Dr. Mohamed Abdo Rizk, PhD - Mansoura University - Assistant Professor

Dr. Mohamed Abdo Rizk

PhD

Mansoura University

Assistant Professor

Obihiro-Japan, Hokkaido | Japan

Main Specialties: Infectious Disease, Internal Medicine

Additional Specialties: Internal medicine and Infectious diseases

ORCID logohttps://orcid.org/0000-0002-5250-6172


Top Author

Dr. Mohamed Abdo Rizk, PhD - Mansoura University - Assistant Professor

Dr. Mohamed Abdo Rizk

PhD

Introduction

Dr. Mohamed Abdo Rizk holds a DVM degree from Faculty of Veterinary Medicine, Mansoura University. He obtained his Master degree in 2011 in veterinary internal medicine from School of Veterinary Medicine, Mansoura University and obtained his joint PhD (Egypt/Japan) at 2016 in internal medicine from Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. Dr. Mohamed Abdo Rizk has more than 8 years of experience in veterinary internal medicine and infectious diseases. Beside his research experience in that area, he has 9 years of teaching experience primarily related to teaching veterinary internal medicine and infectious diseases to DVM students. He has many publications in national and international scientific journals. He received the prize of the best PhD thesis in Mansoura University at 2016. He trained in horse clinic and small clinic in University of Veterinary Medicine Hannover, Hannover, Germany. He worked as foreign visiting researcher at National Research Center of Protozoan Diseases (NRCPD), Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. Currently he is assistant professor of internal medicine and infectious diseases at Faculty of Veterinary Medicine, Mansoura University.

Primary Affiliation: Mansoura University - Obihiro-Japan, Hokkaido , Japan

Specialties:

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View Dr. Mohamed Abdo Rizk’s Resume / CV

Education

Jun 2016 - Dec 2016
Obihiro Chikusan Daigaku
Dr./Foreign Visiting Researcher
National Research Center for Protozoan Diseases
Mar 2016
Mansoura University Faculty of Veterinary Medicine
Dr./Assistant Professor
Internal Medicine and Infectious Diseases

Experience

Nov 2016
Best PhD thesis in Mansoura University, Egypt

Publications

23Publications

200Reads

1424Profile Views

7PubMed Central Citations

Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on piroplasm parasites.

Parasit Vectors 2019 Jan 16;12(1):37. Epub 2019 Jan 16.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan.

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http://dx.doi.org/10.1186/s13071-019-3296-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335684PMC
January 2019
3 Reads
3.430 Impact Factor

The first molecular detection of Clostridium perfringens from pneumonic cases associated with foot and mouth disease in cattle and buffalo in Egypt.

Trop Anim Health Prod 2018 Nov 28. Epub 2018 Nov 28.

Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.

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http://link.springer.com/10.1007/s11250-018-1763-8
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http://dx.doi.org/10.1007/s11250-018-1763-8DOI Listing
November 2018
13 Reads
0.820 Impact Factor

Dietary flaxseed oil inhibits kidney NF-kappa B activation and pro-inflammatory cytokine expression in cisplatin-treated rats

Kheira, H.S., El-Sayed, S.A.ES., Elsayed, G.R. et al. Comp Clin Pathol (2018). https://doi.org/10.1007/s00580-018-2871-6

Comparative Clinical Pathology

The current study explores the anti-inflammatory effect of flaxseed oil (FXO) on cisplatin (CP)-induced renal damage. Rats prefed with normal experimental diets (normal/FXO diet) for 10 days were administered 30 mg kg−1 and 50 mg kg−1 i.p. CP in a single dose, while still on the experimental diets. Significant improvement in kidney function test and antioxidant enzyme activities in rats treated with combination therapy (FXO + CP) was detected. Interestingly, treatment by FXO resulted in significant downregulation (P < 0.05) in interleukin (IL)-6 and IL-1β expression in the renal tissue in comparison with CP- treated rats. Moreover, administration of FXO yielding negative and mild positive brown immunostain for tumor necrosis factor- alpha (TNF-α) or mild and moderate positive brown immunostain for nuclear factor Kappa B (NF-kB) in the renal tissue of rats treated with 3 mg kg−1 CP + FXO and 5 mg kg−1 CP + FXO, respectively. The obtained results highlight the ameliorative anti- inflammatory effect of FXO in the renal damage associated with CP administration and confirm the necessity and usefulness of FXO dietary supplement when using CP in the treatment of cancer patients.

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November 2018
2 Reads

The first molecular detection of Clostridium perfringens from pneumonic cases associated with foot and mouth disease in cattle and buffalo in Egypt

Elgioushy, M., Rizk, M.A., El-Adl, M. et al. Trop Anim Health Prod (2018). https://doi.org/10.1007/s11250-018-1763-8

Tropical Animal Health and Production

Panting syndrome and respiratory infection have been recorded in complicated cases of foot and mouth disease (FMD) in cattle. However, investigations on the causative agents of respiratory disease in such cases are scarce. In this study, 30 animals (13 buffalo and 17 cattle) suffering from respiratory distress associated with signs of FMD were examined. Serum samples were collected and FMD infection was confirmed. Bacteriological examination of lungs from eight necropitized cases revealed the presence of C. perfringens. Multiplex polymerase chain reaction (mPCR) was performed on the positive samples followed by sequencing analysis. The alpha toxin gene (plc) of C. perfringens was identified in six cases. The present investigation highlights the role of clostridial infection as a complication of FMD in cattle and buffalo. This is the first report identifying the C. perfringens toxins from lung of animals with respiratory distress associated with FMD infection.

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November 2018
2 Reads

An epidemiological survey of bovine viral diarrhea infection in calves in Egypt with identification of high prevalence of persistent infected animals

Atwa, S.M., Emad, Y.E., Rizk, M.A. et al. Comp Clin Pathol (2018). https://doi.org/10.1007/s00580-018-2867-2

Comparative Clinical Pathology

In the present study, we identified the persistent infection by bovine viral diarrhea virus (BVDV) in 45 clinically suspected persistent infected (PI) calves and 260 apparently healthy calves from Damietta governorate (North) in Egypt during 2016 using enzyme- linked immunosorbent assay (ELISA), serum neutralization test (SNT), immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR). The identification of PI calves was performed after vaccination by CattleMaster® 4. The age of PI calves in the current study was ranged from 2 to 4 months (n = 7) and from 5 to 8 months (n = 38). ELISA revealed the infection in 4 clinically suspected PI calves (57.14%) aged 2 to 4 months. While the infection did not approve in any of the calves within this age using RT-PCR, for calves aged from 5 to 8 months old, ELISA showed the infection in 30 calves (78.94%), and from these calves, the infection was confirmed in 6 calves (15.79%) only using RT-PCR. For apparently clinically healthy animals, 11 transiently infected calves were identified by IHC (positive), SNT (positive), and RT-PCR (negative) at 2 months’ post-vaccination. Collectively, the persistent infection was confirmed in 9 calves (2.95%) out of 305 calves. In summary, our study detected a high prevalence of PI calves with BVDV in Damietta province in Egypt, which highlights the importance of introducing effective prevention and control strategies throughout Egypt to minimize the prevalence of BVDV.

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November 2018
4 Reads

Inhibitory effects of fluoroquinolone antibiotics on and , blood parasites of veterinary and zoonotic importance.

Infect Drug Resist 2018 28;11:1605-1615. Epub 2018 Sep 28.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan,

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https://www.dovepress.com/inhibitory-effects-of-fluoroquinol
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http://dx.doi.org/10.2147/IDR.S159519DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6166754PMC
September 2018
5 Reads

Inhibitory effects of fluoroquinolone antibiotics on Babesia divergens and Babesia microti, blood parasites of veterinary and zoonotic importance

Rizk MA, AbouLaila M, El-Sayed SAE, Guswanto A, Yokoyama N, et al. Inhibitory effects of fluoroquinolone antibiotics on Babesia divergens and Babesia microti, blood parasites of veterinary and zoonotic importance. Infect Drug Resist. 2018:11: 1605–1615

Infection and Drug Resistance

Aim: This study aimed to evaluate the inhibitory effects of fluoroquinolone antibiotics, includ- ing enrofloxacin, enoxacin, trovafloxacin, norfloxacin, and ofloxacin, on the in vitro and in vivo growth of Babesia divergens and Babesia microti parasites, respectively. Materials and methods: The in vitro and in vivo inhibitory effects of fluoroquinolone antibiotics against B. divergens and B. microti, respectively were evaluated using fluorescence- based assay. Additionally, combination therapies of highly effective fluoroquinolone antibiotics (enrofloxacin, enoxacin, and trovafloxacin) with diminazene aceturate, luteolin, or pyronaridine tetraphosphate were tested on the in vitro cultures of B. divergens. Results: Enrofloxacin, trovafloxacin, and enoxacin were the most effective fluoroquinolones against the in vitro growth of B. divergens, followed by norfloxacin and ofloxacin. Further- more, a combination of enoxacin or trovafloxacin with either diminazene aceturate, luteolin, or pyronaridine tetraphosphate significantly enhanced the inhibitory effect on the growth of B. divergens in in vitro cultures. In mice infected by B. microti, enoxacin and diminazene aceturate combination therapy exhibited a potential antibabesial effect. Conclusion: These results suggest that safe and cheap fluoroquinolone, such as enoxacin, might be used for the treatment of clinical cases caused by Babesia spp. in animals or humans. Keywords: Babesia, fluoroquinolones, in vitro, in vivo

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September 2018
6 Reads

The effects of nitidine chloride and camptothecin on the growth of Babesia and Theileria parasites.

Ticks Tick Borne Dis 2018 07 27;9(5):1192-1201. Epub 2018 Apr 27.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture Veterinary Medicine, Nishi 2 Sen-11, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address:

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https://www.researchgate.net/publication/262979477_Evaluatio
Web Search
https://linkinghub.elsevier.com/retrieve/pii/S1877959X173058
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http://dx.doi.org/10.1016/j.ttbdis.2018.04.019DOI Listing
July 2018
18 Reads
2.720 Impact Factor

17-DMAG inhibits the multiplication of several Babesia species and Theileria equi on in vitro cultures, and Babesia microti in mice.

Int J Parasitol Drugs Drug Resist 2018 04 1;8(1):104-111. Epub 2018 Mar 1.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13 Inada-cho, Obihiro 080-8555, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.ijpddr.2018.02.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114103PMC
April 2018
10 Reads
2.514 Impact Factor

Molecular identification and antigenic characterization of Babesia divergens Erythrocyte Binding Protein (BdEBP) as a potential vaccine candidate.

Parasitol Int 2017 Dec 22;66(6):721-726. Epub 2017 Jul 22.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.parint.2017.07.004DOI Listing
December 2017
21 Reads
2.111 Impact Factor

Comparative therapeutic effect of steroidal and non-steroidal anti-inflammatory drugs on pro-inflammatory cytokine production in water buffalo calves (Bubalus bubalis) naturally infected with bronchopneumonia: a randomized clinical trial.

Trop Anim Health Prod 2017 Dec 22;49(8):1723-1731. Epub 2017 Aug 22.

Department of Animal Medicine, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt.

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http://link.springer.com/10.1007/s11250-017-1383-8
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http://dx.doi.org/10.1007/s11250-017-1383-8DOI Listing
December 2017
57 Reads
0.820 Impact Factor

Evaluation of Ascorbic Acid in Combination of Ivermectin in Augmentation the Recovery from Juvenile Generalized Demodicosis in Dogs: A Randomized Clinical Trial

Rizk, M.A., Abdalla, A.A., El-Sayed, S.A.E.S., 2017. Evaluation of Ascorbic Acid in Combination of I

PSM Veterinary Research

The clinical form of canine juvenile generalized demodicosis is usually related genetically with immune deficiency. In this regard, in the current study we have evaluated the impact of immunostimulant agent as ascorbic acid (AA) on the rapid recovery of dogs naturally affected by generalized demodicosis. To achieve this purpose, twenty- eight German Shepherd male dogs were used in this study and divided randomly into two groups, then received daily oral dose of ivermectin (IVR) 1% (0.5 mg kg-1) alone and IVR 1% in combination with AA (500 mg per animal, twice daily) for two months, respectively. Total mite numbers, egg counts, eosinophil counts and skin lesion score were used for assessment the treatment efficacy after 30, 60, 90, and 120 days subsequent to the initial treatment. Seventeen dogs (IVR (n = 8), and IVR + AA (n = 9)) completed the full length of experiment (4- months) as 11 dogs were withdrawn due to various causes. Out of 17 dogs completing the 4 months trial, two dogs treated with IVR + AA combination therapy achieved the parasitological cure. The results revealed rapid and marked reduction in the total mite numbers and eosinophil counts treated with combination therapy in comparison with those administrated ivermectin alone. These findings exhibited the potential anti-Demodex effect of ivermectin when administrated in combination with immunostimulant agent and highlight the impact of an inexpensive, available and immunomodulatory agent as ascorbic acid in treatment of canine juvenile generalized demodicosis.

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November 2017
12 Reads

Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice.

Sci Rep 2017 Oct 16;7(1):12774. Epub 2017 Oct 16.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, 080-8555, Japan.

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http://dx.doi.org/10.1038/s41598-017-13052-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5643553PMC
October 2017
18 Reads
1 Citation
5.080 Impact Factor

Evaluation of the inhibitory effect of N-acetyl-L-cysteine on Babesia and Theileria parasites.

Exp Parasitol 2017 Aug 24;179:43-48. Epub 2017 Jun 24.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.exppara.2017.06.003DOI Listing
August 2017
16 Reads
1 Citation
1.860 Impact Factor

A comparative study on selected APP, alkaline phosphatase and lactate dehydrogenase activities in buffalo and cow with subclinical mastitis

Comp Clin Pathol

The aim of this study was to evaluate the comparative changes of selected acute-phase proteins (APPs), alkaline phos- phatase (ALP), and lactate dehydrogenase (LDH) activities in buffalos and cows with subclinical mastitis. For this purpose, a total of 139 buffalos (Bubalus bubalis) and 146 cows (Holstein or Friesian) from Dakahlia governorate in Egypt were studied. Seventy-six cows and 49 buffalos exhibited subclinical mastitis with positive California mastitis test (CMT) and no clinical signs of mastitis. Results revealed that the most dominant isolated bacteria were Staphylococcus spp. in both buffalo and cow fellowed by Streptococcus spp. and E.coli. Statistical significant differences were observed in SCC, HP level, and LDH activity between buffalos with different bacterial isolates. Milk HP level showed significant differences (P < 0.05) between clinically health buffalos and cows and those infected with different isolat- ed pathogen. In conclusion, similar to cows, Hp is a sensitive marker of subclinical mastitis in buffalo, and they may be advan- tageous to clarify the host response to the inflammation of mam- mary gland in clinical research. However, additional studies on awider range of APPs and specific pathogens in buffalos are re- quired to explore the response pattern to mastitis in such species.

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February 2017
14 Reads

Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond

Van Voorhis WC, Adams JH, Adelfio R, Ahyong V, Akabas MH, Alano P, et al. (2016) Open Source Drug Di

PLOS PATHOGENS

A major cause of the paucity of new starting points for drug discovery is the lack of interac- tion between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of medicinal chemistry is still largely within industry. Open source drug discovery, with sharing of information, is clearly a first step towards overcoming this gap. But the interface could especially be bridged through a scale-up of open sharing of physical compounds, which would accelerate the finding of new starting points for drug discovery. The Medicines for Malaria Venture Malaria Box is a collection of over 400 com- pounds representing families of structures identified in phenotypic screens of pharmaceuti- cal and academic libraries against the Plasmodium falciparum malaria parasite. The set has now been distributed to almost 200 research groups globally in the last two years, with the only stipulation that information from the screens is deposited in the public domain. This paper reports for the first time on 236 screens that have been carried out against the Malaria Box and compares these results with 55 assays that were previously published, in a format that allows a meta-analysis of the combined dataset. The combined biochemical and cellu- lar assays presented here suggest mechanisms of action for 135 (34%) of the compounds active in killing multiple life-cycle stages of the malaria parasite, including asexual blood, liver, gametocyte, gametes and insect ookinete stages. In addition, many compounds dem- onstrated activity against other pathogens, showing hits in assays with 16 protozoa, 7 hel- minths, 9 bacterial and mycobacterial species, the dengue fever mosquito vector, and the NCI60 human cancer cell line panel of 60 human tumor cell lines. Toxicological, pharmaco- kinetic and metabolic properties were collected on all the compounds, assisting in the selec- tion of the most promising candidates for murine proof-of-concept experiments and medicinal chemistry programs. The data for all of these assays are presented and analyzed to show how outstanding leads for many indications can be selected. These results reveal the immense potential for translating the dispersed expertise in biological assays involving human pathogens into drug discovery starting points, by providing open access to new fam- ilies of molecules, and emphasize how a small additional investment made to help acquire and distribute compounds, and sharing the data, can catalyze drug discovery for dozens of different indications. Another lesson is that when multiple screens from different groups are run on the same library, results can be integrated quickly to select the most valuable starting points for subsequent medicinal chemistry efforts.

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July 2016
14 Reads

Large-scale drug screening against Babesia divergens parasite using a fluorescence-based high-throughput screening assay

Veterinary Parasitology

The validation of a fluorescence-based high-throughput screening (HTS) assay for determining the effica- cies of large chemical libraries against Babesia divergens (bovine strain) in in vitro cultures was evaluated in this study. Hematocrits (HCTs) of 2.5%, 5%, and 10% were used for the in vitro culture at 1% parasitemia without daily replacement of the medium. Linearity and HTS assay results revealed that the best HCTs were 5% and 10%. The obtained IC50 values of diminazene aceturate, either by fluorescence-based HTS assay with and without daily replacement of medium or by fluorescence- and microscopy-based methods, did not differ significantly at 5% HCT. Actinonin and chloroquine diphosphate were the most effective drugs against the in vitro growth of B. divergens, followed by pyronaridine tetraphosphate- and luteolin- treated cultures. On contrary, tetracycline hydrochloride and (−)-epigallocatechin-3-gallate from green tea exhibited poor activity as compared with diminazene aceturate (positive control drug). The data indi- cated that 5% HCT without daily replacement of the culture medium mixed with bovine serum in vitro using a fluorescence-based HTS assay creates the best conditions for large-scale drug screening against B. divergens that infect cattle.

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July 2016
13 Reads

Evaluation of in vitro inhibitory effect of enoxacin on Babesia and Theileria parasites

Experimental Parasitology

Enoxacin is a broad-spectrum 6-fluoronaphthyridinone antibacterial agent (fluoroquinolones) structur- ally related to nalidixic acid used mainly in the treatment of urinary tract infections and gonorrhea. Also it has been shown recently that it may have cancer inhibiting effect. The primary antibabesial effect of Enoxacin is due to inhibition of DNA gyrase subunit A, and DNA topoisomerase. In the present study, enoxacin was tested as a potent inhibitor against the in vitro growth of bovine and equine Piroplasms. The in vitro growth of five Babesia species that were tested was significantly inhibited (P < 0.05) by micro molar concentrations of enoxacin (IC50 values 1⁄4 33.5, 15.2, 7.5 and 23.2 mM for Babesia bovis, Babesia bigemina, Babesia caballi, and Theileria equi, respectively). Enoxacin IC50 values for Babesia and Theileria parasites were satisfactory as the drug is potent antibacterial drug with minimum side effects. Therefore, enoxacin might be used for treatment of Babesiosis and Theileriosis especially in case of mixed infections with bacterial diseases or incase of animal sensitivity against diminazin toxicity.

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December 2015
14 Reads

Cocktail of Theileria equi antigens for detecting infection in equines

Asian Pac J Trop Biomed 2015; 5(12):930-933

Asian Pacific Journal of Tropical Biomedicine

Objective: To use two diagnostic antigens belonging to the frequently associated in Theileria domain, Theileria equi (T. equi) protein 82 (Te 82) and T. equi 104 kDa microneme-rhoptry antigen precursor (Te 43), to diagnose T. equi infection in horses as compared with equi merozoite antigen-2 (EMA-2). Methods: In the current study, we applied a cocktail ELISA containing two antigens (EMA-2 + Te 82) to diagnose T. equi infection either in experimentally infected horses or in field infection. Results: Our findings have revealed that a cocktail formula of EMA-2 + Te 82 provided a more practical and sensitive diagnostic candidate for diagnosing T. equi infection in horses as compared with Te 82 or Te 43 alone. Conclusions: The ELISA technique using a cocktail formula of EMA-2 + Te 82 offers a practical and sensitive diagnostic tool for diagnosing T. equi infection in horses and using of this promising cocktail formula will be applicable for epidemiological surveys and will help control the infection in horses.

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October 2015
15 Reads

An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt.

Parasitol Int 2015 Feb 14;64(1):79-85. Epub 2014 Oct 14.

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Electronic address:

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http://dx.doi.org/10.1016/j.parint.2014.10.002DOI Listing
February 2015
20 Reads
5 Citations
2.111 Impact Factor

REFERENCES CONTENT ALERTS Evaluation of a Fluorescence-Based Method for Antibabesial Drug Screening

Antimicrobial Agents and Chemotherapy

In vitro evaluation of chemotherapeutic agents against Babesia and Theileria parasites has become routine, and the effectiveness of these chemicals is usually determined by comparing the parasitemia dynamics of untreated and treated parasites. Although microscopy is widely used to calculate parasitemia, several disadvantages are associated with this technique. The present study evaluated a fluorescence-based method using SYBR green I stain (SG I) to screen antibabesial agents in in vitro cultures of Babe- sia bovis. The linearity between relative fluorescence units (RFU) and parasitemia was found to be well correlated with a 0.9944 goodness-of-fit (r2) value. Subsequently, 50% inhibitory concentration (IC50) values were calculated for 3 antiprotozoan agents, diminazene aceturate, nimbolide, and gedunin, by this method. For diminazene aceturate and nimbolide, the IC50s determined by the fluorescence-based method (408 nM and 8.13

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June 2014
16 Reads

A comparative study on selected acute-phase proteins (APPs) and immunoglobulins in buffalo and bovine calves with respiratory disease

DOI 10.1007/s00580-014-1933-7

Comp Clin Pathol

Cytokines play an important role in innate immune response to diseases in bovine calves. The objective of the present study was to assess the comparative changes of se- lected acute-phase proteins (APPs) and immunoglobulins in buffalo and bovine calves with respiratory disease. For this purpose, a total of 65 calves (28 buffalo and 37 bovine) were studied. Of all, 45 calves (21 buffalo and 24 bovine) were diagnosed to have respiratory disease. In addition, 20 clinical- ly healthy calves (7 buffalo and 13 bovine) were randomly selected as a control group. Clinical and hematological exam- inations as well as measurement of haptoglobin (Hp), fibrin- ogen (Fb), IgG, IgA, total protein (TP), albumin, and globulin were carried out. Clinically, in acute respiratory disease, buf- falo calves had lower clinical index scores (p<0.05) than bovine calves. In both buffalo and bovine calves, Fb and Hp levels were increased significantly (p<0.05) in acute respira- tory disease compared with chronic cases. However, IgA, IgG, and albumin levels showed a significant decrease (p < 0.05). Moreover, buffalo calves had a significant increase (p < 0.05) of Hp and IgG compared with bovine calves. Total protein and globulin levels showed a significant decrease (p < 0.05) in calves with chronic respiratory diseases. In con- clusion, Hp and IgG response in buffalo calves with respira- tory disease seems to differ from bovine calves. Further stud- ies need to be done on experimental respiratory infection in buffalo calves to elucidate the specific acute-phase response.

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April 2014
12 Reads

Top co-authors

Naoaki Yokoyama
Naoaki Yokoyama

National Research Center for Protozoan Diseases

9
Ikuo Igarashi
Ikuo Igarashi

National Research Center for Protozoan Diseases

9
Azirwan Guswanto
Azirwan Guswanto

Obihiro University of Agriculture and Veterinary Medicine

4
Mahmoud Aboulaila
Mahmoud Aboulaila

National Research Center for Protozoan Diseases

4
Thillaiampalam Sivakumar
Thillaiampalam Sivakumar

Obihiro University of Agriculture and Veterinary Medicine

4
Bumduuren Tuvshintulga
Bumduuren Tuvshintulga

National Research Center for Protozoan Diseases

3
Dickson Stuart Tayebwa
Dickson Stuart Tayebwa

Central Diagnostic Laboratory

3
Arifin Budiman Nugraha
Arifin Budiman Nugraha

National Research Center for Protozoan Diseases

2

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