Publications by authors named "Mohammad Ali"

1,000 Publications

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Oculoplastic Abstracts.

Ophthalmic Plast Reconstr Surg 2022 May-Jun 01;38(3):313-314. Epub 2022 May 11.

∥Ain Shams University, Cairo, Egypt.

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http://dx.doi.org/10.1097/IOP.0000000000002183DOI Listing
May 2022

Disability, physical activity, and health-related quality of life in Australian adults: An investigation using 19 waves of a longitudinal cohort.

PLoS One 2022 12;17(5):e0268304. Epub 2022 May 12.

School of Public Health, University of Technology Sydney, Sydney, Australia.

Background: Any form of long-term physical or mental impairment might negatively influence health-related quality of life (HRQoL). HRQoL, as an independent concept, covers a wide range of characteristics that includes physical, mental, social, and spiritual functions. People with disabilities are continuously exposed to multiple barriers that deteriorate their HRQoL. It also creates impairment in performing physical activities. However, experts opine regular physical exercise as an intervention to help disabled people. This research aims to investigate the association between disability and physical activity with HRQoL among the adult population in Australia.

Design: A retrospective cohort study.

Methods: This study utilized the most recent 19 waves of data (2002-2020) from the nationally representative Household, Income and Labour Dynamics in Australia (HILDA) survey. Component summary scores such as physical component summary (PCS) and mental component summary (MCS), and SF-6D utility scores were utilized to measure HRQoL. Random-effects GLS regression technique was fitted to estimate the association between disability and physical activity with HRQoL, after adjusting for a range of socio-demographic and health-related characteristics.

Results: Disability was negatively associated with the PCS (-5.95), MCS (-2.70) and SF-6D (-0.060) compared with non-disabled counterparts. However, respondents engaged in the recommended level of physical activity had substantial gain in PCS (b = 0.96), MCS (1.57), and SF-6D (0.021) scores. Besides, the results showed that performing the recommended level of physical activity in the presence of disability has lessen the negative effect of disability/ positive moderating effect of physical activity on PCS, MCS, and SF-6D scores by 1.84 points, 0.82 points, and 0.013 percentage points, respectively.

Conclusion: This study found an inverse association between disability and HRQoL among Australian adults. However, physical activity was associated with improved HRQoL. Therefore, public health interventions, such as the orientation of physical activities, have a higher potential to dwindle the burden regarding HRQoL.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0268304PLOS
May 2022

Routine punctoplasty: isn't it time we preserved the integrity of the punctum?

Orbit 2022 May 2:1-6. Epub 2022 May 2.

Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA.

Punctoplasty is a common procedure in the management of punctal stenosis and was first described by Sir William Bowman in 1853. Several types of punctoplasty are described, and include 1-snip, 2-snip, triangular 3-snip, rectangular 3-snip and 4-snip procedures. The understanding of the anatomy and physiology of the punctum has greatly improved in the last decade aided by electron microscopic and molecular techniques. The use of minimally invasive modalities for primary cases is on the rise with successful preservation of the physiological functions of the punctum and canaliculus. The paper reviews the current anatomical and physiological evidence to present an alternative perspective. Given our evolving understanding, it may be time to debate the practice of performing routine punctoplasty.
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http://dx.doi.org/10.1080/01676830.2022.2055087DOI Listing
May 2022

Microbial Metagenomics of the Extubated Lacrimal Stents Following Dacryocystorhinostomy: The Lacriome Paper 4.

Ophthalmic Plast Reconstr Surg 2022 Apr 25. Epub 2022 Apr 25.

Govindram Seksaria Institute of Dacryology, LV Prasad Eye Institute, Hyderabad, India.

Purpose: To study the metagenome of the microbes present on the extubated lacrimal stents following a dacryocystorhinostomy.

Methods: A prospective study was performed on 10 consecutive extubated lacrimal stents obtained for the metagenomic analysis from the patients following an endoscopic dacryocystorhinostomy. The stents were extubated at 4 weeks postoperatively under endoscopic guidance and immediately transported on ice to the laboratory. Following DNA extraction and library preparation, a whole shotgun metagenome sequencing was performed on the Illumina platform. The downstream processing and bioinformatics of the samples were performed using multiple software packaged in SqueezeMeta pipeline or MG-RAST pipeline.

Results: The taxonomic hit distribution across the stent samples showed that bacteria were the most common isolates (mean, 69.70%), followed by viruses (mean, 0.02%) and archaea (0.003%). The 3 major phyla identified were Firmicutes, Actinobacteria, and Proteobacteria. The prevalent organisms include Pseudomonas aeruginosa, Staphylococcus aureus, Corynebacterium accolens, Dolosigranulum pigrum, Citrobacter koserii, Staphylococcus epidermidis, E. coli, and Hemophilus influenza. The functional subsystem profiling demonstrated microbial genes associated with metabolism, cellular, and information processing. The functional subsystem categories were metabolism involving carbohydrates, amino acids, DNA and RNA, cell wall or cell capsule biogenesis, membrane transport, virulence, and defense mechanisms.

Conclusions: The present study is the first whole metagenome sequencing of the microbes isolated from the extubated lacrimal stents. The stents harbor diverse microbial communities with distinct ecosystem dynamics. Further studies on microbes-host interactions in the early postoperative period would provide valuable insights.
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http://dx.doi.org/10.1097/IOP.0000000000002202DOI Listing
April 2022

Lacrimal Sac Reconstruction Using a Nasal Mucosal Graft.

Ophthalmic Plast Reconstr Surg 2022 Apr 25. Epub 2022 Apr 25.

Govindram Seksaria Institute of Dacryology, L.V. Prasad Eye Institute, Hyderabad, India.

Iatrogenic lacrimal drainage trauma during surgery is not common. It has been reported during orbital surgeries, medial maxillectomies, post-trauma repairs, excision of adherent lesions in the vicinity, and facial reconstructive procedures. The present case reports a focal loss of the lacrimal sac wall during the excision of an intricate dermoid cyst. The defect was sealed by a nasal mucosal graft harvested from the floor of the ipsilateral nasal cavity. Postoperatively, the epiphora was resolved, and a CT-dacryocystography confirmed the anatomical integrity of the reconstructed lacrimal sac.
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http://dx.doi.org/10.1097/IOP.0000000000002189DOI Listing
April 2022

Histopathological Evidence of Canalicular Agenesis in Patients With Punctal Agenesis: Punctum Update Study 2.

Ophthalmic Plast Reconstr Surg 2022 Apr 25. Epub 2022 Apr 25.

Govindram Seksaria Institute of Dacryology, L.V.Prasad Eye Institute, Hyderabad, India.

Purpose: The purpose of this study was to histologically assess the presence and extent of the canalicular tissue in cases of punctal agenesis.

Methods: A prospective, interventional study was performed on 10 consecutive cases of punctal agenesis over a study period of 6 months. Exclusion criteria included cases with punctal dysgenesis or incomplete punctal canalization, secondary/acquired cases of punctal effacement, or any prior interventions. Following the exploration, the entire specimen of the tissue (length = 8 mm and depth = 3 mm) was sent for histopathological analysis to ascertain the presence and extent of the canalicular tissue.

Results: The mean age of the patients at the time of exploration was 7.5 years (range 6-10 years). Histopathological analysis showed tissue surfaces lined with stratified squamous epithelium with occasional interspersed goblets cells (conjunctival epithelium), a few chronic inflammatory cells, and muscle fragments. Epithelium suggestive of proximal lacrimal drainage system was absent. Canalicular tissues were found to be absent in all cases of punctal agenesis.

Conclusion: The present series did not find any histologic evidence of canalicular tissues in cases of punctal agenesis. The findings of this study do not support retrograde approaches in cases of punctal agenesis.
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http://dx.doi.org/10.1097/IOP.0000000000002188DOI Listing
April 2022

Utilization of Artificial Intelligence in Disease Prevention: Diagnosis, Treatment, and Implications for the Healthcare Workforce.

Healthcare (Basel) 2022 Mar 24;10(4). Epub 2022 Mar 24.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.

Artificial intelligence (AI) has been described as one of the extremely effective and promising scientific tools available to mankind. AI and its associated innovations are becoming more popular in industry and culture, and they are starting to show up in healthcare. Numerous facets of healthcare, as well as regulatory procedures within providers, payers, and pharmaceutical companies, may be transformed by these innovations. As a result, the purpose of this review is to identify the potential machine learning applications in the field of infectious diseases and the general healthcare system. The literature on this topic was extracted from various databases, such as Google, Google Scholar, Pubmed, Scopus, and Web of Science. The articles having important information were selected for this review. The most challenging task for AI in such healthcare sectors is to sustain its adoption in daily clinical practice, regardless of whether the programs are scalable enough to be useful. Based on the summarized data, it has been concluded that AI can assist healthcare staff in expanding their knowledge, allowing them to spend more time providing direct patient care and reducing weariness. Overall, we might conclude that the future of "conventional medicine" is closer than we realize, with patients seeing a computer first and subsequently a doctor.
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http://dx.doi.org/10.3390/healthcare10040608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9026833PMC
March 2022

The Cry of the Third Eye: Exceptionally Rare Location of a Post-Traumatic Acquired Lacrimal Fistula.

Ocul Immunol Inflamm 2022 Apr 20:1-3. Epub 2022 Apr 20.

Govindram Seksaria Institute of Dacryology, L.V. Prasad Eye Institute, Hyderabad, India.

Purpose: To report an unusual case of an ectopic lacrimal fistula in a setting of a naso-orbito-ethmoid fracture.

Case Report: The present case demonstrates a right-sided ectopic lacrimal fistula just below the glabella on the contralateral side on the outer edge of the midline. CT-Dacryocystography demonstrated a large dilated right lacrimal sac with a partly sequestered fundus traversing through the frontal process of the maxilla and frontal bone, close to the skull base and communicating with the fistula. The connection of the fistula with the sac was severed at the superior-most point, and the long fistulous tract was removed using external and endoscopic approaches, followed by endocautery and subsequent dacryocystorhinostomy. Post-operatively, the fistulous opening healed, ostium score was FICI grade 5, and the patient was asymptomatic.

Conclusion: The case demonstrates multiple surgical challenges while managing an ectopic fistula with a long fistulous tract traversing several bony structures.
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http://dx.doi.org/10.1080/09273948.2022.2060262DOI Listing
April 2022

Multifocal Punctal Rim Papilloma Mimicking a Canalicular Origin.

Ophthalmic Plast Reconstr Surg 2022 Apr 13. Epub 2022 Apr 13.

Govindram Seksaria Institute of Dacryology, L.V. Prasad Eye Institute, Hyderabad, India.

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http://dx.doi.org/10.1097/IOP.0000000000002204DOI Listing
April 2022

Endoscopic Evidence of the Punctum-Canalicular Junction.

Ophthalmic Plast Reconstr Surg 2022 Apr 13. Epub 2022 Apr 13.

'Govindram Seksaria Institute of Dacryology', L.V. Prasad Eye Institute, Hyderabad, India.

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http://dx.doi.org/10.1097/IOP.0000000000002193DOI Listing
April 2022

Comment on Rochmah et al. Economic Burden of Stroke Disease: A Systematic Review. 2021, , 7552.

Int J Environ Res Public Health 2022 03 30;19(7). Epub 2022 Mar 30.

Department of Information Engineering, University of Brescia, 25123 Brescia, Italy.

We have read with great interest the recently published article titled "Economic Burden of Stroke Disease: A Systematic Review" by Rochmah and colleagues [...].
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http://dx.doi.org/10.3390/ijerph19074095DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8998598PMC
March 2022

Characteristics of Conventional and Microwave Sintered Iron Ore Preform.

Materials (Basel) 2022 Apr 4;15(7). Epub 2022 Apr 4.

Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha 61413, Saudi Arabia.

In this study, compacted hematite (FeO) preforms were made and sintered at various temperatures, such as 1250 °C and 1300 °C, using both conventional and microwave sintering methods. The density, porosity, microhardness, cold crushing strength, microphotographs, and X-ray diffraction (XRD) analysis of the sintered preforms were used to evaluate the performance of the two sintering methods. It was found that microwave sintered preforms possessed lesser porosity and higher density than conventionally sintered preforms owing to uniform heating of the powdered ore in microwave sintering method. Furthermore, it was also observed that microwave sintered preforms exhibited relatively higher cold crushing strength and hardness than conventionally sintered preforms. Thus, the overall results revealed that microwave sintering yielded better properties considered in the present study.
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http://dx.doi.org/10.3390/ma15072655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9000582PMC
April 2022

Developing a core outcome set for patient-reported symptom monitoring to reduce hospital admissions for patients with heart failure.

Eur J Cardiovasc Nurs 2022 Apr 11. Epub 2022 Apr 11.

Department of Cardiovascular Sciences, University of Leicester, and NIHR Cardiovascular Biomedical Research Centre, Glenfield Hospital, Leicester, Leicestershire LE5 4PW, UK.

Aims: In patients with heart failure (HF), hospitalization rates are increasing, particularly for non-HF causes and over half may be avoidable. Self-monitoring of symptoms plays a key part in the early identification of deterioration. Our objective was to develop expert consensus for a core outcome set (COS) of symptoms to be monitored by patients, using validated single-item patient-reported outcome measures (PROMs), focused on the key priority of reducing admissions in HF.

Methods And Results: A rigorous COS development process incorporating systematic review, modified e-Delphi and nominal group technique (NGT) methods. Participants included 24 HF patients, 4 carers, 29 HF nurses, and 9 doctors. In three Delphi and NGT rounds, participants rated potential outcomes on their importance before a HF or a non-HF admission using a 5-point Likert scale. Opinion change between rounds was assessed and a two-thirds threshold was used for outcome selection.Item generation using systematic review identified 100 validated single-item PROMs covering 34 symptoms or signs, relevant to admission for people with HF. De-duplication and formal consensus processes, resulted in a COS comprising eight symptoms and signs; shortness of breath, arm or leg swelling, abdomen bloating, palpitations, weight gain, chest pain, anxiety, and overall health. In the NGT, a numerical rating scale was selected as the optimal approach to symptom monitoring.

Conclusion: Recognition of a range of HF-specific and general symptoms, alongside comorbidities, is an important consideration for admission prevention. Further work is needed to validate and integrate the COS in routine care with the aim of facilitating faster identification of clinical deterioration.
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http://dx.doi.org/10.1093/eurjcn/zvac019DOI Listing
April 2022

Work-life balance: choose wisely.

Semin Ophthalmol 2022 Apr 5:1-2. Epub 2022 Apr 5.

L.V. Prasad Eye Institute, Hyderabad, India.

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http://dx.doi.org/10.1080/08820538.2022.2058798DOI Listing
April 2022

Metagenomics of infective canaliculitis: The Lacriome paper 3.

Eur J Ophthalmol 2022 Mar 30:11206721221091646. Epub 2022 Mar 30.

Govindram Seksaria Institute of Dacryology, 28592L.V. Prasad Eye Institute, Hyderabad, India.

Purpose: To study the metagenomics of the microbes isolated from the canaliculus of patients with infective canaliculitis.

Methods: A prospective study was performed on five consecutive canalicular samples obtained for the metagenomic analysis from the patients with infective canaliculitis who underwent non-incisional canalicular curettage at a tertiary care Dacryology service. The canalicular concretions were collected intraoperatively soon after a canalicular curettage and immediately transported on ice to the laboratory. Following DNA extraction and library preparation, a whole shotgun metagenome sequencing was performed on the Illumina™ platform. The downstream processing and bioinformatics of the samples were performed using multiple software packaged in SqueezeMeta™ pipeline or MG-RAST™ pipeline.

Results: The taxonomic hit distribution across the samples showed that bacteria were the most common isolates (mean-80.5%), followed by viruses (mean-0.74%), and archaea (0.01%). The five major phyla identified across the samples of infective canaliculitis were, Fusobacteria, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes. The prevalent organisms include and amongst few others. was noted in all the samples, though it was not the most abundant. The microbial gene mapping and protein prediction demonstrated proteins with known functions to range from 69.91% to 87.09% across the samples. The functional subsystem profiling demonstrated genes associated with carbohydrate, amino acid, and co-enzyme transport and metabolism, cell wall or cell membrane biogenesis, energy production and conversion, transcription, translation, and cellular communications.

Conclusion: This is the first whole metagenome sequencing of infective canaliculitis. Infected canaliculi harbor diverse microbial communities, including bacteria, viruses, and archaea. Functional analysis has provided newer insights into the ecosystem dynamics and strategies of microbial communities.
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http://dx.doi.org/10.1177/11206721221091646DOI Listing
March 2022

The Clinical Practice Adopted by Physiotherapists in Managing Head and Neck Cancer Subjects within the United Arab Emirates: A Survey.

Asian Pac J Cancer Prev 2022 Mar 1;23(3):851-860. Epub 2022 Mar 1.

Department of Physiotherapy, College of Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.

Background: Globally, cancer is one of the main causes of death. Nonetheless, the estimate for Middle Eastern countries is high and rising. Furthermore, there is a paucity of evidence on effective rehabilitation measures for treatment of cancer patients, particularly head and neck cancer patients. As a result, the purpose of this study is to enlist current physical therapy clinical practice adopted by physiotherapists for the assessment and treatment of patients with head and neck cancer in United Arab Emirates.

Methodology: A total of 100 survey questionnaires were sent via e-mail to physical therapists across the United Arab Emirates 's oncology rehabilitation centers for this cross-sectional study. Physical therapists having a valid license and at least one year of experience treating patients with head and neck cancer were included in the study. The collected responses were analyzed using descriptive statistics.

Results: The total questionnaires generated a response rate of 56% of the targeted population. A percentage of 67% of the survey marked "Always or Frequently" by more than 50% of responders. Components aiming at the musculoskeletal system in both assessment and management were used more than those targeting the cardiopulmonary system. Functional capacity and Quality of Life measures were not given as much attention however 50% of responders reported using the Fatigue and Severity Scale (FSS). Mobilization was notably marked in management, especially Upper Extremity Training which was utilized by 86.4% of responders. Educational tips components all ranged around 40-60% except for Breathing Exercise which peaked at 85.7% of the responders.

Discussion And Conclusion: Assessment measures predominantly focused on functional impairment measures of the upper limb, including functions related to mandible, tongue, mouth opening, and neck movement. Treatment patterns on the other hand predominantly centred on functionally related exercises as upper limb training, breathing exercises, and facial muscle exercise.
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http://dx.doi.org/10.31557/APJCP.2022.23.3.851DOI Listing
March 2022

Silver and gold nanoparticles induced differential antimicrobial potential in calli cultures of Prunella vulgaris.

BMC Chem 2022 Mar 25;16(1):20. Epub 2022 Mar 25.

Université d'Orléans, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, 28000, Chartres, France.

Background: Prunella vulgaris is medicinally important plant containing high-valued chemical metabolites like Prunellin which belong to family Lamiaceae and it is also known as self-heal. In this research, calli culture were exposed to differential ratios of gold (Au) and silver (Ag) nanoparticles (1:1, 1:2, 1:3, 2:1 and 3:1) along with naphthalene acetic acid (2.0 mg NAA) to investigate its antimicrobial potential. A well diffusion method was used for antimicrobial properties.

Results: Here, two concentrations (1 and 2 mg/6 µl) of all treated calli cultures and wild plants were used against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Bacillus atrophaeus, Bacillus subtilis, Agrobacterium tumefaciens, Erwinia caratovora and Candida albicans. Dimethyl sulfoxide (DMSO) and antibiotics were used as negative and positive controls. Here, the calli exposed to gold (Au) nanoparticles (NPs) and 2.0 mg naphthalene acetic acid (NAA) displayed the highest activity (25.7 mm) against Salmonella typhi than other extracts, which was considered the most susceptible species, while Agrobacterium tumefaciens and Candida albicans was the most resistance species. A possible mechanism of calli induced nanoparticles was also investigated for cytoplasmic leakage.

Conclusion: From the above data it is concluded that Prunella vulgaris is medicinally important plant for the development of anti-microbial drugs using nanotechnology and applicable in various pharmaceutical research.
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http://dx.doi.org/10.1186/s13065-022-00816-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8957128PMC
March 2022

The retinal vascular growth rate in babies with retinopathy of prematurity could indicate treatment need.

Indian J Ophthalmol 2022 Apr;70(4):1270-1277

Srimati Kanuri Santhamma Centre for Vitreoretinal Diseases, Cuttack, Odisha, India.

Purpose: To analyze the weekly rate of retinal vascular growth in treatment-naïve babies with various stages of retinopathy of prematurity (ROP) and validate if this could be a predictor of treatment need.

Methods: Retrospective review of medical charts and retinal images of babies with various stages of ROP. The images were enhanced using red-green image enhancement software. Using the length of the horizontal disc diameter (DD) of each eye, the vessel growth was measured from the disc margin up to the vessel tip in fixed quadrants. The rate of vessel growth was the ratio of vessel length to the number of weeks it took to reach this length. The babies were divided into treatment warranting ROP (group 1), low-risk pre-threshold (type II) ROP (group 2,), and no-ROP (group 3) for analysis. The "no-ROP" group acted as normal control. Group 1 was further subdivided into 1A (threshold ROP), IB (aggressive posterior ROP), 1C (hybrid ROP), and ID (high-risk pre-threshold ROP).

Results: Out of 436 eyes, groups 1, 2, and 3 had 238, 108, and 90 eyes, respectively. The mean rate of vascular outgrowth along with 95% confidence interval (CI) was 0.490 [0.487,0.520], 0.612 [0.599, 0.638], and 0.719 [0.703, 0.740] DD/week, respectively, for babies with "treatment warranting," "low risk pre-threshold" and "no ROP" groups, respectively. In our estimate, more than 80% of eyes with a vessel growth rate of 0.54 DD/week or less required treatment.

Conclusion: A rate of retinal vascular growth less than 0.54 DD/week can be used to determine treatment requirements in babies with ROP.
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http://dx.doi.org/10.4103/ijo.IJO_1484_21DOI Listing
April 2022

Parental COVID-19 vaccine hesitancy for children with neurodevelopmental disorders: a cross-sectional survey.

Trop Med Health 2022 Mar 21;50(1):24. Epub 2022 Mar 21.

University of Turku, 20014, Turun yliopisto, Finland.

Background: Little is known about parental coronavirus disease 2019 (COVID-19) vaccine hesitancy in children with neurodevelopmental disorders (NDD). This survey estimated the prevalence and predictive factors of vaccine hesitancy among parents of children with NDD.

Methods: A nationally representative cross-sectional survey was conducted from October 10 to 31, 2021. A structured vaccine hesitancy questionnaire was used to collect data from parents aged ≥ 18 years with children with NDD. In addition, individual face-to-face interviews were conducted at randomly selected places throughout Bangladesh. Multiple logistic regression analysis was conducted to identify the predictors of vaccine hesitancy.

Results: A total of 396 parents participated in the study. Of these, 169 (42.7%) parents were hesitant to vaccinate their children. Higher odds of vaccine hesitancy were found among parents who lived in the northern zone (AOR = 17.15, 95% CI = 5.86-50.09; p < 0.001), those who thought vaccines would not be safe and effective for Bangladeshi children (AOR = 3.22, 95% CI = 1.68-15.19; p < 0.001), those who were either not vaccinated or did not receive the COVID-19 vaccine themselves (AOR = 12.14, 95% CI = 8.48-17.36; p < 0.001), those who said that they or their family members had not tested positive for COVID-19 (AOR = 2.13, 95% CI = 1.07-4.25), and those who did not lose a family member to COVID-19 (AOR = 2.12, 95% CI = 1.03-4.61; p = 0.040). Furthermore, parents who were not likely to believe that their children or a family member could be infected with COVID-19 the following year (AOR = 4.99, 95% CI = 1.81-13.77; p < 0.001) and who were not concerned at all about their children or a family member being infected the following year (AOR = 2.34, 95% CI = 1.65-8.37; p = 0.043) had significantly higher odds of COVID-19 vaccine hesitancy.

Conclusions: Given the high prevalence of vaccine hesitancy, policymakers, public health practitioners, and pediatricians can implement and support strategies to ensure that children with NDD and their caregivers and family members receive the COVID-19 vaccine to fight pandemic induced hazards.
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http://dx.doi.org/10.1186/s41182-022-00415-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8935251PMC
March 2022

Oncogenic human papillomavirus DNA in female sex workers of Bihar, India.

Int J Health Sci (Qassim) 2022 Mar-Apr;16(2):17-26

Mahavir Cancer Sansthan and Research Center Patna, Bihar, India.

Objective: Human papillomavirus (HPV) is a sexually transmitted virus which play a vital role to developing Cervical cancer. It is presumed and accepted that sexually exposed women will have occurred HPV infection at least once in their whole life. Hence, determined the actual cause of transmission of HPV infection in sexually active women with single sex partner that is married women and sexually active women with multiple sex partners that is female sex works (FSWs).

Methods: A total 197 urine sample of FSWs and MW has been utilized through RT-PCR technique for HR-HPV detection. DNA extracted from urine samples and estimation and purification of DNA, used RT-PCR technique for HPV-16 and HPV-18 detection.

Results: The overall prevalence of HR-HPV was detected to be 16.75% (33/197) and 83.25% (164 out 197) negative results reported in both studied subjects. The results analysis of HPV-16 and HPV-18 reveals in which prevalence of HPV-16 was 14.21% (28/197) and HPV-18 was detected 2.53% (5/197) in studied subjects in both study groups. The prevalence of HPV-16 and HPV-18 among MW was high (18.75%) and HPV-16 and HPV-18 was low (14.85%) in FSWs. It did not differ significantly of HR-HPV prevalence in MW who has single sex partner and FSWs who has multiple sex partners.

Conclusion: The study indicates that oncogenic HPV prevalence did not different significantly in multiple sex partners, that is, FSWs and single sex partner, that is, MW. The probability of genital hygiene rather than multiple sexual partners stands more apt as a cause of HPV infection. This study advises to develop more awareness program about genital hygiene in women to reduce the HPV infection and can be prevented from cervical cancer. Hence, genital hygiene may be reducing the burden of HPV infection in women.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905039PMC
March 2022

Oncogenic human papillomavirus DNA in female sex workers of Bihar, India.

Int J Health Sci (Qassim) 2022 Mar-Apr;16(2):17-26

Mahavir Cancer Sansthan and Research Center Patna, Bihar, India.

Objective: Human papillomavirus (HPV) is a sexually transmitted virus which play a vital role to developing Cervical cancer. It is presumed and accepted that sexually exposed women will have occurred HPV infection at least once in their whole life. Hence, determined the actual cause of transmission of HPV infection in sexually active women with single sex partner that is married women and sexually active women with multiple sex partners that is female sex works (FSWs).

Methods: A total 197 urine sample of FSWs and MW has been utilized through RT-PCR technique for HR-HPV detection. DNA extracted from urine samples and estimation and purification of DNA, used RT-PCR technique for HPV-16 and HPV-18 detection.

Results: The overall prevalence of HR-HPV was detected to be 16.75% (33/197) and 83.25% (164 out 197) negative results reported in both studied subjects. The results analysis of HPV-16 and HPV-18 reveals in which prevalence of HPV-16 was 14.21% (28/197) and HPV-18 was detected 2.53% (5/197) in studied subjects in both study groups. The prevalence of HPV-16 and HPV-18 among MW was high (18.75%) and HPV-16 and HPV-18 was low (14.85%) in FSWs. It did not differ significantly of HR-HPV prevalence in MW who has single sex partner and FSWs who has multiple sex partners.

Conclusion: The study indicates that oncogenic HPV prevalence did not different significantly in multiple sex partners, that is, FSWs and single sex partner, that is, MW. The probability of genital hygiene rather than multiple sexual partners stands more apt as a cause of HPV infection. This study advises to develop more awareness program about genital hygiene in women to reduce the HPV infection and can be prevented from cervical cancer. Hence, genital hygiene may be reducing the burden of HPV infection in women.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8905039PMC
March 2022

Comparative plastome data analysis of Dendrosicyos socotranus and Corallocarpus boehmii.

Bioinformation 2021 31;17(7):662-666. Epub 2021 Jul 31.

Department of Environment and Forest Resources, Chungnam National University, Daejeon 34134, Republic of Korea.

Comparison of the Dendrosicyos socotranus and Corallocarpus boehmii (tribe Coniandreae, family Cucurbitaceae) plastome data was of interest. Data on RNA, tRNA, GC%, plastome size, CDS and pseudogene were tabulated for the two species. The total length of 1,57,380 bp and 1,58,744 bp which includes LSC, SSC, IRa, and IRb, while their GC content was 37.1% and 37% respectively. The variation in the length of genes e.g. ndhD, ndhI, rpl22, rpoC2, rps16, rps19, rps8, ycf1and ycf2 noted. Data help to document the genetic differences between usual (climber) with those of tree cucurbits.
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http://dx.doi.org/10.6026/97320630017662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882076PMC
July 2021

Transcriptome characterization of Larrea tridentata and identification of genes associated with phenylpropanoid metabolic pathways.

PLoS One 2022 11;17(3):e0265231. Epub 2022 Mar 11.

Department of Environment and Forest Resources, Chungnam National University, Daejeon, Republic of Korea.

Larrea tridentata (Sesse and Moc. ex DC.) Coville (family: Zygophyllaceae) is an aromatic evergreen shrub with resin-covered leaves, known to use in traditional medicine for diverse ailments. It also has immense pharmacological significance due to presence of powerful phenylpropanoids antioxidant, nordihydroguaiaretic acid (NDGA). The RNA sequence/transcriptome analyses connect the genomic information into the discovery of gene function. Hence, the acquaint analysis of L. tridentata is in lieu to characterize the transcriptome, and to identify the candidate genes involved in the phenylpropanoid biosynthetic pathway. To gain molecular insight, the bioinformatics analysis of transcriptome was performed. The total bases covered 48,630 contigs of length greater than 200 bp and above came out to 21,590,549 with an average GC content of 45% and an abundance of mononucleotide, SSR, including C3H, FAR1, and MADS transcription gene families. The best enzyme commission (EC) classification obtained from the assembled sequences represented major abundant enzyme classes e.g., RING-type E3 ubiquitin transferase and non-specific serine/threonine protein kinase. The KEGG pathway analysis mapped into 377 KEGG different metabolic pathways. The enrichment of phenylpropanoid biosynthesis pathways (22 genes i.e., phenylalanine ammonia-lyase, trans-cinnamate 4-monooxygenase, 4-coumarate-CoA ligase, cinnamoyl-CoA reductase, beta-glucosidase, shikimate O-hydroxycinnamoyl transferase, 5-O-(4-coumaroyl)-D-quinate 3'-monooxygenase, cinnamyl-alcohol dehydrogenase, peroxidase, coniferyl-alcohol glucosyltransferase, caffeoyl shikimate esterase, caffeoyl-CoA O-methyltransferase, caffeate O-methyltransferase, coniferyl-aldehyde dehydrogenase, feruloyl-CoA 6-hydroxylase, and ferulate-5-hydroxylase), and expression profile indicated antioxidant, anti-arthritic, and anticancer properties of L. tridentata. The present results could provide an important resource for squeezing biotechnological applications of L. tridentata.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0265231PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916640PMC
May 2022

Divergent trajectories of antiviral memory after SARS-CoV-2 infection.

Nat Commun 2022 03 10;13(1):1251. Epub 2022 Mar 10.

Peter Medawar Building for Pathogen Research, Nuffield Dept. of Clinical Medicine, University of Oxford, Oxford, UK.

The trajectories of acquired immunity to severe acute respiratory syndrome coronavirus 2 infection are not fully understood. We present a detailed longitudinal cohort study of UK healthcare workers prior to vaccination, presenting April-June 2020 with asymptomatic or symptomatic infection. Here we show a highly variable range of responses, some of which (T cell interferon-gamma ELISpot, N-specific antibody) wane over time, while others (spike-specific antibody, B cell memory ELISpot) are stable. We use integrative analysis and a machine-learning approach (SIMON - Sequential Iterative Modeling OverNight) to explore this heterogeneity. We identify a subgroup of participants with higher antibody responses and interferon-gamma ELISpot T cell responses, and a robust trajectory for longer term immunity associates with higher levels of neutralising antibodies against the infecting (Victoria) strain and also against variants B.1.1.7 (alpha) and B.1.351 (beta). These variable trajectories following early priming may define subsequent protection from severe disease from novel variants.
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http://dx.doi.org/10.1038/s41467-022-28898-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913789PMC
March 2022

Parental coronavirus disease vaccine hesitancy for children in Bangladesh: a cross-sectional study.

F1000Res 2022;11:90. Epub 2022 Jan 25.

Advanced Physiotherapy & Rehab Solution, Women's Children's & General Hospital, Dhaka, 1209, Bangladesh.

Coronavirus disease 2019 (COVID-19) requires mass immunization to control the severity of symptoms and global spread. Data from developed countries have shown a high prevalence of parental COVID-19 vaccine hesitancy. However, parental vaccine hesitancy data in low- and middle-income countries are scarce. This study aimed to assess the prevalence of parental vaccine hesitancy and identify subgroups with higher odds of vaccine hesitancy in parents in Bangladesh. A cross-sectional study was conducted on the parents of children aged <18 years from October 10, 2021 to October 31, 2021. Parents participated in face-to-face interviews in randomly selected locations in Bangladesh using a vaccine hesitancy questionnaire. Factors associated with COVID-19 vaccine hesitancy were identified using binary logistic regression analysis. Data from 2,633 eligible parents were analyzed. Overall, 42.8% reported COVID-19 vaccine hesitancy for their youngest child. The final model suggested the following factors were associated with hesitancy: children's age; parent's age, religion, occupation, monthly household income, permanent address, living location, status of tobacco use, adherence with regular government vaccination programs (other than COVID-19), perceptions of COVID-19 vaccine efficacy among Bangladeshi children, self-vaccination intentions, reported family members' illness or death from COVID-19, and perceived threat of COVID-19 were the independent predictors of parental COVID-19 vaccine hesitancy. Conversely, participants who were not tobacco users, parents who were very likely to believe that their children or family members could be infected with COVID-19 in the following year and who were very concerned about their children or a family member contracting COVID-19 in the next year had significantly lower odds of COVID-19 vaccine hesitancy. Our study suggested that vaccine hesitation varied based on sociodemographic characteristics, religion, behavior, and perceived COVID-19 threat. Therefore, interventions focused on addressing vaccine hesitancy among specific subgroups are warranted.
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http://dx.doi.org/10.12688/f1000research.76181.2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837808.2PMC
March 2022

Proton pump inhibitors' use and risk of iron deficiency anaemia: a systematic review and meta-analysis.

Curr Rev Clin Exp Pharmacol 2022 Mar 7. Epub 2022 Mar 7.

Department of Pharmacy, Mohammed Al-Mana College for Medical Sciences, Abdulrazaq Bin Hammam Street, Al Safa, Dammam 34222 , Saudi Arabia.

Aim: Various research were conducted during last decade, with inconsistent findings regarding iron dearth anaemia (IDA) perils vis-à-vis utilization of proton-pump inhibitors (PPIs). Consequently, recent systematic review and meta-analysis were implemented to evaluate IDA-related perils as it concern the utilization of proton-pump inhibitors.

Methods: The databases of EBSCOhost, PubMed® and Cochrane Central were searched from the research outset until February 28, 2021 purposely to identify all research with objectives that align with the present research investigation. The Newcastle-Ottawa Scale (NOS) was utilized for evaluation of the research investigation standard. The prime (1º) goal of the research was to gauge IDA peril among users of proton-pump inhibitors (PPI). For data processing, RevMan (Review Manager) version 5.4 was employed. Results In total, fourteen investigationa research were employed in this systematic review and meta-analysis. The combined relative risk of nine research exhibited a numerically consequential interrelation betwixt the utilization of proton-pump inhibitors and IDA peril (RR 2.56 [95% CI 1.43-4.61], p < 0.00001). Contemporary systematic review and meta-analysis examination posits that proton-pump inhibitor consumers are prone to greater peril of coming down with IDA in comparison to non-PPI users. Conclusion In keeping with the findings of my research, prescriber physicians should exercise caution when prescribing PPIs to individuals taking it for a long time to avoid the peril of IDA. Additionally, their serum iron level should be checked to ensure that proton-pump inhibitors are safe.
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http://dx.doi.org/10.2174/2772432817666220307121220DOI Listing
March 2022

Oculoplastic Abstracts.

Ophthalmic Plast Reconstr Surg 2022 Mar-Apr 01;38(2):217-218

Ain Shams University, Cairo, Egypt.

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http://dx.doi.org/10.1097/IOP.0000000000002151DOI Listing
March 2022

Effect of assimilate competition during early seed development on the pod and seed growth traits in soybean.

Plant Reprod 2022 Mar 2. Epub 2022 Mar 2.

Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, 40546, USA.

Although the seed remains small in size during the initial stage of seed development (the lag phase), several studies indicate that environment and assimilate supply level manipulations during the lag phase affect the final seed size. However, the manipulations were not only at the lag phase, making it difficult to understand the specific role of the lag phase in final seed size determination. It also remained unclear whether environmental cues are sensed by plants and regulate seed development or if it is simply the assimilate supply level, changed by the environment, that affects the subsequent seed development. We investigated soybean (Glycine max L. Merr.) seed phenotypes grown in a greenhouse using different source-sink manipulations (shading and removal of flowers and pods) during the lag phase. We show that assimilate supply is the key factor controlling flower and pod abortion and that the assimilate supply during the lag phase affects the subsequent potential seed growth rate during the seed filling phase. In response to low assimilate supply, plants adjust flower/pod abortion and lag phase duration to supply the minimum assimilate per pod/seed. Our results provide insight into the mechanisms whereby the lag phase is crucial for seed development and final seed size potential, essential parameters that determine yield.
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http://dx.doi.org/10.1007/s00497-022-00439-2DOI Listing
March 2022

Evidence of dengue virus in eviscerated specimens of panophthalmitis secondary to dengue fever: A possible cause-effect phenomenon.

Indian J Ophthalmol 2022 Mar;70(3):965-969

Smt. Kanuri Santhamma Center for Vitreo-Retinal Diseases, Kallam Anji Reddy Campus, L. V. Prasad Eye Institute, Hyderabad, Telangana, India.

Purpose: To report a retrospective series of three cases of infectious panophthalmitis post-dengue fever with ex vivo confirmation of dengue virusribonucleic acid (RNA) in the tissues of the eye.

Methods: Four eyes of three patients, who were diagnosed with panophthalmitis following dengue fever and who underwent evisceration, were included. All demographic and clinical data were recorded. The eviscerated samples were subjected to direct microscopy, culture for bacteria, fungi, and parasites, and molecular virology (dengue virus [DENV] NS1-specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay).

Results: The time from the development of dengue fever to the occurrence of ocular symptoms was 4.33 ± 1.15 (median 5) days. DENV NS1 RNA, suggestive of the presence of the dengue virus, was confirmed in all evisceration specimens (uveal tissue, cornea). All the patients recovered completely from dengue fever and on follow-up had healthy eviscerated sockets.

Conclusion: Demonstration of the DENV RNA in the eviscerated specimens of panophthalmitis following dengue fever implicates the DENV in the pathophysiology of the ocular infection.
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http://dx.doi.org/10.4103/ijo.IJO_1732_21DOI Listing
March 2022

Correlation of vitreous chamber depth with ocular biometry in high axial myopia.

Indian J Ophthalmol 2022 Mar;70(3):914-920

The Cornea Institute, Kallam Anji Reddy Campus, LV Prasad Eye Institute, Banjara Hills, Hyderabad, Telangana, India.

Purpose: The proportion of axial length (AL) occupied by vitreous chamber depth (VCD), or VCD:AL, consistently correlates to ocular biometry in the general population. Relation of VCD:AL to ocular biometry in high myopia is not known. The purpose of this study is to evaluate the relation of VCD and VCD:AL to ocular biometry of highly myopic eyes.

Methods: This was a cross-sectional retrospective study of records of 214 myopic eyes (<-1 D SE, aged 20-40 years) attending the refractive surgery services. High axial myopia was defined as AL >26.5 mm. Eyes with posterior staphyloma and myopic maculopathy were excluded. Records were assessed for measurements of AL, central corneal thickness (CCT), anterior chamber depth (ACD), lens thickness (LT), white to white diameter (WTW), and vitreous chamber depth (VCD). Groups were formed based on increasing AL, while the sum of CCT, ACD, and LT was recorded as anterior segment depth (AS). The main outcome measure was the correlation of VCD and VCD:AL to ocular biometry. A comparison was also performed based on of degree of axial myopia.

Results: Mean age of the patients was 27.0 ± 5.2 years. VCD showed a very strong correlation with AL (R = 0.98, P < 0.001) but did not correlate to any anterior parameter. VCD:AL showed moderate negative relation with AS (R = -0.43, P < 0.001) and ACD (R = -0.3, P < 0.001), while it had a weakly negative relation with LT (R = -0.18, P = 0.006). VCD:AL showed strong negative relation (R > ~0.7) with AS in all individual groups of AL. Among anterior parameters, WTW showed the most consistent relation with ocular biometry.

Conclusion: VCD:AL is a better correlate of ocular biometry in high myopia as compared to VCD. However, the correlation is weaker than that noted by previous studies done on the general population. Longitudinal studies of VCD:AL in the younger age group is recommended.
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http://dx.doi.org/10.4103/ijo.IJO_1201_21DOI Listing
March 2022
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