Publications by authors named "Mohamed T Shaaban"

4 Publications

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Antibacterial activities of hexadecanoic acid methyl ester and green-synthesized silver nanoparticles against multidrug-resistant bacteria.

J Basic Microbiol 2021 Apr 19. Epub 2021 Apr 19.

Department of Botany and Microbiology, Faculty of Science, Menoufia University, Menoufia, Egypt.

Antibacterial drug resistance is considered one of the biggest threats to human health worldwide, and the overuse of antibiotics accelerates this problem. Multidrug-resistant (MDR) bacteria are becoming harder to treat as the antibiotics used to treat them become less effective. Therefore, it is necessary to evaluate novel methods to control MDR bacteria. In this study, 40 bacterial isolates were collected from diabetic patients. The sensitivity of 40 bacterial isolates to seven antibiotics was evaluated. Four bacterial isolates were resistant to all antibiotic groups. The MDR pathogenic bacteria were selected and identified morphologically and biochemically and confirmed by VITEK® 2 system as follows: Staphylococcus aureus W35, Pseudomonas aeruginosa D31, Klebsiella pneumoniae DF30, and K. pneumoniae B40. Identification of the most resistant P. aeruginosa D31 was confirmed by the sequencing of a 16S ribosomal RNA gene with an accession number (MW241596). The inhibitory activity of eight types of native grown plant extracts against MDR bacteria was studied. Clove alcoholic extract (CAE) showed the highest inhibitory activity against MDR bacteria. Gas chromatography-mass spectrometry analysis of partially purified CAE at 0.9 Rf detected by thin-layer chromatography showed an active compound named hexadecenoic acid methyl ester with the highest antimicrobial effect against clinical pathogenic bacteria. The formation of silver nanoparticles (AgNPs) by CAE was studied. Evaluation of AgNPs was investigated by X-ray diffraction, UV-Vis, and transmission electron microscopy. The antibacterial effect of AgNPs after 2, 4, and 6 days in light and dark conditions was evaluated. Finally, the AgNPs synthesized using CAE possess good inhibition activity against the tested pathogenic bacteria. As a result, the bactericidal components listed above were promising in reducing MDR bacteria and can be used for treatments of bacterial infection and in the development of safe products with a natural base.
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http://dx.doi.org/10.1002/jobm.202100061DOI Listing
April 2021

Bifidobacterium longum Suppresses Murine Colorectal Cancer through the Modulation of oncomiRs and Tumor Suppressor miRNAs.

Nutr Cancer 2019 12;71(4):688-700. Epub 2019 Mar 12.

h h Botany Department, Faculty of Science , Menofiya University , Egypt.

The modulatory role of the Bifidobacterium longum (BL), isolated from women breast milk, on some oncogenic and tumor suppressor miRNAs as well as IL-1β and IL6 targeted-miRNAs was investigated using murine colorectal cancer (CRC) induced on the top of inflammatory ulcerative colitis model. The investigation of the oncomiRs miR-21a and miR-155, which regulate IL-6 and IL-1β expression, indicated that both was depressed by BL-administration in healthy and in CRC-mice. BL-administration induced the tumor suppressor miRNAs (miR-145 and miR-15a) expression in both of the healthy and in CRC-mice. The miR-146a expression, which regulates both of IL-1β and IL-6 expression, was decreased after the BL-administration in both of the healthy and in CRC-mice. In CRC-mice, NF-Kb concentration was elevated, however this NF-Kb induction was diminished after the treatment with BL. BL highly enhanced the IL-1β and IL-6 mRNA and protein concentrations in healthy mice. The administration of BL to CRC-mice resulted in a dramatic increase in IL-1β mRNA and IL-1β concentration, which in contrast was accompanied with a decrease in the IL-6 mRNA and IL-6 concentration. BL-administration resulted in a drop in the aberrant crypt foci number in CRC-mice and increased necrosis and fibrosis of the colon cells. The modulatory influence of B. longum on microRNAs may provide an important therapeutic impact in CRC through inhibition of the proliferation, invasion, apoptosis, and cell cycle of tumor cells.
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http://dx.doi.org/10.1080/01635581.2019.1577984DOI Listing
May 2020

Macroalgal activity against multiple drug resistant Aeromonas hydrophila: A novel treatment study towards enhancement of fish growth performance.

Microb Pathog 2016 Dec 11;101:89-95. Epub 2016 Nov 11.

Botany Department, Faculty of Science, Menoufia University, 32511 Sheben El-Kom, Egypt.

Objective: The aim of this study was to evaluate the efficiency of macroalgal extracts as antibacterial agent against multidrug-resistant (MDR) bacteria isolated from Nile tilapia (Oreochromis niloticus) as well as to enhance the fish growth performance by macroalgae diet application.

Methods: A total of 50 swabs were collected from the diseased organs of tilapia fish including gills, skin, spleen, intestine, liver, kidney and muscle. The isolated bacteria were identified and then confirmed by using VITEK 2. Eight macroalgal species were collected from Abu-Qir, Alexandria coast, Egypt. After determination of their biomass, three solvents were used to prepare algal extracts. The antibacterial activities of different macroalgal extracts were measured against MDR Aeromonas hydrophila 6 (MDRAH6) using well-diffusion method. The mechanism by which macroalgal extract affects MDR bacteria was conducted by using transmission electron microscope (TEM). To evaluate the safety of the promising algal extract, GC-MS was performed to detect the composition of S. vulgare extract. In addition, growth performance was measured as an application of algal extracts into fish feed.

Results: Between eight collected macroalgal species, Sargassum vulgare showed the highest biomass production (53.4 g m). In addition, its ethanolic extract showed the highest significant antibacterial activity with MIC value of 250 μg ml. TEM examination showed distinctive changes in the treated MDRAH6 cells including rupture of the cell wall, leakage of cytoplasmic contents, alterations in the cytoplasm density in addition to totally cell deformation. In addition, GC-MS analysis revealed eleven identified components in S. vulgare ethanolic extract, in which 9,12-octadecadienoyl chloride and hexadecanoic acid methyl ester were dominant (46.6 and 19.7 %, respectively). Furthermore, dietary replacement of fish meal with S. vulgare ethanolic extract significantly enhanced the growth performance and survival of Nile tilapia with a significant reduction in the total bacterial count.

Conclusion: Ethanol extract of the brown macroalga S. vulgare could be a promising antibacterial and a new active agent against MDR A. hydrophila, which could be a major causative agent of Nile tilapia fish diseases. In addition, this study recommended S. vulgare as a natural and effective source to enhance the growth performance of Nile tilapia. In fact, isolation and examination of the individual antibacterial active compounds of the S. vulgar ethanolic extract are under investigation.
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http://dx.doi.org/10.1016/j.micpath.2016.10.026DOI Listing
December 2016

Antimicrobial activity of new 2,4-disubstituted thiazolidinone derivatives.

Z Naturforsch C J Biosci 2009 Nov-Dec;64(11-12):785-9

Photochemistry Department, National Research Center, El Dokki, Cairo, Egypt.

A number of new disubstituted 2,5-thiazolidinone derivatives were synthesized and tested for their antimicrobial activity against Bacillus subtilis (Gram-positive), Pseudomonas aeruginosa (Gram-negative), and Streptomyces species (Actinomycetes). They displayed different degrees of antimicrobial activities or inhibitory actions.
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http://dx.doi.org/10.1515/znc-2009-11-1205DOI Listing
April 2010