnosed and undergoing treatment for breast cancer. Patients with significantly higher death depression were those who were housewives ( = 0.6,  = 0.016), unskilled workers ( = 15.8,  = 0.002), and retired ( = 13.1,  = 0.002) as compared with those who were professionally employed with higher socio-economic status ( = 17.4,  = 0.002); and those receiving combination therapy as compared to surgery only ( = 6.3,  = 0.02). For patients with higher spiritual health scores, death depression was significantly lower ( = -0.2,  < 0.001).
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http://dx.doi.org/10.1080/07481187.2020.1864682DOI Listing
December 2020

Drug-induced ectropion following the chronic use of topical Natamycin.

J Ophthalmic Inflamm Infect 2020 Dec 21;10(1):38. Epub 2020 Dec 21.

Ocular Trauma and Emergency Department, Farabi Eye Hospital, Tehran, Iran.

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http://dx.doi.org/10.1186/s12348-020-00230-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750383PMC
December 2020

Antibacterial Activity of Root Methanol Extract against Most Common Burn Wound Pathogens.

Iran J Med Sci 2020 Nov;45(6):444-450

Department of Genetics and Advanced Medical Technology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Background: Burn wound infection and sepsis are serious medical conditions requiring prompt intervention. Plants are a good natural source for the development of novel, safe, and cost-effective antibacterial agents. The objective of the present study was to assess the antibacterial potential of aqueous, chloroform, and methanol extracts of the () root against the most common burn wound pathogens.

Methods: The present experimental study was conducted at Shiraz University of Medical Sciences (Shiraz, Iran) during 2018-2019. The antibacterial activity of the total plant extract was assayed using the broth microdilution method. Fractionation was performed using a separation funnel and solvents with different polarities. Broth microdilution and agar well diffusion assays were performed to determine the antibacterial potential of the obtained fractions. Quantitative and qualitative phytochemical analyses were performed to confirm the presence of secondary metabolites in both the total extract and the fractions.

Results: Methanolic extract of root exhibited antibacterial activity against all tested bacterial strains, especially against Methicillin-resistant (MRSA) isolates. This extract, compared to the aqueous and chloroformic extracts, exhibited the presence of active antibacterial compounds. The quantitative and qualitative results of phytochemical screening showed that phenols and flavonoids were the main antibacterial compounds in the methanolic extract of the plant.

Conclusion: For the first time, we demonstrated the antibacterial activity of the root against MRSA isolates and other common burn wound pathogens.
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http://dx.doi.org/10.30476/ijms.2019.82071DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707634PMC
November 2020

Current diagnostic tools and management modalities of Nocardia keratitis.

J Ophthalmic Inflamm Infect 2020 Dec 2;10(1):36. Epub 2020 Dec 2.

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Nocardia species are an uncommon but important cause of keratitis. The purpose of this review is to discus previous published papers relation to the epidemiology, etiology, diagnosis and management of Nocardia keratitis. Nocardia asteroides is the most frequently reported from Nocardia keratitis. Pain, photophobia, blepharospasm and lid swelling are mainly clinical manifestations. Usual risk factors for Nocardia keratitis are trauma, surgery, corticosteroids, and contact lens wear. Several antibiotics were used for treatment of Nocardia infection but according to studies, topical amikacin is the drug of choice for Nocardia keratitis. Topical steroid should not prescribe in these patients. In conclusion, although Nocardia keratitis is rare, early diagnosis and treatment are essential to prevent any scar formation and preserve a good visual acuity.
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http://dx.doi.org/10.1186/s12348-020-00228-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7710777PMC
December 2020

A ten-year report of microbial keratitis in pediatric population under five years in a tertiary eye center.

J Ophthalmic Inflamm Infect 2020 Nov 27;10(1):35. Epub 2020 Nov 27.

Ocular Trauma and Emergency Department, Eye Research Center, Farabi eye hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To report characteristics of microbial keratitis in pediatric patients under five years.

Methods: Patients with infectious keratitis under the age of 5 years were included in this retrospective cross-sectional study for ten years. All patients were admitted and corneal scraping was performed in 81 children. Fortified empiric antibiotic eye drops including cefazolin (50 mg/cc) and amikacin (20 mg/cc) were started and the antibiotic regimen was continued or changed according to culture results. In the case of fungal keratitis, topical voriconazole (10 mg/cc) or natamycin (50 mg/cc) and topical chloramphenicol (5 mg/cc) were started. A tectonic procedure was done when corneal thinning or perforation was present.

Results: Ninety-Three Patients between 1 to 60 months with a mean age of 33 ± 18 months old with corneal ulcer were included in the study. The most common risk factor was trauma (40.9%) followed by contact lens use (8.6%). Cultures were negative for microbial growth in 28 (30.1%) patients. The most common pathogens were S. epidermidis (10.8%) and P. aeruginosa (10.8%). Fluoroquinolone antibiotics (ciprofloxacin; 93.8% sensitivity) were the most potent antibiotic against bacterial pathogens. Forty-one patients underwent tectonic procedures, which the most common ones were cyanoacrylate glue 18.3% followed by keratoplasty 16.1%.

Conclusion: This study emphasizes the role of trauma as the primary cause and S. epidermidis as the most frequent microorganism in pediatric keratitis; according to antibiogram results and poor cooperation of patients under five years, monotherapy with fluoroquinolones could be a good regimen in small non-central lesions without thinning.
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http://dx.doi.org/10.1186/s12348-020-00227-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695777PMC
November 2020

Rationing access to total hip and total knee replacement in the Islamic Republic of Iran to reduce unnecessary costs: policy brief.

East Mediterr Health J 2020 Nov 11;26(11):1396-1402. Epub 2020 Nov 11.

Faculty of Pharmaceutical Science, Isfahan University of Medical Sciences, Isfahan, Islamic Republic of Iran.

Rationing health services is an inseparable part of the health system of any country in order to achieve universal health coverage. Elective surgery for total hip and total knee replacement places a high financial burden on health systems. Such surgery should be done in a way to ensure that the people who most need it receive the service. Models for rationing total hip and knee replacement surgery were reviewed to suggest the best policy for rationing such surgery in the Islamic Republic of Iran. We propose a system with three main tools: clinical guidelines, gate keepers and waiting lists, with shared decision-making as an auxiliary tool. Patients should be scored at the primary health care level based on clinical and radiographic examination, alternative treatments (conservative treatments) and risk factors, with a set threshold for referral. Patients whose scores are above the threshold should be referred to secondary health care. These patients should be assessed again by specialists based on age, bone condition, surgery risk and other alternative treatments. Patients whose scores are above the threshold should be put on the waiting list for surgery.
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http://dx.doi.org/10.26719/emhj.20.109DOI Listing
November 2020

Modifying the thickness, pore size, and composition of diatom frustule in Pinnularia sp. with Al ions.

Sci Rep 2020 11 11;10(1):19498. Epub 2020 Nov 11.

Laboratory of Physical Chemistry, and Center for Multiscale Electron Microscopy, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, Groene Loper 5, 5612 AE, Eindhoven, The Netherlands.

Diatoms are unicellular photosynthetic algae that produce a silica exoskeleton (frustule) which exposes a highly ordered nano to micro scale morphology. In recent years there has been a growing interest in modifying diatom frustules for technological applications. This is achieved by adding non-essential metals to the growth medium of diatoms which in turn modifies morphology, composition, and resulting properties of the frustule. Here, we investigate the frustule formation in diatom Pinnularia sp., including changes to overall morphology, silica thickness, and composition, in the presence of Al ions at different concentrations. Our results show that in the presence of Al the total silica uptake from the growth medium increases, although a decrease in the growth rate is observed. This leads to a higher inorganic content per diatom resulting in a decreased pore diameter and a thicker frustule as evidenced by electron microscopy. Furthermore, Al solid-state NMR, FIB-SEM, and EDS results confirm that Al becomes incorporated into the frustule during the silicification process, thus, improving hydrolysis resistance. This approach may be extended to a broad range of elements and diatom species towards the scalable production of silica materials with tunable hierarchical morphology and chemical composition.
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http://dx.doi.org/10.1038/s41598-020-76318-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7658998PMC
November 2020

Management Strategies of Ocular Chemical Burns: Current Perspectives.

Clin Ophthalmol 2020 15;14:2687-2699. Epub 2020 Sep 15.

Ocular Trauma and Emergency Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Ocular chemical burns are absolute ophthalmic emergencies and require immediate management to minimize devastating sequelae. Management of alkali and acid burns is started at the scene of the accident by copious irrigation. Treatment is directed at improving epithelial integrity and stromal stability, reduction of undue inflammation, and prevention or timely management of complications. To ascertain the best possible outcome, numerous biological medications and surgical interventions have been merged into conventional therapeutic regimens. These include autologous and umbilical cord serum preparations, platelet-rich plasma, amniotic membrane transplantation, limbal stem-cell transplantation, and anti-angiogenic agents.
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http://dx.doi.org/10.2147/OPTH.S235873DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7501954PMC
September 2020

Isolation and identification of free-living amoeba from the hot springs and beaches of the Caspian Sea.

Parasite Epidemiol Control 2020 Aug 3;10:e00151. Epub 2020 May 3.

Ocular Trauma and Emergency Department, Farabi eye hospital, Tehran university of Medical Sciences, Tehran, Iran.

Free-living amoeba (FLA) such as , , and have been identified from both natural and human-made environments such as Hot springs and spa. causes Primary Amoebic Meningoencephalitis (PAM), while and cause chronic granulomatous encephalitis. also can cause cutaneous lesions and Amoebic Keratitis (AK) that is associated with contact lens use or corneal trauma. FLA are known to serve as host of and vehicles for diverse intracellular organisms. This study aimed was to identify the presence of FLA in the hot springs and beaches of the Caspian Sea in Ramsar tourist town located in the northern part of Iran. Water samples were collected in sterile bottles and were transferred to the laboratory. One litre of each sample passed through the nitrocellulose membrane filter. Each filter insert was then placed in non-nutrient agar plates already seeded with lawn culture of . Positive samples were analyzed by morphological keys and Polymerase chain reaction (PCR) using 18S rDNA gene and ITS region to identify amoeba isolates. A total of 81 water sampled were tasted. After identified using the morphological key and PCR assay, 54 (66.6%) of the samples were positive for FLA. Ten of the samples were identified as (belong to T3, T4, and T5 genotypes), three as , four as (3  and 1 ). Only one sample was positive . The presence of thermotolerant FLA in the Hot springs and beaches of the Caspian Sea as places for recreational purposes or wellness may be a potential health risk.
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http://dx.doi.org/10.1016/j.parepi.2020.e00151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474157PMC
August 2020

The effect of psychoeducational intervention, based on a self-regulation model on menstrual distress in adolescents: a protocol of a randomized controlled trial.

Trials 2020 Aug 27;21(1):747. Epub 2020 Aug 27.

Social Determinants of Health Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Shahid Bahonar Blvd, Qazvin, 3419759811, Iran.

Introduction: Menstrual distress caused by primary dysmenorrhea is associated with physical and psychological symptoms-before, after, and during menstruation. Leventhal's self-regulation educational model is based on the cognitive and emotional experiences of threat responses to symptoms and relates to coping responses. This study aims to investigate the effect of the implementation of a psychoeducational intervention, based on the self-regulation model of menstrual distress in adolescents.

Methods/design: In this randomized controlled trial, 120 adolescent girls with moderate to severe menstrual pain (based on visual analog scale (VAS) ≥ 4) from twelve randomly selected high schools in Qazvin City will be enrolled in the study and will be randomly assigned to either a 3-session psychoeducational intervention (n = 60) or control (n = 60) groups. The sessions will be between 60 and 90 min apiece, and they will run for three consecutive weeks (one session per week). The data collection tools will include questionnaire eliciting menstrual information and demographics, the VAS, the Moos Menstrual Distress Questionnaire, and the illness perception questionnaire. One month prior to the intervention, both groups will participate in an initial assessment to assess the severity of their pain and level of menstrual distress. Finally, all questionnaires will be completed for three consecutive months after the intervention is completed.

Discussion: It is anticipated that findings of this study will provide evidence for the effectiveness of the Leventhal self-regulation model. Implications for improved practice, understanding, and treatment for menstrual distress may also arise.

Ethical Considerations: The research protocol will be reviewed by the ethics committee, which is affiliated with the Qazvin University of Medical Sciences (Decree code: IR.QUMS.REC.1398.043).

Trial Registration: IRCT20190625044002N1 . Registration date: 2019-09-03.
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http://dx.doi.org/10.1186/s13063-020-04629-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450926PMC
August 2020

Death anxiety in patients with cancer: A systematic review and meta-analysis.

Eur J Oncol Nurs 2020 Oct 2;48:101803. Epub 2020 Aug 2.

Social Determinants of Health Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran. Electronic address:

Purpose: The present study was designed to estimate the rate of death anxiety and the factors influencing its occurrence in cancer patients using a systematic review and meta-analysis approach.

Method: Five academic databases, including Scopus, PubMed, Science Direct, Embase, and ProQuest, were searched systematically from inception until the end of January 2020. Observational studies that reported mean score of death anxiety in cancer patients, regardless of language restriction, were eligible to be included. The Newcastle-Ottawa Scale was used to assess the methodological quality of included studies. A meta-analysis was performed using a random effect model with the DerSimonian and Laird weighted method. Publication bias was assessed using the funnel plot and Begg's and Egger's tests.

Results: The estimated pooled mean for death anxiety among cancer patients was 6.84 (CI = [5.98, 7.69]). Publication bias and small study effects were not detected. Subgroup analysis and a meta-regression showed that the estimated pooled mean was higher in Asian studies (M = 7.57) compared to European (M = 6.47) and North American studies (M = 5.57); in breast cancer patients (M = 9.68) compared to studies examining different types of cancers; in female only participants (M = 7.37) compared to studies with both genders (M = 6.60); and in married participants compared to non-married participants.

Conclusions: The findings suggest that death anxiety in patients with cancer is moderate and is influenced by socio-demographic factors such as region, type of cancer, sex, and marital status.
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http://dx.doi.org/10.1016/j.ejon.2020.101803DOI Listing
October 2020

Extra-Peritoneal versus Trans-Peritoneal Open Radical Cystectomy - Comparison of Two Techniques in Early Post-Operative Complications.

Urol J 2020 08 22. Epub 2020 Aug 22.

Department of Urology, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences; Tehran/ Iran.

Purpose: The conventional Trans-Peritoneal Radical Cystectomy (TPRC) harbors numerous postoperative complications, the most prevalent of which are Gastrointestinal (GI) problems. To reduce these morbidities we introduced our own version of extra-peritoneal approach and compared it with the conventional method.  Materials and Methods: In a cross-sectional observational retrospective design, eligible bladder cancer patients whom underwent Extra-Peritoneal Radical Cystectomy (EPRC) or TPRC in our center, were considered for this study and were compared for early post-operative complications .

Results: Ninety nine patients in TPRC and 81 in EPRC were compared. The two techniques differed in their mean operation time (298.2±37.8 min TPRC vs. 262.8±37.2 min EPRC , P: 0.001). Early GI complications were lower in EPRC groups, including oral intake intolerance ( 21 vs. 8, P: 0.04), ileus (19 vs. 8, P: 0.04),  intestinal obstruction (3 vs. 0, P: 0.04) and anastomosis leak (8 vs. 1, P: 0.01). Urine leak (14 vs.7 , P: 0.02) and wound related complications (19 vs. 6 , P: 0.02) also favored EPRC group.

Conclusion: The extra-peritoneal technique is beneficial in reducing the post operative morbidity, especially the more prevalent GI complications. This approach is functionally safe and allows preservation of the peritoneal integrity.
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http://dx.doi.org/10.22037/uj.v16i7.6147DOI Listing
August 2020

Development of A Loop-Mediated Isothermal Amplification (LAMP) Assay for Detection of Relapsing Fever Borreliae.

J Arthropod Borne Dis 2020 Mar 31;14(1):47-55. Epub 2020 Mar 31.

Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran.

Background: This study aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of tick-borne relapsing fever in resource-limited areas.

Methods: A set of six primers were designed based on the conserved regions of the Glycerophosphodiester phosphodiesterase () gene of species. For sensitivity assay, serial dilutions of a recombinant plasmid containing a 219bp sequence of the glpQ were prepared and used as the template DNA. The LAMP reactions containing the six primers and the reagents required for amplification were incubated at 60-65 °C for 60min in a Loopamp real-time turbidimeter. For the specificity test, DNA from 14 other bacteria were included in the assays, and double-distilled water was used as the negative control. Also, DNA from dried blood spots (DBSs) of spirochetemic mice, and blood samples from relapsing fever-suspected patients were examined by the LAMP along a -specific nested PCR that targets the IGS region.

Results: The LAMP detected as low as 90 copies in reactions. The primers reacted with DNA from DBS of spirochetemic mice showing spirochete concentrations of ≤ one per a 1000X microscopic field. In clinical samples, the LAMP assay showed a higher sensitivity compared to nested-PCR. The LAMP specificity was 100%, as the primers did not react with other bacteria DNA.

Conclusion: The high sensitivity and specificity of the test, along with the simplicity of the DNA extraction procedure, make the LAMP a reliable and adaptable tool for the diagnosis of tick-borne relapsing fever in rural endemic areas.
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http://dx.doi.org/10.18502/jad.v14i1.2703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7382693PMC
March 2020

Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.

Int J Biol Macromol 2020 Dec 1;164:1501-1509. Epub 2020 Aug 1.

Department of Microbiology, Faculty of Medicine and Medical Biotechnology Research Center, AJA University of Medical Sciences, Tehran, Iran.

This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage. The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time. The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins. All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples. The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins. As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.07.215DOI Listing
December 2020

Culture-proven endogenous endophthalmitis: microbiological and clinical survey.

Int Ophthalmol 2020 Dec 2;40(12):3521-3528. Epub 2020 Aug 2.

Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, 1336616351, Tehran, Iran.

Purpose: To report causative organisms, visual outcomes, and associated medical conditions in endogenous endophthalmitis (EE).

Methods: Microbiological and medical reports of patients with culture-proven EE referred to Farabi Eye Hospital, Tehran, Iran, from January 2008 to January 2018 were reviewed.

Results: A total of 144 cases (171 eyes) were included in the present study. Candida albicans (30.6%) and Staphylococcus aureus (27.1%) were the most prevalent microbial causes. Diabetes mellitus (43.8%) and IV drug abuse (10.4%) were the leading associated systemic conditions. Age of the patients (P = 0.002) and presenting corrected distance visual acuity (CDVA) (P < 0.001) were correlated with postoperative CDVA. Patients with bilateral involvement had significantly lower mean logMAR (P = 0.002), and patients with hypopyon had significantly higher mean logMAR (P = 0.006). Eyes with gram-negative bacillus cultures had a significantly worse logMAR than eyes with gram-positive cocci (P = 0.009).

Conclusion: Candida albicans and S. aureus were the most common causes of EE, and diabetes mellitus was the most common associated medical condition. EE is associated with poor visual outcome, especially in older patients, those with worse visual acuity at presentation, and those with positive cultures for gram-negative bacilli.
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http://dx.doi.org/10.1007/s10792-020-01540-zDOI Listing
December 2020

Open globe injuries in children under 7 years referred to a tertiary center in Iran from 2006 to 2016.

Eye (Lond) 2020 Jun 22. Epub 2020 Jun 22.

Ocular Trauma and Emergency Department, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: Open globe injuries are one of the most common causes of monocular blindness in many countries. The impact of this traumatic event on patients' quality of life and health is considerable. This study describes the characteristics of open globe injury in children younger than 7 years.

Methods: Six hundred and twenty-two patients were included in this retrospective study. Patients under 7 years with open globe injury were included.

Results: Among 622 patients, 403 (64.8%) were male and 219 (35.2%) were female. The type of material that caused the open globe injury was metallic material in 355 (57.1%) cases, wood in 89 (14.3%) cases, glass in 87 (14%) cases, plastic in 30 (4.8%) cases and other materials in 61 (9.8%) cases. The time between the occurrence of ocular trauma to patient admission was <12 hours in 414 (66.6%) patients and between 12 and 24 hours in 101 (16.2%) patients. The time between patient admission to first surgical intervention was <12 hours in 493 (79.3%) patients, between 12 and 24 hours in 88 (14.1%) patients. The rate of traumatic endophthalmitis in our study was 14.3% at presentation. The most common organism was Streptococcus viridans. Traumatic endophthalmitis was significantly related to the male gender, the time between the occurrence of ocular trauma to patient admission, and time between patient admission to first surgical intervention and wooden material (p < 0.001).

Conclusion: This study showed that open globe injuries were more prevalent in boys older than four years. The earlier patient's referral was associated with a lower rate of endophthalmitis. The most common cause of traumatic endophthalmitis in pre-school children was S. viridans.
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http://dx.doi.org/10.1038/s41433-020-1023-9DOI Listing
June 2020

Validity and Reliability of the Flourishing Scale in a Sample of Older Adults in Iran.

Clin Interv Aging 2020 18;15:673-681. Epub 2020 May 18.

Social Determinants of Health Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.

Background: Flourishing is related to positive outcomes for physical and mental health, as well as overall wellbeing. The aim of the present study was to determine the validity and reliability of the Flourishing Scale (FS) among a sample of older participants in Iran.

Methods: In this validation study, 300 older people were selected by cluster sampling method. Data were collected using a checklist for demographic characteristics, the FS, and the Oxford happiness questionnaire. The validity (face, content, convergent, and construct) and reliability (Cronbach's alpha, test-retest) of the FS was evaluated in this cross-sectional study.

Results: The exploratory factor analysis demonstrated a one-dimensional structure consisting of 8 items with an eigenvalue of 3.583. The model had a good fit [χ = 52.983, p < 0.001, χ/df = 2.944, GFI = 0.958, CFI = 0.915, IFI = 0.949, TLI = 0.919, AGFI = 0.948, and RMSEA = 0.081, SRMR = 0.086] with all factor loadings greater than 0.5 and statistically significant. A test of concurrent validity showed a direct and significant association between the FS and the Oxford happiness questionnaire ( = 0.647, < 0.001). The results of the reliability tests confirmed that the values of Cronbach's alpha coefficient (0.819) and test-retest (0.821) were acceptable.

Conclusion: The Persian version of the FS demonstrated suitable validity and reliability among a sample of older participants.
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http://dx.doi.org/10.2147/CIA.S251067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244746PMC
November 2020

Effects of different graphene-based nanomaterials as elicitors on growth and ganoderic acid production by Ganoderma lucidum.

Biotechnol Prog 2020 09 15;36(5):e3027. Epub 2020 Jun 15.

Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.

Graphene-based nanomaterials (GBNs) have attracted considerable interest nowadays due to their wide range of applications. However, very little attention has been paid to the application of nanomaterials as potential elicitors for production of valuable metabolites. Herein, aiming to earn insight into effects of nanomaterials on secondary metabolite biosynthesis by medicinal fungi, we evaluated the influence of GBNs on growth and production of ganoderic acid (GA) by Ganoderma lucidum in submerged culture. Graphene oxide (GO), reduced graphene oxide (rGO), and rGO/Fe O nanocomposite were synthesized successfully and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy analysis. The prepared nanomaterials were added to the culture of G. lucidum at final concentrations of 50, 100, and 150 mg/L on Day 5. The results showed that the elicitation of G. lucidum with GO and rGO decreased the cell dry weight and GA production slightly, especially in higher concentrations. However, rGO/Fe O nanocomposite not negatively affected cell growth and improved GA production. G. lucidum growth rate responded to elicitation experiments differently and depended on the type of nanomaterials and their concentrations, but almost all GBNs caused an increase in GA content (mg/100 mg dry weight). Also, field emission scanning electron microscopy morphological study showed that under elicitation, mycelia were more condensed and tightly stacked together. The findings from this study may suggest that GBNs in low concentrations could be applied as elicitors to secondary metabolites production from higher fungus, but further environmental, physiological, and biological studies required.
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http://dx.doi.org/10.1002/btpr.3027DOI Listing
September 2020

Accuracy of Swept-Source Optical Coherence Tomography and Ultrasound Biomicroscopy for Evaluation of Posterior Lens Capsule in Traumatic Cataract.

Am J Ophthalmol 2020 08 2;216:55-58. Epub 2020 Apr 2.

Ocular Trauma and Emergency Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To assess the efficacy of swept-source optical coherence tomography (SS-OCT) and ultrasound biomicroscopy (UBM) in detecting posterior capsule (PC) defect in patients with traumatic cataract.

Design: Observational case-series.

Methods: Sixty-seven eyes from 67 patients, with traumatic cataract severe enough to prevent slit lamp evaluation of the PC, were included in a simple sequence without randomization. Patients underwent both 50-MHz UBM and SS-OCT evaluation of the PC by different operators. Cataract surgery was then performed using a single technique.

Results: Sixty-seven eyes from 67 patients including 60 men and 7 women were studied. The mean age was 34 ± 14 years and the mean logarithm of minimal angle of resolution of visual acuity was 1.89 ± 0.71. The calculated sensitivity, specificity, and accuracy values for SS-OCT were 96.8% (95% confidence interval [CI] 83.81-99.43), 66.7% (95% CI 48.78-80.77), and 82% (95% CI 70.53-89.62), respectively. For UBM, sensitivity, specificity, and accuracy values were 82.6% (95% CI 62.86-93.02), 57.9% (95% CI 36.28-76.86), and 71.4% (95% CI 56.43-82.83), respectively. Positive predictive and negative predictive values for SS-OCT were 75% (95% CI 59.81-85.81) and 95.2% (95% CI 77.33-99.15) and for UBM were 70.4% (95% CI 51.52-84.15) and 73.3% (95% CI 48.05-89.1), respectively.

Conclusion: Although both imaging techniques are effective, SS-OCT appears to be at least comparable, or superior in special circumstances, to UBM in detecting preoperative posttraumatic PC rupture. We recommend preoperative assessment of all traumatic cataracts with SS-OCT as a part of surgical planning.
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http://dx.doi.org/10.1016/j.ajo.2020.03.030DOI Listing
August 2020

Complications of intrastromal bevacizumab injection in lamellar keratoplasty.

Int J Ophthalmol 2020 18;13(2):356-358. Epub 2020 Feb 18.

Ocular Trauma and Emergency Department, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran 1336616351, Iran.

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http://dx.doi.org/10.18240/ijo.2020.02.22DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7013780PMC
February 2020

Predictors of Quality of Life in Patients with Heart Disease.

J Relig Health 2020 Aug;59(4):2135-2148

Educational Psychology and Inclusive Education, Faculty of Education, Monash University and The Centre for Positive Psychology, The Melbourne Graduate School of Education, The University of Melbourne, Parkville, Australia.

Individuals with heart disease have been found to have more negative psychological and physical effects that impact their quality of life (QoL) than the general population. Spiritual well-being is considered a protective factor associated with QoL in people with heart disease. Therefore, the current research seeks to evaluate whether sociodemographic factors and spiritual well-being predict QoL among patients with heart disease. A total of 500 patients who were selected through a convenient sampling method from an Iranian hospital participated in this descriptive-correlational study. Data were collected using the McGill QoL Questionnaire, the Spiritual Well-being Scale, and demographic variables. The data analysis included descriptive and inferential statistics powered by SPSS (v. 23). Following multivariate analyses, findings revealed that those participants with their main source of income derived from family or a government pension and with College or intermediate educational levels were more likely to have higher QoL. Those participants with average or poor socioeconomic status reported higher QoL than those who were more affluent. Furthermore, younger patients (- 0.2, 95% CI - 0.3 to - 0.003, p = 0.016) and those with higher social support (0.7, 95% CI 0.2 to 1.3, p = 0.006) and spiritual well-being (0.2, 95% CI 0.1 to 0.3, p < 0.001) had significantly better QoL. In the current study, spiritual well-being and social support led to reduced negative psychological sequelae and improved QoL in cardiac patients.
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http://dx.doi.org/10.1007/s10943-019-00968-7DOI Listing
August 2020

Pachydermoperiostosis: A clinicopathological description.

J Curr Ophthalmol 2019 Dec 29;31(4):450-453. Epub 2019 Mar 29.

Pathology Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To report a case of pachydermoperiostosis (PDP) and a review of the literature.

Methods: A 32-year-old man was referred to our clinic with bilateral eyelid swelling and blepharoptosis. On examination, marked blepharoptosis was noted, and his eyelids were found to be floppy. Systemic examination was significant for clubbing of digits, coarse acromegalic facial features, and furrowing and oiliness of the skin of scalp and forehead.

Results: The patient was diagnosed as a case of PDP. On the brain MRI, the pituitary gland was enlarged, and the border of clivus was irregular. Pituitary and thyroid hormone levels were normal. He underwent bilateral lateral tarsal strip (LTS) procedure to address the eyelid laxity. Histopathologic examination revealed marked sebaceous gland hyperplasia with mucin deposition in the dermis.

Conclusion: Floppy eyelid syndrome, clubbing, and acromegaloid face are main features that could lead to the diagnosis of PDP.
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http://dx.doi.org/10.1016/j.joco.2019.03.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896461PMC
December 2019

Shifting to very early endoscopic DCR in acute suppurative dacryocystitis.

Eye (Lond) 2020 09 10;34(9):1648-1653. Epub 2019 Dec 10.

Ophthalmology Department, Farabi Hospital, Eye Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: We aimed to show the outcome of very early endoscopic dacryocystorhinostomy (VE-EDCR) in a routine pool of patients with acute dacryocystitis (AD) and abscess formation compared with the standard late external dacryocystorhinostomy L-ExDCR.

Methods: This was a prospective nonrandomized comparative study conducted from June 2013 to March 2016. Patients with AD and abscess formation were referred to our oculo-facial clinic in a university-based hospital. All patients received systemic antibiotics and were assigned to either of treatment groups. Patients in group 1 underwent late external transcutaneous DCR (L-ExDCR) and group 2 underwent EDCR within 3 days after first visit, named VE-EDCR. Primary outcome measure was success of surgery.

Results: Forty-one eyes of 41 patients with acute suppurative AD, were included from June 2013 to March 2016. Twenty-two patients underwent VE-EDCR and 19 underwent L-ExDCR. Mean age of patients was 43.41 (SD = 19.84, range 14-98) years. Mean follow-up was 14 (SD = 2.4) months. Anatomic, functional, and overall success in L-ExDCR and VE-EDCR groups were (89.5 and 86.4%, p = 0.99) (89.5% and 86.4%, p = 0.99) (89.5% and 81.8%, p = 0.66) respectively. Mean duration of cellulitis in VE-EDCR and L-ExDCR were 8.00 (SD = 4.63) and 16.11 (SD = 11.58) days, respectively (p = 0.027). No remarkable adverse event was found.

Conclusions: Success of very early endonasal endoscopic DCR is comparable with the traditional late external DCR. Duration of cellulitis is shorter in VE-EDCR. This therapeutic approach can be considered in patients with acute suppurative dacryocystitis.
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http://dx.doi.org/10.1038/s41433-019-0734-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609300PMC
September 2020

The use of in vivo confocal microscopy to track treatment success in fungal keratitis and to differentiate between Fusarium and Aspergillus keratitis.

Int Ophthalmol 2020 Feb 7;40(2):483-491. Epub 2019 Nov 7.

Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To evaluate the usefulness of serial in vivo confocal microscopy (IVCM) examinations to measure hyphal density for monitoring the treatment success among patients with fungal keratitis, and to compare the hyphal diameter as well as branching angle as a way of differentiation between Aspergillus and Fusarium species observed in IVCM.

Study Design: Prospective nonrandomized study.

Patients And Methods: The study was conducted from February 2015 to September 2016. Hyphal diameter, density and branching angle measurements were performed using IVCM at admission and on a weekly basis for at least 2 weeks after the start of treatment.

Results: During the period of study, 65 patients with culture-confirmed fungal keratitis were recruited. Of them, 40 were culture-positive for Fusarium spp. and 25 patients for Aspergillus spp. Before the start of treatment, the mean branching angle did not differ between the two species and the mean hyphal diameter was statistically higher for Aspergillus spp. (p = 0.029). Two weeks after the start of treatment, the mean hyphal diameter was statistically lower (p < 0.001) in the treatment failure group. Also the hyphal density significantly decreased with successful treatment (p < 0.05).

Conclusion: Decreasing hyphal density in serial IVCMs might be used as an indicator to predict the successful response of fungal ulcers to treatment. Branching angle is not different between Aspergillus and Fusarium keratitis. The mean hyphal diameter is significantly lower in the treatment failure group.
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http://dx.doi.org/10.1007/s10792-019-01209-2DOI Listing
February 2020

Serological and molecular approaches in clinical diagnosis of ocular toxoplasmosis in Iran.

Med J Islam Repub Iran 2019 12;33:82. Epub 2019 Aug 12.

Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

) is the most common parasite that can lead to a disease called toxoplasmosis. In this study, serological and molecular complementary tests have been conducted to detect or diagnose this parasite. A total of 71 patients with clinical symptoms of ocular toxoplasmosis and 20 patients with other ocular infections were evaluated. Serum and buffy coat samples were collected and tested using enzyme-linked immunosorbent assay (ELISA) and nested polymerase chain reaction (nPCR) assessments. Superficial B1 gene was evaluated in PCR. The ocular toxoplasmosis patients were followed-up 2 weeks after the first sampling and 4 weeks following the first laboratory testing. The main outcome measures were the efficiency of the diagnostic procedure and positive and negative predictive values (PPV and NPV). Overall, of the samples, 69% were PCR+, IgG+, and IgM-, and 4.2% showed PCR+, IgG+, and IgM+. In the first follow-up, after 2 weeks, from the 41 referred patients, 29 (70%) showed PCR+, IgG+, and IgM-, which confirmed the results of the first sampling. In the second follow-up, 9 (47%) patients were PCR+, IgG+, and IgM-. A correlation was observed between the first referral and the follow-ups. Also, from 71 patients, diagnosed clinically as ocular toxoplasmosis, the disease was confirmed in 73.2% and 26.8% of those suffering from other ocular infections. Of the 20 control group samples, 55% showed PCR-, IgG+, and IgM-. The sensitivity, specificity, negative and positive predictive values, and negative and positive likelihoods were analyzed for IgG and IgM antibodies and for PCR using ELISA method. As the ophthalmologic signs of may be mimicked by other infections, clinical methods may be complemented by laboratory approaches for a definite diagnosis. This would assist clinicians to achieve timely diagnosis and successful therapy and to control the infection.
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http://dx.doi.org/10.34171/mjiri.33.82DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825372PMC
August 2019

Infectious keratitis after pterygium surgery.

Eye (Lond) 2020 05 24;34(5):986-988. Epub 2019 Oct 24.

Ocular Trauma and Emergency Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1038/s41433-019-0639-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7182561PMC
May 2020