Was born on 2nd March, 1980 in Dhanbari, Tangail, Bangladesh. I am an educator of biotechnology subjects at Shere-e-Bangla Agricultural University, Dhaka, Bangladesh and doing research in the same field. Currently I am on study-leave and thereby, a PhD student of Marine Biotechnology, University of Science & Technology (UST), South Korea and working as Research Assistant at Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology (KIOST), South Korea. I feel happy being father of a daughter and a son!!
Primary Affiliation: Sher-e-Bangla Agricultural University -Bangladesh - Dhaka , Bangladesh
Stinging catfish (Heteropneustes fossilis) is an important aquaculture fish species in Bangladesh with
high nutritive value. Three microsatellite loci (Cba02, Cba19 and Cba20) were tested to study the genetic
variation in this species. Samples were collected from two natural populations namely Mohongonj and
Narsingdi and from a hatchery population located in Mymensingh of Bangladesh. All the three loci were found
to be polymorphic (P ) in the three studied populations. The average observed heterozygosity (H ) value was 95 o
the highest in Narsingdi population (0.80) followed by the Hatchery (0.70) and the Mohongonj population
(0.67). Only Mohongonj population was significantly deviated from Hardy-Weinberg Equilibrium in one locus.
The population differentiation value (F ) between all the population pairs was insignificant. The highest ST
genetic distance value (D = 0.47) was found between the Mohongonj and Hatchery populations while the
smallest value (D = 0.34) was found between the Mohongonj and Narsingdi as well as Narsingdi and Hatchery
populations. The UPGMA dendrogram based on genetic distance resulted in two major clusters: Hatchery stock
alone is in one cluster and the remaining two natural stocks are in other cluster. The study also revealed that
the Clarias batrachus microsatellites could be effectively used in the assessment of genetic structure of the
stinging catfish H. fossilis
DNA fingerprinting and genetic diversity analysis helps direct selective breeding and conservation of plant species. Since simple sequence repeats (SSR) markers are co-dominant, they can predict level of genetic diversity and thereby protect plant genetic resources of a region. Keeping the aforesaid rationale in mind, we worked on molecular characterization of eight cotton varieties in Bangladesh using simple sequence repeat (SSR) or microsatellite DNA markers. All the three microsatellite DNA markers were found to be polymorphic, extracting a total of eight alleles with an average of 2.67 alleles per locus in the present study. Allele sizes were as 149-155 bp, 178-198 bp and 140-202 bp for the loci BNL1551, BNL1721 and BNL2960, respectively. Polymorphism Information Content (PIC) values were ranged from 0.469 to 0.531. UPGMA dendrogram separated 8 varieties of cotton into two clusters. One cluster contained six varieties CB-1, CB-2, CB-3, CB-7, CB-9 and CB-10 while other two varieties CB-5 and HC 1 formed another cluster. The findings of this study would provide a useful guide for selecting specific germplasm with distinct genetic background for diversifying cotton breeding program in Bangladesh.
Microsatellite DNA marker analysis was carried out to assess the population genetic structure of an endangered carp, Labeo calbasu, collected from three different stocks; the Jamuna River, the Halda River and a Hatchery. Four heterologous microsatellite loci (Lr12, Lr14b, Lr21 and Lr24) identified from rohu (Labeo rohita) were analyzed to test the genetic variability of the target kalibaus stocks. The maximum number of alleles observed in loci Lr12, Lr14b, Lr21 and Lr24 were 10, 7, 8 and 6, respectively. The loci were found to be polymorphic (
Microsatellite DNA markers have been increasingly used in genetic diversity studies. The present study reports on the
characterization of genetic variation and differentiation in four different natural populations of the stinging catfish,
Heteropneustes fossilis (Bloch), in Bangladesh, viz., Mymensingh, Netrakona, Narsingdi and Rangpur, using cross-species
microsatelllite DNA markers developed from the walking catfish, Clarias batrachus. Eighteen polymorphic alleles were
found in the 128 diploid individuals (32 from each population), with nine alleles at each of the two loci analyzed. The
Netrakona and Rangpur population deviated from the Hardy-Weinberg proportion at one locus. The population
differentiation (FST) value between the Narsingdi and Netrakona population was found to be insignificant, while the values
between all the other population pairs were found to be significant. The genetic distance values ranged between 0.165 and
0.626. The UPGMA dendrogram based on genetic distance resulted in two clusters: the Mymensingh population alone was
in one cluster and the three other populations in the second cluster. This study revealed a fairly high level of genetic
variation in the microsatellite loci within and between the four populations, and identified existence of distinct population
groups of H. fossilis.
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